ATE549420T1 - Echtzeit-pcr von nukleinsäuren auf einem mikroarray - Google Patents
Echtzeit-pcr von nukleinsäuren auf einem mikroarrayInfo
- Publication number
- ATE549420T1 ATE549420T1 AT10172182T AT10172182T ATE549420T1 AT E549420 T1 ATE549420 T1 AT E549420T1 AT 10172182 T AT10172182 T AT 10172182T AT 10172182 T AT10172182 T AT 10172182T AT E549420 T1 ATE549420 T1 AT E549420T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleotide molecule
- target nucleotide
- molecule
- solution
- labelled target
- Prior art date
Links
- 238000002493 microarray Methods 0.000 title abstract 3
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- 238000003753 real-time PCR Methods 0.000 title 1
- 239000002773 nucleotide Substances 0.000 abstract 10
- 125000003729 nucleotide group Chemical group 0.000 abstract 10
- 238000012408 PCR amplification Methods 0.000 abstract 2
- 230000003321 amplification Effects 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 230000005284 excitation Effects 0.000 abstract 1
- 238000002372 labelling Methods 0.000 abstract 1
- 238000005259 measurement Methods 0.000 abstract 1
- 238000012544 monitoring process Methods 0.000 abstract 1
- 230000009870 specific binding Effects 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0636—Integrated biosensor, microarrays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0822—Slides
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Pathology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Clinical Laboratory Science (AREA)
- General Engineering & Computer Science (AREA)
- Optics & Photonics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Radiation-Therapy Devices (AREA)
- Radio Relay Systems (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/991,087 US8288128B2 (en) | 2004-11-18 | 2004-11-18 | Real-time quantification of multiple targets on a micro-array |
| EP04027435A EP1659183A1 (de) | 2004-11-18 | 2004-11-18 | Quantifizierung einer Vielzahl von Zielmolekülen auf einem Mikroarray in real-time |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE549420T1 true ATE549420T1 (de) | 2012-03-15 |
Family
ID=35539286
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT10172182T ATE549420T1 (de) | 2004-11-18 | 2005-11-18 | Echtzeit-pcr von nukleinsäuren auf einem mikroarray |
| AT05813624T ATE482288T1 (de) | 2004-11-18 | 2005-11-18 | Echtzeit-pcr von zielen auf einem mikroarray |
| AT06114103T ATE505560T1 (de) | 2004-11-18 | 2006-05-17 | Reaktionsgefäss zur real-time pcr umfassend oligonukleotidproben wobei die amplifikationsprodukte durch hybridisierung und ohne öffnen des reaktionsgefässes gemessen werden |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT05813624T ATE482288T1 (de) | 2004-11-18 | 2005-11-18 | Echtzeit-pcr von zielen auf einem mikroarray |
| AT06114103T ATE505560T1 (de) | 2004-11-18 | 2006-05-17 | Reaktionsgefäss zur real-time pcr umfassend oligonukleotidproben wobei die amplifikationsprodukte durch hybridisierung und ohne öffnen des reaktionsgefässes gemessen werden |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20090191618A1 (de) |
| EP (3) | EP1812594B1 (de) |
| JP (1) | JP5026978B2 (de) |
| AT (3) | ATE549420T1 (de) |
| DE (1) | DE602005023705D1 (de) |
| WO (2) | WO2006053769A1 (de) |
Families Citing this family (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7829313B2 (en) | 2000-03-24 | 2010-11-09 | Eppendorf Array Technologies | Identification and quantification of a plurality of biological (micro)organisms or their components |
| US7875442B2 (en) | 2000-03-24 | 2011-01-25 | Eppendorf Array Technologies | Identification and quantification of a plurality of biological (micro)organisms or their components |
| CA2348042A1 (en) | 2001-06-04 | 2002-12-04 | Ann Huletsky | Sequences for detection and identification of methicillin-resistant staphylococcus aureus |
| US11834720B2 (en) | 2005-10-11 | 2023-12-05 | Geneohm Sciences, Inc. | Sequences for detection and identification of methicillin-resistant Staphylococcus aureus (MRSA) of MREJ types xi to xx |
| AU2007251545A1 (en) * | 2006-05-17 | 2007-11-22 | Eppendorf Array Technologies S.A. | Identification and quantification of a plurality of biological (micro)organisms or their components |
| US8637436B2 (en) | 2006-08-24 | 2014-01-28 | California Institute Of Technology | Integrated semiconductor bioarray |
| US11001881B2 (en) | 2006-08-24 | 2021-05-11 | California Institute Of Technology | Methods for detecting analytes |
| WO2007143669A2 (en) | 2006-06-05 | 2007-12-13 | California Institute Of Technology | Real time micro arrays |
| US11525156B2 (en) | 2006-07-28 | 2022-12-13 | California Institute Of Technology | Multiplex Q-PCR arrays |
| US8048626B2 (en) | 2006-07-28 | 2011-11-01 | California Institute Of Technology | Multiplex Q-PCR arrays |
| US11560588B2 (en) | 2006-08-24 | 2023-01-24 | California Institute Of Technology | Multiplex Q-PCR arrays |
| JP2008102117A (ja) * | 2006-09-21 | 2008-05-01 | Fujifilm Corp | 表面プラズモン増強蛍光センサおよび蛍光検出方法 |
| EP1935496A1 (de) * | 2006-12-22 | 2008-06-25 | Eppendorf Array Technologies SA | Vorrichtung und/oder Verfahren zur Detektion erweiterter Nukleotidsequenzen auf Mikro-Arrays |
| EP1946841A1 (de) * | 2006-12-22 | 2008-07-23 | Eppendorf Array Technologies SA | Vorrichtung und/oder Verfahren zum Nachweis von amplifizierten Nukleotidsequenzen auf Mikroarrays |
| JP2009060859A (ja) * | 2007-09-07 | 2009-03-26 | Sumitomo Bakelite Co Ltd | 遺伝子の検出方法 |
| US7914734B2 (en) * | 2007-12-19 | 2011-03-29 | Singulex, Inc. | Scanning analyzer for single molecule detection and methods of use |
| JP5309160B2 (ja) | 2008-02-05 | 2013-10-09 | ポカード・ディアグノスティクス・リミテッド | 生体サンプル中のバクテリアの同定を行うシステム |
| EP2107125A1 (de) | 2008-03-31 | 2009-10-07 | Eppendorf Array Technologies SA (EAT) | Echtzeit-PCR von Targets auf einem Mikroarray |
| US20100069253A1 (en) * | 2008-09-12 | 2010-03-18 | Gindilis Andrei L | Impedance Spectroscopy Measurement of DNA |
| EP2382324A1 (de) | 2008-12-30 | 2011-11-02 | STMicroelectronics S.r.l. | Verfahren, mikroreaktor und vorrichtung zur durchführung von nukleinsäureamplifikation in echtzeit |
| WO2010084381A1 (en) * | 2009-01-21 | 2010-07-29 | Eppendorf Ag | Detection and/or quantification method of target molecules on a solid support |
| EP2221605A1 (de) * | 2009-02-12 | 2010-08-25 | Koninklijke Philips Electronics N.V. | Drahtgittersensor |
| US10288632B2 (en) | 2009-09-21 | 2019-05-14 | Pocared Diagnostics Ltd. | System for conducting the identification of bacteria in biological samples |
| EP2369325A1 (de) | 2010-03-12 | 2011-09-28 | Eppendorf Ag | Arrayanalyse für Online-Detektion |
| EP2441520A1 (de) | 2010-10-12 | 2012-04-18 | Eppendorf AG | Echtzeitverstärkungs- und Mikroarray-basierte Erkennung von Nukleinsäuretargets in einem Fluss-Chip-Assay |
| EP2651560B1 (de) | 2010-12-17 | 2020-03-18 | BJS IP Limited | Verfahren und systeme für schnelles pcr-erwärmen |
| CN103946690B (zh) * | 2011-11-28 | 2017-03-15 | 索尼公司 | 化学传感器、化学传感器模块、化学物检测装置和化学物检测方法 |
| KR20150010939A (ko) * | 2012-03-16 | 2015-01-29 | 라이프 테크놀로지스 코포레이션 | 생물학적 샘플을 평가하기 위한 시스템 및 방법 |
| US9579657B2 (en) | 2012-05-24 | 2017-02-28 | Bjs Ip Ltd | Clamp for fast PCR heating |
| CN104237544B (zh) * | 2014-09-28 | 2016-05-11 | 博奥生物集团有限公司 | 一种生物芯片检测器 |
| US9708647B2 (en) | 2015-03-23 | 2017-07-18 | Insilixa, Inc. | Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays |
| US9499861B1 (en) | 2015-09-10 | 2016-11-22 | Insilixa, Inc. | Methods and systems for multiplex quantitative nucleic acid amplification |
| KR102478639B1 (ko) | 2016-02-23 | 2022-12-19 | 노을 주식회사 | 표지 물질을 저장하는 패치, 이를 이용하는 조직 진단 방법 및 장치 |
| KR102045068B1 (ko) * | 2016-02-23 | 2019-11-15 | 노을 주식회사 | 중합 효소 연쇄 반응 패치, 이를 이용하는 진단 방법 및 장치 |
| US10371610B2 (en) | 2016-02-23 | 2019-08-06 | Noul Co., Ltd. | Contact-type patch, staining method using the same, and manufacturing method thereof |
| KR20170099738A (ko) | 2016-02-23 | 2017-09-01 | 노을 주식회사 | 접촉식 염색 패치 및 그 제조 방법 |
| KR102478636B1 (ko) * | 2016-02-23 | 2022-12-19 | 노을 주식회사 | 중합 효소 연쇄 반응 패치, 이를 이용하는 진단 방법 및 장치 |
| WO2017155858A1 (en) | 2016-03-07 | 2017-09-14 | Insilixa, Inc. | Nucleic acid sequence identification using solid-phase cyclic single base extension |
| CN119510370A (zh) | 2019-03-14 | 2025-02-25 | 因斯利克萨公司 | 基于时间门控的荧光检测的方法和系统 |
| CN116515617B (zh) * | 2023-07-03 | 2023-10-20 | 杭州杰毅生物技术有限公司 | 一种离心式pcr-荧光多靶标检测芯片 |
Family Cites Families (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL154598B (nl) | 1970-11-10 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking. |
| US3817837A (en) | 1971-05-14 | 1974-06-18 | Syva Corp | Enzyme amplification assay |
| US3939350A (en) | 1974-04-29 | 1976-02-17 | Board Of Trustees Of The Leland Stanford Junior University | Fluorescent immunoassay employing total reflection for activation |
| US3996345A (en) | 1974-08-12 | 1976-12-07 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
| US4275149A (en) | 1978-11-24 | 1981-06-23 | Syva Company | Macromolecular environment control in specific receptor assays |
| US4277437A (en) | 1978-04-05 | 1981-07-07 | Syva Company | Kit for carrying out chemically induced fluorescence immunoassay |
| US4366241A (en) | 1980-08-07 | 1982-12-28 | Syva Company | Concentrating zone method in heterogeneous immunoassays |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| US5994056A (en) | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| EP0706649B1 (de) | 1994-04-29 | 2001-01-03 | Perkin-Elmer Corporation | Verfahren und vorrichtung zur echtzeiterfassung der produkte von nukleinsäureamplifikation |
| ATE196322T1 (de) | 1995-05-05 | 2000-09-15 | Perkin Elmer Corp | Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay |
| JP2002515738A (ja) | 1996-01-23 | 2002-05-28 | アフィメトリックス,インコーポレイティド | 核酸分析法 |
| US5716784A (en) | 1996-02-05 | 1998-02-10 | The Perkin-Elmer Corporation | Fluorescence detection assay for homogeneous PCR hybridization systems |
| EP0951569A2 (de) * | 1996-12-23 | 1999-10-27 | University Of Chicago | Kundengerichtete oligonukleotidchips als vielfaeltige biosensoren |
| DE69822206T2 (de) | 1997-12-19 | 2005-02-17 | Affymetrix, Inc., Santa Clara | Erkenntnisse der genomforschung für die suche nach neuartigen wirkstoffen |
| US5952202A (en) | 1998-03-26 | 1999-09-14 | The Perkin Elmer Corporation | Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification |
| US20020177135A1 (en) * | 1999-07-27 | 2002-11-28 | Doung Hau H. | Devices and methods for biochip multiplexing |
| DE19949735A1 (de) | 1999-10-15 | 2001-05-10 | Bruker Daltonik Gmbh | Prozessieren von Proben in Lösungen mit definiert kleiner Wandkontaktfläche |
| US6316200B1 (en) * | 2000-06-08 | 2001-11-13 | Becton, Dickinson And Company | Probes and methods for detection of nucleic acids |
| AU6834700A (en) * | 1999-08-13 | 2001-03-13 | Zeptosens Ag | Device and method for determining multiple analytes |
| US7205104B2 (en) | 2000-03-24 | 2007-04-17 | Eppendorf Array Technologies Sa (Eat) | Identification of biological (micro) organisms by detection of their homologous nucleotide sequences on arrays |
| JP3963422B2 (ja) * | 2000-08-03 | 2007-08-22 | 日鉄環境エンジニアリング株式会社 | 核酸の測定方法 |
| EP1184349A1 (de) | 2000-09-01 | 2002-03-06 | A.S.B.L. Facultes Universitaires Notre-Dame De La Paix | Verfahren zur Erhaltung einer Oberflächenaktivierung eines festen Trägers zur Bildung von Biochips Mikroanordnungen |
| US6754414B2 (en) * | 2001-09-27 | 2004-06-22 | Bio-Rad Laboratories, Inc. | Imaging of microarrays using fiber optic exciter |
| AU2003210571A1 (en) * | 2002-01-18 | 2003-09-02 | University Of Utah Research Foundation | Detection of single nucleotide polymorphisms using planar waveguides |
| JP4544865B2 (ja) * | 2002-03-26 | 2010-09-15 | マサチューセッツ インスティテュート オブ テクノロジー | 統合失調症の診断および治療のための標的、方法、ならびに試薬 |
| DE10315074A1 (de) * | 2003-04-02 | 2004-10-14 | Clondiag Chip Technologies Gmbh | Vorrichtung zur Vervielfältigung und zum Nachweis von Nukleinsäuren |
| JP4074829B2 (ja) * | 2003-04-16 | 2008-04-16 | 大日本印刷株式会社 | バイオマイクロアレイ用基板およびバイオマイクロアレイ |
| US7311794B2 (en) * | 2004-05-28 | 2007-12-25 | Wafergen, Inc. | Methods of sealing micro wells |
| US20060088844A1 (en) * | 2004-10-22 | 2006-04-27 | Honeywell International Inc. | Real-time PCR microarray based on evanescent wave biosensor |
-
2005
- 2005-11-18 AT AT10172182T patent/ATE549420T1/de active
- 2005-11-18 DE DE602005023705T patent/DE602005023705D1/de not_active Expired - Lifetime
- 2005-11-18 AT AT05813624T patent/ATE482288T1/de not_active IP Right Cessation
- 2005-11-18 WO PCT/EP2005/012382 patent/WO2006053769A1/en not_active Ceased
- 2005-11-18 EP EP05806579A patent/EP1812594B1/de not_active Expired - Lifetime
- 2005-11-18 EP EP10172182A patent/EP2302072B1/de not_active Revoked
- 2005-11-18 EP EP05813624A patent/EP1704258B1/de not_active Expired - Lifetime
- 2005-11-18 JP JP2007541801A patent/JP5026978B2/ja not_active Expired - Fee Related
- 2005-11-18 WO PCT/EP2005/012383 patent/WO2006053770A1/en not_active Ceased
-
2006
- 2006-05-17 AT AT06114103T patent/ATE505560T1/de not_active IP Right Cessation
-
2007
- 2007-05-14 US US12/301,186 patent/US20090191618A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| ATE505560T1 (de) | 2011-04-15 |
| WO2006053769A1 (en) | 2006-05-26 |
| EP1812594A1 (de) | 2007-08-01 |
| US20090191618A1 (en) | 2009-07-30 |
| EP1812594B1 (de) | 2013-01-09 |
| ATE482288T1 (de) | 2010-10-15 |
| EP2302072B1 (de) | 2012-03-14 |
| EP1704258A1 (de) | 2006-09-27 |
| JP2009530589A (ja) | 2009-08-27 |
| EP2302072A1 (de) | 2011-03-30 |
| WO2006053770A1 (en) | 2006-05-26 |
| JP5026978B2 (ja) | 2012-09-19 |
| EP1704258B1 (de) | 2010-09-22 |
| WO2006053770A8 (en) | 2006-08-24 |
| DE602005023705D1 (de) | 2010-11-04 |
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