CN107099516B - 7β-羟基甾醇脱氢酶突变体及其在熊脱氧胆酸合成中的应用 - Google Patents
7β-羟基甾醇脱氢酶突变体及其在熊脱氧胆酸合成中的应用 Download PDFInfo
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- C—CHEMISTRY; METALLURGY
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
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- C—CHEMISTRY; METALLURGY
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CN108384765A (zh) * | 2018-02-12 | 2018-08-10 | 江南大学 | 醇脱氢酶突变体及其在双芳基手性醇合成中的应用 |
CN108504641A (zh) * | 2018-02-12 | 2018-09-07 | 江南大学 | 醇脱氢酶突变体及其在双芳基手性醇合成中的应用 |
CN108546691B (zh) * | 2018-05-09 | 2020-02-21 | 华东理工大学 | 7β-羟基甾醇脱氢酶突变体及其在制备熊脱氧胆酸中的应用 |
CN109182284B (zh) * | 2018-09-28 | 2020-12-04 | 湖南福来格生物技术有限公司 | 一种7β-羟基类固醇脱氢酶突变体、编码序列、重组表达载体、基因工程菌及应用 |
CN109486738B (zh) * | 2018-12-10 | 2020-08-04 | 江南大学 | 一种利用重组大肠杆菌全细胞转化生产宝丹酮的方法 |
IT201900017411A1 (it) * | 2019-09-27 | 2021-03-27 | Ice S P A | Processo di preparazione di acido ursodesossicolico |
CN114606220B (zh) * | 2020-11-25 | 2023-09-05 | 湖南引航生物科技有限公司 | 固定化经修饰的7α-羟基甾体脱氢酶及其应用 |
CN114854707B (zh) * | 2022-06-14 | 2023-09-12 | 苏州百福安酶技术有限公司 | 一种7β-羟基甾体脱氢酶突变体 |
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CN105441399A (zh) * | 2010-12-16 | 2016-03-30 | 细胞制药有限公司 | 新的7β-羟基类固醇脱氢酶突变体和制备熊去氧胆酸的方法 |
CN106086149A (zh) * | 2016-06-20 | 2016-11-09 | 苏州汉酶生物技术有限公司 | 一种化学‑酶法制备熊去氧胆酸的方法 |
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CN106086149A (zh) * | 2016-06-20 | 2016-11-09 | 苏州汉酶生物技术有限公司 | 一种化学‑酶法制备熊去氧胆酸的方法 |
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Engineering 7β-Hydroxysteroid Dehydrogenase for Enhanced Ursodeoxycholic Acid Production by Multiobjective Directed Evolution;Ming-Min Zheng等;《Journal of Agricultural and Food Chemistry》;20170124;第65卷(第6期);第1179右栏第3、5段、Scheme 1,第1180页右栏第1、4段,第1181页左栏第1-4段,第1183页右栏第2段以及补充材料表S5、图S5 * |
Two-step enzymatic synthesis of ursodeoxycholic acid with a new 7β-hydroxysteroid dehydrogenase from Ruminococcus torques;Ming-Min Zheng等;《Process Biochemistry》;20150117;第50卷(第4期);第600页左栏第1段 * |
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