CN104956984B - A kind of greenhouse fig cutting propagation method for culturing seedlings - Google Patents
A kind of greenhouse fig cutting propagation method for culturing seedlings Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The invention discloses a kind of greenhouse fig cutting propagation method for culturing seedlings, and it is cuttings to take length 15cm figs to give birth to semi-lignified branch then, lower end sterilization, utilizes 800mg/L ABT2 promoter immersion treatments;In Optimization-type heliogreenhouse, bottom and extraneous 10~15 DEG C of the temperature difference are realized in slotting machine inner bottom part laying heating wire;The cutting medium that the cuttings handled well insertion is mixed by turf, decomposed cow dung, vermiculite, perlite and bone meal;After cuttage, sprayed and managed using full exposure, make water content 20~25% in greenhouse;45d, 60d and 75d after cuttage, respectively spray foliar fertilizer 1 time, carry out Routine Management.The fig seedling percent more than 95% that the inventive method is cultivated, not only shorten cuttings callus and adventitious root apparition, it is especially the most favourable to cuttage seeding callus, moreover it is possible to improve its take root number, root long and rooting rate etc., strengthen seedling resistance, promote fig nursery stock robust growth.
Description
Technical field
The invention belongs to field of fruit tree cultivation, is related to a kind of greenhouse fig cutting propagation method for culturing seedlings.
Background technology
Fig (Ficus carica L.) is the perennial fruit tree of Moraceae Ficus, originates in mediterranean country, is in the world
Most ancient one of to cultivate apple trees, on the ground such as Xinjiang of China, Shandong, cultivation history is long, and hidden headdress flower is formed because expanding its holder
Sequence, therefore claim " fig ".Rich in the mankind such as several amino acids, vitamin and polysaccharide balance in the nutrition organs such as its fruit, stem, leaf
The important component of meals, have edible, medicinal and view and admire etc. and to be worth, exploitation prospect is wide.As traditional fruit tree
Seedling-wood breeding method, cutting propagation are the important channels of fig seedling breeding, especially with the artificial synthesized of auxin and
The application of auto spraying, correlation technique measure have been widely used in fig greenhouse production there occurs development with rapid changepl. never-ending changes and improvements
Production, material base is established for fruit quality improving and breed breeding.The height of fig cuttage shoot survival percent, quality it is excellent
Bad and adaptability and cuttage technique close relation, and existing cuttage technique is far from the need for meeting seedling large-scale production
Ask, therefore explore a kind of high-quality and efficient cuttage raise seedling technique to turn into the big problem urgently tackled key problems in current fig industry,
Solid technical guarantee will be established for breeding for improved seeds.
The content of the invention
The purpose of the present invention is the above-mentioned deficiency for prior art, there is provided a kind of greenhouse fig cutting propagation nursery side
Method.
The purpose of the present invention can be achieved through the following technical solutions:
A kind of greenhouse fig cutting propagation method for culturing seedlings, is comprised the steps of:
(1) take length be 12~15cm robust growths, tissue enrich then give birth to semi-lignified fig branch be slotting
Fringe, each 2~3 leaves of cuttings band, upper end are whittled into horse ear shape inclined-plane away from truncate at terminal bud 2cm at the axillary bud of lower end;
(2) to cuttings carry out sterilizing processing, reuse 600~800mg/L ABT2 promoters immersion treatment 1~
3min;
(3) in Optimization-type heliogreenhouse, cutting medium bottom in slotting machine paving heating wire, realize substrate base with it is outer
10~15 DEG C of boundary's temperature difference, the cuttings that previous step is handled well insert cutting medium, and 2~3cm is revealed in cuttings upper end;Wherein described skewer
Insert matrix be turf, decomposed cow dung, vermiculite, perlite and bone meal mix, described turf, decomposed cow dung, vermiculite with
The volume ratio of perlite is 6~8:4~6:2~4:1~3, described bone meal accounts for the 5~8% of cutting medium cumulative volume;
(4) after cuttage, sprayed and managed using full exposure, make water content 20~25% in greenhouse;After cuttage 45d, 60d and
75d, respectively sprays foliar fertilizer 1 time, carries out conventional field management;Cultivate 3~4 months and obtain cuttage seeding.
Method cuttings of the present invention carries out sterilizing processing:After cuttings is cut its lower end 800~
10~15min of immersion treatment in 1000mg/L carbendazim thimerosals, its lower end is in 800mg/L further preferably after cuttings is cut
12min is soaked in carbendazim thimerosal.
Before the preferred cutting medium cuttage of method of the present invention using 4000mg/L network ammonia copper, 160mg/L hymexazols and
The sterilization of 1200mg/L carbendazim is mixed thoroughly stand-by;1d slotting machines are poured permeable before cuttage, and slotting machine is disappeared with 0.1% liquor potassic permanganate
Poison.
The preferred cutting length of method of the present invention is 15cm, is used for using 800mg/L ABT2 promoters processing 2min
Cuttage.
In method of the present invention, turf in described cutting medium, decomposed cow dung, the volume ratio of vermiculite and perlite
Preferably 6:4:2:1, described bone meal accounts for the 6% of cutting medium cumulative volume.
Method of the present invention preferably using full exposure spraying management, makes water content 25% in greenhouse.
The distance between rows and hills of cuttage is preferably 20 × 15cm in method and step (3) of the present invention.
Foliar fertilizer described in method and step (4) of the present invention is that proportioning is that N, P, K are followed successively by 300mg/L, 25mg/
L and 50mg/L nutrient solution.Described cuttage seeding is aerial part high 75~85cm, 1.0~1.5cm of base diameter cuttage
Seedling.
Optimization-type heliogreenhouse of the present invention, it include rear wall, gable, rear roofing, front roof, front roof bow member,
Between greenhouse front foot and buffering;Described rear wall include it is fixed after wall and wall support structure, after described fixation wall by it is interior outward
Including recuperation layer, thermal insulation layer and heat-insulation layer;Described recuperation layer is piled up by the building-block filled with clayed soil to be formed, described
Thermal insulation layer be styrofoam, described heat-insulation layer is piled up by the building-block filled with stalk block to be formed;In described wall branch
Support structure is provided with wall after dismounting, and wall freely can be installed and dismantled after dismounting, and wall is piled up by stalk block and formed after described dismounting;
The inner side of wall is covered with fly net after described dismounting, and the outer side covers of wall have plastic sheeting after dismounting;Described wall support
Structure includes inner column, outer column, crossbeam, longeron and lacing wire, and the inner column arranges one per 4m along rear wall of greenhouse length interval
Place, outer column dislocation are arranged among two adjacent inner columns;Described front roof bow member is coating steel pipe fabricated construction,
Front roof bow member is hinged to assembled bow member including top boom, lower boom, connector, top boom and lower boom by connector,
The both ends of top boom and lower boom, which are respectively articulated with, is fixed on greenhouse front foot and wall top end after dismounting;In described front roof bow member
Top and sidepiece are respectively equipped with top portion ventilation window and sidepiece vent window, and described top portion ventilation window and sidepiece vent window are provided with preventing
The trend of worm net, top portion ventilation window and sidepiece vent window is horizontally-parallel with greenhouse, and width is 80~100cm;Described
Compressed on front roof bow member in addition to top sky air regulator and sidepiece vent window covered with canopy film, and with film-pressing line;Described rear roofing
By being rolled up from the interior single-faced color steel plate set gradually outward, expansion polyphenyl plate, cement, flyash haydites of book structure screed-coat, SBS pitches
Material waterproof layer, cement mortar protective course are formed;Between described buffering positioned at heliogreenhouse fixation after wall west side.
Preferably, the outer side covers of wall have the pull-type plastic sheeting of volume after described dismounting.
Preferably, it is equipped with the pull-type plastic sheeting of volume on top portion ventilation window and sidepiece vent window, leads to top during the rainy day
The pull-type plastic sheeting drop-down covering vent window of volume on air regulator and sidepiece vent window carries out waterproof.
Described canopy film is anti-aging polyethylene or polyvinyl chloride longevity fogging-resistance film.
Wall not load-bearing after the dismounting of described Optimization-type heliogreenhouse, but by inner column, outer column, lacing wire, crossbeam and
Longeron collectively constitutes a box supporting construction, and the structural stability can is stronger, and the load that Sunlight greenhouse frame is born
Directly pass in ground;
Processing of dripping is done on described rear wall wall top, overboard drenches Hou Qiang to reduce the rainy day.It is of the present invention excellent
After change type heliogreenhouse front roof bow member and rear roofing construction, carried out in rear roofing and Hou Qiang junctions and rear wall top portion
Waterproof and isothermal holding.
Beneficial effects of the present invention:
The present invention optimizes to traditional cuttage technique, high-quality by taking various technical measures to establish in production practices
Efficient greenhouse fig Technique on Cuttage Propagation system, can concentrate that solution fig cuttage shoot survival percent is low, and seedling is slow, grow not
The problems such as stable and shoot mass character difference, so promote produce in improve constantly the economic benefit of seeling industry.Greenhouse skewer
Be inserted on China's fig seedling year-round supply and played great function, not only alleviate production season and consumption equilibrium it
Between contradiction, also produce high-grade seedling using the technology, enrich nursery stock kind, famous-particular-excellent nursery stock, seasonal nursery stock, substantially meet
Vast production cultivation enterprise and the demand of orchard worker, enhance occupation rate and competitiveness of the fig seedling in fruits and vegetables market.In addition,
This technology can not only according to cultivation purpose difference be divided into facilitate cuttage, take shelter from rain cuttage and delay cuttage, and then flexibly grasp without flower
Fruit seedling harvest time, and increase it and fig seedling is further promoted to illumination, the demand of temperature, being precisely controlled for liquid manure
Fast-growth and maturation.
(1) present invention by greenhouse carry out fig cutting propagation seedling growth test, to greenhouse selection, Screening matrix,
Scion processing and cottage method are optimized, wherein, compared to plain edition heliogreenhouse, lew, vinyl house and glass temperature
The establishment types such as room, the fig cuttings callus apparition and adventitious root apparition in Optimization-type heliogreenhouse are shorter, skewer
Insert callus rate and rooting rate is high, it is very big that adventitious root effect is produced to cuttage.In addition, the heat storing and heat preserving effect of Optimization-type heliogreenhouse
Prominent, winter reduces heat and scattered and disappeared, and other season ventilation effects are uniform, are also equipped with insect prevention, waterproof, good appearance and operating space
The features such as big.Therefore, Optimization-type heliogreenhouse is the suitable establishment type of fig cutting propagation, should give large-scale demonstration and push away
Extensively.
(2) turf:Decomposed cow dung:Vermiculite:Perlite volume ratio is 6:4:2:1st, bone meal content is at 6% composite interstitial substance
The fig cuttings callus apparition of reason and adventitious root apparition are shorter, be cuttage tissue callus and indefinite root development most
Good environment, adventitious root effect maximum is produced to cuttage, the influence to epicormic branch cuttage callus rate and adventitious root is with facilitation.
(3) according to indexs such as callus apparition, adventitious root apparition, root long, radical and Rooting indexes, with reference to reality
Production, the better economic benefit for selecting 15cm cuttings to obtain;Cuttage root-taking number, rooting rate and the Rooting index that ABT2 is handled are equal
Height, the adventitious root apparition is early, and adventitious root is big;In actual production, cuttage seeding quality of rooting when 800mg/L is handled is high.To sum up institute
State, it is Optimal action that 15cm cuttings, which is aided with 800mg/L ABT2 promoters, the processing can remarkably promote cuttage seeding callus and
Indefinite root growth, it can be promoted in greenhouse fig cutting propagation and prevalent variety cultivation.
(4) experiment shows, the temperature difference combines the fig cuttage seeding callus for controlling wet-treating and the adventitious root apparition is short,
Big to adventitious root growth, cuttage seeding rooting rate is high, and caused adventitious root is more, indefinite root long, and quality of rooting is high.Therefore,
Single control is wet or temperature difference processing is unfavorable for growth of cuttage seedlings development, and it is numerous that temperature difference combination control wet process is applied to fig cuttage
Grow, promotional value is high.
It can be assisted between the factors of influence such as greenhouse, matrix formulations, cuttings processing and cottage method that the present invention optimizes
With working, more single optimization processing not only shortens fig cuttings callus and adventitious root apparition, especially to cuttage seeding
Callus is the most favourable, moreover it is possible to and cuttage seedling rooting number, root long, rooting rate and Rooting index etc. are improved, strengthens seedling resistance,
And then promote nursery stock robust growth.The fig cuttage shoot survival percent more than 95% that the present invention cultivates, can go out garden then, can be real
Now cuttage then, the purpose got bumper crops when year's harvest garden, when annual bearing and then.
Brief description of the drawings
Fig. 1 is the Optimization-type sunlight greenhouse structure schematic diagram of the specific embodiment of the invention.
Fig. 2 is the Optimization-type heliogreenhouse plan of the specific embodiment of the invention.
Fig. 3 is the Optimization-type heliogreenhouse I-I profiles of the specific embodiment of the invention.
Fig. 4 be the specific embodiment of the invention Optimization-type heliogreenhouse after wall inside structure figure.
Fig. 5 be the specific embodiment of the invention Optimization-type heliogreenhouse after wall outboard structure figure.
The transverse, top-down-view of wall after the Optimization-type heliogreenhouse of Fig. 6 specific embodiment of the invention is fixed.
Fig. 7 be the specific embodiment of the invention Optimization-type heliogreenhouse after wall longitudinal sectional drawing.
Wherein, wall after 1- is fixed, wall after 2- dismounting, 3- wall support structures, 4- gables, roofing after 5-, 6- front roofs, 7-
Front roof bow member, the front foot of 8- greenhouses, 9- recuperation layers, 10- thermal insulation layers, 11- heat-insulation layers, 12- inner columns, 13- outer columns, 14- are horizontal
Beam, 15- longerons, 16- lacing wires, 17- fly nets, 18- plastic sheetings, 19- top booms, 20- lower booms, 21- connectors, 21- connect
Fitting.
Embodiment
1 experimental field overview
This experiment is in facilities horticulture research institute of Xiuqian City (hereinafter referred to as " our hospital ") scientific base fig Germplasm Resources
Carry out, 15~22 DEG C of Growing season greenhouse temperature, humidity 70%~90%, sunshine is sufficient, and the content of organic matter is high in test material, partially
Acid.
2 test materials
Cuttings is kind fig robust growths such as ' the Japanese purple fruits ' of our hospital's introduction and acclimatization, tissue enriches raw then half
Lignifying branch, each 2~3 leaves of cuttings band, upper end are whittled into horse ear shape inclined-plane away from truncate at terminal bud 2cm at the axillary bud of lower end, insert
Fringe is cut soaks 12min by its lower end in 800mg/L carbendazim thimerosals afterwards.Matrix using 4000mg/L network ammonia copper,
160mg/L hymexazols and the sterilization of 1200mg/L carbendazim are mixed thoroughly, cuttings cuttage after processing.
3 experimental designs
Using random blocking experimental design completely, carry out fig cuttage and seedling culture in the facilities such as Optimization-type heliogreenhouse
Contrast test, select and optimize suitable greenhouse.15cm cuttings is aided with 800mg/L ABT2 root-growing agents, using different cuttage bases
Matter combines, and screens optimal seedling medium.On this basis, using orthogonal test analysis difference cutting length, hormone and concentration etc.
Treatment measures combine, and determine the treatment measures of cuttings.Carry out fig Cutting test using matrix temperature differential method etc., analyze not Tongfang
The index such as influence of the method to seedling root growth, statistics cuttage seedling rooting number, root length, rooting rate and survival rate, synthesis are commented
Valency fig cuttage rooting quality, preferably a kind of cottage method.It is innovative to key technology during cuttage integrated, establish greenhouse
Fig cuttage raise seedling technique system.
Influence of the 3.1 different greenhouses to cutting experiment
It is big in plain edition heliogreenhouse, Optimization-type heliogreenhouse, lew, plastics respectively using fig branch as examination material
Carry out cutting propagation nursery contrast test in the facility such as canopy and glasshouse, be shown in Table 1.30 cuttings, 3 weights are taken in each greenhouse
It is multiple.The random sampling after cuttage 1,8,15 and 22d, observes situation of taking root, and each item of testing takes 3 cuttings.
The different cuttage greenhouses of table 1
Optimization-type heliogreenhouse of the present invention is (open in utility model patent Winter-summer dual purpose reassembling type heliogreenhouse
Number 202857414U) and a kind of Novel solar greenhouse backwall structure (publication number 203378345U) technical scheme on the basis of transform and
Into.As shown in Figures 1 to 7, Optimization-type heliogreenhouse of the present invention, it includes wall 1 after fixing, wall 2, wall after dismounting
Between supporting construction 3, gable 4, rear roofing 5, front roof 6, front roof bow member 7, greenhouse front foot 8 and buffering;After described dismounting
Wall 2 is by setting the wall support structure 3 at the top of wall 1 after fixation to fix, wall after wall 1, wall support structure 3 and dismounting after fixing
Wall after 2 compositions;Wall 1 includes recuperation layer 9, thermal insulation layer 10 and heat-insulation layer 11, described recuperation layer 9 outward by interior after described fixation
Separated with heat-insulation layer 11 by thermal insulation layer 10;Described recuperation layer 9 is by filled with thermal conductivity factor, specific heat capacity and larger viscous of density
The building-block of property soil, which is piled up, to be formed, and the thermal insulation layer 10 is styrofoam, and described heat-insulation layer 11 is by filled with stalk block
Building-block, which is piled up, to be formed;Described wall support structure 3 includes inner column 12, outer column 13, crossbeam 14, longeron 15 and lacing wire
16, the inner column 12 along rear wall of greenhouse length interval per 4m at arrangement one, outer column 13 misplace be arranged in two it is adjacent interior
Among column 12;Wall 2 is piled up by stalk block and formed after described dismounting, freely can install and dismantle, and heat is reduced after winter installation
Amount is scattered and disappeared, and other seasons remove increase draught area;The inner side of wall 2 is easy to summer to prevent covered with fly net 17 after described dismounting
Worm, the outer side covers of wall 2 have the pull-type plastic sheeting 18 of volume to drench stalk block from rainwater after dismounting;Described front roof bow member 7
For coating steel pipe fabricated construction, front roof bow member 7 includes top boom 19, lower boom 20, connector 21, top boom 19 and lower edge
Bar 20 is hinged to assembled bow member by connector 21, the both ends of top boom 19 and lower boom 20 are respectively articulated be fixed on greenhouse before
The top of wall 2 after pin 8 and dismounting;Top portion ventilation window is respectively equipped with the top of described front roof bow member 7 and sidepiece and sidepiece leads to
Air regulator, described top portion ventilation window and sidepiece vent window are provided with fly net, and the trend of top portion ventilation window and sidepiece vent window is equal
Horizontally-parallel with greenhouse, width is 80~100cm, and sidepiece vent window can form cross-ventilation with top portion ventilation window, promote room
Interior air circulation, excluded rapidly by top portion ventilation window beneficial to hot-air, the both uniform temperature at indoor each position, and effectively dropped
Low indoor temperature, is equipped with the pull-type plastic sheeting of volume on top portion ventilation window and sidepiece vent window, by top portion ventilation during the rainy day
The pull-type plastic sheeting drop-down covering vent window of volume on window and sidepiece vent window carries out waterproof;On described front roof bow member 7
Compressed in addition to top sky air regulator and sidepiece vent window covered with canopy film, and with film-pressing line;Described rear roofing by from the bottom up according to
Asbestos board, expansion polyphenyl plate, cement, flyash haydites of book structure screed-coat, asphalt felt waterproof layer, the cement sand bed of secondary setting
Form;Between described buffering after the fixation of heliogreenhouse wall 1 west side, its length direction can freely extend, Interior Space
Between can as dismounting after wall storeroom and stack farm implements, or greenhouse management worker live etc..
Processing of dripping is done on described rear wall wall top, overboard drenches Hou Qiang to reduce the rainy day.Described Optimization-type day
The not load-bearing of wall 2 after the dismounting in light greenhouse, but collectively constituted by inner column 12, outer column 13, lacing wire 16, crossbeam 14 and longeron 15
One box supporting construction, the structural stability can is stronger, and the load that Sunlight greenhouse frame is born directly is passed in ground.
Described Optimization-type heliogreenhouse front foot and wall basis use strip footing;Described canopy film for anti-aging polyethylene or
Polyvinyl chloride longevity fogging-resistance film, it is covered on assembled bow member and is compressed with film-pressing line.
In a particular embodiment, Optimization-type heliogreenhouse net span 9.8m, greenhouse length 50.2m, the high 3.95m of greenhouse ridge,
The high 2.8m of rear wall of greenhouse.Wherein, 1 high 1.6m of wall, buried depth 0.5m, thick 0.65m after fixing, include 40cm thickness recuperation layers outward by interior
9th, 3cm thickness thermal insulation layer 10 and 20cm thick insulation layers 11, each 1cm of cement mortar troweling is used inside and outside wall;Wall 1 builds after fixation
It is single row of holes concrete small size hollow building block to build material, and size is 390 × 190 × 190mm, and internal layer building-block fills cohesive soil
Earth;Stalk block is filled in outer hollow building block;Thermal insulation layer 6 is styrofoam;2 high 1.2m of wall after dismounting.25 ° of front roof angle, the elevation angle
Depending on the actual sunshine characteristic of China Huanghuai Area area.Wall support structure 3 is made up of zinc-plated component, inner column 12, outer
Column 13, lacing wire 16, the size of crossbeam 14 and longeron 15 are respectively 70 × 50 × 3.0mm zinc-plated square steels, 50 × 50 × 2.0mm platings
Zinc square steel, 40 × 40 × 2.0mm Zinc-coated angle steels, 50 × 50 × 2.0mm zinc-plated square steels and 50 × 50 × 2.0mm zinc-plated square steels.Institute
The front roof bow member 7 stated is coating steel pipe, wherein top boom 19, the size of top boom 20 be respectively Φ 32 × 2.5, Φ 25 ×
2.0.The high 3.3m of top portion ventilation window on described front roof bow member 7, its trend, width 100cm parallel with the length in greenhouse;
The high 1.6m of sidepiece vent window positioned at the southern side of front roof bow member 7, parallel with the length in greenhouse, the width 60cm of its trend.Institute
Rear roofing 5, the rear roofing 5 stated carry out waterproof and isothermal holding with Hou Qiang junctions and rear wall top portion, are specially:From interior past
Set gradually 5cm thickness single-faced colors steel plate, 10cm thickness expansion polyphenyl plate, 2cm thickness 1 outside:0.2:3.5 cement, flyash haydites of book structure are looked for
Flat bed, 2cm thickness SBS asphalt felts waterproof layer and 2cm thickness 1:3 cement mortar protective courses.
The heat storing and heat preserving effect of Optimization-type heliogreenhouse protrudes, and winter reduces heat and scattered and disappeared, and other season ventilation effects are equal
It is even, the features such as being also equipped with insect prevention, waterproof, good appearance and big operating space.
Rear wall and the vent window setting of plain edition heliogreenhouse of the present invention are different from Optimization-type heliogreenhouse, its
His structure is identical.Rear wall high 2.8m, the thick 0.6mm of plain edition heliogreenhouse, by building-block fill heat transfer coefficient, specific heat capacity and
The less perlite of density is formed, and is provided with vent window, vent window terrain clearance 1.4m every 3m on rear wall, vent window size is
30cm × 40cm, along wall inside and outside it is double-deck.
Influence of 3.2 different substrates to cutting experiment
800mg/L ABT2 promoters are aided with 15cm fig cuttings, substrate combination is shown in Table 2, contained in the formula
6% (v/v) bone meal.Every group of experiment takes 30 cuttings, 3 repetitions.The 1st, 8,15 and 22d random samplings after cuttage, root is observed
Growing state, 3 cuttings are taken every time.
Turf, decomposed cow dung, vermiculite and perlite ratio (V/V) in the combination of 2 different cutting mediums of table
3.3 different cuttings handle the influence to cutting experiment
Experimental design is shown in Table 3,4, often handles cuttings 30,3 repetitions, using clear water as control, matrix is turf:Decomposed ox
Excrement:Vermiculite:Perlite=6:4:2:1;Cuttings a length of 12cm, 15cm and 18cm;Hormone selects methyl α-naphthyl acetate, ABT2 and indoles fourth
Acid;Concentration is 400mg/L, 800mg/L and 1200mg/L, processing time 2min.Random sampling after cuttage 1,8,15 and 22d, see
Examine cuttage rooting situation.
The orthogonal test factor level of table 3
The Orthogonal Experiment and Design of table 4
Influence of the 3.4 different cottage methods to cutting experiment
On this basis, matrix temperature differential method, control wet method, the temperature difference+control wet method, control etc. is respectively adopted and carries out fig cuttage
(temperature differential method realizes bottom and extraneous 12 DEG C of the temperature difference to nursery contrast test by spreading heating wire in substrate base;Control wet method passes through complete
Illumination spraying realization, water content 25%), it is shown in Table 5.30 cuttings, 3 repetitions are taken in each greenhouse.In cuttage 1,8,15 and 22d
Random sampling afterwards, observe situation of taking root.
5 different cottage methods of table
Test period is on May 30th, 2014, and 1d slotting machines pour permeable before cuttage, with 0.1% liquor potassic permanganate to slotting machine
Carry out disinfection, cuttings is cut to be sterilized to its lower end afterwards, cuttage after HORMONE TREATMENT.With being slightly coarser than the branch of cuttings by 20 × 15cm rows
Spacing in the rows jack on slotting machine, then cuttings is inserted in the hole, 2~3cm (containing 1 bud) is revealed in upper end.After cuttage, a kind is observed every 6d
Seedling callus situation, to wound healing after observe 1 secondary root situation weekly.It is managed using full exposure spraying, the after cuttage
45d, 60d and 75d, respectively spray foliar fertilizer 1 time, and foliar fertilizer is that proportioning is that N, P, K are followed successively by 300mg/L, 25mg/L and 50mg/L
Nutrient solution, and carry out Routine Management (Ji Hongwen Xinjiang greenhouse fig cuttage raise seedling technique [J] modern horticulturals, 2013,6:
29).On September 25th, 2014, statistics cuttage seedling rooting number, root length, rooting rate and survival rate etc., overall merit cuttage rooting
Quality (average root long × 25% of Q=rooting rates × 50%+ mean elements × 25%+), and using SPSS18.0 statistical softwares point
Analyse related data.
4 results and analysis
4.1 different cuttage greenhouses are taken root the influence of situation to fig
Influence of the 6 different greenhouses of table to cuttage root-taking
Note:Significant difference (p between different alphabetical expression processing<0.05), similarly hereinafter.
Table 6 show that the callus of different greenhouse fig cuttages and indefinite root growth ability are different, wherein optimizing
The cuttings callus apparition in type heliogreenhouse and lew is relatively short, significantly earlier than other processing;In vinyl house
Time of occurrence is at the latest.The cuttings adventitious root apparition is most short in Optimization-type heliogreenhouse, is secondly plain edition heliogreenhouse, and the two is aobvious
Write earlier than other processing;Time of occurrence is at the latest in lew.In addition, the callus rate of fig cuttage in Optimization-type heliogreenhouse
It is high with rooting rate, adventitious root is produced to cuttage and acts on very big, caused indefinite root long, and quality of rooting is high.Experiment is it is also shown that excellent
The effect of change type heliogreenhouse heat storing and heat preserving protrudes, and winter reduces heat and scattered and disappeared, and other season ventilation effects are uniform, are also equipped with preventing
Worm, waterproof, the features such as good appearance and big operating space.Therefore, fig cutting propagation is carried out in Optimization-type heliogreenhouse
Situation of taking root it is best, root system development is more early, is optimal greenhouse, should give large-scale demonstration popularization.At 4.2 different substrates
Manage the influence for situation of being taken root to fig
The different substrates of table 7 handle the influence of cuttage root-taking
As can be seen that the callus ability opposite sex of different substrates processing fig cuttage is larger, the cuttings of T5 processing is cured table 7
The injured tissue apparition is most short, only 8.67d, significantly earlier than other processing;T2 processing time of occurrence is 14.33d at the latest.It is different
Influence of the matrix treatments to the fig cuttage adventitious root apparition is similar to the influence to callus:The cuttings of T5 processing is indefinite
The root apparition is still most short, is 10d, is slightly sooner in time than T4 processing, but significantly earlier than other processing;The cuttings adventitious root of T1 processing occurs
Time phase is longer, is 19.33d;Although the cuttings of T2 processing has callus appearance, but adventitious root do not occur.In root long side
Face, adventitious root caused by T5 processing is most long, has reached 2.39cm, has been significantly higher than other processing;Tri- processing root longs of T1, T3, T4
Remain basically stable, otherness is not notable.T5 processing is maximum to the facilitation of cutting plantation, and rooting rate has reached 40.44%, shows
Work is higher than other processing;It is T4, T3 and T1 processing that other, which are followed successively by, and rooting rate is respectively 26.48%, 12.64% and 9.45%;
T2 processing is worst, adventitious root does not occur.In terms of quality of rooting, the quality of rooting of T5 processing is maximum, is 21.74, is secondly
T4 processing, and the quality of rooting of T2 processing is minimum.
Being drawn by table 7, the influence of the callus ability of different substrates processing fig cuttage has significant difference, wherein,
Turf:Decomposed cow dung:Vermiculite:Perlite=6:4:2:1st, the cuttings callus that the composite interstitial substance that bone meal content is 6% is handled
Apparition and adventitious root apparition are most short, considerably less than other processing, show the processing be fig cuttage tissue callus and
The optimum growh environment of indefinite root development.In addition, turf:Decomposed cow dung:Vermiculite:Perlite=6:4:2:1st, bone meal content is
The fig cuttage rooting of 6% composite interstitial substance processing is most long, and maximum to cutting plantation facilitation, quality of rooting is maximum,
Show that the processing creates suitable condition for fig root growth and development.
Influence of 4.3 different disposals to fig cuttage root-taking
4.3.1 to the influence of fig cuttage tissue callus
Under different disposal, fig callus situation is shown in Table 8, analysis shows, according to extreme difference (R) size, influences to be cured
The primary and secondary order of injured tissue apparition factor is hormone concentration>Hormone types>Cutting length, i.e. 15cm cuttings+1200mg/
LABT2.Similarly, influence cuttings callus rate factor primary and secondary order be:Cutting length>Hormone concentration>Hormone types, i.e. 18cm are inserted
Fringe+400mg/L methyl α-naphthyl acetates.
Directly perceived analysis of the table 8 to fig cuttage callus
Table 9 can be seen that earliest, the only 6.84d that the fig cuttage callus of the 5th group of processing occurs, significantly
Earlier than other processing;3rd group occurs the latest, having respectively reached 10.48d and 10.28d with the 6th group of processing callus.4th
The fig cuttage callus rate highest of group processing, has reached 76%, has been significantly higher than other processing;The callus rate time of 8th group of processing
It, is 68%, is significantly higher than other processing;7th group of processing cuttage callus rate is minimum, is 40.33%.Comprehensive analysis, cuttings
15cm combination 400mg/L methyl α-naphthyl acetates occur for promotion fig cuttage callus and the optimal conditions of growth.
The Multiple range test of the different disposal fig cuttage callus situation of table 9
4.3.2 to the influence of the indefinite root development of fig cuttage
Influence of the different disposal to fig cuttage root-taking situation, table 10 show, influence adventitious root apparition each factor most
Excellent water is put down as 12cm cuttings+1200mg/L methyl α-naphthyl acetates;It is 15cm cuttings+800mg/ to influence each factor optimal level of cuttings radical
LABT2;It is 15cm cuttings+1200mg/L ABT2 to cuttings root long effect each factor optimal level of size;Fig cuttage is given birth to
It is 15cm cuttings+800mg/L ABT2 that root rate, which influences each factor optimal level,;Each factor is influenceed on fig cuttage root-taking quality
Optimal level is 15cm cuttings+800mg/L ABT2.
The analysis directly perceived that table 10 influences on fig cuttage adventitious root
Different disposal is as shown in table 11 to fig cuttage root-taking orthogonal experiments, the fig cuttage of the 7th group of processing
Earliest, the only 9.26d that adventitious root occurs, earlier than the 5th group processing (9.34d), but both othernesses are not notable, and remove and the 3rd
Group and the 4th group of processing difference be not significantly outer, significantly earlier than other processing;2nd group, the 8th group of processing and the 9th group of processing adventitious root
What is occurred is later, respectively 13.97d, 13.85d and 14.54d;There is the adventitious root time at the latest in 6th group of processing, is 15.17d,
Significantly more than other processing;1st group of processing does not occur adventitious root, and correlation performance is worst.In terms of root long, the 6th group of processing
The elongation of adventitious root can most be promoted, cuttings adventitious root length has reached 3.28cm, has been all remarkably higher than other processing;5th group, the 4th
The adventitious root length of group processing and the 2nd group of processing is also longer, has respectively reached 2.6cm, 2.71cm and 2.43cm;9th group of processing
Root long it is shorter, only 0.85cm.The influence that different disposal produces radical to fig cuttage is also different.5th group of processing is not
Determining root, quantity occur most, have reached 26.33, have been significantly higher than other processing;Next to that the indefinite radical that the 7th group of processing occurs
Measure more, reached 6.27;3rd group and the 9th group of adventitious root caused by processing it is less, respectively 1.72 and 2.11, remove
Outside more than the 1st group not taken root, minimum number.
Found out by table 11, the fig cutting plantation of different disposal is widely different.The cutting plantation of 5th group of processing reaches
To 57.67%, it is significantly higher than other processing;Next to that the 4th group of processing, cutting plantation 36.33%;Other processing cuttages
All below 30%, the 3rd group and the 6th group is only 14.33% and 13% respectively, but is better than the 1st group of processing do not taken root rooting rate.
Rooting index can easily illustrate as an overall target and verify the complexity of plant establishment.In addition, the 5th
The cuttage root-taking quality highest of group processing, has reached 36.07, has been significantly higher than other processing;Secondly it is the 4th group of processing, matter of taking root
Measure as 19.51, in addition to the 1st group does not take root, the quality of rooting of the 8th group of processing is minimum, and only 9.
The Multiple range test of the indefinite root development of different disposal fig cuttage of table 11
The relation of 4.4 cottage methods and fig cuttage seedling rooting
Table 12 is as can be seen that fig seedling callus ability is different under different cottage methods, and wherein it is wet to combine control for the temperature difference
The cuttings callus apparition of method processing is most short, and only 6.14d, single use temperature differential method time of occurrence is 10.44d at the latest.
Different cottage method epicormic branch cuttage callus rates are there is also significant difference, the cuttage callus rate highest of single use temperature differential method processing,
Reach 69%, influence least favorable of the single use control wet method to cuttage, only 40.67%, it is substantially less than other processing.Examination
To test and show, the indefinite root growth situation of distinct methods cuttage is different, and single use control wet method produces adventitious root effect maximum to cuttage,
The cuttings adventitious root apparition is most short, is 8.85d, and control treatment is taken second place, and is 9.34d, the two is significantly earlier than other processing;Thermal junction
It is most to close adventitious root caused by control wet process cuttings, is 32.46, is significantly higher than other methods, each cuttings of temperature differential method only has
18.69 bar adventitious root.In terms of root long, temperature difference adventitious root average length highest with reference to caused by control wet method, 3.06cm is reached, has shown
Work is higher than other method.In addition, to combine control wet process maximum to the facilitation of cuttage root-taking for the temperature difference, rooting rate up to 64.33%,
Temperature differential method rooting rate is minimum.In terms of quality of rooting, the temperature difference combines control wet method quality of rooting maximum, is 41.05, and the life of temperature differential method
Root quality is minimum, and only 27.71.
The relation of the cottage method of table 12 and fig cuttage seedling rooting
Embodiment 1
This experiment is carried out in our hospital's scientific base fig Germplasm Resources Optimization-type heliogreenhouse, Growing season greenhouse temperature
15-22 DEG C, humidity 70%~90%, sunshine is sufficient, and the content of organic matter is high in test material, meta-acid.Cuttage in cuttage slotting machine
Substrate base spreads heating wire, realizes bottom and extraneous 12 DEG C of the temperature difference.
Cuttings is ' Japanese purple fruit ' fig robust growth, organizes substantial semi-lignified branch raw then, and length is
15cm, each 2~3 leaves of cuttings band, upper end are whittled into horse ear shape inclined-plane, cuttings is cut away from truncate at terminal bud 2cm at the axillary bud of lower end
12min is soaked in its lower end in 800mg/L carbendazim thimerosals afterwards, utilizes 800mg/L ABT2 promoter immersion treatments 2min.
The composite interstitial substance that cutting medium mixes for turf, decomposed cow dung, vermiculite, perlite and bone meal, turf, corruption
The volume ratio of ripe cow dung, vermiculite and perlite is 6:4:2:1st, bone meal accounts for the 6% of cutting medium cumulative volume, with 4000mg/L network ammonia
Copper, 160mg/L hymexazols and the sterilization of 1200mg/L carbendazim are mixed thoroughly.May 30, i.e., 1d slotting machines poured permeable before cuttage, used
0.1% liquor potassic permanganate carries out disinfection to slotting machine, with being slightly coarser than the branch of cuttings by 20cm × 15cm distances between rows and hills on slotting machine
Jack, then the cuttings handled well is inserted in the hole, 2~3cm (containing 1 bud) is revealed in upper end.It is managed, is made using full exposure spraying
Water content 25% in greenhouse, it is 95% (cuttings starts germination and is considered as cutting survival) that 30d, which counts survival rate, after cuttage, 45d,
60d and 75d, respectively sprays foliar fertilizer 1 time, and foliar fertilizer is that proportioning is the battalion that N, P, K are followed successively by 300mg/L, 25mg/L and 50mg/L
Nutrient solution, and carry out Routine Management (Ji Hongwen Xinjiang greenhouse fig cuttage raise seedling technique [J] modern horticulturals, 2013,6:29).
September 25 days, obtain height of seedling (aerial part) 75~85cm, 1.0~1.5cm of base diameter cuttage seeding, you can go out garden transplanting.System
Count cuttage seedling rooting number, root length, rooting rate etc., overall merit cuttage rooting quality (Q=rooting rates × 50%+ average roots
Average root long × 25% of number × 25%+), and using SPSS18.0 statistical softwares analysis related data.
The index parameters such as cuttage seedling rooting number, root long, rooting rate and the survival rate that the method that the present invention optimizes obtains, enter
And the quality of rooting of overall merit cuttings.
The present invention of table 13 optimizes the growing state of fig cuttage seeding under treatment conditions
Claims (7)
1. a kind of greenhouse fig cutting propagation method for culturing seedlings, it is characterised in that comprise the steps of:
(1) it is that the fig branch for giving birth to semi-lignified then that 15cm robust growths, tissue enrich is cuttings to take length, each to insert
2~3 leaves of braid, upper end are whittled into horse ear shape inclined-plane away from truncate at terminal bud 2cm at the axillary bud of lower end;
(2) sterilizing processing is carried out to cuttings, reuses 800mg/L ABT2 promoter immersion treatments 2min;
(3) in Optimization-type heliogreenhouse, the cutting medium bottom paving heating wire in slotting machine, substrate base and external world's temperature are realized
Poor 10~15 DEG C, the cuttings that previous step is handled well inserts cutting medium, and 2~3cm is revealed in cuttings upper end, containing 1 bud;Wherein, institute
The cutting medium stated be turf, decomposed cow dung, vermiculite, perlite and bone meal mix, described turf, decomposed cow dung,
The volume ratio of vermiculite and perlite is 6:4:2:1, described bone meal accounts for the 6% of cutting medium cumulative volume;
(4) after cuttage, sprayed and managed using full exposure, make water content 20~25% in greenhouse;45d, 60d and 75d after cuttage,
Respectively spray foliar fertilizer 1 time, carry out conventional field management;Cultivate 3~4 months and obtain cuttage seeding;Described foliar fertilizer is that proportioning is
N, P, K are followed successively by 300mg/L, 25mg/L and 50mg/L nutrient solution;
Optimization-type heliogreenhouse is included between rear wall, gable, rear roofing, front roof, front roof bow member, greenhouse front foot and buffering;Institute
The rear wall stated include it is fixed after wall and wall support structure, after described fixation wall by it is interior include outward recuperation layer, thermal insulation layer and
Heat-insulation layer;Described recuperation layer is piled up by the building-block filled with clayed soil to be formed, and described thermal insulation layer is styrofoam, institute
The heat-insulation layer stated is piled up by the building-block filled with stalk block to be formed;After dismounting being provided with described wall support structure
Wall, wall is piled up by stalk block and formed after described dismounting;The inner side of wall is covered with fly net after described dismounting, wall after dismounting
Outer side covers have the pull-type plastic sheeting of volume;Described wall support structure includes inner column, outer column, crossbeam, longeron and lacing wire,
The inner column arranges at one that outer column dislocation is arranged in two adjacent inner columns along rear wall of greenhouse length interval per 4m
Between;Described front roof bow member is coating steel pipe fabricated construction, and front roof bow member includes top boom, lower boom, connector, on
Chord member and lower boom are hinged to assembled bow member by connector, and the both ends of top boom and lower boom, which are respectively articulated with, is fixed on greenhouse
Wall top end after front foot and dismounting;Top portion ventilation window and sidepiece ventilation are respectively equipped with the top of described front roof bow member and sidepiece
Window, described top portion ventilation window and sidepiece vent window are provided with fly net, the trend of top portion ventilation window and sidepiece vent window with
Greenhouse it is horizontally-parallel, width is 80~100cm;On described front roof bow member in addition to top sky air regulator and sidepiece vent window
Compressed covered with canopy film, and with film-pressing line;Described rear roofing is by from interior single-faced color steel plate, the expansion polyphenyl set gradually outward
Plate, cement, flyash haydites of book structure screed-coat, SBS asphalt felts waterproof layer, cement mortar protective course are formed;Between described buffering
The west side of wall after the fixation of heliogreenhouse.
2. according to the method for claim 1, it is characterised in that cuttings carries out sterilizing processing and is:Cuttings cut after under it
End 10~15min of immersion treatment in 800~1000mg/L carbendazim thimerosals.
3. according to the method for claim 2, it is characterised in that its lower end is in 800mg/L carbendazim thimerosals after cuttings is cut
Middle immersion 12min.
4. according to the method for claim 1, it is characterised in that before cutting medium cuttage using 4000mg/L network ammonia copper,
160mg/L hymexazols and the sterilization of 1200mg/L carbendazim are mixed thoroughly stand-by;1d slotting machines pour permeable before cuttage, with 0.1% potassium permanganate
Solution carries out disinfection to slotting machine.
5. according to the method for claim 1, it is characterised in that sprayed and managed using full exposure, make water content in greenhouse
25%.
6. according to the method for claim 1, it is characterised in that the distance between rows and hills of cuttage is 20 × 15cm in step (3).
7. according to the method for claim 1, it is characterised in that described cuttage seeding is the high 75~85cm of aerial part, base portion
1.0~1.5cm of diameter cuttage seeding.
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