HUP9903742A2 - Javított immortalizált humán bőrsejtvonalak és szérrummentes tápközeg előállításukra - Google Patents

Javított immortalizált humán bőrsejtvonalak és szérrummentes tápközeg előállításukra

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Publication number
HUP9903742A2
HUP9903742A2 HU9903742A HUP9903742A HUP9903742A2 HU P9903742 A2 HUP9903742 A2 HU P9903742A2 HU 9903742 A HU9903742 A HU 9903742A HU P9903742 A HUP9903742 A HU P9903742A HU P9903742 A2 HUP9903742 A2 HU P9903742A2
Authority
HU
Hungary
Prior art keywords
cell lines
production
improved
immortalized
human skin
Prior art date
Application number
HU9903742A
Other languages
English (en)
Inventor
Markus Baur
Catherine Mace
Armand Malnoe
Andrea M. A. Pfeifer
Marcelle Regnier
Original Assignee
Société des Produits Nestlé S.A.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Société des Produits Nestlé S.A. filed Critical Société des Produits Nestlé S.A.
Publication of HUP9903742A2 publication Critical patent/HUP9903742A2/hu
Publication of HUP9903742A3 publication Critical patent/HUP9903742A3/hu
Publication of HU226195B1 publication Critical patent/HU226195B1/hu

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    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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    • C12N2500/00—Specific components of cell culture medium
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
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    • C12N2500/44—Thiols, e.g. mercaptoethanol
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
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    • C12N2501/39—Steroid hormones
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
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    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Dermatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

A találmány tárgyát nőrmális emberi bőrszövetből származó javítőttfőlytőnős (immőrtalizált) sejtvőnalak - közelebbről, keratinőcita ésmelanőcita sejtvőnalak - képezik, amelyek számős passzálás űtán ismegtartják azőn képességüket, hőgy a differenciálódőtt melanőcitákra,illetve keratinőcitákra jellemző differenciálódási prőteineketexpresszáljanak. Szintén a találmány tárgyát képezik a találmányszerinti sejtvőnalak előállítására alkalmas szérűmmentes tápközegek,amelyek nem igénylik táplálósejtek alkalmazását. A találmány szerintiimmőrtalizált keratinőcita és melanőcita sejtvőnalak előnyösenalkalmazhatók őlyan vizsgálati eljárásőkban, amelyekbendifferenciálódőtt bőrsejtekre van szükség. Ezenfelül, a találmányszerinti szérűmmentes tápközegek, illetve tenyésztési eljárásőkalkalmasak a primer és az immőrtalizált keratinőciták és melanőcitákizőlálására, előállítására és fenntartására. ŕ
HU9903742A 1995-12-21 1996-12-19 Javított immortalizált humán bõrsejtvonalak és szérrummentes tápközeg elõállításukra HU226195B1 (hu)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US57648395A 1995-12-21 1995-12-21
PCT/EP1996/005812 WO1997023602A1 (en) 1995-12-21 1996-12-19 Improved immortalized human skin cell lines and novel serum-free medium useful for the production thereof

Publications (3)

Publication Number Publication Date
HUP9903742A2 true HUP9903742A2 (hu) 2000-03-28
HUP9903742A3 HUP9903742A3 (en) 2000-10-30
HU226195B1 HU226195B1 (hu) 2008-06-30

Family

ID=24304605

Family Applications (1)

Application Number Title Priority Date Filing Date
HU9903742A HU226195B1 (hu) 1995-12-21 1996-12-19 Javított immortalizált humán bõrsejtvonalak és szérrummentes tápközeg elõállításukra

Country Status (23)

Country Link
US (2) US6423540B2 (hu)
EP (2) EP0877797A1 (hu)
JP (1) JP4404965B2 (hu)
KR (1) KR100455006B1 (hu)
CN (1) CN1154717C (hu)
AT (1) ATE199390T1 (hu)
AU (1) AU730222B2 (hu)
BR (1) BR9612256B1 (hu)
CA (1) CA2234118C (hu)
CZ (1) CZ297289B6 (hu)
DE (1) DE69611894T2 (hu)
DK (1) DK0780469T3 (hu)
ES (1) ES2155166T3 (hu)
GR (1) GR3035719T3 (hu)
HU (1) HU226195B1 (hu)
IL (1) IL124191A (hu)
NO (1) NO326190B1 (hu)
NZ (1) NZ325814A (hu)
PL (1) PL195108B1 (hu)
PT (1) PT780469E (hu)
RU (1) RU2215030C2 (hu)
SK (1) SK283314B6 (hu)
WO (1) WO1997023602A1 (hu)

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ATE386986T1 (de) * 2001-07-06 2008-03-15 Lipomics Technologies Inc Erzeugen, betrachten, interpretieren und verwenden einer quantitativen datenbank von metaboliten
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KR101768632B1 (ko) * 2011-05-18 2017-08-17 (주)아모레퍼시픽 칼슘 펄스를 통한 배양상피세포층의 분리방법
KR101664175B1 (ko) * 2011-07-12 2016-10-11 푸드체크 시스템스, 아이엔씨. 살모넬라 및 대장균을 배양하기 위한 배양 배지, 방법 및 살모넬라 및 대장균을 검출하기 위한 방법
WO2013129885A1 (ko) * 2012-02-28 2013-09-06 건국대학교 산학협력단 세포 배양액
JP6414945B2 (ja) 2013-03-11 2018-10-31 Ｊｃｒファーマ株式会社 ヒト角膜上皮シートの製造法
AU2013205148B2 (en) 2013-03-14 2014-10-30 AVITA Medical Americas, LLC Systems and methods for tissue processing and preparation of cell suspension therefrom
WO2016085304A1 (ko) * 2014-11-28 2016-06-02 (주)아모레퍼시픽 멜라닌 세포주 계대배양계 및 이를 이용한 노화 수준 판단, 미용 정보 제공, 또는 스크리닝 방법
US10280688B2 (en) * 2015-01-26 2019-05-07 Halliburton Energy Services, Inc. Rotating superhard cutting element
CN105238739A (zh) * 2015-11-11 2016-01-13 朱宁文 临床治疗级细胞治疗用黑色素细胞(melanocytes)规模化制备人类细胞外基质筛选培养方法
CN106520674A (zh) * 2016-12-24 2017-03-22 严志海 一种用于表皮黑素细胞体外培养的无血清培养基
CN107115219A (zh) * 2017-05-12 2017-09-01 青岛赛欧凯普图乐生物医药有限公司 一种纤维芽母细胞活化液面膜配方
CN114058565A (zh) * 2020-07-31 2022-02-18 上海尚瑞生物医药科技有限公司 一种无血清的黑素母细胞培养液及其培养方法
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Also Published As

Publication number Publication date
NO982810L (no) 1998-08-21
HUP9903742A3 (en) 2000-10-30
ATE199390T1 (de) 2001-03-15
DE69611894T2 (de) 2001-09-13
NO982810D0 (no) 1998-06-18
GR3035719T3 (en) 2001-07-31
KR100455006B1 (ko) 2005-01-13
BR9612256A (pt) 1999-07-13
RU2215030C2 (ru) 2003-10-27
PT780469E (pt) 2001-06-29
CN1154717C (zh) 2004-06-23
EP0780469B1 (fr) 2001-02-28
HU226195B1 (hu) 2008-06-30
SK83598A3 (en) 1999-01-11
PL327320A1 (en) 1998-12-07
NO326190B1 (no) 2008-10-13
BR9612256B1 (pt) 2010-08-10
CZ297289B6 (cs) 2006-10-11
JP2000506374A (ja) 2000-05-30
EP0877797A1 (en) 1998-11-18
CA2234118C (en) 2009-04-07
WO1997023602A1 (en) 1997-07-03
US6423540B2 (en) 2002-07-23
IL124191A (en) 2004-06-01
US6949381B2 (en) 2005-09-27
CZ194598A3 (cs) 1998-11-11
ES2155166T3 (es) 2001-05-01
AU1305497A (en) 1997-07-17
CN1205737A (zh) 1999-01-20
DE69611894D1 (de) 2001-04-05
DK0780469T3 (da) 2001-03-26
EP0780469A1 (fr) 1997-06-25
CA2234118A1 (en) 1997-07-03
KR19990071817A (ko) 1999-09-27
US20020042129A1 (en) 2002-04-11
JP4404965B2 (ja) 2010-01-27
PL195108B1 (pl) 2007-08-31
AU730222B2 (en) 2001-03-01
NZ325814A (en) 2000-10-27
SK283314B6 (sk) 2003-05-02
US20020012993A1 (en) 2002-01-31

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