CN107326085B - 多路生化测定中信号的编码和解码 - Google Patents

多路生化测定中信号的编码和解码 Download PDF

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CN107326085B
CN107326085B CN201710674587.8A CN201710674587A CN107326085B CN 107326085 B CN107326085 B CN 107326085B CN 201710674587 A CN201710674587 A CN 201710674587A CN 107326085 B CN107326085 B CN 107326085B
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CN107326085A (zh
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埃米尔·P·卡尔塔洛夫
阿迪蒂亚·拉贾戈帕
阿克塞尔·谢勒
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California Institute of Technology
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CN201710674587.8A 2012-02-03 2013-02-01 多路生化测定中信号的编码和解码 Active CN107326085B (zh)

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US201261594480P 2012-02-03 2012-02-03
US61/594,480 2012-02-03
US201261703093P 2012-09-19 2012-09-19
US61/703,093 2012-09-19
CN201380018760.9A CN104271767B (zh) 2012-02-03 2013-02-01 多路生化测定中信号的编码和解码
PCT/US2013/024509 WO2013116780A1 (en) 2012-02-03 2013-02-01 Signal encoding and decoding in multiplexed biochemical assays

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CN107326085B true CN107326085B (zh) 2022-05-03

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US (10) US8838394B2 (enExample)
EP (6) EP2809813B1 (enExample)
JP (1) JP6255353B2 (enExample)
KR (2) KR101912401B1 (enExample)
CN (3) CN107326085B (enExample)
BR (1) BR112014019168B1 (enExample)
WO (1) WO2013116780A1 (enExample)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112014019168B1 (pt) 2012-02-03 2021-10-05 California Institute Of Technology Método capaz de detectar de forma não degenerada presença ou ausência de analitos em um único volume de amostra e método de detecção da presença ou ausência de cada analito dentre uma pluralidade de analitos
CN104662172B (zh) 2012-08-03 2018-07-06 加州理工学院 Pcr中具有减少的硬件和要求的多重化和定量
EP4570924A3 (en) 2013-01-24 2025-09-03 California Institute of Technology Chromophore-based characterization and detection methods
US10077475B2 (en) 2013-01-24 2018-09-18 California Institute Of Technology FRET-based analytes detection and related methods and systems
KR101789079B1 (ko) 2014-09-22 2017-10-24 건국대학교 산학협력단 마이크로알엔에이 검지를 위한 핵산 다중 접합체 및 그 제조방법
WO2016120396A1 (en) * 2015-01-29 2016-08-04 Instituut Voor Tropische Geneeskunde Combined plasmodium-rna virus detection method
US10429294B2 (en) 2015-04-02 2019-10-01 University Of Washington Optical painting and fluorescence-activated sorting of adherent cells
WO2017112025A2 (en) 2015-10-05 2017-06-29 The University Of North Carolina At Chapel Hill Decoding methods for multiplexing assays and associated fluidic devices, kits, and solid supports
WO2017173035A1 (en) 2016-04-01 2017-10-05 Chromacode Inc. Competitive probes for engineering signal generation
CN109923613B (zh) * 2016-06-02 2023-07-04 Seegene株式会社 利用信号变化量数据集的样品内的目标分析物质检测方法
US10066263B2 (en) 2016-06-17 2018-09-04 California Institute Of Technology Nucleic acid reactions and related methods and compositions
JP6846636B2 (ja) * 2017-02-28 2021-03-24 パナソニックIpマネジメント株式会社 核酸検出用オリゴヌクレオチド
EP3645749B1 (en) * 2017-06-28 2022-03-02 Chromacode, Inc. Multiplexed fluorometric measurements with droplet pcr systems
JP2019154246A (ja) * 2018-03-08 2019-09-19 株式会社リコー プローブセット、及びそれを用いた核酸検出方法
CN112424377A (zh) 2018-04-17 2021-02-26 克罗玛科德公司 用于多重分析的方法和系统
WO2019236447A1 (en) * 2018-06-04 2019-12-12 ChromaCode, Inc. Methods for watermarking and identifying chemical compositions
US12203129B2 (en) * 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
CN112805392A (zh) * 2018-07-11 2021-05-14 克罗玛科德公司 多重生化反应中分析物的定量方法
USD975312S1 (en) 2020-02-14 2023-01-10 Beckman Coulter, Inc. Reagent cartridge
CN115851890A (zh) * 2022-10-08 2023-03-28 江苏圣极基因科技有限公司 基于数字pcr技术的多重核酸定量检测方法
KR20250159770A (ko) * 2024-05-03 2025-11-11 대한민국(질병관리청장) 호흡기증후군 원인병원체 동시 검출용 병원체 특이 프로브 세트 및 이의 용도
WO2025235889A1 (en) * 2024-05-10 2025-11-13 Guardant Health, Inc. Methods involving multiplexed pooled pcr
WO2026019326A1 (en) 2024-07-18 2026-01-22 Biomiris Capital Group B.V. Probe-resolved melting curve analysis for identifying microorganisms in clinical samples

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030148544A1 (en) * 2001-06-28 2003-08-07 Advanced Research And Technology Institute, Inc. Methods of preparing multicolor quantum dot tagged beads and conjugates thereof
CN1936019A (zh) * 2005-10-18 2007-03-28 厦门大学 用于多重实时核酸扩增检测的探针编码方法

Family Cites Families (158)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0232967B1 (en) 1986-01-10 1993-04-28 Amoco Corporation Competitive homogeneous assay
US5618711A (en) 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US6127155A (en) 1986-08-22 2000-10-03 Roche Molecular Systems, Inc. Stabilized thermostable nucleic acid polymerase compositions containing non-ionic polymeric detergents
US5079351A (en) 1986-11-26 1992-01-07 Cetus Corporation Oligonucleotides and kits for detection of htlvi and htlvii viruses by hybridization
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
EP0519338B1 (en) 1991-06-20 1996-08-28 F. Hoffmann-La Roche Ag Improved methods for nucleic acid amplification
EP0675966B1 (en) 1992-02-19 2004-10-06 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5948360A (en) * 1994-07-11 1999-09-07 Tekmar Company Autosampler with robot arm
CN1100258C (zh) 1995-02-02 2003-01-29 中外制药株式会社 以调节化学发光为手段分析被分析物的方法
KR19990008052A (ko) 1995-04-25 1999-01-25 마이클 피 노바 기억 장치를 갖는 원격적으로 프로그램 가능한 매트릭스 및 이의 용도
ATE196322T1 (de) 1995-05-05 2000-09-15 Perkin Elmer Corp Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay
US5882856A (en) 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US20050112637A1 (en) 1995-09-08 2005-05-26 Chatterjee Deb K. Mutant DNA polymerases and uses thereof
US5981180A (en) 1995-10-11 1999-11-09 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
US6007994A (en) 1995-12-22 1999-12-28 Yale University Multiparametric fluorescence in situ hybridization
EP0929694A4 (en) 1996-03-15 2002-05-02 Penn State Res Found DETECTION OF TUMOR-RELATED EXTRACELLULAR NUCLEIC ACID USING PLASMA OR BLOOD SERUM USING NUCLEIC ACID AMPLIFICATION TESTS
PT938320E (pt) 1996-03-26 2010-09-22 Michael S Kopreski Método que permite a utilização de arn extracelular extraído de plasma ou de soro para detectar, monitorizar ou avaliar o cancro
US6174670B1 (en) 1996-06-04 2001-01-16 University Of Utah Research Foundation Monitoring amplification of DNA during PCR
US5834203A (en) 1997-08-25 1998-11-10 Applied Spectral Imaging Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardwire therefore
US6632526B1 (en) 1997-10-14 2003-10-14 Luminex Corporation Precision fluorescently dyed particles and methods of making and using same
US6235480B1 (en) 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
AU3555599A (en) 1998-04-13 1999-11-01 Luminex Corporation Liquid labeling with fluorescent microparticles
DE69940430D1 (de) * 1998-06-24 2009-04-02 Illumina Inc Dekodierung von matrixartig-angeordneten Sensoren durch Mikropartikel
AU762728B2 (en) 1998-07-02 2003-07-03 Gen-Probe Incorporated Molecular torches
US6642062B2 (en) 1998-09-03 2003-11-04 Trellis Bioinformatics, Inc. Multihued labels
JP2002542793A (ja) 1999-04-22 2002-12-17 ザ アルバート アインシュタイン カレッジ オブ メディシン オブ イエシバ ユニバーシティ 多蛍光fishによる遺伝子発現パターンのアッセイ法
DE60035691T2 (de) 1999-07-23 2008-04-30 Forensic Science Service Ltd. Verfahren zur Erkennung von Einzel-Nukleotid-Polymorphismen
DE60030145T2 (de) 1999-10-22 2008-01-10 PHRI Properties, Inc., Newark Nachweisverfahren für kurze sequenzvarianten
US20040053230A1 (en) 2001-03-07 2004-03-18 Schaffer Michael Edward Real time quantitative pcr with intercalating dye for single and multiplex target dna
DE60143974D1 (de) 2000-03-29 2011-03-10 Lgc Ltd Hybridisierungsprobe und Methode zum schnellen Nachweis und zur schnellen Unterscheidung von Sequenzen
US6734420B2 (en) 2000-04-06 2004-05-11 Quantum Dot Corporation Differentiable spectral bar code methods and systems
US20030064366A1 (en) * 2000-07-07 2003-04-03 Susan Hardin Real-time sequence determination
US7608683B2 (en) * 2000-08-09 2009-10-27 The Regents Of The University Of California Stress proteins and peptides and methods of use thereof
CA2314398A1 (en) * 2000-08-10 2002-02-10 Edward Shipwash Microarrays and microsystems for amino acid analysis and protein sequencing
US20020128228A1 (en) * 2000-12-01 2002-09-12 Wen-Jen Hwu Compositions and methods for the treatment of cancer
US20020142345A1 (en) 2000-12-22 2002-10-03 Nelsen Anita J. Methods for encoding and decoding complex mixtures in arrayed assays
AU2002324422A1 (en) * 2001-02-21 2002-12-23 Amnis Corporation Method and apparatus for labeling and analyzing cellular components
CA2439402A1 (en) 2001-03-02 2002-09-12 University Of Pittsburgh Of The Commonwealth System Of Higher Education Pcr method
WO2003002979A2 (en) * 2001-06-28 2003-01-09 Illumina, Inc. Multiplex decoding of array sensors with microspheres
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
EP1288308A1 (en) 2001-08-28 2003-03-05 Roche Diagnostics GmbH A method for the determination of multiple analytes
US6852491B2 (en) 2001-09-04 2005-02-08 Abbott Laboratories Amplification and detection reagents for HIV-1
US20030134320A1 (en) 2002-01-15 2003-07-17 Myriad Genetics, Incorporated Method system and computer program product for quality assurance in detecting biochemical markers
EP1426448A1 (en) 2002-12-03 2004-06-09 bioMérieux BV Method for lowering the effects of sequence variations in a diagnostic hybridization assay, probe for use in the assay and assay
WO2004087950A2 (en) 2003-04-04 2004-10-14 Roche Diagnostics Gmbh Improved system for multi color real time pcr
US7385043B1 (en) 2003-04-30 2008-06-10 The Public Health Research Institute Of The City Of New York, Inc. Homogeneous multiplex screening assays and kits
US7070786B2 (en) 2003-06-06 2006-07-04 Centocor, Inc. RSV proteins, antibodies, compositions, methods and uses
US20050053950A1 (en) 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
US20050064435A1 (en) 2003-09-24 2005-03-24 Xing Su Programmable molecular barcodes
TWI232934B (en) 2003-11-19 2005-05-21 Ind Tech Res Inst A biochip containing splitable reaction confinement and method for producing same and application thereof
JP2007534308A (ja) 2003-11-19 2007-11-29 アレロジック・バイオサイエンシズ・コーポレーション 複数のフルオロフォアで標識したオリゴヌクレオチド
ATE431433T1 (de) * 2004-03-24 2009-05-15 Applied Biosystems Llc Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen
AU2005230833B2 (en) 2004-04-01 2011-04-14 Bio-Rad Laboratories, Inc. Quantitative amplification with a labeled probe and 3' to 5' exonuclease activity
ES2527426T3 (es) 2004-05-07 2015-01-23 Cepheid Detección combinada de agentes biológicos
US7575864B2 (en) 2004-05-27 2009-08-18 E.I. Du Pont De Nemours And Company Method for the direct detection of diagnostic RNA
DE102004043870B4 (de) 2004-09-10 2009-11-19 Eppendorf Ag Verfahren zur Erweiterung des dynamischen Erfassungsbereich bei Mikroarrays
US7671184B2 (en) 2004-09-24 2010-03-02 Universitact Bern Molecular beacons
AU2005295298B2 (en) 2004-10-18 2011-07-14 Brandeis University Primers, probes and methods for nucleic acid amplification
CA2587085C (en) 2004-10-27 2017-08-08 Cepheid Closed-system multi-stage nucleic acid amplification reactions
US20060246475A1 (en) 2005-01-21 2006-11-02 Third Wave Technologies Compositions and methods for increased dynamic range detection of nucleic acids
US7951078B2 (en) * 2005-02-03 2011-05-31 Maren Theresa Scheuner Method and apparatus for determining familial risk of disease
US8168602B2 (en) * 2005-02-17 2012-05-01 Dana-Farber Cancer Institute, Inc. Compositions, kits and methods for identification, assessment, prevention and therapy of cancer
US7709249B2 (en) 2005-04-01 2010-05-04 3M Innovative Properties Company Multiplex fluorescence detection device having fiber bundle coupling multiple optical modules to a common detector
US7507575B2 (en) 2005-04-01 2009-03-24 3M Innovative Properties Company Multiplex fluorescence detection device having removable optical modules
US7919244B2 (en) 2005-06-16 2011-04-05 Biotools Biotechnological & Medical Laboratories, S.A. Nucleic acid detection method involving the direct generation of a measurable signal
US8067208B2 (en) 2005-06-30 2011-11-29 Roche Molecular Systems, Inc. Probes and methods for hepatitis C virus typing using multidimensional probe analysis
US7553955B2 (en) 2005-09-01 2009-06-30 The Trustees Of The University Of Pennsylvania Molecular beacons, methods and kits for detecting DNA damage response
JP2009506759A (ja) 2005-09-01 2009-02-19 コーベット ライフ サイエンス ピーティーワイ リミテッド 核酸の増幅、定量化、及び同定の方法。
JP5537034B2 (ja) 2005-12-23 2014-07-02 ナノストリング テクノロジーズ,インコーポレーテッド ナノレポーターならびにその作製方法および使用方法
US8986926B2 (en) 2005-12-23 2015-03-24 Nanostring Technologies, Inc. Compositions comprising oriented, immobilized macromolecules and methods for their preparation
WO2007080583A2 (en) 2006-01-10 2007-07-19 Applied Spectral Imaging Ltd. Methods and systems for analyzing biological samples
US8455184B2 (en) 2006-04-18 2013-06-04 The United States Of America As Represented By The Secretary Of The Air Force Differential multiplexing with pattern recognition
JP2009538123A (ja) 2006-04-19 2009-11-05 アプライド バイオシステムズ, エルエルシー ゲル非含有ビーズベースの配列決定のための試薬、方法およびライブラリー
ES2620398T3 (es) 2006-05-22 2017-06-28 Nanostring Technologies, Inc. Sistemas y métodos para analizar nanoindicadores
WO2007137291A1 (en) 2006-05-23 2007-11-29 Molecular Detection, Inc. Ambient temperature stable kits for molecular diagnostics
EP2041318A2 (en) 2006-06-26 2009-04-01 Blood Cell Storage, Inc. Device and method for extraction and analysis of nucleic acids from biological samples
WO2008005248A2 (en) * 2006-06-30 2008-01-10 Canon U.S. Life Sciences, Inc. Real-time pcr in micro-channels
US8048626B2 (en) 2006-07-28 2011-11-01 California Institute Of Technology Multiplex Q-PCR arrays
US8187541B2 (en) * 2006-09-18 2012-05-29 California Institute Of Technology Apparatus for detecting target molecules and related methods
WO2008054595A2 (en) 2006-09-28 2008-05-08 The Regents Of The University Of California Drug controlled molecular tags
WO2008052774A2 (en) 2006-10-31 2008-05-08 Noxxon Pharma Ag Methods for detection of a single- or double-stranded nucleic acid molecule
US9201063B2 (en) 2006-11-16 2015-12-01 General Electric Company Sequential analysis of biological samples
WO2008067567A2 (en) 2006-11-30 2008-06-05 Gen-Probe Incorporated Quantitative method employing adjustment of pre-defined master calibration curves
KR101433208B1 (ko) 2006-12-13 2014-08-22 루미넥스 코포레이션 실시간으로 pcr의 다중 분석을 위한 시스템과 방법
CA2580589C (en) * 2006-12-19 2016-08-09 Fio Corporation Microfluidic detection system
WO2008109636A1 (en) 2007-03-05 2008-09-12 University Of Utah Research Foundation Methods and compositions related to nucleic acid detection
WO2008118998A2 (en) 2007-03-27 2008-10-02 Primera Biosystems Inc. Method for multiplex detection and quantitation of nucleic acids
WO2008124847A2 (en) 2007-04-10 2008-10-16 Nanostring Technologies, Inc. Methods and computer systems for identifying target-specific sequences for use in nanoreporters
WO2009003493A2 (en) 2007-07-03 2009-01-08 Dako Denmark A/S Mhc multimers, methods for their generation, labeling and use
EP2193356B1 (en) 2007-08-29 2014-07-23 Eppendorf Ag Device and method for radiometric measurement of a plurality of samples
WO2009036514A2 (en) 2007-09-20 2009-03-26 Molecular Plant Breeding Nominees Ltd Method of amplifying nucleic acid
WO2009081967A1 (ja) 2007-12-26 2009-07-02 Arkray, Inc. 標的核酸配列の増幅方法およびそれに用いるプローブ
EP2116614A1 (en) 2008-05-06 2009-11-11 Qiagen GmbH Simultaneous detection of multiple nucleic acid sequences in a reaction
EA201170088A1 (ru) * 2008-06-25 2011-10-31 Бейлор Рисёч Инститьют Транскрипционная сигнатура крови инфекции mycobacterium tuberculosis
GB0812862D0 (en) 2008-07-14 2008-08-20 Lumora Ltd Nucleic acid amplification
WO2010013017A1 (en) 2008-07-31 2010-02-04 Oxitec Limited Multiplex amplification and detection
EP2315844A1 (en) 2008-08-08 2011-05-04 Smiths Detection Inc. Detection algorithm for pcr assay
US8519115B2 (en) 2008-08-14 2013-08-27 Nanostring Technologies, Inc. Stable nanoreporters
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US9921154B2 (en) 2011-03-18 2018-03-20 Bio-Rad Laboratories, Inc. Multiplexed digital assays
GB0821457D0 (en) * 2008-11-24 2008-12-31 Trillion Genomics Ltd Oligonucleotides
WO2010063732A1 (en) 2008-12-01 2010-06-10 Biotype Ag Novel combination of fluorescent dyes for the detection of nucleic acids
CN102301005A (zh) 2008-12-17 2011-12-28 生命技术公司 用于检测等位基因变体的方法、组合物和试剂盒
US8361738B2 (en) * 2009-03-06 2013-01-29 California Institute Of Technology Methods for quantitative target detection and related devices and systems
US8039215B2 (en) 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
CN101570782B (zh) * 2009-03-20 2012-01-11 曹际娟 乳制品中8种致病菌检测试剂盒及其检测方法
FI20095514A0 (fi) * 2009-05-07 2009-05-07 Expression Analytics Oy Menetelmä, laitteisto ja tietokoneohjelmatuote PCR-tuotteiden kvantifioimiseksi
EP2430187A2 (en) 2009-05-12 2012-03-21 Abacus Diagnostica OY Method for detecting nucleic acids
CN102449166B (zh) 2009-05-26 2013-12-04 厦门大学 一种单管检测多个单核苷酸变异或单核苷酸多态性的方法
US8691504B2 (en) 2009-05-26 2014-04-08 Xiamen University Method for detecting variations in nucleic acid sequences
EP2280080A1 (en) 2009-07-31 2011-02-02 Qiagen GmbH Method of normalized quantification of nucleic acids using anchor oligonucleotides and adapter oligonucleotides
EP3375890B1 (en) 2009-09-10 2020-01-22 DiaSorin S.p.A. Methods and devices for determining a crosstalk correction in nucleic acid amplification
US9447457B2 (en) 2009-09-24 2016-09-20 Seegene, Inc. Detection of target nucleic acid sequences by cyclic exonucleolytic reactions
US8962251B2 (en) 2009-10-08 2015-02-24 Medical Service Consultation International, Llc Methods and compositions for identifying sulfur and iron modifying bacteria
WO2011047087A2 (en) 2009-10-13 2011-04-21 Nanostring Technologies, Inc. Protein detection via nanoreporters
US9752187B2 (en) 2009-12-11 2017-09-05 Nucleix Categorization of DNA samples
US9220743B2 (en) 2010-01-22 2015-12-29 Nuovo Biologics, Llc Pan-antiviral peptides for protein kinase inhibition
WO2011100541A2 (en) 2010-02-11 2011-08-18 Nanostring Technologies, Inc. Compositions and methods for the detection of small rnas
EP4435111A1 (en) 2010-02-12 2024-09-25 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
EP2547795B1 (en) 2010-03-16 2015-01-14 Nanostring Technologies, Inc Compositions and methods for the detection of genomic features
CA2799200A1 (en) 2010-05-19 2011-11-24 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
CN102985566B (zh) 2010-06-30 2015-06-17 简.探针公司 使用分析物和过程控制信号的单次读取确定而鉴定含分析物的样品的方法和设备
US8877464B2 (en) 2010-07-29 2014-11-04 Roche Molecular Systems, Inc. Qualitative and quantitative detection of microbial nucleic acids
US10024796B2 (en) 2010-10-29 2018-07-17 President And Fellows Of Harvard College Nucleic acid nanostructure barcode probes
JP6063395B2 (ja) 2011-01-20 2017-01-18 ユニバーシティ オブ ワシントン スルー イッツ センター フォー コマーシャライゼーション デジタル測定を実施するための方法およびシステム
US20150140554A1 (en) 2011-02-03 2015-05-21 Richard O. Snyder Detection Methods for Target DNA
JP2014509865A (ja) 2011-03-18 2014-04-24 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド シグナルの組合せ使用による多重化デジタルアッセイ
WO2012129115A2 (en) 2011-03-18 2012-09-27 University Of South Florida Materials and methods for profiling micrornas
US9758834B2 (en) 2011-03-28 2017-09-12 Nanostring Technologies, Inc. Compositions and methods for diagnosing cancer
CA2839705A1 (en) 2011-06-24 2012-12-27 Nanostring Technologies, Inc. Multivariate diagnostic assays and methods for using same
US10196681B2 (en) 2011-10-06 2019-02-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US8858623B2 (en) 2011-11-04 2014-10-14 Valtech Cardio, Ltd. Implant having multiple rotational assemblies
CN102559868B (zh) * 2011-11-28 2014-11-05 厦门大学 一种单管定性并定量检测多个目标核酸序列的方法
EP2794928B1 (en) * 2011-12-22 2019-02-20 President and Fellows of Harvard College Compositions and methods for analyte detection
BR112014019168B1 (pt) 2012-02-03 2021-10-05 California Institute Of Technology Método capaz de detectar de forma não degenerada presença ou ausência de analitos em um único volume de amostra e método de detecção da presença ou ausência de cada analito dentre uma pluralidade de analitos
WO2014004609A2 (en) 2012-06-27 2014-01-03 University Of Medicine & Dentistry Of New Jersey Rapid assays for t-cell activation by rna measurements using flow cytometry
CN104662172B (zh) 2012-08-03 2018-07-06 加州理工学院 Pcr中具有减少的硬件和要求的多重化和定量
GB201218909D0 (en) 2012-10-22 2012-12-05 Univ Singapore Assay for the parallel detection of biological material based on PCR
EP4570924A3 (en) 2013-01-24 2025-09-03 California Institute of Technology Chromophore-based characterization and detection methods
CN105339505B (zh) 2013-03-12 2021-08-10 生命技术公司 基于通用报告体的基因分型方法和材料
EP2873740A1 (en) 2013-11-19 2015-05-20 Oncodiag Methods for the surveillance, diagnosis and screening of bladder cancer
US9670530B2 (en) 2014-01-30 2017-06-06 Illumina, Inc. Haplotype resolved genome sequencing
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
US10801067B2 (en) 2014-05-09 2020-10-13 Eurofins Lifecodexx Gmbh Detection of DNA that originates from a specific cell-type and related methods
EP3274476B1 (en) 2015-03-25 2023-06-07 The General Hospital Corporation Digital analysis of circulating tumor cells in blood samples
EP3800249B1 (en) 2016-02-10 2022-06-22 The Regents of The University of Michigan Detection of nucleic acids
WO2017173035A1 (en) 2016-04-01 2017-10-05 Chromacode Inc. Competitive probes for engineering signal generation
US10066263B2 (en) 2016-06-17 2018-09-04 California Institute Of Technology Nucleic acid reactions and related methods and compositions
KR102288461B1 (ko) 2016-10-24 2021-08-11 온테라 인크. 시료 내 폴리뉴클레오타이드 서열의 분포 분율
EP3645749B1 (en) 2017-06-28 2022-03-02 Chromacode, Inc. Multiplexed fluorometric measurements with droplet pcr systems
US20190112636A1 (en) 2017-10-16 2019-04-18 ChromaCode, Inc. Methods and compositions for nucleic acid detection
WO2019169043A1 (en) 2018-02-28 2019-09-06 ChromaCode, Inc. Molecular targets for fetal nucleic acid analysis
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030148544A1 (en) * 2001-06-28 2003-08-07 Advanced Research And Technology Institute, Inc. Methods of preparing multicolor quantum dot tagged beads and conjugates thereof
CN1936019A (zh) * 2005-10-18 2007-03-28 厦门大学 用于多重实时核酸扩增检测的探针编码方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Identification of 8 Foodborne Pathogens by Multicolor Combinational Probe Coding Technology in a Single Real-Time PCR;Qiuying Huang et al.;《Clinical Chemistry》;20071231;第53卷(第10期);第1741-1748页 *
多重连接探针扩增技术及其应用;周大文 等;《中华医学遗传学杂志》;20090228;第26卷(第1期);第45-49页 *

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