CN105830716A - Cutting seedling raising method for Antiaris toxicaria - Google Patents

Cutting seedling raising method for Antiaris toxicaria Download PDF

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Publication number
CN105830716A
CN105830716A CN201610218658.9A CN201610218658A CN105830716A CN 105830716 A CN105830716 A CN 105830716A CN 201610218658 A CN201610218658 A CN 201610218658A CN 105830716 A CN105830716 A CN 105830716A
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Prior art keywords
cutting
cuttage
root
curare
wood
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CN201610218658.9A
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Chinese (zh)
Inventor
王亚波
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Chang Zhourun Reaches Scientific And Technological Information Advisory Co Ltd
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Chang Zhourun Reaches Scientific And Technological Information Advisory Co Ltd
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Priority to CN201610218658.9A priority Critical patent/CN105830716A/en
Publication of CN105830716A publication Critical patent/CN105830716A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

Abstract

The invention relates to a cutting seedling raising method for Antiaris toxicaria. The method comprises steps as follows: (1) preparation of a cutting medium; (2) preparation of a rooting agent; (3) material selection and cutting; (4) management and transplantation after cutting. Annual semi-lignified young and tender Antiaris toxicaria branches which are 0.6-0.8 cm thick and 12-16 cm long are taken as cuttings, each cutting has two leaves, positions, 3-5 cm away from terminal buds, of upper ends are truncated, axillary bud positions of lower ends are cut into horse-ear-shaped slopes, and sections at upper and lower ends are inserted into the prepared cutting medium after cutting pretreatment. With the adoption of the cutting seedling raising method, fine properties of mother plants of Antiaris toxicaria can be kept, the propagation coefficient is high and more than 90% higher than that of a conventional root burying seedling raising method, the cutting technology is simple to operate and convenient to popularize, and the cost is lower than that of tissue culture.

Description

A kind of curare wood cuttage and seedling culture method
Technical field
The present invention relates to curare wood breeding field, particularly relate to a kind of curare wood cuttage and seedling culture method.
Background technology
Curare wood, has another name called Radix aconiti hemsleyani, is born in the rainforest of height above sea level less than 1500 meters, arbor, high 25-40 more Rice, diameter of a cross-section of a tree trunk 1.3 meters above the ground 30-40 centimetre;Tool milky resin, bark Lycoperdon polymorphum Vitt, bloom spring, be that country's three-level protective is planted Thing, is a kind of severe toxicity plant and medicinal plants, and the ethanol extraction of curare wood milk has heart tonifying, boosts and increase Add the effects such as cardiac output.Protection has been strengthened in Guangdong, the place of production of Wei Jian protection zone, Guangxi, expands plantation.
The method that existing curare wood propagation method is mainly seeding and seedling raising, and in order to keep plant original excellent Optimum shape, needs to use the asexual reproduction method expanding propagation such as cuttage, press strip, group training, group training to breed cost height, But there is not been reported to utilize cottage method breeding curare wood.
Summary of the invention
In order to solve above-mentioned technical problem, it is provided that a kind of technical scheme utilizing cottage method breeding curare wood, The present invention provides techniques below scheme:
A kind of curare wood cuttage and seedling culture method, comprises the following steps:
(1), preparation cutting medium: Vermiculitum, peat soil, wormcast, Crinis Carbonisatus are pressed 1:1:1:0.05 mixing Forming cutting medium, burning sun was exposed to the sun after 3-5 day, and to make the slotting machine that thickness is 12cm standby;
Owing to Vermiculitum has the ability that ion exchanges, the nutrition to soil has effect greatly;Peat soil contains rich Rich humus, wormcast is as supplementary various demands and the nutrition that can meet plant growing all sidedly of nutriment in a fertilizer Composition and do not result in burn seedlings;Crinis Carbonisatus is the carbide that Crinis Carbonisatus is made, containing substantial amounts of trace element, suitable Amount makes an addition to can significantly improve in cutting medium cuttage survival rate.Above-mentioned cooperating of 4 kinds of compositions can Meet the nutritional need of curare wood cuttage and cutting medium pH value can be made to be maintained between 6.8-7.7, and comparing Conventional perlite can be greatly improved, with the compatibility of peat soil, the cuttage survival rate that curare is wooden, increases root system breeding Coefficient.
(2), root-growing agent is prepared: root-growing agent is made up of indolebutyric acid, dehydrated alcohol, Oleum menthae, corn starch, Described indolebutyric acid concentration is 400-600mg/L, dehydrated alcohol volumetric concentration is 50%, Oleum menthae concentration is 100-200mg/L, corn starch 20-30g/L;
The indolebutyric acid using higher concentration can promote Rapid Rooting;On the one hand the dehydrated alcohol of 50% can carry The penetrating power of high root-growing agent, on the other hand is used for dissolving Oleum menthae;A small amount of Oleum menthae energy is added at root-growing agent Enough accelerate the microcirculation of cutting bottom wound surface, accelerate rooting rate;The addition of corn starch makes whole taking root Agent solution presents thin slurry shape, after cutting bottom dips root-growing agent, it is possible to form a wound surface in bottom Protecting film, pins the milk within cutting, it is possible to increase survive the speed of growth of rear cutting.
(3), selection and cuttage: choose rugosity be 0.6-0.8cm, the most raw curare of a length of 12-16cm wood half Lignifying edible tender branch is cutting, and 2 leaves of each cutting band, upper end is truncate at terminal bud 3-5cm, lower end Being whittled into horse ear shape inclined-plane at axillalry bud, upper end section smears the Triiodobenzoic acid of 0.0001%, and lower end dips step (2) The root-growing agent of middle preparation, the time of dipping is 3-5s;The cutting handled well is inserted perpendicularly in cutting medium, skewer The slotting degree of depth is the 1/3-1/2 of cutting length;
The Triiodobenzoic acid of low concentration is smeared as plant growth inhibitor for suppressing plant to push up in cutting upper surface End advantage so that auxin moves to promote the growth of lateral bud, tiller and root system to cutting bottom.Under cutting End dips the root-growing agent of preparation in step (2) can improve cutting survival rate, due to inserting of selecting in this method Fringe is for giving birth to twig then, and therefore to dip the time unsuitable long for root-growing agent, prevents burn seedlings, experiment proof from dipping 3-5s Time cutting survival rate is the highest, root system breeding coefficient is the highest.
(4) manage and transplant after, inserting: slotting machine indoor temperature controls at 25-30 DEG C, and keeps water content of substrate all the time More than 20%, holding indoor relative humidity is 60%-70%, carries out after carrying out the field management 3-4 month of routine Transplant.Owing to curare wood is suitable raw and Tropical rain forest, average annual temperature is high, humidity is high, and therefore cuttage room to keep Higher room temperature and humidity.
Further, the cutting medium that described step (1) is prepared need to water permeable in cuttage and disappear the previous day Poison processes, and described disinfection treatment method is the Bravo solution of sprinkling 800-1000 times.
Further, ultrasonic generator is used to process after cutting lower end dips root-growing agent in described step (3) Cutting, is first ultrasonic Treatment 5-10s of 300-400W with power, reduces power afterwards, uses 100-200W Power Processing 30-60s, utilize ultrasonic Treatment can increase the permeability of cell, use suitable ultrasonic Ripple processes curare wood cutting can produce certain stimulation to cutting, thus beneficially exogenous auxin enters Cell interior, the differentiation of induction root restriction and the formation of adventitious root, improve the germination percentage of cutting, transplant survival The speed of growth of early stage after rate and cuttage.The present invention first with after the of short duration process cutting of powerful ultrasound wave, Reduce again ultrasonic power carry out once for time longer process, large power supersonic process can instantaneous breakthrough thin After birth, increases its permeability, the improvement branch physiology shape that small-power supersound process then can be the softest from inside State, processes cutting by supersound process and auxin simultaneously and can significantly improve cuttage survival rate.
Further, in described step (3), the seeding row spacing of cuttage is 20 × 15cm.
The beneficial effects of the present invention is:
(1), cutting medium collocation rationally, heat and moisture preserving and permeability good, every kilogram of content of organic matter is 350-450g, N P and K total amount are 40-60g, and pH value 6.8-7.7 has higher nutritive value, for branch The growth of bar provides good basis;
(2), homemade root-growing agent formula can be greatly improved cuttage survival rate.
(3), by the combination of root-growing agent Yu ultrasonic Treatment, curare wood cutting callus and adventitious root are made Apparition shorten, especially the most favourable to cutting seedling callus, moreover it is possible to improve cutting seedling take root number, root length, Rooting rate and Rooting index.
(4), this method cultivate curare wood cutting seedling survival rate more than 95%, garden can be gone out then, can be real Cuttage the most then.
Detailed description of the invention
Embodiment 1,
A kind of curare wood cuttage and seedling culture method, comprises the following steps:
(1), preparation cutting medium: Vermiculitum, peat soil, wormcast, Crinis Carbonisatus are pressed 1:1:1:0.05 and mixed shape Becoming cutting medium, burning sun was exposed to the sun after 3-5 day, and to make the slotting machine that thickness is 12cm standby;Need the previous day in cuttage Watering permeable and disinfect, described disinfection treatment method is the Bravo solution of sprinkling 800-1000 times.
(2), root-growing agent is prepared: root-growing agent is made up of indolebutyric acid, dehydrated alcohol, Oleum menthae, corn starch, Described indolebutyric acid concentration is 400mg/L, dehydrated alcohol volumetric concentration is 50%, Oleum menthae concentration is 100mg/L, Corn starch 20g/L;
(3), selection and cuttage: choose rugosity be 0.6-0.8cm, the most raw curare of a length of 12-16cm wood half Lignifying edible tender branch is cutting, and 2 leaves of each cutting band, upper end is truncate at terminal bud 3-5cm, lower end Being whittled into horse ear shape inclined-plane at axillalry bud, upper end section smears the Triiodobenzoic acid of 0.0001%, and lower end dips step (2) The root-growing agent of middle preparation, the time of dipping is 3-5s;The cutting handled well is inserted perpendicularly in cutting medium, skewer The slotting degree of depth is the 1/3-1/2 of cutting length;Cuttage seeding row spacing is 20 × 15cm.
(4) manage and transplant after, inserting: slotting machine indoor temperature controls at 25-30 DEG C, and keeps water content of substrate all the time More than 20%, holding indoor relative humidity is 60%-70%, carries out after carrying out the field management 3-4 month of routine Transplant.
Embodiment 2,
A kind of curare wood cuttage and seedling culture method, comprises the following steps:
(1), preparation cutting medium: Vermiculitum, peat soil, wormcast, Crinis Carbonisatus are pressed 1:1:1:0.05 and mixed shape Becoming cutting medium, burning sun was exposed to the sun after 3-5 day, and to make the slotting machine that thickness is 12cm standby;Need the previous day in cuttage Watering permeable and disinfect, described disinfection treatment method is the Bravo solution of sprinkling 800-1000 times.
(2), root-growing agent is prepared: root-growing agent is made up of indolebutyric acid, dehydrated alcohol, Oleum menthae, corn starch, Described indolebutyric acid concentration is 600mg/L, dehydrated alcohol volumetric concentration is 50%, Oleum menthae concentration is 150mg/L, Corn starch 30g/L;
(3), selection and cuttage: choose rugosity be 0.6-0.8cm, the most raw curare of a length of 12-16cm wood half Lignifying edible tender branch is cutting, and 2 leaves of each cutting band, upper end is truncate at terminal bud 3-5cm, lower end Being whittled into horse ear shape inclined-plane at axillalry bud, upper end section smears the Triiodobenzoic acid of 0.0001%, and lower end dips step (2) The root-growing agent of middle preparation, the time of dipping is 3-5s;The cutting handled well is inserted perpendicularly in cutting medium, skewer The slotting degree of depth is the 1/3-1/2 of cutting length;The seeding row spacing of cuttage is 20 × 15cm.
(4) manage and transplant after, inserting: slotting machine indoor temperature controls at 25-30 DEG C, and keeps water content of substrate all the time More than 20%, holding indoor relative humidity is 60%-70%, carries out after carrying out the field management 3-4 month of routine Transplant.
Embodiment 3,
A kind of curare wood cuttage and seedling culture method, comprises the following steps:
(1), preparation cutting medium: Vermiculitum, peat soil, wormcast, Crinis Carbonisatus are pressed 1:1:1:0.05 and mixed shape Becoming cutting medium, burning sun was exposed to the sun after 3-5 day, and to make the slotting machine that thickness is 12cm standby;Need the previous day in cuttage Watering permeable and disinfect, described disinfection treatment method is the Bravo solution of sprinkling 800-1000 times.
(2), root-growing agent is prepared: root-growing agent is made up of indolebutyric acid, dehydrated alcohol, Oleum menthae, corn starch, Described indolebutyric acid concentration is 400mg/L, dehydrated alcohol volumetric concentration is 50%, Oleum menthae concentration is 200mg/L, Corn starch 30g/L;
(3), selection and cuttage: choose rugosity be 0.6-0.8cm, the most raw curare of a length of 12-16cm wood half Lignifying edible tender branch is cutting, and 2 leaves of each cutting band, upper end is truncate at terminal bud 3-5cm, lower end Being whittled into horse ear shape inclined-plane at axillalry bud, upper end section smears the Triiodobenzoic acid of 0.0001%, and lower end dips step (2) The root-growing agent of middle preparation, the time of dipping is 3-5s;Use ultrasonic generator to process cutting again, first use power For ultrasonic Treatment 5s of 400W, reduce power afterwards, use Power Processing 60s of 100W;To handle well Cutting be inserted perpendicularly in cutting medium, the cuttage degree of depth is the 1/3-1/2 of cutting length;The seeding row spacing of cuttage is 20×15cm。
(4) manage and transplant after, inserting: slotting machine indoor temperature controls at 25-30 DEG C, and keeps water content of substrate all the time More than 20%, holding indoor relative humidity is 60%-70%, carries out after carrying out the field management 3-4 month of routine Transplant.
Following table is the different embodiment impacts on cuttage root-taking.
By upper table analysis, use the method for the present invention carry out curare wood its wound healing rate of cutting propagation, The indexs such as adventitious root apparition, quantity of taking root, root length, rooting rate, cuttage survival rate are all significantly better than Commonly bury root method for culturing seedlings, and wherein embodiment 3 is optimized cuttage processing method.
With above-mentioned foundation desirable embodiment of the present invention for enlightenment, by above-mentioned description, related work people Member can carry out various change and amendment completely in the range of without departing from this invention technological thought. The content that the technical scope of this invention is not limited in description, it is necessary to according to right Determine its technical scope.

Claims (4)

1. a curare wood cuttage and seedling culture method, it is characterised in that comprise the following steps:
(1) preparation cutting medium: Vermiculitum, peat soil, wormcast, Crinis Carbonisatus are pressed 1:1:1:0.05 and mixed shape Becoming cutting medium, burning sun was exposed to the sun after 3-5 day, and to make the slotting machine that thickness is 12cm standby;
(2) preparation root-growing agent: root-growing agent is made up of indolebutyric acid, dehydrated alcohol, Oleum menthae, corn starch, Described indolebutyric acid concentration is 400-600mg/L, dehydrated alcohol volumetric concentration is 50%, Oleum menthae concentration is 100-200mg/L, corn starch 20-30g/L;
(3) selection and cuttage: choose rugosity be 0.6-0.8cm, the most raw curare of a length of 12-16cm wood half Lignifying edible tender branch is cutting, and 2 leaves of each cutting band, upper end is truncate at terminal bud 3-5cm, lower end Being whittled into horse ear shape inclined-plane at axillalry bud, upper end section smears the Triiodobenzoic acid of 0.0001%, and lower end dips step (2) root-growing agent of preparation in, the time of dipping is 3-5s;The cutting handled well is inserted perpendicularly into cutting medium In, the cuttage degree of depth is the 1/3-1/2 of cutting length;
(4) manage and transplant after inserting: slotting machine indoor temperature controls at 25-30 DEG C, and keeps water content of substrate all the time More than 20%, holding indoor relative humidity is 60%-70%, and the field management 3-4 month carrying out routine is laggard Row is transplanted.
2. a kind of curare wood cuttage and seedling culture method as claimed in claim 1, it is characterised in that: described step (1) cutting medium prepared need to water permeable in cuttage and disinfect the previous day, described in disinfect Method is the Bravo solution of sprinkling 800-1000 times.
3. a kind of curare wood cuttage and seedling culture method as claimed in claim 1, it is characterised in that: described step (3) use ultrasonic generator to process cutting after cutting lower end dips root-growing agent in, with power be first Ultrasonic Treatment 5-10s of 300-400W, reduces power afterwards, uses Power Processing 30-60s of 100-200W.
4. a kind of curare wood cuttage and seedling culture method as claimed in claim 1, it is characterised in that: described step (3) in, the seeding row spacing of cuttage is 20 × 15cm.
CN201610218658.9A 2016-04-08 2016-04-08 Cutting seedling raising method for Antiaris toxicaria Pending CN105830716A (en)

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CN108834708A (en) * 2018-06-12 2018-11-20 浦江县元寿农业科技有限公司 The propagation method of Chinese ilex
CN114009438A (en) * 2021-11-03 2022-02-08 江苏省中国科学院植物研究所 Preparation for improving survival rate of color-leaf poplar and application

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108834708A (en) * 2018-06-12 2018-11-20 浦江县元寿农业科技有限公司 The propagation method of Chinese ilex
CN114009438A (en) * 2021-11-03 2022-02-08 江苏省中国科学院植物研究所 Preparation for improving survival rate of color-leaf poplar and application

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