CN101983242B - 含有尿素类氮源的发酵培养基及其用于生产重组蛋白的用途 - Google Patents

含有尿素类氮源的发酵培养基及其用于生产重组蛋白的用途 Download PDF

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CN101983242B
CN101983242B CN200980112059.7A CN200980112059A CN101983242B CN 101983242 B CN101983242 B CN 101983242B CN 200980112059 A CN200980112059 A CN 200980112059A CN 101983242 B CN101983242 B CN 101983242B
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urea
fermentation
fermentation medium
insulin
medium
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CN101983242A (zh
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桑贾伊·蒂瓦里
穆克什·巴布阿帕·帕塔勒
沙鲁巴·加格
马扬克·库马·加格
苏勒克哈·约希
奇特纳利·拉梅高达·那文·库马
比马尔·库马
安努杰·格尔
哈里什·耶尔
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Biocon Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CN200980112059.7A 2008-02-06 2009-02-05 含有尿素类氮源的发酵培养基及其用于生产重组蛋白的用途 Active CN101983242B (zh)

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Application Number Priority Date Filing Date Title
CN201310522914.XA CN103642843B (zh) 2008-02-06 2009-02-05 含有尿素类氮源的发酵培养基及其用于生产次级代谢产物、酶和重组蛋白的用途

Applications Claiming Priority (5)

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IN310CH2008 2008-02-06
IN00310/CHE/2008 2008-02-06
IN00086/CHE/2009 2009-01-12
IN86CH2009 2009-01-12
PCT/IN2009/000078 WO2009113099A2 (en) 2008-02-06 2009-02-05 Fermentation medias and processes thereof

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CN101983242B true CN101983242B (zh) 2015-12-16

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CN201310522914.XA Active CN103642843B (zh) 2008-02-06 2009-02-05 含有尿素类氮源的发酵培养基及其用于生产次级代谢产物、酶和重组蛋白的用途

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US (2) US8927256B2 (enExample)
EP (2) EP2565272A3 (enExample)
JP (1) JP5587795B2 (enExample)
KR (2) KR101283734B1 (enExample)
CN (2) CN101983242B (enExample)
BR (1) BRPI0905853B1 (enExample)
IL (2) IL207402A (enExample)
MX (2) MX2010008654A (enExample)
MY (1) MY161108A (enExample)
RU (3) RU2595380C2 (enExample)
SG (1) SG188126A1 (enExample)
WO (1) WO2009113099A2 (enExample)

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HUE035803T2 (en) 2012-12-21 2018-05-28 Sanofi Sa Dual GLP1 / GIP or trigonal GLP1 / GIP / glucagon agonists
CN103911298B (zh) * 2012-12-31 2016-08-10 中粮营养健康研究院有限公司 培养基组合物和培养基及对菌株进行活菌计数的方法
WO2015086728A1 (en) 2013-12-13 2015-06-18 Sanofi Exendin-4 peptide analogues as dual glp-1/gip receptor agonists
EP3080154B1 (en) 2013-12-13 2018-02-07 Sanofi Dual glp-1/gip receptor agonists
TW201609797A (zh) 2013-12-13 2016-03-16 賽諾菲公司 雙重ｇｌｐ－１／升糖素受體促效劑
WO2015086730A1 (en) 2013-12-13 2015-06-18 Sanofi Non-acylated exendin-4 peptide analogues
TW201625668A (zh) 2014-04-07 2016-07-16 賽諾菲公司 作為胜肽性雙重ｇｌｐ－１／昇糖素受體激動劑之艾塞那肽－４衍生物
TW201625669A (zh) 2014-04-07 2016-07-16 賽諾菲公司 衍生自艾塞那肽－４（Ｅｘｅｎｄｉｎ－４）之肽類雙重ＧＬＰ－１／升糖素受體促效劑
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US9932381B2 (en) 2014-06-18 2018-04-03 Sanofi Exendin-4 derivatives as selective glucagon receptor agonists
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TW201706291A (zh) 2015-07-10 2017-02-16 賽諾菲公司 作為選擇性肽雙重ｇｌｐ－１／升糖素受體促效劑之新毒蜥外泌肽（ｅｘｅｎｄｉｎ－４）衍生物
WO2017070200A1 (en) * 2015-10-20 2017-04-27 Buckman Laboratories International, Inc. Method to enhance yeast growth for fermentative bioproduct production, and nutrient composition for same
WO2017125885A1 (en) * 2016-01-23 2017-07-27 Biocon Limited Bio-analytical method for insulin analogues
CN107022591B (zh) * 2017-05-11 2018-06-12 宜昌东阳光长江药业股份有限公司 一种提高胰岛素及其类似物前体表达的毕赤酵母发酵方法
WO2018216978A1 (en) * 2017-05-22 2018-11-29 Cj Healthcare Corporation Cell culture method at low temperature applicable to mass production of protein
CN107988292B (zh) * 2018-01-18 2023-12-26 江苏江山聚源生物技术有限公司 提高重组人源胶原蛋白稳定性的发酵工艺
CN111411049B (zh) * 2020-04-15 2022-11-22 北京惠之衡生物科技有限公司 一种提高胰岛素前体表达的毕赤酵母的发酵培养基及发酵方法
CN111996136A (zh) * 2020-07-24 2020-11-27 北大方正集团有限公司 一种荧光假单孢杆菌发酵培养基、培养方法与应用
CN114507609B (zh) * 2020-12-22 2023-04-18 益海嘉里(连云港)生物科技有限公司 一种提高磷脂酶c蛋白产量的方法
CN117467716A (zh) * 2022-07-22 2024-01-30 上海医药工业研究院有限公司 一种提高洛伐他汀发酵单位的发酵培养基及其应用

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MX2010008654A (es) 2010-10-06
US20140141467A1 (en) 2014-05-22
KR20120066068A (ko) 2012-06-21
IL207402A0 (en) 2010-12-30
US8927256B2 (en) 2015-01-06
RU2010137009A (ru) 2012-03-20
EP2565272A2 (en) 2013-03-06
MX349413B (es) 2017-07-19
CN101983242A (zh) 2011-03-02
IL207402A (en) 2013-07-31
MY161108A (en) 2017-04-14
IL221379A (en) 2013-08-29
IL221379A0 (en) 2012-10-31
EP2247737B1 (en) 2018-06-20
EP2565272A3 (en) 2013-07-03
WO2009113099A3 (en) 2009-11-05
RU2556120C2 (ru) 2015-07-10
US20100317056A1 (en) 2010-12-16
RU2012145969A (ru) 2014-05-10
SG188126A1 (en) 2013-03-28
JP2011510677A (ja) 2011-04-07
CN103642843B (zh) 2017-03-01
EP2247737A4 (en) 2012-03-14
EP2247737A2 (en) 2010-11-10
BRPI0905853A8 (pt) 2019-01-29
RU2011153811A (ru) 2013-07-10
US9255285B2 (en) 2016-02-09
RU2595380C2 (ru) 2016-08-27
JP5587795B2 (ja) 2014-09-10
WO2009113099A2 (en) 2009-09-17
RU2491345C2 (ru) 2013-08-27
CN103642843A (zh) 2014-03-19
BRPI0905853B1 (pt) 2019-03-26
KR20100118592A (ko) 2010-11-05
KR101283734B1 (ko) 2013-07-08
BRPI0905853A2 (pt) 2015-06-30
KR101492011B1 (ko) 2015-02-10

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