WO2012119546A3 - Method for preparing recombinant escherichia coli to produce succinic acid through fermentation - Google Patents

Method for preparing recombinant escherichia coli to produce succinic acid through fermentation Download PDF

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Publication number
WO2012119546A3
WO2012119546A3 PCT/CN2012/072012 CN2012072012W WO2012119546A3 WO 2012119546 A3 WO2012119546 A3 WO 2012119546A3 CN 2012072012 W CN2012072012 W CN 2012072012W WO 2012119546 A3 WO2012119546 A3 WO 2012119546A3
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WO
WIPO (PCT)
Prior art keywords
succinic acid
escherichia coli
fermentation
recombinant escherichia
preparing recombinant
Prior art date
Application number
PCT/CN2012/072012
Other languages
French (fr)
Chinese (zh)
Other versions
WO2012119546A2 (en
Inventor
姜岷
马江锋
陈可泉
郭亭
韦萍
欧阳平凯
Original Assignee
南京工业大学
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Publication date
Application filed by 南京工业大学 filed Critical 南京工业大学
Publication of WO2012119546A2 publication Critical patent/WO2012119546A2/en
Publication of WO2012119546A3 publication Critical patent/WO2012119546A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1077—Pentosyltransferases (2.4.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A method for preparing recombinant Escherichia coli to produce succinic acid through fermentation. The method mainly involves increasing the synthesis rate of succinic acid by overexpressing NAD+ biosynthesis-related genes in Escherichia coli, and adding a certain concentration of nicotinic acid to the anaerobic culture medium as the precursor material of NAD+ biosynthesis, thus increasing the strain growth rate in anaerobic conditions and the synthesis rate of succinic acid.
PCT/CN2012/072012 2011-03-09 2012-03-06 Method for preparing recombinant escherichia coli to produce succinic acid through fermentation WO2012119546A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN2011100550421A CN102174458A (en) 2011-03-09 2011-03-09 Method for preparing recombinant escherichia coli to produce succinic acid through fermentation
CN2011100550421 2011-03-09

Publications (2)

Publication Number Publication Date
WO2012119546A2 WO2012119546A2 (en) 2012-09-13
WO2012119546A3 true WO2012119546A3 (en) 2012-11-01

Family

ID=44517716

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2012/072012 WO2012119546A2 (en) 2011-03-09 2012-03-06 Method for preparing recombinant escherichia coli to produce succinic acid through fermentation

Country Status (2)

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CN (1) CN102174458A (en)
WO (1) WO2012119546A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102174458A (en) * 2011-03-09 2011-09-07 南京工业大学 Method for preparing recombinant escherichia coli to produce succinic acid through fermentation
CN103361384B (en) * 2012-04-10 2016-04-13 中国石油化工股份有限公司 A kind of fermentation process adding carboxylated Factor and prepare succinic acid
CN106635943B (en) * 2016-12-19 2019-09-03 江南大学 A method of improving oxidized coenzyme I content intracellular

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1202930A (en) * 1995-11-02 1998-12-23 芝加哥大学 A mutant E. coli strain with increased succinic acid production
CN101044245A (en) * 2004-08-27 2007-09-26 莱斯大学 Mutant e. coli strain with increased succinic acid production
CN102154339A (en) * 2011-02-16 2011-08-17 南京工业大学 Construction method of gene engineering strain for producing succinic acid escherichia coli
CN102174458A (en) * 2011-03-09 2011-09-07 南京工业大学 Method for preparing recombinant escherichia coli to produce succinic acid through fermentation

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1202930A (en) * 1995-11-02 1998-12-23 芝加哥大学 A mutant E. coli strain with increased succinic acid production
CN101044245A (en) * 2004-08-27 2007-09-26 莱斯大学 Mutant e. coli strain with increased succinic acid production
CN102154339A (en) * 2011-02-16 2011-08-17 南京工业大学 Construction method of gene engineering strain for producing succinic acid escherichia coli
CN102174458A (en) * 2011-03-09 2011-09-07 南京工业大学 Method for preparing recombinant escherichia coli to produce succinic acid through fermentation

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
JIANG, MIN ET AL.: "The Progress of Recombinant Escherichia coli for Production of Succinic Acid", MICROBIOLOGY, vol. 36, no. 1, 20 January 2009 (2009-01-20), pages 120 - 124 *
LIU, RONGMING ET AL.: "Effect of overexpression of nicotinic acid phosphoribosyl transferase on succinic acid production in Escherichia coli NZN111", CHINESE JOURNAL OF BIOTECHNOLOGY, 25 October 2011 (2011-10-25), pages 1438 - 1447 *
QIN, YI ET AL.: "Manipulation of NADH metabolism in industrial strains", CHINESE JOURNAL OF BIOTECHNOLOGY, vol. 25, no. 2, 25 February 2009 (2009-02-25), pages 161 - 169 *
VEMURI, G.N. ET AL.: "Effects of Growth Mode and Pyruvate Carboxylase on Succinic Acid Production by Metabolically Engineered Strains of Escherichia coli", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 68, no. 4, 28 April 2002 (2002-04-28), pages 1715 - 1727 *

Also Published As

Publication number Publication date
WO2012119546A2 (en) 2012-09-13
CN102174458A (en) 2011-09-07

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