HRP20170813T1 - Mutant d-psikoza-3-epimeraze s povećanom toplinskom stabilnošću i kontinuirana proizvodnja d-psikoze uz upotrebu iste - Google Patents
Mutant d-psikoza-3-epimeraze s povećanom toplinskom stabilnošću i kontinuirana proizvodnja d-psikoze uz upotrebu iste Download PDFInfo
- Publication number
- HRP20170813T1 HRP20170813T1 HRP20170813TT HRP20170813T HRP20170813T1 HR P20170813 T1 HRP20170813 T1 HR P20170813T1 HR P20170813T T HRP20170813T T HR P20170813TT HR P20170813 T HRP20170813 T HR P20170813T HR P20170813 T1 HRP20170813 T1 HR P20170813T1
- Authority
- HR
- Croatia
- Prior art keywords
- psicose
- amino acid
- variant
- acid sequence
- epimerase
- Prior art date
Links
- 108030002106 D-psicose 3-epimerases Proteins 0.000 title claims 4
- BJHIKXHVCXFQLS-PUFIMZNGSA-N D-psicose Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)C(=O)CO BJHIKXHVCXFQLS-PUFIMZNGSA-N 0.000 title claims 3
- 238000010924 continuous production Methods 0.000 title 1
- 150000001413 amino acids Chemical group 0.000 claims 9
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims 4
- 208000028017 Psychotic disease Diseases 0.000 claims 4
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 claims 4
- 235000018417 cysteine Nutrition 0.000 claims 4
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims 4
- 238000004519 manufacturing process Methods 0.000 claims 4
- 229930091371 Fructose Natural products 0.000 claims 3
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims 3
- 239000005715 Fructose Substances 0.000 claims 3
- 238000000034 method Methods 0.000 claims 3
- 241000186226 Corynebacterium glutamicum Species 0.000 claims 2
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 claims 2
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 claims 2
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 claims 2
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims 2
- 235000001014 amino acid Nutrition 0.000 claims 2
- 229940024606 amino acid Drugs 0.000 claims 2
- 239000013604 expression vector Substances 0.000 claims 2
- 239000012634 fragment Substances 0.000 claims 2
- 229960000310 isoleucine Drugs 0.000 claims 2
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 claims 2
- 238000003259 recombinant expression Methods 0.000 claims 2
- 239000004474 valine Substances 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
- C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/24—Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y501/00—Racemaces and epimerases (5.1)
- C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)
Claims (8)
1. Varijanta d-psikoza-3-epimeraze s povećanom toplinskom stabilnošću, naznačena time što varijanta ima aminokiselinski slijed sa SEQ ID NO:1 u Korejskoj patentnoj objavi br. 10-2011-0035805A ili funkcionalni fragment u kojem je izoleucin (Ile) na položaju 33 u aminokiselinskom slijedu zamijenjen aminokiselinom koja se bira iz skupine koju čine leucin (Leu), cistein (Cys) i valin (Val), ili aminokiselinski slijed u kojem je serin (Ser) na položaju 213 u aminokiselinskom slijedu zamijenjen cisteinom.
2. Varijanta d-psikoza-3-epimeraze s povećanom toplinskom stabilnošću, naznačena time što varijanta ima aminokiselinski slijed sa SEQ ID NO:1 u Korejskoj patentnoj objavi br. 10-2011-0035805A ili funkcionalni fragment u kojem je izoleucin (Ile) na položaju 33 u aminokiselinskom slijedu zamijenjen aminokiselinom koja se bira iz skupine koju čine leucin (Leu), cistein (Cys) i valin (Val), a serin (Ser) na položaju 213 u aminokiselinskom slijedu je zamijenjen cisteinom.
3. Rekombinantni ekspresijski vektor, naznačen time što sadrži gen koji kodira varijantu d-psikoza-3-epimeraze u skladu s patentnim zahtjevom 1 ili 2.
4. Bakterija Corynebacterium glutamicum pFIS-1-ATPE-2, naznačena time što je transformirana rekombinantnim ekspresijskim vektorom u skladu s patentnim zahtjevom 3.
5. Postupak proizvodnje d-psikoze iz fruktoze, naznačen time što se upotrebljava varijanta d-psikoza-3-epimeraze u skladu s patentnim zahtjevom 1 ili 2.
6. Postupak proizvodnje d-psikoze iz fruktoze, naznačen time što se upotrebljava rekombinantna bakterija Corynebacterium glutamicum pFIS-1-ATPE-2 u skladu s patentnim zahtjevom 4.
7. Imobilizirani reaktor za proizvodnju d-psikoze, naznačen time što sadrži kolonu ispunjenu nosačem na kojem je imobilizirana varijanta d-psikoza-3-epimeraze u skladu s patentnim zahtjevom 1 ili 2.
8. Postupak proizvodnje d-psikoze, naznačen time što se otopina fruktoze dodaje u imobilizirani reaktor u skladu s patentnim zahtjevom 7.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020110084712A KR101203856B1 (ko) | 2011-08-24 | 2011-08-24 | 열 안정성이 향상된 사이코스 에피머화 효소 변이체 및 이를 이용한 사이코스의 연속적 생산 |
| EP12826072.6A EP2749645B1 (en) | 2011-08-24 | 2012-08-21 | D-psicose 3-epimerase mutant with improved thermal stability, and continuous production of d-psicose using same |
| PCT/KR2012/006637 WO2013027999A2 (ko) | 2011-08-24 | 2012-08-21 | 열 안정성이 향상된 사이코스 에피머화 효소 변이체 및 이를 이용한 사이코스의 연속적 생산 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20170813T1 true HRP20170813T1 (hr) | 2017-08-11 |
Family
ID=47565145
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20170813TT HRP20170813T1 (hr) | 2011-08-24 | 2017-05-30 | Mutant d-psikoza-3-epimeraze s povećanom toplinskom stabilnošću i kontinuirana proizvodnja d-psikoze uz upotrebu iste |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US9217166B2 (hr) |
| EP (2) | EP3192867A1 (hr) |
| JP (1) | JP6043354B2 (hr) |
| KR (1) | KR101203856B1 (hr) |
| CN (1) | CN104160023B (hr) |
| DK (1) | DK2749645T3 (hr) |
| ES (1) | ES2626501T3 (hr) |
| HR (1) | HRP20170813T1 (hr) |
| HU (1) | HUE035316T2 (hr) |
| IN (1) | IN2014CN01614A (hr) |
| PL (1) | PL2749645T3 (hr) |
| PT (1) | PT2749645T (hr) |
| RS (1) | RS56088B1 (hr) |
| SI (1) | SI2749645T1 (hr) |
| WO (1) | WO2013027999A2 (hr) |
Families Citing this family (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101318422B1 (ko) * | 2013-04-09 | 2013-10-15 | 주식회사 삼양제넥스 | D-사이코스 에피머화 효소, 및 이를 이용하는 사이코스 생산방법 |
| KR101455624B1 (ko) | 2013-04-12 | 2014-10-28 | 주식회사한국야쿠르트 | 기능성 희귀당 사이코스의 생산능을 지닌 신규 클로스트리디움 볼티에 유래의 사이코스-3-에피머화 효소 및 이를 이용한 사이코스 생산방법 |
| KR101455759B1 (ko) | 2013-04-23 | 2014-10-28 | 씨제이제일제당(주) | 사이코스 에피머화 효소 변이체 및 이를 이용한 사이코스의 제조 방법 |
| EP2843044A1 (en) * | 2013-09-03 | 2015-03-04 | Roquette Frères | Improved variant of D-psicose 3-epimerase and uses thereof |
| JP6774875B2 (ja) * | 2013-09-03 | 2020-10-28 | ロケット フレールＲｏｑｕｅｔｔｅ Ｆｒｅｒｅｓ | Ｄ−プシコース ３−エピメラーゼの改良された変異体およびその使用 |
| KR101539097B1 (ko) | 2013-12-26 | 2015-07-23 | 주식회사 삼양제넥스 | 사이코스 에피머화 효소를 암호화 하는 폴리뉴클레오타이드, 및 이를 이용하는 사이코스 생산방법 |
| KR101473918B1 (ko) | 2014-05-28 | 2014-12-17 | 대상 주식회사 | 사이코스 에피머화 효소, 이의 제조방법 및 이를 이용한 사이코스의 제조방법 |
| KR101577147B1 (ko) * | 2014-10-01 | 2015-12-11 | 경상대학교산학협력단 | 사이코스의 생산 방법 |
| KR101607633B1 (ko) | 2014-10-21 | 2016-04-11 | 주식회사 삼양사 | 코리네박테리움속 균주를 이용한 사이코스 생산방법 |
| WO2016068656A2 (ko) | 2014-10-30 | 2016-05-06 | 주식회사 삼양사 | 사이코스 에퍼머화 효소의 발현 시스템 및 이를 이용한 사이코스의 생산 |
| US10266862B2 (en) | 2014-11-06 | 2019-04-23 | Industry-Academic Cooperation Foundation Gyeongsang National University | Method for preparing psicose |
| KR102044957B1 (ko) * | 2014-12-31 | 2019-11-14 | 주식회사 삼양사 | 사이코스의 제조방법 |
| KR101677368B1 (ko) | 2015-04-02 | 2016-11-18 | 씨제이제일제당 (주) | 신규 프로모터 및 이의 용도 |
| KR102087396B1 (ko) * | 2015-11-16 | 2020-03-10 | 주식회사 삼양사 | 과당-함유 기질로부터 사이코스를 생산하는 방법 |
| EP3395952B1 (en) * | 2015-12-23 | 2021-01-13 | Cj Cheiljedang Corporation | Composition for producing d-psicose comprising d-psicose 3-epimerase and salt and method for producing d-psicose using same |
| KR101723007B1 (ko) | 2016-02-29 | 2017-04-04 | 씨제이제일제당(주) | 고순도 d-사이코스를 제조하는 방법 |
| CN105821027B (zh) * | 2016-04-01 | 2023-11-21 | 南京朗奈生物技术有限公司 | 一种3-差向异构酶的用途 |
| KR101955103B1 (ko) * | 2016-07-29 | 2019-03-06 | 씨제이제일제당 (주) | D-타가토스 전환 활성이 향상된 헥수론산 c4-에피머화 효소 변이체 및 이를 이용한 d-타가토스의 제조 방법 |
| CN106148311B (zh) * | 2016-09-12 | 2019-06-21 | 上海立足生物科技有限公司 | 一种d-阿洛酮糖-3-差向异构酶的突变体及其应用 |
| UA126473C2 (uk) * | 2016-11-16 | 2022-10-12 | Сі-Джей Чеільчетан Корпорейшн | Застосування d-псикози-3-епімерази для продукування d-псикози з d-фруктози |
| CN108239633B (zh) * | 2016-12-26 | 2023-05-05 | 上海立足生物科技有限公司 | 一种催化活性得到提高的d-阿洛酮糖-3-差向异构酶的突变体及其应用 |
| BR112019013853A2 (pt) | 2017-01-06 | 2020-01-28 | Greenlight Biosciences Inc | produção de açúcares isentos de células |
| CN111836889B (zh) * | 2018-01-24 | 2024-02-09 | 松谷化学工业株式会社 | 热稳定性提高的酮糖3-差向异构酶 |
| KR102439295B1 (ko) | 2018-11-30 | 2022-09-02 | 씨제이제일제당 주식회사 | D-사이코스 결정 및 이의 제조 방법 |
| CN110396513B (zh) * | 2019-07-19 | 2022-01-11 | 天津科技大学 | 一种d-阿洛酮糖-3-差向异构酶的突变体及其应用 |
| CN110684762B (zh) * | 2019-11-12 | 2021-03-19 | 南京工业大学 | 一种d-阿洛酮糖-3-差向异构酶突变体及其编码基因、重组载体、重组菌株和应用 |
| CN111019928B (zh) * | 2019-12-11 | 2022-08-16 | 吉林中粮生化有限公司 | D-阿洛酮糖3-差向异构酶的编码基因、载体、重组细胞以及它们的应用 |
| KR102254411B1 (ko) | 2019-12-19 | 2021-05-24 | 대상 주식회사 | 알룰로스 에피머화 효소 변이체, 이의 제조방법 및 이를 이용한 알룰로스의 제조방법 |
| WO2021199445A1 (en) * | 2020-04-03 | 2021-10-07 | Rensselaer Polytechnic Institute | Method for producing sulfated polysaccharide and method for producing paps |
| KR102448351B1 (ko) | 2020-04-27 | 2022-09-28 | 대상 주식회사 | 알룰로스 에피머화 효소 변이체, 이의 제조방법 및 이를 이용한 알룰로스의 제조방법 |
| JP7348457B2 (ja) | 2020-06-03 | 2023-09-21 | ティアンゴン バイオテクノロジー（ティアンジン）カンパニー，リミテッド | プシコース３－エピメラーゼミュータント、それを発現するための遺伝子工学菌、その固定化酵素及び固定化方法 |
| CN111793616B (zh) * | 2020-08-07 | 2022-04-12 | 天津科技大学 | 一种差向异构酶的突变体及其应用 |
| KR20230073739A (ko) | 2021-11-19 | 2023-05-26 | 대상 주식회사 | 열 안정성이 우수한 알룰로스 에피머화 효소 변이체, 이의 제조방법 및 이를 이용한 알룰로스의 제조방법 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100744479B1 (ko) * | 2005-06-01 | 2007-08-01 | 씨제이 주식회사 | 사이코스 에피머화 효소에 의한 사이코스의 생산 방법 |
| GB0512150D0 (en) * | 2005-06-15 | 2005-07-20 | Scottish Crop Res Inst | Production of L-ascorbic acid |
| KR20110035805A (ko) * | 2009-09-30 | 2011-04-06 | 씨제이제일제당 (주) | 사이코스-에피머화 효소의 고정화 및 이를 이용한 사이코스의 제조방법 |
-
2011
- 2011-08-24 KR KR1020110084712A patent/KR101203856B1/ko active IP Right Grant
-
2012
- 2012-08-21 SI SI201230996T patent/SI2749645T1/sl unknown
- 2012-08-21 ES ES12826072.6T patent/ES2626501T3/es active Active
- 2012-08-21 CN CN201280052112.0A patent/CN104160023B/zh active Active
- 2012-08-21 DK DK12826072.6T patent/DK2749645T3/en active
- 2012-08-21 JP JP2014527066A patent/JP6043354B2/ja active Active
- 2012-08-21 US US14/240,647 patent/US9217166B2/en active Active
- 2012-08-21 WO PCT/KR2012/006637 patent/WO2013027999A2/ko active Application Filing
- 2012-08-21 PL PL12826072T patent/PL2749645T3/pl unknown
- 2012-08-21 IN IN1614CHN2014 patent/IN2014CN01614A/en unknown
- 2012-08-21 RS RS20170604A patent/RS56088B1/sr unknown
- 2012-08-21 EP EP17153617.0A patent/EP3192867A1/en not_active Withdrawn
- 2012-08-21 EP EP12826072.6A patent/EP2749645B1/en active Active
- 2012-08-21 PT PT128260726T patent/PT2749645T/pt unknown
- 2012-08-21 HU HUE12826072A patent/HUE035316T2/en unknown
-
2017
- 2017-05-30 HR HRP20170813TT patent/HRP20170813T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CN104160023B (zh) | 2017-03-29 |
| WO2013027999A9 (ko) | 2013-07-11 |
| DK2749645T3 (en) | 2017-08-21 |
| EP2749645A2 (en) | 2014-07-02 |
| WO2013027999A3 (ko) | 2013-05-10 |
| PT2749645T (pt) | 2017-07-28 |
| RS56088B1 (sr) | 2017-10-31 |
| ES2626501T3 (es) | 2017-07-25 |
| US20140199732A1 (en) | 2014-07-17 |
| PL2749645T3 (pl) | 2017-09-29 |
| SI2749645T1 (sl) | 2017-08-31 |
| EP3192867A1 (en) | 2017-07-19 |
| IN2014CN01614A (hr) | 2015-05-08 |
| JP2014525244A (ja) | 2014-09-29 |
| US9217166B2 (en) | 2015-12-22 |
| EP2749645B1 (en) | 2017-04-26 |
| CN104160023A (zh) | 2014-11-19 |
| JP6043354B2 (ja) | 2016-12-14 |
| KR101203856B1 (ko) | 2012-11-21 |
| HUE035316T2 (en) | 2018-05-02 |
| WO2013027999A2 (ko) | 2013-02-28 |
| EP2749645A4 (en) | 2015-06-10 |
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