The seedling-cultivating method of a kind of Damask Rose
Technical field
The present invention relates to the seedling-cultivating method of a kind of Damask Rose, belong to seedling propagation technical field.
Background technology
Damask Rose (Rosadamascena) is shrub, originates in Asia Minor, and in southern Europe, cultivation is long.Damask Rose pattern is gorgeous, fragrance overflows people, not only can make tracery wall, Lv Qiang, flower bed pad and garden and view and admire edible flowers and trees, and has promoting blood circulation and removing blood stasis, swelling and pain relieving, beautifying face and moistering lotion effect; The spices that simultaneously the Ye Shi world is famous, is again the batching of smoked tea, wine brewing, diet and medicine, has very high economic worth.The Rosa Damascana extracted from Damask Rose fresh flower, is rich in high unit vitamin C, has quality gentleness, pleasant aroma, strong and lasting characteristic; Also there is pressure of releiving, desalination scar, anti-quick, Bearberry Extract simultaneously.Except above-mentioned advantage, function, Damask Rose essential oil is nursed one's health premenstrual syndrome, physiological period, heart, stomach, liver, uterine function, treatment eczema, conditioning in addition and is shunk the functions such as capilary, is the superfine product in Rosa Damascana.
The seedling-cultivating method of China Damask Rose is mainly cuttage and seedling culture.Publication number is that the Chinese invention patent of CN102612971A discloses a kind of rose cuttage breeding method, comprises the following steps: the process in (1) seedbed: dig the cuttage cave that 16 ~ 27cm is dark, the soil containing mixed-matrix is used in bottom, and soil 4 ~ 6cm is thick; Described cuttage acupuncture point is on the ridge of 40 ~ 60cm, wide 80 ~ 120cm in spacing, and the spacing in cuttage cave is 40 ~ 60cm; (2) process of cuttage bar; (3) cuttage: inserted in cuttage cave by the branch handled well, reclaimed soil also waters permeable; (4) manage after cuttage: temperature remains on 18 ~ 28 DEG C, the water content in seedbed remains on 60 ~ 80%, and relative air humidity is 65 ~ 85%, after cuttage, every day waters with taking root liquid, after 10 ~ 15 days, stop pouring taking root liquid, allow cuttage seeding accept illumination gradually.
Above-mentioned rose cuttage breeding method, it is incomplete that cuttage branch lacks root system, nutrition organs, and all more weak to the absorbing capacity of the nutritive element in matrix and moisture, self healing ability of cuttage branch is weak, and survival ability is weak.Be inserted in by cuttage branch on railway carriage or compartment, ridge, the water-holding capacity in railway carriage or compartment, ridge is weak, needs regularly to keep the skin wet in soil the moisture that could keep in soil.But, regularly water and keep the method for the moisture in soil that the soil moisture fluctuation around cuttage branch can be caused large, the moisture that cuttage branch absorbs in soil is unbalanced, affects the vital movement of cuttage branch, and then causes cuttage branch rooting rate survival rate that is low, cuttage seeding low.
Further, in branch cutting rooting process, different growing stage, cuttage branch has different specific requirements to the air humidity in soil humidity and seedling growth greenhouse.And above-mentioned rose cuttage breeding method, the water content controlling seedbed after only giving cuttage is 60 ~ 80%, relative air humidity is 65 ~ 85%, the water content in this seedbed and relative air humidity do not design according to the growth characteristics of cuttage seeding, finally cause the rooting rate of cuttage seeding low, the survival rate of cuttage seeding is low.
Summary of the invention
The object of the invention is to overcome the above-mentioned deficiency that cuttage seeding surrounding soil existing in prior art, large, the slotting rear soil humidity of soil humidity fluctuation range and the management of cuttings surrounding air relative moisture can not adapt to the growth characteristics of cuttage seeding, there is provided the seedling-cultivating method of matrix after a kind of cuttage, soil moisture constant, this method significantly can promote rooting rate, the survival rate of cuttings;
The present invention also aims to insert rear soil humidity, cuttings ambient air humidity by regulating, improving the rooting rate of cuttings, survival rate further.
In order to realize foregoing invention object, the invention provides following technical scheme:
A seedling-cultivating method for Damask Rose, comprises the maintenance management after (1) seedling bed process, (2) cuttings process, (3) cuttage (4) cuttage, wherein,
The seedling bed of described step (1) is treated to: matrix is loaded floating seedling tray, is then placed on nursery water bed by floating seedling tray, is sprayed to matrix and soaks completely;
The cuttage of described step (1) for: in the matrix of floating seedling tray described in the cuttings inserting step (1) after step (2) is processed, after whole cuttage, with disinfectant to cuttings, matrix and floating seedling tray sterilization, the temperature regulating nursery pond water bed is 20 ~ 30 DEG C, regulates cuttings ambient air humidity to be greater than 90%.
The seedling-cultivating method of above-mentioned Damask Rose, described floating seedling tray is a kind ofly may float on seedling culture hole plate waterborne, and seedling culture hole plate comprises several cave grooves (determining according to seedling-cultivating tray size), each cave trench bottom is provided with through hole.By in the cave groove of the matrix packing that configures and floating seedling tray, then floating seedling tray is placed on water bed floating, matrix absorbs the water in nursery pond water bed by described through hole, by arranging the degree of depth that floating seedling tray submerges in water, can regulate the humidity of floating seedling tray mesostroma.By cuttings cuttage in floating seedling tray, the degree of depth that floating seedling tray floats the water bed that submerges is constant, and the humidity of cuttings cuttage surrounding substrate can keep constant, the moisture stable of the growing environment of such cuttings, and the rooting rate of cuttage seeding is high, survival rate is high.
As preferred version of the present invention, the maintenance management after described step (4) cuttage is, regulates described matrix and cuttings ambient air temperature to be 20 ~ 30 DEG C; The intensity of illumination that cuttings accepts is shine the illumination that light transmittance is the sunshade net of 90% consecutive days;
By following four-stage, regulate soil humidity and cuttings ambient air humidity:
A () inserts latter 1st ~ 7 days:
Humidity: air humidity > 90%, soil humidity is 70 ~ 80%;
B () inserts latter 8th ~ 15 days:
Humidity: air humidity > 90%, soil humidity is 60 ~ 70%;
C () inserts latter 16th ~ 20 days:
Humidity: air humidity 70 ~ 90%, soil humidity is 50 ~ 60%;
D () inserts rear after 20th day: air humidity is identical with natural air humidity, and soil humidity is 50 ~ 60%.
Above-mentioned air humidity is the air humidity contacted with cuttings.
In cuttage and seedling culture process, cuttings is grown to cuttage seeding generally can be divided into four-stage, this four-stage, and cuttings has strict requirement to soil humidity and cuttings ambient air humidity.For this growth characteristics, inventor has devised slotting rear maintenance management as above.First stage: after cuttings cuttage 1st ~ 7 days, this stage is the cuttings Callus formation phase.This, cuttings did not have root system in period, and the water absorbing capacity of cuttings to surrounding is weak, and in order to ensure moisture in cuttings, inventor have employed the method for increasing income and decreasing expenditure and ensure that water balance in cuttings.Described method of increasing income and decreasing expenditure refers to, makes the value that soil humidity keeps higher, increases cuttings to the absorption of moisture in matrix, increases the transpiration that air humidity reduces cuttings blade simultaneously.Research through inventor finds, this stage need keep air humidity higher than 90%, and soil humidity is that 70 ~ 80% cuttings absorb moisture and export water tariff collection dynamic equilibrium, and the callus healing of cuttings is fast.Air humidity lower than 90% time, the moisture evaporation of cuttings blade is fast, and can destroy the moisture dynamic equilibrium in cuttings, cause cuttings dehydration, and then cause rooting percent low, cuttage seeding survival rate is low; Simultaneously soil humidity be lower than 70% time, being short of power of cuttings absorption moisture, also can destroy the moisture dynamic equilibrium in cuttings, cause cuttings dehydration, and then cause rooting percent low, cuttage seeding survival rate is low; And soil humidity can cause matrix permeability to weaken higher than 80%, the part anuerobic respiration that cuttings is arranged in matrix is strengthened, and anaerobic respiration product alcohol can cause cuttings to be rotted.Second stage: after cuttings cuttage 8th ~ 15 days, this stage is young root vegetative period of cuttings, and this, cuttings started to take root in period, and when taking root, cell proliferation needs comparatively multi-energy, but because cuttings nutrition organs is not also grown completely, every vital movement of cuttage seeding is still more weak.This stage needs the humidity suitably reducing matrix, improves the permeability of matrix.So both be conducive to the aerobic respiration increasing cuttage rooting position, and for cell proliferation provides energy to ensure, suitably can have weakened again the vital movement of cuttings, reduced the energy ezpenditure of cuttings, ensure the nutrition supply of cuttings to this process of having taken root; Meanwhile, although this in period cuttings water absorbing capacity strengthen, the humidity of matrix can corresponding reduction, still needs maintenance cuttage seeding surrounding air to have higher humidity.Inventor is through to study for a long period of time discoverys, and in the young root vegetative period of cuttings, maintenance soil humidity is 60 ~ 70%, air humidity > 90%, and the rooting rate of cuttings is high, described cuttage seeding namely take root after cuttings.Phase III: after inserting 16th ~ 20 days, this stage is cuttage rooting animated period, and this cuttage seeding continues propagation young root in stage, and young root root system is bred in a large number simultaneously, root system grows up long thick.The water absorbing capacity of cuttage seeding significantly promotes, root respiration significantly strengthens, humidity in further reduction matrix, improve the permeability of matrix, not only can improve the oxygen content in matrix, strengthen the respiration of root system, the heat-sinking capability of matrix can also be accelerated, reduce the temperature around root system, avoid root system to be damaged, significantly can promote the survival rate of cuttage seeding.Further, in this stage, the water absorbing capacity of cuttage seeding strengthens, cuttage seeding root also significantly promotes the absorption of nutrient ingredients ability in matrix, reduce the humidity of air, the transpiration of cuttage seeding blade can be strengthened, strengthen the absorbing capacity of cuttage seeding root system to matrix Middle nutrition material and water.Inventor finds, in this period, soil humidity is 50 ~ 60%, and the humidity of cuttage seeding surrounding air is that the rooting rate of 70 ~ 90% cuttage seeding is high, and the well developed root system of cuttage seeding, the survival rate of cuttage seeding is the highest.Fourth stage, after inserting, after the 20th day, the seedling phase of taking root completes, and cuttage seeding nutrition organs is grown completely, hardening can be carried out according to normal bad border naturally, namely this, air humidity was identical with natural air humidity in period, and soil humidity still keeps 50 ~ 60%, can transplant or sell after hardening.
As preferred version of the present invention, described cuttings is semi-lignified spray.The spray tissue children of semi-lignified is tender and containing abundant somatotropin and lyoenzyme, the activation of enzyme is vigorous, is conducive to cuttings and forms callus and take root, significantly can promote the rooting rate of cuttings and the survival rate of cuttage seeding.
As preferred version of the present invention, the long 10 ~ 15cm of described cuttings.Preferred, the length of described cuttings is 10cm.In the period that cuttings root system development is incomplete, namely the above-mentioned first stage is to the phase III, the absorption of cuttings to moisture and nutritive element is all very weak, the photosynthesis of cuttings blade is weak, every vital movement of cuttings mainly maintains by the nutriment in cuttings, when the length of cuttings is less than 10cm, the nutriment stored in cuttings is few, can because the not enough youngster of nutrient supply is dead in cuttings cuttage process; When the length of cuttings is greater than 15cm, the young shoot in cuttings is many, and after the long young leaves of sprouting, the transpiration of cuttings is strengthened, and cuttings root system development is complete, and the absorbing capacity of cuttings to moisture is weak, and cuttings can be caused to lack moisture, dryly to wither, even dead.
Preferred further, described cuttings upper end otch is flat mouth, and there are 43 ~ 47 degree of angles cuttings inferior end notch tangent plane and cuttings sagittal plane.Further preferred, the angle of cuttings inferior end notch tangent plane and cuttings sagittal plane is 45 °.Cuttings upper end otch is flat mouth, and otch area is little, can reduce the transpiration of cuttings stem moisture, reduces the generation of damage by disease and insect simultaneously; There are 43 ~ 47 degree of angles cuttings inferior end notch tangent plane and cuttings sagittal plane, so both increase the contact area of cuttings and matrix, promote that cuttings is to the absorption of moisture in matrix, can reduce again the damage by disease and insect incidence probability of cuttings.
As preferred version of the present invention, described cuttings retains 2 ~ 4 blades.Cuttings retains the photosynthesis that 2 ~ 4 blades can increase cuttings, extra-nutrition material, can reduce again the transpiration on blade face, keeps moisture needed for life in cuttings.
As preferred version of the present invention, described matrix is the peat composed of rotten mosses and perlitic mixture, and the described peat composed of rotten mosses and described perlite are that the ratio of 1 ~ 2:1 mixes by volume.The peat composed of rotten mosses and perlite are that after the ratio of 1 ~ 2:1 mixes, matrix permeability is good by volume, can improve the oxygen content of matrix energy, be conducive to the growth of cuttings.
Preferably, described disinfectant is 800 times of carbendazim, and namely carbendazim and water are the solution of the proportional arrangement of 1:800 by volume.Damask Rose seedling growing process of the present invention, carries out disinfection to cuttings with 800 times of carbendazim, and the Disinfection Effect of cuttings well not easily disease occurs, and disinfectant can not damage the nutrition organs of cuttings, and the rooting rate of cuttings is high, and root system quality is good.
Preferably, described root-growing agent is the IBA of 50 ~ 200ppm.By the IBA process cuttings of 50 ~ 200ppm, cuttings rooting rate high, root system quality is good.When the concentration of IBA is less than 50ppm, the concentration of root-growing agent is low, and the amount of taking root of cuttings is few and root system is short and thin; When the concentration of IBA is greater than 200ppm, the concentration of root-growing agent is high, can suppress the growth of cuttings root system, and the amount of taking root of cuttings is few and root system is short and thin.
As preferred version of the present invention, step (4) is described inserts in rear maintenance management process, every spray in 7 days 800 times of carbendazim sterilization.In hot and humid environment, cuttings is after 800 times of carbendazim sterilizations, and in general 7 days, cuttings disease can not occur; From after sterilization the 8th day, easily there is disease infection in cuttings otch, damage location or newborn position.Cuttings generation disease infection can be prevented every spray in 7 days 800 times of carbendazim sterilization, cuttings histoorgan will be caused impaired because of sterilization and too much simultaneously.
Compared with prior art, beneficial effect of the present invention:
1, the seedling-cultivating method of Damask Rose of the present invention, by cuttings cuttage in floating seedling tray, floating the degree of depth of the water bed that submerges, can regulate the humidity of floating seedling tray mesostroma by arranging floating seedling tray.Regulated the humidity of matrix by floating seedling tray, the humidity of matrix can be made to remain in a stability range, the moisture stable of the growing environment of such cuttings, there will not be humidity to change fluctuation phenomenon greatly, the rooting rate of cuttings is high, and cuttage seeding survival rate is high.
2, maintenance management process after inserting, according to the growth characteristics of the four-stage that cuttings is grown, design adapts to soil humidity and the air humidity of cuttings growth, make in cuttings, to keep sufficient moisture, ensure every vital movement of cuttings, rooting percent is high, and cuttage seeding survival rate is high.
Embodiment
Below in conjunction with test example and embodiment, the present invention is described in further detail.But this should be interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to following embodiment, all technology realized based on content of the present invention all belong to scope of the present invention.
In various embodiments of the present invention and comparative example, the measurement of described temperature and humidity, all adopts " agate Hygrothermograph AR827 is wished in Hong Kong " to measure.
Embodiment 1
The seedling-cultivating method of the Damask Rose of the present embodiment, comprises the following steps:
(1) seedling bed process:
The ratio being 1:1 by volume by the peat composed of rotten mosses and perlite mixes, and is configured to matrix;
The matrix configured loaded in floating seedling tray, described floating seedling tray is the seedling culture hole plate that foamed plastics is made, and seedling culture hole plate is divided into 60 ~ 100 cave grooves, and each cave trench bottom is provided with through hole, and matrix is loaded in the groove of cave, and stromal thickness is 6 ~ 7cm;
Then be placed on the water bed of nursery pond by floating seedling tray, be sprayed to matrix and soak completely, matrix can from the water the through hole intussusception water bed of each cave trench bottom; Floating seedling tray submerges the humidity of the depth adjustment floating seedling tray mesostroma in water bed, simultaneously by regulating the temperature of the lower water temperature matrix of water bed;
(2) cuttings process:
Before morning 9, clip semi-lignified spray is also transported to operating space in time, and operating space is positioned at shade, after semi-lignified spray is transported to operating space, to the spraying of semi-lignified spray, makes semi-lignified spray surface keep moistening;
Above-mentioned semi-lignified spray is cut into the cuttings that 10 ~ 15cm is long, cuttings upper end otch is flat mouth, and there are 43 ~ 47 ° of angles cuttings inferior end notch tangent plane and cuttings sagittal plane;
Cuttings is stayed 2 ~ 4 blades;
Cuttings 50 strain sheared is one group, and soak 3min take out putting into 800 times of carbendazim after often organizing binding, the IBA then cuttings after sterilization being put into 50ppm soaks 30min, takes out;
(3) cuttage:
With the matrix jack of waddy in step (1) in the groove of cave of 0.5 ~ 2mm thicker than cuttings, the degree of depth of jack is the half of matrix, about 3.5cm; By the cuttings handled well in step (2) insert matrix jack in and with hand by matrix compacting, open spraying apparatus immediately, be sprayed to cuttings blade moistening;
After whole cuttage, with the carbendazim of 800 times, cuttings, seedling-cultivating tray, matrix are carried out disinfection sterilizing; And regulate that the temperature of nursery pond water bed is 24 ~ 26 DEG C, air humidity is 90% ~ 100% in seedling growth greenhouse;
(4) maintenance management after cuttage:
Matrix and nursery pond air temperature is regulated to be 20 ~ 30 DEG C; The intensity of illumination regulating cuttings to accept is shine the illumination that light transmittance is the sunshade net of 90% consecutive days;
In maintenance management process, regulate soil humidity and cuttings ambient air humidity by following four-stage:
A () inserts latter 1st ~ 7 days, the Callus formation phase: daytime, every 10min sprayed once, a 15s; Once, a 20s, makes to remain water membrane above blade in 15 ~ 20min spraying at night; Floating seedling tray mesostroma submerges 2.8 ~ 3.2cm in water;
Detect: air humidity > 90%, soil humidity is 70 ~ 80%;
(b) insert after 8th ~ 15 days, young root vegetative period: 15 ~ 20min spraying once, a 15s; Moisture film on blade is kept to be greater than 2/3 of blade area; Floating seedling tray mesostroma submerges 2.4 ~ 2.8cm in water;
Detect: air humidity > 90%, soil humidity is 60 ~ 70%;
C () inserts latter 16th ~ 20 days, cuttage rooting animated period: on blade, moisture evaporates and starts spraying, a 15s; Floating seedling tray mesostroma submerges 2.0 ~ 2.4cm in water;
Detect: air humidity 70 ~ 90%, soil humidity is 50 ~ 60%;
D () inserts latter 20th ~ 30 days, the seedling phase of taking root completes:
Ventilate in seedling growth greenhouse, simultaneously at 10 in the morning to afternoon 3 point, every spray half an hour once, a 15s, other times stop spraying; Floating seedling tray mesostroma submerges 2.0 ~ 2.4cm in water;
Detect: in seedling growth greenhouse, air humidity is identical with extraneous natural air humidity, soil humidity 50 ~ 60%.
Illustrate: in above-mentioned maintenance management process, spray timings and the time interval be sunshine strong, temperature higher than the condition design of 25 DEG C, when intensity of sunshine and temperature are lower than 25 DEG C, suitably can reduce spray timings, to ensure the air humidity in each stage.
In the present embodiment, cuttings totally 70000 strains of cuttage, nursery stage terminates (after namely inserting the 30th day), and cuttings of taking root is 56000 strains, and the rooting rate of cuttings is 80%; Cuttage seeding 54000 strain survived, the survival rate of cuttage seeding is 77%.
Comparative example
The seedling-cultivating method of the Damask Rose of this comparative example, comprises the following steps:
(1) seedling bed process:
The ratio being 1:1 by volume by the peat composed of rotten mosses and perlite mixes, and is configured to matrix;
The matrix configured loaded in floating seedling tray, described floating seedling tray is the seedling culture hole plate that foamed plastics is made, and seedling culture hole plate is divided into 60 ~ 100 cave grooves, and each cave trench bottom is provided with through hole, and matrix is loaded in the groove of cave, and stromal thickness is 6 ~ 7cm;
Then be placed on the water bed of nursery pond by floating seedling tray, be sprayed to matrix and soak completely, matrix can from the water the through hole intussusception water bed of each cave trench bottom; Floating seedling tray submerges the humidity of the depth adjustment floating seedling tray mesostroma in water bed, simultaneously by regulating the temperature of the lower water temperature matrix of water bed;
(2) cuttings process:
Before morning 9, clip semi-lignified spray is also transported to operating space in time, and operating space is positioned at shade, after semi-lignified spray is transported to operating space, to the spraying of semi-lignified spray, makes semi-lignified spray surface keep moistening;
Above-mentioned semi-lignified spray is cut into the cuttings that 10 ~ 15cm is long, cuttings upper end otch is flat mouth, and there are 43 ° ~ 47 ° angles cuttings inferior end notch tangent plane and cuttings sagittal plane;
Cuttings retains 2 ~ 4 blades;
Cuttings 50 strain sheared is one group, and soak 3min take out putting into 800 times of carbendazim after often organizing binding, the IBA then cuttings after sterilization being put into 50ppm soaks 30min, takes out;
(3) cuttage:
With the matrix jack of waddy in step (1) in the groove of cave of 0.5 ~ 2mm thicker than cuttings, the degree of depth of jack is the half of matrix, about 3.5cm; By the cuttings handled well in step (2) insert matrix jack in and with hand by matrix compacting, open spraying apparatus immediately, be sprayed to cuttings blade moistening;
After whole cuttage, with the carbendazim of 800 times, cuttings, seedling-cultivating tray, matrix are carried out disinfection sterilizing; And regulate that the temperature of nursery pond water bed is 24 ~ 26 DEG C, air humidity is 90% ~ 100% in seedling growth greenhouse;
(4) maintenance management after cuttage:
Regulate the temperature of matrix and air between 20 ~ 30 DEG C, the illumination that cuttings accepts is shine the illumination that light transmittance is the sunshade net of 90% consecutive days;
Thermometer in seedling growth greenhouse and moisture meter are regularly checked to humidity in soil humidity and seedling growth greenhouse, when air humidity is less than 70%, cuttings is sprayed, a 20s; In seedling growth greenhouse air humidity higher than 85% time, seedling growth greenhouse ventilates and reduces air humidity in seedling growth greenhouse, makes air humidity in seedling growth greenhouse between 65 ~ 85%;
Floating seedling tray mesostroma is regulated to submerge 2.4 ~ 3.2cm in water; Make the humidity of floating seedling tray mesostroma between 60 ~ 80%.
In this comparative example, cuttings totally 10000 strains of cuttage, nursery stage terminates (after namely inserting the 30th day), and cuttings of taking root is 5000 strains, and the rooting rate of cuttings is 50%; Cuttage seeding 4000 strain survived, the survival rate of cuttage seeding is 40%.
Learnt by the experimental data of embodiment 1 and comparative example, in embodiment 1, the rooting rate of cuttings is 80%, and cuttage seeding survival rate is 77%; And in comparative example, the rooting rate of cuttings is 50%, cuttage seeding survival rate is 40%, and in embodiment 1, the rooting rate of cuttings and the survival rate of cuttage seeding distinguish high 60% and 92.5% than the rooting rate of the cuttings of comparative example and the survival rate of cuttage seeding.Damask Rose seedling-cultivating method of the present invention, maintenance management process after inserting, according to the growth characteristics of the four-stage that cuttings is grown, design adapts to soil humidity and the air humidity of cuttings growth, and the rooting rate of cuttings and cuttage seeding survival rate are significantly improved.
Further, embodiment 1 adopts floating cuttage and seedling culture method maintenance cuttings, and in maintenance management process, the soil humidity of detection is constant.Avoiding cuttings because of ambient water divides fluctuation large, and causes the phenomenon that the dead or cuttage rooting of cuttings dehydration rots.Compared in prior art, on railway carriage or compartment, ridge, the survival rate of the cuttage seeding of cuttage and seedling culture is 20%, and the cuttage seeding survival rate of the embodiment of the present invention 1 can be increased to 77%.Floating cuttage and seedling culture method of the present invention, by filling matrix in floating seedling tray, regulating soil humidity constant, making the rooting rate of cuttings and the seedling percent of cuttage seeding improve nearly 3 times.