ATE482288T1 - Echtzeit-pcr von zielen auf einem mikroarray - Google Patents

Echtzeit-pcr von zielen auf einem mikroarray

Info

Publication number
ATE482288T1
ATE482288T1 AT05813624T AT05813624T ATE482288T1 AT E482288 T1 ATE482288 T1 AT E482288T1 AT 05813624 T AT05813624 T AT 05813624T AT 05813624 T AT05813624 T AT 05813624T AT E482288 T1 ATE482288 T1 AT E482288T1
Authority
AT
Austria
Prior art keywords
nucleotide molecule
target nucleotide
molecule
solution
labelled target
Prior art date
Application number
AT05813624T
Other languages
English (en)
Inventor
Jose Remacle
Isabelle Alexandre
Sylvain Margaine
Dieter Husar
Original Assignee
Eppendorf Array Technologies
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=35539286&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE482288(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US10/991,087 external-priority patent/US8288128B2/en
Priority claimed from EP04027435A external-priority patent/EP1659183A1/de
Application filed by Eppendorf Array Technologies filed Critical Eppendorf Array Technologies
Application granted granted Critical
Publication of ATE482288T1 publication Critical patent/ATE482288T1/de

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • G01N21/6452Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/52Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/06Auxiliary integrated devices, integrated components
    • B01L2300/0627Sensor or part of a sensor is integrated
    • B01L2300/0636Integrated biosensor, microarrays
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0809Geometry, shape and general structure rectangular shaped
    • B01L2300/0822Slides
AT05813624T 2004-11-18 2005-11-18 Echtzeit-pcr von zielen auf einem mikroarray ATE482288T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10/991,087 US8288128B2 (en) 2004-11-18 2004-11-18 Real-time quantification of multiple targets on a micro-array
EP04027435A EP1659183A1 (de) 2004-11-18 2004-11-18 Quantifizierung einer Vielzahl von Zielmolekülen auf einem Mikroarray in real-time
PCT/EP2005/012383 WO2006053770A1 (en) 2004-11-18 2005-11-18 Real-time pcr of targets on a micro-array

Publications (1)

Publication Number Publication Date
ATE482288T1 true ATE482288T1 (de) 2010-10-15

Family

ID=35539286

Family Applications (3)

Application Number Title Priority Date Filing Date
AT05813624T ATE482288T1 (de) 2004-11-18 2005-11-18 Echtzeit-pcr von zielen auf einem mikroarray
AT10172182T ATE549420T1 (de) 2004-11-18 2005-11-18 Echtzeit-pcr von nukleinsäuren auf einem mikroarray
AT06114103T ATE505560T1 (de) 2004-11-18 2006-05-17 Reaktionsgefäss zur real-time pcr umfassend oligonukleotidproben wobei die amplifikationsprodukte durch hybridisierung und ohne öffnen des reaktionsgefässes gemessen werden

Family Applications After (2)

Application Number Title Priority Date Filing Date
AT10172182T ATE549420T1 (de) 2004-11-18 2005-11-18 Echtzeit-pcr von nukleinsäuren auf einem mikroarray
AT06114103T ATE505560T1 (de) 2004-11-18 2006-05-17 Reaktionsgefäss zur real-time pcr umfassend oligonukleotidproben wobei die amplifikationsprodukte durch hybridisierung und ohne öffnen des reaktionsgefässes gemessen werden

Country Status (6)

Country Link
US (1) US20090191618A1 (de)
EP (3) EP2302072B1 (de)
JP (1) JP5026978B2 (de)
AT (3) ATE482288T1 (de)
DE (1) DE602005023705D1 (de)
WO (2) WO2006053770A1 (de)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7875442B2 (en) 2000-03-24 2011-01-25 Eppendorf Array Technologies Identification and quantification of a plurality of biological (micro)organisms or their components
US7829313B2 (en) 2000-03-24 2010-11-09 Eppendorf Array Technologies Identification and quantification of a plurality of biological (micro)organisms or their components
CA2348042A1 (en) 2001-06-04 2002-12-04 Ann Huletsky Sequences for detection and identification of methicillin-resistant staphylococcus aureus
US11834720B2 (en) 2005-10-11 2023-12-05 Geneohm Sciences, Inc. Sequences for detection and identification of methicillin-resistant Staphylococcus aureus (MRSA) of MREJ types xi to xx
EP2041301B1 (de) * 2006-05-17 2014-12-31 Eppendorf Array Technologies SA Identifizierung und quantifizierung mehrerer biologischer (mikro-)organismen oder deren komponenten
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
EP2029775B1 (de) * 2006-06-05 2014-10-15 California Institute Of Technology Echtzeit-mikroarrays
US8637436B2 (en) 2006-08-24 2014-01-28 California Institute Of Technology Integrated semiconductor bioarray
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
US8048626B2 (en) 2006-07-28 2011-11-01 California Institute Of Technology Multiplex Q-PCR arrays
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
JP2008102117A (ja) * 2006-09-21 2008-05-01 Fujifilm Corp 表面プラズモン増強蛍光センサおよび蛍光検出方法
EP1935496A1 (de) * 2006-12-22 2008-06-25 Eppendorf Array Technologies SA Vorrichtung und/oder Verfahren zur Detektion erweiterter Nukleotidsequenzen auf Mikro-Arrays
EP1946841A1 (de) * 2006-12-22 2008-07-23 Eppendorf Array Technologies SA Vorrichtung und/oder Verfahren zum Nachweis von amplifizierten Nukleotidsequenzen auf Mikroarrays
JP2009060859A (ja) * 2007-09-07 2009-03-26 Sumitomo Bakelite Co Ltd 遺伝子の検出方法
CN103543094B (zh) * 2007-12-19 2017-06-09 神谷来克斯公司 单分子检测用扫描分析器和使用方法
WO2009100197A2 (en) 2008-02-05 2009-08-13 Pocared Diagnostics Ltd. System for conducting the identification of bacteria in biological samples
EP2107125A1 (de) 2008-03-31 2009-10-07 Eppendorf Array Technologies SA (EAT) Echtzeit-PCR von Targets auf einem Mikroarray
US20100069253A1 (en) * 2008-09-12 2010-03-18 Gindilis Andrei L Impedance Spectroscopy Measurement of DNA
WO2010076189A1 (en) * 2008-12-30 2010-07-08 Stmicroelectronics S.R.L. Method, microreactor and apparatus for carrying out real-time nucleic acid amplification
WO2010084381A1 (en) * 2009-01-21 2010-07-29 Eppendorf Ag Detection and/or quantification method of target molecules on a solid support
EP2221605A1 (de) * 2009-02-12 2010-08-25 Koninklijke Philips Electronics N.V. Drahtgittersensor
US10288632B2 (en) 2009-09-21 2019-05-14 Pocared Diagnostics Ltd. System for conducting the identification of bacteria in biological samples
EP2369325A1 (de) 2010-03-12 2011-09-28 Eppendorf Ag Arrayanalyse für Online-Detektion
EP2441520A1 (de) 2010-10-12 2012-04-18 Eppendorf AG Echtzeitverstärkungs- und Mikroarray-basierte Erkennung von Nukleinsäuretargets in einem Fluss-Chip-Assay
JP5931910B2 (ja) 2010-12-17 2016-06-08 ビージェイエス アイピー リミテッド 高速pcr加熱のための方法及びシステム
WO2013080432A1 (ja) * 2011-11-28 2013-06-06 ソニー株式会社 ケミカルセンサ、ケミカルセンサモジュール、化学物質検出装置及び化学物質検出方法
CN104302402B (zh) * 2012-03-16 2017-07-04 生命技术公司 用于评估生物样品的系统和方法
EP2855019A1 (de) 2012-05-24 2015-04-08 BJS IP Limited Klemme für schnelles pcr-erwärmen
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
KR20170099738A (ko) 2016-02-23 2017-09-01 노을 주식회사 접촉식 염색 패치 및 그 제조 방법
KR102478636B1 (ko) * 2016-02-23 2022-12-19 노을 주식회사 중합 효소 연쇄 반응 패치, 이를 이용하는 진단 방법 및 장치
KR102045069B1 (ko) * 2016-02-23 2019-11-15 노을 주식회사 패치를 이용하는 ctc 진단 방법 및 장치
US10371610B2 (en) 2016-02-23 2019-08-06 Noul Co., Ltd. Contact-type patch, staining method using the same, and manufacturing method thereof
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
CN113924041A (zh) 2019-03-14 2022-01-11 因斯利克萨公司 基于时间门控的荧光检测的方法和系统
CN116515617B (zh) * 2023-07-03 2023-10-20 杭州杰毅生物技术有限公司 一种离心式pcr-荧光多靶标检测芯片

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
US3817837A (en) 1971-05-14 1974-06-18 Syva Corp Enzyme amplification assay
US3939350A (en) 1974-04-29 1976-02-17 Board Of Trustees Of The Leland Stanford Junior University Fluorescent immunoassay employing total reflection for activation
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4277437A (en) 1978-04-05 1981-07-07 Syva Company Kit for carrying out chemically induced fluorescence immunoassay
US4275149A (en) 1978-11-24 1981-06-23 Syva Company Macromolecular environment control in specific receptor assays
US4366241A (en) 1980-08-07 1982-12-28 Syva Company Concentrating zone method in heterogeneous immunoassays
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
ATE198511T1 (de) 1994-04-29 2001-01-15 Perkin Elmer Corp Verfahren und vorrichtung zur echtzeiterfassung der produkte von nukleinsäureamplifikation
ATE196322T1 (de) 1995-05-05 2000-09-15 Perkin Elmer Corp Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay
JP2002515738A (ja) 1996-01-23 2002-05-28 アフィメトリックス,インコーポレイティド 核酸分析法
US5716784A (en) 1996-02-05 1998-02-10 The Perkin-Elmer Corporation Fluorescence detection assay for homogeneous PCR hybridization systems
EP0951569A2 (de) * 1996-12-23 1999-10-27 University Of Chicago Kundengerichtete oligonukleotidchips als vielfaeltige biosensoren
AU1926899A (en) 1997-12-19 1999-07-12 Affymetrix, Inc. Exploiting genomics in the search for new drugs
US5952202A (en) 1998-03-26 1999-09-14 The Perkin Elmer Corporation Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification
US20020177135A1 (en) * 1999-07-27 2002-11-28 Doung Hau H. Devices and methods for biochip multiplexing
DE19949735A1 (de) 1999-10-15 2001-05-10 Bruker Daltonik Gmbh Prozessieren von Proben in Lösungen mit definiert kleiner Wandkontaktfläche
US6316200B1 (en) * 2000-06-08 2001-11-13 Becton, Dickinson And Company Probes and methods for detection of nucleic acids
AU6834700A (en) * 1999-08-13 2001-03-13 Zeptosens Ag Device and method for determining multiple analytes
US7205104B2 (en) 2000-03-24 2007-04-17 Eppendorf Array Technologies Sa (Eat) Identification of biological (micro) organisms by detection of their homologous nucleotide sequences on arrays
JP3963422B2 (ja) * 2000-08-03 2007-08-22 日鉄環境エンジニアリング株式会社 核酸の測定方法
EP1184349A1 (de) 2000-09-01 2002-03-06 A.S.B.L. Facultes Universitaires Notre-Dame De La Paix Verfahren zur Erhaltung einer Oberflächenaktivierung eines festen Trägers zur Bildung von Biochips Mikroanordnungen
US6754414B2 (en) * 2001-09-27 2004-06-22 Bio-Rad Laboratories, Inc. Imaging of microarrays using fiber optic exciter
MXPA04006861A (es) * 2002-01-18 2005-06-20 Univ Utah Res Found Deteccion de polimorfismos de nucleotidos individuales utilizando guias de onda planas.
AU2003233451A1 (en) * 2002-03-26 2003-10-13 Massachusetts Institute Of Technology Targets, methods, and reagents for diagnosis and treatment of schizophrenia
DE10315074A1 (de) * 2003-04-02 2004-10-14 Clondiag Chip Technologies Gmbh Vorrichtung zur Vervielfältigung und zum Nachweis von Nukleinsäuren
JP4074829B2 (ja) * 2003-04-16 2008-04-16 大日本印刷株式会社 バイオマイクロアレイ用基板およびバイオマイクロアレイ
US7622296B2 (en) * 2004-05-28 2009-11-24 Wafergen, Inc. Apparatus and method for multiplex analysis
US20060088844A1 (en) * 2004-10-22 2006-04-27 Honeywell International Inc. Real-time PCR microarray based on evanescent wave biosensor

Also Published As

Publication number Publication date
DE602005023705D1 (de) 2010-11-04
EP1812594A1 (de) 2007-08-01
ATE505560T1 (de) 2011-04-15
WO2006053769A1 (en) 2006-05-26
JP5026978B2 (ja) 2012-09-19
JP2009530589A (ja) 2009-08-27
ATE549420T1 (de) 2012-03-15
EP2302072A1 (de) 2011-03-30
EP1704258A1 (de) 2006-09-27
WO2006053770A8 (en) 2006-08-24
WO2006053770A1 (en) 2006-05-26
US20090191618A1 (en) 2009-07-30
EP2302072B1 (de) 2012-03-14
EP1812594B1 (de) 2013-01-09
EP1704258B1 (de) 2010-09-22

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