CN101156527B - Asexual breeding method for fibraurea recisa pierre - Google Patents
Asexual breeding method for fibraurea recisa pierre Download PDFInfo
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- CN101156527B CN101156527B CN200710066170XA CN200710066170A CN101156527B CN 101156527 B CN101156527 B CN 101156527B CN 200710066170X A CN200710066170X A CN 200710066170XA CN 200710066170 A CN200710066170 A CN 200710066170A CN 101156527 B CN101156527 B CN 101156527B
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- cuttage
- huangteng
- pierre
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Abstract
The invention provides an asexual propagation cultivating method for rhubarb vine, and relates to a plant breeding method, in particular to a medicinal plant breeding and cultivating method. The invention uses a twig of the rhubarb vine for cuttage and seedling, and the breeding method is as follows: A. the selection of a cuttage bed; B. the tidying up of the cuttage bed; C. the collection of the cuttage tress; D. the disinfection process of the tress; E. the cottage and management; F. the selection and the tidying up of the planting filed and the growth environment; G. the transplant and the management of the water and the fertilizer; I. to the arranging the rattan for climbing the frame; J. the prevention and cure of the pest. The actualization of the invention can improve the artificial breeding rate of the rhubarb vine, shorten the breeding period, avoid the excess pick of the wild rhubarb vine, protect the resource of the rhubarb vine, realize the industrialization planting of the rhubarb vine, facilitate the development and utilization of the rhubarb vine, provide process material to the pharmacy corporation for bright-marketing product, protect the wild resource, and preserve the water and oil.
Description
Technical field
The present invention relates to the mating system of a plant species, particularly a kind of breeding cultivation method of medicinal plant.
Background technology
The medicinal plant huangteng is the name herba fibraureae recisae also, is menispermaceae, and herba aristolochiae platymiscium herba fibraureae recisae (Fibraurea recisaPierre) climbs up by holding on to the tall and big liane of shape for perennial.Its rhizome is used as medicine.Serial huangteng patent medicine such as the active ingredient that extracts in the huangteng plant--monomer formulation that the hydrochloric acid fibrauretine is made, fibrauretine sheet, fibrauretine parenteral solution, through clinical practice for many years, have broad-spectrum antiseptic, antiviral and reinforcement body defence function strengthens the leukocytes phagocytic ability.To respiratory tract, urinary system infection contamination, gynecological infection multiple pharmacological action evident in efficacy.But wild huangteng is owing to excessively excavate, and resource is withered day by day exhausted, endangered, and the artificial breeding survival rate of huangteng is low, and the cycle is long, is restricting the development and use of huangteng.
Summary of the invention
Purpose of the present invention aims to provide a kind of survival rate height, and breeding cycle is than the pierre cultivation method of short huangteng.
The pierre cultivation method of huangteng of the present invention is promptly used the huangteng epicormic branch cutting, and its mating system is as follows:
A. the selection of cutting bed
Temperature, humidity are controlled at 15-30 ℃ and 80-90% respectively, set up cutting bed;
B. the arrangement of cutting bed
Enclose furrow, put into cutting medium, sterilization in the furrow;
C. the collection of cuttage branch
Select the spray of anosis worm, stalwartness, be cut into the cuttage bar of band leaf, cut off the 1/2-2/3 of blade;
D. branch is disinfected
The sterilization of cuttage bar is soaked with root-growing agent;
E. cuttage and management
The cuttage bar that soaked root-growing agent is inserted in the furrow, treat that root grows after, the nutritious bag of packing into, the training bud, bud is transplanted after growing to 20-30cm;
F. plant the selection and the arrangement of planting plot and growing environment
Select the sandy loam plot in 300-1000 rice above sea level river valley, beat the pool, apply fertilizer to the subsoil before the transplanting;
G. transplant and water and fertilizer management
5-9 transplants rainy season in month, the reaching 1 year then and answer weeding 3-5 time of transplanting, and later annual weeding 1-2 time was topdressed 1-2 time in 1 year;
I. managing rattan puts on the shelf
Managed rattan with tiny branch and climb the tree or put on the shelf in 1 year that transplants;
J. diseases and pests controlling
With stomach toxicity and systemic insecticide insect pests.
The described furrow that enclose of step b are that furrow are surrounded long 20-25cm, and are smooth in the wide 1.2m, furrow.
Described cutting medium is a kind of in river sand, the immature soil, the perlite, and placement thickness is 20-25cm, before cuttage 4-6 days with 0.1% carbendazim water liquid sterilization.
The described selection cuttage of step c bar is the spray of 3-6 month no damage by disease and insect of growth, robust growth, is cut into every at least with the cuttage bar of 1 blade, the joint of going up of petiole stays 1-1.5cm, and petiole joint down stays 5-8cm, is cut into the inclined-plane, and cut off blade 2/3 with scissors, stay the 1/3 cuttage bar that forms the band leaf.
The described sterilization of steps d is 0.1% potassium permanganate solution, and soak time is 1-2 minute, and root-growing agent is a kind of in methyl or the heteroauxin, and its concentration is 600-800ppm, soaks cuttage bar base portion 1-3 minute.
Step e cuttage is that band leaf branch bar is inserted on the matrix cuttage degree of depth 3-5cm.
Cover bed surface with the protruding canopy of film after the cuttage, growing new root through 30-50 days, root grows to the 5-10cm fashionable dress and goes into nutritious bag and cultivate bud after 65-80 days.
The described readjustment of field plot of step f is meant removes weeds, built.
The described base fertilizer of step f, its applied amount are that the 0.3-0.7kg farmyard manure is executed on every pool.
Step g is described topdresses, and is to be farmyard manure, and farmyard manure 1-2kg is executed in every strain at every turn.
The asexual reproduction method of the pierre cultivation method of huangteng of the present invention, for just with temperature, the control of humidity, can be chosen in plastic tunnel or greenhouse etc. can regulate and control in the facility of temp. and humidity to set up cutting bed substantially, in canopy, enclose furrow with brick, can well control the size of furrow, with be convenient to smooth in the furrow, river sand after the employing sterilization is as cutting medium, help suppressing the damage by disease and insect of cuttage bar, improve survival rate, the band leaf branch bar of handling well is inserted in to sterilize waters above the permeable casting bed matrix, can in time cover bed surface after the cuttage with the protruding canopy of film, with the temperature that keeps being suitable for growing, the maintenance of humidity and cutting bed moisture, grow when treating that root grows to 5-10cm behind the new root, the nutritious bag of just can packing into is cultivated bud, treat height of seedling 20-30cm after, remove the covering facility, practice seedling and also can transplant the land for growing field crops after one week.The low-heat river valley of 300-1000 rice above sea level is selected in the land for growing field crops of transplanting, hot and humid climatope, the fertile deep sandy loam plot of soil layer, remove weeds, suitably stay a part of assorted tree, perhaps artificial built is so that huangteng climbs up by holding on to, according to the habit of growth of huangteng, transplanting time preferably is selected in 5-9 rainy season in month, every mu of plantation 1500-2000 strain, every pool is executed the 0.5kg farmyard manure and is made base fertilizer before transplanting, and helps the growth of huangteng, tear nutritious bag when planting off, cover fine earth and water permeablely, to prick soil not dark because of the root system of huangteng, about 5-30cm, mainly cut weeds during weeding with cutter, also available hoe is shallow except that native face weeds, and rich water quality management is mainly looked the growing way situation of huangteng, can topdress 1-2 time in general 1 year, based on farmyard manure, every strain is executed farmyard manure 1-2kg at every turn and is got final product.The rheum officinale Calamus is climbed up by holding on to liane, because of herba fibraureae recisae does not have tendril, the full winding by self climbed the tree, so should strengthen management in 1 year that transplants, allow it progressively climb the tree or to put on the shelf with tiny branch reason rattan, the rheum officinale liana belongs to wild plant, and resistance is stronger, but establishing in large scale has the harm of moth class larva and snail, available stomach toxicity and systemic insecticide as: systemic insecticides such as massacre spray the blade face and prevent and treat.Enforcement of the present invention can improve the artificial breeding rate of huangteng, and has shortened breeding cycle, can avoid the excessive harvesting of wild huangteng effectively, has protected the huangteng resource, and helps the development and use of huangteng.Transplant by above cultivation, realized the industrialization plantation of huangteng, process raw material for pharmacy corporation provides, product has good market, and can protect wild resource, conserves water and soil.
Embodiment
The present invention is described further below in conjunction with accompanying drawing, but be not limited to embodiment.
Embodiment 1
A. the selection of cutting bed
Temperature, humidity are controlled at 25 ℃ and 85% respectively, set up cutting bed;
B. the arrangement of cutting bed
Enclose furrow, furrow surround long 20-25cm, and wide 1.2m size is smooth in the furrow, puts into cutting medium in the furrow, and matrix is a kind of in river sand, the immature soil, the perlite, and placement thickness is 2020-25cm, irrigate sterilization preceding 5 days of cuttage with 0.1% carbendazim water liquid;
C. the collection of cuttage branch
Select the spray of 3-6 month anosis worm of growth, robust growth, be cut into every cuttage bar with 1 blade, the upward joint of petiole stays 1-1.5cm, and petiole joint down stays 5-8cm, is cut into the inclined-plane, and cuts off blade 2/3 with scissors, stays 1/3 to form the cuttage bar of being with leaf;
D. branch is disinfected
Soaked the cuttage bar 1-2 minute with disinfectant, disinfectant is 0.1% potassium permanganate solution, pull control water out after, soaked cuttage bar base portion 1-3 minute with root-growing agent, root-growing agent is a methyl, its concentration is 600ppm;
E. cuttage and management
The cuttage bar that soaked root-growing agent is inserted in the furrow on the matrix, cuttage degree of depth 3-5cm, cover bed surface with the protruding canopy of film after the cuttage, maintain the temperature at 15-30 ℃, humidity remains on 80-90%, and cutting bed moisture remains on about 50%, can grow new root through 30-50 days, when treating that root grows to 5-10cm after 65-80 days, the nutritious bag of just can packing into is cultivated bud.Described nutritious bag is high 17cm, and wide 13cm packs the cuttage branch that sent out roots in the nutritious bag simultaneously with root and nutrition soil, lines up long 20m, and the plurality of rows of wide 1.2m is watered protruding the little protruding canopy that in time builds up about high 1m with film permeable back and covered.Water one time water every 7 days, and spray one time 0.1% carbendazim soup.Also can grow bud in 30-40 days, and remove the covering facility behind 3-6 month height of seedling 20-30cm, practice seedling and also can transplant the land for growing field crops after one week;
F. plant the selection and the arrangement of planting plot and growing environment
Select the low-heat river valley of 300-1000 rice above sea level, hot and humid climatope, the fertile deep sandy loam plot of soil layer, readjustment of field plot is meant removes weeds, suitably stays a part of assorted tree, and perhaps artificial built is so that huangteng climbs up by holding on to;
G. transplant:
5-9 transplants rainy season in month, every mu of plantation of plastic tunnel 1500-2000 strain, every mu of plantation of open-air plantation 200-400 strain, can beat the pool by the specification of 40 * 40 * 30cm, seeding row spacing 50 * 50cm applies fertilizer to the subsoil before the transplanting, and the base fertilizer applied amount is that the 0.5kg farmyard manure is executed on every pool, tear nutritious bag during transplanting off, cover fine earth and water permeable.That transplants reaches 1 year then and answers weeding 3-5 time, and later annual weeding 1-2 time was topdressed 1-2 time in 1 year, topdressed based on farmyard manure, and farmyard manure 1-2kg is executed in every strain at every turn;
I. managing rattan puts on the shelf:
Managed rattan with tiny branch hemp twine and climb the tree or put on the shelf in 1 year that transplants;
J. diseases and pests controlling:
With stomach toxicity and systemic insecticide insect pests;
Embodiment 2
Matrix is the immature soil, and placement thickness is 25cm, and preceding 4 days of cuttage irrigates sterilization with 0.1% carbendazim water liquid, and all the other are with embodiment 1.
Embodiment 3
Matrix is perlite, and placement thickness is 23cm, and preceding 6 days of cuttage irrigates sterilization with 0.1% carbendazim water liquid, and all the other are with embodiment 1.
Embodiment 4
The cuttage bar is selected the spray of 3-6 month anosis worm of growth, robust growth, is cut into every cuttage bar with 1 blade, and the joint of going up of petiole stays 1.5cm, petiole joint down stays 8cm, is cut into the inclined-plane, and cuts off blade 1/2 with scissors, stay 1/2 to form the cuttage bar of being with leaf, all the other are with embodiment 1.
Embodiment 5
Root-growing agent is a heteroauxin, and its concentration is 800ppm, and all the other are with embodiment 1.
Claims (10)
1. the pierre cultivation method of a huangteng is promptly used the huangteng epicormic branch cutting, it is characterized in that, described mating system is as follows:
A. the selection of cutting bed
Temperature, humidity are controlled at 15-30 ℃ and 80-90% respectively, set up cutting bed;
B. the arrangement of cutting bed
Enclose furrow, put into cutting medium, sterilization in the furrow;
C. the collection of cuttage branch
Select the spray of anosis worm, stalwartness, be cut into the cuttage bar of band leaf, cut off the 1/2-2/3 of blade;
D. branch is disinfected
The sterilization of cuttage bar is soaked with root-growing agent;
E. cuttage and management
The cuttage bar that soaked root-growing agent is inserted in the furrow, treat that root grows 5-10cm after, the nutritious bag of packing into, the training bud, bud is transplanted after growing to 20-30cm;
F. plant the selection and the arrangement of planting plot and growing environment
Select the sandy loam plot in 300-1000 rice above sea level river valley, beat the pool, apply fertilizer to the subsoil before the transplanting;
G. transplant and water and fertilizer management
5-9 transplants rainy season in month, transplanting reach weeding in 1 year 3-5 time then, later annual weeding 1-2 time was topdressed 1-2 time in 1 year;
I. managing rattan puts on the shelf
Managed rattan with tiny branch and climb the tree or put on the shelf in 1 year that transplants;
J. diseases and pests controlling
Use the systemic insecticide insect pests.
2. the pierre cultivation method of a kind of huangteng according to claim 1 is characterized in that, the described furrow that enclose of step b are that furrow are surrounded long 20-25cm, and is smooth in the wide 1.2m, furrow.
3. the pierre cultivation method of a kind of huangteng according to claim 1, it is characterized in that, described cutting medium is a kind of in river sand, the immature soil, the perlite, and placement thickness is 20-25cm, before cuttage 4-6 days with 0.1% carbendazim water liquid sterilization.
4. the pierre cultivation method of a kind of huangteng according to claim 1, it is characterized in that, the described selection cuttage of step c bar, be the spray of 3-6 month no damage by disease and insect of growth, robust growth, be cut into every at least with the cuttage bar of 1 blade, the joint of going up of petiole stays 1-1.5cm, petiole joint down stays 5-8cm, be cut into the inclined-plane, and cut off blade 2/3, stay 1/3 to form the cuttage bar of being with leaf with scissors.
5. the pierre cultivation method of a kind of huangteng according to claim 1, it is characterized in that, the described sterilization of steps d is 0.1% potassium permanganate solution, soak time is 1-2 minute, root-growing agent is a kind of in methyl or the heteroauxin, its concentration is 600-800ppm, soaks cuttage bar base portion 1-3 minute.
6. the pierre cultivation method of a kind of huangteng according to claim 1 is characterized in that, step e cuttage is that band leaf branch bar is inserted on the matrix cuttage degree of depth 3-5cm.
7. the pierre cultivation method of a kind of huangteng according to claim 1 is characterized in that, covers bed surface with the protruding canopy of film after the cuttage, growing new root through 30-50 days, and root grows to the 5-10cm fashionable dress and goes into nutritious bag and cultivate bud after 65-80 days.
8. the pierre cultivation method of a kind of huangteng according to claim 1 is characterized in that, the described readjustment of field plot of step f is meant removes weeds, built.
9. the pierre cultivation method of a kind of huangteng according to claim 1 is characterized in that, the described base fertilizer of step f, and its applied amount is that the 0.3-0.7kg farmyard manure is executed on every pool.
10. the pierre cultivation method of a kind of huangteng according to claim 1 is characterized in that, step g is described topdresses, and is to be farmyard manure, and farmyard manure 1-2kg is executed in every strain at every turn.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710066170XA CN101156527B (en) | 2007-09-07 | 2007-09-07 | Asexual breeding method for fibraurea recisa pierre |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710066170XA CN101156527B (en) | 2007-09-07 | 2007-09-07 | Asexual breeding method for fibraurea recisa pierre |
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| Publication Number | Publication Date |
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| CN101156527A CN101156527A (en) | 2008-04-09 |
| CN101156527B true CN101156527B (en) | 2011-05-11 |
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| CN200710066170XA Expired - Fee Related CN101156527B (en) | 2007-09-07 | 2007-09-07 | Asexual breeding method for fibraurea recisa pierre |
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Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101766086B (en) * | 2009-01-04 | 2012-09-12 | 王兴祥 | Method for rapidly cutting and reproducing euphorbia pekinensis |
| CN102177794B (en) * | 2011-01-28 | 2012-06-20 | 云南大围山生物制药有限公司 | Double-row Y-shaped efficient cultivation method for herba fibraureae recisae |
| CN102172139A (en) * | 2011-01-28 | 2011-09-07 | 云南大围山生物制药有限公司 | Cutting and seedling growing method of common fibraurea stem |
| CN102172143B (en) * | 2011-01-28 | 2012-10-03 | 云南大围山生物制药有限公司 | Method for efficiently cultivating N-shaped fibraurea recisa pierre |
| CN102675820A (en) * | 2012-04-27 | 2012-09-19 | 常熟市佳盛农业科技发展有限公司 | Phenolic resin foam medium block for cuttage |
| CN103026887A (en) * | 2013-01-05 | 2013-04-10 | 中国科学院西双版纳热带植物园 | Cutting propagation method for fibraurea recisa pierre |
| CN103609415B (en) * | 2013-11-26 | 2016-01-20 | 芜湖欧标农业发展有限公司 | A kind of matrix of cultivating climbing plant and the method using matrix cottage propagation |
| CN105123226B (en) * | 2015-09-06 | 2018-03-13 | 普洱市民族传统医药研究所 | A kind of Caulis Mayteni cuttage breeding method |
| CN108370677A (en) * | 2016-10-31 | 2018-08-07 | 昆明仟玺农业科技有限公司 | The implantation methods of huangteng |
| CN111149705A (en) * | 2020-03-13 | 2020-05-15 | 云南珍逸德农业科技有限公司 | Tissue culture and rapid propagation method for stem segments of rhubarb vines |
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| EP0151471A2 (en) * | 1984-02-01 | 1985-08-14 | International Paper Company | Asexual propagation of pine trees |
| CN1526278A (en) * | 2003-09-23 | 2004-09-08 | �й���ҵ��ѧ�о�Ժ�ȴ���ҵ�о��� | Tissue culture breeding method for clonic fibraurea stem |
| CN1672492A (en) * | 2005-05-10 | 2005-09-28 | 中国科学院西双版纳热带植物园 | Artificial propagation method of wild fibraurea stem |
| CN1675982A (en) * | 2005-05-10 | 2005-10-05 | 中国科学院西双版纳热带植物园 | Method for continuously utilizing artificial cultivated herba fibraureae recisea strain |
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2007
- 2007-09-07 CN CN200710066170XA patent/CN101156527B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0151471A2 (en) * | 1984-02-01 | 1985-08-14 | International Paper Company | Asexual propagation of pine trees |
| US4550528A (en) * | 1984-02-01 | 1985-11-05 | International Paper Company | Asexual propagation of pine trees |
| CN1526278A (en) * | 2003-09-23 | 2004-09-08 | �й���ҵ��ѧ�о�Ժ�ȴ���ҵ�о��� | Tissue culture breeding method for clonic fibraurea stem |
| CN1672492A (en) * | 2005-05-10 | 2005-09-28 | 中国科学院西双版纳热带植物园 | Artificial propagation method of wild fibraurea stem |
| CN1675982A (en) * | 2005-05-10 | 2005-10-05 | 中国科学院西双版纳热带植物园 | Method for continuously utilizing artificial cultivated herba fibraureae recisea strain |
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