CN109705175B - 使用模拟移动床色谱纯化中性人乳寡糖的方法 - Google Patents

使用模拟移动床色谱纯化中性人乳寡糖的方法 Download PDF

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CN109705175B
CN109705175B CN201811603666.0A CN201811603666A CN109705175B CN 109705175 B CN109705175 B CN 109705175B CN 201811603666 A CN201811603666 A CN 201811603666A CN 109705175 B CN109705175 B CN 109705175B
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solution
human milk
lacto
neutral
chromatography
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CN109705175A (zh
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斯特凡·詹尼文
马库斯·海福力克
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Kohansen Breast Milk Oligosaccharides Co ltd
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Kohansen Hmo Co ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/15Vitamins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/40Complete food formulations for specific consumer groups or specific purposes, e.g. infant formula
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K31/70Carbohydrates; Sugars; Derivatives thereof
    • A61K31/702Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
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    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00Drugs for disorders of the alimentary tract or the digestive system
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/14Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/10Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1814Recycling of the fraction to be distributed
    • B01D15/1821Simulated moving beds
    • B01D15/185Simulated moving beds characterised by the components to be separated
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    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361Ion-exchange
    • B01D15/362Cation-exchange
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    • B01D61/00Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/02Reverse osmosis; Hyperfiltration ; Nanofiltration
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D61/00Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/42Electrodialysis; Electro-osmosis ; Electro-ultrafiltration; Membrane capacitive deionization
    • B01D61/422Electrodialysis
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
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    • C07H1/06Separation; Purification
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
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    • C07H1/08Separation; Purification from natural products
    • CCHEMISTRY; METALLURGY
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    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/06Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
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    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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CN201811603666.0A 2013-10-04 2014-10-02 使用模拟移动床色谱纯化中性人乳寡糖的方法 Active CN109705175B (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP13187369.7A EP2857410A1 (en) 2013-10-04 2013-10-04 Process for purification of 2´-fucosyllactose using simulated moving bed chromatography
EP13187369.7 2013-10-04
CN201480054230.4A CN105814070A (zh) 2013-10-04 2014-10-02 使用模拟移动床色谱纯化中性人乳寡糖的方法
PCT/EP2014/071145 WO2015049331A1 (en) 2013-10-04 2014-10-02 Process for purification of a neutral human milk oligosaccharide using simulated moving bed chromatography

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CN109705175B true CN109705175B (zh) 2022-07-05

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US (2) US10435427B2 (cg-RX-API-DMAC7.html)
EP (2) EP2857410A1 (cg-RX-API-DMAC7.html)
JP (1) JP6666243B2 (cg-RX-API-DMAC7.html)
KR (1) KR102298281B1 (cg-RX-API-DMAC7.html)
CN (2) CN109705175B (cg-RX-API-DMAC7.html)
BR (1) BR112016007342B1 (cg-RX-API-DMAC7.html)
DK (1) DK3063159T3 (cg-RX-API-DMAC7.html)
ES (1) ES2868249T3 (cg-RX-API-DMAC7.html)
MX (2) MX373919B (cg-RX-API-DMAC7.html)
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RU (1) RU2685537C2 (cg-RX-API-DMAC7.html)
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PL3494805T3 (pl) 2017-12-08 2025-10-06 Chr. Hansen HMO GmbH Suszone rozpyłowo tetrasacharydy
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KR20210006916A (ko) * 2018-05-07 2021-01-19 젠와인 바이오테크놀로지 게엠바하 미생물 발효에 의해 수득된 탄수화물로부터 락토-N-네오테트라오스 (LNnT)를 정제하기 위한 간단한 방법
US20210230656A1 (en) * 2018-05-23 2021-07-29 Dsm Ip Assets B.V. Production of oligosaccharides
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CN113260624A (zh) 2018-10-18 2021-08-13 巴斯夫欧洲公司 2′-o-岩藻糖基乳糖的晶型ii、其制备方法、包含该物质的营养、化妆品和药物配制物
EP3741770A1 (en) * 2019-05-21 2020-11-25 Jennewein Biotechnologie GmbH Purification of oligosaccharides from a fermentation broth by using filtration
EP3750902A1 (en) * 2019-06-14 2020-12-16 Jennewein Biotechnologie GmbH Process for the purification of lacto-n-neotetraose
WO2021011905A1 (en) * 2019-07-18 2021-01-21 The Regents Of The University Of California Human milk oligosaccharides for control of dietary response and metabolic phenotype
CN114364389A (zh) 2019-09-24 2022-04-15 普罗莱克塔生物科学公司 用于治疗炎性和免疫疾病的组合物和方法
CN110672756B (zh) * 2019-11-07 2020-12-29 江南大学 一种奶粉中2’-岩藻糖基乳糖含量的检测方法
EP4097118A4 (en) * 2020-01-29 2024-02-28 DSM IP Assets B.V. METHOD FOR OBTAINING AND PURIFYING OLIGOSACCHARIDES FROM HUMAN MILK
WO2021186258A1 (en) * 2020-03-20 2021-09-23 Glycom A/S Methods for providing a solid hmo product, and solid hmo products obtained thereby
CN116075517A (zh) * 2020-06-12 2023-05-05 巴斯夫欧洲公司 改进的发酵液的脱矿化与诸如寡糖的精细化学品的纯化
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CN112526022A (zh) * 2020-11-27 2021-03-19 内蒙古伊利实业集团股份有限公司 一种乳中母乳低聚糖的检测方法
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CN117003803A (zh) 2022-05-07 2023-11-07 山东恒鲁生物科技有限公司 一种三糖的新晶型
CN115873051B (zh) 2022-05-17 2024-06-25 山东恒鲁生物科技有限公司 三糖的新晶型
CN115073539B (zh) * 2022-07-21 2023-06-16 南京工业大学 一种2’-岩藻糖基乳糖分离纯化的方法
CN115260255B (zh) * 2022-08-30 2024-06-28 南京工业大学 一种利用模拟移动床分离纯化2’-岩藻糖基乳糖的方法
WO2024130119A2 (en) 2022-12-16 2024-06-20 Prolacta Bioscience, Inc. Synbiotic compositions for short chain fatty acid production
CN115856151B (zh) * 2022-12-27 2025-08-12 深圳市中科朗健生物技术有限公司 一种同时测定多种人乳低聚糖的高效液相色谱检测方法
CN115838390A (zh) * 2022-12-30 2023-03-24 保龄宝生物股份有限公司 一种利用三组分色谱分离技术制备高含量2′-岩藻糖基乳糖的方法
WO2024165630A1 (en) * 2023-02-07 2024-08-15 N.V. Nutricia Nutritional composition with improved segregation resistance
WO2024218197A1 (en) * 2023-04-19 2024-10-24 Chr. Hansen A/S Human milk oligosaccharides
WO2025056571A1 (en) 2023-09-12 2025-03-20 Frieslandcampina Nederland B.V. Milk extracellular vesicle enrichment process
CN117089503B (zh) 2023-10-17 2024-01-02 保龄宝生物股份有限公司 一种大肠杆菌k-12 mg1655 blbyzt6及其应用
WO2025149372A1 (en) 2024-01-09 2025-07-17 Frieslandcampina Nederland B.V. Milk extracellular vesicle enrichment process using peg
CN119192253B (zh) * 2024-09-06 2025-10-21 虹摹生物科技(上海)有限公司 一种岩藻糖基乳糖及其纯化方法
CN120847064B (zh) * 2025-09-23 2025-12-05 吉林省科技创新研究院有限公司 一种液体中微量可见异物分析鉴定方法

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