CN105594463A - Method for grafting tender orange shoot - Google Patents
Method for grafting tender orange shoot Download PDFInfo
- Publication number
- CN105594463A CN105594463A CN201610027041.9A CN201610027041A CN105594463A CN 105594463 A CN105594463 A CN 105594463A CN 201610027041 A CN201610027041 A CN 201610027041A CN 105594463 A CN105594463 A CN 105594463A
- Authority
- CN
- China
- Prior art keywords
- grafting
- oranges
- tangerines
- tender
- orange
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to the field of orange plantation and particularly relates to a method for grafting a tender orange shoot and application of the method. The method for grafting the tender orange shoot comprises the following steps of grafting the tender orange grafting shoot to the tender position at the top of an orange stock seedling, and then covering the grafting shoot with a conical plastic bushing. By means of the method, the problem that conventional grafting technologies cannot conduct field grafting with tender orange scions is effectively solved, and meanwhile, the problems that the test tube tiny-shoot grafting technology cannot be conducted in the field and cannot utilize old and ripe stocks are further solved. By means of the method, the tender orange shoot can be grafted to the old and ripe stock successfully for the first time, and the survival rate is approximately 100%.
Description
Technical field
The invention belongs to oranges and tangerines field of planting, be specifically related to a kind of oranges and tangerines Grafting of buds method and application thereof.
Background technology
Grafting is one of artificial asexual multiplication's method of plant. Branch or the bud of a plant, grafting, on the stem or root of another plant, makes two parts that are connected together grow up to a complete plant. Grafting some are not produced to seed bearing fruit tree (as persimmon, some kinds of oranges and tangerines) and the breeding meaning of improved seeds great. Grafting can keep the merit of scion variety, can utilize again the advantageous feature of stock, reaches precocity fruiting, strengthens the ability of winter resistance, drought resistance, disease and insect resistance etc., can also economic utilization propagating materials, increase nursery stock quantity. Be usually used in the breeding of fruit tree, forest, flowers; Also grow seedlings for melon vegetables. On oranges and tangerines, general field graft technology can only utilize the scion branch aging, degree of lignification is higher to carry out grafting, can not utilize tender, the not aging scion branch of children to carry out grafting, otherwise its survival rate is zero. Although the Shoot-tip Grafting of cultivating in test tube or gemmule grafting can utilize very young tender scion to carry out grafting, it requires stock children tender, and can only in test tube, cultivate. For the grafting of oranges and tangerines tender shoots, because oranges and tangerines tender shoots is that oranges and tangerines plant sprouts young sprout soon, now young sprout is not yet aging, and degree of lignification is very low, can not realize well the grafting of oranges and tangerines tender shoots.
As can be seen here, can invent a kind of oranges and tangerines Grafting of buds method, solve conventional graft technology and can not utilize the problem of young tender scion, become those skilled in the art's technical barrier urgently to be resolved hurrily.
Summary of the invention
In order to overcome existing problem in prior art, the object of the present invention is to provide a kind of oranges and tangerines Grafting of buds method and application thereof.
To achieve these goals and other relevant objects, the present invention adopts following technical scheme:
A first aspect of the present invention, provides a kind of oranges and tangerines Grafting of buds method, has comprised following steps: to You Nen position, rootstocks for citrus seedling top, then adopt conical plastics sleeve pipe to cover grafting bud the tender oranges and tangerines children grafting of buddering.
Preferably, described conical plastics sleeve pipe comprises bottom end opening and top end opening, and the diameter of described bottom end opening is greater than the diameter of described top end opening.
Preferably, the diameter range of described bottom end opening is 4～6mm. More preferably, the diameter range of described bottom end opening is 5mm.
Preferably, the diameter range of described top end opening is 1～3mm. Further preferably, the diameter range of described top end opening is 2mm.
Preferably, the material of described conical plastics sleeve pipe is PP material.
Preferably, described conical plastics sleeve pipe is transparent plastic casing.
Preferably, the color of described conical plastics sleeve pipe is selected from any in white, yellow or blueness.
Preferably, tender the buddering of described oranges and tangerines children refers to the bud of sprouting rear ten days to 30 days.
Preferably, You Nen position, described rootstocks for citrus seedling top refers to that top sprouts after young sprout 30 days with interior tender taper position.
Preferably, described rootstocks for citrus seedling is selected from any in Ka Lizuo citrange rootstock seedling, trifoliate orange rootstock seedling, fragrant citrus rootstock seedling or red tangerine rootstock seedling.
Preferably, described rootstocks for citrus seedling is selected from but the rootstocks for citrus seedling that is not limited to grow directly from seeds.
A second aspect of the present invention, provides the application of aforementioned oranges and tangerines Grafting of buds method in oranges and tangerines field of planting.
Compared with prior art, the present invention has following beneficial effect:
Method of the present invention efficiently solves the problem that conventional graft technology can not utilize the tender scion of oranges and tangerines children to carry out field grafting, and meanwhile also having solved test tube gemmule graft technology can not implement in field and the problem that can not utilize aging stock; Method of the present invention has realized the successful grafting on aging stock of oranges and tangerines tender shoots first, and survival rate nearly 100%.
Detailed description of the invention
Before further describing the specific embodiment of the invention, should be understood that protection scope of the present invention is not limited to following specific specific embodiments; It is also understood that the term using in the embodiment of the present invention is in order to describe specific specific embodiments, instead of in order to limit the scope of the invention. The test method of unreceipted actual conditions in the following example, conventionally according to normal condition, or the condition of advising according to each manufacturer.
In the time that embodiment provides number range, unless should be understood that the present invention is otherwise noted, between two end points of each number range and two end points, any one numerical value all can be selected. Unless otherwise defined, all technology that use in the present invention are identical with the meaning that those skilled in the art of the present technique understand conventionally with scientific terminology. The concrete grammar that uses in embodiment, equipment, material, grasp according to those skilled in the art to prior art and record of the present invention, can also realize the present invention with any method, equipment and material similar to the method described in the embodiment of the present invention, equipment, material or prior art that be equal to.
Unless otherwise indicated, in the present invention, disclosed experimental technique, detection method, preparation method all adopt the biology, biochemistry, analytical chemistry, cell of the art routine to cultivate and the routine techniques of association area. These technology are existing perfect explanation in existing document, specifically can be referring to MOLECULARCLONING:ALABORATORYMANUAL such as Sambrook, Secondedition, ColdSpringHarborLaboratoryPress, 1989andThirdedition, 2001; Ausubel etc., CURRENTPROTOCOLSINMOLECULARBIOLOGY, john wiley & sons, NewYork, 1987andperiodicupdates; TheseriesMETHODSINENZYMOLOGY, AcademicPress, SanDiego; Wolffe, CHROMATINSTRUCTUREANDFUNCTION, Thirdedition, AcademicPress, SanDiego, 1998; METHODSINENZYMOLOGY, Vol.304, Chromatin (P.M.WassarmanandA.P.Wolffe, eds.), AcademicPress, SanDiego, 1999; And METHODSINMOLECULARBIOLOGY, Vol.119, ChromatinProtocols (P.B.Becker, ed.) HumanaPress, Totowa, 1999 etc.
Embodiment 1
1. experiment material
Ka Lizuo citrange rootstock seedling 60 strains of growing directly from seeds above for 1 year that cultivate in field; The sweet orange of not aging (children is tender, refers to the bud of sprouting rear ten days to 30 days) is buddered 60.
2. experimental technique
2 processed group are established in experiment.
Processed group 1: get grow directly from seeds Ka Lizuo citrange rootstock seedling and 30 tender sweet oranges of children of 30 strains and budder, the selection You Nen position, Ka Lizuo citrange rootstock seedling top (refer to top sprout after young sprout 30 days with interior tender taper position) of growing directly from seeds, in cut-grafting mode, tender children sweet orange is buddered to grafting to the Ka Lizuo citrange rootstock seedling branch top end that grows directly from seeds, then selecting sizeable conical plastics sleeve pipe to cover the tender sweet orange of the children who grafts budders, described conical plastics sleeve pipe comprises bottom end opening and top end opening, and the diameter of described bottom end opening is greater than the diameter of described top end opening. Generally, the diameter range of described bottom end opening is 4～6mm. The diameter range of described top end opening is 1～3mm. The size cases that can budder according to rootstock seedling and young tender sweet orange, is applicable to big or small conical plastics sleeve pipe being selected from. Conical plastics sleeve pipe generally adopts environmental protection PP material to make. Alternative color adularescent, yellow and blue etc.
Processed group 2: get grow directly from seeds Ka Lizuo citrange rootstock seedling and 30 tender sweet oranges of children of 30 strains and budder, high 5 centimetres of positions are shown from soil in the selection Ka Lizuo citrange rootstock seedling limb bottom of growing directly from seeds, in traditional side grafting mode, tender children sweet orange is buddered to grafting to the limb place, Ka Lizuo citrange rootstock seedling bottom of growing directly from seeds, then budder with the tender sweet orange of the good children of plastic sheeting binding grafting.
Whether the seedling that above-mentioned 2 kinds of processed group gained are grafted is placed under the equivalent environment of field and carries out follow-up cultivation, within 15 days, observe afterwards the sweet orange tender shoots grafting and survive.
3. results and analysis
Experiment was processed after 15 days, took off the conical plastics sleeve pipe on the seedling of processed group 1, checked that the sweet orange tender shoots of seedling top You Nen position grafting survives situation, find that the tender shoots on 30 young plants all survives, tender shoots does not show brown stain, is still green, that is survival rate is 100%. Remove the plastic sheeting on the seedling of processing 2 simultaneously, check that the sweet orange tender shoots of seedling bottom grafting survives situation, find that the tender shoots on 30 young plants is all dead, the whole brown stains of tender shoots. The method of presentation of results processed group 1, i.e. top You Nen position grafting children tender shoots can be realized the tender scion of grafting children on the rootstock seedling of field, and traditional grafting method, processes 2 graftings that can not continue young tender scion.
Conclusion and discussion
The oranges and tangerines Grafting of buds method that the present invention sets up be first by the tender grafting of buddering of oranges and tangerines childrens to the You Nen position, Ka Lizuo citrange rootstock seedling top of growing directly from seeds, then adopt conical plastics sleeve pipe to cover grafting bud, sprout and stock are fitted tightly. Grafting is carried out at the selection tender top of Ka Lizuo citrange rootstock seedling children of growing directly from seeds, could ensure oranges and tangerines tender shoots can be fine and stock heal, be convenient to conical plastics shroud simultaneously and live grafting bud; Grafting is for example, by conical plastics sleeve pipe material (PP material) waterproof, has ensured the not dehydration of grafting bud, avoids thirsting; The both ends open two of plastic bushing all has good gas permeability, while avoiding grafting bud to meet sun direct projection, is suffocated, and realizes thus oranges and tangerines tender shoots and can successfully be grafted on the stock of cultivating in field. The experiment later stage has also been attempted other various rootstocks for citrus kinds (for example: trifoliate orange rootstock seedling, fragrant citrus rootstock seedling or red tangerine rootstock seedling) and other various oranges and tangerines scion varieties, adopts tender shoots of the present invention top grafting method all can reach the tender shoots survival rate that is close to 100%.
The above; it is only preferred embodiment of the present invention; not to any formal and substantial restriction of the present invention; should be understood that; for those skilled in the art; do not departing under the prerequisite of the inventive method, also can make some improvement and supplement, these improvement and the supplementary protection scope of the present invention that also should be considered as. All those skilled in the art, without departing from the spirit and scope of the present invention, a little variation of making when utilizing disclosed above technology contents, the equivalent variations of modifying and developing, be equivalent embodiment of the present invention; Meanwhile, the change of any equivalent variations that all foundations essence technology of the present invention is done above-described embodiment, modification and differentiation, all still belong in the scope of technical scheme of the present invention.
Claims (10)
1. an oranges and tangerines Grafting of buds method, has comprised following steps: the tender oranges and tangerines children grafting of buddering is arrived to rootstocks for citrus seedling top childrenTender position, then adopts conical plastics sleeve pipe to cover grafting bud.
2. oranges and tangerines Grafting of buds method according to claim 1, is characterized in that, described conical plastics sleeve pipe comprises that bottom opensMouth and top end opening, the diameter of described bottom end opening is greater than the diameter of described top end opening.
3. oranges and tangerines Grafting of buds method according to claim 2, is characterized in that, the diameter range of described bottom end opening is 4～6mm。
4. oranges and tangerines Grafting of buds method according to claim 2, is characterized in that, the diameter range of described top end opening is 1～3mm。
5. oranges and tangerines Grafting of buds method according to claim 1, is characterized in that, the material of described conical plastics sleeve pipe isPP material.
6. oranges and tangerines Grafting of buds method according to claim 1, is characterized in that, described conical plastics sleeve pipe is transparent mouldingMaterial sleeve pipe.
7. oranges and tangerines Grafting of buds method according to claim 1, is characterized in that, the color of described conical plastics sleeve pipe is selected fromAny in white, yellow or blueness.
8. oranges and tangerines Grafting of buds method according to claim 1, is characterized in that, described rootstocks for citrus seedling is selected from Ka Lizuo trifoliate orangeAny in orange rootstock seedling, trifoliate orange rootstock seedling, fragrant citrus rootstock seedling or red tangerine rootstock seedling.
9. oranges and tangerines Grafting of buds method according to claim 1, is characterized in that, described rootstocks for citrus seedling is the oranges and tangerines anvil that grows directly from seedsWood seedling.
10. the application of oranges and tangerines Grafting of buds method in oranges and tangerines field of planting as described in claim as arbitrary in claim 1～9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610027041.9A CN105594463B (en) | 2016-01-15 | 2016-01-15 | A kind of citrus Grafting of buds method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610027041.9A CN105594463B (en) | 2016-01-15 | 2016-01-15 | A kind of citrus Grafting of buds method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105594463A true CN105594463A (en) | 2016-05-25 |
| CN105594463B CN105594463B (en) | 2019-04-23 |
Family
ID=55975285
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610027041.9A Expired - Fee Related CN105594463B (en) | 2016-01-15 | 2016-01-15 | A kind of citrus Grafting of buds method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105594463B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107646460A (en) * | 2016-07-25 | 2018-02-02 | 乐山市柠香果农业开发有限公司 | A kind of breeding method of lemon tangerine |
| CN108029431A (en) * | 2017-12-26 | 2018-05-15 | 建水县鼎盛农业发展有限公司 | Citrus grafting seedlings-growing method |
| CN110199699A (en) * | 2019-07-03 | 2019-09-06 | 西南大学 | Method that is a kind of while improving Grafted Citrus survival rate and germination rate |
| CN110337933A (en) * | 2019-08-22 | 2019-10-18 | 重庆俊瑞农业综合开发有限公司 | A method of stock grafting lemon is cooked with Fructus Aurantii |
| CN114766359A (en) * | 2022-04-22 | 2022-07-22 | 华中农业大学 | Test-tube plantlet support and application thereof in micro-bud grafting detoxification of citrus stem tips |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101884294A (en) * | 2010-06-25 | 2010-11-17 | 西南大学柑桔研究所 | Method for reducing juvenile period of hybrid tangerine |
| CN102119642A (en) * | 2010-12-16 | 2011-07-13 | 宜昌市科力生实业有限公司 | Method for grafting citrus sprout by using citrange cuttage stock |
| CN102783353A (en) * | 2012-05-31 | 2012-11-21 | 曹帮华 | Method for grafting, cutting and fast propagating Chinese white poplar twig |
| CN102919061A (en) * | 2012-11-21 | 2013-02-13 | 贵州省亚热带作物研究所 | Mango grafting method using green braches with leaves |
| CN104663259A (en) * | 2015-02-06 | 2015-06-03 | 中国农业科学院柑桔研究所 | Grafting method for hybrid citrus seed seedlings |
| CN104813851A (en) * | 2015-04-28 | 2015-08-05 | 浙江伟达园林工程有限公司 | Method for improving seedling grafting survival rate |
-
2016
- 2016-01-15 CN CN201610027041.9A patent/CN105594463B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101884294A (en) * | 2010-06-25 | 2010-11-17 | 西南大学柑桔研究所 | Method for reducing juvenile period of hybrid tangerine |
| CN102119642A (en) * | 2010-12-16 | 2011-07-13 | 宜昌市科力生实业有限公司 | Method for grafting citrus sprout by using citrange cuttage stock |
| CN102783353A (en) * | 2012-05-31 | 2012-11-21 | 曹帮华 | Method for grafting, cutting and fast propagating Chinese white poplar twig |
| CN102919061A (en) * | 2012-11-21 | 2013-02-13 | 贵州省亚热带作物研究所 | Mango grafting method using green braches with leaves |
| CN104663259A (en) * | 2015-02-06 | 2015-06-03 | 中国农业科学院柑桔研究所 | Grafting method for hybrid citrus seed seedlings |
| CN104813851A (en) * | 2015-04-28 | 2015-08-05 | 浙江伟达园林工程有限公司 | Method for improving seedling grafting survival rate |
Non-Patent Citations (2)
| Title |
|---|
| 张元国: "《蔬菜集约化育苗技术》", 31 July 2014, 北京金盾出版社 * |
| 陈泽雄等: "柑橘温室嫩芽嫁接技术初探", 《林业实用技术》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107646460A (en) * | 2016-07-25 | 2018-02-02 | 乐山市柠香果农业开发有限公司 | A kind of breeding method of lemon tangerine |
| CN108029431A (en) * | 2017-12-26 | 2018-05-15 | 建水县鼎盛农业发展有限公司 | Citrus grafting seedlings-growing method |
| CN110199699A (en) * | 2019-07-03 | 2019-09-06 | 西南大学 | Method that is a kind of while improving Grafted Citrus survival rate and germination rate |
| CN110337933A (en) * | 2019-08-22 | 2019-10-18 | 重庆俊瑞农业综合开发有限公司 | A method of stock grafting lemon is cooked with Fructus Aurantii |
| CN114766359A (en) * | 2022-04-22 | 2022-07-22 | 华中农业大学 | Test-tube plantlet support and application thereof in micro-bud grafting detoxification of citrus stem tips |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105594463B (en) | 2019-04-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2015338670B2 (en) | Methods for providing height enhanced grafted plants and products thereof | |
| Mohamed et al. | Evaluation of different grafting methods and rootstocks in watermelon grown in Egypt | |
| CN105594463A (en) | Method for grafting tender orange shoot | |
| Tirupathamma et al. | Vegetable grafting: A multiple crop improvement methodology | |
| Gotur et al. | Performance of wedge grafting in guava (Psidium guajava L.) under different growing conditions | |
| CN106332661A (en) | Method of grafting liquidambar styraciflua | |
| CN105917979A (en) | Operation method for grafting peonies | |
| Dolgun et al. | A histological investigation on graft formation of some nectarine cultivars grafted on pixy rootstock | |
| Jat et al. | Hybrid seed production of bitter gourd is a remunerative venture | |
| Priadi et al. | Effects of Compost Type and Rootstock Length on Fruit and Vegetable Seedlings Growth in the Nursery | |
| Mohamed et al. | A method of rapid propagation of hot pepper using marcotting technique to maintain clonal characteristics | |
| Van Hooijdonk et al. | Leaf area development and dry matter production of newly grafted monoaxis and biaxis apple trees | |
| Salazar et al. | Propagation and bulblet enhancement of Rhodophiala pratensis from seeds germinated in vitro | |
| Yang et al. | Effects of intercropping with post-grafting generation of Impatiens balsamina on potassium uptake of grape seedlings under cadmium stress | |
| Vidyadhar et al. | Effect of growing conditions on growth, seed yield and quality attributes in cherry tomato (Solanum lycopersicum var cerasiferme) | |
| Muna | Standardization of Media Combinations for Hardening of Orchid Dendrobium Spp Cv. Sonia-17 | |
| CN107360962A (en) | A kind of golden-rimmed thorny elaeagnus efficient crossbreeding method | |
| Gotur et al. | Effect of Scion Cultivar and Grafting Season on Success of Wedge Grafting in Guava (Psidium guajava L.) | |
| Stewart | Tolerance of Two Grafted Tomato Plants (F1 Mongal and Buru Buru; Calinago F1 and Buru Buru) to Fungal Diseases | |
| Sudhersan et al. | Introduction, evaluation and propagation of Ziziphus In Kuwait | |
| Gilbert | SHORT NOTE Air layering as a propagation method in glasshouse cultivation | |
| Đurić et al. | Hazel cultivation in Bosnia and Herzegovina | |
| Kumar | Planting material production technology in seed propagated perennial spices–Clove, allspice and curry leaf | |
| Ismeet Singh et al. | Effect of pruning systems on growth and yield traits of greenhouse grown bell pepper (Capsicum annuum L. var. grossum). | |
| Yakandawala et al. | Osbeckia octandra: A potential shrub for urban environment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190423 Termination date: 20200115 |