CN107614108B - 使用集成阵列对核酸杂交热力学的多路分析 - Google Patents

使用集成阵列对核酸杂交热力学的多路分析 Download PDF

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CN107614108B
CN107614108B CN201680029656.3A CN201680029656A CN107614108B CN 107614108 B CN107614108 B CN 107614108B CN 201680029656 A CN201680029656 A CN 201680029656A CN 107614108 B CN107614108 B CN 107614108B
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nucleic acid
target nucleic
acid molecule
sample
signal
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CN107614108A (zh
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奥尔延·哈斯比
卡沙玛·吉拉格
阿伦·马尼卡姆
卡韦赫·米拉尼尼亚
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

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  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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CN201680029656.3A 2015-03-23 2016-03-22 使用集成阵列对核酸杂交热力学的多路分析 Active CN107614108B (zh)

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US14/665,904 2015-03-23
US14/665,904 US9708647B2 (en) 2015-03-23 2015-03-23 Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
PCT/US2016/023634 WO2016154227A1 (en) 2015-03-23 2016-03-22 Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays

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CN107614108B true CN107614108B (zh) 2020-10-23

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US (2) US9708647B2 (enExample)
EP (2) EP3912723A1 (enExample)
JP (3) JP7123560B2 (enExample)
CN (1) CN107614108B (enExample)
GB (1) GB2555950A (enExample)
WO (1) WO2016154227A1 (enExample)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
WO2008014485A2 (en) 2006-07-28 2008-01-31 California Institute Of Technology Multiplex q-pcr arrays
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
JP6778194B2 (ja) 2014-12-31 2020-10-28 ビスビュー メディカル，インコーポレイテッド 分子診断試験のためのデバイス及び方法
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
US10373704B2 (en) * 2016-06-03 2019-08-06 International Business Machines Corporation Reduction of surface nucleotide hybridization by optimizing a biosensor sensing surface area
US10718758B2 (en) 2016-06-03 2020-07-21 International Business Machines Corporation Biosensor for optical detection of nucleotide sequence
US10712308B2 (en) 2016-06-03 2020-07-14 International Business Machines Corporation Biosensor for electrical detection of a nucleotide sequence
GB201708338D0 (en) 2017-05-24 2017-07-05 Univ Court Univ Of Glasgow Metabolite detection apparatus and method of detecting metabolites
CN110997934B (zh) * 2017-05-31 2025-02-28 生捷科技控股公司 具有电子检测系统的寡核苷酸探针阵列
JP7239568B2 (ja) 2017-11-09 2023-03-14 ビスビュー メディカル，インコーポレイテッド 携帯型分子診断デバイスおよび標的ウイルスの検出方法
JP2019093377A (ja) 2017-11-22 2019-06-20 株式会社エンプラス 流体チップ、流体デバイスおよびそれらの製造方法
MX2021002533A (es) 2018-09-03 2021-05-27 Visby Medical Inc Dispositivos y metodos para pruebas de susceptibilidad a antibioticos.
WO2020186252A1 (en) 2019-03-14 2020-09-17 Insilixa, Inc. Methods and systems for time-gated fluorescent-based detection
EP3937780A4 (en) 2019-03-14 2022-12-07 InSilixa, Inc. METHODS AND SYSTEMS FOR TIMED FLUORESCENCE-BASED DETECTION
AU2020265586A1 (en) 2019-04-28 2021-10-14 Visby Medical, Inc. Molecular diagnostic devices with digital detection capability and wireless connectivity
EP4085149A4 (en) 2020-01-03 2024-03-06 Visby Medical, Inc. Devices and methods for antibiotic susceptibility testing
WO2023028618A1 (en) * 2021-08-27 2023-03-02 The Board Of Trustees Of The Leland Stanford Junior University Systems and methods to determine nucleic acid conformations and uses thereof
GB2617798B (en) * 2021-09-23 2025-02-12 N6 Tec Inc Methods and systems for sample analysis
US20250297303A1 (en) 2024-03-21 2025-09-25 Cepheid Compositions and methods for selective extraction of oligonucleotides from complex matrices

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1250483A (zh) * 1997-01-15 2000-04-12 布拉克斯集团有限公司 与质量标记连接的杂文探针
CN1461350A (zh) * 2001-04-23 2003-12-10 三星电子株式会社 包含mosfet分子检测芯片和采用该芯片的分子检测装置以及使用该装置的分子检测方法
CN1993617A (zh) * 2004-08-05 2007-07-04 索尼株式会社 用于生产dna芯片的方法和系统、用于检测杂交的方法和系统和用于基质处理的设备和方法

Family Cites Families (212)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4027971A (en) 1973-01-08 1977-06-07 Philip Kolman Method of simultaneously counting blood cells
US4469863A (en) 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
US4711955A (en) 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
US4994373A (en) 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
GB8314523D0 (en) 1983-05-25 1983-06-29 Lowe C R Diagnostic device
US4539295A (en) 1983-06-30 1985-09-03 Beckman Instruments, Inc. Binary kinetic assay method and apparatus
US5235033A (en) 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US5656493A (en) 1985-03-28 1997-08-12 The Perkin-Elmer Corporation System for automated performance of the polymerase chain reaction
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5333675C1 (en) 1986-02-25 2001-05-01 Perkin Elmer Corp Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
CA1339653C (en) 1986-02-25 1998-02-03 Larry J. Johnson Appartus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5082830A (en) 1988-02-26 1992-01-21 Enzo Biochem, Inc. End labeled nucleotide probe
US6054270A (en) 1988-05-03 2000-04-25 Oxford Gene Technology Limited Analying polynucleotide sequences
US5216141A (en) 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US6551784B2 (en) 1989-06-07 2003-04-22 Affymetrix Inc Method of comparing nucleic acid sequences
US5871928A (en) 1989-06-07 1999-02-16 Fodor; Stephen P. A. Methods for nucleic acid analysis
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
EP0731175A3 (en) 1989-07-11 2004-05-26 Gen-Probe Incorporated Oligonucleotide probes for HIV nucleic acid
US5312905A (en) * 1990-07-27 1994-05-17 Fuji Photo Film Co., Ltd. 2-diazo-1,2-naphthoquinone compounds
US5386023A (en) 1990-07-27 1995-01-31 Isis Pharmaceuticals Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling
US5602240A (en) 1990-07-27 1997-02-11 Ciba Geigy Ag. Backbone modified oligonucleotide analogs
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
KR100236506B1 (ko) 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 폴리머라제 연쇄 반응 수행 장치
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5632957A (en) 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
US6048690A (en) 1991-11-07 2000-04-11 Nanogen, Inc. Methods for electronic fluorescent perturbation for analysis and electronic perturbation catalysis for synthesis
US5270184A (en) 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
WO1993009668A1 (en) 1991-11-22 1993-05-27 Affymax Technology N.V. Combinatorial strategies for polymer synthesis
US5644048A (en) 1992-01-10 1997-07-01 Isis Pharmaceuticals, Inc. Process for preparing phosphorothioate oligonucleotides
ES2082256T3 (es) 1992-03-23 1996-03-16 Hoffmann La Roche Metodo de deteccion del adn.
US5323115A (en) 1992-05-05 1994-06-21 Xerox Corporation Electrostatic voltmeter producing a low voltage output
US5674698A (en) 1992-09-14 1997-10-07 Sri International Up-converting reporters for biological and other assays using laser excitation techniques
US5837501A (en) 1993-07-09 1998-11-17 Akzo Nobel N.V. Nucleic acid quantitation by co-amplification of target with multiple internal controls
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5578832A (en) 1994-09-02 1996-11-26 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
US5637684A (en) 1994-02-23 1997-06-10 Isis Pharmaceuticals, Inc. Phosphoramidate and phosphorothioamidate oligomeric compounds
US5455705A (en) 1994-03-14 1995-10-03 Analog Devices, Inc. Transimpedance amplifier for optical receiver
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US6379897B1 (en) 2000-11-09 2002-04-30 Nanogen, Inc. Methods for gene expression monitoring on electronic microarrays
US5491063A (en) 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
US5599668A (en) 1994-09-22 1997-02-04 Abbott Laboratories Light scattering optical waveguide method for detecting specific binding events
US6600996B2 (en) 1994-10-21 2003-07-29 Affymetrix, Inc. Computer-aided techniques for analyzing biological sequences
US5795716A (en) 1994-10-21 1998-08-18 Chee; Mark S. Computer-aided visualization and analysis system for sequence evaluation
US5571673A (en) 1994-11-23 1996-11-05 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
EP0904542B1 (en) 1996-03-01 2005-06-29 Beckman Coulter, Inc. System for simultaneously conducting multiple ligand binding assays
US6114122A (en) 1996-03-26 2000-09-05 Affymetrix, Inc. Fluidics station with a mounting system and method of using
US5627054A (en) 1996-04-05 1997-05-06 The United States Of America As Represented By The Secretary Of The Army Competitor primer asymmetric polymerase chain reaction
US5925519A (en) 1996-06-03 1999-07-20 The Regents Of The University Of California Genetic alterations associated with prostate cancer
US6169981B1 (en) 1996-06-04 2001-01-02 Paul J. Werbos 3-brain architecture for an intelligent decision and control system
DE69739357D1 (de) 1996-06-04 2009-05-28 Univ Utah Res Found Überwachung der hybridisierung während pcr
US6984491B2 (en) 1996-07-29 2006-01-10 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
JP4663824B2 (ja) 1996-12-31 2011-04-06 ハイ スループット ジェノミクス インコーポレイテッド 多重化分子分析装置および方法
US5846726A (en) 1997-05-13 1998-12-08 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
AU9220498A (en) 1997-09-04 1999-03-22 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of usethereof
US20010046673A1 (en) 1999-03-16 2001-11-29 Ljl Biosystems, Inc. Methods and apparatus for detecting nucleic acid polymorphisms
EP1070244A4 (en) 1998-04-09 2002-04-17 California Inst Of Techn ELECTRONIC TECHNIQUES USED FOR THE DETECTION OF ANALYTES
US6330092B1 (en) 1998-05-08 2001-12-11 Agilent Technologies, Inc. Polarization based differential receiver for reduction of background in free-space optical links
US6319958B1 (en) 1998-06-22 2001-11-20 Wisconsin Alumni Research Foundation Method of sensitizing microbial cells to antimicrobial compound
DE69940623D1 (de) 1998-11-09 2009-04-30 Eiken Chemical Prozess zur Synthetisierung von Nukleinsäure
US6312906B1 (en) 1999-01-15 2001-11-06 Imperial College Innovations, Ltd. Immobilized nucleic acid hybridization reagent and method
EP1151139A2 (en) 1999-01-25 2001-11-07 UT-Battelle, LLC Multifunctional and multispectral biosensor devices and methods of use
US7107253B1 (en) 1999-04-05 2006-09-12 American Board Of Family Practice, Inc. Computer architecture and process of patient generation, evolution and simulation for computer based testing system using bayesian networks as a scripting language
US7423750B2 (en) 2001-11-29 2008-09-09 Applera Corporation Configurations, systems, and methods for optical scanning with at least one first relative angular motion and at least one second angular motion or at least one linear motion
US6277607B1 (en) 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
US6516276B1 (en) 1999-06-18 2003-02-04 Eos Biotechnology, Inc. Method and apparatus for analysis of data from biomolecular arrays
NZ528967A (en) 1999-06-22 2005-03-24 Invitrogen Corp Improved primers and methods for the amplification and discrimination of nucleic acids
AU783873B2 (en) 1999-09-13 2005-12-15 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
JP2003510054A (ja) 1999-09-22 2003-03-18 モトローラ・インコーポレイテッド 単一ヌクレオチド多形性の平行遺伝子型決定のための三次元ミクロアレイシステム
US6673536B1 (en) 1999-09-29 2004-01-06 Rosetta Inpharmatics Llc. Methods of ranking oligonucleotides for specificity using wash dissociation histories
US6784982B1 (en) 1999-11-04 2004-08-31 Regents Of The University Of Minnesota Direct mapping of DNA chips to detector arrays
DE10002566A1 (de) 2000-01-21 2001-08-02 Fraunhofer Ges Forschung Verfahren und Einrichtung zur Bestimmung des Schmelzpunktes und/oder der Bindungskonstante von Substanzen, wie z. B. DNA-Sequenzen, in einer Probe
US7033763B2 (en) 2000-02-23 2006-04-25 City Of Hope Pyrophosphorolysis activated polymerization (PAP)
US6579680B2 (en) 2000-02-28 2003-06-17 Corning Incorporated Method for label-free detection of hybridized DNA targets
US7998673B2 (en) 2000-03-29 2011-08-16 Lgc Limited Hybridisation beacon and method of rapid sequence detection and discrimination
US7348141B2 (en) 2000-03-29 2008-03-25 Lgc Limited Hybridization beacon and method of rapid sequence detection and discrimination
US7019129B1 (en) 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
US7354707B2 (en) 2000-06-27 2008-04-08 National Institute Of Advanced Industrial Science And Technology Nucleic acid probes, method for determining concentrations of nucleic acid by using the probes, and method for analyzing data obtained by the method
US6472887B1 (en) 2000-06-28 2002-10-29 Hewlett-Packard Company Capacitive sensor for sensing the amount of material in a container
DE10036457A1 (de) 2000-07-26 2002-02-14 Giesing Michael Verwendung eines bildgebenden photoelektrischen Flächensensors zur Auswertung von Biochips und Bildgebungsverfahren hierfür
AU2001288249A1 (en) 2000-08-14 2002-02-25 The Regents Of The University Of California Biosensors and methods for their use
US6724324B1 (en) 2000-08-21 2004-04-20 Delphi Technologies, Inc. Capacitive proximity sensor
US6469524B1 (en) 2000-08-25 2002-10-22 Delphi Technologies, Inc. System and method for interrogating a capacitive sensor
CN1348096A (zh) 2000-10-10 2002-05-08 栾国彦 一种均相特异性检测核酸的探针及应用方法
DE60125312T2 (de) 2000-10-26 2007-06-06 Agilent Technologies, Inc. (n.d. Ges. d. Staates Delaware), Santa Clara Mikroarray
US6818427B1 (en) 2000-10-27 2004-11-16 Millennium Pharmaceuticals, Inc. MEKK1 molecules and uses thereof
US8900811B2 (en) * 2000-11-16 2014-12-02 Caliper Life Sciences, Inc. Method and apparatus for generating thermal melting curves in a microfluidic device
US20050202470A1 (en) 2000-11-16 2005-09-15 Caliper Life Sciences, Inc. Binding assays using molecular melt curves
US6432695B1 (en) 2001-02-16 2002-08-13 Institute Of Microelectronics Miniaturized thermal cycler
US7361472B2 (en) 2001-02-23 2008-04-22 Invitrogen Corporation Methods for providing extended dynamic range in analyte assays
NZ523167A (en) 2001-03-09 2006-08-31 Nugen Technologies Inc Methods, compositions and kits for amplifying RNA sequences of interest which employ an RNA/DNA composite primer
US20020146745A1 (en) 2001-04-03 2002-10-10 Surromed, Inc. Methods and reagents for multiplexed analyte capture, surface array self-assembly, and analysis of complex biological samples
GB0111459D0 (en) 2001-05-10 2001-07-04 Isis Innovation Universal fluorescent sensors
AU2002339853A1 (en) 2001-05-24 2002-12-03 Genospectra, Inc. Pairs of nucleic acid probes with interactive signaling moieties and nucleic acid probes with enhanced hybridization efficiency and specificity
EP1397668A2 (en) 2001-06-06 2004-03-17 Digital Optical Imaging Corporation Light modulated microarray reader and methods relating thereto
US20020187477A1 (en) 2001-06-06 2002-12-12 Hong Xue Method for detecting single nucleotide polymorphisms (SNPs) and point mutations
US20030040000A1 (en) 2001-08-08 2003-02-27 Connolly Dennis M. Methods for attaching nucleic acid molecules to electrically conductive surfaces
US7173032B2 (en) 2001-09-21 2007-02-06 Reddy Us Therapeutics, Inc. Methods and compositions of novel triazine compounds
US6593091B2 (en) 2001-09-24 2003-07-15 Beckman Coulter, Inc. Oligonucleotide probes for detecting nucleic acids through changes in flourescence resonance energy transfer
US6744502B2 (en) 2001-09-28 2004-06-01 Pe Corporation (Ny) Shaped illumination geometry and intensity using a diffractive optical element
US20070010664A1 (en) 2001-10-01 2007-01-11 Thomas Elizabeth A Gene expression in the central nervous system regulated by neuroleptic agents
JP4377689B2 (ja) 2001-10-15 2009-12-02 バイオアレイ ソリューションズ リミテッド 同時尋問及び酵素仲介検出による多型遺伝子座の複合分析
US20030071843A1 (en) 2001-10-17 2003-04-17 Bruce Hoff System and method for specifying and applying microarray data preparation
EP1442142A4 (en) 2001-10-19 2006-11-15 Proligo Llc NUCLEIC ACID PROBES AND METHODS FOR DETECTING AND / OR QUANTIFYING NUCLEIC ACID ANALYTES
US7198897B2 (en) 2001-12-19 2007-04-03 Brandeis University Late-PCR
AU2003210571A1 (en) 2002-01-18 2003-09-02 University Of Utah Research Foundation Detection of single nucleotide polymorphisms using planar waveguides
US7257563B2 (en) 2002-01-30 2007-08-14 The Board Of Regents Of The University Of Texas Probabilistic boolean networks
WO2003089654A2 (en) 2002-04-22 2003-10-30 Wisconsin Alumni Research Foundation Single nucleotide polymorphism analysis using surface invasive cleavage reactions
US7785776B2 (en) 2002-05-13 2010-08-31 Idaho Technology, Inc. Genotyping by amplicon melting curve analysis
US6750963B2 (en) 2002-05-21 2004-06-15 Agilent Technologies, Inc. Imaging systems for signals on a surface
JP3904111B2 (ja) 2002-06-04 2007-04-11 ソニー株式会社 固体撮像装置及びその信号処理方法
US20040118688A1 (en) 2002-07-29 2004-06-24 Dumas David P. Transparent polymer support for electrophoresis and electrochromatography and related methods
US7267751B2 (en) 2002-08-20 2007-09-11 Nanogen, Inc. Programmable multiplexed active biologic array
CA2498764C (en) 2002-09-20 2015-11-10 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
DK1413584T3 (da) 2002-10-21 2005-07-04 Axxam S R L Fotoprotein med forbedret bioluminescens
US20040086864A1 (en) 2002-10-22 2004-05-06 The Chinese University Of Hong Kong Novel classification methods for pleural effusions
CN1164769C (zh) 2002-10-24 2004-09-01 高奔 四维参数检测基因芯片上核酸杂交对的方法及装置
US20040147045A1 (en) 2002-10-29 2004-07-29 Gentel Biosurfaces, Inc. Signal molecule arrays
US7390457B2 (en) 2002-10-31 2008-06-24 Agilent Technologies, Inc. Integrated microfluidic array device
DE60325541D1 (de) 2002-10-31 2009-02-12 Hoffmann La Roche Verfahren/Präparat zur Erkennung von Pankreaskrebs
US20040121337A1 (en) 2002-12-19 2004-06-24 Nomadics, Inc. Luminescent polymers and methods of use thereof
AU2003290429A1 (en) 2002-12-25 2004-07-22 Casio Computer Co., Ltd. Optical dna sensor, dna reading apparatus, identification method of dna and manufacturing method of optical dna sensor
DE10315074A1 (de) 2003-04-02 2004-10-14 Clondiag Chip Technologies Gmbh Vorrichtung zur Vervielfältigung und zum Nachweis von Nukleinsäuren
ES2384170T3 (es) 2003-04-04 2012-07-02 F. Hoffmann-La Roche Ag Sistema mejorado de PCR multicolor a tiempo real
WO2004097371A2 (en) 2003-04-25 2004-11-11 Board Of Regents, The University Of Texas System System and method for the detection of analytes
US7554007B2 (en) 2003-05-22 2009-06-30 Evogene Ltd. Methods of increasing abiotic stress tolerance and/or biomass in plants
US7125945B2 (en) 2003-09-19 2006-10-24 Varian, Inc. Functionalized polymer for oligonucleotide purification
US20050112585A1 (en) 2003-11-21 2005-05-26 Dominic Zichi Method for adjusting the quantification range of individual analytes in a multiplexed assay
US7330369B2 (en) 2004-04-06 2008-02-12 Bao Tran NANO-electronic memory array
US7995679B2 (en) 2004-04-27 2011-08-09 Broadcom Corporation Method and system for charge sensing with variable gain, offset compensation, and demodulation
CA2567114A1 (en) 2004-05-28 2005-12-15 Nanogen, Inc. Nanoscale electronic detection system and methods for their manufacture
US7479557B2 (en) 2004-06-10 2009-01-20 Agency For Science, Technology +Research DNA threading intercalators
US7141378B2 (en) 2004-07-02 2006-11-28 Blueshift Biotechnologies, Inc. Exploring fluorophore microenvironments
US8517329B2 (en) 2004-07-26 2013-08-27 3M Innovative Properties Company Easel stand mountable display board
CN1280422C (zh) 2004-08-26 2006-10-18 北京博奥生物芯片有限责任公司 一种不对称pcr扩增方法及其应用
US20070212681A1 (en) 2004-08-30 2007-09-13 Benjamin Shapiro Cell canaries for biochemical pathogen detection
US20080085839A1 (en) * 2004-09-01 2008-04-10 Holger Klapproth Method For The Analysis Of Point Mutations
US20120094298A1 (en) 2005-09-02 2012-04-19 Bioarray Solutions Limited Nucleic acid amplification with integrated multiplex detection
WO2006037527A1 (en) 2004-09-30 2006-04-13 Pamgene Bv Masked solid porous supports allowing fast and easy reagent exchange to accelerate electrode-based microarrays
US7585664B2 (en) * 2004-10-14 2009-09-08 The Hong Kong University Of Science And Technology Integrated circuit optical detector for biological detection
US20060088844A1 (en) 2004-10-22 2006-04-27 Honeywell International Inc. Real-time PCR microarray based on evanescent wave biosensor
EP1659183A1 (en) 2004-11-18 2006-05-24 Eppendorf Array Technologies Real-time quantification of multiple targets on a micro-array
ATE482288T1 (de) 2004-11-18 2010-10-15 Eppendorf Array Technologies Echtzeit-pcr von zielen auf einem mikroarray
JP2006162457A (ja) 2004-12-08 2006-06-22 Canon Inc 電位測定装置および画像形成装置
US7824890B2 (en) 2005-02-19 2010-11-02 Avacta Group Plc Isothermal amplification of nucleic acids
US7507565B2 (en) 2005-03-11 2009-03-24 Stanford University Bioluminescence resonance energy transfer (BRET) systems and methods of use thereof
US9223929B2 (en) 2005-03-14 2015-12-29 The California Institute Of Technology Method and apparatus for detection, identification and quantification of single-and multi-analytes in affinity-based sensor arrays
FR2892196B1 (fr) 2005-10-18 2008-06-20 Genewave Soc Par Actions Simpl Procede de fabrication d'un biocapteur a detection integree
US7463353B2 (en) 2006-05-31 2008-12-09 Uchicago Argonne, Llc Modular, micro-scale, optical array and biodetection system
WO2007143669A2 (en) 2006-06-05 2007-12-13 California Institute Of Technology Real time micro arrays
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
US8637436B2 (en) 2006-08-24 2014-01-28 California Institute Of Technology Integrated semiconductor bioarray
BRPI0712655A2 (pt) * 2006-06-08 2012-11-20 Koninkl Philips Electronics Nv dispositivo de sensor microeletrânico, mÉtodo para investigaÇço de pelo menos uma substÂncia alvo biolàgica, e, uso do dispositivo de sensor microeletrânico
US20090318307A1 (en) * 2006-07-27 2009-12-24 Koninklijke Philips Electronics N.V. Device for molecular diagnosis
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
WO2008014485A2 (en) 2006-07-28 2008-01-31 California Institute Of Technology Multiplex q-pcr arrays
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
WO2008082713A2 (en) 2006-08-24 2008-07-10 California Institute Of Technology Integrated semiconductor bioarray
EP2089542B1 (en) * 2006-11-29 2017-01-04 Canon U.S. Life Sciences, Inc. Device and method for digital multiplex pcr assays
US9114398B2 (en) 2006-11-29 2015-08-25 Canon U.S. Life Sciences, Inc. Device and method for digital multiplex PCR assays
KR101433208B1 (ko) * 2006-12-13 2014-08-22 루미넥스 코포레이션 실시간으로 ｐｃｒ의 다중 분석을 위한 시스템과 방법
AU2007334393A1 (en) 2006-12-14 2008-06-26 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
EP2374902B1 (en) 2007-01-26 2017-11-01 Illumina, Inc. Nucleic acid sequencing system and method
EP1956097A1 (en) 2007-02-06 2008-08-13 bioMerieux B.V. Method for discriminating single nucleotide polymorphisms (SNPs)
EP1995327A1 (en) 2007-05-21 2008-11-26 Humboldt Universität zu Berlin Probe for detecting a particular nucleic acid sequence
WO2009021054A2 (en) 2007-08-06 2009-02-12 Orion Genomics Llc Novel single nucleotide polymorphisms and combinations of novel and known polymorphisms for determining the allele-specific expression of the igf2 gene
DE102007044664B4 (de) * 2007-09-18 2012-01-05 Friz Biochem Gesellschaft Für Bioanalytik Mbh Verdrängungsassay zur Detektion von Nukleinsäureoligomer-Hybridisierungsereignissen
WO2009061783A2 (en) 2007-11-05 2009-05-14 University Of Rochester Dna microarray having hairpin probes tethered to nanostructured metal surface
WO2009082706A1 (en) 2007-12-21 2009-07-02 The Trustees Of Columbia University In The City Of New York Active cmos sensor array for electrochemical biomolecular detection
US8741570B2 (en) * 2008-02-06 2014-06-03 Ludwig-Maximilians-Universitat Munchen Thermo-optical characterisation of nucleic acid molecules
WO2009155128A1 (en) 2008-06-18 2009-12-23 Ge Healthcare Bio-Sciences Corp. Method for separation of double-stranded and single-stranded nucleic acids from the same sample
EP2138587A1 (en) 2008-06-23 2009-12-30 Koninklijke Philips Electronics N.V. Amplification of nucleic acids using temperature zones
US9393566B2 (en) 2008-06-23 2016-07-19 Canon U.S. Life Sciences, Inc. System and method for temperature referencing for melt curve data collection
CN102076870A (zh) 2008-06-25 2011-05-25 实时基因组有限公司 对微阵列上的核酸进行解链曲线分析的方法和装置
US8058005B2 (en) * 2008-10-23 2011-11-15 Honeywell International Inc. Method for single nucleotide polymorphism and mutation detection using real time polymerase chain reaction microarray
US20100122904A1 (en) 2008-11-17 2010-05-20 Board Of Regents, The University Of Texas System Incorporating cmos integrated circuits in the design of affinity-based biosensor systems
RU2011125332A (ru) 2008-11-21 2012-12-27 Конинклейке Филипс Электроникс Н.В. Определение множественной пцр в реальном времени на твердых поверхностях с использованием красителей со специфичностью к двухцепочечным нуклеиновым кислотам
EP2391883B1 (en) * 2009-01-30 2018-03-07 Micronics, Inc. Portable high gain fluorescence detection system
ES2574055T3 (es) 2009-11-25 2016-06-14 Gen9, Inc. Métodos y aparatos para la reducción de errores en el ADN basada en un chip
US20110312546A1 (en) 2010-06-17 2011-12-22 Geneasys Pty Ltd Loc device for pathogen detection and genetic analysis with chemical lysis, incubation and tandem nucleic acid amplification
US8962252B2 (en) * 2010-08-31 2015-02-24 Canon U.S. Life Sciences, Inc. Optical system for high resolution thermal melt detection
KR20120042100A (ko) 2010-10-22 2012-05-03 주식회사 씨젠 이중표지 고정화 프로브를 이용한 고상에서의 타겟 핵산서열 검출
CA2965074A1 (en) 2010-12-27 2012-07-05 Abbott Molecular Inc. Quantitating high titer samples by digital pcr
US20120295805A1 (en) 2011-05-18 2012-11-22 Polytechnic Institute Of New York University Solid phase methods for thermodynamic and kinetic quantification of interactions between nucleic acids and small molecules
CN102433376B (zh) 2011-10-19 2013-07-31 上海千友生物科技有限公司 基于荧光淬灭的基因变异检测方法及探针
US9433943B2 (en) * 2011-11-15 2016-09-06 The Board Of Trustees Of The University Of Illinois Thermal control of droplets by nanoscale field effect transistors
EP2786146A4 (en) 2011-11-29 2015-08-26 Agrigenetics Inc SINGLE-NUCLEOTIDE POLYMORPHISM ASSAY HIGH-PERFORMANCE
CA2858119A1 (en) 2011-12-09 2013-06-13 Sietzema SIETZE Method for detection of bacteria in milk
JP6401146B2 (ja) 2012-04-05 2018-10-03 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ フローサイトメトリーのための試料調製
WO2013158280A1 (en) * 2012-04-20 2013-10-24 The Trustees Of Columbia University In The City Of New York Systems and methods for single-molecule nucleic-acid assay platforms
US9341589B2 (en) 2012-06-20 2016-05-17 Board Of Regents, The University Of Texas System Active-electrode integrated biosensor array and methods for use thereof
US8969781B2 (en) 2012-06-28 2015-03-03 Board Of Regents, The University Of Texas System Integrated optical biosensor array including charge injection circuit and quantizer circuit
WO2014089368A1 (en) 2012-12-05 2014-06-12 Bio-Rad Laboratories, Inc Methods for polymerase chain reaction copy number variation assays
US9718056B2 (en) 2013-03-15 2017-08-01 Syracuse University Microfluidics polymerase chain reaction and high resolution melt detection
US9983163B2 (en) 2013-04-30 2018-05-29 Board Of Regents, The University Of Texas System Integrated electro-analytical biosensor array
TWI527905B (zh) * 2013-11-06 2016-04-01 國立台灣大學 透過利用基因檢測技術與微珠微流道結合進行單核苷酸多態性檢測之方法
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
EP3347714B1 (en) 2015-09-10 2021-03-10 InSilixa Inc. Methods for multiplex quantitative nucleic acid amplification
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1250483A (zh) * 1997-01-15 2000-04-12 布拉克斯集团有限公司 与质量标记连接的杂文探针
CN1461350A (zh) * 2001-04-23 2003-12-10 三星电子株式会社 包含mosfet分子检测芯片和采用该芯片的分子检测装置以及使用该装置的分子检测方法
CN1993617A (zh) * 2004-08-05 2007-07-04 索尼株式会社 用于生产dna芯片的方法和系统、用于检测杂交的方法和系统和用于基质处理的设备和方法

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