CN108093902A - A kind of method for promoting Kiwi berry cuttage root-taking using Siraitia grosvenorii leachate - Google Patents
A kind of method for promoting Kiwi berry cuttage root-taking using Siraitia grosvenorii leachate Download PDFInfo
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- CN108093902A CN108093902A CN201711249226.5A CN201711249226A CN108093902A CN 108093902 A CN108093902 A CN 108093902A CN 201711249226 A CN201711249226 A CN 201711249226A CN 108093902 A CN108093902 A CN 108093902A
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- cuttage
- siraitia grosvenorii
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
Abstract
The present invention relates to a kind of method for promoting Kiwi berry cuttage root-taking using Siraitia grosvenorii leachate, including the steps：Step S1, the preparation of Siraitia grosvenorii leachate；Step S2, the preparation of Kiwi berry cuttings；Rapid S3, cuttings is bundled, and lower end, which is placed in Siraitia grosvenorii leachate, impregnates 36 48h；Step S4 takes out the cuttings after impregnating, is rinsed with clear water, prepare cuttage after drying；Step S5, the preparation of slotting machine；Step S6, cuttage；Slotting machine is placed in ventilation by step S7, and control temperature makes the matrix of slotting machine keep moisture state at 20 25 DEG C；Step S8 at the beginning of 6 months, treats that Kiwi berry cuttage seeding adventitious root is grown, and is transplanted after root system more prosperity.Using this method, the cuttage seeding root system of acquisition is more flourishing, and fibrous root quantity and length, new-tip length and cuttage survival rate are substantially better than control and the processing of ABT root-inducing powders, and also provide a new thinking to the research of plant establishment mechanism.
Description
Technical field
The present invention relates to technical field of agricultural seedling-raising, and in particular to a kind of to promote Kiwi berry cuttage using Siraitia grosvenorii leachate
The method taken root.
Background technology
The cuttage of plant at present generally promotes it to take root using rush rooting parahormone, to obtain higher cuttage survival rate.
But the complexity of its individual cuttage root-taking of different plants even kindred plant different cultivars is occasionally there are bigger difference, especially
It is for the plant for being difficult to cuttage root-taking, comparatively ideal effect is obtained even if using root-growing agent is also difficult.This is just needed by a variety of
Means comprehensively utilize to improve its rooting rate.Have at present multiple using plant extract or extracting solution and other chemical compositions
Combination formula promotes the report of cuttage root-taking, but drug is various used in generally existing, configuration is complicated and cost is higher etc. asks
Topic.I chance in an experiment Siraitia grosvenorii leachate can improve Kiwi berry cuttage take root and survival rate, pass through
Further experiment designs, it is found that it has more significant facilitation of taking root to the Kiwifruit Cultivars of more difficult cuttage.
Kiwi berry also has relatively big difference because of the survival rate of species or its different cuttage of kind.Particularly Kiwi berry is given birth to
" China " common in production, " deliciousness " class Kiwi berry, cuttage survival rate is generally relatively low, quickly dipped soon using the root-growing agent of high concentration
The method that the root-growing agent of root or low concentration impregnates for a long time can promote it to take root to a certain extent, but effect is very unstable, no
It is larger to rooting parahormone concentration and the sensitivity differences of processing time with the Kiwi berry of species or kind, and the mistake of exogenous hormone
Amount impacts the follow-up nutrient growth of cuttings, dead seedling is be easy to cause after transplanting using the physiological equilibrium for easily upsetting cuttings
Or growing way is weaker, limits application and popularization of the cuttage technique in Kiwi berry production.
The content of the invention
In conclusion for overcome the deficiencies in the prior art, the technical problems to be solved by the invention are to provide a kind of utilization
The method that Siraitia grosvenorii leachate promotes Kiwi berry cuttage root-taking, the cuttage that can significantly improve difficult cuttage Kiwi berry are taken root
Rate, the root system after taking root is more flourishing, and transplanting survival rate is high, and growth is vigorous.This method is the excellent special macaque Peach rootstock of cultivation
It kind and lays a good foundation for the foundation of Kiwi berry cuttage and quick-propagation production technology.
The technical solution that the present invention solves above-mentioned technical problem is as follows：It is a kind of to promote Kiwi berry skewer using Siraitia grosvenorii leachate
The method taken root is inserted, including the steps：
Step S1, the preparation of Siraitia grosvenorii leachate：Healthy and full ripe Siraitia grosvenorii is selected, is placed on ventilating and cooling place
Saccharification 7-10 days is put into oven drying after surface is in slightly yellow；The Siraitia grosvenorii of drying is broken into pieces with one's fingers again, then together with pericarp one
It rises and is immersed in sterile hot water, impregnate, stirred therebetween for several times in the environment of the sterile hot water Temperature fall, finally cross and filter out
It is spare to obtain Siraitia grosvenorii leachate for slag；
Step S2, the preparation of Kiwi berry cuttings：At the end of February, select the annual degree of lignification being in hibernation higher
Kiwi berry branch, be cut into long 10-15cm and containing the cuttings of 2-3 full buds；In position of the cuttings upper end from terminal bud 1-1.5cm
Place is cut flat, and lower end is cut into inclined-plane at the position far from bottom bud 2-3cm；
Step S3, cuttings is bundled, and lower end, which is placed in Siraitia grosvenorii leachate, impregnates 36-48h；
Step S4 takes out the cuttings after impregnating, is rinsed with clear water, prepare cuttage after drying；
Step S5, the preparation of slotting machine：The preparation of slotting machine carries out in the greenhouse, and the treasure of one layer of 5-10cm is spread in bubble chamber bottom
Then Zhu Yan fills up sandy soil mixture that carbendazim sterilized as matrix, osculum and air hole is opened up on bubble chamber；
Step S6, cuttage：Cuttings lower end inclined-plane is made to be inserted into the 2/3 of training cuttings, phase upwards with slotting machine plane in 20 ° of angles
The spacing of adjacent cuttings is 5cm；To its top notch sealing wax after cuttings completion cuttage, then equably spray water and irrigate in matrix,
Obtain Kiwi berry cuttage seeding；
Slotting machine is placed in ventilation by step S7, and control temperature makes the matrix of slotting machine keep moisture state at 20-25 DEG C；
Step S8 at the beginning of 6 months, treats that the adventitious root of Kiwi berry cuttage seeding is grown, and is transplanted after root system more prosperity.
Research shows that some substances such as sucrose, vitamin C, amino-acid salt etc. are added in rooting process has one to taking root
Fixed facilitation.The nutriment that cuttings is consumed in rooting process is mainly carbohydrate and nitrogen compound, it
Not only provide cuttage rooting and growth nutriment, while be also cuttings base portion healing and new root energy substance.
C/N values can reflect its rootability well in cuttings, and the bigger rootability of C/N values is stronger within the specific limits, and cuttings is in life
Nutriment and other matter interactions, jointly promote the generation and growth of adventitious root during root.Siraitia grosvenorii leachate
In rich in glucoside, fructose, glucose, protein, lipid etc. and abundant minerals and vitamins etc., the wherein sweet tea of Momordia grosvenori aglycone
Degree is 300 times of sucrose or more.Obviously, metabolite special in Siraitia grosvenorii body plays facilitation to taking root, but whether
It is that its special Momordia grosvenori aglycone ingredient plays a key effect or the comprehensive function of other Multiple components need further to prove.
The beneficial effects of the invention are as follows：Using this method, Kiwifruit Cultivars " real beautiful " and " osmanthus of more difficult cuttage can be made
The cuttage survival rate of bird with red feathers " is respectively increased to 70% and more than 80%.The cuttage seeding root system of acquisition is more flourishing, fibrous root quantity and
Length, new-tip length and cuttage survival rate are substantially better than control and the processing of ABT root-inducing powders.Seedling is easy after transplanting, and growing way is good.Cause
The leachate that edible single Lo Han Guo fruit is only used for this method does not add other biochemical reagents as growth-promoting root reagent
Ingredient, it is simple and easy to use, securely and reliably.The hormones composition such as IAA, IBA, NAA containing facilitation of taking root is not added, no yet
Its normal physiological growth can be influenced due to agent concentration improper use of taking root causes the hormone imbalances of cuttings.This method not only has
Larger application practice meaning also provides a new thinking to the research of plant establishment mechanism.
Based on the above technical solutions, the present invention can also be improved as follows：
Further, the furnace drying method of Siraitia grosvenorii is in step S1：50 DEG C of low-temperature bakes improve temperature to 65 DEG C of bakings after 2-3 days
It is 2 days roasting, it then cools to 55 DEG C and continues baking 2-3 days, when the weight of fruit drops to the 25-30% of suitable fresh fruit weight,
Temperature fall obtains drying dried nuts.
Further, the temperature of sterile hot water described in step S1 is 80-90 DEG C, and weight is 30 times of Siraitia grosvenorii；It impregnates
Time be 12-16h.
Further, the inclined-plane of cuttings lower end and terminal bud are in the same side of cuttings in step S2.
Further, sandy soil mixture includes rough sand and loam in step S5, and the weight ratio of the rough sand and loam is 2：1.
Further, in step S5 there are two osculums, a diameter of 1cm and the bottom in bubble chamber；Air hole has 4-5
It is a, a diameter of 1cm and in bubble chamber four side walls and at the position of bottom 5-7cm.
Specific embodiment
The principle of the present invention and feature are described below in conjunction with specific example, example is served only for explaining this hair
It is bright, it is not intended to limit the scope of the present invention.
Embodiment 1
(1) preparation of Siraitia grosvenorii leachate：A healthy and full ripe Siraitia grosvenorii is selected, places ventilating and cooling place sugar
Change 7-10 days, slightly dry in being put into oven drying after yellow, break into pieces with one's fingers after surface, be immersed in together with pericarp 700mL, 90 DEG C
About 16h in sterile hot water.It is spare to retain Siraitia grosvenorii leachate for filtering and removing slag.
(2) preparation of Kiwi berry branch：Mid-February, select the annual degree of lignification being in hibernation higher
" real beautiful " and " osmanthus bird with red feathers " Kiwi berry branch, are cut into cuttings of the long 10-15cm or so containing 2-3 full buds, are held on it from terminal bud
1cm or so places are cut flat, and lower end is cut into inclined-plane at away from bottom bud 2-3cm (in the identical faces of cuttings terminal bud).
(3) cuttings is bundled, lower end, which is placed in Siraitia grosvenorii leachate, impregnates 30h.
(4) cuttings after impregnating is taken out, and is rinsed with clear water, prepares cuttage after slightly drying.
(5) preparation of slotting machine：The preparation of slotting machine carries out in the greenhouse, using being about 55cm, width about 40cm, high about 20cm
Bubble chamber, bottom spread the perlite of one layer of 5-10cm or so, then fill up sandy soil mixture (rough sand and earth that carbendazim sterilized
Local soil type is into the weight ratio of the two is 2：1).The apopore of 2 diameter 1cm or so is opened in the bottom of bubble chamber, the four of bubble chamber
A side wall opens the ventilation mouth of 4-5 diameter 1cm or so apart from the position of bottom 5-7cm respectively.
(6) cuttage.Make cuttings lower end inclined-plane upward, with slotting machine plane in 20 ° of angles, the 2/3 of insertion training cuttings, between cuttings
Away from for 5cm.After cuttings has been inserted, by cuttings top notch sealing wax.
(7) equably sprayed water, irrigated in matrix with kettle.Slotting machine is placed in ventilation, control temperature is on a 20-25 DEG C of left side
It is right.Frequent water drenching makes slotting machine keep moistening.
(8) count and transplant：At the beginning of 6 months, rooting rate, adventitious root quantity, root length and the new-tip length of cuttage seeding are investigated.
Potted plant growth in peat soil matrix is transplanted to after the completion of statistics.
Embodiment 2
The reagent of taking root used is ABT root-inducing powder：1g ABT root-inducing powders are dissolved in 50ml alcohol, are stirred well to completely molten
Solution, concentration 2g/L is diluted to sterile water.By the cuttings handled well quickly dipped 5 seconds in the solution after dilution, rapid cuttage in
In culture substrate.Remaining process is the same as embodiment 1.
Embodiment 3
The ABT root-inducing powders concentration used is 0.2g/L, cuttings is impregnated to cuttage after 6h in the solution, remaining is the same as embodiment 3.
Embodiment 4
Without using any solution or agent treatment, directly it is inserted into after cuttings processing on slotting machine.Remaining operation is same
Embodiment 1.
Table 1 shows " mean elements ", " average root of the 4 kinds of processing modes of embodiment 1-4 to two kinds of Kiwi berry cuttage root-takings
The influence of length ", " average new-tip length " and " root survival ".
Influence of the 1 different disposal method of table to " real beautiful " and " osmanthus bird with red feathers " Kiwi berry cuttage survival rate
As can be seen from Table 1, after being impregnated using Siraitia grosvenorii leachate to cuttings, the mean elements of two Kiwifruit Cultivars,
Average root long, average new-tip length and root survival are all higher than without processing control and using the processing control of ABT root-inducing powders.Wherein
Most notably on mean elements and cuttage survival rate, it is significantly higher than other processing.And use different ABT root-inducing powders processing sides
Formula the result shows that, the quickly dipped rooting efficiency of high concentration will be significantly better than low concentration impregnate effect.Low concentration ABT root-inducing powders soak
The effect of bubble is not significantly different with being compareed without processing, illustrates ABT root-inducing powder difference concentrations and Different treatments to Mi
Monkey peach cuttage root-taking is affected.The complexity of taking root of " real beautiful " and " osmanthus bird with red feathers " Kiwi berry has relatively big difference, but by sieve
Comparatively ideal root survival has been obtained after Chinese fruit leachate immersion treatment, has illustrated the processing of Siraitia grosvenorii leachate to Kiwi berry skewer
It inserts and takes root with obvious facilitation.ABT root-inducing powders have been widely used and have confirmed have apparent promote to plant establishment
Into effect, although its rooting efficiency is less than the effect of Siraitia grosvenorii leachate immersion treatment in present treatment, can't therefore obtain
The rooting efficiency for going out Siraitia grosvenorii leachate is centainly better than or can substitute the conclusion of ABT root-inducing powders, because variety classes or kind
Plant it is larger to the sensitivity differences of steroids reagent, what this experiment used be not necessarily ABT root-inducing powders it is optimal use it is dense
Degree and processing time, and the application effect of Siraitia grosvenorii leachate is not also verified on more plants, it is also necessary to more
The support of extensive experimental data.But do not influence " Siraitia grosvenorii leachate can remarkably promote Kiwi berry cuttage and take root " conclusion
Draw.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all the present invention spirit and
Within principle, any modifications, equivalent replacements and improvements are made should all be included in the protection scope of the present invention.
Claims (6)
- A kind of 1. method for promoting Kiwi berry cuttage root-taking using Siraitia grosvenorii leachate, which is characterized in that including the steps：Step S1, the preparation of Siraitia grosvenorii leachate：Healthy and full ripe Siraitia grosvenorii is selected, is placed on ventilating and cooling place saccharification 7-10 days, oven drying is put into after surface is in slightly yellow；The Siraitia grosvenorii of drying is broken into pieces with one's fingers again, is then soaked together with pericarp Bubble is impregnated in the environment of the sterile hot water Temperature fall, stirred therebetween for several times, last filtering and removing slag in sterile hot water, It is spare to obtain Siraitia grosvenorii leachate；Step S2, the preparation of Kiwi berry cuttings：At the end of February, the Mi for selecting the annual degree of lignification being in hibernation higher Monkey peach branch is cut into long 10-15cm and containing the cuttings of 2-3 full buds；It is cut at position of the cuttings upper end from terminal bud 1-1.5cm Flat, lower end is cut into inclined-plane at the position far from bottom bud 2-3cm；Step S3, cuttings is bundled, and lower end, which is placed in Siraitia grosvenorii leachate, impregnates 36-48h；Step S4 takes out the cuttings after impregnating, is rinsed with clear water, prepare cuttage after drying；Step S5, the preparation of slotting machine：The preparation of slotting machine carries out in the greenhouse, and the pearl of one layer of 5-10cm is spread in bubble chamber bottom Then rock fills up sandy soil mixture that carbendazim sterilized as matrix, osculum and air hole is opened up on bubble chamber；Step S6, cuttage：Make cuttings lower end inclined-plane upwards with slotting machine plane in 20 ° of angles, the 2/3 of insertion training cuttings, it is adjacent to insert The spacing of fringe is 5cm；It to its top notch sealing wax after cuttings completion cuttage, then equably sprays water and irrigates in matrix, obtain Kiwi berry cuttage seeding；Slotting machine is placed in ventilation by step S7, and control temperature makes the matrix of slotting machine keep moisture state at 20-25 DEG C；Step S8 at the beginning of 6 months, treats that the adventitious root of Kiwi berry cuttage seeding is grown, and is transplanted after root system more prosperity.
- 2. the method according to claim 1 for promoting Kiwi berry cuttage root-taking using Siraitia grosvenorii leachate, which is characterized in that The furnace drying method of Siraitia grosvenorii is in step S1：50 DEG C of low-temperature bakes improve temperature and are toasted 2 days to 65 DEG C after 2-3 days, then cool down Continue baking 2-3 days to 55 DEG C, when the weight of fruit drops to the 25-30% of fresh fruit weight, Temperature fall is dried Fruit.
- 3. the method according to claim 1 for promoting Kiwi berry cuttage root-taking using Siraitia grosvenorii leachate, which is characterized in that The temperature of sterile hot water described in step S1 is 80-90 DEG C, and weight is 30 times of Siraitia grosvenorii；The time of immersion is 12-16h.
- 4. the method according to claim 1 for promoting Kiwi berry cuttage root-taking using Siraitia grosvenorii leachate, which is characterized in that The inclined-plane of cuttings lower end is in the same side of cuttings with terminal bud in step S2.
- 5. the method according to claim 1 for promoting Kiwi berry cuttage root-taking using Siraitia grosvenorii leachate, which is characterized in that Sandy soil mixture includes rough sand and loam in step S5, and the weight ratio of the rough sand and loam is 2：1.
- 6. the method according to claim 1 for promoting Kiwi berry cuttage root-taking using Siraitia grosvenorii leachate, which is characterized in that In step S5 there are two osculums, a diameter of 1cm and the bottom in bubble chamber；Air hole has 4-5, a diameter of 1cm And in bubble chamber four side walls and at the position of bottom 5-7cm.
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| CN201711249226.5A CN108093902B (en) | 2017-12-01 | 2017-12-01 | Method for promoting cuttage rooting of kiwi fruits by using momordica grosvenori lixivium |
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Citations (5)
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| CN1076333A (en) * | 1993-03-10 | 1993-09-22 | 江西省庐山植物园 | A kind of plant rooting promoter and its production and use |
| CN105265149A (en) * | 2015-10-27 | 2016-01-27 | 界首市聚丰家庭农场 | High-yield planting method of kiwi fruit in north of Anhui province |
| CN105612996A (en) * | 2015-12-30 | 2016-06-01 | 浙江农林大学 | Chinese torreya cutting propagation method |
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-
2017
- 2017-12-01 CN CN201711249226.5A patent/CN108093902B/en not_active Expired - Fee Related
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| CN1076333A (en) * | 1993-03-10 | 1993-09-22 | 江西省庐山植物园 | A kind of plant rooting promoter and its production and use |
| CN105265149A (en) * | 2015-10-27 | 2016-01-27 | 界首市聚丰家庭农场 | High-yield planting method of kiwi fruit in north of Anhui province |
| CN105612996A (en) * | 2015-12-30 | 2016-06-01 | 浙江农林大学 | Chinese torreya cutting propagation method |
| CN106034929A (en) * | 2016-06-17 | 2016-10-26 | 枞阳县白云生态园林有限责任公司 | High-quality Actinidia chinensis planting method |
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