CN101884294A - Method for reducing juvenile period of hybrid tangerine - Google Patents
Method for reducing juvenile period of hybrid tangerine Download PDFInfo
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- CN101884294A CN101884294A CN 201010210503 CN201010210503A CN101884294A CN 101884294 A CN101884294 A CN 101884294A CN 201010210503 CN201010210503 CN 201010210503 CN 201010210503 A CN201010210503 A CN 201010210503A CN 101884294 A CN101884294 A CN 101884294A
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Abstract
The invention relates to a method for reducing juvenile period of hybrid tangerine, which comprises the following steps: culturing hybrid seedlings till the hybrid seedlings grow two true leaves and two stages of roots; performing high grafting of the hybrid seedlings onto tangerine branches which have closer relationships with grafts, is free from diseases and insects and 100 centimeters above the ground and have diameters more than 3 centimeters; and finally, performing final-period management such as tree shape control and girdling for promoting promoting flower-formation. When the method is used, the juvenile period of hybrid tangerine trees is reduced to about 2 years, and the juvenile period of some hybrid tangerine trees is reduced to 1 year. Compared with the conventional cultivation method of hybrid tangerine breeding seedlings, the method has the advantages of greatly reducing breeding period and accelerating the breeding of tangerine hybridization breeding.
Description
Technical field
The present invention relates to the crossbreeding of oranges and tangerines, relate in particular to a kind of method that shortens juvenile period of hybrid tangerine.
Background technology
The oranges and tangerines crossbreeding technology can be fundamentally by the mode of genetic recombination, makes the comprehensive parents' of filial generation oranges and tangerines new varieties advantage, have parents' excellent proterties concurrently, but the breeding cycle of conventional hybridization oranges and tangerines is long, and it is very long that its main cause is exactly the virgin phase of oranges and tangerines.The virgin phase of oranges and tangerines is meant that oranges and tangerines are from year time number that planting seed experiences to blooming.Generally, behind the oranges and tangerines planting seed, arrive the 7th year or just began to bloom in the 8th year, promptly the virgin phase of oranges and tangerines is taken 7～8 years, adds that follow-up horticultural characteristics is observed with genetic stability to identify, breeds a hybrid tangerine kind and on average takes consuming time more than 18 years.This characteristic has greatly hindered the raising of oranges and tangerines breeding work efficient, also causes oranges and tangerines to become one of the longest fruit tree of crossbreeding cycle.Therefore, shorten the research of virgin phase of oranges and tangerines and become the key point that improves oranges and tangerines breeding work efficient.
The existing known U.S. that carries out the oranges and tangerines crossbreeding the earliest, though the crossbreeding history in 116 years is arranged, the hybrid tangerine kind that it brings out is very few.Japan has specialized in the culture technique of hybrid tangerine in the period of 1960～1970, make the virgin phase of oranges and tangerines shorten, but the shortlyest also takes 4 years (seeing Table 1).After this, do not make further progress in the field for the research of shortening the virgin phase of oranges and tangerines.
The virgin phase of the Japanese hybrid tangerine of table 1
| The hybrid tangerine kind of breeding | Hybridization time | Solid for the first time | Breed the time | Virgin phase/year |
| ス イ-ト ス プ リ Application ゲ (sweet spring) | ??1947 | ??1964 | ??1981 | ??16 |
| See clearly | ??1949 | ??1961 | ??1979 | ??11 |
| Tianjin 20 of mouth | ??1966 | ??1982 | ??1986 | ??15 |
| Clear peak | ??1971 | ??1977 | ??1981 | ??5 |
| The perfume (or spice) in Tianjin | ??1972 | ??1978 | ??1990 | ??5 |
| Do not know fire | ??1972 | ??1978 | ??5 | |
| Sun is fragrant | ??1972 | ??1978 | ??1995 | ??5 |
| Have bright | ??1973 | ??1978 | ??1994 | ??4 |
| It is fragrant | ??1974 | ??1984 | ??1996 | ??9 |
| Zhu sees | ??1975 | ??1985 | ??1996 | ??9 |
| The hybrid tangerine kind of breeding | Hybridization time | Solid for the first time | Breed the time | Virgin phase/year |
| See the spring | ??1979 | ??1986 | ??1996 | ??6 |
| ??S1128 | ??1981 | ??1986 | ??4 | |
| It grass | ??1982 | ??1987 | ??1993 | ??4 |
| Beautiful red | ??1984 | ??1989 | ??2005 | ??4 |
| The brightness in Tianjin | ??1984 | ??1989 | ??2008 | ??4 |
| Southwest | ??1988 | ??1995 | ??2009 | ??6 |
| Like beautiful woman No. 28 | ??1990 | ??1995 | ??2005 | ??4 |
In the prior art about cultivating the method for hybrid tangerine kind, it all will experience the seedling stage, and (seedling is meant seed germination and the sapling that forms, be different from by grafting the seedling that comes thus), the stages such as grafting stage and final-period management, these stages all will have influence on the virgin phase length of hybrid tangerine, and the crossbreeding cycle of existing method is all veryer long.
Summary of the invention
The object of the present invention is to provide a kind of method that shortens juvenile period of hybrid tangerine, this method is accelerated breeding process in order to shorten the virgin phase of hybrid tangerine.
The object of the present invention is achieved like this: a kind of method that shortens juvenile period of hybrid tangerine is characterized in that:
The cultivation of its hybrid seedling is to cultivate to growing two true leaves and secondary root system;
Its high position grafting is performed such: earlier the root system below the hybrid seedling hypocotyl is pruned, remaining part is as a scion; Select with the scion affiliation closely, and do not have damage by disease and insect, more than the liftoff 100cm, the above oranges and tangerines branch of diameter 3cm is as interstock, in sowing then mid-February to grafting mid-March, its every interstock on grafting one strain scion; Place the sunshade of 45%～60% obscurity off the net afterwards, treat to separate when the new long terminal bud of scion or arbitrary lateral bud length reach 0.5～1.5cm film;
Its final-period management is performed such: after treating that scion grows young sprout, one tender shoots of selecting and remain the most sturdy is cultivated as permanent branch, and the traction thing is set guarantees that it is tree-like upright, in time remove lateral bud and side shoot on the permanent branch in the incubation, treat that permanent branch length is that 200cm stays branch after above; Grafting in May, second early and middle ten days carries out band girdle to permanent branch and loses money instead of making money skin, late August once more band girdle lose money instead of making money skin.
The cultivation of above-mentioned hybrid seedling, preferably adopt such method to carry out: with the hybrid oranges and tangerines seed of maturation, peel off outer, interior two-layer kind of skin, no soil is cultured to seedling and grows two true leaves and secondary root system.Adopt such cultured method, can obtain healthy and strong seedling, be beneficial to its growth and cultivate, can improve cultivation speed.
During above-mentioned high position grafting, it is the contact-making surface of 1～2cm that hybrid seedling is whittled into the face length of splaying from hypocotyl, and as a scion, such scion and interstock are in conjunction with best results with the part of whole pearl seedling remainder.
When above-mentioned band girdle was lost money instead of making money skin, a stripping mouthful width was 2～3cm, and this stripping mouthful width is suitable, helps short flower.
In this method, the rich water quality management method is identical with management method with general citriculture.
The inventive method is operation so specifically:
1, the cultivation of hybrid seedling: the fully ripe hybrid oranges and tangerines seed of gathering, peel off outer, interior two-layer kind of skin, no earth culture is educated seedling in incubator.Be specially: at the cuboid plastic culture case of 15 * 15 * 30cm, case heelpiece thickness is the aseptic filter paper of 10cm, with running water wetting after, keep having at the bottom of the case degree of depth to be no more than the water of 1cm, cultivate 100～150 in seed peelling off kind of skin in every case, indoor 25～30 ℃ of constant temperature, daylight 10 hours, vernalization under the evening dark condition, the case mouth is tightened with polyethylene film and is preserved moisture, and separates the film ventilation once in per 2～3 days.After treating that hybrid seedling grows two true leaves and secondary root system, be directly used in high position grafting.
2, high position grafting: earlier the root system below the hybrid seedling hypocotyl is pruned, being whittled into the face length of splaying at the hypocotyl place is the contact-making surface of 1～2cm, thisly removes the seedling that root system has contact-making surface and is scion; Select with the scion affiliation closely, and do not have damage by disease and insect, more than the liftoff 100cm, the above oranges and tangerines branch of diameter 3cm is as interstock, in sowing then mid-February to grafting mid-March, its every interstock on grafting one strain scion; Place off the net the preventing of the sunshade of 45% obscurity day to burn afterwards, treat to separate when 0.5～1.5cm is grown in scion terminal bud or either side blastogenesis film.
3, final-period management: after treating that scion grows young sprout, a spray of selecting and remain the most sturdy is cultivated as permanent branch, and at the other traction thing that is provided with of the seedling of every strain grafting, as bamboo pole, waddy or steel bar etc., guarantee that it is tree-like upright, in time remove lateral bud and side shoot on permanent in the incubation, only stay the upwards growth of a terminal bud, treat that the long 200cm of being of permanent branch stays branch after above; Grafting in May, second early and middle ten days carries out band girdle to permanent branch and loses money instead of making money skin, and a stripping mouthful width is 2～3cm, late August once more band girdle lose money instead of making money skin, a stripping mouthful width is 2～3cm, the water and fertilizer management mode is identical with the general cultivation management method of oranges and tangerines.
The present invention has beneficial effect as described below:
The inventive method has shortened to the virgin phase of hybrid tangerine about 2 years, and the breeding method of the existing hybrid tangerine breeding station of comparing has shortened breeding cycle greatly, has accelerated the process of oranges and tangerines crossbreeding.Adopt the inventive method, in the crossbreeding field, improved breeding efficiency greatly, make the crossbreeding R﹠D costs descend significantly, average every strain hybrid seedling is yielded positive results from being seeded into, and the cost that carries out the evaluation of horticultural characteristics has for the first time reduced more than 80%.
Embodiment
Below by embodiment the present invention is specifically described; be necessary to be pointed out that at this; following examples only are used for the present invention is further detailed; can not be interpreted as limiting the scope of the invention, the person skilled in the art in this field can make some nonessential improvement and adjustment to the present invention according to the foregoing invention content.
Embodiment 1
The present embodiment employing does not know that fiery tangerine orange is the examination material, sows cultivation in January, 2006, wherein, does not know that fiery tangerine orange is to breed by seeing that clearly tangerine orange and middle wild No. 3 ponkans are hybridized, and introduces Chinese in 1992 years from Japan.This kind is the kind of the polyembryony type of a strictness, i.e. the genetic material height unanimity of the seedling of all cultivating seeds one-tenth.This kind is in the breeding process in Japanese original producton location, and its virgin phase is 5 years.
This embodiment step is:
1), the cultivation of hybrid seedling: the fully ripe fiery tangerine orange seed of not knowing of gathering, peel off outer, the interior two-layer kind of skin of seed, no earth culture is educated seedling in incubator.Be specially: at the cuboid plastic culture case of 15 * 15 * 30cm, case heelpiece thickness is the aseptic filter paper of 10cm, with running water wetting after, at the bottom of the maintenance case degree of depth being arranged is the water of 1cm, cultivate 150 in seed peelling off kind of skin in every case, indoor 25 ℃ of constant temperature, vernalization under 10 hours conditions of illumination every day, the case mouth is tightened with polyethylene film and is preserved moisture, and separates the film ventilation once in per 2 days.After treating that hybrid seedling grows two true leaves and secondary root system, choose 100 pearls, be directly used in high position grafting.
2), high position grafting: earlier the root system below the hybrid seedling hypocotyl is pruned, remaining part is as a scion, and the face length of splaying of formation is the contact-making surface of 1.2cm; Select with the scion affiliation closely, and do not have damage by disease and insect, liftoff 110cm, 6 years living orange branches of anti-summer of trifoliate orange anvils honey of diameter 3cm are as interstock, in sowing early March grafting then, its every interstock on grafting 1 strain scion; Place the sunshade net of 45% obscurity to prevent from day to burn down afterwards, separate film when treating the long 0.5cm of new long terminal bud of scion or lateral bud.
3), final-period management: after treating that scion grows young sprout, a tender shoots of selecting and remain the most sturdy is cultivated as permanent branch, and in the other rope bondage that the high bamboo pole of 300cm is set with softness of the seedling of every strain grafting, guarantee that it is tree-like upright, in time remove lateral bud and side shoot on the permanent branch in the incubation, only stay the upwards growth of a terminal bud, treat the long branch that stays during for 200cm of permanent branch; Grafting the first tenday period of a month in May, second permanent branch is carried out band girdle and lose money instead of making money skin, a stripping mouthful width is 2.3cm, late August once more band girdle lose money instead of making money skin, a stripping mouthful width is 2.5cm, the water and fertilizer management mode is identical with the general cultivation management method of oranges and tangerines.
Comparative Examples 1
Adopt the fiery tangerine orange seed of not knowing among the embodiment 1, plant 50 strains in the field, its seeding row spacing carries out the routine plantation and cultivates by 2 * 3m.
Comparative Examples 2
Adopt the fiery tangerine orange seed of not knowing among the embodiment 1, breeding method is cultivated seedling 100 strains that obtain not knowing fiery tangerine orange routinely, carries out conventional high position grafting and final-period management in early March, and wherein, the high position grafting interstock is selected 6 years living orange branches of anti-summer of trifoliate orange anvil honey for use.
Result of the test sees the following form 2:
Table 2 does not know that the virgin phase of fiery tangerine orange and corresponding strain number compare (unit: strain)
| Handle | Seedling | Survive seedling | 2 years virgin phases | 3 years virgin phases | 4 years virgin phases |
| Comparative Examples 1 | ??50 | ??41 | ??0 | ??0 | ??0 |
| Comparative Examples 2 | ??100 | ??48 | ??0 | ??0 | ??2 |
| Embodiment 1 | ??100 | ??98 | ??98 |
As can be seen, the breeding method in Comparative Examples 1 and the Comparative Examples 2 does not know that the virgin phase of fiery tangerine orange is very long, at least all takes 4 years, and this method breeding cycle is long, inefficiency.
Embodiment 2
Present embodiment adopts the spring to see that the tangerine orange for the examination material, sows cultivation in January, 2006, and the spring sees that the tangerine orange is by seeing that clearly tangerine orange and ponkan F-2432 number hybridization breeds, and 2002 years Chinese from Japan's introducing.This kind is the kind of the polyembryony type of a strictness, i.e. the genetic material height unanimity of the seedling of all cultivating seeds one-tenth.This kind is in the breeding process in Japanese original producton location, and its virgin phase is 5 years.
1, the cultivation of hybrid seedling: the fully ripe spring of gathering is seen tangerine orange seed, peels off outer, the interior two-layer kind of skin of seed, and no earth culture is educated seedling in incubator.Be specially: at the cuboid plastic culture case of a plurality of 15 * 15 * 30cm, case heelpiece thickness is the aseptic filter paper of 10cm, with running water wetting after, at the bottom of the maintenance case degree of depth being arranged is the water of 0.8cm, cultivate 150 in seed peelling off kind of skin in every case, indoor 30 ℃ of constant temperature, vernalization under 10 hours conditions of illumination every day, the case mouth is tightened with polyethylene film and is preserved moisture, and separates the film ventilation once in per 3 days.After treating that hybrid seedling grows two true leaves and secondary root system, chose for 500 pearl spring altogether and see tangerine orange seedling, be directly used in high position grafting.
2, high position grafting: earlier the root system below the hybrid seedling hypocotyl is pruned, remaining part is as a scion, and the face length of splaying of formation is the contact-making surface of 2cm; Select with the scion affiliation closely, and do not have damage by disease and insect, liftoff 150cm, 6 years living orange branches of anti-summer of trifoliate orange anvils honey of diameter 4cm are as interstock, in sowing late Febuary grafting then, its every interstock on grafting one strain scion; Place the sunshade net of 45% obscurity to prevent from day to burn down afterwards, separate film when treating the long 1.3cm of the new long arbitrary lateral bud of scion.
3, final-period management: after treating that scion grows young sprout, a spray of selecting and remain the most sturdy is cultivated as permanent branch, and in the other rope bondage that the high waddy of 300cm is set with softness of the seedling of every strain grafting, guarantee that it is tree-like upright, in time remove lateral bud and side shoot on the permanent branch in the incubation, only stay the upwards growth of a terminal bud, treat that permanent branch is long to stay branch after during for 250cm; Grafting in mid-May, second permanent branch is carried out band girdle and lose money instead of making money skin, a stripping mouthful width is 2.5cm, late August once more band girdle lose money instead of making money skin, a stripping mouthful width is 3cm, the water and fertilizer management mode is identical with the general cultivation management method of oranges and tangerines.
Comparative Examples 3
Adopt the spring among the embodiment 2 to see tangerine orange seed, plant 50 strains in the field, its seeding row spacing carries out the routine plantation and cultivates by 2 * 3m.
Comparative Examples 4
Adopt the spring among the embodiment 2 to see tangerine orange seed, breeding method is routinely cultivated and is obtained the spring and see seedling 200 strains of tangerine orange, carries out conventional high position grafting and final-period management in early March, and wherein, the high position grafting interstock is selected 6 years living orange branches of anti-summer of trifoliate orange anvil honey for use.
Result of the test sees the following form 3:
Table 3 spring sees that the virgin phase of tangerine orange and corresponding strain number compare (unit: strain)
| Handle | Seedling | Survive seedling | 2 years virgin phases | 3 years virgin phases | 4 years virgin phases |
| Comparative Examples 3 | ??50 | ??42 | ??0 | ??0 | ??0 |
| Comparative Examples 4 | ??200 | ??191 | ??0 | ??0 | ??2 |
| Embodiment 2 | ??500 | ??490 | ??363 | ??127 |
As can be seen, the breeding method in Comparative Examples 3 and the Comparative Examples 4, the spring sees that the virgin phase of tangerine orange is very long, at least all takes 4 years, this method breeding cycle is long, inefficiency.
Embodiment 3-14
With following table 4 described 12 oranges and tangerines inter breed crossing seeds, adopt and embodiment 1 identical operations method, cultivate;
The virgin phase of 12 oranges and tangerines kinds and corresponding strain number (unit: strain) thereof among the table 4 embodiment 3-14
| Embodiment | The kind name | Hybridization seedling strain number | Survive seedling | 2 years virgin phases | 3 years virgin phases | Spent the virgin hundreds of proportions by subtraction of phase strain (%) in 2 years |
| ??3 | Xing Jin | ??40 | ??40 | ??38 | ??2 | ??95 |
| ??4 | The granulated sugar tangerine | ??22 | ??22 | ??22 | ??100 | |
| ??5 | Southern No. 1 of day | ??56 | ??55 | ??42 | ??13 | ??76 |
| ??6 | Like beautiful woman No. 28 | ??55 | ??55 | ??42 | ??13 | ??76 |
| ??7 | Like beautiful woman No. 38 | ??30 | ??30 | ??23 | ??7 | ??77 |
| ??8 | Like beautiful woman No. 21 | ??22 | ??22 | ??21 | ??1 | ??95 |
| ??9 | Like beautiful woman No. 22 | ??22 | ??22 | ??20 | ??2 | ??91 |
| ??10 | Like beautiful woman No. 30 | ??30 | ??30 | ??28 | ??2 | ??93 |
| ??11 | No. 32, Tianjin of mouth | ??30 | ??29 | ??27 | ??2 | ??93 |
| ??12 | Spring perfume (or spice) | ??30 | ??28 | ??23 | ??5 | ??82 |
| ??13 | The perfume (or spice) in Tianjin | ??40 | ??40 | ??28 | ??12 | ??70 |
| ??14 | Like beautiful woman No. 34 | ??60 | ??56 | ??55 | ??1 | ??98 |
Conclusion: by last table 4 as can be known, this method makes the virgin phase of the hybrid tangerine more than 70% shorten in 2 years, and all the other virgin phases are 3 years, thereby have accelerated the process of oranges and tangerines breedings, and do not see harmful effect in the experimentation.
Claims (4)
1. method that shortens juvenile period of hybrid tangerine is characterized in that:
The cultivation of its hybrid seedling is to cultivate to growing two true leaves and secondary root system;
Its high position grafting is performed such: earlier the root system below the hybrid seedling hypocotyl is pruned, remaining part is as a scion; Select with the scion affiliation closely, and do not have damage by disease and insect, more than the liftoff 100cm, the above oranges and tangerines branch of diameter 3cm is as interstock, in sowing then mid-February to grafting mid-March, its every interstock on grafting one strain scion; Place the sunshade of 45%～60% obscurity off the net afterwards, treat to separate when the new long terminal bud of scion or arbitrary lateral bud length reach 0.5～1.5cm film;
Its final-period management is performed such: after treating that scion grows young sprout, one tender shoots of selecting and remain the most sturdy is cultivated as permanent branch, and the traction thing is set guarantees that it is tree-like upright, in time remove lateral bud and side shoot on the permanent branch in the incubation, treat that permanent branch is long to stay branch after above for 200cm; Grafting in May, second early and middle ten days carries out band girdle to permanent branch and loses money instead of making money skin, late August once more band girdle lose money instead of making money skin.
2. the method for shortening juvenile period of hybrid tangerine as claimed in claim 1, it is characterized in that: the cultivation of described hybrid seedling, adopt such method to carry out: with the hybrid oranges and tangerines seed of maturation, peel off outer, interior two-layer kind of skin, no soil is cultured to seedling and grows two true leaves and secondary root system.
3. the method for shortening juvenile period of hybrid tangerine as claimed in claim 1 or 2 is characterized in that: during described high position grafting, it is the contact-making surface of 1～2cm that hybrid seedling is whittled into the face length of splaying from hypocotyl, and part that will whole strain seedling remainder is as a scion; When described band girdle was lost money instead of making money skin, a stripping mouthful width was 2～3cm.
4. the method for shortening juvenile period of hybrid tangerine as claimed in claim 1 or 2 is characterized in that: it is to be undertaken by such step:
1), the cultivation of hybrid seedling: the fully ripe hybrid oranges and tangerines seed of gathering, peel off outer, the interior two-layer kind of skin of seed, no earth culture is educated seedling in incubator; Its breeding method is: in the plastic culture case of 15 * 15 * 30cm, case heelpiece thickness is the aseptic filter paper of 10cm, after water-wet, keep having at the bottom of the case degree of depth to be no more than the water of 1cm, cultivate 100～150 in seed peelling off kind of skin in every case, indoor 25～30 ℃ of constant temperature, daylight 10 hours, evening, dark condition was cultivated down, and the case mouth is tightened with film and preserved moisture, and separated the film ventilation once in per 2～3 days; After treating that hybrid seedling grows two true leaves and secondary root system, be directly used in high position grafting;
2), high position grafting: earlier the root system below the hybrid seedling hypocotyl is pruned, being whittled into the face length of splaying at the hypocotyl place is the scion of 1～2cm; Select with the scion affiliation closely, and do not have damage by disease and insect, more than the liftoff 100cm, the above oranges and tangerines branch of diameter 3cm is as interstock, in sowing then mid-February to grafting mid-March, its every interstock on grafting one strain scion; Placing obscurity afterwards is that 45% sunshade is off the net, treats to separate when 0.5～1.5cm is grown in scion terminal bud or either side blastogenesis film;
3), final-period management: after treating that scion grows young sprout, the spray of selecting and remain the most sturdy is cultivated as permanent branch, and at the other traction thing that is provided with of the seedling of every strain grafting, guarantee that it is tree-like upright, in time remove lateral bud and side shoot on the permanent branch in the cultivation, only stay the upwards growth of a terminal bud, treat that the long 200cm of being of permanent branch stays branch after above; Grafting in May, second early and middle ten days carries out band girdle to permanent branch and loses money instead of making money skin, and a stripping mouthful width is 2～3cm, late August once more band girdle lose money instead of making money skin, a stripping mouthful width is 2～3cm, the water and fertilizer management mode is identical with the general cultivation management method of oranges and tangerines.
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| CN104170657A (en) * | 2014-08-29 | 2014-12-03 | 周景龙 | Citrus grafting method |
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| CN102986456A (en) * | 2012-12-14 | 2013-03-27 | 福建省农业科学院果树研究所 | Grafting method for shortening juvenile phase of hybrid progeny of longan |
| CN104160904A (en) * | 2014-07-22 | 2014-11-26 | 杨兆寅 | Method for transforming short-old tree low yield citrus orchard |
| CN104170657A (en) * | 2014-08-29 | 2014-12-03 | 周景龙 | Citrus grafting method |
| CN104663259A (en) * | 2015-02-06 | 2015-06-03 | 中国农业科学院柑桔研究所 | Grafting method for hybrid citrus seed seedlings |
| CN105359909A (en) * | 2015-10-20 | 2016-03-02 | 西南大学柑桔研究所 | Method for inducing vein burst of Newhall navel orange in magnesium deficiency condition |
| CN105594463B (en) * | 2016-01-15 | 2019-04-23 | 中国农业科学院柑桔研究所 | A kind of citrus Grafting of buds method |
| CN105594463A (en) * | 2016-01-15 | 2016-05-25 | 中国农业科学院柑桔研究所 | Method for grafting tender orange shoot |
| CN105660237A (en) * | 2016-01-29 | 2016-06-15 | 新疆农业科学院园艺作物研究所 | Method for shortening juvenile period of directive breeding hybrid seedlings of Pyrus bretschneideri Rehd. |
| CN105660237B (en) * | 2016-01-29 | 2018-08-17 | 新疆农业科学院园艺作物研究所 | A method of shortening Kuerle delicious pear directive breeding cross hybrid seedling juvenile phase |
| CN107258435A (en) * | 2017-06-27 | 2017-10-20 | 江西省农业科学院园艺研究所 | It is a kind of to promote citrus to do sth. in advance the method yielded positive results |
| CN107409776A (en) * | 2017-09-05 | 2017-12-01 | 福建省农业科学院果树研究所 | A kind of method for shortening citrus juvenile phase of hybrid progeny |
| CN108419627A (en) * | 2018-03-02 | 2018-08-21 | 成都市蒲江名特果业有限公司 | A kind of implantation methods of rake rake mandarin orange |
| CN114303669A (en) * | 2021-12-30 | 2022-04-12 | 湖北省农业科学院果树茶叶研究所 | Method for shortening childhood and childhood period of persimmon seedlings |
| CN116584258A (en) * | 2023-06-09 | 2023-08-15 | 浙江省柑橘研究所 | Citrus hybrid seed embryo grafting method |
| CN116584258B (en) * | 2023-06-09 | 2024-04-16 | 浙江省柑橘研究所 | Citrus hybrid seed embryo grafting method |
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