WO2013085361A3 - Mutant microorganism having a high 4-hydroxybutyric acid production capacity, and method for preparing 4-hydroxybutyric acid using same - Google Patents

Mutant microorganism having a high 4-hydroxybutyric acid production capacity, and method for preparing 4-hydroxybutyric acid using same Download PDF

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Publication number
WO2013085361A3
WO2013085361A3 PCT/KR2012/010665 KR2012010665W WO2013085361A3 WO 2013085361 A3 WO2013085361 A3 WO 2013085361A3 KR 2012010665 W KR2012010665 W KR 2012010665W WO 2013085361 A3 WO2013085361 A3 WO 2013085361A3
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WO
WIPO (PCT)
Prior art keywords
hydroxybutyric acid
mutant microorganism
production capacity
preparing
acid production
Prior art date
Application number
PCT/KR2012/010665
Other languages
French (fr)
Korean (ko)
Other versions
WO2013085361A2 (en
Inventor
이상엽
최솔
이정욱
박시재
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한국과학기술원
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Publication of WO2013085361A2 publication Critical patent/WO2013085361A2/en
Publication of WO2013085361A3 publication Critical patent/WO2013085361A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/52—Propionic acid; Butyric acids

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a mutant microorganism having a high 4-hydroxybutyric acid production capacity and to a method for preparing 4-hydroxybutyric acid using the mutant microorganism. More particularly, the present invention relates to a mutant microorganism having a high 4-hydroxybutyric acid production capacity, in which genes for synthesizing 4-hydroxybutyric acid from succinic acid are amplified, genes in an anaplerotic pathway are amplified, and competitive genes are deleted so as to increase the 4-hydroxybutyric acid production capacity. The present invention also relates to a method for preparing 4-hydroxybutyric acid with a high yield rate by culturing the mutant microorganism under microaerobic conditions. The present invention enables the preparation of a mutant microorganism having a high 4-hydroxybutyric acid production capacity and the use of the mutant microorganism, and therefore is useful in the preparation of 4-hydroxybutyric acid with a high yield rate.
PCT/KR2012/010665 2011-12-07 2012-12-07 Mutant microorganism having a high 4-hydroxybutyric acid production capacity, and method for preparing 4-hydroxybutyric acid using same WO2013085361A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2011-0130183 2011-12-07
KR20110130183 2011-12-07

Publications (2)

Publication Number Publication Date
WO2013085361A2 WO2013085361A2 (en) 2013-06-13
WO2013085361A3 true WO2013085361A3 (en) 2013-10-17

Family

ID=48575027

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2012/010665 WO2013085361A2 (en) 2011-12-07 2012-12-07 Mutant microorganism having a high 4-hydroxybutyric acid production capacity, and method for preparing 4-hydroxybutyric acid using same

Country Status (2)

Country Link
KR (1) KR101587618B1 (en)
WO (1) WO2013085361A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102781901B (en) 2010-02-11 2016-08-03 梅塔玻利克斯公司 For the method preparing monomer component from genetically modified polyhydroxyalkanoatefrom biomass
KR102097065B1 (en) * 2013-08-23 2020-04-03 삼성전자주식회사 A microorganism producing 4-hydroxybutyrate and a method for producing 4-hydroxybutyrate in anaerobic condition using the same
EP3214176B1 (en) 2014-10-30 2020-04-01 Samyang Corporation Expression system of psicose epimerase and production of psicose using same
CN108588105B (en) * 2018-05-03 2022-02-01 成都理工大学 Escherichia coli expression vector, construction method and application thereof
KR102120995B1 (en) * 2018-10-12 2020-06-18 경희대학교 산학협력단 Transformed methanotrophs for producing 4-Hydroxybutyric acid and uses thereof
KR102105689B1 (en) * 2018-12-17 2020-04-28 울산과학기술원 Lactate dehydrogenase mutants and preparing method of 2-hydroxy-carboxylic acid
KR102339122B1 (en) * 2020-03-11 2021-12-14 경희대학교 산학협력단 Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) from methane by metabolic engineered methanotrophs

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010006076A2 (en) * 2008-07-08 2010-01-14 Opx Biotechnologies Inc. Methods, compositions and systems for biosynthetic bio production of 1,4-butanediol
KR100957772B1 (en) * 2006-03-23 2010-05-12 주식회사 엘지화학 Mutants Having a Producing Ability of 4?hydroxybutyrate and Method for Preparing 4HB Using the Same
WO2010141920A2 (en) * 2009-06-04 2010-12-09 Genomatica, Inc. Microorganisms for the production of 1,4-butanediol and related methods
KR101042242B1 (en) * 2007-09-07 2011-06-17 한국과학기술원 Mutants having a producing ability of 1,4-butanediol and method for preparing 1,4-butanediol using the same

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100957772B1 (en) * 2006-03-23 2010-05-12 주식회사 엘지화학 Mutants Having a Producing Ability of 4?hydroxybutyrate and Method for Preparing 4HB Using the Same
KR101042242B1 (en) * 2007-09-07 2011-06-17 한국과학기술원 Mutants having a producing ability of 1,4-butanediol and method for preparing 1,4-butanediol using the same
WO2010006076A2 (en) * 2008-07-08 2010-01-14 Opx Biotechnologies Inc. Methods, compositions and systems for biosynthetic bio production of 1,4-butanediol
WO2010141920A2 (en) * 2009-06-04 2010-12-09 Genomatica, Inc. Microorganisms for the production of 1,4-butanediol and related methods

Also Published As

Publication number Publication date
KR20130064037A (en) 2013-06-17
WO2013085361A2 (en) 2013-06-13
KR101587618B1 (en) 2016-01-22

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