IN2014DN03419A - - Google Patents
Info
- Publication number
- IN2014DN03419A IN2014DN03419A IN3419DEN2014A IN2014DN03419A IN 2014DN03419 A IN2014DN03419 A IN 2014DN03419A IN 3419DEN2014 A IN3419DEN2014 A IN 3419DEN2014A IN 2014DN03419 A IN2014DN03419 A IN 2014DN03419A
- Authority
- IN
- India
- Prior art keywords
- promoter
- poi
- cell line
- cultivating
- repressing
- Prior art date
Links
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 2
- 229910052799 carbon Inorganic materials 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 abstract 2
- 230000000754 repressing effect Effects 0.000 abstract 2
- 238000012258 culturing Methods 0.000 abstract 1
- 210000003527 eukaryotic cell Anatomy 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 abstract 1
- 230000000153 supplemental effect Effects 0.000 abstract 1
- 238000013518 transcription Methods 0.000 abstract 1
- 230000035897 transcription Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
- C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
- C12Y102/01012—Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) (1.2.1.12)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/55—Fab or Fab'
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter b) cultivating the cell line with a limited amount of a supplemental carbon source de repressing the promoter to induce production of the POI at a transcription rate of at least 15% as compared to the native pGAP promoter and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161544451P | 2011-10-07 | 2011-10-07 | |
| EP11184323 | 2011-10-07 | ||
| EP12171006 | 2012-06-06 | ||
| PCT/EP2012/069757 WO2013050551A1 (en) | 2011-10-07 | 2012-10-05 | Regulatable promoter |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IN2014DN03419A true IN2014DN03419A (en) | 2015-05-22 |
Family
ID=48043194
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IN3419DEN2014 IN2014DN03419A (en) | 2011-10-07 | 2012-10-05 |
Country Status (17)
| Country | Link |
|---|---|
| US (4) | US9512432B2 (en) |
| EP (2) | EP2764016B1 (en) |
| JP (1) | JP6113736B2 (en) |
| KR (2) | KR102128957B1 (en) |
| CN (3) | CN103998460A (en) |
| AR (1) | AR088252A1 (en) |
| AU (1) | AU2012320421B2 (en) |
| BR (1) | BR112014008152A2 (en) |
| CA (1) | CA2849856C (en) |
| DK (1) | DK3508494T3 (en) |
| ES (1) | ES2913825T3 (en) |
| IL (1) | IL231859B (en) |
| IN (1) | IN2014DN03419A (en) |
| PL (1) | PL2764016T3 (en) |
| SG (2) | SG11201400811QA (en) |
| TW (1) | TWI560272B (en) |
| WO (1) | WO2013050551A1 (en) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IN2014DN03419A (en) * | 2011-10-07 | 2015-05-22 | Lonza Ag | |
| CN104195165A (en) * | 2014-09-01 | 2014-12-10 | 北京爱必信生物技术有限公司 | Vector comprising glucose promoter, host bacterium and preparation method of recombinant bovine-derived trypsinase |
| US11518797B2 (en) | 2014-11-11 | 2022-12-06 | Clara Foods Co. | Methods and compositions for egg white protein production |
| JP6833812B2 (en) | 2015-08-05 | 2021-02-24 | ロンザ リミテッドＬｏｎｚａ Ｌｉｍｉｔｅｄ | Promoter mutant |
| CN109219662A (en) | 2016-03-02 | 2019-01-15 | 龙沙有限公司 | Improved fermentation process |
| CN110088279A (en) * | 2016-12-15 | 2019-08-02 | 株式会社钟化 | Novel host cell and used its target protein manufacturing method |
| WO2019154686A1 (en) | 2018-02-12 | 2019-08-15 | Lonza Ltd | Host cell for producing a protein of interest |
| EP3536784A1 (en) | 2018-03-05 | 2019-09-11 | ACIB GmbH | Host cell engineered for improved metabolite production |
| WO2019236451A1 (en) * | 2018-06-07 | 2019-12-12 | Basf Se | Promoter for yeast |
| KR102495283B1 (en) * | 2019-01-11 | 2023-02-06 | 론자 리미티드 | Carbon Source Controlled Protein Production in Recombinant Host Cells |
| HUE062297T2 (en) * | 2019-01-29 | 2023-10-28 | Clariant Produkte Deutschland Gmbh | Chimeric promoter for use in metabolic engineering |
| WO2020200415A1 (en) | 2019-04-01 | 2020-10-08 | Lonza Ltd | Mut- methylotrophic yeast |
| WO2020200414A1 (en) | 2019-04-01 | 2020-10-08 | Lonza Ltd | Protein production in mut-methylotrophic yeast |
| AU2020252241B2 (en) | 2019-04-01 | 2024-03-21 | Universität Für Bodenkultur Wien | Mut- methylotrophic yeast |
| US12096784B2 (en) | 2019-07-11 | 2024-09-24 | Clara Foods Co. | Protein compositions and consumable products thereof |
| BR112022000545A2 (en) | 2019-07-11 | 2022-04-05 | Clara Foods Co | Protein compositions and consumer products of these |
| US10927360B1 (en) | 2019-08-07 | 2021-02-23 | Clara Foods Co. | Compositions comprising digestive enzymes |
| US20230374526A1 (en) | 2020-03-26 | 2023-11-23 | Asklepios Biopharmaceutical, Inc. | Forskolin-inducible promoters and hypoxia-inducible promoters |
| EP4127185B1 (en) | 2020-04-01 | 2025-06-04 | Lonza Ltd. | Helper factors for expressing proteins in yeast |
| EP4136237A1 (en) | 2020-04-15 | 2023-02-22 | Asklepios Biopharmaceutical, Inc. | Forskolin-inducible promoters and hypoxia-inducible promoters |
| KR20230075436A (en) | 2020-09-30 | 2023-05-31 | 론자 리미티드 | Host cells overexpressing translation factors |
| WO2022171827A1 (en) | 2021-02-12 | 2022-08-18 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Signal peptides for increased protein secretion |
| WO2024133538A1 (en) | 2022-12-20 | 2024-06-27 | Lonza Ltd | Host cells with pat1 knockout for increased specific protein productivity |
| WO2025247966A1 (en) | 2024-05-29 | 2025-12-04 | Mybios Gmbh | Process for protein production in methylotrophic yeast host cell. |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU577259B2 (en) | 1982-08-13 | 1988-09-22 | Zymogenetics Inc. | Glycolytic promters for regulated protein expression protease inhibitor |
| US4808537A (en) | 1984-10-30 | 1989-02-28 | Phillips Petroleum Company | Methanol inducible genes obtained from pichia and methods of use |
| US4855231A (en) | 1984-10-30 | 1989-08-08 | Phillips Petroleum Company | Regulatory region for heterologous gene expression in yeast |
| AU2887897A (en) | 1996-05-21 | 1997-12-09 | Novo Nordisk A/S | Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast |
| SE9802333D0 (en) | 1998-06-29 | 1998-06-29 | Astra Pharma Prod | Novel combination |
| US6730499B1 (en) | 1998-07-03 | 2004-05-04 | Research Corporation Technologies, Inc. | Promoter for the Pichia pastoris formaldehyde dehydrogenase gene FLD1 |
| US7259255B2 (en) | 2003-06-25 | 2007-08-21 | E. I. Du Pont De Nemours And Company | Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast |
| WO2006024972A2 (en) | 2004-07-22 | 2006-03-09 | Glycofi, Inc | Malate synthase regulatory sequences for heterologous gene expression in pichia |
| AT501955B1 (en) | 2005-02-23 | 2007-08-15 | Univ Graz Tech | MUTED AOX1 PROMOTERS |
| ATE486944T1 (en) | 2005-11-16 | 2010-11-15 | Korea Res Inst Of Bioscience | TRANSLATION SELONGATION FACTOR PROMOTER FROM PICHIA PASTORIS AND METHOD FOR PRODUCING RECOMBINANT PROTEIN USING THEREOF |
| KR100717353B1 (en) | 2006-04-12 | 2007-05-11 | 한국생명공학연구원 | Auto-inducible sodium phosphate symporter promoter from pichia pastoris and method for producing recombinant protein using it |
| CN100552029C (en) | 2006-09-14 | 2009-10-21 | 南开大学 | Pichia pastoris ω3-fatty acid dehydrogenase promoter sequence and its application |
| WO2008063302A2 (en) | 2006-10-10 | 2008-05-29 | Keck Graduate Institute | Novel p. pastoris pastoris promoters, and the use thereof to direct expression of proteins in yeast, preferably using a haploid mating strategy |
| CA2684650A1 (en) | 2007-04-20 | 2008-10-30 | Polymun Scientific Immunbiologische Forschung Gmbh | Expression system |
| US8105811B2 (en) | 2008-06-13 | 2012-01-31 | Wisconsin Alumni Research Foundation | Sugar transport sequences, yeast strains having improved sugar uptake, and methods of use |
| US8440456B2 (en) * | 2009-05-22 | 2013-05-14 | Vib, Vzw | Nucleic acids of Pichia pastoris and use thereof for recombinant production of proteins |
| BRPI1011934A8 (en) * | 2009-07-09 | 2017-10-03 | Verdezyne Inc | COMPOSITION |
| EP2292769A1 (en) * | 2009-08-10 | 2011-03-09 | Lonza AG | Fermentation process |
| IN2014DN03419A (en) * | 2011-10-07 | 2015-05-22 | Lonza Ag | |
| JP6833812B2 (en) | 2015-08-05 | 2021-02-24 | ロンザ リミテッドＬｏｎｚａ Ｌｉｍｉｔｅｄ | Promoter mutant |
-
2012
- 2012-10-05 IN IN3419DEN2014 patent/IN2014DN03419A/en unknown
- 2012-10-05 DK DK19156035.8T patent/DK3508494T3/en active
- 2012-10-05 SG SG11201400811QA patent/SG11201400811QA/en unknown
- 2012-10-05 BR BR112014008152A patent/BR112014008152A2/en not_active Application Discontinuation
- 2012-10-05 AU AU2012320421A patent/AU2012320421B2/en not_active Ceased
- 2012-10-05 AR ARP120103723A patent/AR088252A1/en not_active Application Discontinuation
- 2012-10-05 JP JP2014533924A patent/JP6113736B2/en active Active
- 2012-10-05 CN CN201280060753.0A patent/CN103998460A/en active Pending
- 2012-10-05 KR KR1020197005781A patent/KR102128957B1/en active Active
- 2012-10-05 PL PL12768859T patent/PL2764016T3/en unknown
- 2012-10-05 WO PCT/EP2012/069757 patent/WO2013050551A1/en not_active Ceased
- 2012-10-05 EP EP12768859.6A patent/EP2764016B1/en active Active
- 2012-10-05 CN CN201910140886.2A patent/CN110055248A/en active Pending
- 2012-10-05 CN CN202110953383.4A patent/CN113788884B/en active Active
- 2012-10-05 CA CA2849856A patent/CA2849856C/en active Active
- 2012-10-05 ES ES19156035T patent/ES2913825T3/en active Active
- 2012-10-05 KR KR1020147009284A patent/KR101954389B1/en active Active
- 2012-10-05 SG SG10201602673PA patent/SG10201602673PA/en unknown
- 2012-10-05 EP EP19156035.8A patent/EP3508494B1/en active Active
- 2012-10-05 US US14/350,352 patent/US9512432B2/en active Active
- 2012-10-05 TW TW101137171A patent/TWI560272B/en not_active IP Right Cessation
-
2014
- 2014-04-01 IL IL231859A patent/IL231859B/en active IP Right Grant
-
2016
- 2016-10-26 US US15/335,132 patent/US10301634B2/en active Active
-
2019
- 2019-03-05 US US16/293,479 patent/US11401523B2/en active Active
-
2022
- 2022-03-18 US US17/698,813 patent/US11976284B2/en active Active
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