DK0780469T3 - Udødeliggjorte cellelinier stammende fra normalt menneskeligt hudvæv og serumfrit vækstmedium til dyrkning af sådanne - Google Patents

Udødeliggjorte cellelinier stammende fra normalt menneskeligt hudvæv og serumfrit vækstmedium til dyrkning af sådanne

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Publication number
DK0780469T3
DK0780469T3 DK96203641T DK96203641T DK0780469T3 DK 0780469 T3 DK0780469 T3 DK 0780469T3 DK 96203641 T DK96203641 T DK 96203641T DK 96203641 T DK96203641 T DK 96203641T DK 0780469 T3 DK0780469 T3 DK 0780469T3
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DK
Denmark
Prior art keywords
cell lines
serum
human skin
normal human
skin tissue
Prior art date
Application number
DK96203641T
Other languages
English (en)
Inventor
Markus Baur
Catherine Mace
Armand Malnoe
Andrea M A Pfeifer
Marcelle Regnier
Original Assignee
Nestle Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nestle Sa filed Critical Nestle Sa
Application granted granted Critical
Publication of DK0780469T3 publication Critical patent/DK0780469T3/da

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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    • C12N2500/00—Specific components of cell culture medium
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    • C12N2500/20—Transition metals
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/38—Vitamins
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    • C12N2500/44—Thiols, e.g. mercaptoethanol
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/46—Amines, e.g. putrescine
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/80—Neurotransmitters; Neurohormones
    • C12N2501/81—Adrenaline
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Dermatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DK96203641T 1995-12-21 1996-12-19 Udødeliggjorte cellelinier stammende fra normalt menneskeligt hudvæv og serumfrit vækstmedium til dyrkning af sådanne DK0780469T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US57648395A 1995-12-21 1995-12-21

Publications (1)

Publication Number Publication Date
DK0780469T3 true DK0780469T3 (da) 2001-03-26

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Application Number Title Priority Date Filing Date
DK96203641T DK0780469T3 (da) 1995-12-21 1996-12-19 Udødeliggjorte cellelinier stammende fra normalt menneskeligt hudvæv og serumfrit vækstmedium til dyrkning af sådanne

Country Status (23)

Country Link
US (2) US6423540B2 (da)
EP (2) EP0877797A1 (da)
JP (1) JP4404965B2 (da)
KR (1) KR100455006B1 (da)
CN (1) CN1154717C (da)
AT (1) ATE199390T1 (da)
AU (1) AU730222B2 (da)
BR (1) BR9612256B1 (da)
CA (1) CA2234118C (da)
CZ (1) CZ297289B6 (da)
DE (1) DE69611894T2 (da)
DK (1) DK0780469T3 (da)
ES (1) ES2155166T3 (da)
GR (1) GR3035719T3 (da)
HU (1) HU226195B1 (da)
IL (1) IL124191A (da)
NO (1) NO326190B1 (da)
NZ (1) NZ325814A (da)
PL (1) PL195108B1 (da)
PT (1) PT780469E (da)
RU (1) RU2215030C2 (da)
SK (1) SK283314B6 (da)
WO (1) WO1997023602A1 (da)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100455006B1 (ko) * 1995-12-21 2005-01-13 소시에떼 데 프로듀이 네슬레 소시에떼아노님 향상된불사화사람피부세포계및이의제조에유용한신규한무혈청배지
ES2226383T3 (es) * 1998-04-17 2005-03-16 Societe Des Produits Nestle S.A. Linea de celulas inmortalizadas derivadas de tejidos cutaneos humanos normales.
US6964869B2 (en) 1998-07-13 2005-11-15 Wisconsin Alumni Research Foundation Method and composition for skin grafts
CN1087778C (zh) * 1999-03-02 2002-07-17 华东理工大学 杂交瘤细胞无血清培养基
DE19912798C1 (de) * 1999-03-10 2000-02-17 Andreas Jordan Verfahren zur Kultivierung von Krebszellen aus Humangewebe und Vorrichtung zur Aufbereitung von Gewebeproben
AUPR298901A0 (en) 2001-02-07 2001-03-08 McComb Foundation, Inc., The Cell suspension preparation technique and device
ATE368856T1 (de) * 2001-04-24 2007-08-15 Wisconsin Alumni Res Found Verfahren und zusammensetzung für hauttransplantate
AU2002342613A1 (en) 2001-05-09 2002-11-25 Geron Corporation Treatment for wounds
US20030022369A1 (en) * 2001-05-18 2003-01-30 Helen Fillmore Differentiation of specialized dermal and epidermal cells into neuronal cells
EP1417629B1 (en) * 2001-07-06 2008-02-20 Lipomics Technologies, Inc. Generating, viewing, interpreting, and utilizing a quantitative database of metabolites
GB0208041D0 (en) 2002-04-08 2002-05-22 Lonza Biologics Plc Method of culturing animal cells
US20050025737A1 (en) * 2003-07-30 2005-02-03 Sebagh Jean Louis Compositions containing melon extracts
JP4532493B2 (ja) * 2003-09-18 2010-08-25 レイベン バイオテクノロジーズ，インコーポレイティド 細胞培養培地
WO2005071065A1 (en) * 2004-01-26 2005-08-04 Cognis France S.A.S Method for studying functional interactions between sensory neurons and keratinocytes or melanocytes
JP2008532550A (ja) 2005-03-17 2008-08-21 ストラタテック コーポレーション 純度が改良された代用皮膚
EP1818392B1 (en) * 2006-02-14 2010-08-25 Genetix Limited Cell culture medium
WO2010065907A2 (en) * 2008-12-05 2010-06-10 The United States Of America As Represented By The Secretary, Department Of Health And Human Services Use of a rock inhibitor to sustain primary human keratinocytes in a proliferative state
JPWO2010134619A1 (ja) * 2009-05-18 2012-11-12 国立大学法人東北大学 人工多能性幹細胞からの上皮系前駆細胞・幹細胞群及び角膜上皮細胞群の分化誘導方法
KR101768632B1 (ko) * 2011-05-18 2017-08-17 (주)아모레퍼시픽 칼슘 펄스를 통한 배양상피세포층의 분리방법
GB2497249B (en) 2011-07-12 2016-12-28 Foodchek Systems Inc Culture medium,method for culturing salmonella and e.coli and method for detecting salmonella and e.coli
WO2013129885A1 (ko) * 2012-02-28 2013-09-06 건국대학교 산학협력단 세포 배양액
US10335439B2 (en) 2013-03-11 2019-07-02 Jcr Pharmaceuticals Co., Ltd. Method for producing human corneal epithelium sheet
AU2013205148B2 (en) 2013-03-14 2014-10-30 AVITA Medical Americas, LLC Systems and methods for tissue processing and preparation of cell suspension therefrom
WO2016085304A1 (ko) * 2014-11-28 2016-06-02 (주)아모레퍼시픽 멜라닌 세포주 계대배양계 및 이를 이용한 노화 수준 판단, 미용 정보 제공, 또는 스크리닝 방법
CN107109901A (zh) * 2015-01-26 2017-08-29 哈利伯顿能源服务公司 旋转超硬切削元件
CN105238739A (zh) * 2015-11-11 2016-01-13 朱宁文 临床治疗级细胞治疗用黑色素细胞(melanocytes)规模化制备人类细胞外基质筛选培养方法
CN106520674A (zh) * 2016-12-24 2017-03-22 严志海 一种用于表皮黑素细胞体外培养的无血清培养基
CN107115219A (zh) * 2017-05-12 2017-09-01 青岛赛欧凯普图乐生物医药有限公司 一种纤维芽母细胞活化液面膜配方
CN114058565A (zh) * 2020-07-31 2022-02-18 上海尚瑞生物医药科技有限公司 一种无血清的黑素母细胞培养液及其培养方法
KR102583179B1 (ko) * 2020-11-27 2023-09-26 (주)엑셀세라퓨틱스 칼슘, 상피세포성장인자 및 알부민을 포함하는 각질형성세포 수립 또는 배양용 배지 조성물

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4940666A (en) * 1983-07-15 1990-07-10 University Patents, Inc. Process and defined medium for growth of human epidermal keratinocyte cells
US4885238A (en) 1987-10-30 1989-12-05 The United States Of America As Represented By The Department Of Health And Human Services Immortalized human bronchial epitherial mesothelial cell lines
US5292655A (en) * 1990-01-29 1994-03-08 Wille Jr John J Method for the formation of a histologically-complete skin substitute
US5376542A (en) 1992-04-27 1994-12-27 Georgetown University Method for producing immortalized cell lines using human papilluma virus genes
JPH10500669A (ja) * 1994-04-12 1998-01-20 アルザ・コーポレーション サイトカイン阻害薬に関するスクリーニング法
DE19617261A1 (de) * 1995-04-21 1996-11-28 Naeher Helmut Dr Med Priv Doz Hautverträglichkeitstest
KR100455006B1 (ko) * 1995-12-21 2005-01-13 소시에떼 데 프로듀이 네슬레 소시에떼아노님 향상된불사화사람피부세포계및이의제조에유용한신규한무혈청배지

Also Published As

Publication number Publication date
CA2234118A1 (en) 1997-07-03
AU1305497A (en) 1997-07-17
CN1205737A (zh) 1999-01-20
BR9612256B1 (pt) 2010-08-10
KR19990071817A (ko) 1999-09-27
EP0877797A1 (en) 1998-11-18
HUP9903742A2 (hu) 2000-03-28
US6949381B2 (en) 2005-09-27
ATE199390T1 (de) 2001-03-15
CZ194598A3 (cs) 1998-11-11
CA2234118C (en) 2009-04-07
JP4404965B2 (ja) 2010-01-27
PL195108B1 (pl) 2007-08-31
BR9612256A (pt) 1999-07-13
GR3035719T3 (en) 2001-07-31
CZ297289B6 (cs) 2006-10-11
AU730222B2 (en) 2001-03-01
HU226195B1 (hu) 2008-06-30
SK83598A3 (en) 1999-01-11
KR100455006B1 (ko) 2005-01-13
WO1997023602A1 (en) 1997-07-03
DE69611894T2 (de) 2001-09-13
JP2000506374A (ja) 2000-05-30
PL327320A1 (en) 1998-12-07
NZ325814A (en) 2000-10-27
NO982810D0 (no) 1998-06-18
IL124191A (en) 2004-06-01
DE69611894D1 (de) 2001-04-05
RU2215030C2 (ru) 2003-10-27
EP0780469B1 (fr) 2001-02-28
NO326190B1 (no) 2008-10-13
ES2155166T3 (es) 2001-05-01
CN1154717C (zh) 2004-06-23
US20020042129A1 (en) 2002-04-11
US6423540B2 (en) 2002-07-23
NO982810L (no) 1998-08-21
US20020012993A1 (en) 2002-01-31
HUP9903742A3 (en) 2000-10-30
EP0780469A1 (fr) 1997-06-25
PT780469E (pt) 2001-06-29
SK283314B6 (sk) 2003-05-02

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