CN105612996A - Chinese torreya cutting propagation method - Google Patents
Chinese torreya cutting propagation method Download PDFInfo
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- CN105612996A CN105612996A CN201511011203.1A CN201511011203A CN105612996A CN 105612996 A CN105612996 A CN 105612996A CN 201511011203 A CN201511011203 A CN 201511011203A CN 105612996 A CN105612996 A CN 105612996A
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Abstract
The invention discloses a Chinese torreya cutting propagation method and belongs to the technical field of vegetative propagation of plants. The method is characterized by mainly comprising the following steps: 1, slotting machine preparation; 2, cutting slip collection; 3, cutting slip treatment and cutting; 4, cutting later-period management; 5, transplanting; 6, transplanting later-period management. By means of the technical scheme of the method, the cutting rooting rate of Chinese torreya reaches 96.67%, the number of lateral roots is large, and the root system is robust; statistics is conducted after 14 months passes since cutting seedlings are transplanted, the survival rate reaches 91.67%, the shoot growing amount is 4.53 cm, the root system is robust, the problems that rooting is not easy to achieve for Chinese torreya cutting, and the rooting cycle is long are solved, the method is simple, easy to operate and low in cost, effective and rapid vegetative propagation of Chinese torreya can be achieved, and great practical significance is achieved for accelerating popularization of excellent Chinese torreya genotypes, promoting Chinese torreya nursing, popularizing afforestation and conducting reasonable resource utilization.
Description
Technical field
The invention belongs to technical field of plant asexual propagation, it is specially a kind of method of Chinese torreya cottage propagation.
Background technology
Chinese torreya (Torreyagrandis ' Merrillii ') is a cultivated form in taxaceae (Taxaceae) Chinese torreya genus (TorreyaArn.) Chinese torreya (TorreyagrandisFort.exLindl), it it is the distinctive rare dry fruit of China, resource scarcity, life cycle is long, is the Quality and economy seeds class group collecting the multi-usages such as fruit is used, oil is used, medicinal, material is used, greening is viewed and admired, ecology. The nutritive value that Chinese torreya seed eats is very high, has abundant protein and various trace elements; Being processed into dry fruit can, as acat, also be rare traditional Chinese medicine simultaneously, and its nature and flavor are sweet flat, the effect be used as medicine and have and kill worm, anti-inflammatory moistening lung. In addition, the bark of Chinese torreya can obtain through refining industrial tannin extract. Its woody texture is straight and hardness is moderate, is the excellent material of shipbuilding, building, sleeper, furniture and technique engraving. Chinese torreya landscape effect is good, and the four seasons are evergreen, tree appearance is graceful; Ecological functions are strong, the resistance to the moon of seedlings or saplings, are suitable under sparse woods and plant, afforest original vegetation deterioration is few, are the fine tree species of Lower value stand transformation. Chinese torreya tree crown is dense, sylvan life fallen leaves thickness, and leaf easily rots, and water conservation, improvement soil are had significance.
The economic worth that current Chinese torreya seed is extremely high has promoted the development that Chinese torreya produces, planting scale increases considerably, the demand of nursery stock is increased day by day, but the many grafting breedings of Chinese torreya, only making stock with Chinese torreya at least to be cultivated 2 years, conventional 2+2(2 stock is cultivated+2 years Grafts and is cultivated) grafting nursery stock, the production cycle is relatively long, and have the trend cultivating tree transplanted at present, and cottage propagation not yet widespread use on producing.
In recent years, due to the economic worth that Chinese torreya is extremely high, the research report about Chinese torreya is quite a few, mainly lays particular emphasis on cytology and histology, molecule marker, composition analysis, grafting breeding technology, cultivates and foster technology, tissue culture etc. Also relevant report is had with regard to Chinese torreya cuttage technique, as: patent documentation (publication number: CN104838829A) discloses the Clonal regeneration method of the edible Chinese torreya of a kind of high-quality, it directly makes cuttings with choosing branch, seedling is cultivated in cuttings cuttage on seedbed, eliminate the process of tradition Chinese torreya grafting vernalization breeding grafting, greatly reduce seedling cost, and shorten the result time of nursery stock. Carry out Chinese torreya 1a raw cuttage Chen Shumao (2001) mid-April, next year surviving rate 0%; Late June cuttage, next year surviving rate 65%. Guo Weihua (2002) on July, the middle ten days carry out cuttage, use 1000mg L-1ABT6 root-inducing powder (GGR6) processes, and survival rate 92% then, next year possesses rate 83%. Wan little Jin etc. (2006) study discovery, Chinese torreya 1a life in spring branch cutting, surviving rate 62%; Summer is with giving birth to semi-lignified branch cuttage, base end 0.05%ABT1 root-inducing powder or naphthylacetic acid (NAA) 1000mg L then-1Solution dips in 10s, and about 55d is taken root, seedling rate 80%. These tests obtain certain PRELIMINARY RESULTS; Chinese torreya cuttage there is certain positively effect; but Chinese torreya cuttage root-taking difficulty could not be solved preferably as the technical barrier of this area always; in fact because how undeveloped Chinese torreya cuttage seeding root system is; especially lacking healthy and strong side root, cuttage survival rate can cause one " illusion " usually, and namely branch survives on the surface; substantially do not take root or seldom take root in branch bottom, also very easily dead when adopting this kind of cuttage seeding to transplant.
Summary of the invention
For the technological deficiency existed in prior art, it is an object of the invention to provide a kind of method of Chinese torreya cottage propagation, solve the technical problem that cuttage root-taking is difficult, transplanting survival rate is low.
The technical solution adopted in the present invention is as follows:
A kind of Chinese torreya cuttage breeding method, it is characterised in that: mainly comprise the steps:
1) slotting machine prepares: builds slotting machine in greenhouse, lays the sunshade net of transmittance 50% above cutting bed, filling cutting medium in slotting machine;
Slotting machine is the foam chute composition of 0.3m (W) �� 1.1m (L) �� 0.2m (H), fixes with iron wire, and bottom is with drainage tray and water vent; 0.03% potassium permanganate solution is sprayed on foam chute surface and coating film sealing sterilization 14d before cuttage;
In slotting machine, the matrix of filling is the mixed-matrix of peat, perlite and vermiculite, and volume proportion is: peat: perlite: vermiculite=1:1:1; First spread, bottom slotting machine, the haydite layer of displacement that one layer of thick radius of 3cm is 1-2cm during filling matrix; Before cuttage, 2d sprays 0.125% derosal (25% effective content) solution, matrix is sterilized;
2) cuttings collection: the branch (hereinafter referred to as band heel branch) gathering raw semi-lignified branch and the raw branch section of the long 2a of base strip one section of 3cm then summer from the female strain of 10-12a raw Chinese torreya makes cuttings, otch is wrapped up immediately with wet towel, to prevent cuttings dehydration, oxidation, require robust growth, without the branch of disease and pest and physical abuse; Remove the blade in the raw branch section of 2a; Whole cuttings is immersed in 0.125% carbendazim solution to sterilize about 2min, is placed on shady and cool place for subsequent use after draining; The band heel branch adopted is the current-year branch retaining 3 tool terminal buds in the raw branch section of 2a;
As preferably, cuttings specification is preferably rugosity 0.2-0.3cm, length 13-15cm, wherein long 14cm, thick 0.27cm effect is best;
3) cuttings process and cuttage: with sharp cutter truncation cuttings base portion before cuttage, it is noted that keep smooth cut smooth, use 2.5mg L-1After the special taking root liquid immersion cuttings base portion 15s of mark allusion quotation 3721 cuttage, cuttage is in the matrix of slotting machine, and the cuttage degree of depth is for being placed in matrix by raw for 2a branch section; Water immediately after cuttage, ensure that cuttings base portion fully contacts with matrix;
4) cuttage final-period management: mainly comprise water management, fertilising and the prevention and control of plant diseases, pest control;
Water management method is as follows: the cuttage initial stage, utilizes auto spraying equipment and sunshade net that cuttings is carried out moisturizing temperature control, and spray intervals is that spraying 30s stops 5min, and working time is 8:30-17:00, keeps slotting machine humidity 70-90%, temperature less than 33 DEG C; Cuttings Callus formation and flourishing after, adjustment spray intervals stops 20min for spraying 30s, and working time is 8:30-17:00;
Fertilising is as follows with pest control method: spray 0.125% carbendazim solution every 10-15d, with 0.01% zinc manganese ethylenebisdithiocarbamate wheel use, in case producing resistance; At Callus formation and flourishing after, spray 0.2% potassium primary phosphate and 0.15% urea mixed solution as foliage fertilizer, the interval phase is 10-15d;
5) transplant: cuttage seeding was transplanted in after cuttage 5 months; Matrix is peat: perlite: vermiculite=1:1:1(V/V/V), container is non-woven fabrics Seedling bag; As preferably, preferably carry out being completely cured point process before transplanting;
6) final-period management is transplanted: executing green manure (OSMOCOTE) No. 5 controls difficult to understand March in next year after transplanting and release fertilizer (nitrogen phosphoris and potassium fertilizer ratio is 14-13-13), every basin is about 2g.
Adopting above technical scheme, Chinese torreya cutting plantation reaches 96.67%, and side radical is many, well developed root system; Cuttage seeding is transplanted and is added up for latter 14 months, survival rate 91.67%, shoot length 4.53cm, well developed root system. The invention solves that Chinese torreya cuttage is not easily taken root, the problem of cycle length of taking root, method is simple, cost is low, Chinese torreya vegetative propagation fast and effectively can be realized, promote accelerating Chinese torreya superior genotypes, promote that its child care and the Appropriate application of afforestation popularization and resource have extremely important realistic meaning.
Embodiment
Below in conjunction with specific embodiment, the present invention is further detailed explanation. Mentioned concentration does not explain, and is the concentration method for expressing of the routine of this area, i.e. mass percentage concentration.
Embodiment 1
Chinese torreya cuttage mainly comprises the steps:
1) slotting machine prepares: cuttage place is positioned at the greenhouse that Linan city of Zhejiang Province (north latitude 29 �� of 56'-30 �� of 23', east longitude 118 �� of 51'-119 �� of 52') Zhejiang agricultural university subtropics forest cultivates National Key Laboratory base. Greenhouse cutting bed is built 1.8m height brandreth, lays the sunshade net of transmittance about 50%, be equipped with auto spraying equipment. Slotting machine is the foam chute composition of 0.3m (W) �� 1.1m (L) �� 0.2m (H), fixes with iron wire, and bottom is with drainage tray and water vent. 0.03% potassium permanganate solution is sprayed and coating film sealing sterilization 14d on foam chute surface;
2) cutting medium: matrix is peat (0-10mm, Denmark Pindstrup), vermiculite (5-8mm), perlite (2-4mm), by peat: perlite: vermiculite=1:1:1(V/V/V) mixing match. First spread, bottom slotting machine, the haydite layer of displacement that one layer of thick radius of 3cm is 1-2cm during filling matrix. Before cuttage, 2d sprays 0.125% derosal (25% effective content) solution, matrix is sterilized. The sterilization of slotting machine and matrix can reduce the generation of cuttings disease and pest, ensures the surviving rate of Chinese torreya cuttage;
3) cuttings collection and making: in morning early July or the at dusk Chinese torreya cultivation base in agricultural campus, Zhejiang, from the female strain of 10-12a raw Chinese torreya, gather band heel branch do cuttings and (the raw branch section of 2a retains the current-year branch of 3 tool terminal buds, long about 14cm, thick about 0.27cm), require robust growth, without the branch of disease and pest and physical abuse. The process of cuttings completes in 2h after acquisition as far as possible, and wraps up cuttings with wet towel at any time in treating processes, to prevent cuttings dehydration, oxidation. Remove heel with lower blade. Whole cuttings is immersed in 0.125% derosal (25% effective content) solution to sterilize about 2min, is placed on shady and cool place for subsequent use after draining;
4) cuttage: with sharp cutter truncation cuttings base portion before cuttage, it is noted that keeping smooth cut smooth, it is more short more good that otch exposes the time in atmosphere, uses 2.5mg L-1The mark allusion quotation 3721 special taking root liquid of cuttage (Zhengzhou Biao Dian Science and Technology Development Co., Ltd.) soaks cuttage after cuttings base portion 15s. The cuttage degree of depth should not be excessively dark, about 3cm, spacing 6 �� 6cm, and compresses the matrix around compacting cuttings, waters immediately after cuttage, ensures that cuttings base portion fully contacts with matrix;
5) cuttage final-period management:
Water management: cuttage initial stage, utilizes auto spraying equipment and sunshade net that cuttings is carried out moisturizing temperature control, and spray intervals is that spraying 30s stops 5min, and working time is 8:30-17:00, keeps slotting machine humidity 70-90%, temperature less than 33 DEG C. Cuttings Callus formation and after flourishing (about 1 month), adjustment spray intervals stops 20min for spraying 30s, and working time is 8:30-17:00;
Fertilising and the prevention and control of plant diseases, pest control: spray 0.125% derosal (25% effective content) solution every 10-15d, with 0.01% zinc manganese ethylenebisdithiocarbamate wheel use, in case producing resistance. Plum rain season to be strengthened control, ventilates in time, prevents cuttings disease and pest from occurring. At Callus formation and after flourishing (about 1 month), spraying 0.2% potassium primary phosphate and 0.15% urea mixed solution as foliage fertilizer, the interval phase is 10-15d, has and improves cuttage seeding resistance, the effect of growth promoting effects;
6) transplant: after cuttage, cuttage seeding is transplanted by 5 months (then December). Matrix is peat: perlite: vermiculite=1:1:1(V/V/V), container is 7cm �� 15cm non-woven fabrics Seedling bag (bag is planted by U.S.A). Point process of being completely cured is carried out before transplanting;
7) final-period management is transplanted: execute green manure (OSMOCOTE) No. 5 controls difficult to understand March in next year after transplanting and release fertilizer (nitrogen phosphoris and potassium fertilizer ratio is 14-13-13).
Adopting the program, Chinese torreya cutting plantation reaches 96.67%, and side radical is many, well developed root system; Cuttage seeding is transplanted and is added up for latter 14 months, survival rate 91.67%, shoot length 4.53cm, well developed root system.
Embodiment 2
1 test ground overview
Test ground is positioned at greenhouse and the laboratory that Linan city of Zhejiang Province (north latitude 29 �� of 56'-30 �� of 23', east longitude 118 �� of 51'-119 �� of 52') Zhejiang agricultural university subtropics forest cultivates National Key Laboratory base. Greenhouse cutting bed is built 1.8m height brandreth, lays the sunshade net of transmittance about 50%, be equipped with auto spraying equipment.
2 materials and methods
2.1 test materialss and process
Morning or at dusk the Chinese torreya cultivation base in agricultural campus, Zhejiang, gather raw robust growth then, without the branch (hereinafter referred to as common branch) of disease and pest and physical abuse from the female strain of 10-12a raw Chinese torreya. The process of cuttings completes in 2h after acquisition as far as possible, and wraps up cuttings with wet towel at any time in treating processes, to prevent cuttings dehydration, oxidation. Cuttings rugosity 0.2-0.4cm, length 13-15cm, lignifying degree is close, and removes the 1/3 of the total blade in base portion. Whole cuttings is immersed in 0.125% derosal (25% effective content) solution to sterilize about 2min, is placed on shady and cool place for subsequent use after draining. The front sharp cutter of cuttage is cut sth. askew cuttings base portion, it is noted that keeping smooth cut smooth, it is more short more good that otch exposes the time in atmosphere, and carries out exogeneous growth conditioning agent process. The cuttage degree of depth is cuttings total length 1/3, is generally 5cm, spacing 4 �� 5cm, and compresses the matrix around compacting cuttings, ensures that cuttings base portion fully contacts with matrix.
2.2 test method
2.2.1 cuttage season is on the impact of Chinese torreya cuttage root-taking
Four seasons cuttage, cuttage in spring carried out on April 2nd, 2015, and cuttage in summer carried out on July 8th, 2014, and cuttage in autumn carried out on October 6th, 2014, and cuttage in winter carried out on January 30th, 2015. Adopting current-year branch, 300mg L is used in unification-1GGR6(have another name called two Ji Er root-inducing powder; Ai Bidi bio tech ltd, Beijing produces) soak 20min. Each season is a process, is often treated to 30 strains, repeats 3 times.
2.2.2 the process of exogeneous growth conditioning agent is on the impact of Chinese torreya cuttage
Gather Chinese torreya semi-wooden scion summer and carry out cuttage, with (1) GGR6; (2) ��-naphthaleneacetic acid (��-Naphthaleneacetic, NAA); (3) indolebutyric acid (Indole-3-butyricacid, IBA) 3 kinds of exogeneous growth conditioning agents (A) process, and often kind of hormone adopts 3 kinds of concentration (B), i.e. (1) 100mg L-1; (2) 200mg L-1; (3) 500mg L-1. Cuttings all processes 20min, soaks cuttings 20min as comparison taking (CK) clear water. Having 10 process combinations, often process combination 30 strains, repeat 3 times.
2.2.3 the selection of cuttings and process are on the impact of Chinese torreya cuttage
Gather band heel branch (with embodiment 1) summer respectively, raw then semi-lignified branch (long about 8.4cm, slightly about 0.24cm) from Chinese torreya tree crown top make cuttings, carry out cuttage in greenhouse; Gather band heel branch (long about 11.3cm, slightly about 0.22cm), then raw common semi-lignified branch summer respectively and make cuttings (long about 10.5cm, slightly about 0.27cm), carry out cuttage in artificial climate indoor. All cuttings 2.5mg L-1The mark allusion quotation 3721 special taking root liquid of cuttage (Zhengzhou Biao Dian Science and Technology Development Co., Ltd.) soaks cuttage after cuttings base portion 15s. Often it is treated to 10 strains, repeats for 3 times.
2.2.4 different substrates is on the impact of Chinese torreya cuttage root-taking
Gather the hard branch of Chinese torreya then autumn and carry out cuttage, basically identical (the long 14-15cm of cuttings specification, thick 0.24-0.30cm), there are peat (0-10mm, Denmark Pindstrup), vermiculite (5-8mm), perlite (2-4mm), rice hull carbon (Dalian ninety percent) for test matrix. Totally five kinds of proportionings, i.e. (A) peat: perlite: vermiculite=1:1:1(V/V/V, lower same); (B) peat: perlite: vermiculite=1:2:1; (C) peat: perlite: vermiculite=1:4:1; (D) peat: perlite: vermiculite=1:8:1; (E) peat: perlite: vermiculite: rice hull carbon=1:3:1:1. Matrix was prepared on October 5th, 2014, then carried out cuttage. During cuttage, management is consistent, as carried out spraying etc. simultaneously. Often kind of substrate composition is regarded as 1 process, is often treated to 30 strains, repeat for 3 times. Agricultural Environmental Monitoring instrument (precision is 3% for TNHY-D-16, instrument company limited of Hangzhou Top) is used to carry out water supply in media assay during about 2:00 in 3.5 months afternoons after substrate preparation cuttage.
2.2.5 different lower cut process is on the impact of Chinese torreya cuttage root-taking
Gathering spray summer, respectively cuttings base portion is carried out truncation, chamfering process, 300mg L is used in unification-1GGR6 soak cuttage after 20min.
2.2.6 different sources branch cultivates the impact on Chinese torreya growth of cuttage seedlings
By (A) band heel branch, (B) tree crown top branch, (C) bottom branch in tree crown, (D) common branch cutting is bred 5 months and is grown more consistent cuttage seeding and do not do any process and transplant, transplanting medium and container are with embodiment 1, to also namely starting from cuttage after 9 months, the common cultivation nursery stock of 14 months carries out Root morphology, biomass, increment measure, and add up the storage rate of nursery stock. Every seedling randomly draws 1 strain, repeats for 3 times.
2.3 rooting inhibitors measures and statistics
After cuttage, carrying out taking root state observation every 7-10d, record callus goes out current, adventive root and goes out current, and often 3 strains are got in process every time, carefully plants back after observing. Taking root in major part process, main root extends and carries out statistics of taking root, investigation statistics rooting rate, callus rate, mortality ratio when there is side root. Often process select 5 representative strains to add up the indefinite radical of each cuttings, namely main root number, side radical, 3 times repeat, totally 15 strain; Measuring main root length, side root length with ruler, and calculate Rooting index (rooting rate �� average radical �� average root length), average root length is average total root length (cm) of single strain.
Root morphology is observed: get the cuttage transplanted seedling that each process cultivation time is 14 months, cuttings root system is carried out digital scanning (EpsonperfectionV700photo, Japan) to obtain complete root system image, then with supporting root system analytical system software WinRHIZO_ProV2007b(RegentInstrumentInc.Canada) analyze Root morphology, obtain the parameters such as root length, root surface area, root volume.
Biomass estimation measures based on the dry biomass after oven dry (80 DEG C, 72h is to constant weight), and often process selects 5 representative strains to measure. Root/shoot ratio=Underground biomass dry weight/over-ground part biomass dry weight.
2.4 data statisticss and analysis
Utilizing Excel2003 surveyed data to be arranged, data SPSS18.0 software carries out statistical study. Percentage ratio and enumeration data carry out the conversion of square root inverse sine and square root conversion respectively, then carry out variance analysis; With duncan's new multiple range method (Duncan method), the result of variance analysis significant difference is carried out multiple comparisons.
3 results and analysis
3.1 cuttage seasons are on the impact of Chinese torreya cuttage root-taking
The cuttage of the current-year branch four seasons starts statistics of taking root after growing side root. Spring is 163d after cuttage, and summer is 170d after cuttage, and autumn is 339d after cuttage, and winter, 225d carried out statistics of taking root after cuttage. Observation shows, Chinese torreya carries out cuttage four seasons can take root (table 1). Spring and summer carry out cuttage, and the cuttage rooting time is less than fall and winter. The cuttings root in spring and summer extends the phase and is respectively 83d, 65d after cuttage, and at 168d, 145d, autumn, winter occur that root extends (table 1) respectively.
After cuttings cuttage or survive, or dead, the cuttings survived has again to be taken root and only forms callus and do not form the situation of root, and the percentage sum of three is 100%. There is the multiple comparisons done on the basis of significant difference to show at one-way analysis of variance, spring and summer cutting plantation be significantly higher than fall and winter (P < 0.05), spring, rooting rate was 31.67%, summer is 31.11%, respectively higher than fall and winter by 8.34%, 23.34% and 7.78%, 22.78%(table 2). The cuttings statistics only forming callus and do not take root is callus rate. Cuttage in summer cuttings callus rate is significantly higher than season in winter in spring and autumn three (P < 0.05), reaches 61.11%, respectively higher by 56.66% than other three seasons, 45.55%, 56.11%(table 2). Surviving rate is rooting rate and callus rate sum, summer (92.22%) > autumn (38.89) > spring (36.67%) > winter (13.33%). Mortality ratio is winter (86.67%) then > spring (63.33%) > autumn (61.11%) > summer (7.78%), spring and autumn is close, minimum with the mortality ratio of cuttage in summer.
The main root number of each cuttings of four seasons cuttage is without obvious difference, but fall and winter cuttage side radical is significantly higher than spring and summer (table 2). Spring and cuttage in autumn, its Rooting index is significantly higher than winter (P < 0.05) (table 2). But from the cultivation time, cuttage in spring less than autumn, autumn cuttage seeding cultivate the time than spring more 176d(tables 2). From over-ground part dry mass, spring to winter, cuttings over-ground part dry mass increases progressively gradually, and winter is the highest, reaches 1.14g, and spring is minimum, is 0.62g, and season in autumn in summer two is respectively 0.87g, 0.99g. Cuttage in spring, its root/shoot ratio is significantly higher than other the third quaters, reaches 0.14, respectively than improve 133.33% season autumn and winter in summer three, 40%, 180%(P < 0.05).
It thus is seen that four seasons cuttage, cuttage in summer is better, and surviving rate is significantly higher than other seasons, and mortality ratio is low, and rootage duration is shorter, and rooting rate is higher.
3.2 exogeneous growth conditioning agent process are on the impact of Chinese torreya cuttage root-taking
Two-way analysis of variance result shows, Chinese torreya cuttage root-taking in summer is all had remarkably influenced (P < 0.05) (table 3) by exogeneous growth conditioning agent kind (A), concentration (B). The main root number of rooting rate, callus rate, each cuttings, side radical, Rooting index, root dry mass, root/shoot ratio are had pole remarkably influenced (P < 0.01) (table 3) by A. The side radical of rooting rate, callus rate, each cuttings is had pole remarkably influenced (P < 0.01) (table 3) by B. The rooting rate of cuttings, callus rate, over-ground part dry mass, root/shoot ratio are had pole remarkably influenced (P < 0.01) by the interaction (A �� B) of dual factors, the main root number of each cuttings is had remarkably influenced (P < 0.05), and all the other each indexs then do not make significant difference (table 3).
The multiple comparisons that Chinese torreya cuttage rooting affects is shown by exogeneous growth conditioning agent kind, rooting rate NAA > IBA > GGR6(P < 0.05) (table 4), callus rate GGR6 > IBA > NAA, is inversely proportional to rooting rate. The main root number of each cuttings, side radical, Rooting index NAA > IBA > GGR6. Root dry mass, root/shoot ratio NAA > IBA > GGR6, wherein NAA and IBA close (P < 0.05) (table 4). Therefore, Chinese torreya cuttage in summer exogeneous growth conditioning agent kind selects NAA better.
The multiple comparisons that Chinese torreya cuttage rooting affects is shown by exogeneous growth modifier concentration, rooting rate and callus rate 100mg L-1With 200mg L-1Process is significantly higher than 500mg L-1Process (P < 0.05) (table 5). The side radical of each cuttings is 500 > 200 > 100mg L then-1Process (P < 0.05). Root/shoot ratio 100mg L-1Process is significantly higher than 500mg L-1Process (P < 0.05), all the other each indexs are difference remarkable (P < 0.05) (table 5) then. The exogeneous growth conditioning agent rooting rate of lower concentration and root/shoot ratio height, callus rate is also high, but side radical is significantly lower than the exogeneous growth conditioning agent of high density. Therefore, Chinese torreya cuttage in summer, exogeneous growth modifier concentration selects 100mg L-1Or 200mg L-1Process is better.
Comprehensive analysis, 100mg L-1Or 200mg L-1NAA to process Chinese torreya cuttings better, rooting rate is respectively 64.44%, 55.56%, compares (CK) than clear water and exceeds 25.18%, 16.3% respectively, the main root number of each cuttings, side radical, Rooting index higher (table 6).
Selecting with process the impact of Chinese torreya cuttage of 3.3 cuttings
One-way analysis of variance result shows, Chinese torreya cuttage root-taking in summer is had remarkable effect (P < 0.05) (table 7) under greenhouse experiment by dissimilar cuttings, band heel branch does cuttings cuttage, the side radical of each cuttings, Rooting index, ground and root dry mass are significantly higher than the branch from Chinese torreya tree crown top (P < 0.05), and then between different cuttings, difference is not significantly (P < 0.05) (table 7) for all the other each indexs such as rooting rate, mortality ratio. Call in person branch cutting better in greenhouse with band, and its rooting rate is 96.67%, and side radical is 13.83, and root dry mass height, root growth is better.
3.4 different substrates are on the impact of Chinese torreya cuttage root-taking
One-way analysis of variance shows, the water content difference of different ratio matrix remarkable (P < 0.05) (table 8), A > E > B > C > D; A to D is along with the increase of perlite ratio, and water-permeable strengthens gradually, and water supply in media content reduces gradually; A water supply in media content is the highest, is that 19.53%, D is minimum, is 4.83%.
One-way analysis of variance also shows simultaneously, different ratio matrix on Chinese torreya cutting plantation, callus rate, take out tip rate, cuttings side radical, Rooting index, root dry mass, root/shoot ratio affect significant difference (P < 0.05) (table 9). Rooting rate process A is significantly higher than other process (P < 0.05), A > D > C > B > E, and wherein C and D is close; Callus rate A and C is significantly higher than other process (P < 0.05), C > A > B > D > E, and wherein A and C is close; Surviving rate A > C > D > B > E; Mortality ratio is then contrary with surviving rate; Taking out tip rate A and be significantly higher than other process (P < 0.05), A > C > D > B > E, wherein C and D is close. Side radical D is significantly higher than other process, and between the main root number of each cuttings processes, difference is not remarkable. Rooting index A and D is significantly higher than B and C(P < 0.05). Root dry mass and root/shoot ratio process D are significantly higher than other process, and difference remarkable (P < 0.05) between the process of over-ground part dry mass.
Therefore, A is best in process, its rooting rate, surviving rate, take out tip rate, side radical, Rooting index higher, and process D Rooting index and root/shoot ratio higher, process E rooting rate and surviving rate are minimum.
The process test of 3.5 cutting base portions
One-way analysis of variance shows, cuttings base portion's truncation and cutting sth. askew, the main root number of its rooting rate, callus rate, each cuttings, side radical, Rooting index, root dry mass, root/shoot ratio process between significant difference (P < 0.05) (table 10). Truncation cutting plantation is 61.67%, improves 30.56% than cutting sth. askew, and the side radical on the main root number of each cuttings, each main root, Rooting index improves 97.42%, 106.57%, 1049.50% respectively, and surviving rate is cut sth. askew higher than truncation. Truncation is compared and is cut sth. askew, and root/shoot ratio improves 50%. Therefore, the truncation of base portion is better than cutting sth. askew, and the main root number of its rooting rate, each cuttings, side radical, Rooting index, root/shoot ratio significantly improve.
3.6 different sources branches cultivate the impact on Chinese torreya growth of cuttage seedlings
Chinese torreya cuttage in summer finds, the branch rooting rate of three types and quality of rooting are higher, is respectively band heel branch, tree crown top, middle bottom branch. Therefore transplant after 9 months, observe these process cuttage seeding root systems and growing state tool is of great significance.
The cuttage seeding that Chinese torreya different sources branch is cultivated, cultivates after 14 months significant difference (P < 0.05) (table 11) between root system characteristic dissimilar seedling. A class seedling is except root average diameter, and all the other root system characteristic index are significantly higher than other process; Root system surface-area, the root system volume of B and D class seedling are significantly higher than C; Root average diameter B is significantly higher than C; Root system overall length, tip of a root number be difference remarkable (P < 0.05) then. Therefore, A root traits is best, B and D takes second place, and processes worst.
Sprout several A significantly lower than other types, storage rate and take out tip rate difference not significantly (P < 0.05). Increment D is significantly higher than other types; Being significantly higher than other types with root dry mass A on the ground, C is significantly less than B and D; Root/shoot ratio A and C is significantly higher than B and D(P < 0.05).
Comprehensive analysis, A ground, root biomass and root/shoot ratio are the highest, and root traits is good, but the number that sprouts is few, takes out tip rate low; B root proterties is taken second place; D is increment height on the ground, and ground biomass is also high, but root traits is poor, and root/shoot ratio is minimum; C on the ground, root biomass minimum, root traits is poor, but root/shoot ratio height.
Claims (8)
1. a Chinese torreya cuttage breeding method, it is characterised in that: mainly comprise the steps:
1) slotting machine prepares: builds slotting machine in greenhouse, lays the sunshade net of transmittance 50% above cutting bed, filling cutting medium in slotting machine;
2) cuttings collection: the branch gathering raw semi-lignified branch and the raw branch section of the long 2a of base strip one section of 3cm then summer from the female strain of 10-12a raw Chinese torreya makes cuttings, otch is wrapped up immediately with wet towel, to prevent cuttings dehydration, oxidation, it is desired to robust growth, without the branch of disease and pest and physical abuse; Remove the blade in the raw branch section of 2a; Whole cuttings is immersed in 0.125% carbendazim solution to sterilize about 2min, is placed on shady and cool place for subsequent use after draining;
3) cuttings process and cuttage: with sharp cutter truncation cuttings base portion before cuttage, it is noted that keep smooth cut smooth, use 2.5mg L-1After the special taking root liquid immersion cuttings base portion 15s of mark allusion quotation 3721 cuttage, cuttage is in the matrix of slotting machine, and the cuttage degree of depth is for being placed in matrix by raw for 2a branch section; Water immediately after cuttage, ensure that cuttings base portion fully contacts with matrix;
4) cuttage final-period management: mainly comprise water management, fertilising and the prevention and control of plant diseases, pest control;
5) transplant: cuttage seeding was transplanted in after cuttage 5 months; Matrix is peat: perlite: vermiculite=1:1:1(V/V/V), container is non-woven fabrics Seedling bag;
6) final-period management is transplanted: executing green manure No. 5 controls difficult to understand March in next year after transplanting and release fertilizer, every basin is about 2g.
2. the method for a kind of Chinese torreya cottage propagation according to claim 1, it is characterized in that: the slotting machine in described step 1) is the foam chute composition of 0.3m (W) �� 1.1m (L) �� 0.2m (H), fixing with iron wire, bottom is with drainage tray and water vent; 0.03% potassium permanganate solution is sprayed on foam chute surface and coating film sealing sterilization 14d before cuttage.
3. a kind of Chinese torreya cuttage breeding method according to claim 1, it is characterised in that: in the slotting machine in described step 1), the matrix of filling is the mixed-matrix of peat, perlite and vermiculite, and volume proportion is: peat: perlite: vermiculite=1:1:1; First spread, bottom slotting machine, the haydite layer of displacement that one layer of thick radius of 3cm is 1-2cm during filling matrix; Before cuttage, 2d sprays 0.125% carbendazim solution, matrix is sterilized.
4. a kind of Chinese torreya cuttage breeding method according to claim 1, it is characterised in that: described step 2) in the long 13-15cm of cuttings, thick 0.2-0.3cm, and the raw branch section of 2a retains the current-year branch of 3 tool terminal buds.
5. a kind of Chinese torreya cuttage breeding method according to claim 1, it is characterised in that: described step 2) in the long 14cm of cuttings, thick 0.27cm, and the raw branch section of 2a retains the current-year branch of 3 tool terminal buds.
6. a kind of Chinese torreya cuttage breeding method according to claim 1, it is characterized in that: in described step 4), water management method is as follows: the cuttage initial stage, utilize auto spraying equipment and sunshade net that cuttings is carried out moisturizing temperature control, spray intervals is that spraying 30s stops 5min, working time is 8:30-17:00, keep slotting machine humidity 70-90%, temperature less than 33 DEG C; Cuttings Callus formation and flourishing after, adjustment spray intervals stops 20min for spraying 30s, and working time is 8:30-17:00.
7. a kind of Chinese torreya cuttage breeding method according to claim 1, it is characterised in that: in described step 4), fertilising is as follows with pest control method: spray 0.125% carbendazim solution every 10-15d, with 0.01% zinc manganese ethylenebisdithiocarbamate wheel use, in case producing resistance; At Callus formation and flourishing after, spray 0.2% potassium primary phosphate and 0.15% urea mixed solution as foliage fertilizer, the interval phase is 10-15d.
8. a kind of Chinese torreya cuttage breeding method according to claim 1, it is characterised in that: described step 5) carries out point process of being completely cured before transplanting.
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