CN101384705A - 用于肝祖细胞扩增或分化的细胞外基质组分 - Google Patents

用于肝祖细胞扩增或分化的细胞外基质组分 Download PDF

Info

Publication number
CN101384705A
CN101384705A CNA2006800509212A CN200680050921A CN101384705A CN 101384705 A CN101384705 A CN 101384705A CN A2006800509212 A CNA2006800509212 A CN A2006800509212A CN 200680050921 A CN200680050921 A CN 200680050921A CN 101384705 A CN101384705 A CN 101384705A
Authority
CN
China
Prior art keywords
cells
cell
progenitor cells
liver
hepatic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CNA2006800509212A
Other languages
English (en)
Chinese (zh)
Inventor
兰德尔·E·麦克莱兰
洛拉·M·里德
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of North Carolina at Chapel Hill
Original Assignee
University of North Carolina at Chapel Hill
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of North Carolina at Chapel Hill filed Critical University of North Carolina at Chapel Hill
Publication of CN101384705A publication Critical patent/CN101384705A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0672—Stem cells; Progenitor cells; Precursor cells; Oval cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/02—Coculture with; Conditioned medium produced by embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/03—Coculture with; Conditioned medium produced by non-embryonic pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/14—Coculture with; Conditioned medium produced by hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
CNA2006800509212A 2005-11-16 2006-11-15 用于肝祖细胞扩增或分化的细胞外基质组分 Pending CN101384705A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US73687305P 2005-11-16 2005-11-16
US60/736,873 2005-11-16

Publications (1)

Publication Number Publication Date
CN101384705A true CN101384705A (zh) 2009-03-11

Family

ID=38049393

Family Applications (1)

Application Number Title Priority Date Filing Date
CNA2006800509212A Pending CN101384705A (zh) 2005-11-16 2006-11-15 用于肝祖细胞扩增或分化的细胞外基质组分

Country Status (13)

Country Link
US (2) US20070155009A1 (https=)
EP (1) EP1954804A4 (https=)
JP (1) JP2009515558A (https=)
KR (1) KR20080097985A (https=)
CN (1) CN101384705A (https=)
AU (1) AU2006315163A1 (https=)
BR (1) BRPI0618724A2 (https=)
CA (1) CA2630355A1 (https=)
CR (1) CR10073A (https=)
IL (1) IL191533A0 (https=)
RU (1) RU2008124158A (https=)
TW (1) TW200730627A (https=)
WO (1) WO2007059501A2 (https=)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102985130A (zh) * 2010-05-07 2013-03-20 北卡罗来纳大学查伯山分校 移植来自实体组织的细胞的方法
CN105189738A (zh) * 2013-04-08 2015-12-23 国立研究开发法人医药基盘·健康·营养研究所 肝母细胞样细胞的培养方法及其培养物
CN106434532A (zh) * 2016-12-22 2017-02-22 叶宗耀 一种培养肝细胞的培养基及其制备方法
CN108431209A (zh) * 2015-06-12 2018-08-21 新加坡科技研究局 肝脏干细胞的衍生和成熟肝细胞类型及其用途
CN115948331A (zh) * 2022-10-17 2023-04-11 中国人民解放军西部战区总医院 一种从小鼠胆囊中分离获取间充质干细胞的方法

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020045260A1 (en) * 2000-10-17 2002-04-18 Shih-Chieh Hung Method of isolating mesenchymal stem cells
US8084023B2 (en) 2007-01-22 2011-12-27 The Board Of Trustees Of The University Of Arkansas Maintenance and propagation of mesenchymal stem cells
CA2680254A1 (en) * 2007-03-06 2008-09-12 University Of North Carolina At Chapel Hill Complexes of hyaluronans, other matrix components, hormones and growth factors for maintenance, expansion and/or differentiation of hepatic cells
AU2008266992A1 (en) * 2007-06-15 2008-12-24 University Of North Carolina At Chapel Hill Paracrine signals from mesenchymal feeder cells and regulating expansion and differentiation of hepatic progenitors using same
JP2010534065A (ja) * 2007-07-20 2010-11-04 セルアーティス アーベー ヒト胚盤胞幹細胞に、胚体内胚葉（ＤＥ‐ｈｅｐ）を介して由来する新規な肝細胞の集団
US8372643B2 (en) * 2007-12-06 2013-02-12 Agency For Science, Technology And Research Method for extracellular matrix mediated differentiation and proliferation of stem cells
RU2010152354A (ru) * 2008-05-22 2012-06-27 Веста Терапьютикс, Инк. (Us) Способ дифференцировки клеток-предшественников млекопитающих в инсулин-продуцирующие островковые клетки поджелудочной железы
US8298584B2 (en) * 2008-12-30 2012-10-30 Collagen Matrix, Inc. Biopolymeric membrane for wound protection and repair
US20100227393A1 (en) * 2009-03-06 2010-09-09 Eric Lagasse Liver stem cells: isolation of hepatic progenitor cells from the human gall bladder
CA3169831C (en) * 2009-10-30 2025-11-18 Sapienza Universita Di Roma Pluripodent Stem Cells from the Extrahepatic Biliary Tree and Methods for Their Isolation
WO2011077894A1 (ja) * 2009-12-25 2011-06-30 Ｓｃｉｖａｘ株式会社 スフェロイド形成促進方法およびスフェロイド形成促進培地
JPWO2011102532A1 (ja) * 2010-02-16 2013-06-17 国立大学法人九州大学 誘導肝細胞
CA2810444A1 (en) 2010-09-07 2012-03-15 The Board Of Regents Of The University Of Texas System Tissue-specific differentiation matrices and uses thereof
WO2012064606A2 (en) * 2010-11-10 2012-05-18 Wake Forest University Health Sciences Tissue-specific extracellular matrix with or without tissue protein components for cell culture
BR112015010256A2 (pt) * 2012-11-08 2017-07-11 Arthrodynamic Tech Animal Health Division Inc meio para cultivar, preservar e administrar células regenerativas
US9533013B2 (en) 2013-03-13 2017-01-03 University Of North Carolina At Chapel Hill Method of treating pancreatic and liver conditions by endoscopic-mediated (or laparoscopic-mediated) transplantation of stem cells into/onto bile duct walls of particular regions of the biliary tree
JP6278324B2 (ja) * 2014-07-16 2018-02-21 国立大学法人大阪大学 ラミニンフラグメントの細胞培養基質活性増強方法
JP6755085B2 (ja) * 2015-11-10 2020-09-16 日機装株式会社 細胞支持複合体および細胞支持複合体の製造方法
WO2017082026A1 (ja) * 2015-11-10 2017-05-18 日機装株式会社 細胞支持複合体および細胞支持複合体の製造方法
RU2618989C1 (ru) * 2016-03-09 2017-05-11 Федеральное государственное бюджетное образовательное учреждение высшего образования "Московский государственный университет имени М.В. Ломоносова" (МГУ) Способ лечения печеночной недостаточности
US20170369849A1 (en) * 2016-05-11 2017-12-28 The University Of North Carolina At Chapel Hill Compositions and methods for bioengineered tissues
KR101895669B1 (ko) * 2016-06-22 2018-09-05 한국과학기술원 접착성 헤파린과 콜라겐을 이용한 줄기세포의 배양방법
GB2569463A (en) * 2016-07-18 2019-06-19 Harvard College Human lymphoid tissue-on-chip
JP7165326B2 (ja) * 2016-12-28 2022-11-04 株式会社高研 高強度コラーゲンスポンジ
WO2025244838A1 (en) * 2024-05-21 2025-11-27 Corning Incorporated Improved collagen coatings

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6069005A (en) * 1991-08-07 2000-05-30 Albert Einstein College Of Medicine Of Yeshwa University Hapatoblasts and method of isolating same
AU667680B2 (en) * 1991-08-07 1996-04-04 Albert Einstein College Of Medicine Of Yeshiva University Proliferation of hepatocyte precursors
DE4222345A1 (de) * 1992-07-08 1994-01-13 Augustinus Dr Med Bader Verfahren zum Züchten einer Zellart im Kokulturverfahren mit Leberzellen
HK1046930B (zh) * 1999-01-19 2012-08-10 查珀尔希尔北卡罗来纳大学 人肝脏祖先
US20020187133A1 (en) * 1999-10-01 2002-12-12 Hiroshi Kubota Methods of isolating bipotent hepatic progenitor cells
US7456017B2 (en) * 1999-10-01 2008-11-25 University Of North Carolina At Chapel Hill Processes for clonal growth of hepatic progenitor cells
PL372619A1 (en) * 2002-03-15 2005-07-25 University Of North Carolina At Chapel Hill Primitive and proximal hepatic stem cells
CA2680254A1 (en) * 2007-03-06 2008-09-12 University Of North Carolina At Chapel Hill Complexes of hyaluronans, other matrix components, hormones and growth factors for maintenance, expansion and/or differentiation of hepatic cells

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102985130A (zh) * 2010-05-07 2013-03-20 北卡罗来纳大学查伯山分校 移植来自实体组织的细胞的方法
CN111281890A (zh) * 2010-05-07 2020-06-16 北卡罗来纳大学查伯山分校 移植来自实体组织的细胞的方法
CN105189738A (zh) * 2013-04-08 2015-12-23 国立研究开发法人医药基盘·健康·营养研究所 肝母细胞样细胞的培养方法及其培养物
CN108431209A (zh) * 2015-06-12 2018-08-21 新加坡科技研究局 肝脏干细胞的衍生和成熟肝细胞类型及其用途
CN108431209B (zh) * 2015-06-12 2023-02-10 新加坡科技研究局 肝脏干细胞的衍生和成熟肝细胞类型及其用途
US11795436B2 (en) 2015-06-12 2023-10-24 Agency For Science, Technology And Research Derivation of hepatic stem cells and mature liver cell types and uses thereof
CN106434532A (zh) * 2016-12-22 2017-02-22 叶宗耀 一种培养肝细胞的培养基及其制备方法
CN115948331A (zh) * 2022-10-17 2023-04-11 中国人民解放军西部战区总医院 一种从小鼠胆囊中分离获取间充质干细胞的方法

Also Published As

Publication number Publication date
WO2007059501A3 (en) 2007-09-13
CA2630355A1 (en) 2007-05-24
EP1954804A4 (en) 2009-03-04
AU2006315163A1 (en) 2007-05-24
KR20080097985A (ko) 2008-11-06
RU2008124158A (ru) 2010-01-10
EP1954804A2 (en) 2008-08-13
US20100093077A1 (en) 2010-04-15
JP2009515558A (ja) 2009-04-16
WO2007059501A2 (en) 2007-05-24
BRPI0618724A2 (pt) 2011-09-06
US20070155009A1 (en) 2007-07-05
IL191533A0 (en) 2008-12-29
CR10073A (es) 2008-10-10
TW200730627A (en) 2007-08-16

Similar Documents

Publication Publication Date Title
CN101384705A (zh) 用于肝祖细胞扩增或分化的细胞外基质组分
CA2958851C (en) Method for producing adult liver progenitor cells
Nitou et al. Purification of fetal mouse hepatoblasts by magnetic beads coated with monoclonal anti-e-cadherin antibodies and their in vitro culture
Hookway et al. Aggregate formation and suspension culture of human pluripotent stem cells and differentiated progeny
Arnhold et al. Amniotic‐Fluid Stem Cells: Growth Dynamics and Differentiation Potential after a CD‐117‐Based Selection Procedure
US20220135947A1 (en) Methods for culturing mesenchymal stem cells, products thereof, and applications thereof
US20110207175A1 (en) Multi-culture bioreactor system
TWI448554B (zh) 肝星形細胞前驅體及其分離方法
CN1742082A (zh) 原肝干细胞和近肝干细胞
US20070178586A1 (en) Methods and apparatuses for growing cells
CN101018856B (zh) 使用透明质酸的小型肝细胞选择性培养方法和分离方法
Li et al. Human umbilical cord-derived mesenchymal stem cells differentiate into epidermal-like cells using a novel co-culture technique
Fujimoto et al. Microencapsulated feeder cells as a source of soluble factors for expansion of CD34+ hematopoietic stem cells
Aalders et al. Liquid marble technology to create cost-effective 3D cardiospheres as a platform for in vitro drug testing and disease modelling
CN113631034B (zh) 基质组合物
CN110951686A (zh) 一种造血干细胞体外扩增培养体系和方法
US20080026463A1 (en) Matrix and method for isolation of hepatic progenitor cells
CN114292804B (zh) 一种血管化脂肪类器官培养方法
US20100136598A1 (en) Novel mesenchymal progenitor cells derived from human blastocyst-derived stem cells
KR102218549B1 (ko) 인간 타액선 세포의 배양 방법
MX2008006456A (en) Extracellular matrix components for expansion or differentiation of hepatic progenitors
JP2003525602A (ja) 三次元バイオリアクターにおける、機能的な白血病細胞のエキソビボ産生
Ouyang Embryonic stem cell culture in fibrous bed bioreactor
CN119193465A (zh) 一种从同一肝脏组织中同时提取多类细胞及传代培养的方法

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Open date: 20090311