CN107996166A - Chinese catalpa rapid cuttage mating system - Google Patents

Chinese catalpa rapid cuttage mating system Download PDF

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Publication number
CN107996166A
CN107996166A CN201711395230.2A CN201711395230A CN107996166A CN 107996166 A CN107996166 A CN 107996166A CN 201711395230 A CN201711395230 A CN 201711395230A CN 107996166 A CN107996166 A CN 107996166A
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cuttage
chinese catalpa
cuttings
disinfection
mating system
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CN107996166B (en
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鲁仪增
李文清
刘启虎
杨海平
刘立江
庄振杰
孙涛
韩义
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CENTER FOR FOREST GENETIC RESOURCES OF SHANDONG PROVINCE
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics

Abstract

The present invention relates to asexual multiplication seedling technical field, especially Chinese catalpa rapid cuttage mating system, include the following steps:Chinese catalpa kind, clonal annual healthy and strong kind bar are selected, disinfection, puts and cuttings is cultivated in germination accelerating pond, and coppice shoot is obtained after 5~7 weeks, and trimming obtains the laggard disinfection of cuttings;Select peat:Perlite=2:1 is used as cutting medium, loads hole tray, with 1L talcum powder, mixes No. ABT1 500 × 10‑6No. mg/L+GGR7 80 × 10‑6Pulvis of taking root is made in mg/L, and cuttings base portion uniformly dips root-inducing powder pulvis, the hole being put into hole tray, and clear water fills saturating after fixing, and sprays microbial inoculum;The atomization management of 1~10d after cuttage, stops atomization after 10d, increases illumination, ventilation, periodically sprays fungicide, cuttage periodically sprays foliar fertilizer after 3 weeks, obtains cuttage seeding;After cuttage 5 weeks, abundant illumination, temperature control, regular watering, fertilising, carry out hardening;During cuttage 7~8 weeks, transplanting, flood irrigates, after watered, the management such as apply fertilizer.The present invention has rooting rate high, and versatility is good, the low beneficial effect of cost.

Description

Chinese catalpa rapid cuttage mating system
Technical field
The present invention relates to asexual multiplication seedling technical field, especially Chinese catalpa rapid cuttage mating system.
Background technology
Chinese catalpa (Catalpa bungei C.A.Mey) belongs to (Catalpa Scop.) for Bignoniaceae (Bignoniaceae) Chinese catalpa Tall and big deciduous tree, it has a very wide distribution, union high quality timber, ecological protection, rare ornamental plantation, improvement atmospheric environment and medicine With multiple functions such as health cares in one, it is the distinctive Precious Timber Species in China and ornamental tree species, is known as the laudatory title of " wooden king ", have There is the cultivation history (Guo Congjian etc., 1988) of more than 2100 years.Since " six or five ", Chinese scholar starts system to Chinese catalpa platymiscium and grinds Study carefully, so far the whole nation using traditional local varieties or superior type more than 20 (Pan Qingkai etc., 1991), selection and breeding correlation new varieties, Breeding more than 50, up to a hundred of superior hybrid clone, popularizing area mus up to a million, beautify, manually for China's green barren hill, cities and towns Timber stands construction, restoration of the ecosystem etc. are made that particularly important contribution.Asexual multiplication seedling method is to obtain Chinese catalpa breeding strong sprout Best mode, it is stock that its asexual reproduction method, which mainly has root segment cuttage (burying root nursery) method, Chinese catalpa (C.ovata G.Don), 4 kinds of grafting, cottage method and tissue culture method etc..Some scholars also inquired into Chinese catalpa Embryogenic Callus Suspension Culture foundation and Plant regeneration, but the technology is not yet applied in production.
Chinese catalpa belongs to cuttage seeds difficult to take root, and self-sterility for it, seldom solid, and has Seedling propagation is difficult to be sent out with seed The problems such as bud rate is low, root segment cuttage (burying root nursery) mode nursery is from the cuttage side that the 1980s continues to use always so far Formula, it is of a relatively high to bury root propagation survival rate, different clones root segment cuttage emergence rate 77~95%, and bed is covered with plastics Small plastic shed Face can also improve emergence rate;But during using root segment cuttage mode nursery, there are its root segment limited amount, length and thickness to fit When root segment it is difficult obtain, and influenced during obtaining by surrounding enviroment or facility, easily resource and surrounding damaged, taken a lot of work The problem of time-consuming.During root segment cuttage (burying root nursery), nursery stock germination is unearthed evening, irregular, and production seedling amount is low or cannot go out garden then.
When breeding Chinese catalpa breeding using grafting method, due to Chinese catalpa, self-sterility, seldom solid, and has Seedling propagation to be stranded The difficult problem such as low with percentage of seedgermination, Chinese catalpa stock is few, and often selection Chinese catalpa seedling is up to as stock, its root-knot nematode infection rate 100%th, the problems such as often resulting in flexible serious root-rot, dry type, growth potential decline;Since stock and Growth status speed differ Cause, easily occur upper coarse and lower fine " bound feet phenomenon ".Although grafting professional standard is formulated《Precious timber cultivation technique is advised Journey Chinese catalpa (LY T 2125~2013)》, but Chinese catalpa breeding own-rooted tree is the direction of Chinese catalpa development.
Cutting propagation technology is that the ideal method of catalpa bungei vegetative propagation nursery, especially epicormic branch cuttage are Chinese catalpa fast seedling growings In important technology.It is numerous that the multidigit such as Chen Wei scholar has inquired into three kinds of root segment cuttage (burying root nursery), epicormic branch cuttage, grafting etc. in detail Mode is grown, research shows, cuttage seeding growing way is significantly better than other two modes of reproduction.Chinese catalpa breeding seedling is bred using cuttage technique Wood, becomes the raising technology that scholar in industry, nursery practitioner etc. are most interested and development correlative study in the past 10 years is most One of.Since Chinese catalpa cuttage root-taking is difficult, common cuttage technique survival rate is very low always, it belongs to cuttage seeds difficult to take root, Chinese catalpa Set breeding nursery stock demand to mismatch with its raising technology capability development, seriously constrain the large-scale production of Chinese catalpa breeding.
To solve Chinese catalpa cuttage raise seedling technique problem, by tens experts and scholars' researchs in more than 30 years, tentatively solves portion Graded kind, clonal cutting propagation technical barrier, recent years, part experts and scholars also declared related patents technology successively. But still suffer from some problems, most improved seeds, clonal cutting propagation skill in technology versatility, repeatability etc. Art not yet solves.But intelligent tinkling of pieces of jade of , YUN etc. (2007) research explanation, the skewer between different clones, different familys, different type cuttings Slotting rooting rate is taken root best (rooting rate with 9-1 there are larger difference, Chinese catalpa different cultivars are different to the requirement taken root 86.7%), secondly leaflet spun gold Chinese catalpa (rooting rate 86.4%), the clone 9-1 of rooting rate more than 70%>Leaflet spun gold>1-1 >2-7>013-1>2-2>2-1>008-1>2-8, the clone 015-1 between rooting rate 40~70%>2-6>Light leaf Chinese catalpa>1-2> 004-1>9-2>01, clone 001-1 of the rooting rate below 40%>1-4>002-1>02>011-1>1-3>6523>Great Ye spun golds >080, ash 3,038, Lip river ash and 4 clones such as line ash only have callus, difficult long root.Wang Shuncai (2007), Hao Mingzhuo etc. (2007), Wang Lianggui etc. (2008), beam have the research such as prosperous etc. (2008) Chinese catalpa all-optical cutting to also indicate that, Chinese catalpa kind or class Type is different, such as the long fruit Chinese catalpa of spun gold Qiu Yu gardens base, light leaf Chinese catalpa, Henan Chinese catalpa No. 1, all Chinese catalpas No. 2, all Chinese catalpas No. 3, Nanyang Chinese catalpa, Henan Chinese catalpa No. 2, ash The condition difference of taking root of 9 kinds such as Chinese catalpa, Chinese catalpa life, its required condition of taking root is different, in the actual production process, it is necessary to pin To different type (kind), it is all energy of taking root to find out each suitable Cutting conditions Nanyang Chinese catalpa, the long fruit Chinese catalpa of physa and Henan Chinese catalpa No. 1 The preferable kind of power (type);Hormone, spray position and cuttage period are very big to Rooting effect, choose spray taper in 6 months Ten days cuttage, rooting rate is preferable, and the proportioning processing of IBAl000+NAA500mg/kg is better than the effect of single hormone:Orthogonal test Show, the optimum combination of the long fruit Chinese catalpa cuttage of physa is the ABT6 root-inducing powders processing 30s with 1500mg/kg, cutting with 10~ 12cm long, 0.3~0.6cm are slightly to be excellent;For the optimum combination of spun gold Chinese catalpa cuttage is compared with the long fruit Chinese catalpa of physa, concentration and time become respectively For with the ABT6 root-inducing powders of 2000mg/kg and processing 2min;The optimum combination of light leaf Chinese catalpa cuttage is only then 2000mg/kg ABTl root-inducing powders;The IBA of 1500mg/kg is selected in No. 1 cuttage of Henan Chinese catalpa.Therefore, the related cuttage study condition of spun gold Chinese catalpa, not Suitable for most Chinese catalpa types (kind).
All right (2008) and (2010) two Master degree candidates of Zhang Bo, in the case where same tutor instructs successively in same intelligent temperature Room, using identical 5 Chinese catalpa kinds (the long fruit Chinese catalpa of physa, Henan Chinese catalpa No. 1, Henan Chinese catalpa No. 2, all Chinese catalpas No. 2, all Chinese catalpas No. 3) carried out Chinese catalpa Set softwood cutting propagation technical research;Under same auto-control pattern, Zhang Bo in the hormone kind all so found out and its Concentration (No. ABT1, NAA equal 1000 × 10-6G/L talcum powder is quickly dipped), matrix formulations (peat:Perlite=1:1) Primary Study such as As a result it is optimized on the basis of (rooting rate respectively reaches 65.6% and 67.64%), and thinks that No. ABT1 is optimal hormone, In the case where concentration and matrix are constant, rooting rate is 76~81.29, and base long fruit Chinese catalpa rooting rate in garden preferably up to 83%, is transplanted Survival rate 95.93%;When adopting fringe epicormic branch cuttage from outdoor cutting orchard, take optimal site cuttings, its cutting plantation for 52~ 70%;Its cuttage survival rate, transplanting survival rate are below state of the art.
Progress epicormic branch cuttage research shows after Zhao Kun etc. (2010) handles 10 clones using GGR, and it is asexual to influence Chinese catalpa Be tloig-cutlage reproductive effect factor it is very much, there were significant differences for the cutting plantation of different clones fringe bar.Participate in the experiment clone Tloig-cutlage rooting rate luffing is 47.8~91.1%.It is right in warm canopy after square qin etc. (2011) utilizes GGR6 HORMONE TREATMENTs 8611 and 4002 improved seeds carry out cuttage, its cuttage survival rate respectively reaches 76% and 80%.Liu Xiaojun (2016) Nian Wen Under room environmental, hold matrix with carton and inquired into relative influence of the different cuttings processing modes to Chinese catalpa cuttage cuttings, its During to off-test (45d), not yet take root.
Comprehensive explanation above, the subjectivities such as administrative skill are caused since the facilities and equipment provisioned in each experts and scholars is relatively simple and crude Factor is too strong and the difference or other factors of used Chinese catalpa breeding material, in turn results in technology used by their Scheme " having a strong temperament ", " repeatability " are weak.By the end of current, most kinds of Chinese catalpa, clone cutting propagation technology are not yet Solve, Chinese catalpa cuttage root-taking problem is still worth research, is still the heat in current Chinese catalpa clones forestry key technology research Point and difficult point.With the developing of modern facilities equipment and its management system, gradually popularize, relevant cost quickly reduces, in phase Under the conditions of answering facilities and equipment, standardization, be factory produced and easily realize.
The content of the invention
The present invention provides a kind of efficient Chinese catalpa rapid cuttage mating system, its whole cutting propagation process is standardizing General purpose steel-plastic/profiled bar glass structure greenhouse in carry out, it is internal have 1 cultivation hardening space (referred to as " production district ") and The insulating space (referred to as " cutting plot ") of the relatively independent control of at least one, production district and cutting plot should all have relatively independent Temperature control, interior external sunshade control light, skylight and side window ventilation, spray basic function, cutting plot should also have certain atomization facility.Solution Certainly Chinese catalpa is wide in variety in the prior art, and rooting rate is low, and clone softwood cutting propagation is difficult, technology repeatability and poor universality, It is cumbersome, the technical problem such as cost height.
To achieve these goals, the present invention adopts the following technical scheme that:
Chinese catalpa rapid cuttage mating system, carried out in general purpose steel-plastic/profiled bar glass structure greenhouse of standardization, it is special Sign is, comprises the following steps:
(1) bar selection is planted
Select Chinese catalpa kind, clonal annual healthy and strong kind bar;
(2) cuttings is cultivated
Carry out in cutting plot or production district, warm 25 DEG C of indoor temperature, humidity is not less than 70%;Selection is about 1~1.2m, slightly Hold diameter≤4cm, the healthy and strong kind bar disinfection of the no disease and pests harm of taper end diameter >=0.5cm, the horizontal positioned vernalization for spreading river sand inside Vernalization trunk is used as in pond, every kind of 1~2cm of stripe pitch, the river sand of kind bar upper strata covering 2cm thickness, periodically sprays efficient germicide, Maintain warm 25 DEG C of indoor temperature, humidity to be not less than 70%, cultivate 5~7 weeks and obtain coppice shoot;
(3) cuttings is handled
After the up to 7cm of coppice shoot 40% that step (2) obtains, retain whole blades, with sterilized cutter close to rudiment base It expands tissue and is removed together from vernalization trunk portion's band, and each coppice shoot length is preferred with 7~9cm, long as a cuttings Coppice shoot be cut into the cuttings of two sections of 5~6cm long with the secateurs after disinfection, cut off all cuttings base portions incidentally long bast Tissue, retains cuttings base portion length and width and is preferred no more than 0.5cm, and when obtaining 30~40 cuttings every time, carry out disinfection processing at once;
(4) cuttage
Carry out in cutting plot.Select peat:Perlite=2:1 is used as cutting medium, loaded in the hole tray after disinfection, and makes Standby root-inducing powder, the root-inducing powder are to replace water with talcum powder, mix No. ABT1 500 × 10-6No. mg/L+GGR7 80 × 10-6mg/L Manufactured pulvis;
It is 2.5cm to make a call to a depth in the middle part of each cuttage position in hole tray, the hole of a diameter of 1cm, by obtained by step (3) Cuttings base portion uniformly dip prepared root-inducing powder pulvis, the hole being put into hole tray, fixes cuttings, and saturating base is filled with clear water Matter, sprays fungicide;It is 98% that the atomization humidity in greenhouse is kept during cuttage, and temperature is 22~25 DEG C;
(5) managed after cuttage
Atomising regime is carried out in 1-10d after cuttage, stops atomization after 10d, increases illumination, be aerated, it is during which regular Fungicide is sprayed, cuttage can periodically spray foliar fertilizer after 3 weeks, obtain cuttage seeding;
(6) acclimatization and transplants
In cutting plot hardening, also can be in production district hardening.After cuttage 5 weeks, whole sunshade nets, greenhouse temperature control are opened daily For system at 28 DEG C, regular watering, fertilising, carry out hardening;During cuttage 7~8 weeks, portable cuttage seeding base portion, matrix is fully tied with root system Close, can be transplanted, 3d does not water before transplanting, goes out earing from hole tray, is put into togerther together with matrix in strong seedling cultivation garden Planting pit in, insert topsoil, after cuttings is fixed, flood irrigates, after watered, management, Qiu Mo or the second year such as apply fertilizer Spring goes out garden.
Increase root-inducing powder contact area, hestening rooting.If do not trimmed, first, causing cuttage difficult, second, because excessive attached Belong to tissue, cause to rot, influence to take root;Atomising regime mainly solves the problems, such as cuttage cuttings wilting, improves cuttage root-taking survival rate. The normal moisture that humidity is big, and blade can be kept to need is atomized, blade kept photosynthesis as far as possible, wilted, promotes cuttings Hormone in vivo synthesizes, hestening rooting.Once wilting, new bud, which emerges and grows blade, could exercise normal function, be given birth to extending Root time, base portion easily rot;By the hardening phase, cuttage seedling rooting, growth is vigorous, average cuttage survival rate up to 98% with On.
As further preferably, the incubation step of the annual strong non-hibernating eggs bar is as follows:Selection raw plan breeding in 1~2 year Kind, clone nursery stock, in more than butt stump promotees to sprout at 6~9cm, during rudiment up to 10~15cm, select and remain base portion and sprout It is raw, be evenly distributed in base portion surrounding, the coppice shoot of robust growth 2~3, remove unnecessary coppice shoot 2~3 times, late October wipes out the tip First 5~10cm, obtains Chinese catalpa kind, clonal annual healthy and strong kind bar.
As further preferably, the river sand water content 75% in the germination accelerating pond, river sand forms sand bed, sand bed temperature 15 DEG C~20 DEG C.
As further preferably, the healthy and strong bar of planting of the step (2) is with 300 × 10-6(50% is wettable for the carbendazim of mg/L Property pulvis) solution disinfection 35min;The cuttings disinfection way of the step (3) is to place vertically or slightly slant setting is 300 × 10- 615min is sterilized in carbendazim (50% wettable powder) solution of mg/L.
As further preferably, the river sand is with 500 × 10-6The potassium permanganate irrigation and disinfection of mg/L;The hole tray is used 1000×10-6The potassium permanganate irrigation and disinfection of mg/L.
As further preferably, the mist pipe step is as follows:After cuttage, preceding 5d, the atomization humidity in greenhouse is 98%, Temperature is 22~25 DEG C, and external sunshade is carried out with 70% sunshade net;During 6~10d, atomization humidity is progressively reduced extremely by 98% 75%, temperature is 25~28 DEG C, increasing illumination by way of opening the external sunshade net of total aperture 10%.
As further preferably, after stopping atomization plant, daily daytime regards weather conditions artificial watering 4~6 times, greenhouse Temperature should keep 25 DEG C~30 DEG C, and 10:00~14:00 opens side window or skylight (open overall aperture 20%) ventilation;Open The external sunshade net of total aperture 40~50%, increases illumination.
As further preferably, the sterilization method of the cutter and the secateurs is after soaking 35min with 75% alcohol Dry.
As further preferably, the fungicide that the step (4) sprays is 500 × 10-6(50% can for the carbendazim of mg/L Wet powder);It is that every 7d is sprayed one time 500 × 10 that the step (5), which periodically sprays fungicide,-6Mg/L first frosts MnZn (72% Wettable powder) and 2500 × 10-6Mg/L hymexazos (green prosperous No. 1), it is described periodically to spray foliar fertilizer, used per 5d with 0.3% Urea and 0.5% potassium dihydrogen phosphate mixed solution spray once, the step (5) further includes removal and goes mouldy cuttings with 800 ×10-6The potassium permanganate of mg/L pours the hole tray hole gone mouldy and its matrix.
As further preferably, step (6) 5~7d of watering time interval, per 7d spray 0.2% urea and 0.2% potassium dihydrogen phosphate mixed solution.
The beneficial effects of the invention are as follows:In the case where standardizing greenhouse experiment, 5 early stages of more difficult cutting propagation in Chinese catalpa are selected The kind educated and the choiceness of 4 selection and breeding recently, select cuttage breeding method provided by the invention, carry out Cutting test, and 9 The rooting rate of a kind is between 90.3~100%, and for average rooting rate up to 95%, rooting rate averagely improves more than 20%;After hardening Transplant survival is high, can be transplanted after 56d, and transplanting survival rate transplants no dead seedling, without slow seedling up to 100%, and seedling is averaged ground diameter then 1.5~3.2cm, average height of seedling 1.5m or so, can go out garden then.
Brief description of the drawings
Fig. 1 is the Chinese catalpa kind bar cultivation figure of the present invention;
Fig. 2 a are Chinese catalpa kind bar disinfection figures;
Fig. 2 b are Chinese catalpa kind bar embedment germination accelerating pond figures;
Fig. 2 c are Chinese catalpa cuttings figures;
Fig. 3 a are to cut heading figure;
Fig. 3 b are to cut cuttings of the base portion with bast;
Fig. 3 c are to dip Chinese catalpa cuttage figure after root-inducing powder;
Fig. 4 is Chinese catalpa cutting survival figure;
Fig. 5 is state diagram before transplanting after cuttage seeding hardening;
Fig. 6 is transplant survival figure.
Specific embodiment
The embodiment of the present invention is described below, it is clear that described embodiment is only part of the embodiment of the present invention, Instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not making creative labor All other embodiments obtained under the premise of dynamic, belong to the scope of protection of the invention.
Embodiment 1
Whole cutting propagation process carries out in the general purpose steel-plastic of standardization/profiled bar glass structure greenhouse, its internal tool There are space (referred to as " production district ") and the insulating space (referred to as " cuttage of the relatively independent control of at least one of 1 cultivation hardening Area "), production district and cutting plot should all have relatively independent temperature control, interior external sunshade control light, skylight and side window ventilation, spray base This function, cutting plot should also have certain atomization facility.
Chinese catalpa rapid cuttage mating system, step are as follows:
(1) bar is planted to cultivate
Selection 1 year raw plan breeding kind, clone nursery stock, stump promotees to sprout at butt above 8cm, and rudiment is up to During 12cm, select and remain base portion germinating and be evenly distributed in base portion surrounding, the coppice shoot of robust growth 2, remove unnecessary coppice shoot 3 times, 10 The last ten-days period moon wipe out tip of a branch 8cm, promote lower part sprout full, Chinese catalpa kind, clonal annual healthy and strong kind bar are cultivated, refering to figure 1。
(2) cuttings is cultivated
Refering to Fig. 2 a, Fig. 2 b, Fig. 2 c, Chinese catalpa cuttings cultivation is carried out.
17cm is placed with 500 × 10 in bottom in germination accelerating pond-6The river sand of the potassium permanganate irrigation and disinfection of mg/L, river sand composition Sand bed.
By be about 1.2m, butt diameter 3cm, taper end diameter 2cm no disease and pests harm healthy and strong kind bar with 300 × 10-6mg/L Carbendazim (50% wettable powder) solution disinfection 35min, refering to Fig. 2 a.
Refering to Fig. 2 b, then the horizontal positioned mode of kind bar after disinfection is embedded in germination accelerating pond, plants stripe pitch 1.5cm, Upper strata covering 2cm after it is sterilized after river sand.Keep river sand water content 75% or so, 15 DEG C~20 DEG C of sand bed temperature, greenhouse Interior 25 DEG C of temperature, humidity are not less than 70%.
Alternately sprayed one time 450 × 10 per 7d-6The first frost MnZn (72% wettable powder) of mg/L and 3000 × 10- 6Mg/L hymexazos (green prosperous No. 1).After 6 weeks, refering to Fig. 2 c, cuttings is obtained, starts epicormic branch cutting.
(3) cutting medium and hormone prescription
Peat:Perlite=2:1 is used as cutting medium, (depth about 5cm) is installed in 32 hole disks, with 1000 × 10- 6The potassium permanganate irrigation and disinfection of mg/L.Before cuttage, matrix is irrigated with clear water and is fully rinsed, avoids potassium permanganate from remaining.
Water is replaced with talcum powder, i.e., replaces 1L water with 1L talcum powder, is made into No. ABT1 500 × 10-6No. mg/L+GGR7 80 × 10-6The pulvis of mg/L, is put into brown bottle, and closed, lucifuge, Cord blood, are used while allocating.
(4) cuttings processing and cuttage
Refering to Fig. 3 a, Fig. 3 b, Fig. 3 c, cuttings processing and cuttage.Step (2) obtains coppice shoot, when 40% coppice shoot reaches 7cm Afterwards, the wallpaper knife dried after soaking 35min with 75% alcohol, refering to Fig. 3 a, pasting coppice shoot base portion band, it expands tissue from vernalization tree To remove on dry, be put into after taking in keg together, point number of being obtains, when obtaining 32 coppice shoots every time, Immediate management, cuttage.
Each cutting is 1 cuttings, retains whole blades, its length 7cm.Dried after soaking 35min with 75% alcohol Secateurs, long coppice shoot is cut into two 6cm cuttings;It is with secateurs after disinfection that cuttings base portion is subsidiary long refering to Fig. 3 b Phloem tissue is cut off, and is retained base portion length and width and is no more than 0.5cm.
Then cuttings is placed vertically or slightly slant setting is 300 × 10-6The carbendazim (50% wettable powder) of mg/L is molten 15min is sterilized in liquid.
During cuttage, with the thick diameter 1cm wooden sticks after disinfection, the matrix in the middle part of each cuttage position sterilized in hole tray Upper punching downwards, depth about 2.5cm.Cuttings base portion is dipped into root-inducing powder pulvis, cuttings base portion is all covered by root-inducing powder pulvis Lid, then put it into hole, finally with hand by cuttings surrounding matrix flicking to cuttings, make cuttings surrounding, base portion and matrix tight Contiguity is touched.After cuttage, saturating matrix is filled with clear water, then with 500 × 10-6Carbendazim (50% wettable powder) solution spray of mg/L Apply once.
It is 98% that the atomization humidity in greenhouse is kept during cuttage, and temperature is 22~25 DEG C.
(5) managed after cuttage
After cuttage, preceding 5d, greenhouse atomization humidity is maintained at 98%, and temperature is maintained at 22~25 DEG C, and external sunshade is hidden with 70% Screened postive covers;The atomization humidity in greenhouse is gradually reduced to 75% in subsequent 5d, greenhouse temperature should keep 25~28 DEG C, open total The external sunshade net of aperture 10%, increases illumination;After 10d, stop atomization plant, regard weather conditions artificial watering 6 daily daytime Secondary, greenhouse temperature should keep 25~30 DEG C, and 10:00~14:00 opens side window or skylight (open overall aperture 20%) ventilation; The external sunshade net of total aperture 50% is opened, increases illumination.
Treat that matrix somewhat becomes dry, when hole tray integrally lightens, holds matrix without dripping, pour it is permeable once.General each 3~5d Pour once.Alternately sprayed one time 500 × 10 per 7d-6The first frost MnZn (72% wettable powder) of mg/L and 2500 × 10- 6Mg/L hymexazos (green prosperous No. 1);Removal is gone mouldy cuttings and with 800 × 10-6The potassium permanganate of mg/L pours the cave gone mouldy Disk hole and its matrix.Cuttage starts to spray foliar fertilizer after 3 weeks, is mixed per 5d with 0.3% urea and 0.5% potassium dihydrogen phosphate Solution sprays once.
(6) acclimatization and transplants
After cuttage after 5 weeks, daily can fully open sunshade net, increase illumination, improve indoor temperature to 28 DEG C, promote nursery stock Take root, grow.By watering time interval to 5~7d, sprayed per 7d 0.2% urea and the mixing of 0.2% potassium dihydrogen phosphate it is molten Liquid.At 7~8 weeks, cuttage seeding base portion please be carry with hand, matrix is fully combined with root system, can integrally depart from hole tray hole, refering to Fig. 5, this When can transplant, 3d does not rewater before transplanting.
During transplanting, by cuttage from hole tray go out, be put into togerther in the planting pit in strong seedling cultivation garden, insert together with matrix Topsoil, makes root system combine closely with soil, and flood irrigates.
After cultivation in 2 weeks, watering, weeding soil moisture conservation are paid attention to;Survival rate, survival rate 100%, refering to Fig. 6 are investigated after 2 weeks.With Enter normal management afterwards, seedling is averaged ground diameter 2.8cm (1.5~3.2cm), average height of seedling 1.5m, Qiu Mo or second year spring then Garden can be gone out.
Culture substrate choice experiment
According to matrix peat:Perlite=1:1 (PP11), peat:Perlite=2:1 (PP21), hormone No. ABT1 500 × 10-6Mg/L (H500), No. ABT1 500 × 10-6No. mg/L+GGR7 80 × 10-6Mg/L (H580), which is designed, to be optimized, and is made The cuttage kind for selecting it to study:No. 2 long fruit Chinese catalpa of garden base, all Chinese catalpas No. 2, all Chinese catalpas No. 3, Henan Chinese catalpa No. 1 and Henan Chinese catalpa progress Cutting tests, This 5 kinds are the kind of early stage selection and breeding, each each 350 plants of cuttage of processing of system number, best to related kind in the prior art Rooting rate be compared, experimental result is shown in Table 1.
15 early stage Cultivars cutting plantation situations of table
Cutting test is carried out using PP11+H500, PP21+H500, PP21+H580 combination formula, result of the test is shown, excellent Matrix formulations and HORMONE TREATMENT after change, can improve more than 10% cutting plantation, and breach all No. 3 difficulties of taking root of Chinese catalpa The problem of.
PP11+H500 result of the tests show that hormone concentration is excessive in document, inhibits cuttage rooting.
PP11+H500, PP21+H500 result of the test show that matrix increase perlite ratio, helps to improve rooting rate, Due to perlite increase, matrix gas permeability and water penetration are increased, promotes and takes root.
PP21+H500, PP21+H580 result of the test are shown, adjust hormone prescription, 5 Chinese catalpas are improved in equal certain amplitude The rooting rate of kind is set, for rooting rate up to more than 90.3~96.6%, average rooting rate is 93.7%.
The versatility experiment of Chinese catalpa rapid cuttage mating system
By the four strains of Chinese catalpa:22-03,8-11,2-8,9-1 are carried out according to the cuttage breeding method described in embodiment 1 Cuttage is tested, and two clones of wherein 22-03 and 8-11 have no that Cutting test is reported, two many scholars of clone of 2-8 and 9-1 Carry out correlative study, its cutting plantation is up to 70.6% and 86.7% intelligent tinkling of pieces of jade of (YUN etc., and 2007), four strains are taken root Rate is shown in Table 2.
The choiceness cuttage root-taking situation of 24 selection and breeding recently of table
As it can be seen that the Chinese catalpa rapid cuttage mating system described in embodiment 1 can improve the rooting rate of Chinese catalpa, the scope of application Extensively, can promote the use of a large area.
The upper only presently preferred embodiments of the present invention, is not intended to limit the invention, all spirit in the present invention Within principle, any modification, equivalent replacement, improvement and so on, should all be included in the protection scope of the present invention.

Claims (10)

1. a kind of Chinese catalpa rapid cuttage mating system, carries out in general purpose steel-plastic/profiled bar glass structure greenhouse of standardization, it is special Sign is,
Comprise the following steps:
(1) bar selection is planted
Select Chinese catalpa kind, clonal annual healthy and strong kind bar;
(2) cuttings is cultivated
To carry out in the production district in the cutting plot in greenhouse or greenhouse, selection is about 1~1.2m, butt diameter≤4cm, taper end diameter >= The healthy and strong kind bar disinfection of the no disease and pests harm of 0.5cm, spreads including horizontal positioned and vernalization trunk is used as in the germination accelerating pond of river sand, every kind of 1~2cm of spacing, the river sand of kind bar upper strata covering 2cm thickness, periodically sprays efficient germicide, during which maintains warm 25 DEG C of indoor temperature, Humidity is not less than 70%, and coppice shoot is obtained after cultivating 5~7 weeks;
(3) cuttings is handled
After the up to 7cm of coppice shoot 40% that step (2) obtains, retain whole blades, with sterilized cutter close to rudiment base portion band It expands tissue and is removed together from vernalization trunk, and each coppice shoot length is preferred with 7~9cm, and as a cuttings, long sprouts Bar is cut into the cuttings of two sections of 5~6cm long with the secateurs after disinfection, cuts off the subsidiary long bast group of all cuttings base portions Knit, retain cuttings base portion length and width and be preferred no more than 0.5cm, when obtaining 30~40 cuttings every time, carry out disinfection processing at once;
(4) cuttage
Carry out in cutting plot;Select peat:Perlite=2:1 is used as cutting medium, loaded in the hole tray after disinfection, and prepares life Root powder, the root-inducing powder are 1L talcum powder, mix No. ABT1 500 × 10-6No. mg/L+GGR7 80 × 10-6Powder made of mg/L Agent;
It is 2.5cm to make a call to a depth in the middle part of each cuttage position in hole tray, and the hole of a diameter of 1cm will be slotting obtained by step (3) Fringe base portion uniformly dips prepared root-inducing powder pulvis, and the hole being put into hole tray, fixes cuttings, and saturating matrix, spray are filled with clear water Fungicide is applied, it is 98% that the atomization humidity in greenhouse is kept during cuttage, and temperature is 22~25 DEG C;
(5) managed after cuttage
Atomising regime is carried out in 1~10d after cuttage, stops atomization after 10d, increases illumination, be aerated, during which periodically spray Fungicide, cuttage can periodically spray foliar fertilizer after 3 weeks, obtain cuttage seeding;
(6) acclimatization and transplants
Cutting plot hardening in greenhouse, also can be in hothouse production area hardening;After cuttage 5 weeks, whole sunshade nets, greenhouse are opened daily For temperature control at 28 DEG C, regular watering, fertilising, carry out hardening;During cuttage 7~8 weeks, portable cuttage seeding base portion, matrix and root system Fully combine, can be transplanted, 3d does not water before transplanting, goes out earing from hole tray, and strong sprout training is put into togerther together with matrix Educate in the planting pit in garden, insert topsoil, after cuttings is fixed, flood irrigates, after watered, the management such as apply fertilizer, Qiu Mo or Second year spring goes out garden.
A kind of 2. Chinese catalpa rapid cuttage mating system according to claim 1, it is characterised in that the annual strong non-hibernating eggs The incubation step of bar is as follows:Selection 1~2 year is raw to intend breeding kind, clone nursery stock, in more than butt stump at 6~9cm Rush is sprouted, and during rudiment up to 10~15cm, is selected and remain base portion germinating, is evenly distributed in base portion surrounding, the coppice shoot of robust growth 2~3, Remove unnecessary coppice shoot 2~3 times, late October wipes out 5~10cm of the tip of a branch, obtains Chinese catalpa kind, clonal annual healthy and strong kind bar.
A kind of 3. Chinese catalpa rapid cuttage mating system according to claim 1, it is characterised in that the river in the germination accelerating pond Husky water content 75%, river sand composition sand bed, 15 DEG C~20 DEG C of sand bed temperature.
4. a kind of Chinese catalpa rapid cuttage mating system according to claim 1 or 2, it is characterised in that the step (2) Stalwartness plants bar with 300 × 10-6Carbendazim (50% wettable powder) solution disinfection 35min of mg/L;The cuttings of the step (3) Disinfection way is to place vertically or slightly slant setting is 300 × 10-6Sterilized in carbendazim (50% wettable powder) solution of mg/L 15min。
A kind of 5. Chinese catalpa rapid cuttage mating system according to claim 3, it is characterised in that the river sand with 500 × 10-6The potassium permanganate irrigation and disinfection of mg/L;The hole tray is with 1000 × 10-6The potassium permanganate irrigation and disinfection of mg/L.
6. a kind of Chinese catalpa rapid cuttage mating system according to claim 2, it is characterised in that the mist pipe step is such as Under:After cuttage, preceding 5d, the atomization humidity in greenhouse is 98%, and temperature is 22~25 DEG C, and external sunshade is carried out with 70% sunshade net;6- During 10d, atomization humidity is progressively reduced to 75% by 98%, and temperature is 25~28 DEG C, by the outer screening for opening total aperture 10% The mode of screened postive increases illumination.
A kind of 7. Chinese catalpa rapid cuttage mating system according to claim 6, it is characterised in that after stopping atomization plant, Regarding weather conditions artificial watering 4~6 times daily daytime, greenhouse temperature should keep 25 DEG C~30 DEG C, and 10:00~14:00 opening side Window or skylight (open overall aperture 20%) ventilation;The external sunshade net of total aperture 40~50% is opened, increases illumination.
8. a kind of Chinese catalpa rapid cuttage mating system according to claim 7, it is characterised in that the cutter and the branch The sterilization method cut is dried after soaking 35min with 75% alcohol.
9. a kind of Chinese catalpa rapid cuttage mating system according to claim 8, it is characterised in that the step (4) sprays Fungicide be 500 × 10-6The carbendazim (50% wettable powder) of mg/L;It is every that the step (5), which periodically sprays fungicide, 7d is sprayed one time 500 × 10-6Mg/L first frosts MnZn (72% wettable powder) and 2500 × 10-6Mg/L hymexazo (green henries No. 1), it is described periodically to spray foliar fertilizer, per 5d one is sprayed with 0.3% urea and 0.5% potassium dihydrogen phosphate mixed solution Secondary, the step (5) further includes removal and goes mouldy cuttings with 800 × 10-6The potassium permanganate of mg/L pours the hole tray hole gone mouldy And its matrix.
A kind of 10. Chinese catalpa rapid cuttage mating system according to claim 9, it is characterised in that step (6) watering 5~7d of time interval, 0.2% urea and 0.2% potassium dihydrogen phosphate mixed solution are sprayed per 7d.
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CN109452079A (en) * 2018-12-29 2019-03-12 山东省林木种质资源中心 A kind of mating cottage breeding method of Chinese catalpa three-level
CN112470938A (en) * 2021-01-14 2021-03-12 河南省农业科学院园艺研究所 Micro-cutting seedling raising and rapid propagation method for catalpa bungei tissue culture seedlings
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