CN112120012A - 一种car-t细胞冻存方法 - Google Patents
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- RVGRUAULSDPKGF-UHFFFAOYSA-N Poloxamer Chemical compound C1CO1.CC1CO1 RVGRUAULSDPKGF-UHFFFAOYSA-N 0.000 claims abstract description 28
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0278—Physical preservation processes
- A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
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Abstract
Description
实施例1 | 实施例2 | 实施例3 | 实施例4 | 对比例1 | 对比例2 | 对比例3 | 对照组 | 空白组 |
90.0±2.8 | 90.3±1.3 | 96.3±2.7 | 94.5±1.1 | 85.3±2.3 | 79.3±2.3 | 68.3±1.6 | 98.3±2.1 | - |
0h | 24h | 48h | 72h | |
实施例1 | 0.28±0.12 | 0.33±0.10 | 1.25±0.18 | 2.11±0.09 |
实施例2 | 0.30±0.09 | 0.36±0.16 | 1.23±0.15 | 2.06±0.08 |
实施例3 | 0.31±0.16 | 0.42±0.11 | 1.42±0.10 | 2.22±0.12 |
实施例4 | 0.27±0.13 | 0.29±0.14 | 1.11±0.13 | 2.09±0.10 |
对比例1 | 0.21±0.15 | 0.26±0.06 | 0.79±0.11 | 1.25±0.16 |
对比例2 | 0.20±0.17 | 0.26±0.08 | 0.67±0.12 | 1.25±0.17 |
对比例3 | 0.16±0.11 | 0.27±0.30 | 0.55±0.17 | 1.26±0.09 |
对照组 | 0.36±0.13 | 0.44±0.10 | 1.67±0.18 | 2.36±0.15 |
空白组 | - | - | - | - |
实施例1 | 实施例2 | 实施例3 | 实施例4 | 对比例1 | 对比例2 | 对比例3 | 对照组 |
40.3±1.2 | 40.0±1.4 | 43.3±1.0 | 39.8±1.5 | 34.2±1.1 | 32.3±1.0 | 26.3±1.7 | 44.3±2.0 |
Claims (9)
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