ATE482288T1 - Echtzeit-pcr von zielen auf einem mikroarray - Google Patents
Echtzeit-pcr von zielen auf einem mikroarrayInfo
- Publication number
- ATE482288T1 ATE482288T1 AT05813624T AT05813624T ATE482288T1 AT E482288 T1 ATE482288 T1 AT E482288T1 AT 05813624 T AT05813624 T AT 05813624T AT 05813624 T AT05813624 T AT 05813624T AT E482288 T1 ATE482288 T1 AT E482288T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleotide molecule
- target nucleotide
- molecule
- solution
- labelled target
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0636—Integrated biosensor, microarrays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0822—Slides
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/991,087 US8288128B2 (en) | 2004-11-18 | 2004-11-18 | Real-time quantification of multiple targets on a micro-array |
EP04027435A EP1659183A1 (de) | 2004-11-18 | 2004-11-18 | Quantifizierung einer Vielzahl von Zielmolekülen auf einem Mikroarray in real-time |
PCT/EP2005/012383 WO2006053770A1 (en) | 2004-11-18 | 2005-11-18 | Real-time pcr of targets on a micro-array |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE482288T1 true ATE482288T1 (de) | 2010-10-15 |
Family
ID=35539286
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT05813624T ATE482288T1 (de) | 2004-11-18 | 2005-11-18 | Echtzeit-pcr von zielen auf einem mikroarray |
AT10172182T ATE549420T1 (de) | 2004-11-18 | 2005-11-18 | Echtzeit-pcr von nukleinsäuren auf einem mikroarray |
AT06114103T ATE505560T1 (de) | 2004-11-18 | 2006-05-17 | Reaktionsgefäss zur real-time pcr umfassend oligonukleotidproben wobei die amplifikationsprodukte durch hybridisierung und ohne öffnen des reaktionsgefässes gemessen werden |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT10172182T ATE549420T1 (de) | 2004-11-18 | 2005-11-18 | Echtzeit-pcr von nukleinsäuren auf einem mikroarray |
AT06114103T ATE505560T1 (de) | 2004-11-18 | 2006-05-17 | Reaktionsgefäss zur real-time pcr umfassend oligonukleotidproben wobei die amplifikationsprodukte durch hybridisierung und ohne öffnen des reaktionsgefässes gemessen werden |
Country Status (6)
Country | Link |
---|---|
US (1) | US20090191618A1 (de) |
EP (3) | EP2302072B1 (de) |
JP (1) | JP5026978B2 (de) |
AT (3) | ATE482288T1 (de) |
DE (1) | DE602005023705D1 (de) |
WO (2) | WO2006053770A1 (de) |
Families Citing this family (38)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7875442B2 (en) | 2000-03-24 | 2011-01-25 | Eppendorf Array Technologies | Identification and quantification of a plurality of biological (micro)organisms or their components |
US7829313B2 (en) | 2000-03-24 | 2010-11-09 | Eppendorf Array Technologies | Identification and quantification of a plurality of biological (micro)organisms or their components |
CA2348042A1 (en) | 2001-06-04 | 2002-12-04 | Ann Huletsky | Sequences for detection and identification of methicillin-resistant staphylococcus aureus |
US11834720B2 (en) | 2005-10-11 | 2023-12-05 | Geneohm Sciences, Inc. | Sequences for detection and identification of methicillin-resistant Staphylococcus aureus (MRSA) of MREJ types xi to xx |
EP2041301B1 (de) * | 2006-05-17 | 2014-12-31 | Eppendorf Array Technologies SA | Identifizierung und quantifizierung mehrerer biologischer (mikro-)organismen oder deren komponenten |
US11001881B2 (en) | 2006-08-24 | 2021-05-11 | California Institute Of Technology | Methods for detecting analytes |
EP2029775B1 (de) * | 2006-06-05 | 2014-10-15 | California Institute Of Technology | Echtzeit-mikroarrays |
US8637436B2 (en) | 2006-08-24 | 2014-01-28 | California Institute Of Technology | Integrated semiconductor bioarray |
US11525156B2 (en) | 2006-07-28 | 2022-12-13 | California Institute Of Technology | Multiplex Q-PCR arrays |
US8048626B2 (en) | 2006-07-28 | 2011-11-01 | California Institute Of Technology | Multiplex Q-PCR arrays |
US11560588B2 (en) | 2006-08-24 | 2023-01-24 | California Institute Of Technology | Multiplex Q-PCR arrays |
JP2008102117A (ja) * | 2006-09-21 | 2008-05-01 | Fujifilm Corp | 表面プラズモン増強蛍光センサおよび蛍光検出方法 |
EP1935496A1 (de) * | 2006-12-22 | 2008-06-25 | Eppendorf Array Technologies SA | Vorrichtung und/oder Verfahren zur Detektion erweiterter Nukleotidsequenzen auf Mikro-Arrays |
EP1946841A1 (de) * | 2006-12-22 | 2008-07-23 | Eppendorf Array Technologies SA | Vorrichtung und/oder Verfahren zum Nachweis von amplifizierten Nukleotidsequenzen auf Mikroarrays |
JP2009060859A (ja) * | 2007-09-07 | 2009-03-26 | Sumitomo Bakelite Co Ltd | 遺伝子の検出方法 |
CN103543094B (zh) * | 2007-12-19 | 2017-06-09 | 神谷来克斯公司 | 单分子检测用扫描分析器和使用方法 |
WO2009100197A2 (en) | 2008-02-05 | 2009-08-13 | Pocared Diagnostics Ltd. | System for conducting the identification of bacteria in biological samples |
EP2107125A1 (de) | 2008-03-31 | 2009-10-07 | Eppendorf Array Technologies SA (EAT) | Echtzeit-PCR von Targets auf einem Mikroarray |
US20100069253A1 (en) * | 2008-09-12 | 2010-03-18 | Gindilis Andrei L | Impedance Spectroscopy Measurement of DNA |
WO2010076189A1 (en) * | 2008-12-30 | 2010-07-08 | Stmicroelectronics S.R.L. | Method, microreactor and apparatus for carrying out real-time nucleic acid amplification |
WO2010084381A1 (en) * | 2009-01-21 | 2010-07-29 | Eppendorf Ag | Detection and/or quantification method of target molecules on a solid support |
EP2221605A1 (de) * | 2009-02-12 | 2010-08-25 | Koninklijke Philips Electronics N.V. | Drahtgittersensor |
US10288632B2 (en) | 2009-09-21 | 2019-05-14 | Pocared Diagnostics Ltd. | System for conducting the identification of bacteria in biological samples |
EP2369325A1 (de) | 2010-03-12 | 2011-09-28 | Eppendorf Ag | Arrayanalyse für Online-Detektion |
EP2441520A1 (de) | 2010-10-12 | 2012-04-18 | Eppendorf AG | Echtzeitverstärkungs- und Mikroarray-basierte Erkennung von Nukleinsäuretargets in einem Fluss-Chip-Assay |
JP5931910B2 (ja) | 2010-12-17 | 2016-06-08 | ビージェイエス アイピー リミテッド | 高速pcr加熱のための方法及びシステム |
WO2013080432A1 (ja) * | 2011-11-28 | 2013-06-06 | ソニー株式会社 | ケミカルセンサ、ケミカルセンサモジュール、化学物質検出装置及び化学物質検出方法 |
CN104302402B (zh) * | 2012-03-16 | 2017-07-04 | 生命技术公司 | 用于评估生物样品的系统和方法 |
EP2855019A1 (de) | 2012-05-24 | 2015-04-08 | BJS IP Limited | Klemme für schnelles pcr-erwärmen |
US9708647B2 (en) | 2015-03-23 | 2017-07-18 | Insilixa, Inc. | Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays |
US9499861B1 (en) | 2015-09-10 | 2016-11-22 | Insilixa, Inc. | Methods and systems for multiplex quantitative nucleic acid amplification |
KR20170099738A (ko) | 2016-02-23 | 2017-09-01 | 노을 주식회사 | 접촉식 염색 패치 및 그 제조 방법 |
KR102478636B1 (ko) * | 2016-02-23 | 2022-12-19 | 노을 주식회사 | 중합 효소 연쇄 반응 패치, 이를 이용하는 진단 방법 및 장치 |
KR102045069B1 (ko) * | 2016-02-23 | 2019-11-15 | 노을 주식회사 | 패치를 이용하는 ctc 진단 방법 및 장치 |
US10371610B2 (en) | 2016-02-23 | 2019-08-06 | Noul Co., Ltd. | Contact-type patch, staining method using the same, and manufacturing method thereof |
WO2017155858A1 (en) | 2016-03-07 | 2017-09-14 | Insilixa, Inc. | Nucleic acid sequence identification using solid-phase cyclic single base extension |
CN113924041A (zh) | 2019-03-14 | 2022-01-11 | 因斯利克萨公司 | 基于时间门控的荧光检测的方法和系统 |
CN116515617B (zh) * | 2023-07-03 | 2023-10-20 | 杭州杰毅生物技术有限公司 | 一种离心式pcr-荧光多靶标检测芯片 |
Family Cites Families (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NL154598B (nl) | 1970-11-10 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking. |
US3817837A (en) | 1971-05-14 | 1974-06-18 | Syva Corp | Enzyme amplification assay |
US3939350A (en) | 1974-04-29 | 1976-02-17 | Board Of Trustees Of The Leland Stanford Junior University | Fluorescent immunoassay employing total reflection for activation |
US3996345A (en) | 1974-08-12 | 1976-12-07 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
US4277437A (en) | 1978-04-05 | 1981-07-07 | Syva Company | Kit for carrying out chemically induced fluorescence immunoassay |
US4275149A (en) | 1978-11-24 | 1981-06-23 | Syva Company | Macromolecular environment control in specific receptor assays |
US4366241A (en) | 1980-08-07 | 1982-12-28 | Syva Company | Concentrating zone method in heterogeneous immunoassays |
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
US5994056A (en) | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
ATE198511T1 (de) | 1994-04-29 | 2001-01-15 | Perkin Elmer Corp | Verfahren und vorrichtung zur echtzeiterfassung der produkte von nukleinsäureamplifikation |
ATE196322T1 (de) | 1995-05-05 | 2000-09-15 | Perkin Elmer Corp | Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay |
JP2002515738A (ja) | 1996-01-23 | 2002-05-28 | アフィメトリックス,インコーポレイティド | 核酸分析法 |
US5716784A (en) | 1996-02-05 | 1998-02-10 | The Perkin-Elmer Corporation | Fluorescence detection assay for homogeneous PCR hybridization systems |
EP0951569A2 (de) * | 1996-12-23 | 1999-10-27 | University Of Chicago | Kundengerichtete oligonukleotidchips als vielfaeltige biosensoren |
AU1926899A (en) | 1997-12-19 | 1999-07-12 | Affymetrix, Inc. | Exploiting genomics in the search for new drugs |
US5952202A (en) | 1998-03-26 | 1999-09-14 | The Perkin Elmer Corporation | Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification |
US20020177135A1 (en) * | 1999-07-27 | 2002-11-28 | Doung Hau H. | Devices and methods for biochip multiplexing |
DE19949735A1 (de) | 1999-10-15 | 2001-05-10 | Bruker Daltonik Gmbh | Prozessieren von Proben in Lösungen mit definiert kleiner Wandkontaktfläche |
US6316200B1 (en) * | 2000-06-08 | 2001-11-13 | Becton, Dickinson And Company | Probes and methods for detection of nucleic acids |
AU6834700A (en) * | 1999-08-13 | 2001-03-13 | Zeptosens Ag | Device and method for determining multiple analytes |
US7205104B2 (en) | 2000-03-24 | 2007-04-17 | Eppendorf Array Technologies Sa (Eat) | Identification of biological (micro) organisms by detection of their homologous nucleotide sequences on arrays |
JP3963422B2 (ja) * | 2000-08-03 | 2007-08-22 | 日鉄環境エンジニアリング株式会社 | 核酸の測定方法 |
EP1184349A1 (de) | 2000-09-01 | 2002-03-06 | A.S.B.L. Facultes Universitaires Notre-Dame De La Paix | Verfahren zur Erhaltung einer Oberflächenaktivierung eines festen Trägers zur Bildung von Biochips Mikroanordnungen |
US6754414B2 (en) * | 2001-09-27 | 2004-06-22 | Bio-Rad Laboratories, Inc. | Imaging of microarrays using fiber optic exciter |
MXPA04006861A (es) * | 2002-01-18 | 2005-06-20 | Univ Utah Res Found | Deteccion de polimorfismos de nucleotidos individuales utilizando guias de onda planas. |
AU2003233451A1 (en) * | 2002-03-26 | 2003-10-13 | Massachusetts Institute Of Technology | Targets, methods, and reagents for diagnosis and treatment of schizophrenia |
DE10315074A1 (de) * | 2003-04-02 | 2004-10-14 | Clondiag Chip Technologies Gmbh | Vorrichtung zur Vervielfältigung und zum Nachweis von Nukleinsäuren |
JP4074829B2 (ja) * | 2003-04-16 | 2008-04-16 | 大日本印刷株式会社 | バイオマイクロアレイ用基板およびバイオマイクロアレイ |
US7622296B2 (en) * | 2004-05-28 | 2009-11-24 | Wafergen, Inc. | Apparatus and method for multiplex analysis |
US20060088844A1 (en) * | 2004-10-22 | 2006-04-27 | Honeywell International Inc. | Real-time PCR microarray based on evanescent wave biosensor |
-
2005
- 2005-11-18 AT AT05813624T patent/ATE482288T1/de not_active IP Right Cessation
- 2005-11-18 DE DE602005023705T patent/DE602005023705D1/de active Active
- 2005-11-18 EP EP10172182A patent/EP2302072B1/de not_active Revoked
- 2005-11-18 AT AT10172182T patent/ATE549420T1/de active
- 2005-11-18 WO PCT/EP2005/012383 patent/WO2006053770A1/en active Application Filing
- 2005-11-18 EP EP05813624A patent/EP1704258B1/de not_active Not-in-force
- 2005-11-18 EP EP05806579A patent/EP1812594B1/de not_active Not-in-force
- 2005-11-18 JP JP2007541801A patent/JP5026978B2/ja not_active Expired - Fee Related
- 2005-11-18 WO PCT/EP2005/012382 patent/WO2006053769A1/en active Application Filing
-
2006
- 2006-05-17 AT AT06114103T patent/ATE505560T1/de not_active IP Right Cessation
-
2007
- 2007-05-14 US US12/301,186 patent/US20090191618A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
DE602005023705D1 (de) | 2010-11-04 |
EP1812594A1 (de) | 2007-08-01 |
ATE505560T1 (de) | 2011-04-15 |
WO2006053769A1 (en) | 2006-05-26 |
JP5026978B2 (ja) | 2012-09-19 |
JP2009530589A (ja) | 2009-08-27 |
ATE549420T1 (de) | 2012-03-15 |
EP2302072A1 (de) | 2011-03-30 |
EP1704258A1 (de) | 2006-09-27 |
WO2006053770A8 (en) | 2006-08-24 |
WO2006053770A1 (en) | 2006-05-26 |
US20090191618A1 (en) | 2009-07-30 |
EP2302072B1 (de) | 2012-03-14 |
EP1812594B1 (de) | 2013-01-09 |
EP1704258B1 (de) | 2010-09-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ATE482288T1 (de) | Echtzeit-pcr von zielen auf einem mikroarray | |
US8247196B2 (en) | Real-time PCR of targets on a micro-array | |
WO2006135437A3 (en) | Real-time pcr microarray based on an evanescent wave biosensor | |
JP2006523095A5 (de) | ||
WO2004087951A3 (de) | Vorrichtung zur vervielfältigung und zum nachweis von nukleinsäuren | |
RU2008148143A (ru) | Микроэлектронное сенсорное устройство для детектирования днк | |
Peter et al. | Optical DNA-sensor chip for real-time detection of hybridization events | |
WO2005108609A1 (en) | Method for identification and analysis of certain molecules using the dual function of single strand nucleic acid | |
ATE398186T1 (de) | Integriertes verfahren und system zur amplifizierung und zum nachweis von nukleinsäuren | |
RU2582250C2 (ru) | Способ тестирования и контроль качества нуклеиновых кислот на подложке | |
US8288128B2 (en) | Real-time quantification of multiple targets on a micro-array | |
WO2011009893A1 (en) | A membrane based microarray device for high-throughput multianalyte detection | |
US20080199861A1 (en) | Real-time microarray apparatus and methods related thereto | |
Patel | A review on plant disease diagnosis through biosensor | |
ATE541050T1 (de) | Analyse von biologischen zielmolekülen unter verwendung eines einen fluoreszenzmarker tragenden biochips | |
JP4538746B2 (ja) | バイオチップおよび分析装置 | |
KR101017199B1 (ko) | 핵산 마이크로어레이를 이용한 핵산 검출 방법 | |
EP3287768B1 (de) | Erkennung von unterschiedlichen molekülen mit glasfasersensoren | |
JP3705499B2 (ja) | ターゲット核酸断片の分析方法及びターゲット核酸断片の分析キット | |
EP2119797A4 (de) | Verfahren zum nachweis von amplifikation oder deletion in einem genomischen dna-fragment | |
US20080164424A1 (en) | Reducing Background Fluorescence in Arrays | |
JP4079808B2 (ja) | 核酸増幅およびハイブリダイゼーション検出が可能なプローブ固相化反応アレイ | |
WO2024028886A1 (en) | A miniature quantitative polymerase chain reaction apparatus | |
JP2006029953A (ja) | 生体関連物質検出用固相化担体及びプローブの固相化方法、並びに生体関連物質解析方法 | |
Vasantgadkar | A novel method for detecting DNA |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |