CN105026551B - 用于hUTC生长的营养富集培养基 - Google Patents

用于hUTC生长的营养富集培养基 Download PDF

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Publication number
CN105026551B
CN105026551B CN201380072759.4A CN201380072759A CN105026551B CN 105026551 B CN105026551 B CN 105026551B CN 201380072759 A CN201380072759 A CN 201380072759A CN 105026551 B CN105026551 B CN 105026551B
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cells
medium
cell
microcarriers
serum
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Expired - Fee Related
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Chinese (zh)
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CN105026551A (zh
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R.布哈蒂亚
L.S.K.康
S.S.奥兹图克
V.H.卡马拉祖
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DePuy Synthes Products Inc
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DePuy Synthes Products Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C12N2500/33—Amino acids other than alpha-amino carboxylic acids, e.g. beta-amino acids, taurine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Hematology (AREA)
  • Rheumatology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201380072759.4A 2012-12-14 2013-12-12 用于hUTC生长的营养富集培养基 Expired - Fee Related CN105026551B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010511518.7A CN111690602A (zh) 2012-12-14 2013-12-12 用于hUTC生长的营养富集培养基

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13/715532 2012-12-14
US13/715,532 US20140170748A1 (en) 2012-12-14 2012-12-14 Nutrient Enriched Media for hUTC Growth
PCT/US2013/074615 WO2014093598A1 (en) 2012-12-14 2013-12-12 NUTRIENT ENRICHED MEDIA FOR hUTC GROWTH

Related Child Applications (1)

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CN202010511518.7A Division CN111690602A (zh) 2012-12-14 2013-12-12 用于hUTC生长的营养富集培养基

Publications (2)

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CN105026551A CN105026551A (zh) 2015-11-04
CN105026551B true CN105026551B (zh) 2020-07-03

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CN201380072759.4A Expired - Fee Related CN105026551B (zh) 2012-12-14 2013-12-12 用于hUTC生长的营养富集培养基
CN202010511518.7A Pending CN111690602A (zh) 2012-12-14 2013-12-12 用于hUTC生长的营养富集培养基

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US (1) US20140170748A1 (enExample)
EP (2) EP2931875B1 (enExample)
JP (2) JP6404227B2 (enExample)
KR (2) KR102151588B1 (enExample)
CN (2) CN105026551B (enExample)
AR (2) AR093985A1 (enExample)
AU (2) AU2013359265B2 (enExample)
BR (1) BR112015013685A8 (enExample)
CA (1) CA2895079C (enExample)
ES (1) ES2667573T3 (enExample)
MX (2) MX364731B (enExample)
PH (1) PH12015501310B1 (enExample)
PL (1) PL2931875T3 (enExample)
RU (2) RU2684306C2 (enExample)
SG (2) SG11201504547SA (enExample)
TW (1) TWI620818B (enExample)
WO (1) WO2014093598A1 (enExample)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10791730B2 (en) * 2016-01-14 2020-10-06 DePuy Synthes Products, Inc. Composition and methods for cryopreservation of hUTC
US10894947B1 (en) * 2016-04-29 2021-01-19 Hope Biosciences, Llc Method for generating protein rich conditioned medium
US11111480B2 (en) 2016-04-29 2021-09-07 Hope Biosctences, Llc Culture media for multipotent stem cells
CN106434532A (zh) * 2016-12-22 2017-02-22 叶宗耀 一种培养肝细胞的培养基及其制备方法
CN106957815B (zh) * 2017-03-16 2021-05-07 杨涛 一种用于人类多潜能干细胞的无血清培养基的配方
JP7181534B2 (ja) * 2017-03-28 2022-12-01 味の素株式会社 未分化維持培地添加剤
CN107119017B (zh) * 2017-05-25 2020-05-26 山东省医药生物技术研究中心 一种骨肉瘤细胞的无血清培养基及其制备方法
KR20210068488A (ko) * 2018-09-28 2021-06-09 아지노모토 가부시키가이샤 동물 세포의 배양용 첨가물, 배양용 배지 및 배양 방법
EP3947642A4 (en) * 2019-04-05 2022-12-14 The University of Hong Kong CULTURE MEDIUM FOR MAMMALIAN PLURIPOTENTE STEM CELLS, COMPOSITION AND METHODS
WO2020219812A1 (en) 2019-04-26 2020-10-29 Allogene Therapeutics, Inc. Methods of manufacturing allogeneic car t cells
EP3983481A1 (en) * 2019-06-13 2022-04-20 Corning Incorporated Dissolvable microcarriers for culturing cells and related methods
CN114058573B (zh) * 2020-08-03 2024-04-05 上海原天生物科技有限公司 一种含有生物素的培养基
CN112608892B (zh) * 2020-12-25 2023-11-14 深圳博雅感知药业有限公司 血小板裂解物用于无血清分离和传代培养脐带间充质干细胞的方法
KR20220104321A (ko) 2021-01-18 2022-07-26 주식회사 엑소코바이오 엑소좀 및/또는 세포외 소포체의 생성 촉진 내지는 생산성 향상 방법
US11735303B2 (en) 2021-06-22 2023-08-22 David Haase Apparatus and method for determining a composition of a replacement therapy treatment
WO2024253069A1 (ja) * 2023-06-06 2024-12-12 東ソー株式会社 温度応答性マイクロキャリア
KR20250140345A (ko) * 2024-03-18 2025-09-25 주식회사 심플플래닛 세포 증식 활성이 우수한 식용 배지 조성물

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060094113A1 (en) * 2004-10-29 2006-05-04 David Epstein Chemically defined media compositions
CN101182490A (zh) * 2007-11-27 2008-05-21 天津百若克医药生物技术有限责任公司 一种用于Vero细胞的培养基及其培养方法
CN101611139A (zh) * 2006-11-13 2009-12-23 伊西康公司 利用微载体的产后来源的细胞的体外扩增
US20120014921A1 (en) * 2003-06-27 2012-01-19 Advanced Technologies And Regenerative Medicine, Llc Treatment of stroke and other acute neural degenerative disorders via intranasal administration of umbilical cord-derived cells

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA776251B (en) * 1976-11-11 1978-07-26 Massachusetts Inst Technology Improved cell culture microcarriers
WO1991010726A1 (en) * 1990-01-22 1991-07-25 The United States Of America, Represented By The Secretary, United States Department Of Commerce Co-independent growth medium for maintenance and propagation of cells
JP2993614B2 (ja) * 1990-11-20 1999-12-20 有限会社野々川商事 新規化粧料、グルコサミノグリカン生成促進剤および美白剤
US7556958B1 (en) 1997-04-08 2009-07-07 The Rockefeller University Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof
CA2438148A1 (en) * 2001-02-15 2002-08-29 Centocor, Inc. Chemically defined medium for cultured mammalian cells
US9969980B2 (en) 2001-09-21 2018-05-15 Garnet Biotherapeutics Cell populations which co-express CD49c and CD90
WO2003057865A1 (es) * 2002-01-09 2003-07-17 Derbiotek, S.L. Composicion y procedimiento para crear, regenerar y reparar tejidos mediante implante biologico portador de celulas y enriquecido con concentrado de plaquetas mas suplementos.
CA2530421C (en) 2003-06-27 2015-04-21 Ethicon, Incorporated Repair and regeneration of ocular tissue using postpartum-derived cells
US20050005863A1 (en) 2003-07-10 2005-01-13 Snider Tracy Aurora Christine Distillers solubles as the primary constituent in protein blocks for livestock
EP1682075A4 (en) * 2003-11-07 2009-12-09 Univ Florida BREEDING AND DIFFERENTIATION OF NEURAL CREATOR CELLS
JP5340599B2 (ja) * 2004-12-23 2013-11-13 エシコン・インコーポレイテッド 臍帯組織由来産褥細胞ならびにその製造方法および使用方法
JP5179376B2 (ja) 2005-12-19 2013-04-10 エシコン・インコーポレイテッド ローラーボトルでの分娩後取り出し細胞の体外増殖
AU2007270069B2 (en) * 2006-07-06 2013-05-16 Es Cell International Pte Ltd Method for stem cell culture and cells derived therefrom
US20100143289A1 (en) * 2006-12-19 2010-06-10 Michael Cohen Umbilical cord stem cell secreted product derived topical compositions and methods of use thereof
EP2028268A1 (en) * 2007-08-20 2009-02-25 Université Libre De Bruxelles Generation of neuronal cells from pluripotent stem cells
US10179900B2 (en) * 2008-12-19 2019-01-15 DePuy Synthes Products, Inc. Conditioned media and methods of making a conditioned media
JP2013500717A (ja) * 2009-07-28 2013-01-10 コーニング インコーポレイテッド 細胞を培養するための合成マイクロキャリア

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120014921A1 (en) * 2003-06-27 2012-01-19 Advanced Technologies And Regenerative Medicine, Llc Treatment of stroke and other acute neural degenerative disorders via intranasal administration of umbilical cord-derived cells
US20060094113A1 (en) * 2004-10-29 2006-05-04 David Epstein Chemically defined media compositions
CN101611139A (zh) * 2006-11-13 2009-12-23 伊西康公司 利用微载体的产后来源的细胞的体外扩增
CN101182490A (zh) * 2007-11-27 2008-05-21 天津百若克医药生物技术有限责任公司 一种用于Vero细胞的培养基及其培养方法

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AU2013359265B2 (en) 2019-01-31
AR118040A2 (es) 2021-09-15
HK1215050A1 (en) 2016-08-12
CA2895079A1 (en) 2014-06-19
JP2018183195A (ja) 2018-11-22
ES2667573T3 (es) 2018-05-11
US20140170748A1 (en) 2014-06-19
CN111690602A (zh) 2020-09-22
BR112015013685A8 (pt) 2019-11-05
EP2931875B1 (en) 2018-03-21
MX364731B (es) 2019-05-06
TW201439321A (zh) 2014-10-16
SG10201801924VA (en) 2018-05-30
KR101948237B1 (ko) 2019-02-14
SG11201504547SA (en) 2015-07-30
EP3382007A1 (en) 2018-10-03
JP2016501026A (ja) 2016-01-18
PH12015501310A1 (en) 2015-09-02
PL2931875T3 (pl) 2018-08-31
TWI620818B (zh) 2018-04-11
AU2013359265A1 (en) 2015-07-16
PH12015501310B1 (en) 2015-09-02
RU2018112452A (ru) 2019-03-01
JP6404227B2 (ja) 2018-10-10
WO2014093598A1 (en) 2014-06-19
MX2018013469A (es) 2020-11-12
RU2684306C2 (ru) 2019-04-05
KR20190016612A (ko) 2019-02-18
RU2653449C2 (ru) 2018-05-08
AU2019202191B2 (en) 2021-01-21
RU2015128276A (ru) 2017-01-23
BR112015013685A2 (pt) 2017-07-11
MX2015007594A (es) 2016-03-15
EP2931875A1 (en) 2015-10-21
CN105026551A (zh) 2015-11-04
JP6595061B2 (ja) 2019-10-23
KR102151588B1 (ko) 2020-09-04
RU2018112452A3 (enExample) 2019-03-01
KR20150094760A (ko) 2015-08-19
AR093985A1 (es) 2015-07-01
CA2895079C (en) 2021-06-29
AU2019202191A1 (en) 2019-04-18

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