CN107099516A - 7β‑羟基甾醇脱氢酶突变体及其在熊脱氧胆酸合成中的应用 - Google Patents
7β‑羟基甾醇脱氢酶突变体及其在熊脱氧胆酸合成中的应用 Download PDFInfo
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P33/00—Preparation of steroids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
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Cited By (9)
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CN108546691A (zh) * | 2018-05-09 | 2018-09-18 | 华东理工大学 | 7β-羟基甾醇脱氢酶突变体及其在制备熊脱氧胆酸中的应用 |
CN109182284A (zh) * | 2018-09-28 | 2019-01-11 | 湖南福来格生物技术有限公司 | 一种7β-羟基类固醇脱氢酶突变体、编码序列、重组表达载体、基因工程菌及应用 |
CN109486738A (zh) * | 2018-12-10 | 2019-03-19 | 江南大学 | 一种利用重组大肠杆菌全细胞转化生产宝丹酮的方法 |
CN109722442A (zh) * | 2017-10-31 | 2019-05-07 | 中国科学院天津工业生物技术研究所 | 7β-羟基胆酸脱氢酶及其应用 |
CN111094557A (zh) * | 2018-02-12 | 2020-05-01 | 江南大学 | 醇脱氢酶突变体及其在双芳基手性醇合成中的应用 |
CN111433357A (zh) * | 2018-02-12 | 2020-07-17 | 江南大学 | 醇脱氢酶突变体及其在双芳基手性醇合成中的应用 |
CN114341350A (zh) * | 2019-09-27 | 2022-04-12 | 艾希易股份公司 | 用于制备熊脱氧胆酸的方法 |
CN114606220A (zh) * | 2020-11-25 | 2022-06-10 | 湖南引航生物科技有限公司 | 固定化经修饰的7α-羟基甾体脱氢酶及其应用 |
CN114854707A (zh) * | 2022-06-14 | 2022-08-05 | 苏州百福安酶技术有限公司 | 一种7β-羟基甾体脱氢酶突变体 |
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Cited By (17)
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CN109722442A (zh) * | 2017-10-31 | 2019-05-07 | 中国科学院天津工业生物技术研究所 | 7β-羟基胆酸脱氢酶及其应用 |
CN109722442B (zh) * | 2017-10-31 | 2022-01-04 | 中国科学院天津工业生物技术研究所 | 7β-羟基胆酸脱氢酶及其应用 |
CN111094557B (zh) * | 2018-02-12 | 2022-11-11 | 江南大学 | 醇脱氢酶突变体及其在双芳基手性醇合成中的应用 |
CN111433357B (zh) * | 2018-02-12 | 2022-11-11 | 江南大学 | 醇脱氢酶突变体及其在双芳基手性醇合成中的应用 |
CN111094557A (zh) * | 2018-02-12 | 2020-05-01 | 江南大学 | 醇脱氢酶突变体及其在双芳基手性醇合成中的应用 |
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CN108546691A (zh) * | 2018-05-09 | 2018-09-18 | 华东理工大学 | 7β-羟基甾醇脱氢酶突变体及其在制备熊脱氧胆酸中的应用 |
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CN109182284B (zh) * | 2018-09-28 | 2020-12-04 | 湖南福来格生物技术有限公司 | 一种7β-羟基类固醇脱氢酶突变体、编码序列、重组表达载体、基因工程菌及应用 |
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CN114854707B (zh) * | 2022-06-14 | 2023-09-12 | 苏州百福安酶技术有限公司 | 一种7β-羟基甾体脱氢酶突变体 |
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