CN113631980B - 共聚焦显微镜单元和共聚焦显微镜 - Google Patents

共聚焦显微镜单元和共聚焦显微镜 Download PDF

Info

Publication number
CN113631980B
CN113631980B CN202080024983.6A CN202080024983A CN113631980B CN 113631980 B CN113631980 B CN 113631980B CN 202080024983 A CN202080024983 A CN 202080024983A CN 113631980 B CN113631980 B CN 113631980B
Authority
CN
China
Prior art keywords
subunit
light
excitation light
mirror
confocal microscope
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202080024983.6A
Other languages
English (en)
Chinese (zh)
Other versions
CN113631980A (zh
Inventor
山下慈郎
田边康行
松田俊辅
寺田浩敏
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hamamatsu Photonics KK
Original Assignee
Hamamatsu Photonics KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hamamatsu Photonics KK filed Critical Hamamatsu Photonics KK
Publication of CN113631980A publication Critical patent/CN113631980A/zh
Application granted granted Critical
Publication of CN113631980B publication Critical patent/CN113631980B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0036—Scanning details, e.g. scanning stages
    • G02B21/0048—Scanning details, e.g. scanning stages scanning mirrors, e.g. rotating or galvanomirrors, MEMS mirrors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0064—Optical details of the image generation multi-spectral or wavelength-selective arrangements, e.g. wavelength fan-out, chromatic profiling
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/10—Beam splitting or combining systems
    • G02B27/14—Beam splitting or combining systems operating by reflection only
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B26/00—Optical devices or arrangements for the control of light using movable or deformable optical elements
    • G02B26/08—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light
    • G02B26/0816—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light by means of one or more reflecting elements
    • G02B26/0833—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light by means of one or more reflecting elements the reflecting element being a micromechanical device, e.g. a MEMS mirror, DMD
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/10—Beam splitting or combining systems
    • G02B27/14—Beam splitting or combining systems operating by reflection only
    • G02B27/145—Beam splitting or combining systems operating by reflection only having sequential partially reflecting surfaces

Landscapes

  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
CN202080024983.6A 2019-03-28 2020-03-26 共聚焦显微镜单元和共聚焦显微镜 Active CN113631980B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019-062976 2019-03-28
JP2019062976 2019-03-28
PCT/JP2020/013801 WO2020196784A1 (ja) 2019-03-28 2020-03-26 共焦点顕微鏡ユニット及び共焦点顕微鏡

Publications (2)

Publication Number Publication Date
CN113631980A CN113631980A (zh) 2021-11-09
CN113631980B true CN113631980B (zh) 2023-07-28

Family

ID=72608840

Family Applications (2)

Application Number Title Priority Date Filing Date
CN202080024983.6A Active CN113631980B (zh) 2019-03-28 2020-03-26 共聚焦显微镜单元和共聚焦显微镜
CN202080025057.0A Active CN113646686B (zh) 2019-03-28 2020-03-26 共聚焦显微镜单元和共聚焦显微镜

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN202080025057.0A Active CN113646686B (zh) 2019-03-28 2020-03-26 共聚焦显微镜单元和共聚焦显微镜

Country Status (5)

Country Link
US (2) US12117600B2 (enExample)
EP (2) EP3951466B1 (enExample)
JP (2) JP7344281B2 (enExample)
CN (2) CN113631980B (enExample)
WO (2) WO2020196783A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7534984B2 (ja) 2021-03-03 2024-08-15 株式会社日立ハイテク 分光測定装置
EP4296748A4 (en) * 2021-04-26 2025-03-05 Hamamatsu Photonics K.K. CONFOCAL MICROSCOPE UNIT, CONFOCAL MICROSCOPE AND CONTROL METHOD FOR CONFOCAL MICROSCOPE UNIT
US12189137B2 (en) * 2022-02-23 2025-01-07 The Boeing Company System and method for super-resolution imaging
DE102022114257A1 (de) * 2022-06-07 2023-12-07 Ludwig-Maximilians-Universität München (Körperschaft des öffentlichen Rechts) Baukastensystem für eine Mikroskopievorrichtung, Mikroskopievorrichtung und Verfahren zum Herstellen einer Mikroskopievorrichtung

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5535052A (en) * 1992-07-24 1996-07-09 Carl-Zeiss-Stiftung Laser microscope
JP3917731B2 (ja) 1996-11-21 2007-05-23 オリンパス株式会社 レーザ走査顕微鏡
JP2003185927A (ja) 2001-12-13 2003-07-03 Olympus Optical Co Ltd 走査型レーザー顕微鏡
US7038848B2 (en) 2002-12-27 2006-05-02 Olympus Corporation Confocal microscope
EP1688774B1 (en) 2003-11-26 2013-02-20 Olympus Corporation Laser scanning type fluorescent microscope
JP4409390B2 (ja) 2004-08-24 2010-02-03 オリンパス株式会社 光走査型共焦点観察装置
JP2006133499A (ja) 2004-11-05 2006-05-25 Shimadzu Corp 共焦点スキャナ及び共焦点顕微鏡
JP5058625B2 (ja) * 2007-02-19 2012-10-24 オリンパス株式会社 レーザ顕微鏡
EP2259125B1 (de) 2007-10-22 2017-07-26 Tecan Trading AG Laser Scanner-Gerät für Fluoreszenzmessungen
JP2009116082A (ja) 2007-11-07 2009-05-28 Nsk Ltd 光走査ユニット及び観察装置
JP2009198980A (ja) 2008-02-25 2009-09-03 Nikon Corp 共焦点顕微鏡
DE102008029458B4 (de) 2008-06-20 2019-02-07 Carl Zeiss Microscopy Gmbh Verfahren zum Aufzeichnen von Impulssignalen
JP5371694B2 (ja) 2009-10-26 2013-12-18 オリンパス株式会社 顕微鏡接続ユニットおよび顕微鏡システム
JP5452180B2 (ja) * 2009-11-13 2014-03-26 オリンパス株式会社 顕微鏡装置
JP5056871B2 (ja) 2010-03-02 2012-10-24 横河電機株式会社 共焦点顕微鏡システム
US20130015370A1 (en) * 2011-07-15 2013-01-17 Huron Technologies International In Confocal fluorescence slide scanner with parallel detection
JP5926966B2 (ja) 2012-01-30 2016-05-25 オリンパス株式会社 蛍光観察装置
JP5969803B2 (ja) 2012-04-23 2016-08-17 オリンパス株式会社 顕微鏡装置
DE102014017001A1 (de) 2014-11-12 2016-05-12 Carl Zeiss Ag Mikroskop mit geringem Verzeichnungsfehler
CN114002196B (zh) * 2016-02-22 2025-03-28 株式会社日立高新技术 发光检测装置
CN106980174B (zh) 2017-02-28 2019-04-16 浙江大学 一种综合性荧光超分辨显微成像装置

Also Published As

Publication number Publication date
CN113646686B (zh) 2023-10-03
JPWO2020196784A1 (enExample) 2020-10-01
US12117600B2 (en) 2024-10-15
EP3951468A1 (en) 2022-02-09
EP3951468B1 (en) 2025-05-07
JP7488253B2 (ja) 2024-05-21
JPWO2020196783A1 (enExample) 2020-10-01
US12078789B2 (en) 2024-09-03
US20220179185A1 (en) 2022-06-09
EP3951468A4 (en) 2023-01-04
WO2020196783A1 (ja) 2020-10-01
CN113631980A (zh) 2021-11-09
CN113646686A (zh) 2021-11-12
EP3951466B1 (en) 2025-05-07
EP3951466A4 (en) 2023-01-04
EP3951466A1 (en) 2022-02-09
JP7344281B2 (ja) 2023-09-13
WO2020196784A1 (ja) 2020-10-01
US20220155577A1 (en) 2022-05-19

Similar Documents

Publication Publication Date Title
CN113631980B (zh) 共聚焦显微镜单元和共聚焦显微镜
JP5289884B2 (ja) レーザ顕微鏡装置
US8873046B2 (en) Spectroscopic detection device and confocal microscope
US9389120B2 (en) Spectral detector or laser scanning microscope having variable filtration using spatial color separation
KR20100087141A (ko) 레이저 여기 형광 현미경
EP3098642A1 (en) Scanning optical system and scanning device
JP4434882B2 (ja) レーザ走査型蛍光観察装置
JP6659548B2 (ja) 多色走査型顕微鏡
JP2011118070A (ja) 共焦点走査型顕微鏡
JP2006030988A (ja) レーザ走査型顕微鏡
CN113631981B (zh) 扫描型显微镜单元
JP2004341204A (ja) 蛍光顕微鏡
JP4818634B2 (ja) 走査型蛍光観察装置
JP7351323B2 (ja) 共焦点スキャナ、共焦点スキャナシステム、及び共焦点顕微鏡システム
JP5321305B2 (ja) 走査型顕微鏡
JPH0829692A (ja) 蛍光顕微鏡
JP2011058953A (ja) 検出装置、それを備えた光学装置
JP2001117013A (ja) 共焦点レーザ顕微鏡
JP2006195390A (ja) レーザ走査型蛍光顕微鏡および検出光学系ユニット
JP2017181182A (ja) 分光測定装置
JP2008164719A (ja) 走査型共焦点顕微鏡
JP5136294B2 (ja) 共焦点顕微鏡

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant