IN2014CN01614A - - Google Patents

Info

Publication number
IN2014CN01614A
IN2014CN01614A IN1614CHN2014A IN2014CN01614A IN 2014CN01614 A IN2014CN01614 A IN 2014CN01614A IN 1614CHN2014 A IN1614CHN2014 A IN 1614CHN2014A IN 2014CN01614 A IN2014CN01614 A IN 2014CN01614A
Authority
IN
India
Prior art keywords
psicose
relates
present
recombinant vector
mutant
Prior art date
Application number
Inventor
Yang Hee Kim
Jin Ha Kim
Young Mi Lee
Young Ho Hong
Min Hae Kim
Seong Bo Kim
Seung Won Park
Seung Hyun Oh
Deok Kun Oh
Jin Geun Choi
Original Assignee
Cj Cheiljedang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cj Cheiljedang Corp filed Critical Cj Cheiljedang Corp
Publication of IN2014CN01614A publication Critical patent/IN2014CN01614A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/24—Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y501/00—Racemaces and epimerases (5.1)
    • C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)

Abstract

The present invention relates to a D psicose 3 epimerase mutant of which the thermal stability is improved by substituting an amino acid of a specific sequence number. In addition the present invention relates to a recombinant vector comprising the gene of the D psicose 3 epimerase mutant and a recombinant strain transformed with the recombinant vector. Further the present invention relates to an immobilized reactor prepared using the enzyme mutant or the recombinant vector and a method for producing D psicose using the immobilized reactor.
IN1614CHN2014 2011-08-24 2012-08-21 IN2014CN01614A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020110084712A KR101203856B1 (en) 2011-08-24 2011-08-24 D-psicose 3-epimerase variants improved thermostability and continuous production of D-psicose using the variants
PCT/KR2012/006637 WO2013027999A2 (en) 2011-08-24 2012-08-21 D-psicose 3-epimerase mutant with improved thermal stability, and continuous production of d-psicose using same

Publications (1)

Publication Number Publication Date
IN2014CN01614A true IN2014CN01614A (en) 2015-05-08

Family

ID=47565145

Family Applications (1)

Application Number Title Priority Date Filing Date
IN1614CHN2014 IN2014CN01614A (en) 2011-08-24 2012-08-21

Country Status (15)

Country Link
US (1) US9217166B2 (en)
EP (2) EP3192867A1 (en)
JP (1) JP6043354B2 (en)
KR (1) KR101203856B1 (en)
CN (1) CN104160023B (en)
DK (1) DK2749645T3 (en)
ES (1) ES2626501T3 (en)
HR (1) HRP20170813T1 (en)
HU (1) HUE035316T2 (en)
IN (1) IN2014CN01614A (en)
PL (1) PL2749645T3 (en)
PT (1) PT2749645T (en)
RS (1) RS56088B1 (en)
SI (1) SI2749645T1 (en)
WO (1) WO2013027999A2 (en)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101318422B1 (en) * 2013-04-09 2013-10-15 주식회사 삼양제넥스 D-psicose epimerase and method for producing psicose using the same
KR101455624B1 (en) 2013-04-12 2014-10-28 주식회사한국야쿠르트 Novel D-psicose-3-epimerase from Clostridium bolteae having production of functional rare sugar D-psicose and production method of D-psicose using thereof
KR101455759B1 (en) * 2013-04-23 2014-10-28 씨제이제일제당(주) D-psicose 3-epimerase variants and production method of D-psicose using them
EP2843044A1 (en) * 2013-09-03 2015-03-04 Roquette Frères Improved variant of D-psicose 3-epimerase and uses thereof
CN105637089B (en) * 2013-09-03 2021-06-15 罗盖特兄弟公司 Improved variants of D-psicose 3-epimerase and uses thereof
KR101539097B1 (en) 2013-12-26 2015-07-23 주식회사 삼양제넥스 Polynucleotide encoding psicose epimerase and method of producing psicos using the same
KR101473918B1 (en) 2014-05-28 2014-12-17 대상 주식회사 D-psicose 3-epimerase, manufacturing method thereof and manufacturing method of D-psicose using the same
KR101577147B1 (en) * 2014-10-01 2015-12-11 경상대학교산학협력단 Preparing method for psicose
KR101607633B1 (en) 2014-10-21 2016-04-11 주식회사 삼양사 Method for producing psicose using the corynebacterium
CN107257856B (en) * 2014-10-30 2021-05-28 株式会社三养社 Expression system of psicose epimerase and production of psicose using the same
US10266862B2 (en) 2014-11-06 2019-04-23 Industry-Academic Cooperation Foundation Gyeongsang National University Method for preparing psicose
KR102044957B1 (en) * 2014-12-31 2019-11-14 주식회사 삼양사 Method for producing psicose using the same
KR101677368B1 (en) * 2015-04-02 2016-11-18 씨제이제일제당 (주) A novel promoter and use thereof
KR102087396B1 (en) * 2015-11-16 2020-03-10 주식회사 삼양사 Method of preparing psicose from fructose-containing substrate
KR101807507B1 (en) 2015-12-23 2017-12-12 씨제이제일제당(주) Composition for preparing D-psicose comprising D-psicose 3-epimerase and salt, and method for preparing D-psicose using the same
KR101723007B1 (en) 2016-02-29 2017-04-04 씨제이제일제당(주) A method of manufacturing high purity d-psicose
CN105821027B (en) * 2016-04-01 2023-11-21 南京朗奈生物技术有限公司 Application of 3-epimerase
KR101905468B1 (en) * 2016-07-29 2018-10-10 씨제이제일제당 주식회사 Hexuronate c4-epimerase variants with improved conversion activity to d-tagatose and method for production of d-tagatose using them
CN106148311B (en) * 2016-09-12 2019-06-21 上海立足生物科技有限公司 A kind of mutant of D-Psicose -3- epimerase and its application
WO2018093153A1 (en) * 2016-11-16 2018-05-24 씨제이제일제당(주) Novel d-psicose 3-epimerase and method for preparing d-psicose using same
CN108239633B (en) * 2016-12-26 2023-05-05 上海立足生物科技有限公司 Mutant of D-psicose-3-epimerase with improved catalytic activity and application thereof
CN110300800A (en) 2017-01-06 2019-10-01 绿光生物科技股份有限公司 The cell-free production of sugar
MX2020007513A (en) * 2018-01-24 2021-03-18 Matsutani Kagaku Kogyo Kk Ketose 3-epimerase with improved thermal stability.
KR102439295B1 (en) 2018-11-30 2022-09-02 씨제이제일제당 주식회사 D-psicose crystal and a preparation method thereof
CN110396513B (en) * 2019-07-19 2022-01-11 天津科技大学 Mutant of D-psicose-3-epimerase and application thereof
CN110684762B (en) * 2019-11-12 2021-03-19 南京工业大学 D-psicose-3-epimerase mutant and encoding gene, recombinant vector, recombinant strain and application thereof
CN111019928B (en) * 2019-12-11 2022-08-16 吉林中粮生化有限公司 Coding gene, vector and recombinant cell of D-psicose 3-epimerase and application thereof
KR102254411B1 (en) 2019-12-19 2021-05-24 대상 주식회사 Variant of D-allulose 3-epimerase, manufacturing method thereof and manufacturing method of D-alluose using the same
WO2021199445A1 (en) * 2020-04-03 2021-10-07 Rensselaer Polytechnic Institute Method for producing sulfated polysaccharide and method for producing paps
KR102448351B1 (en) 2020-04-27 2022-09-28 대상 주식회사 Variant of D-allulose 3-epimerase, manufacturing method thereof and manufacturing method of D-alluose using the same
WO2021244005A1 (en) 2020-06-03 2021-12-09 中国科学院天津工业生物技术研究所 Allulose 3-epimerase mutant, engineered bacterium expressing same, and immobilized enzyme and immobilization method thereof
CN111793616B (en) * 2020-08-07 2022-04-12 天津科技大学 Epimerase mutant and application thereof
KR102682846B1 (en) 2021-11-19 2024-07-08 대상 주식회사 Variant of D-allulose 3-epimerase with excellent heat stability, manufacturing method thereof and manufacturing method of D-alluose using the same
KR20240108871A (en) * 2022-12-30 2024-07-10 주식회사 삼양사 Mutant allulose-3-epimerase and use thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100744479B1 (en) 2005-06-01 2007-08-01 씨제이 주식회사 D-Psicose production method by D-psicose epimerase
GB0512150D0 (en) * 2005-06-15 2005-07-20 Scottish Crop Res Inst Production of L-ascorbic acid
KR20110035805A (en) * 2009-09-30 2011-04-06 씨제이제일제당 (주) Method of producing d-psicose using immobilized d-psicose 3-epimerase

Also Published As

Publication number Publication date
JP2014525244A (en) 2014-09-29
EP3192867A1 (en) 2017-07-19
CN104160023A (en) 2014-11-19
KR101203856B1 (en) 2012-11-21
HRP20170813T1 (en) 2017-08-11
ES2626501T3 (en) 2017-07-25
PL2749645T3 (en) 2017-09-29
WO2013027999A2 (en) 2013-02-28
CN104160023B (en) 2017-03-29
EP2749645B1 (en) 2017-04-26
EP2749645A4 (en) 2015-06-10
HUE035316T2 (en) 2018-05-02
WO2013027999A9 (en) 2013-07-11
PT2749645T (en) 2017-07-28
RS56088B1 (en) 2017-10-31
SI2749645T1 (en) 2017-08-31
WO2013027999A3 (en) 2013-05-10
US9217166B2 (en) 2015-12-22
EP2749645A2 (en) 2014-07-02
DK2749645T3 (en) 2017-08-21
JP6043354B2 (en) 2016-12-14
US20140199732A1 (en) 2014-07-17

Similar Documents

Publication Publication Date Title
IN2014CN01614A (en)
NZ602958A (en) High level expression of recombinant toxin proteins
BR112012016318A2 (en) "Isolated alpha amylase, composition, method for producing liquefied starch, process for synthesizing a fermentation product, nucleic acid sequence, host cell, and method for preparing an alpha amylase."
IN2014DN03419A (en)
WO2010006312A3 (en) Methods and compositions for producing carbon-based products in microorganisms
EA201001435A1 (en) CELL, ENZYME SUGAR-PENTOSE
IN2014CN02163A (en)
MX343755B (en) O-phosphoserine sulfhydrylase mutants and method for production of cysteine using the same.
MY193665A (en) Recombinant microorganisms and uses therefor
IN2014CN01706A (en)
MY170507A (en) A microorganism having enhanced l-amino acids productivity and process for producing l-amino acids using the same
WO2009125924A3 (en) Mutant microorganism with high ability of producing putrescine and preparation of putrescine using same
NZ766918A (en) Clostridium histolyticum enzymes and methods for the use thereof
IN2014DN00094A (en)
MY164858A (en) Microorganisms of corynebacterium which can utilize xylose and method for producing l-lysine using same
MX2013005853A (en) Fusion enzymes having n-acetylglucosaminyltransferase activity.
CA2863578C (en) Methods and compositions for increasing sialic acid production and treating sialic related disease conditions
EA201001436A1 (en) CELL CONTAINING PENTOUS SUGAR
IN2014MN00854A (en)
MX355134B (en) Method for producing high yield attenuated salmonella strains.
WO2010101360A3 (en) Microorganism which produces l-amino acid and method for producing l-amino acid using the same
WO2013085361A3 (en) Mutant microorganism having a high 4-hydroxybutyric acid production capacity, and method for preparing 4-hydroxybutyric acid using same
MY170467A (en) High molecular weight pha-producing microbe and method of producing high molecular weight pha using same
WO2012087038A3 (en) Novel o-acetylhomoserine sulfhydrylase or variant thereof, and method for transforming methionine using same
MY157302A (en) A genetically engineered bacterial strain wsj-ia i producing high content and high yield of isovaleryl spiramycin