CN104160023B - 热稳定性得到改善的d‑阿洛酮糖3‑差向异构酶突变体和使用其的d‑阿洛酮糖的连续制备 - Google Patents
热稳定性得到改善的d‑阿洛酮糖3‑差向异构酶突变体和使用其的d‑阿洛酮糖的连续制备 Download PDFInfo
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- CN104160023B CN104160023B CN201280052112.0A CN201280052112A CN104160023B CN 104160023 B CN104160023 B CN 104160023B CN 201280052112 A CN201280052112 A CN 201280052112A CN 104160023 B CN104160023 B CN 104160023B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
- C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/24—Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y501/00—Racemaces and epimerases (5.1)
- C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
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- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
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- General Health & Medical Sciences (AREA)
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- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2011-0084712 | 2011-08-24 | ||
| KR1020110084712A KR101203856B1 (ko) | 2011-08-24 | 2011-08-24 | 열 안정성이 향상된 사이코스 에피머화 효소 변이체 및 이를 이용한 사이코스의 연속적 생산 |
| PCT/KR2012/006637 WO2013027999A2 (ko) | 2011-08-24 | 2012-08-21 | 열 안정성이 향상된 사이코스 에피머화 효소 변이체 및 이를 이용한 사이코스의 연속적 생산 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104160023A CN104160023A (zh) | 2014-11-19 |
| CN104160023B true CN104160023B (zh) | 2017-03-29 |
Family
ID=47565145
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201280052112.0A Active CN104160023B (zh) | 2011-08-24 | 2012-08-21 | 热稳定性得到改善的d‑阿洛酮糖3‑差向异构酶突变体和使用其的d‑阿洛酮糖的连续制备 |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US9217166B2 (https=) |
| EP (2) | EP2749645B1 (https=) |
| JP (1) | JP6043354B2 (https=) |
| KR (1) | KR101203856B1 (https=) |
| CN (1) | CN104160023B (https=) |
| DK (1) | DK2749645T3 (https=) |
| ES (1) | ES2626501T3 (https=) |
| HR (1) | HRP20170813T1 (https=) |
| HU (1) | HUE035316T2 (https=) |
| IN (1) | IN2014CN01614A (https=) |
| PL (1) | PL2749645T3 (https=) |
| PT (1) | PT2749645T (https=) |
| RS (1) | RS56088B1 (https=) |
| SI (1) | SI2749645T1 (https=) |
| WO (1) | WO2013027999A2 (https=) |
Families Citing this family (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101318422B1 (ko) * | 2013-04-09 | 2013-10-15 | 주식회사 삼양제넥스 | D-사이코스 에피머화 효소, 및 이를 이용하는 사이코스 생산방법 |
| KR101455624B1 (ko) | 2013-04-12 | 2014-10-28 | 주식회사한국야쿠르트 | 기능성 희귀당 사이코스의 생산능을 지닌 신규 클로스트리디움 볼티에 유래의 사이코스-3-에피머화 효소 및 이를 이용한 사이코스 생산방법 |
| KR101455759B1 (ko) * | 2013-04-23 | 2014-10-28 | 씨제이제일제당(주) | 사이코스 에피머화 효소 변이체 및 이를 이용한 사이코스의 제조 방법 |
| KR102189458B1 (ko) * | 2013-09-03 | 2020-12-11 | 로께뜨프레르 | D-사이코스 3-에피머라제의 개선된 변이체 및 그의 용도 |
| EP2843044A1 (en) * | 2013-09-03 | 2015-03-04 | Roquette Frères | Improved variant of D-psicose 3-epimerase and uses thereof |
| KR101539097B1 (ko) | 2013-12-26 | 2015-07-23 | 주식회사 삼양제넥스 | 사이코스 에피머화 효소를 암호화 하는 폴리뉴클레오타이드, 및 이를 이용하는 사이코스 생산방법 |
| KR101473918B1 (ko) | 2014-05-28 | 2014-12-17 | 대상 주식회사 | 사이코스 에피머화 효소, 이의 제조방법 및 이를 이용한 사이코스의 제조방법 |
| KR101577147B1 (ko) | 2014-10-01 | 2015-12-11 | 경상대학교산학협력단 | 사이코스의 생산 방법 |
| KR101607633B1 (ko) | 2014-10-21 | 2016-04-11 | 주식회사 삼양사 | 코리네박테리움속 균주를 이용한 사이코스 생산방법 |
| US10240140B2 (en) | 2014-10-30 | 2019-03-26 | Samyang Corporation | Expression system for psicose epimerase and production for psicose using the same |
| CN107109443B (zh) | 2014-11-06 | 2021-11-02 | 庆尚大学校产学协力团 | 阿洛酮糖的制备方法 |
| KR102044957B1 (ko) * | 2014-12-31 | 2019-11-14 | 주식회사 삼양사 | 사이코스의 제조방법 |
| KR101677368B1 (ko) | 2015-04-02 | 2016-11-18 | 씨제이제일제당 (주) | 신규 프로모터 및 이의 용도 |
| KR102087396B1 (ko) | 2015-11-16 | 2020-03-10 | 주식회사 삼양사 | 과당-함유 기질로부터 사이코스를 생산하는 방법 |
| KR101807507B1 (ko) | 2015-12-23 | 2017-12-12 | 씨제이제일제당(주) | D-사이코스 3-에피머화 효소 및 염을 포함하는 d-사이코스 제조용 조성물 및 이를 이용한 d-사이코스의 제조 방법 |
| KR101723007B1 (ko) | 2016-02-29 | 2017-04-04 | 씨제이제일제당(주) | 고순도 d-사이코스를 제조하는 방법 |
| CN105821027B (zh) | 2016-04-01 | 2023-11-21 | 南京朗奈生物技术有限公司 | 一种3-差向异构酶的用途 |
| KR101946157B1 (ko) * | 2016-07-29 | 2019-02-11 | 씨제이제일제당 (주) | D-타가토스 전환 활성이 향상된 헥수론산 c4-에피머화 효소 변이체 및 이를 이용한 d-타가토스의 제조 방법 |
| CN106148311B (zh) * | 2016-09-12 | 2019-06-21 | 上海立足生物科技有限公司 | 一种d-阿洛酮糖-3-差向异构酶的突变体及其应用 |
| KR101919713B1 (ko) * | 2016-11-16 | 2018-11-19 | 씨제이제일제당 (주) | 신규한 d-사이코스 3-에피머화 효소 및 이를 이용한 d-사이코스의 제조 방법 |
| CN108239633B (zh) * | 2016-12-26 | 2023-05-05 | 上海立足生物科技有限公司 | 一种催化活性得到提高的d-阿洛酮糖-3-差向异构酶的突变体及其应用 |
| WO2018129275A1 (en) | 2017-01-06 | 2018-07-12 | Greenlight Biosciences, Inc. | Cell-free production of sugars |
| CA3088598C (en) * | 2018-01-24 | 2021-03-30 | Matsutani Chemical Industry Co., Ltd. | Ketose 3-epimerase with improved thermal stability |
| KR102439295B1 (ko) | 2018-11-30 | 2022-09-02 | 씨제이제일제당 주식회사 | D-사이코스 결정 및 이의 제조 방법 |
| CN110396513B (zh) * | 2019-07-19 | 2022-01-11 | 天津科技大学 | 一种d-阿洛酮糖-3-差向异构酶的突变体及其应用 |
| CN110684762B (zh) * | 2019-11-12 | 2021-03-19 | 南京工业大学 | 一种d-阿洛酮糖-3-差向异构酶突变体及其编码基因、重组载体、重组菌株和应用 |
| CN111019928B (zh) * | 2019-12-11 | 2022-08-16 | 吉林中粮生化有限公司 | D-阿洛酮糖3-差向异构酶的编码基因、载体、重组细胞以及它们的应用 |
| KR102254411B1 (ko) | 2019-12-19 | 2021-05-24 | 대상 주식회사 | 알룰로스 에피머화 효소 변이체, 이의 제조방법 및 이를 이용한 알룰로스의 제조방법 |
| WO2021199445A1 (en) | 2020-04-03 | 2021-10-07 | Rensselaer Polytechnic Institute | Method for producing sulfated polysaccharide and method for producing paps |
| KR102448351B1 (ko) | 2020-04-27 | 2022-09-28 | 대상 주식회사 | 알룰로스 에피머화 효소 변이체, 이의 제조방법 및 이를 이용한 알룰로스의 제조방법 |
| US20220340944A1 (en) | 2020-06-03 | 2022-10-27 | Tianjin Institute Of Industrial Biotechnology, Chinese Academy Of Sciences | Allulose 3-epimerase mutant, engineered bacterium expressing same, and immobilized enzyme and immobilization method thereof |
| CN111793616B (zh) * | 2020-08-07 | 2022-04-12 | 天津科技大学 | 一种差向异构酶的突变体及其应用 |
| KR102682846B1 (ko) | 2021-11-19 | 2024-07-08 | 대상 주식회사 | 열 안정성이 우수한 알룰로스 에피머화 효소 변이체, 이의 제조방법 및 이를 이용한 알룰로스의 제조방법 |
| KR20240108871A (ko) * | 2022-12-30 | 2024-07-10 | 주식회사 삼양사 | 알룰로스 3-에피머화 변이 효소 및 이의 용도 |
| WO2025111274A2 (en) * | 2023-11-20 | 2025-05-30 | Novozymes A/S | Epimerase variants and polynucleotides encoding same |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101189332B (zh) * | 2005-06-01 | 2011-02-16 | Cj第一制糖株式会社 | 利用d-阿洛酮糖差向异构酶的d-阿洛酮糖生产方法 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0512150D0 (en) * | 2005-06-15 | 2005-07-20 | Scottish Crop Res Inst | Production of L-ascorbic acid |
| KR20110035805A (ko) * | 2009-09-30 | 2011-04-06 | 씨제이제일제당 (주) | 사이코스-에피머화 효소의 고정화 및 이를 이용한 사이코스의 제조방법 |
-
2011
- 2011-08-24 KR KR1020110084712A patent/KR101203856B1/ko active Active
-
2012
- 2012-08-21 CN CN201280052112.0A patent/CN104160023B/zh active Active
- 2012-08-21 RS RS20170604A patent/RS56088B1/sr unknown
- 2012-08-21 SI SI201230996T patent/SI2749645T1/sl unknown
- 2012-08-21 PT PT128260726T patent/PT2749645T/pt unknown
- 2012-08-21 IN IN1614CHN2014 patent/IN2014CN01614A/en unknown
- 2012-08-21 US US14/240,647 patent/US9217166B2/en active Active
- 2012-08-21 JP JP2014527066A patent/JP6043354B2/ja active Active
- 2012-08-21 HU HUE12826072A patent/HUE035316T2/en unknown
- 2012-08-21 EP EP12826072.6A patent/EP2749645B1/en active Active
- 2012-08-21 WO PCT/KR2012/006637 patent/WO2013027999A2/ko not_active Ceased
- 2012-08-21 ES ES12826072.6T patent/ES2626501T3/es active Active
- 2012-08-21 DK DK12826072.6T patent/DK2749645T3/en active
- 2012-08-21 PL PL12826072T patent/PL2749645T3/pl unknown
- 2012-08-21 EP EP17153617.0A patent/EP3192867A1/en not_active Withdrawn
- 2012-08-21 HR HRP20170813TT patent/HRP20170813T1/hr unknown
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101189332B (zh) * | 2005-06-01 | 2011-02-16 | Cj第一制糖株式会社 | 利用d-阿洛酮糖差向异构酶的d-阿洛酮糖生产方法 |
Non-Patent Citations (1)
| Title |
|---|
| Improvement in the Thermostability of D-Psicose 3-Epimerase from Agrobacterium tumefaciens by Random and Site-Directed Mutagenesis;Jin-Geun Choi 等;《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》;20111031;第77卷(第20期);7316-7320 * |
Also Published As
| Publication number | Publication date |
|---|---|
| KR101203856B1 (ko) | 2012-11-21 |
| IN2014CN01614A (https=) | 2015-05-08 |
| ES2626501T3 (es) | 2017-07-25 |
| US9217166B2 (en) | 2015-12-22 |
| SI2749645T1 (sl) | 2017-08-31 |
| CN104160023A (zh) | 2014-11-19 |
| RS56088B1 (sr) | 2017-10-31 |
| WO2013027999A2 (ko) | 2013-02-28 |
| US20140199732A1 (en) | 2014-07-17 |
| EP2749645B1 (en) | 2017-04-26 |
| JP6043354B2 (ja) | 2016-12-14 |
| EP3192867A1 (en) | 2017-07-19 |
| HRP20170813T1 (hr) | 2017-08-11 |
| PL2749645T3 (pl) | 2017-09-29 |
| WO2013027999A9 (ko) | 2013-07-11 |
| EP2749645A2 (en) | 2014-07-02 |
| EP2749645A4 (en) | 2015-06-10 |
| WO2013027999A3 (ko) | 2013-05-10 |
| PT2749645T (pt) | 2017-07-28 |
| JP2014525244A (ja) | 2014-09-29 |
| DK2749645T3 (en) | 2017-08-21 |
| HUE035316T2 (en) | 2018-05-02 |
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