EA200970212A1 - Варианты пуллуланазы с повышенной продуктивностью - Google Patents

Варианты пуллуланазы с повышенной продуктивностью

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Publication number
EA200970212A1
EA200970212A1 EA200970212A EA200970212A EA200970212A1 EA 200970212 A1 EA200970212 A1 EA 200970212A1 EA 200970212 A EA200970212 A EA 200970212A EA 200970212 A EA200970212 A EA 200970212A EA 200970212 A1 EA200970212 A1 EA 200970212A1
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EA
Eurasian Patent Office
Prior art keywords
pullulanase
new
variants
well
gene sequences
Prior art date
Application number
EA200970212A
Other languages
English (en)
Other versions
EA017712B1 (ru
Inventor
Джордж Инглэнд
Марк КОЛКМАН
Брайан С. Миллер
Каспер Врумен
Original Assignee
ДАНИСКО ЮЭс, ИНК., ДЖЕНЕНКОР ДИВИЖН
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by ДАНИСКО ЮЭс, ИНК., ДЖЕНЕНКОР ДИВИЖН filed Critical ДАНИСКО ЮЭс, ИНК., ДЖЕНЕНКОР ДИВИЖН
Publication of EA200970212A1 publication Critical patent/EA200970212A1/ru
Publication of EA017712B1 publication Critical patent/EA017712B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041—Pullulanase (3.2.1.41)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Изобретение относится к новым вариантам ферментного пептида пуллуланазы, к генным последовательностям, кодирующим указанные новые пептиды, к векторам экспрессии, содержащим эти генные последовательности, а также к организмам, экспрессирующим новые варианты пуллуланазы. Новые варианты пуллуланазы, предлагаемые настоящим изобретением, были изготовлены эмпирически с применением процедур кодонной оптимизации, селективного усечения молекул "дикого типа", а также с замещением избранных аминокислотных остатков. Кроме того, изобретение относится к применению этих новых пептидов пуллуланазы в производстве текстиля, в процессах брожения, в пищевой промышленности и в других отраслях индустрии.
EA200970212A 2006-08-23 2007-08-21 Композиции для разжижения и осахаривания крахмала, основанные на активности пуллуланазы, и способы получения пептидов с активностью пуллуланазы EA017712B1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US83973506P 2006-08-23 2006-08-23
US90324707P 2007-02-23 2007-02-23
PCT/US2007/018523 WO2008024372A2 (en) 2006-08-23 2007-08-21 Pullulanase variants with increased productivity

Publications (2)

Publication Number Publication Date
EA200970212A1 true EA200970212A1 (ru) 2009-08-28
EA017712B1 EA017712B1 (ru) 2013-02-28

Family

ID=38969548

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200970212A EA017712B1 (ru) 2006-08-23 2007-08-21 Композиции для разжижения и осахаривания крахмала, основанные на активности пуллуланазы, и способы получения пептидов с активностью пуллуланазы

Country Status (15)

Country Link
US (2) US7968691B2 (ru)
EP (1) EP2054512B1 (ru)
JP (1) JP2010501181A (ru)
KR (1) KR20090041410A (ru)
CN (2) CN103898080B (ru)
BR (1) BRPI0715619A2 (ru)
CA (2) CA2949536A1 (ru)
CO (1) CO6160247A2 (ru)
EA (1) EA017712B1 (ru)
ES (1) ES2547857T3 (ru)
HK (1) HK1135137A1 (ru)
HU (1) HUE025068T2 (ru)
MX (1) MX2009001717A (ru)
PL (1) PL2054512T3 (ru)
WO (1) WO2008024372A2 (ru)

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US7968691B2 (en) * 2006-08-23 2011-06-28 Danisco Us Inc. Pullulanase variants with increased productivity
WO2011000498A1 (en) 2009-07-01 2011-01-06 Bayer Bioscience N.V. Methods and means for obtaining plants with enhanced glyphosate tolerance
HUE039341T2 (hu) 2013-03-11 2018-12-28 Danisco Us Inc Alfa-amiláz kombinatorikus variációi
WO2015007639A1 (en) 2013-07-17 2015-01-22 Novozymes A/S Pullulanase chimeras and polynucleotides encoding same
EP3063263A1 (en) 2013-10-28 2016-09-07 Danisco US Inc. Large scale genetically engineered active dry yeast
EP3071691B1 (en) 2013-11-20 2019-10-23 Danisco US Inc. Variant alpha-amylases having reduced susceptibility to protease cleavage, and methods of use, thereof
AR102419A1 (es) 2014-10-23 2017-03-01 Novozymes As Variantes de glucoamilasa y polinucleótidos que las codifican
JP6770519B2 (ja) 2014-12-23 2020-10-14 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 酵素により製造されるセルロース
WO2016126294A1 (en) 2015-02-04 2016-08-11 Nanjing Bestzyme Bio-Engineering Co. Ltd. Truncated pullulanases, methods of production, and methods of use thereof
CN107250365A (zh) * 2015-02-19 2017-10-13 丹尼斯科美国公司 增强的蛋白质表达
KR20180117166A (ko) 2016-03-04 2018-10-26 다니스코 유에스 인크. 미생물에서의 단백질 생산을 위한 유전자 조작 리보솜 프로모터
CN105925594A (zh) * 2016-06-13 2016-09-07 广西大学 生淀粉糖化酶及其制备方法与其在生淀粉水解和生料同步糖化发酵产酒精中的应用
DK3571217T3 (da) 2017-01-23 2023-09-18 Danisco Us Inc Fremgangsmåder og sammensætninger til opnåelse af naturlig kompetence i bacillus-værtsceller
FI3585910T3 (fi) 2017-02-24 2024-06-19 Danisco Us Inc Koostumukset ja menetelmät proteiinin tuotannon lisäämiseksi bacillus licheniformiksessa
WO2019089898A1 (en) 2017-11-02 2019-05-09 Danisco Us Inc Freezing point depressed solid matrix compositions for melt granulation of enzymes
WO2019135868A1 (en) 2018-01-03 2019-07-11 Danisco Us Inc Mutant and genetically modified bacillus cells and methods thereof for increased protein production
US20220105204A1 (en) * 2019-02-20 2022-04-07 Duke University Compositions and Methods for the Treatment of Genetic Diseases
CN110184258B (zh) * 2019-06-11 2020-11-06 南京林业大学 一种普鲁兰酶突变体
CN110791513B (zh) * 2019-11-15 2021-05-25 中国科学院南海海洋研究所 一种密码子优化的玉足海参α-淀粉酶基因及其重组表达载体与应用
CN111662933B (zh) * 2020-06-24 2023-03-24 江南大学 一种利用重组酵母进行高支链淀粉酒精发酵的方法
EP4387982A2 (en) * 2021-08-20 2024-06-26 Danisco US Inc. Methods and compositions for enhanced protein production in bacillus cells
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

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Also Published As

Publication number Publication date
CN101506360B (zh) 2014-06-11
EP2054512A2 (en) 2009-05-06
US8354101B2 (en) 2013-01-15
US20110281326A1 (en) 2011-11-17
EP2054512B1 (en) 2015-04-22
WO2008024372A3 (en) 2008-04-24
CN103898080A (zh) 2014-07-02
CA2663948C (en) 2017-01-03
HUE025068T2 (en) 2016-01-28
HK1135137A1 (en) 2010-05-28
JP2010501181A (ja) 2010-01-21
ES2547857T3 (es) 2015-10-09
BRPI0715619A2 (pt) 2013-06-25
EA017712B1 (ru) 2013-02-28
KR20090041410A (ko) 2009-04-28
CA2663948A1 (en) 2008-02-28
CN101506360A (zh) 2009-08-12
US7968691B2 (en) 2011-06-28
CN103898080B (zh) 2017-04-12
CO6160247A2 (es) 2010-05-20
MX2009001717A (es) 2009-02-25
PL2054512T3 (pl) 2015-09-30
US20080108126A1 (en) 2008-05-08
WO2008024372A2 (en) 2008-02-28
CA2949536A1 (en) 2008-02-28

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MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

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