CN105765060B - 羊膜间充质系细胞组合物的制造方法和冷冻保存方法、以及治疗剂 - Google Patents

羊膜间充质系细胞组合物的制造方法和冷冻保存方法、以及治疗剂 Download PDF

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CN105765060B
CN105765060B CN201480046241.8A CN201480046241A CN105765060B CN 105765060 B CN105765060 B CN 105765060B CN 201480046241 A CN201480046241 A CN 201480046241A CN 105765060 B CN105765060 B CN 105765060B
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cells
cell
mesenchymal
collagenase
thermolysin
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CN105765060A (zh
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山原研一
田口明彦
相马俊裕
大西俊介
小林明
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Hokkaido University NUC
Hyogo College of Medicine
Kaneka Corp
National Cerebral and Cardiovascular Center
Foundation for Biomedical Research and Innovation at Kobe
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Hokkaido University NUC
Hyogo College of Medicine
Kaneka Corp
National Cerebral and Cardiovascular Center
Foundation for Biomedical Research and Innovation at Kobe
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CN201480046241.8A 2013-08-19 2014-08-18 羊膜间充质系细胞组合物的制造方法和冷冻保存方法、以及治疗剂 Active CN105765060B (zh)

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JP2013-170008 2013-08-19
JP2013170008 2013-08-19
JP2014130142A JP6512759B2 (ja) 2013-08-19 2014-06-25 羊膜間葉系細胞組成物の製造方法及び凍結保存方法、並びに治療剤
JP2014-130142 2014-06-25
PCT/JP2014/071546 WO2015025810A1 (ja) 2013-08-19 2014-08-18 羊膜間葉系細胞組成物の製造方法及び凍結保存方法、並びに治療剤

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Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3296392A4 (en) * 2015-05-14 2018-11-21 Advanced Cell Technology and Engineering Ltd. Method for producing frozen mesenchymal cells and method for producing implantable therapeutic member
WO2017073656A1 (ja) * 2015-10-27 2017-05-04 株式会社カネカ 間葉系幹細胞を含む細胞集団の製造方法、間葉系幹細胞、細胞集団、並びに医薬組成物
EP3412149A4 (en) * 2016-02-01 2019-09-18 Green Cross Lab Cell Corporation COMPOSITION OF A MEDIUM FOR CRYOCONIZING CELLS AND USES THEREOF
WO2018084228A1 (ja) * 2016-11-04 2018-05-11 国立大学法人東京大学 動物細胞又は動物組織の凍結保存用溶液、凍結物、及び凍結保存方法
WO2018092769A1 (ja) 2016-11-15 2018-05-24 株式会社カネカ 胎児付属物に由来する間葉系幹細胞を含む細胞集団とその製造方法、及び医薬組成物
EP3608399A4 (en) * 2017-04-03 2021-01-13 Kaneka Corporation CELL POPULATION CONTAINING ADHERENT CELLS DERIVED FROM THE FETAL APPENDIX AS WELL AS A METHOD FOR MANUFACTURING THE SAME, AND PHARMACEUTICAL COMPOSITION
JP7102397B2 (ja) 2017-04-03 2022-07-19 株式会社カネカ 間葉系幹細胞を含む細胞集団とその製造方法、及び医薬組成物
JP7152389B2 (ja) * 2017-04-03 2022-10-12 株式会社カネカ 間葉系幹細胞を含む細胞集団とその製造方法、間葉系幹細胞及び医薬組成物
CN106967675A (zh) * 2017-05-31 2017-07-21 东莞市保莱生物科技有限公司 一种从胎盘中分离提取造血干细胞的方法
WO2019059279A1 (ja) * 2017-09-20 2019-03-28 株式会社カネカ 細胞懸濁液を調製するためのデバイス、システム及び方法
JP7454379B2 (ja) * 2017-12-28 2024-03-22 株式会社カネカ 接着性幹細胞を含む細胞集団とその製造方法、及び医薬組成物
EP3733838A4 (en) * 2017-12-28 2021-11-03 Kaneka Corporation CELL POPULATION WITH ADHESIVE STEM CELLS, METHOD FOR MANUFACTURING SUCH CELL POPULATION AND PHARMACEUTICAL COMPOSITION
CN108904533A (zh) * 2018-07-04 2018-11-30 卡替（上海）生物技术股份有限公司 牙髓间充质干细胞在制备肝硬化治疗药物中的用途
JPWO2020184350A1 (enExample) 2019-03-08 2020-09-17
JP7530893B2 (ja) * 2019-06-14 2024-08-08 株式会社カネカ 間葉系細胞を含む細胞集団、それを含む医薬組成物、及び、その製造方法
MX2023002726A (es) * 2020-09-08 2023-05-18 Amit Patel Citoblastos alogenicos de cordón umbilical para el tratamiento de condiciones respiratorias graves.
JP7672647B2 (ja) * 2021-02-05 2025-05-08 株式会社カネカ 間葉系幹細胞を含む細胞集団を含有する軟部組織再生用医薬組成物
JPWO2022203022A1 (enExample) * 2021-03-26 2022-09-29
CN113456891B (zh) * 2021-06-16 2022-05-17 成都微沃科技有限公司 一种从细胞层中提取细胞外基质层的方法
CN114097768A (zh) * 2021-11-24 2022-03-01 李敬 一种骨髓干细胞用保护液及其制备方法和应用
CN120400046B (zh) * 2025-07-03 2025-10-17 济南万泉生物技术有限公司 一种羊膜基质细胞培养体系、细胞培养方法及应用

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU758073B2 (en) * 1998-11-09 2003-03-13 Consorzio Per La Gestione Del Centro Di Biotecnologie Avanzate Serum free medium for chondrocyte-like cells
JP2000201672A (ja) * 1999-01-11 2000-07-25 Asahi Medical Co Ltd 有核細胞の凍結保存用組成物
JP4330995B2 (ja) 2001-11-15 2009-09-16 チルドレンズ メディカル センター コーポレーション 絨毛膜絨毛、羊水、および胎盤からの胎児性幹細胞を単離、増殖、および分化させる方法、ならびにその治療的使用方法
JP3934539B2 (ja) 2001-12-12 2007-06-20 独立行政法人科学技術振興機構 胎盤等由来の成体又は生後組織の前駆細胞
US7176022B2 (en) 2002-12-20 2007-02-13 Cell Genesys, Inc. Directly injectable formulations which provide enhanced cryoprotection of cell products
AU2007258514A1 (en) 2006-06-09 2007-12-21 Anthrogenesis Corporation Placental niche and use thereof to culture stem cells
PL2120977T3 (pl) 2007-02-12 2013-12-31 Anthrogenesis Corp Leczenie chorób zapalnych z zastosowaniem łożyskowych komórek macierzystych
SG179432A1 (en) 2007-03-01 2012-04-27 Mercedes A Walton Procurement, isolation and cryopreservation of endometrial/menstrual cells
EP2014767A1 (en) 2007-06-15 2009-01-14 Centro di Ricerca E. Menni T cell immunomodulation by placenta cell preparations
TW200936148A (en) * 2007-10-31 2009-09-01 Cryo Cell Int Methods for co-culturing cord blood derived cells with menstrual stem cells
EP3172963B1 (en) * 2008-08-20 2019-02-06 Celularity, Inc. Improved cell composition and methods of making the same
RU2562154C2 (ru) 2008-11-19 2015-09-10 Антродженезис Корпорейшн Амниотические адгезивные клетки
EP2468850A4 (en) * 2009-08-19 2013-02-20 Takara Bio Inc CELL PRESERVATION PROCESS
EP2536827B1 (en) 2010-02-18 2018-01-10 Osiris Therapeutics, Inc. Methods of manufacture of therapeutic products comprising vitalized placental dispersions
WO2013012698A1 (en) * 2011-07-15 2013-01-24 Anthrogenesis Corporation Treatment of radiation injury using amnion derived adherent cells

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US10441611B2 (en) 2019-10-15
US20190343892A1 (en) 2019-11-14
US20160228474A1 (en) 2016-08-11
US11389486B2 (en) 2022-07-19
WO2015025810A1 (ja) 2015-02-26
EP3037523B1 (en) 2020-08-12
EP3037523A4 (en) 2017-01-25
JP2015061520A (ja) 2015-04-02
CN105765060A (zh) 2016-07-13
JP6512759B2 (ja) 2019-05-15

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