CN101139562A - 一种链霉菌发酵三七皂苷制备稀有人参皂苷Compound K的工艺 - Google Patents
一种链霉菌发酵三七皂苷制备稀有人参皂苷Compound K的工艺 Download PDFInfo
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
菌株 | 底物 | 转化率% | 参考文献 |
Human fecal microfloraBacteroides JY-6Bifidobacterium K-506Eubacterium A-44Fusobacterium K-60 | Rc | 65.210.810.45.31.2 | Bae EA et al:Biol.Pharm.Bull.,2002,25(6),743-747 |
Sphingomonas echinoids GP50 | Rb1 | - | Kim MK et al:J.Microbiol.,2005,43(5),456-462 |
Aspergillus niger | 三七叶总皂苷 | 12.0 | 周佩等:一种制备人参皂苷Compound-K的方法。中国专利申请号:200410018000.0。公开号:CN 1570133A。 |
Aspergillus nigerAbsidic corymbiferaYeast A8 | 人参总皂苷西洋参总皂苷三七叶总皂苷 | 8.06.8- | 韩颖等:中药研究与信息,2005,7(2),17-19 |
Aspergillus nigerr var.Tiegh | Rb1Rd | 59.476.1 | 杨凌等:一种黑曲霉及用其酵解人参皂苷制备稀有低极性人参皂苷的方法。中国专利号:CN02132403.4。 |
酶 | 底物 | 转化率% | 参考文献 |
从Bifidobacterium sp.Int57提取的粗酶从Bifidobacterium sp.SJ32提取的粗酶从Aspergillus niger KCTC 6906提取的粗酶从Aspergillus usamii var.shirousamii KCTC 6956提取的粗酶 | Rb1 | 96.793.995.655.2 | Chi H et al:Biotechnol.Lett.,2005,27(11),765-771 |
从Bifidobacterium sp.Int57提取的粗酶从Bifidobacterium sp.SJ32提取的粗酶从Aspergillus niger KCTC 6906提取的粗酶从Aspergillus usamii var.shirousamii KCTC 6956提取的粗酶 | Rb2 | 92.587.39.710.4 | Chi H et al:Biol Pharm.Bull.,2005,28(11),2102-2105 |
从Bifidobacterium sp. Int57提取的粗酶从Bifidobacterium sp.SJ32提取的粗酶从Aspergillus niger KCTC 6906提取的粗酶从Aspergillus usamii var.shirousamii KCTC 6956提取的粗酶 | Rc | 77.060.99.752.0 | |
从Rhizopus nigricans提取的粗酶从Trichonderma viride提取的粗酶 | 人参总皂苷 | -- | 杨凌等:一种黑曲霉及用其酵解人参皂苷制备稀有低极性人参皂苷的方法。中国专利号:CN02132403.4。 |
β-葡聚糖苷酶 | 三七叶总皂苷 | - | 姜彬慧等:中草药,2004,35(9),986-988。 |
No | δC(mult.)(125MHz) | δH(int.,mult.,JHH Hz)(500MHz) | No | δC(mult.)(125MHz) | δH(int.,mult.,JHH Hz)(500MHz) |
123456789101112131415161718 | 39.6(t)28.4(t)78.4(d)39.7(s)56.5(d)18.9(t)35.3(t)40.2(s)50.5(d)37.5(s)30.9(t)70.4(d)49.6(d)51.6(s)31.1(t)26.8(t)51.9(d)16.5(q) | 3.41(1H,dd,10.7,5.4)0.79(1H,d,11.5)3.92(1H,ddd)0.92(3H,s) | 192021222324252627282930313233343536 | 16.2(q)83.5(s)22.5(q)36.3(t)23.4(t)126.1(d)131.1(s)25.9(q)17.9(q)28.8(q)16.5(q)17.5(q)98.4(d)75.3(d)79.4(d)71.8(d)78.2(d)63.0(t) | 0.86(3H,s)1.61(3H,s)5.25(1H,t,6.7)1.21(3H,s)1.02(3H,s)0.96(3H,s)5.17(1H,d,7.8) |
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