CN103571962B - 一种多切刻酶位点介导的核酸恒温扩增检测方法 - Google Patents
一种多切刻酶位点介导的核酸恒温扩增检测方法 Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/119—Strand displacement amplification [SDA]
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US10604790B2 (en) * | 2014-12-24 | 2020-03-31 | Abbott Laboratories | Sequence conversion and signal amplifier DNA cascade reactions and detection methods using same |
CN105176971A (zh) * | 2015-08-14 | 2015-12-23 | 青岛科技大学 | Bst DNA聚合酶在RNA扩增中的应用 |
CN108913736B (zh) * | 2018-07-10 | 2021-08-24 | 中国海洋大学 | 单链寡核苷酸的制备方法 |
CN110607355A (zh) * | 2019-02-18 | 2019-12-24 | 华东理工大学 | 一种基于Cas9切口酶偶联DNA聚合酶的恒温核酸检测分析方法及试剂盒 |
CN110157777B (zh) * | 2019-05-27 | 2020-08-11 | 福州大学 | 一种基于发卡结构变换的放大型荧光生物传感器及制备方法 |
CN110408678A (zh) * | 2019-07-26 | 2019-11-05 | 宁儿医院股份有限公司 | 单管型多重微生物检测体系及其即时检测方法 |
CN113736858B (zh) * | 2020-05-28 | 2024-05-10 | 香港科技大学 | 一种环状寡核苷酸探针介导的核酸扩增子的实时监测方法 |
CN112695076A (zh) * | 2021-02-05 | 2021-04-23 | 青岛农业大学 | 一种核酸恒温扩增定量检测方法 |
CN112941154B (zh) * | 2021-02-21 | 2022-09-09 | 中南大学 | 一种分子信标探针及其在制备检测circBART2.2制剂中的应用和试剂盒 |
CN113624980B (zh) * | 2021-08-09 | 2023-06-13 | 四川大学华西医院 | 基于识别诱导的恒温扩增技术对蛋白质进行检测的方法及试剂盒 |
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CN101368209A (zh) * | 2008-09-12 | 2009-02-18 | 青岛科技大学 | 一种基于核酸适体和pcr扩增来检测目标分子的方法 |
US8017328B2 (en) * | 2008-11-21 | 2011-09-13 | Agilent Technologies, Inc. | Genome partitioning using a nicking endonuclease |
CN103088128B (zh) * | 2013-01-09 | 2015-11-18 | 华中科技大学 | 一种microRNA的二次循环扩增检测方法及试剂盒 |
CN103215366A (zh) * | 2013-04-25 | 2013-07-24 | 中国科学院广州生物医药与健康研究院 | 基于核酸酶和发夹dna探针的等温信号扩增的多重基因分型的检测方法 |
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