CN101426918A - 磷脂酶,编码磷脂酶的核酸以及制备和应用磷脂酶的方法 - Google Patents
磷脂酶,编码磷脂酶的核酸以及制备和应用磷脂酶的方法 Download PDFInfo
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Abstract
Description
SEQ IDNO: | 酶类型 | 信号序列位置(AA=氨基酸) | 信号(AA) | 来源 |
143,144 | PA-特异性PLA | 未知 | ||
25,26 | Patatin | 未知 | ||
77,78 | Patatin | 未 |
知 | ||||
35,36 | Patatin | 未知 | ||
125,126 | Patatin | 未知 | ||
135,136 | Patatin | 未知 | ||
99,100 | Patatin | 未知 | ||
65,66 | Patatin | 未知 | ||
87,88 | Patatin | 未知 | ||
86,87 | Patatin | 未知 | ||
45,46 | Patatin | 未知 | ||
59,60 | Patatin | 未知 | ||
13,14 | Patatin | 未知 | ||
71,72 | Patatin | 未知 | ||
55,56 | Patatin | 未知 | ||
33,34 | Patatin | 未知 | ||
91,92 | Patatin | 未知 | ||
103,104 | Patatin | 未知 | ||
11,12 | Patatin | 未知 | ||
17,18 | Patatin | 未知 |
95,96 | Patatin | 未知 | ||
43,44 | Patatin | 未知 | ||
27,28 | Patatin | 未知 | ||
131,132 | Patatin | 未知 | ||
127,128 | Patatin | 未知 | ||
133,134 | Patatin | 未知 | ||
137,138 | Patatin | 未知 | ||
165,166 | Patatin | 未知 | ||
167,168 | 磷脂酸磷酸酶 | AA1-30 | MARSWKWRPLLSSFLLVSLAPFSTSVPCFK | 未知 |
169,170 | 磷脂酸磷酸酶 | 未知 | ||
171,172 | 磷脂酸磷酸酶 | 未知 | ||
173,174 | 磷脂酸磷酸酶 | 未知 | ||
111,112 | 磷脂酰肌醇PLC | AA1-16 | MGAGAILLTGAPTASA | 细菌 |
107,108 | 磷脂酰肌醇PLC | AA1-23 | MSNKKFILKLFICSTILSTFVFA | 未知 |
109,110 | 磷脂酰肌醇PLC | AA1-23 | MSNKKFILKLFICSTILSTFVFA | 未知 |
113,114 | 磷脂酰肌醇PLC | AA1-23 | MSNKKFILKLFICSTILSTFVFA | 未知 |
117,118 | 磷脂酰肌醇PLC | AA1-23 | MNNKKFILKLFICSMVLSAFVFA | 未知 |
119,120 | 磷脂酰肌醇PLC | AA1-23 | MNNKKFILKLFICSMVLSAFVFA | 未知 |
醇PLC | 知 | |||
115,116 | 磷脂酰肌醇PLC | AA1-23 | MNNKKFILKLFICSMVLSAFVFA | 未知 |
121,122 | 磷脂酰肌醇PLC | AA1-23 | MRNKKFILKLLICSTVLSTFVFA | 未知 |
141,142 | 磷脂酶 | 未知 | ||
155,156 | 磷脂酶 | AA1-36 | MRTTTTNWRQIVKSLKLFLMGLCLFISASFASSAYA | 未知 |
159,160 | 磷脂酶 | 未知 | ||
145,146 | PLA | 未知 | ||
147,148 | PLA | 未知 | ||
149,150 | PLA | 未知 | ||
151,152 | PLA | 未知 | ||
153,154 | PLA | 未知 | ||
157,158 | PLA | 未知 | ||
163,164 | PLA | 未知 | ||
101,102 | PLC | AA1-39 | LSLVASLRRAPGAALALALAAATLAVTAQGATAAPAAAAA | 细菌 |
1,2 | PLC | AA1-24 | MKKKVLALAAMVALAAPVQSVVFAQ | 未知 |
3,4 | PLC | AA1-24 | MKRKILAIASVIALTAPIQSVAFAH | 未知 |
5,6 | PLC | AA1-24 | MKRKILAIASVIALTAPIQSVAFAH | 未知 |
97,98 | PLC | AA1-25 | MKRKLCTWALVTAIASSTAVIPTAAE | 未知 |
7,8 | PLC | AA1-29 | MITLIKKCLLVLTMTLLLGVFVPLQPSHAT | 未知 |
31,32 | PLC | AA1-20 | MKKKLCTWALVTAISSGWAI | 未知 |
81,82 | PLC | AA1-25 | MKKKLCTMALVTAISSGVVTIPTEAQ | 未知 |
93,94 | PLC | AA1-29 | MITLIKKCLLVLTMTLLSGVFVPLQPSYAT | 未知 |
89,90 | PLC | AA1-25 | MKKKLCTLAFVTAISSIAITIPTEAQ | 未知 |
123,124 | PLC | AA1-24 | MKKKVLALAAMVALAAPVQSVVFA | 未知 |
129,130 | PLC | AA1-27 | MKKKICTLALVSAITSGVVTIPTVASA | 未知 |
139,140 | PLC | AA1-20 | MKIKPLTFSFGLAVTSSVQA | 未知 |
105,106 | PLC | AA1-30 | MNRCRNSLNLQLRAVTVAALVVVASSAALAW | 未知 |
9,10 | PLC | AA1-20 | MKLLRVFVCVFALLSAHSKAD | 未知 |
47,48 | PLD | 未知 | ||
15,16 | PLD | 未知 | ||
41,42 | PLD | 未知 | ||
23,24 | PLD | 未知 | ||
51,52 | PLD | 未知 | ||
53,54 | PLD | 未知 | ||
19,20 | PLD | AA1-19 | MKKTTLVLALLMPFGAASAQ | 未知 |
75,76 | PLD | 未 |
知 | ||||
57,58 | PLD | 未知 | ||
63,64 | PLD | AA1-18 | MKNTLILAGCILAAPAVAD | 未知 |
79,80 | PLD | AA1-23 | MRNFSKGLTSILLSIATSTSAMAF | 未知 |
37,38 | PLD | AA1-23 | MRNFSKGLTSILLSIATSTSAMAF | 未知 |
61,62 | PLD | AA1-21 | MTLKLSLLIASLSAVSPAVLAN | 未知 |
67,68 | PLD | 无 | 未知 | |
83,84 | PLD | AA1-21 | MKKIVIYSFVAGVMTSGGVFAA | 未知 |
49,50 | PLD | AA1-23 | MNFWSFLLSITLPMGVGVAHAQPD | 未知 |
39,40 | PLD | 未知 | ||
73,74 | PLD | 未知 | ||
29,30 | PLD | 未知 | ||
21,22 | PLD | AA1-28 | MQQHKLRNFNKGLTGVVLSVLTSTSAMAF | 未知 |
71,72 | PLD | 未知 | ||
161,162 | PLD | AA1-24 | MNRKLLSLCLGATSCIALSLPVHA | 未知 |
碱炼助剂 | 脱胶助剂 | |
1)胶形成和分离中的油损失2.1% | X | X |
2)在加入苛性碱中皂化的油3.1% | X | |
3)在漂白中黏土中捕获的油*<1.0% | X | X |
总产量损失 ~5.2% | ~2.1% | ~5.2% |
碱炼助剂 | 脱胶助剂 | |
豆油/卵磷脂产生 | X | |
化学精炼的豆油、向日葵油、油菜油 | X | X |
低磷脂油(如,棕榈) | X |
共喂料速度(ml/min) | 诱导之前/之后的碳源 | 诱导OD | PLC活性(U/mlsup) | 消耗的MeOH(L) | Bodipy蛋白酶 | 最终OD |
0.51.522.53 | Glyc/GlycGlyc/GlycGlyc/GlycGlyc/GlycGlyc/Glyc | 250-300 | 1001100155015501715 | 11.71.31.41.4 | 是否否否否 | 450680860900820 |
Mut | 共喂料速度(ml/min) | 诱导OD | PLC活性(U/mlsup) | 消耗的MeOH(L) | Bodipy蛋白酶 | 最终OD |
S | 0.51.522.53 | 250-300 | 1001100155015501715 | 11.71.31.41.4 | 是否否否否 | 450680860900820 |
+ | 0.50.51111.51.51.52 | 250-300 | 100112001786201017682669269325972154 | 5.675.96.87.9107.18.18.3 | 是否否否否否否否否 | 871908988930700701818804752 |
Claims (277)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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US10/796,907 US7226771B2 (en) | 2002-04-19 | 2004-03-08 | Phospholipases, nucleic acids encoding them and methods for making and using them |
US10/796,907 | 2004-03-08 | ||
PCT/US2005/007908 WO2005086900A2 (en) | 2004-03-08 | 2005-03-08 | Phospholipases, nucleic acids encoding them and methods for making and using them |
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CN101426918A true CN101426918A (zh) | 2009-05-06 |
CN101426918B CN101426918B (zh) | 2013-03-27 |
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CN2005800146676A Active CN101426918B (zh) | 2004-03-08 | 2005-03-08 | 磷脂酶,编码磷脂酶的核酸以及制备和应用磷脂酶的方法 |
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US (2) | US7226771B2 (zh) |
EP (2) | EP1748954B1 (zh) |
JP (2) | JP2007531516A (zh) |
CN (1) | CN101426918B (zh) |
AU (1) | AU2005221136B2 (zh) |
BR (1) | BRPI0508559B1 (zh) |
CA (1) | CA2559060C (zh) |
ES (1) | ES2601431T3 (zh) |
HU (1) | HUE030573T2 (zh) |
PL (1) | PL1748954T3 (zh) |
PT (1) | PT1748954T (zh) |
WO (1) | WO2005086900A2 (zh) |
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US7977080B2 (en) | 2011-07-12 |
US7226771B2 (en) | 2007-06-05 |
US20050108789A1 (en) | 2005-05-19 |
JP2007531516A (ja) | 2007-11-08 |
ES2601431T3 (es) | 2017-02-15 |
CA2559060C (en) | 2017-09-05 |
CA2559060A1 (en) | 2005-09-22 |
AU2005221136B2 (en) | 2011-06-09 |
EP3190182A1 (en) | 2017-07-12 |
PL1748954T3 (pl) | 2017-02-28 |
EP1748954A2 (en) | 2007-02-07 |
EP1748954A4 (en) | 2009-10-28 |
WO2005086900A2 (en) | 2005-09-22 |
AU2005221136A1 (en) | 2005-09-22 |
BRPI0508559A (pt) | 2007-08-14 |
CN101426918B (zh) | 2013-03-27 |
EP1748954B1 (en) | 2016-08-17 |
US20080317731A1 (en) | 2008-12-25 |
BRPI0508559B1 (pt) | 2019-12-10 |
PT1748954T (pt) | 2016-11-21 |
JP2011172583A (ja) | 2011-09-08 |
HUE030573T2 (en) | 2017-05-29 |
WO2005086900A3 (en) | 2009-04-02 |
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