CN102282995A - Cold-resistant excellent lance-leaved red anise strain cutting propagation technology - Google Patents
Cold-resistant excellent lance-leaved red anise strain cutting propagation technology Download PDFInfo
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- CN102282995A CN102282995A CN201110174469A CN201110174469A CN102282995A CN 102282995 A CN102282995 A CN 102282995A CN 201110174469 A CN201110174469 A CN 201110174469A CN 201110174469 A CN201110174469 A CN 201110174469A CN 102282995 A CN102282995 A CN 102282995A
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Abstract
The invention relates to a cold-resistant excellent lance-leaved red anise strain cutting propagation technology for evergreen broad-leave ornamental tree species illicium lanceolatum, which comprises the steps of sterilizing a cutting pond; mixing mediums which consist of peat soil, pearlite, sand and vermiculite according to volume ratio of 1:1:1:1, sterilizing a seedling bed by using potassium permanganate solution with concentration being 0.5 percent, sterilizing the mediums by using 50% Mildothane 800-time solution and keeping the thickness of cutting mediums to be 30cm; selecting annual shoots at the middle part of the sunny side of a crown, keeping the length of the shoots to be 10cm to 12cm and cutting off one half of leaves at the top end of the shoots for cutting; using IAA50ppm+IBA50ppm to treat scions and soaking 3cm-long to 4cm-long lower part of the shoots for cutting for 24 hours; keeping cutting depth to be 4cm to 5cm, keeping cutting density with row spacing being 6cm * 8cm and using a sprinkling can to spray water to enable the shoots for cutting to be in full contact with the mediums; and arranging a sunshade net and an automatic spray device above the cutting pond to reduce illumination intensity and increase air humidity, and in the first month after cutting, keeping daytime relative humidity above 65 percent to 80 percent. The survival rate of cut shoots can reach 80 percent. By using the annual shoots and after hormone treatment, the propagation of cold-resistant excellent lance-leaved red anise strains can be conducted rapidly, the method is simple, the operation is simple, the survival rate is high and the like. The technology not only can provide technical supports and excellent strain seedlings for the popularization and the application of lance-leaved red anises which are evergreen broadleaved ornamental trees, but also can provide references for the cutting propagation of the cold-resistant excellent strains of other trees.
Description
Technical field
The invention belongs to the plant asexual multiplication technology field, be specifically related to the cold-resistant excellent strain cuttage breeding method of the red fennel of evergreen broad-leaved shrub lanceolata.
Background technology
The red fennel of lanceolata is evergreen dungarunga of Winteraceae or shrub, and blade is emerald green all the year round, the long and narrow bar shaped of blade, Chang Jisheng branch top forms dense tree crown, the common yellow green of sprout; yellowish-brown or bronzing when Follicle radish fruit is ripe, and endocarp often takes on a red color, and strong anistree fragrance is arranged; Bright red or the shiny red of pattern, the tepal meat, glittering and translucent, have aesthetic feeling, under the setting off of emerald green blade, very dazzlingly beautiful.This kind of disease resistance is strong, during introducing and planting, does not find that the disease worm causes harm.Under the low temperature in Nanjing-9 ℃, blade is not seen obvious freeze injury; The red anisyl of lanceolata originally can adapt to weather, soil and the biotic factor in area, Nanjing, can normally nourish and grow normally, and show higher ornamental value.
Nanjing under the northern fringe weather conditions of subtropics, winter cold, broad-leaved evergreen kind kind is less, and cold-resistant evergreen broad-leaved Landscape Tree Species is had extremely strong demand; The red fennel of lanceolata is introduced a fine variety in Zhong Shan botanical garden, Nanjing in early days since the eighties in last century, by nearly 30 years introducing and planting, the different individual plant growth of the red fennel of the lanceolata of field planting performance difference in the botanical garden, high growth and cold resistance all have bigger difference, be grown in the individual plant in the arboretum, growth rate is very fast, is thick with leaves the tree crown rounding, blade is not seen the freeze injury symptom in the winter time, cold resistance is stronger, and exhibits excellent is a good cold-resistant individual plant that development potentiality is relatively arranged.Owing to plugged ear is to be come by the direct differentiation and development of somatic cell, the merit that can keep parent, utilize this characteristics, the preferably cold-resistant individual plant of the red fennel of evergreen broad-leaved seeds lanceolata is carried out cottage propagation, not only help this and keep a good cold-resistant individual plant cold resistance, can also carry out fast breeding, to enlarging the Landscape Application zone of the red fennel of lanceolata it, important and practical meanings is arranged, and have wide market application prospect.How this paper explanation utilizes artificial cottage propagation technology to enlarge the breeding quantity of cold-resistant excellent strain, satisfies the Landscape Application demand.
At present, yet there are no report,, also do not appear in the newspapers its patent application of carrying out cottage propagation to the red fennel of lanceolata cold-resistant excellent strain carrying out cottage propagation.
Summary of the invention
Problem to be solved by this invention provides the cold-resistant excellent strain cuttage breeding method of the red fennel of a kind of lanceolata, this method can not only keep the cold-resistant proterties of the excellent strain of the red fennel of lanceolata, can also the resist cold quick breeding of excellent strain provides cold resistance strong, the high quality seedling that ornamental value is high; Have that method is simple, workable, the characteristics that cuttage survival rate is high.
The red fennel of the lanceolata of the present invention excellent strain cuttage breeding method that resists cold may further comprise the steps:
(1) preparation of matrix: in the slotting machine of nursery, Zhong Shan botanical garden, Nanjing, the cutting medium that tiling configures, the main component peat soil of matrix: perlite: sand: vermiculite=1: 1: 1: the mixed-matrix of 1 (volume ratio), thickness is 30cm, earlier with concentration be 0.5% potassium permanganate to nursery bed disinfection, carry out the sterilization of matrix with 800 times of liquid of thiophanate methyl of 50% again.Matrix in the slotting machine will want water fully to soak into before cuttage, and cuttage will be at 2～3 days, just can carry out after the property of medicine volatilization.
(2) gather plugged ear: the present invention mainly adopts the early summer plum rain season to carry out the cuttage of spray, and the time is in late June.Gather the current-year branch at tree crown sunny side middle part from cold-resistant individual plant, base portion band heel (the biennial branch of a very short part), length is 10～12cm.The spray of adopting back of giving birth to is then pruned, because the blade of the red fennel of lanceolata is longer, upper blade all cuts off half, helps alleviating transpiration, favourable surviving.
(3) HORMONE TREATMENT plugged ear: in order to compare the cuttage survival rate under the different disposal condition, we handle plugged ear with different hormones or hormone combinations, we have used IBA50ppm, IAA50ppm, IAA50ppm+IBA50ppm and clear water contrast totally 4 processing respectively, and the processing time is 24h.
(4) cuttage: the plugged ear that immersion treatment is crossed, carry out cuttage on slotting machine, the cuttage degree of depth is 4-5cm, and cutting density is seeding row spacing 6cm * 8cm, will spray water with watering can after inserting, and makes between plugged ear and the matrix fully to contact; 1000 times of liquid of spray carbendazim carry out disinfection after the cuttage; Sunshade net and automatic sprayer are installed above the cuttage pond, are reduced intensity of illumination, increase air humidity, avoid the plugged ear dehydration.
(5) management after the cuttage: previous month after cuttage, carry out comparatively strict moisture management, and the humidity of looking weather condition and cutting bed increases and decreases the number of times of spraying, the relative air humidity on daytime remains on more than 65～80%.After one month and the plant of other cuttage carry out same management, regularly spray.The regular check situation of taking root, in the ordinary course of things, 7 days begin to occur callus after the cuttage, have new root to send after 15 days, after 24 days, have 60% plugged ear to send new root.
Characteristics such as the present invention adopts and gives birth to spray then, through after the HORMONE TREATMENT, can carry out the breeding of the cold-resistant excellent strain of the red fennel of lanceolata fast, and method is simple, and processing ease has less investment, and output is big are the breeding fine individual plants, keep the better approach of merit,
Embodiment
With the spray of hormone IBA50ppm and the red fennel of IAA50ppm+IBA50ppm processing lanceolata, cuttage survival rate reaches 70% and 80%, can satisfy the demand in the production; IAA50ppm and clear water control treatment, survival rate only are 47.5% and 30%, and back two kinds of hormone combinations and clear water Processing Example are further specifying optimization process.
Embodiment 1
Late June, in the cold-resistant excellent strain that chooses, gather the current-year branch at tree crown sunny side middle part the morning of selection cloudy day or light rain, base portion band heel (the biennial branch of a very short part), length is 10～12cm.The spray of adopting back of giving birth to is then pruned, because the blade of the red fennel of lanceolata is longer, upper blade all cuts off half.Cuttage is on the slotting machine of sterilizing, and cutting medium is a peat soil: perlite: sand: vermiculite=1: 1: 1: the mixed-matrix of 1 (volume ratio).The Plastic Drum that fills the IAA50ppm solution for preparing is put in the branch bottom that trims, and soaking depth 3-4cm soaked 24 hours.The plugged ear that immersion treatment is crossed, on slotting machine, carry out cuttage, the cuttage degree of depth is 4-5cm, cutting density is seeding row spacing 6cm * 8cm, will spray water with watering can after inserting, and makes between plugged ear and the matrix fully contact, sprays 1000 times of liquid of carbendazim after the cuttage and carries out disinfection; Sunshade net and automatic sprayer are installed above the cuttage pond, are reduced intensity of illumination, increase air humidity, avoid the plugged ear dehydration.Previous month after cuttage, carry out comparatively strict moisture management, and the humidity of looking weather condition and cutting bed increases and decreases the number of times of spraying, the relative air humidity on daytime remains on more than 65～80%.After one month and the same management of the plant of other cuttage, regularly spray.The regular check situation of taking root, in the ordinary course of things, 10 days begin to occur callus after the cuttage, have new root to send after 20 days.Check the situation of taking root in late October, the rooting rate of this processing is 47.5%, and the number of on average taking root is 4, the long 6.4cm of average root, the rooting rate of this processing, take root several and root to grow equal ratio 1 low.
Embodiment 2
Late June, in the cold-resistant excellent strain that chooses, gather the current-year branch at tree crown sunny side middle part the morning of selection cloudy day or light rain, base portion band heel (the biennial branch of a very short part), length is 10～12cm.The spray of adopting back of giving birth to is then pruned, because the blade of the red fennel of lanceolata is longer, upper blade all cuts off half.Cuttage is on the slotting machine of sterilizing, and cutting medium is a peat soil: perlite: sand: vermiculite=1: 1: 1: the mixed-matrix of 1 (volume ratio).The Plastic Drum that fills the IBA50ppm solution for preparing is put in the branch bottom that trims, and soaking depth 3-4cm soaked 24 hours.The plugged ear that immersion treatment is crossed, on slotting machine, carry out cuttage, the cuttage degree of depth is 4～5cm, cutting density is seeding row spacing 6cm * 8cm, will spray water with watering can after inserting, and makes between plugged ear and the matrix fully contact, sprays 1000 times of liquid of carbendazim after the cuttage and carries out disinfection; Sunshade net and automatic sprayer are installed above the cuttage pond, are reduced intensity of illumination, increase air humidity, avoid the plugged ear dehydration.Previous month after cuttage, carry out comparatively strict moisture management, and the humidity of looking weather condition and cutting bed increases and decreases the number of times of spraying, the relative air humidity on daytime remains on more than 65～80%.After one month and the plant of other cuttage carry out same management, regularly spray.The regular check situation of taking root, in the ordinary course of things, 7 days begin to occur callus after the cuttage, have new root to send after 15 days, after 24 days, have 60% plugged ear to send new root.Check the situation of taking root in late October, the rooting rate of this processing is 70%, and the number of on average taking root is 7, the long 8.7cm of average root, and the branch axil place that survives has sprouting to send, and it is vigorous to grow.
Embodiment 3
Late June, in the cold-resistant excellent strain that chooses, gather the current-year branch at tree crown sunny side middle part the morning of selection cloudy day or light rain, base portion band heel (the biennial branch of a very short part), length is 10～12cm.The spray of adopting back of giving birth to is then pruned, because the blade of the red fennel of lanceolata is longer, upper blade all cuts off half.Cuttage is on the slotting machine of sterilizing, and cutting medium is a peat soil: perlite: sand: vermiculite=1: 1: 1: the mixed-matrix of 1 (volume ratio).The Plastic Drum that fills the IAA50ppm+IBA50ppm solution for preparing is put in the branch bottom that trims, and soaking depth 3-4cm soaked 24 hours.The plugged ear that immersion treatment is crossed, on slotting machine, carry out cuttage, the cuttage degree of depth is 4～5cm, cutting density is seeding row spacing 6cm * 8cm, will spray water with watering can after inserting, and makes between plugged ear and the matrix fully contact, sprays 1000 times of liquid of carbendazim after the cuttage and carries out disinfection; Sunshade net and automatic sprayer are installed above the cuttage pond, are reduced intensity of illumination, increase air humidity, avoid the plugged ear dehydration.Previous month after cuttage, carry out comparatively strict moisture management, and the humidity of looking weather condition and cutting bed increases and decreases the number of times of spraying, the relative air humidity on daytime remains on more than 65～80%.After one month and the same management of the plant of other cuttage, regularly spray.The regular check situation of taking root, in the ordinary course of things, 7 days begin to occur callus after the cuttage, have new root to send after 15 days, after 24 days, have 65% plugged ear to send new root.Check the situation of taking root in late October, the rooting rate of this processing is 80%, and the number of on average taking root is 8, the long 8.4cm of average root, and the branch axil place that survives has sprouting to send, and it is vigorous to grow.
Embodiment 4 contrasts
Late June, in the cold-resistant excellent strain that chooses, gather the current-year branch at tree crown sunny side middle part the morning of selection cloudy day or light rain, base portion band heel (the biennial branch of a very short part), length is 10～12cm.The spray of adopting back of giving birth to is then pruned, because the blade of the red fennel of lanceolata is longer, upper blade all cuts off half.Cuttage is on the slotting machine of sterilizing, and cutting medium is a peat soil: perlite: sand: vermiculite=1: 1: 1: the mixed-matrix of 1 (volume ratio).The Plastic Drum that fills clear water is put in the branch bottom that trims, and soaking depth 3～4cm soaked 24 hours.The plugged ear that immersion treatment is crossed, on slotting machine, carry out cuttage, the cuttage degree of depth is 4-5cm, cutting density is seeding row spacing 6cm * 8cm, will spray water with watering can after inserting, and makes between plugged ear and the matrix fully contact, sprays 1000 times of liquid of carbendazim after the cuttage and carries out disinfection; Sunshade net and automatic sprayer are installed above the cuttage pond, are reduced intensity of illumination, increase air humidity, avoid the plugged ear dehydration.Previous month after cuttage, carry out comparatively strict moisture management, and the humidity of looking weather condition and cutting bed increases and decreases the number of times of spraying, the relative air humidity on daytime remains on more than 65～80%.After one month and the same management of the plant of other cuttage, regularly spray.The regular check situation of taking root, in the ordinary course of things, 12 days begin to occur callus after the cuttage, have new root to send after 22 days, after 40 days, have 15% plugged ear to send new root.Check the situation of taking root in late October, the rooting rate of this processing is 30%, and the number of on average taking root is 3, and the long 6.4cm of average root has only the minority branch to send sprouting.It all is the poorest that this contrast survival rate and the indexs such as several of taking root are compared with HORMONE TREATMENT.
Claims (7)
1. the cold-resistant excellent strain cottage propagation technology of the red fennel of lanceolata is characterized in that carrying out according to following steps:
(1) sterilization of cutting bed, the configuration of cutting medium and sterilization.
(2) cutting time is the plum rain season of late June, gathers the current-year branch at tree crown sunny side middle part in the cold-resistant excellent strain of the red fennel of lanceolata, base portion band heel (the biennial branch of a very short part), and length is 10～12cm, top vane keeps half.
(3) with hormone made plugged ear is handled, the cuttage degree of depth 4～5cm, cutting density are seeding row spacing 6cm * 8cm, will spray water with watering can after inserting, and make between plugged ear and the matrix fully to contact; 1000 times of liquid of spray carbendazim carry out disinfection after the cuttage; Sunshade net and automatic sprayer are installed above the cuttage pond, are reduced intensity of illumination, increase air humidity, avoid the plugged ear dehydration.
(4) previous month after cuttage, carry out comparatively strict moisture management, and the humidity of looking weather condition and cutting bed increases and decreases the number of times of spraying, the relative air humidity on daytime remains on more than 65～80%.Change normal management after one month over to.
2. according to the cold-resistant excellent strain cottage propagation technology of the red fennel of right 1 described lanceolata, it is characterized in that cutting medium is a peat soil: perlite: sand: vermiculite=1: 1: 1: the mixed-matrix of 1 (volume ratio), the thickness in slotting machine are 30cm.
3. according to the cold-resistant excellent strain cottage propagation technology of the red fennel of right 1 described lanceolata, it is characterized in that working concentration be 0.5% potassium permanganate to nursery bed disinfection, carry out the sterilization of matrix with 800 times of liquid of thiophanate methyl of 50% again; Through 2～3 days, after the property of medicine volatilization, carry out cuttage again.
4. according to the cold-resistant excellent strain cottage propagation technology of the red fennel of right 1 described lanceolata, it is characterized in that early summer, plum rain season carried out the cuttage of spray, the time is in late June; Gather plugged ear from cold-resistant individual plant, gather the current-year branch at tree crown sunny side middle part, base portion band heel (the biennial branch of a very short part), length is 10～12cm, upper blade all cuts off half.
5. according to the cold-resistant excellent strain cottage propagation technology of the red fennel of right 1 described lanceolata, it is characterized in that plugged ear is handled with hormone IAA50ppm+IBA50ppm, the processing time is 20～24h.
6. according to the cold-resistant excellent strain cottage propagation technology of the red fennel of right 1 described lanceolata, it is characterized in that the cuttage degree of depth is 4～5cm, cutting density is seeding row spacing 6cm * 8cm, will spray water with watering can after inserting, and makes between plugged ear and the matrix fully to contact; 1000 times of liquid of spray carbendazim carry out disinfection after the cuttage; Sunshade net and automatic sprayer are installed above the cuttage pond, are reduced intensity of illumination, increase air humidity, avoid the plugged ear dehydration.
7. according to the cold-resistant excellent strain cottage propagation technology of the red fennel of right 1 described lanceolata, it is characterized in that the previous moon after cuttage, carry out comparatively strict moisture management, and the humidity of looking weather condition and cutting bed increases and decreases the number of times of spraying, the relative air humidity on daytime remains on more than 65～80%.Change normal management after one month over to.
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| CN102907244A (en) * | 2012-11-16 | 2013-02-06 | 广西壮族自治区林业科学研究院 | Short-scion cutting propagation method for star anise |
| CN102986441A (en) * | 2012-12-17 | 2013-03-27 | 苏州大学 | Cutting propagation method for ornamental plant star anise with lanceolate leaves |
| CN103609415A (en) * | 2013-11-26 | 2014-03-05 | 芜湖欧标农业发展有限公司 | Matrix for cultivating liana and method for using matrix for cutting propagation |
| CN105612996A (en) * | 2015-12-30 | 2016-06-01 | 浙江农林大学 | Chinese torreya cutting propagation method |
| CN105660128A (en) * | 2016-01-26 | 2016-06-15 | 和县聂兴圩蔬菜种植有限责任公司 | Fennel seedling cultivating method |
| CN105830574A (en) * | 2016-04-15 | 2016-08-10 | 中国林业科学研究院资源信息研究所 | Method for promoting recovery of native vegetation in tropical coast sandy land |
| CN107135925A (en) * | 2017-06-06 | 2017-09-08 | 上海市农业科学院 | A kind of mating system of Japanese thick grass seedling |
| CN113039947A (en) * | 2021-03-18 | 2021-06-29 | 金陵科技学院 | Salt-tolerant Euonymus persica twig cutting propagation method |
| CN114503884A (en) * | 2022-02-17 | 2022-05-17 | 中国林业科学研究院亚热带林业研究所 | Method for treating fennel seeds and sowing and seedling raising |
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| CN102577821A (en) * | 2012-03-17 | 2012-07-18 | 常熟市尚湖农业生态园有限公司 | Method for cuttage propagation of Rhodotypos scandens |
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| CN102907244A (en) * | 2012-11-16 | 2013-02-06 | 广西壮族自治区林业科学研究院 | Short-scion cutting propagation method for star anise |
| CN102986441A (en) * | 2012-12-17 | 2013-03-27 | 苏州大学 | Cutting propagation method for ornamental plant star anise with lanceolate leaves |
| CN103609415A (en) * | 2013-11-26 | 2014-03-05 | 芜湖欧标农业发展有限公司 | Matrix for cultivating liana and method for using matrix for cutting propagation |
| CN103609415B (en) * | 2013-11-26 | 2016-01-20 | 芜湖欧标农业发展有限公司 | A kind of matrix of cultivating climbing plant and the method using matrix cottage propagation |
| CN105612996A (en) * | 2015-12-30 | 2016-06-01 | 浙江农林大学 | Chinese torreya cutting propagation method |
| CN105612996B (en) * | 2015-12-30 | 2018-12-11 | 浙江农林大学 | A kind of Chinese torreya cuttage breeding method |
| CN105660128A (en) * | 2016-01-26 | 2016-06-15 | 和县聂兴圩蔬菜种植有限责任公司 | Fennel seedling cultivating method |
| CN105830574A (en) * | 2016-04-15 | 2016-08-10 | 中国林业科学研究院资源信息研究所 | Method for promoting recovery of native vegetation in tropical coast sandy land |
| CN107135925A (en) * | 2017-06-06 | 2017-09-08 | 上海市农业科学院 | A kind of mating system of Japanese thick grass seedling |
| CN113039947A (en) * | 2021-03-18 | 2021-06-29 | 金陵科技学院 | Salt-tolerant Euonymus persica twig cutting propagation method |
| CN114503884A (en) * | 2022-02-17 | 2022-05-17 | 中国林业科学研究院亚热带林业研究所 | Method for treating fennel seeds and sowing and seedling raising |
| CN114521454A (en) * | 2022-02-22 | 2022-05-24 | 中国林业科学研究院亚热带林业研究所 | Method for breeding and seedling raising of illicium simsii |
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