CN105765060B - 羊膜间充质系细胞组合物的制造方法和冷冻保存方法、以及治疗剂 - Google Patents

羊膜间充质系细胞组合物的制造方法和冷冻保存方法、以及治疗剂 Download PDF

Info

Publication number
CN105765060B
CN105765060B CN201480046241.8A CN201480046241A CN105765060B CN 105765060 B CN105765060 B CN 105765060B CN 201480046241 A CN201480046241 A CN 201480046241A CN 105765060 B CN105765060 B CN 105765060B
Authority
CN
China
Prior art keywords
cells
mesenchymal
cell
collagenase
mesenchymal cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201480046241.8A
Other languages
English (en)
Chinese (zh)
Other versions
CN105765060A (zh
Inventor
山原研一
田口明彦
相马俊裕
大西俊介
小林明
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hokkaido University
Hyogo Medical University
Kaneka Corp
National Cerebral and Cardiovascular Center
Foundation for Biomedical Research and Innovation
Original Assignee
Hokkaido University
Hyogo Medical University
Kaneka Corp
National Cerebral and Cardiovascular Center
Foundation for Biomedical Research and Innovation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hokkaido University, Hyogo Medical University, Kaneka Corp, National Cerebral and Cardiovascular Center, Foundation for Biomedical Research and Innovation filed Critical Hokkaido University
Publication of CN105765060A publication Critical patent/CN105765060A/zh
Application granted granted Critical
Publication of CN105765060B publication Critical patent/CN105765060B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/50—Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3834—Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0668—Mesenchymal stem cells from other natural sources
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0669—Bone marrow stromal cells; Whole bone marrow
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
    • A61L2300/64—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/03—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from non-embryonic pluripotent stem cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Epidemiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Reproductive Health (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Virology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Rheumatology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Dermatology (AREA)
  • Transplantation (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Urology & Nephrology (AREA)
  • Botany (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
CN201480046241.8A 2013-08-19 2014-08-18 羊膜间充质系细胞组合物的制造方法和冷冻保存方法、以及治疗剂 Active CN105765060B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2013170008 2013-08-19
JP2013-170008 2013-08-19
JP2014130142A JP6512759B2 (ja) 2013-08-19 2014-06-25 羊膜間葉系細胞組成物の製造方法及び凍結保存方法、並びに治療剤
JP2014-130142 2014-06-25
PCT/JP2014/071546 WO2015025810A1 (ja) 2013-08-19 2014-08-18 羊膜間葉系細胞組成物の製造方法及び凍結保存方法、並びに治療剤

Publications (2)

Publication Number Publication Date
CN105765060A CN105765060A (zh) 2016-07-13
CN105765060B true CN105765060B (zh) 2020-04-28

Family

ID=52483585

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201480046241.8A Active CN105765060B (zh) 2013-08-19 2014-08-18 羊膜间充质系细胞组合物的制造方法和冷冻保存方法、以及治疗剂

Country Status (5)

Country Link
US (2) US10441611B2 (enExample)
EP (1) EP3037523B1 (enExample)
JP (1) JP6512759B2 (enExample)
CN (1) CN105765060B (enExample)
WO (1) WO2015025810A1 (enExample)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107849532A (zh) * 2015-05-14 2018-03-27 株式会社再生科技工程 冷冻间充质细胞的制备方法和移植用治疗材料的制备方法
CN109312302A (zh) * 2015-10-27 2019-02-05 株式会社钟化 包含间充质系干细胞的细胞群的制造方法、间充质系干细胞、细胞群、以及医药组合物
JP6727334B2 (ja) * 2016-02-01 2020-07-22 グリーン・クロス・ラブ・セル・コーポレイション 細胞の凍結保存のための培地組成物およびその使用
EP3564356A4 (en) * 2016-11-04 2020-05-06 The University Of Tokyo SOLUTION FOR THE CRYCON PRESERVATION OF ANIMAL CELLS OR ANIMAL TISSUE, CRYCON PRESERVED PRODUCT AND METHOD FOR THE CRYCON PRESERVATION
EP3543331A4 (en) 2016-11-15 2020-07-01 Kaneka Corporation CELL POPULATION CONTAINING MESENCHYMAL STEM CELLS DERIVED FROM F RATE APPENDICES, METHOD FOR PRODUCING THE SAME, AND MEDICINAL COMPOSITION
CN110494556B (zh) * 2017-04-03 2024-04-30 株式会社钟化 包含间充质系干细胞的细胞群、其制造方法、以及医药组合物
JP7140752B2 (ja) * 2017-04-03 2022-09-21 株式会社カネカ 胎児付属物由来接着細胞を含む細胞集団とその製造方法、及び医薬組成物
JP7152389B2 (ja) * 2017-04-03 2022-10-12 株式会社カネカ 間葉系幹細胞を含む細胞集団とその製造方法、間葉系幹細胞及び医薬組成物
CN106967675A (zh) * 2017-05-31 2017-07-21 东莞市保莱生物科技有限公司 一种从胎盘中分离提取造血干细胞的方法
WO2019059279A1 (ja) * 2017-09-20 2019-03-28 株式会社カネカ 細胞懸濁液を調製するためのデバイス、システム及び方法
JP7454379B2 (ja) * 2017-12-28 2024-03-22 株式会社カネカ 接着性幹細胞を含む細胞集団とその製造方法、及び医薬組成物
JP7526001B2 (ja) * 2017-12-28 2024-07-31 株式会社カネカ 接着性幹細胞を含む細胞集団とその製造方法、及び医薬組成物
CN108904533A (zh) * 2018-07-04 2018-11-30 卡替（上海）生物技术股份有限公司 牙髓间充质干细胞在制备肝硬化治疗药物中的用途
WO2020184350A1 (ja) 2019-03-08 2020-09-17 株式会社カネカ 多能性幹細胞の大量培養
WO2020251020A1 (ja) * 2019-06-14 2020-12-17 株式会社カネカ 間葉系細胞を含む細胞集団、それを含む医薬組成物、及び、その製造方法
US20230338428A1 (en) * 2020-09-08 2023-10-26 Amit Patel Allogenic umbilical cord stem cells for treating severe respiratory conditions
JP7672647B2 (ja) * 2021-02-05 2025-05-08 株式会社カネカ 間葉系幹細胞を含む細胞集団を含有する軟部組織再生用医薬組成物
WO2022203022A1 (ja) * 2021-03-26 2022-09-29 株式会社カネカ 羊膜由来間葉系幹細胞を含む細胞集団の製造方法
CN113456891B (zh) * 2021-06-16 2022-05-17 成都微沃科技有限公司 一种从细胞层中提取细胞外基质层的方法
CN114097768A (zh) * 2021-11-24 2022-03-01 李敬 一种骨髓干细胞用保护液及其制备方法和应用
WO2025150510A1 (ja) 2024-01-12 2025-07-17 株式会社カネカ 単一の間葉系幹細胞を単離する方法
CN120400046B (zh) * 2025-07-03 2025-10-17 济南万泉生物技术有限公司 一种羊膜基质细胞培养体系、细胞培养方法及应用

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL142914A0 (en) * 1998-11-09 2002-04-21 Consorzio Per La Gestione Del Serum free medium for chondrocyte-like cells
JP2000201672A (ja) 1999-01-11 2000-07-25 Asahi Medical Co Ltd 有核細胞の凍結保存用組成物
US8021876B2 (en) 2001-11-15 2011-09-20 Children's Medical Center Corporation Methods of isolation, expansion and differentiation of fetal stem cells from chorionic villus, amniotic fluid, and placenta and therapeutic uses thereof
JP3934539B2 (ja) 2001-12-12 2007-06-20 独立行政法人科学技術振興機構 胎盤等由来の成体又は生後組織の前駆細胞
US7176022B2 (en) 2002-12-20 2007-02-13 Cell Genesys, Inc. Directly injectable formulations which provide enhanced cryoprotection of cell products
CA2654716A1 (en) 2006-06-09 2007-12-21 Anthrogenesis Corporation Placental niche and use thereof to culture stem cells
CN103356710A (zh) 2007-02-12 2013-10-23 人类起源公司 利用胎盘干细胞治疗炎性疾病
JP2010530214A (ja) 2007-03-01 2010-09-09 クライオ−セル インターナショナル インコーポレイテッド 子宮内膜細胞／月経血細胞の獲得、単離および凍結保存
EP2014767A1 (en) 2007-06-15 2009-01-14 Centro di Ricerca E. Menni T cell immunomodulation by placenta cell preparations
WO2009058365A1 (en) 2007-10-31 2009-05-07 Cryo-Cell International, Inc. Methods for co-culturing cord blood derived cells with menstrual stem cells
NZ591292A (en) * 2008-08-20 2012-10-26 Anthrogenesis Corp Improved cell composition and methods of making the same
WO2010059828A1 (en) * 2008-11-19 2010-05-27 Anthrogenesis Corporation Amnion derived adherent cells
US20120149108A1 (en) * 2009-08-19 2012-06-14 Masashige Tanabe Cell preservation method
WO2011103462A1 (en) 2010-02-18 2011-08-25 Osiris Therapeutics, Inc. Methods of manufacture of immunocompatible amniotic membrane products
EP2731440A4 (en) * 2011-07-15 2015-04-29 Anthrogenesis Corp RADIATION LESSON TREATMENT USING ADHESENT CELLS DERIVED FROM AMNIOS

Also Published As

Publication number Publication date
US20160228474A1 (en) 2016-08-11
US11389486B2 (en) 2022-07-19
CN105765060A (zh) 2016-07-13
EP3037523A4 (en) 2017-01-25
JP2015061520A (ja) 2015-04-02
WO2015025810A1 (ja) 2015-02-26
US10441611B2 (en) 2019-10-15
US20190343892A1 (en) 2019-11-14
EP3037523B1 (en) 2020-08-12
JP6512759B2 (ja) 2019-05-15
EP3037523A1 (en) 2016-06-29

Similar Documents

Publication Publication Date Title
US11389486B2 (en) Method for producing amniotic mesenchymal stromal cell composition, method for cryopreserving the same, and therapeutic agent
JP2022060520A (ja) 間葉系幹細胞を含む細胞集団の製造方法、間葉系幹細胞、細胞集団、並びに医薬組成物
US10329533B2 (en) Regenerative cell and adipose-derived stem cell processing system and method
CN106465710B (zh) 脂肪组织冻存液及脂肪组织冻存方法
JP7530893B2 (ja) 間葉系細胞を含む細胞集団、それを含む医薬組成物、及び、その製造方法
CN103074298A (zh) 一种人脂肪间充质干细胞库及其构建方法
CN111088226A (zh) 一种胎盘间充质干细胞外泌体的制备和储存方法
CN101848718A (zh) 用于组织再生的细胞组合物
Mattei et al. Validated methods for isolation and qualification of mesenchymal stromal/stem cells from different sources
CN114134114B (zh) 从胎盘组织中扩增自然杀伤细胞的方法
US11760976B2 (en) Stem cells and decellularization of tissue matrix from cord tissue
JP6754459B2 (ja) 羊膜間葉系細胞組成物の製造方法及び凍結保存方法、並びに治療剤
CN112029713A (zh) 一种绒毛膜间充质干细胞分离培养扩增方法
WO2018186420A1 (ja) 間葉系幹細胞を含む細胞集団とその製造方法、間葉系幹細胞及び医薬組成物
JP7672647B2 (ja) 間葉系幹細胞を含む細胞集団を含有する軟部組織再生用医薬組成物
CN116144583B (zh) 一种获取人胎盘胚源干细胞的方法及应用
CN114231487B (zh) 胎盘来源的自然杀伤细胞的培养方法
US20240026299A1 (en) Stem cell and tissue cultures and their products
TWI454307B (zh) Method and application for preparing human plasma for cell culture
CN111979189A (zh) 一种羊膜间充质干细胞分离培养扩增方法
JP2025511281A (ja) 血清代替品及びその調製方法と用途

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
CB02 Change of applicant information
CB02 Change of applicant information

Address after: Osaka Japan

Applicant after: National Cerebral and Cardiovascular Center

Applicant after: Public welfare corporates, Kobe medical industry city promotion organization

Applicant after: Hyogo College of Medicine

Applicant after: National University Corporation Hokkaido University

Applicant after: Kaneka Corporation

Address before: Osaka Japan

Applicant before: National Cerebral and Cardiovascular Center

Applicant before: FOUNDATION FOR BIOMEDICAL RESEARCH AND INNOVATION

Applicant before: Hyogo College of Medicine

Applicant before: National University Corporation Hokkaido University

Applicant before: Kaneka Corporation

GR01 Patent grant
GR01 Patent grant