CN106212236A - A kind of Rhododendron fortuneilindl． Callus formation and technology taken root of promoting - Google Patents
A kind of Rhododendron fortuneilindl． Callus formation and technology taken root of promoting Download PDFInfo
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- CN106212236A CN106212236A CN201610625262.6A CN201610625262A CN106212236A CN 106212236 A CN106212236 A CN 106212236A CN 201610625262 A CN201610625262 A CN 201610625262A CN 106212236 A CN106212236 A CN 106212236A
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- cutting
- root
- seedbed
- promoting
- callus formation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
Abstract
The invention discloses a kind of Rhododendron fortuneilindl． Callus formation and technology taken root of promoting, step is: seedbed laying, cutting collection, cutting making, root induction, cuttage and management.Period employing ground hot line of taking root carries out seedbed and heats, and automatic intermittent spraying system water supplement is humidified, and temperature controls at 15.0 20.0 DEG C, relative humidity 85.0 95.0%, and every 14 d spraying fungicide rots to reduce cutting for 1 time.Present invention efficiently solves Rhododendron fortuneilindl． cottage propagation take root difficulty, rooting rate is low, the problems such as number is few of taking root, rooting rate is up to 73.67%, and well developed root system, for a kind of effective propagation method of Rhododendron fortuneilindl．, to accelerating, China's Folium Rhododendri Simsii industrialized development is significant.
Description
Technical field
The invention belongs to plant cuttage propagation technical field, be specifically related to a kind of Rhododendron fortuneilindl． Callus formation of promoting
Cutting wound healing processing method and root induction technology.
Background technology
Cottage propagation not only can keep maternal merit, nursery stock size uniformly, blossom and bear fruit early, and to facility condition
Require simple, low cost, easily promote, be the most widely used in lobule fallen leaves and the industry of half evergreen class Cuculus polioephalus (Azalea)
Change nursery.Restricted by genetic factor, originate in high mountain mountain region Subgenus Hymenanthes (Rhododendron Subgenus
Hymenanthes) take root difficulty, planting percent of Cuculus polioephalus is low, mainly passes through seeding and seedling raising in China.Although seed propagation breeding coefficient
Greatly, but growth of seedling is slow, blooming cycle is long, and between Cuculus polioephalus kind, natural hybridization probability is big, it is difficult to guarantee gene purity.
Rhododendron fortuneilindl． (Rhododendron fortunei) it is subordinate to Ericaceae (Ericaceae) Rhododendron
(Rhododendron) Subgenus Hymenanthes, originate in ridge Yang Chu or the sylvan life of Yangtze River in China basin height above sea level 620-2000 m.For
Evergreen shrubs is to dungarunga, and high 3-12 m, blade is big, heavy leather matter, bottle green, and the raw total shape umbel in top has colored 6-12, flower
Pink, savory, the 4-5 month is open.This species original name " temmoku Cuculus polioephalus ", because the flower quantity in its inflorescence is many, flower big, contains
If bright Nanjing brocade when opening, so descendant is renamed as " Rhododendron fortuneilindl． ", has good for a kind of flower, leaf and strain shape and have the excellent of fragrance
Ornamental plant, and growth is fast, florescence length, cultivation adaptability are relatively strong, is also the important parent of alpine rose rearing new variety, has
Huge Commercial Prospect, but the report of the most rarely seen cottage propagation.Therefore, it is necessary to set up cuttage and seedling culture method, for Nanjing brocade
The industrialization of Cuculus polioephalus provides technical support.
Summary of the invention
The technical problem to be solved in the present invention is that great Ye evergreen class Cuculus polioephalus cuttage root-taking is difficult, quantity of taking root is few, survival rate
The problem such as low.In view of the above problems, it is an object of the invention to the research and development by cutting wound healing technology, promote cutting callus
Formed, solve the technical bottleneck that Rhododendron fortuneilindl． cuttage is difficult to take root, it is provided that a kind of rooting rate is high, seedling root is good, easy and simple to handle,
Practical, low cost, can the cuttage breeding method of large-scale cultivation Rhododendron fortuneilindl．, to meet the increasing market demand.
The technical scheme is that a kind of Rhododendron fortuneilindl． Callus formation and technology taken root of promoting, its step:
(1) seedbed is laid: seedbed bottom adds stone, cobblestone and rough sand the most successively, rough sand is installed ground hot line, then adds
Thicken the peat of 8-10 cm, perlite and Vermiculitum mixed-matrix, light pressure of raking, water permeable；Finally spray 500 times of carbendazim water
Substrate and surrounding enviroment are carried out disinfection by solution；(2) cutting collection: select age of tree 25-30, eugonic adult strain conduct
Mother plant for cutting, gathers the caulody of influences of plant crown band bud bud autumn, is cut into the cutting of long 12-13 cm；(3) cutting makes: remove base
Portion's blade, retains terminal bud and top vane 3-4 sheet, then cuts off leaf blade size 2/3；Then cutting base portion is carried out at two-sided wound healing
Reason (committed step)；(4) root induction: with 150 mg L-1Indolebutyric acid aqueous solution soaking cutting, natural air drying；(5) skewer
Insert and management: cutting process after cuttage immediately in the seedbed described in step (1), seeding row spacing 8 × 8 cm, the cuttage degree of depth is cutting
The 2/3 of length, sprays permeable；Arc plastic greenhouse is built on upper strata, seedbed, installs auto spraying facility, arrange movably outside canopy in canopy
Sunshade net；Period employing ground hot line of taking root carries out seedbed and heats, and automatic intermittent spraying system water supplement is humidified, and in canopy, temperature controls
15.0-20.0 DEG C, relative humidity 85.0-95.0%；Remove in time seedbed fallen leaves, and every 14 d spraying fungicide 1 time.
Substrate volume ratio described in step (1) is peat+perlite+Vermiculitum=3:1:1.
Cutting collection described in step (2) is to gather semi-lignified raw then late October to lignifying transition branch.
Two-sided wound healing described in step (3) processes and refers to cutting base portion be carried out lateral symmetry wound healing, wherein with grafting knife
Side wound healing mouth is long and shallow, long 1.5-2.0 cm, only up to cambium layer；Opposite side is short and deep, and long 0.5 cm, as deep as wooden marrow.
Root induction described in step (4) refers to that cutting base portion 2.0-2.5 cm soaks 16 in indolebutyric acid aqueous solution
h。
Heating and be set to 20 DEG C in seedbed described in step (5), automatic intermittent spraying is set to: daytime 2 min/2 h, evening
Upper 2 min/4 h；Sunshade is set to fine day sunshade 75%, cloudy day sunshade 50%, rainy day not sunshade.
The carbendazim aqueous solution of the thiophanate methyl that antibacterial is 1000 times described in step (5) and 500 times alternately makes
With.
It is an advantage of the current invention that: (1) early-stage Study finds that Rhododendron fortuneilindl． is that callus rooting types (do not give birth to by skin zone
Root).In order to improve cutting calli induction rate and callus area, the present invention develops a kind of new cutting and processes skill
Art: cutting base portion lateral symmetry wound healing processes, and side wound healing mouth is long and shallow, opposite side is short and deep.This wound healing method can be effective
Promote the generation of cutting callus, increase calli induction rate and callus area, thus life is greatly improved
Root rate, particularly dramatically increases quantity of taking root, improves cutting seedling quality and transplanting survival rate.(2) early-stage Study finds substrate
Composition and physicochemical character are to determine Rhododendron fortuneilindl． cutting Callus formation and the key factor taken root.In order to build optimal life
Root environment, substrate composition and proportioning are researched and developed, are found peat+perlite+Vermiculitum=3:1:1 volume ratio by the present invention again
Substrate is remarkably improved rooting rate, quantity of taking root and root length.(3) early-stage Study finds exogenous hormone scalable Rhododendron fortuneilindl． cutting
Physiological activity, promotion Callus formation and the startup of root restriction.In order to effectively regulate cutting physiological activity, the present invention couple
IBA concentration and soak time are optimized, and find 150 mg L-1Soaking with IBA cutting base portion 16 h be remarkably improved wound healing
Tissue formation, reduce rotting of cutting, and then improve rooting rate and quantity of taking root.(4) simultaneously, the inventive method is to ring of taking root
The climatic factor (illumination, temperature, humidity etc.) in border is optimized.(5) the inventive method is simple to operate, technology is easy, take root
Effective, material marketization is high, source is wide, be easily obtained, and is the effective ways of Rhododendron fortuneilindl． scale Fast-propagation.
Accompanying drawing explanation
Fig. 1 is that the two-sided wound healing of cutting of the present invention processes schematic diagram；
Fig. 2 is for using the inventive method cuttage Rhododendron fortuneilindl．, the 180th day situation of taking root.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further illustrated.
Embodiment 1
A kind of Rhododendron fortuneilindl． Callus formation and technology taken root of promoting, comprises the following steps:
1. seedbed is laid: seedbed is arranged in glasshouse, uses brick mix structure.Osculum is reserved every 80 cm bottom seedbed
1, bottom adds stone, cobblestone, rough sand the most successively, to increase draining and the aeration in seedbed；Pacify above coarse sand
Dress ground hot line, to increase ground temperature；Then peat+perlite+Vermiculitum=3:1:1 volume ratio the mixture of thick 8-10 cm, rake are laid
Flat gently press, make seedbed smooth, water permeable；Finally spray 500 times of carbendazim aqueous solutions substrate and surrounding enviroment are carried out disinfection.
2. cutting collection: select age of tree 25-30, eugonic adult strain to gather as mother plant for cutting, late October
Giving birth to semi-lignified then to lignifying transition branch, indoor are transported in water spray moisturizing in time back, choose branch sturdy upright, without pest and disease damage,
Without the sprig of petal (band bud bud), it is cut into the cutting of long 12-13 cm.
3. cutting makes: remove cutting radical leaves, retains terminal bud and top vane 3-4 sheet, then cuts off leaf blade size 2/
3, to reduce moisture evaporation；Then cutting base portion is carried out two-sided wound healing process: with grafting knife, cutting base portion is carried out both sides pair
Claim wound healing, wherein side wound healing mouth long and shallow (long 1.5-2.0 cm, only up to cambium layer), opposite side short and deep (grow 0.5 cm, deeply
Reach wooden marrow), with formation, the area of increase callus of callus induction, increase cutting to indolebutyric acid simultaneously
Absorb.
4. root induction: with 150 mg L-1IBA(indolebutyric acid) aqueous solution soaking cutting base portion (2.0-2.5 cm)
16 h, natural air drying.
5. cuttage and management: cuttage immediately after cutting process.First an aperture is drawn with small bamboo spike, to alleviate substrate during cuttage
Mechanical damage to cutting tissue；Seeding row spacing between cutting is 8 × 8 cm, and the cuttage degree of depth is the 2/3 of cutting length, sprays after cuttage
Permeable.For controlling environment epidemic disaster and the illumination in seedbed, arc plastic greenhouse is built on upper strata, seedbed, installs auto spraying and set in canopy
Execute, moveable sunshade net is set outside canopy.Period employing ground hot line of taking root carries out seedbed and heats, and ground temperature is set to 20 DEG C；Automatically
Intermittent spraying system water supplement be humidified: daytime 2 min/2 h, evening 2, min/4 h, gradually decreased water spray number of times after 1 month；In canopy
Temperature, intensity of illumination according to weather condition, ventilated by plastic greenhouse both sides respectively and sunshade net regulation (in canopy, temperature controls
15.0-20.0 DEG C, relative humidity 85.0-95.0%)；Pollute for reducing, remove seedbed in time and fall leaves and the cutting that rots, and every
14 the d carbendazim of 500 times or the thiophanate methyl aqueous solution alternating spray of 1000 times, with the generation of controlling disease.
When using the method cottage propagation Rhododendron fortuneilindl．, cuttage 6 months, statistics finds that its Callus formation rate reaches
100.00%, rooting rate be 73.67%, indefinite radical 21.45, the longest indefinite root length 6.33 cm, root 4.87 cm.
Embodiment 2
Compared with Example 1, differing only in, the cutting medium described in " step 1 " is the mixed of peat+perlite=3:1 volume ratio
Compound.
When using the method cottage propagation Rhododendron fortuneilindl．, cuttage 6 months, statistics finds that its Callus formation rate reaches
98.33%, rooting rate be 67.50%, indefinite radical 23.19, the longest indefinite root length 5.56 cm, root 4.26 cm.
Embodiment 3
Compare with the nursery effect of early-stage Study: early stage uses 6 kinds of substrate, two factorial experimentss of 4 IBA concentration to show, 24
Process in combination, " pure river sand substrate+200 mg L-1IBA aqueous solution soaking cutting base portion (1.5-2.0 cm) 16 h " combination
Nursery effect optimal, its Callus formation rate is 96.67%, rooting rate 60.00%, indefinite radical 20.31, the longest indefinite
Root length 4.00 cm, hat 3.18 cm.(see: " forest-science ", 2015,51 (9): 165-172)
As can be seen here, relative to prior art, the inventive method can improve the rooting rate of Rhododendron fortuneilindl． cutting propagation, the most aobvious
Work improves root system quality.
The principle of above-described embodiment only illustrative present invention and process thereof, not for limiting the present invention.Any ripe
Above-described embodiment all can be modified under the spirit and the scope of the present invention or change by the personage knowing this technology.Cause
This, have usually intellectual such as complete with institute under technological thought without departing from disclosed spirit in art
All equivalences become are modified or change, and must be contained by the claim of the present invention.
Claims (1)
1. promote Rhododendron fortuneilindl． Callus formation and the technology taken root, its step:
(1) seedbed is laid: seedbed bottom adds stone, cobblestone and rough sand the most successively, rough sand is installed ground hot line, so
The thick peat of 8-10 cm of rear interpolation, perlite and Vermiculitum mixed-matrix, light pressure of raking, water permeable；Finally spray bacterium more than 500 times
Substrate and surrounding enviroment are carried out disinfection by Lingshui Spring solution；
(2) cutting collection: select age of tree 25-30, eugonic adult strain to gather influences of plant crown as mother plant for cutting, autumn
With the caulody of bud bud, it is cut into the cutting of long 12-13 cm；
(3) cutting makes: remove radical leaves, retains terminal bud and top vane 3-4 sheet, then cuts off leaf blade size 2/3；The most right
Cutting base portion carries out two-sided wound healing process；
(4) root induction: with 150 mg L-1Indolebutyric acid aqueous solution soaking cutting, natural air drying；
(5) cuttage and management: cutting process after cuttage immediately in the seedbed described in step (1), seeding row spacing 8 × 8 cm, cuttage
The degree of depth is the 2/3 of cutting length, sprays permeable；Arc plastic greenhouse is built on upper strata, seedbed, installs auto spraying facility, canopy peripheral hardware in canopy
Put moveable sunshade net；Period employing ground hot line of taking root carries out seedbed and heats, and automatic intermittent spraying system water supplement is humidified, in canopy
Temperature controls at 15.0-20.0 DEG C, relative humidity 85.0-95.0%；Remove seedbed fallen leaves in time, and spray sterilization every 14 d
Agent 1 time.
A kind of Rhododendron fortuneilindl． Callus formation and technology taken root of promoting the most according to claim 1, its feature exists
In: the substrate volume ratio described in step (1) is peat+perlite+Vermiculitum=3:1:1.
A kind of Rhododendron fortuneilindl． Callus formation and technology taken root of promoting the most according to claim 1, its feature exists
In: the cutting collection described in step (2) is to gather semi-lignified raw then late October to lignifying transition branch.
A kind of Rhododendron fortuneilindl． Callus formation and technology taken root of promoting the most according to claim 1, its feature exists
In: the two-sided wound healing described in step (3) processes and refers to, with grafting knife, cutting base portion is carried out lateral symmetry wound healing, and wherein side is more
Wound length and shallow, long 1.5-2.0 cm, only up to cambium layer；Opposite side is short and deep, and long 0.5 cm, as deep as wooden marrow.
A kind of Rhododendron fortuneilindl． Callus formation and technology taken root of promoting the most according to claim 1, its feature exists
In: the root induction described in step (4) refers to that cutting base portion 2.0-2.5 cm soaks 16 h in indolebutyric acid aqueous solution.
A kind of Rhododendron fortuneilindl． Callus formation and technology taken root of promoting the most according to claim 1, its feature exists
In: heating and be set to 20 DEG C in the seedbed described in step (5), automatic intermittent spraying is set to: daytime 2 min/2 h, evening 2
min/4 h；Sunshade is set to fine day sunshade 75%, cloudy day sunshade 50%, rainy day not sunshade.
7. promoting Rhododendron fortuneilindl． Callus formation and the technology taken root according to a kind of described in claim 1, its feature exists
Carbendazim aqueous solution in: the thiophanate methyl that antibacterial is 1000 times described in step (5) and 500 times is used alternatingly.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107996370A (en) * | 2017-05-21 | 2018-05-08 | 贵州黔奥佳农业科技有限公司 | A kind of cultural method of the West cuckoo |
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