CA2867665C - Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing - Google Patents

Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing Download PDF

Info

Publication number
CA2867665C
CA2867665C CA2867665A CA2867665A CA2867665C CA 2867665 C CA2867665 C CA 2867665C CA 2867665 A CA2867665 A CA 2867665A CA 2867665 A CA2867665 A CA 2867665A CA 2867665 C CA2867665 C CA 2867665C
Authority
CA
Canada
Prior art keywords
flow cell
read head
nucleic acid
cartridge
acid sequencing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2867665A
Other languages
English (en)
French (fr)
Other versions
CA2867665A1 (en
Inventor
Dale Buermann
John A. Moon
Bryan Crane
Mark Wang
Stanley S. Hong
Jason Harris
Matthew Hage
Mark J. Nibbe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Inc
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Priority to CA3138752A priority Critical patent/CA3138752C/en
Publication of CA2867665A1 publication Critical patent/CA2867665A1/en
Application granted granted Critical
Publication of CA2867665C publication Critical patent/CA2867665C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/52—Containers specially adapted for storing or dispensing a reagent
    • B01L3/527—Containers specially adapted for storing or dispensing a reagent for a plurality of reagents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/05—Flow-through cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment
    • G01N2021/0307—Insert part in cell
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0325—Cells for testing reactions, e.g. containing reagents
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/05—Flow-through cuvettes
    • G01N2021/058—Flat flow cell
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6482—Sample cells, cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/062—LED's

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Clinical Laboratory Science (AREA)
  • Optics & Photonics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Optical Measuring Cells (AREA)
  • Saccharide Compounds (AREA)
  • Catalysts (AREA)
CA2867665A 2012-04-03 2013-02-13 Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing Active CA2867665C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA3138752A CA3138752C (en) 2012-04-03 2013-02-13 Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261619784P 2012-04-03 2012-04-03
US61/619,784 2012-04-03
PCT/US2013/025963 WO2013151622A1 (en) 2012-04-03 2013-02-13 Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA3138752A Division CA3138752C (en) 2012-04-03 2013-02-13 Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing

Publications (2)

Publication Number Publication Date
CA2867665A1 CA2867665A1 (en) 2013-10-10
CA2867665C true CA2867665C (en) 2022-01-04

Family

ID=47891920

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2867665A Active CA2867665C (en) 2012-04-03 2013-02-13 Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing
CA3138752A Active CA3138752C (en) 2012-04-03 2013-02-13 Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA3138752A Active CA3138752C (en) 2012-04-03 2013-02-13 Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing

Country Status (13)

Country Link
US (5) US9193996B2 (OSRAM)
EP (2) EP2834622B1 (OSRAM)
JP (1) JP6159391B2 (OSRAM)
KR (3) KR102118211B1 (OSRAM)
CN (1) CN204832037U (OSRAM)
AU (4) AU2013243998B2 (OSRAM)
BR (2) BR122021002358B8 (OSRAM)
CA (2) CA2867665C (OSRAM)
ES (1) ES2949570T3 (OSRAM)
IL (3) IL234864B (OSRAM)
IN (1) IN2014DN07992A (OSRAM)
MX (1) MX337140B (OSRAM)
WO (1) WO2013151622A1 (OSRAM)

Families Citing this family (329)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
RS54482B1 (sr) 2010-04-05 2016-06-30 Prognosys Biosciences, Inc. Biološka testiranja sa prostornim kodiranjem
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
BR122021002358B8 (pt) * 2012-04-03 2022-03-03 Illumina Inc Sistema fluídico
WO2013158686A1 (en) 2012-04-16 2013-10-24 Biological Dynamics, Inc. Nucleic acid sample preparation
US8932815B2 (en) 2012-04-16 2015-01-13 Biological Dynamics, Inc. Nucleic acid sample preparation
USRE50065E1 (en) 2012-10-17 2024-07-30 10X Genomics Sweden Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
US20140274747A1 (en) 2013-03-15 2014-09-18 Illumina, Inc. Super resolution imaging
US9193998B2 (en) 2013-03-15 2015-11-24 Illumina, Inc. Super resolution imaging
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
CN105849275B (zh) 2013-06-25 2020-03-17 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
AU2014305889B2 (en) * 2013-08-08 2017-06-29 Illumina, Inc. Fluidic system for reagent delivery to a flow cell
JP3206040U (ja) 2013-08-28 2016-09-01 イルミナ インコーポレイテッド 光学アライメントツール
US10540783B2 (en) * 2013-11-01 2020-01-21 Illumina, Inc. Image analysis useful for patterned objects
CA3181696A1 (en) 2013-12-03 2015-06-11 Paul BELITZ Methods and systems for analyzing image data
PL3108009T3 (pl) 2014-02-18 2021-10-11 Illumina, Inc. Sposoby i kompozycje do profilowania dna
WO2015138648A1 (en) * 2014-03-11 2015-09-17 Illumina, Inc. Disposable, integrated microfluidic cartridge and methods of making and using same
US10570447B2 (en) 2014-05-16 2020-02-25 Illumina, Inc. Nucleic acid synthesis techniques
RU2682546C2 (ru) * 2014-05-27 2019-03-19 Иллумина, Инк. Системы и способы биохимического анализа, включающие основной прибор и съемный картридж
CN116328860A (zh) * 2014-06-05 2023-06-27 伊鲁米那股份有限公司 用于样品制备或样品分析中的至少一个的包括旋转阀的系统和方法
US20150353989A1 (en) 2014-06-09 2015-12-10 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
WO2016040602A1 (en) 2014-09-11 2016-03-17 Epicentre Technologies Corporation Reduced representation bisulfite sequencing using uracil n-glycosylase (ung) and endonuclease iv
GB201416422D0 (en) * 2014-09-17 2014-10-29 Illumina Cambridge Ltd Flexible tape-based chemistry apparatus
KR20170066540A (ko) 2014-10-09 2017-06-14 일루미나, 인코포레이티드 액체 중 적어도 하나를 효과적으로 격리시키기 위해 비혼화성 액체를 분리하기 위한 방법 및 디바이스
US9897791B2 (en) 2014-10-16 2018-02-20 Illumina, Inc. Optical scanning systems for in situ genetic analysis
DK3215616T3 (da) 2014-11-05 2020-03-02 Illumina Cambridge Ltd Reducering af DNA-skade under prøvepræparering og sekventering ved anvendelse af siderophor-chelatorer
GB201419731D0 (en) 2014-11-05 2014-12-17 Illumina Cambridge Ltd Sequencing from multiple primers to increase data rate and density
EP3218511B1 (en) 2014-11-11 2020-04-22 Illumina Cambridge Limited Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid
WO2016130964A1 (en) 2015-02-13 2016-08-18 Abbott Laboratories Decapping and capping apparatus, systems and methods for use in diagnostic analyzers
USD766761S1 (en) * 2015-03-20 2016-09-20 Life Technologies Corporation Analytical equipment
EP3271073B1 (en) 2015-03-20 2019-06-12 Illumina, Inc. Fluidics cartridge for use in the vertical or substantially vertical position
DK3274692T3 (da) 2015-03-24 2022-08-29 Illumina Inc Fremgangsmåder til billeddannelse af prøver til biologisk eller kemisk analyse
ES2984193T3 (es) 2015-03-31 2024-10-29 Illumina Cambridge Ltd Concatemerización de moldes en la superficie
US11971354B2 (en) 2015-04-08 2024-04-30 Molecular Devices, Llc Methods and systems for fluorescence detection using infrared dyes
US10379046B2 (en) * 2015-04-08 2019-08-13 Molecular Devices, Llc Method and system for multiplexed time-resolved fluorescence detection
HK1250245A1 (zh) 2015-04-10 2018-12-07 Spatial Transcriptomics Ab 生物样本的空间区别、多重核酸分析
US9222935B1 (en) * 2015-05-28 2015-12-29 Pixcell Medical Technologies Ltd Fluid sample analysis system
EP3303584B1 (en) 2015-05-29 2019-10-09 Epicentre Technologies Corporation Methods of analyzing nucleic acids
US10590464B2 (en) 2015-05-29 2020-03-17 Illumina Cambridge Limited Enhanced utilization of surface primers in clusters
CN115261468A (zh) 2015-07-06 2022-11-01 伊卢米纳剑桥有限公司 用于核酸扩增的样品制备
ES2888626T3 (es) 2015-07-27 2022-01-05 Illumina Inc Cartografiado espacial de información de secuencia de ácidos nucleicos
WO2017030999A1 (en) 2015-08-14 2017-02-23 Illumina, Inc. Systems and methods using magnetically-responsive sensors for determining a genetic characteristic
WO2017034868A1 (en) 2015-08-24 2017-03-02 Illumina, Inc. In-line pressure accumulator and flow-control system for biological or chemical assays
JP6720526B2 (ja) * 2015-12-25 2020-07-08 東洋紡株式会社 検査装置
CA3008031A1 (en) * 2016-01-11 2017-07-20 Illumina Singapore Pte Ltd Detection apparatus having a microfluorometer, a fluidic system, and a flow cell latch clamp module
USD819829S1 (en) * 2016-02-12 2018-06-05 Illumina, Inc. Sequencing cartridge
US10508305B2 (en) * 2016-02-28 2019-12-17 Damoun Nashtaali DNA sequencing and processing
CN109154599A (zh) 2016-03-24 2019-01-04 生物动力学公司 一次性射流卡盘及组件
PL3377226T3 (pl) 2016-03-28 2021-07-26 Illumina, Inc. Wielopłaszczyznowe mikromacierze
ITUA20162851A1 (it) * 2016-04-04 2017-10-04 Euritmi S A S Di Dalla Sega Daniela E C Sistema per microscopia
ES2873723T3 (es) 2016-07-22 2021-11-03 Univ Oregon Health & Science Colecciones de genoma completo de células individuales y métodos de indexación combinatoria para prepararlas
US11547996B2 (en) 2016-08-05 2023-01-10 Vital Signs Solutions Limited Device and method for liquid analysis to detect biomarkers
EP3516082A4 (en) * 2016-09-23 2020-07-01 Archerdx, Inc. NUCLEIC ACID PREPARATION SYSTEM
WO2018064116A1 (en) 2016-09-28 2018-04-05 Illumina, Inc. Methods and systems for data compression
MY194951A (en) 2016-10-14 2022-12-28 Illumina Inc Cartridge assembly
USD825078S1 (en) * 2016-10-14 2018-08-07 Illumina, Inc. Sequencing cartridge
CN108265112B (zh) * 2016-12-30 2023-04-25 深圳市真迈生物科技有限公司 对序列测定反应进行控制的方法、序列测定系统和控制装置
GB201704761D0 (en) * 2017-01-05 2017-05-10 Illumina Inc Common line selector valve for a system
GB201704766D0 (en) * 2017-01-05 2017-05-10 Illumia Inc System and methods for selective effluent collection
GB201704747D0 (en) 2017-01-05 2017-05-10 Illumina Inc Reagent mixing system and methods
GB201704763D0 (OSRAM) * 2017-01-05 2017-05-10 Illumina Inc
GB201704772D0 (en) * 2017-01-05 2017-05-10 Illumina Inc Automated volumetric reagent delivery testing
GB201704768D0 (en) * 2017-01-05 2017-05-10 Illumina Inc Flow cell liquid degassing systema and method
GB201704760D0 (en) 2017-01-05 2017-05-10 Illumina Inc Reagent nozzle sipper mixing system and method
LT3566158T (lt) 2017-01-06 2022-06-27 Illumina, Inc. Fazinė korekcija
TWI689720B (zh) * 2017-01-07 2020-04-01 美商伊路米納有限公司 固態檢驗設備及使用方法
WO2018136416A1 (en) 2017-01-17 2018-07-26 Illumina, Inc. Oncogenic splice variant determination
GB201701689D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials of non-closed shapes
GB201701688D0 (en) * 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials in non-recliner layouts
WO2018144531A1 (en) 2017-02-01 2018-08-09 Illumina, Inc. System and method with fiducials responding to multiple excitation frequencies
GB201701691D0 (en) 2017-02-01 2017-03-15 Illumina Inc System and method with reflective fiducials
GB201701686D0 (en) 2017-02-01 2017-03-15 Illunina Inc System & method with fiducials having offset layouts
KR102373741B1 (ko) * 2017-03-07 2022-03-11 일루미나, 인코포레이티드 단일 광원, 2-광학 채널 서열분석
EP3601597A4 (en) * 2017-03-22 2020-12-23 The Board of Trustees of the University of Illinois SYSTEM FOR RAPID, PORTABLE AND MULTIPLEXED DETECTION AND IDENTIFICATION OF SPECIFIC NUCLEIC ACID SEQUENCES OF PATHOGENIC AGENTS
EP4462437A3 (en) 2017-03-30 2025-03-26 Illumina, Inc. Genomic data analysis system and method
EP3607087A4 (en) 2017-04-04 2020-12-30 Omniome, Inc. FLUIDIC APPARATUS AND USEFUL METHODS FOR CHEMICAL AND BIOLOGICAL REACTIONS
EP3615671B1 (en) 2017-04-23 2021-07-21 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
EP3622084A4 (en) 2017-05-08 2021-02-17 Biological Dynamics, Inc. PROCESSES AND SYSTEMS FOR PROCESSING INFORMATION ON ANALYTES
CN110997932B (zh) 2017-06-07 2024-06-04 俄勒冈健康科学大学 用于甲基化测序的单细胞全基因组文库
US11702654B2 (en) 2017-06-20 2023-07-18 Illumina, Inc. Methods and compositions for addressing inefficiencies in amplification reactions
WO2019027767A1 (en) 2017-07-31 2019-02-07 Illumina Inc. SEQUENCING SYSTEM COMPRISING AGGREGATION OF MULTIPLEXED BIOLOGICAL SAMPLES
CN111108219B (zh) 2017-08-01 2024-12-27 伊鲁米纳公司 使用的水凝胶珠和流动池的遗传物质的空间索引和文库制备
JP2021500531A (ja) 2017-08-15 2021-01-07 オムニオム インコーポレイテッドＯｍｎｉｏｍｅ， Ｉｎｃ． 化学的および生物学的検体の検出に有用なスキャン装置および方法
WO2019036055A2 (en) 2017-08-18 2019-02-21 Ignite Biosciences, Inc. METHODS OF SELECTING BINDING REAGENTS
WO2019116294A1 (en) * 2017-12-15 2019-06-20 Creoptix Ltd. Assemblies and methods for screening sample fluids
EP3724636A4 (en) 2017-12-15 2021-08-18 Evanostics, LLC OPTICAL READER FOR ANALYTE CHECK
JP2021509265A (ja) 2017-12-19 2021-03-25 バイオロジカル ダイナミクス，インク． 生体サンプルからの複数の分析物の検出のための方法およびデバイス
EP3735259A4 (en) 2017-12-29 2021-09-08 Nautilus Biotechnology, Inc. DECODING PROTEIN IDENTIFICATION METHOD
KR102383799B1 (ko) 2018-04-02 2022-04-05 일루미나, 인코포레이티드 서열-기반의 유전 검사용 대조군을 제조하기 위한 조성물 및 방법
JP2021520218A (ja) 2018-04-02 2021-08-19 バイオロジカル ダイナミクス，インク． 誘電体材料
US11441978B1 (en) * 2018-04-12 2022-09-13 Elemental Scientific, Inc. Automatic evaporative sample preparation
WO2019203986A1 (en) 2018-04-19 2019-10-24 Omniome, Inc. Improving accuracy of base calls in nucleic acid sequencing methods
NL2021147B1 (en) * 2018-05-15 2019-11-21 Illumina Inc Flow cell with flexible connection
WO2019221913A1 (en) 2018-05-15 2019-11-21 Illumina, Inc. Flow cell with flexible connection
IL271454B2 (en) 2018-05-17 2025-04-01 Illumina Inc Rapid single-cell genetic sequencing with low Aggregation bias
ES2966028T3 (es) 2018-06-04 2024-04-18 Illumina Inc Bibliotecas de transcriptomas unicelulares de alto rendimiento y métodos de preparación y de uso
JP6688342B2 (ja) * 2018-07-06 2020-04-28 日本板硝子株式会社 反応処理装置
DE102018212489A1 (de) 2018-07-26 2020-01-30 Implen GmbH Vorrichtung für eine lichtspektroskopische Analyse
EP4603594A3 (en) 2018-08-28 2025-11-12 10X Genomics, Inc. Increasing spatial array resolution
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
US20210317524A1 (en) 2018-08-28 2021-10-14 10X Genomics, Inc. Resolving spatial arrays
US11426723B2 (en) * 2018-10-05 2022-08-30 Illumina, Inc. System and method for sequestered wash buffered reuse
NL2021970B1 (en) * 2018-10-05 2020-05-12 Illumina Inc System and method for sequestered wash buffered reuse
WO2020082030A1 (en) 2018-10-18 2020-04-23 The Regents Of The University Of California Device and method for motility-based label-free detection of motile objects in a fluid sample
US10704094B1 (en) 2018-11-14 2020-07-07 Element Biosciences, Inc. Multipart reagents having increased avidity for polymerase binding
US10768173B1 (en) * 2019-09-06 2020-09-08 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
WO2020106889A1 (en) 2018-11-20 2020-05-28 Nautilus Biotechnology, Inc. Design and selection of affinity reagents
US10710076B2 (en) 2018-12-04 2020-07-14 Omniome, Inc. Mixed-phase fluids for nucleic acid sequencing and other analytical assays
CN111277744B (zh) * 2018-12-04 2022-04-15 武汉华大智造科技有限公司 对焦方法及系统、基因测序仪、计算机可读存储介质
WO2020123318A1 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Resolving spatial arrays using deconvolution
WO2020123156A1 (en) * 2018-12-11 2020-06-18 Carrier Corporation Calibration of an optical detector using a micro-flow chamber
AU2019400090B2 (en) * 2018-12-14 2025-11-27 Illumina Cambridge Limited Decreasing phasing with unlabeled nucleotides during sequencing
WO2020132146A1 (en) 2018-12-18 2020-06-25 Evanostics Llc Optical analyte detection
CN113227348B (zh) 2018-12-20 2025-05-27 加利福尼亚太平洋生物科学股份有限公司 用于分析核酸和其他分析物的温度控制
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11493441B2 (en) 2019-01-15 2022-11-08 Wyatt Technology Corporation Flow cell, read head, and skid attachment
EP3924513B1 (en) 2019-02-14 2023-04-12 Pacific Biosciences of California, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
US11680950B2 (en) 2019-02-20 2023-06-20 Pacific Biosciences Of California, Inc. Scanning apparatus and methods for detecting chemical and biological analytes
CN114174531A (zh) 2019-02-28 2022-03-11 10X基因组学有限公司 用空间条码化寡核苷酸阵列对生物分析物进行概况分析
WO2020180778A1 (en) 2019-03-01 2020-09-10 Illumina, Inc. High-throughput single-nuclei and single-cell libraries and methods of making and of using
EP3938538A1 (en) 2019-03-15 2022-01-19 10X Genomics, Inc. Methods for using spatial arrays for single cell sequencing
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
NL2023312B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based base calling
NL2023310B1 (en) 2019-03-21 2020-09-28 Illumina Inc Training data generation for artificial intelligence-based sequencing
NL2023316B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based sequencing
WO2020191389A1 (en) 2019-03-21 2020-09-24 Illumina, Inc. Training data generation for artificial intelligence-based sequencing
NL2023314B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based quality scoring
NL2023311B9 (en) 2019-03-21 2021-03-12 Illumina Inc Artificial intelligence-based generation of sequencing metadata
WO2020198071A1 (en) 2019-03-22 2020-10-01 10X Genomics, Inc. Three-dimensional spatial analysis
CA3135206A1 (en) 2019-04-29 2020-11-05 Pierre Indermuhle Methods and systems for integrated on-chip single-molecule detection
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
WO2020252186A1 (en) 2019-06-11 2020-12-17 Omniome, Inc. Calibrated focus sensing
US10656368B1 (en) 2019-07-24 2020-05-19 Omniome, Inc. Method and system for biological imaging using a wide field objective lens
CN114728996B (zh) 2019-09-10 2022-11-29 加利福尼亚太平洋生物科学股份有限公司 核苷酸的可逆修饰
KR20220063116A (ko) 2019-09-18 2022-05-17 일루미나, 인코포레이티드 시스템 및 관련 샘플 로딩 매니폴드 조립체
EP4031284A4 (en) * 2019-09-18 2023-10-18 Illumina Inc SYSTEMS AND ASSOCIATED PUMP DISTRIBUTION ARRANGEMENTS
JP7457798B2 (ja) * 2019-09-24 2024-03-28 深▲セン▼華大智造科技股▲ふん▼有限公司 生化学物質分析システム、方法及び装置
EP4038546B1 (en) 2019-10-01 2024-08-21 10X Genomics, Inc. Systems and methods for identifying morphological patterns in tissue samples
JP2023502329A (ja) 2019-10-29 2023-01-24 クアンタム－エスアイ インコーポレイテッド 流体の蠕動運動式送出、並びに関連する方法、システム、及び機器
CA3159569A1 (en) * 2019-10-29 2021-05-06 Quantum-Si Incorporated Peristaltic pumping of fluids for bioanalytical applications and associated methods, systems, and devices
US12157124B2 (en) 2019-11-06 2024-12-03 10X Genomics, Inc. Imaging system hardware
EP4055185A1 (en) 2019-11-08 2022-09-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
US12154266B2 (en) 2019-11-18 2024-11-26 10X Genomics, Inc. Systems and methods for binary tissue classification
WO2021102039A1 (en) 2019-11-21 2021-05-27 10X Genomics, Inc, Spatial analysis of analytes
DE202019106694U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
DE202019106695U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
KR20220118295A (ko) 2019-12-19 2022-08-25 일루미나, 인코포레이티드 고 처리량 단일 세포 라이브러리, 및 이의 제조 방법 및 사용 방법
KR20220121819A (ko) * 2019-12-30 2022-09-01 일루미나, 인코포레이티드 플로우 셀 조립체 및 관련 시약 선택기 밸브
US11815458B2 (en) * 2019-12-31 2023-11-14 Illumina, Inc. Autofocus functionality in optical sample analysis
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11408032B2 (en) * 2020-01-17 2022-08-09 Element Biosciences, Inc. Tube lens design for improved depth-of-field
JP7233385B2 (ja) * 2020-01-20 2023-03-06 日本板硝子株式会社 反応処理装置
US12112833B2 (en) 2020-02-04 2024-10-08 10X Genomics, Inc. Systems and methods for index hopping filtering
CN115243792A (zh) 2020-02-04 2022-10-25 加利福尼亚太平洋生物科学股份有限公司 流通池及其制造和使用方法
CN115428088A (zh) 2020-02-13 2022-12-02 10X基因组学有限公司 用于基因表达和dna染色质可及性的联合交互式可视化的系统和方法
EP4107284A1 (en) 2020-02-17 2022-12-28 10X Genomics, Inc. In situ analysis of chromatin interaction
US12354008B2 (en) 2020-02-20 2025-07-08 Illumina, Inc. Knowledge distillation and gradient pruning-based compression of artificial intelligence-based base caller
EP4107285B1 (en) 2020-02-21 2024-10-09 10X Genomics, Inc. Methods and compositions for integrated in situ spatial assay
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
EP4150065A2 (en) 2020-05-12 2023-03-22 Illumina Inc Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase
US12416603B2 (en) 2020-05-19 2025-09-16 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252617A1 (en) 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
AU2021288692A1 (en) 2020-06-11 2023-02-02 Nautilus Subsidiary, Inc. Methods and systems for computational decoding of biological, chemical, and physical entities
KR20230037503A (ko) 2020-06-30 2023-03-16 일루미나, 인코포레이티드 무흔적 dna를 생성하기 위한 촉매적으로 제어된 합성에 의한 서열분석
US12168801B1 (en) 2020-07-02 2024-12-17 10X Genomics, Inc. Hybrid/capture probe designs for full-length cDNA
AU2021306281A1 (en) 2020-07-08 2023-01-05 Illumina, Inc. Beads as transposome carriers
ES2993269T3 (en) 2020-09-18 2024-12-26 10X Genomics Inc Sample handling apparatus and image registration methods
CN114682310B (zh) * 2020-12-31 2023-12-05 深圳市真迈生物科技有限公司 液路系统、测序系统和方法
WO2022068681A1 (zh) * 2020-09-29 2022-04-07 深圳市真迈生物科技有限公司 流动路径选择阀、系统、方法、存储介质及应用
CN114688301A (zh) * 2020-12-31 2022-07-01 深圳市真迈生物科技有限公司 流动路径选择阀、流动池组件、液路系统及测序系统
CN114321036A (zh) * 2020-09-29 2022-04-12 深圳市真迈生物科技有限公司 液路系统、检测系统和应用
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
US11692217B2 (en) 2020-11-11 2023-07-04 Nautilus Subsidiary, Inc. Affinity reagents having enhanced binding and detection characteristics
GB2603451B (en) * 2020-11-25 2024-10-02 Oxford Nanoimaging Ltd Reagent cartridge and measurement devices incorporating such cartridges
EP4012046A1 (en) 2020-12-11 2022-06-15 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
US12449414B2 (en) 2020-12-31 2025-10-21 Quiver Holdings Inc. Calibrating and normalizing an imager for optogenetics
JP2024501850A (ja) 2020-12-31 2024-01-16 キュー－ステート バイオサイエンシーズ， インコーポレイテッド 空間撮像およびビームホモジナイザを伴う顕微鏡
EP4271982A4 (en) * 2020-12-31 2024-11-27 Q-State Biosciences, Inc. Optical plate controller and reader
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
WO2022159663A1 (en) 2021-01-21 2022-07-28 Nautilus Biotechnology, Inc. Systems and methods for biomolecule preparation
WO2022164809A1 (en) 2021-01-26 2022-08-04 10X Genomics, Inc. Nucleic acid analog probes for in situ analysis
EP4421491A3 (en) 2021-02-19 2024-11-27 10X Genomics, Inc. Method of using a modular assay support device
CN115803116A (zh) * 2021-03-03 2023-03-14 伊鲁米纳公司 试剂盒以及用于控制试剂温度的相关系统和方法
US20220282319A1 (en) 2021-03-03 2022-09-08 10X Genomics, Inc. Analyte detection in situ using nucleic acid origami
IL305336B2 (en) 2021-03-11 2025-09-01 Nautilus Subsidiary Inc Systems and methods for preserving biomolecules
CN117043867B (zh) 2021-04-02 2025-11-28 因美纳有限公司 用于检测用于测序的核苷酸样品玻片内的气泡的机器学习模型
US20220380838A1 (en) 2021-06-01 2022-12-01 10X Genomics, Inc. Methods and compositions for analyte detection and probe resolution
EP4347880A1 (en) 2021-06-02 2024-04-10 10X Genomics, Inc. Sample analysis using asymmetric circularizable probes
US20220403450A1 (en) 2021-06-03 2022-12-22 Illumina Software, Inc. Systems and methods for sequencing nucleotides using two optical channels
CN117597453A (zh) 2021-06-23 2024-02-23 伊鲁米纳公司 用于测序试剂的组合物、方法、试剂盒、料筒和系统
CN117651605A (zh) 2021-06-25 2024-03-05 伊鲁米纳公司 在图案化表面的配准中使用的基准
EP4359129A1 (en) 2021-06-25 2024-05-01 Illumina, Inc. Linear fourier fiducial
CN117546245A (zh) 2021-06-29 2024-02-09 因美纳有限公司 用于生成基因组坐标的置信度分类的机器学习模型
CN117730372A (zh) 2021-06-29 2024-03-19 因美纳有限公司 用于确定核苷酸碱基检出和碱基检出质量的信噪比度量
EP4363832A4 (en) 2021-06-30 2025-05-07 Q-State Biosciences, Inc. MULTIPLEX OPTICAL STIMULUS AND READOUT PROCESS
US20230026886A1 (en) 2021-07-13 2023-01-26 10X Genomics, Inc. Methods for preparing polymerized matrix with controllable thickness
US11455487B1 (en) 2021-10-26 2022-09-27 Illumina Software, Inc. Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling
JP2024532004A (ja) 2021-07-19 2024-09-04 イルミナ インコーポレイテッド ベースコールのための補間及び適合による強度抽出
EP4373968A4 (en) 2021-07-21 2025-05-14 Element Biosciences, Inc. Optical systems for nucleic acid sequencing and methods therefor
US20230021577A1 (en) 2021-07-23 2023-01-26 Illumina Software, Inc. Machine-learning model for recalibrating nucleotide-base calls
US12360105B2 (en) 2021-07-30 2025-07-15 10X Genomics, Inc. Methods and compositions for synchronizing reactions in situ
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
US12460251B2 (en) 2021-08-03 2025-11-04 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid molecules
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
US20230057571A1 (en) 2021-08-03 2023-02-23 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
US20230051226A1 (en) * 2021-08-10 2023-02-16 Mgi Tech Co., Ltd. Flow cell imaging systems and methods, and flow cells and other substrates for use in the same
CN117858958A (zh) 2021-08-16 2024-04-09 10X基因组学有限公司 包含分割条形码区的探针和使用方法
WO2023034076A1 (en) 2021-08-31 2023-03-09 Illumina, Inc. Flow cell with enhanced well imaging resolution
KR20240049214A (ko) 2021-09-01 2024-04-16 일루미나, 인코포레이티드 가속 염기 호출을 위한 진폭 변조
CA3227872A1 (en) 2021-09-09 2023-03-16 James Henry JOLY Characterization and localization of protein modifications
USD1064308S1 (en) 2021-09-17 2025-02-25 10X Genomics, Inc. Sample handling device
EP4402682A1 (en) 2021-09-17 2024-07-24 Illumina, Inc. Automatically identifying failure sources in nucleotide sequencing from base-call-error patterns
EP4405954A1 (en) 2021-09-21 2024-07-31 Illumina, Inc. A graph reference genome and base-calling approach using imputed haplotypes
WO2023049073A1 (en) 2021-09-22 2023-03-30 Nautilus Biotechnology, Inc. Methods and systems for determining polypeptide interactions
WO2023049212A2 (en) 2021-09-22 2023-03-30 Illumina, Inc. State-based base calling
AU2022367166A1 (en) 2021-10-11 2024-04-04 Nautilus Subsidiary, Inc. Highly multiplexable analysis of proteins and proteomes
WO2023081728A1 (en) 2021-11-03 2023-05-11 Nautilus Biotechnology, Inc. Systems and methods for surface structuring
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
WO2023102354A1 (en) 2021-12-02 2023-06-08 Illumina Software, Inc. Generating cluster-specific-signal corrections for determining nucleotide-base calls
EP4441711A1 (en) 2021-12-20 2024-10-09 10X Genomics, Inc. Self-test for pathology/histology slide imaging device
WO2023122589A2 (en) * 2021-12-22 2023-06-29 Nautilus Subsidiary, Inc. Systems and methods for carrying out highly multiplexed bioanalyses
WO2023122363A1 (en) 2021-12-23 2023-06-29 Illumina Software, Inc. Dynamic graphical status summaries for nucelotide sequencing
US20230215515A1 (en) 2021-12-23 2023-07-06 Illumina Software, Inc. Facilitating secure execution of external workflows for genomic sequencing diagnostics
US20230242974A1 (en) 2021-12-27 2023-08-03 10X Genomics, Inc. Methods and compositions for rolling circle amplification
US20230207050A1 (en) 2021-12-28 2023-06-29 Illumina Software, Inc. Machine learning model for recalibrating nucleotide base calls corresponding to target variants
KR20240131386A (ko) 2021-12-29 2024-08-30 일루미나, 인코포레이티드 유전체 분석 애플리케이션을 위한 변이 분석 모델 버전 자동 스위칭
CN118475822A (zh) * 2021-12-30 2024-08-09 伊鲁米那有限公司 成像系统以及相关系统和方法
EP4466376A1 (en) 2022-01-21 2024-11-27 10X Genomics, Inc. Multiple readout signals for analyzing a sample
EP4479927A1 (en) 2022-02-17 2024-12-25 Illumina, Inc. Ai-driven signal enhancement of sequencing images
WO2023158809A1 (en) 2022-02-17 2023-08-24 Illumina, Inc. Ai-driven enhancement of motion blurred sequencing images
US20240254537A1 (en) 2022-02-23 2024-08-01 Insitro, Inc. Pooled optical screening and transcriptional measurements of cells comprising barcoded genetic perturbations
EP4483371A1 (en) 2022-02-25 2025-01-01 Illumina, Inc. Machine-learning models for detecting and adjusting values for nucleotide methylation levels
WO2023164660A1 (en) 2022-02-25 2023-08-31 Illumina, Inc. Calibration sequences for nucelotide sequencing
WO2023192917A1 (en) 2022-03-29 2023-10-05 Nautilus Subsidiary, Inc. Integrated arrays for single-analyte processes
EP4505177A1 (en) 2022-04-01 2025-02-12 10x Genomics, Inc. Compositions and methods for targeted masking of autofluorescence
WO2023196526A1 (en) 2022-04-06 2023-10-12 10X Genomics, Inc. Methods for multiplex cell analysis
KR20250004634A (ko) 2022-04-07 2025-01-08 일루미나, 인코포레이티드 변경된 시티딘 데아미나제 및 사용 방법
WO2023212490A1 (en) 2022-04-25 2023-11-02 Nautilus Subsidiary, Inc. Systems and methods for assessing and improving the quality of multiplex molecular assays
EP4515547A1 (en) 2022-04-26 2025-03-05 Illumina, Inc. Machine-learning models for selecting oligonucleotide probes for array technologies
WO2023209606A1 (en) 2022-04-29 2023-11-02 Illumina Cambridge Limited Methods and systems for encapsulating lyophilised microspheres
US20240002902A1 (en) 2022-05-06 2024-01-04 10X Genomics, Inc. Analysis of antigen and antigen receptor interactions
EP4523215A1 (en) 2022-05-10 2025-03-19 Illumina Inc. Adaptive neural network for nucelotide sequencing
JP2025526537A (ja) 2022-06-09 2025-08-15 イルミナ インコーポレイテッド フローセル傾斜に対する塩基コーリングの依存性
EP4540607A1 (en) 2022-06-17 2025-04-23 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
WO2023250364A1 (en) 2022-06-21 2023-12-28 Nautilus Subsidiary, Inc. Method for detecting analytes at sites of optically non-resolvable distances
WO2023250504A1 (en) 2022-06-24 2023-12-28 Illumina Software, Inc. Improving split-read alignment by intelligently identifying and scoring candidate split groups
JP2025523561A (ja) 2022-06-27 2025-07-23 イルミナ インコーポレイテッド 構造多型グラフゲノムの生成及び実装
CA3260497A1 (en) 2022-06-27 2024-01-04 Illumina, Inc. ACCELERATORS FOR A GENOTYPE IMPOTENCY MODEL
WO2024006705A1 (en) 2022-06-27 2024-01-04 Illumina Software, Inc. Improved human leukocyte antigen (hla) genotyping
WO2024026356A1 (en) 2022-07-26 2024-02-01 Illumina, Inc. Rapid single-cell multiomics processing using an executable file
CN119677856A (zh) 2022-08-12 2025-03-21 10X基因组学有限公司 Puma1聚合酶及其用途
CN119677873A (zh) 2022-08-16 2025-03-21 10X基因组学有限公司 Ap50聚合酶及其用途
EP4328569A1 (en) * 2022-08-23 2024-02-28 NanoTemper Technologies GmbH Measuring high protein concentrations
WO2024059655A1 (en) 2022-09-15 2024-03-21 Nautilus Subsidiary, Inc. Characterizing accessibility of macromolecule structures
EP4594012A1 (en) 2022-09-26 2025-08-06 Illumina, Inc. Flow cell based motion system calibration and control methods
EP4595060A1 (en) 2022-09-29 2025-08-06 Illumina, Inc. A target-variant-reference panel for imputing target variants
WO2024073599A1 (en) 2022-09-29 2024-04-04 Nautilus Subsidiary, Inc. Preparation of array surfaces for single-analyte processes
WO2024069581A1 (en) 2022-09-30 2024-04-04 Illumina Singapore Pte. Ltd. Helicase-cytidine deaminase complexes and methods of use
CN118974831A (zh) 2022-09-30 2024-11-15 因美纳有限公司 用于细化结构变体检出的机器学习模型
EP4594343A1 (en) 2022-09-30 2025-08-06 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
WO2024073047A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
CA3260659A1 (en) 2022-10-05 2024-04-11 Illumina, Inc. INTEGRATION OF VARIANT CALLS FROM MULTIPLE SEQUENCE PIPELINES USING A MACHINE LEARNING ARCHITECTURE
EP4602608A1 (en) 2022-10-11 2025-08-20 Illumina, Inc. Detecting and correcting methylation values from methylation sequencing assays
CN120129825A (zh) 2022-10-14 2025-06-10 10X基因组学有限公司 用于评估生物样品质量的方法、组合物和系统
WO2024102736A1 (en) 2022-11-08 2024-05-16 10X Genomics, Inc. Immobilization methods and compositions for in situ detection
WO2024107887A1 (en) 2022-11-16 2024-05-23 10X Genomics, Inc. Methods and compositions for assessing performance of in situ assays
WO2024107727A1 (en) 2022-11-18 2024-05-23 10X Genomics, Inc. Systems and methods for actively mitigating vibrations
EP4627583A1 (en) 2022-11-30 2025-10-08 Illumina, Inc. Accurately predicting variants from methylation sequencing data
AU2022279500B1 (en) * 2022-12-01 2023-11-23 Provectus IP Pty Ltd Automated cell analyser and method
EP4630816A1 (en) 2022-12-09 2025-10-15 Nautilus Subsidiary, Inc. A method comprising performing on a single-analyte array at least 50 cycles of a process
WO2024130000A1 (en) 2022-12-15 2024-06-20 Nautilus Subsidiary, Inc. Inhibition of photon phenomena on single molecule arrays
US20240218437A1 (en) 2022-12-16 2024-07-04 10X Genomics, Inc. Methods and compositions for assessing performance
WO2024137860A1 (en) 2022-12-21 2024-06-27 10X Genomics, Inc. Methods for tethering ribonucleic acids in biological samples
EP4646492A1 (en) 2023-01-06 2025-11-12 10X Genomics, Inc. Methods and compositions for in situ analysis of variant sequences
WO2024151373A1 (en) 2023-01-12 2024-07-18 Nautilus Subsidiary, Inc. Characterization of glycans and glycoconjugates
CN119404215A (zh) 2023-01-27 2025-02-07 因美纳有限公司 抖动校正图像分析
EP4662663A1 (en) 2023-02-06 2025-12-17 Illumina, Inc. Determining and removing inter-cluster light interference
US20240344116A1 (en) 2023-03-24 2024-10-17 Nautilus Subsidiary, Inc. Transfer of nanoparticles to array surfaces
WO2024206848A1 (en) 2023-03-30 2024-10-03 Illumina, Inc. Tandem repeat genotyping
US20240368678A1 (en) 2023-05-03 2024-11-07 10X Genomics, Inc. Methods and compositions for spatial assay
US20240371469A1 (en) 2023-05-03 2024-11-07 Illumina, Inc. Machine learning model for recalibrating genotype calls from existing sequencing data files
US20240394902A1 (en) 2023-05-24 2024-11-28 Nautilus Subsidiary, Inc. Systems and methods for machine vision
WO2024249200A1 (en) 2023-05-26 2024-12-05 Illumina, Inc. Methods for preserving methylation status during clustering
WO2024249940A1 (en) 2023-05-31 2024-12-05 Illumina, Inc. Improving structural variant alignment and variant calling by utilizing a structural-variant reference genome
WO2024249973A2 (en) 2023-06-02 2024-12-05 Illumina, Inc. Linking human genes to clinical phenotypes using graph neural networks
WO2025006565A1 (en) 2023-06-27 2025-01-02 Illumina, Inc. Variant calling with methylation-level estimation
WO2025006874A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Machine-learning model for recalibrating genotype calls corresponding to germline variants and somatic mosaic variants
WO2025006460A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Systems and methods of sequencing polynucleotides with modified bases
WO2025006464A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Systems and methods of sequencing polynucleotides with alternative scatterplots
WO2025006466A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Systems and methods of sequencing polynucleotides with four labeled nucleotides
CN120936702A (zh) * 2023-07-05 2025-11-11 武汉华大智造科技有限公司 用于基因测序的流路系统及其控制方法和基因测序仪
US12319956B2 (en) 2023-07-31 2025-06-03 10X Genomics, Inc. Methods and systems for targeted RNA cleavage and target RNA-primed rolling circle amplification
WO2025038444A1 (en) 2023-08-11 2025-02-20 Illumina, Inc. Optical alignment targets
US20250066841A1 (en) 2023-08-24 2025-02-27 Nautilus Subsidiary, Inc. Compositions and methods for detecting binding interactions under equilibrium or non-equilibrium conditions
WO2025054389A1 (en) 2023-09-07 2025-03-13 Illumina, Inc. Identification of methylated cytosine using landmarks
WO2025050463A1 (en) 2023-09-08 2025-03-13 The Regents Of The University Of Michigan MICRORNA-DERIVED RNAs AND POLYPEPTIDES AND USES THEREOF
WO2025072800A2 (en) 2023-09-28 2025-04-03 Illumina, Inc. Altered cytidine deaminases and methods of use
WO2025072870A1 (en) 2023-09-29 2025-04-03 Illumina, Inc. Tracking and modifying cluster location on nucleotide-sample slides in real time
US20250111899A1 (en) 2023-09-29 2025-04-03 Illumina, Inc. Predicting insert lengths using primary analysis metrics
WO2025081064A2 (en) 2023-10-11 2025-04-17 Illumina, Inc. Thermophilic deaminase and methods for identifying modified cytosine
WO2025090883A1 (en) 2023-10-27 2025-05-01 Illumina, Inc. Detecting variants in nucleotide sequences based on haplotype diversity
WO2025111463A1 (en) 2023-11-22 2025-05-30 10X Genomics, Inc. Methods for processing ribonucleic acids in biological samples
WO2025128588A1 (en) 2023-12-11 2025-06-19 Nautilus Subsidiary, Inc. Methods and compositions of particle-based arrays
WO2025129133A1 (en) 2023-12-15 2025-06-19 Illumina, Inc. Minimal residual disease (mrd) models for determining likelihoods or probabilities of a subject comprising cancer
US20250201346A1 (en) 2023-12-18 2025-06-19 Illumina, Inc. Using machine learning models for detecting minimum residual disease (mrd) in a subject
WO2025137222A1 (en) 2023-12-19 2025-06-26 Illumina, Inc. Methylation detection assay
US20250205706A1 (en) 2023-12-20 2025-06-26 Nautilus Subsidiary, Inc. Fluidic devices for single-analyte assays
WO2025137341A1 (en) 2023-12-20 2025-06-26 Illumina, Inc. Directly determining signal-to-noise-ratio metrics for accelerated convergence in determining nucleotide-base calls and base-call quality
US20250207203A1 (en) 2023-12-20 2025-06-26 10X Genomics, Inc. In situ detection of copy number variations in biological samples
WO2025137647A1 (en) 2023-12-21 2025-06-26 Illumina, Inc. Enhanced mapping and alignment of nucleotide reads utilizing an improved haplotype data structure with allele-variant differences
WO2025160089A1 (en) 2024-01-26 2025-07-31 Illumina, Inc. Custom multigenome reference construction for improved sequencing analysis of genomic samples
WO2025174774A1 (en) 2024-02-12 2025-08-21 Illumina, Inc. Determining offline corrections for sequence specific errors caused by low complexity nucleotide sequences
WO2025174708A1 (en) 2024-02-13 2025-08-21 Illumina, Inc. Design and method for cross-sequencing platform compatibility of flow cells
WO2025184234A1 (en) 2024-02-28 2025-09-04 Illumina, Inc. A personalized haplotype database for improved mapping and alignment of nucleotide reads and improved genotype calling
WO2025193747A1 (en) 2024-03-12 2025-09-18 Illumina, Inc. Machine-learning models for ordering and expediting sequencing tasks or corresponding nucleotide-sample slides
WO2025194033A1 (en) 2024-03-15 2025-09-18 10X Genomics, Inc. Systems and methods for covering and sealing an open well
US20250298016A1 (en) 2024-03-22 2025-09-25 Nautilus Subsidiary, Inc. Plasmonic particle systems for single-analyte assays
WO2025212338A1 (en) 2024-04-01 2025-10-09 Nautilus Subsidiary, Inc. Nanostructures for modulation of analyte conformation
WO2025240241A1 (en) 2024-05-13 2025-11-20 Illumina, Inc. Modifying sequencing cycles during a sequencing run to meet customized coverage estimations for a target genomic region
WO2025240918A1 (en) 2024-05-17 2025-11-20 10X Genomics, Inc. Systems and methods for generating codebooks
WO2025240924A1 (en) 2024-05-17 2025-11-20 Illumina, Inc. Blind equalization systems for base calling applications
WO2025250996A2 (en) 2024-05-31 2025-12-04 Illumina, Inc. Call generation and recalibration models for implementing personalized diploid reference haplotypes in genotype calling
WO2025255457A1 (en) 2024-06-07 2025-12-11 10X Genomics, Inc. Compositions and methods for improved on-target spatial profiling

Family Cites Families (98)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4160263A (en) 1978-05-15 1979-07-03 George R. Cogar Dual or multiple objective video microscope for superimposing spaced images
CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
US6953676B1 (en) * 1992-05-01 2005-10-11 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
DE19536903C2 (de) * 1995-10-04 1998-09-10 Boehringer Ingelheim Int Vorrichtung zum Haltern eines fluidischen Bauteils
US5803117A (en) 1996-06-10 1998-09-08 Rheodyne, L.P. Multi-route full sweep selection valve
US6023540A (en) 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
US20020055100A1 (en) 1997-04-01 2002-05-09 Kawashima Eric H. Method of nucleic acid sequencing
US6399936B1 (en) 1997-12-01 2002-06-04 New Dimension Research Instrument, Inc. Optical confocal device having a common light directing means
US20030036855A1 (en) * 1998-03-16 2003-02-20 Praelux Incorporated, A Corporation Of New Jersey Method and apparatus for screening chemical compounds
US6185030B1 (en) * 1998-03-20 2001-02-06 James W. Overbeck Wide field of view and high speed scanning microscopy
US6012488A (en) 1998-09-17 2000-01-11 Rheodyne, L.P. Segmenting valve
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6326612B1 (en) * 1998-10-13 2001-12-04 Texas Instruments Incorporated System and method for optical sensing utilizing a portable, detachable sensor cartridge
US6424404B1 (en) 1999-01-11 2002-07-23 Kenneth C. Johnson Multi-stage microlens array
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US20030215821A1 (en) 1999-04-20 2003-11-20 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
DK2264189T3 (en) 1999-04-20 2014-12-08 Illumina Inc Detection of nucleic acid reactions of bead - arrays
US6320174B1 (en) 1999-11-16 2001-11-20 Ikonisys Inc. Composing microscope
US20030091477A1 (en) * 1999-12-22 2003-05-15 Paul Eric A. Flow-thru chip cartridge, chip holder, system & method thereof
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
US6526658B1 (en) * 2000-05-23 2003-03-04 Silverbrook Research Pty Ltd Method of manufacture of an ink jet printhead having a moving nozzle with an externally arranged actuator
AU2001282881B2 (en) 2000-07-07 2007-06-14 Visigen Biotechnologies, Inc. Real-time sequence determination
US6603546B1 (en) 2000-07-21 2003-08-05 I.S.S. (Usa) Inc. Rapid high throughput spectrometer and method
US6875973B2 (en) * 2000-08-25 2005-04-05 Amnis Corporation Auto focus for a flow imaging system
US6374684B1 (en) 2000-08-25 2002-04-23 Cepheid Fluid control and processing system
US7514046B2 (en) * 2000-10-31 2009-04-07 Caliper Life Sciences, Inc. Methods and systems for processing microscale devices for reuse
US7378280B2 (en) * 2000-11-16 2008-05-27 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
JP2002219849A (ja) 2000-11-27 2002-08-06 Riso Kagaku Corp 孔版印刷装置
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US7864369B2 (en) 2001-03-19 2011-01-04 Dmetrix, Inc. Large-area imaging by concatenation with array microscope
AU2002307152A1 (en) * 2001-04-06 2002-10-21 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
WO2003025838A1 (en) 2001-09-14 2003-03-27 The Arizona Board Of Regents On Behalf Of The University Of Arizona Inter-objective baffle system
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US20040002090A1 (en) 2002-03-05 2004-01-01 Pascal Mayer Methods for detecting genome-wide sequence variations associated with a phenotype
US6982166B2 (en) 2002-05-16 2006-01-03 Applera Corporation Lens assembly for biological testing
US6987259B2 (en) 2002-05-30 2006-01-17 Dmetrix, Inc. Imaging system with an integrated source and detector array
US7193775B2 (en) 2002-05-30 2007-03-20 Dmetrix, Inc. EPI-illumination system for an array microscope
US7312432B2 (en) 2002-07-08 2007-12-25 Dmetrix, Inc. Single axis illumination for multi-axis imaging system
US7582470B2 (en) * 2002-07-31 2009-09-01 Gen-Probe Incorporated Device for amplifying and detecting a target nucleic acid
JP2006509040A (ja) 2002-08-23 2006-03-16 ソレックサ リミテッド 修飾されたヌクレオチド
US6950241B1 (en) 2002-09-18 2005-09-27 Dmetrix, Inc. Miniature microscope objective for an array microscope
US7388714B2 (en) 2002-09-18 2008-06-17 Dmetrix, Inc. Independent focus compensation for a multi-axis imaging system
US7130115B2 (en) 2002-09-23 2006-10-31 Dhetrix, Inc. Multi-mode scanning imaging system
US7034317B2 (en) 2002-12-17 2006-04-25 Dmetrix, Inc. Method and apparatus for limiting scanning imaging array data to characteristics of interest
US20050221281A1 (en) * 2003-01-08 2005-10-06 Ho Winston Z Self-contained microfluidic biochip and apparatus
EP1594980B2 (en) 2003-01-29 2017-08-16 454 Life Sciences Corporation Bead emulsion nucleic acid amplification
US7330574B2 (en) 2003-05-08 2008-02-12 Ometrix, Inc. Best-focus estimation by lateral scanning
US7148043B2 (en) * 2003-05-08 2006-12-12 Bio-Rad Laboratories, Inc. Systems and methods for fluorescence detection with a movable detection module
US20050181394A1 (en) 2003-06-20 2005-08-18 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
EP2918595B1 (en) 2003-07-05 2019-12-11 The Johns-Hopkins University Method and compositions for detection and enumeration of genetic variations
EP1701785A1 (en) 2004-01-07 2006-09-20 Solexa Ltd. Modified molecular arrays
DE602004004612T2 (de) 2004-05-22 2007-05-24 Agilent Technologies, Inc. (n.d. Ges. d. Staates Delaware), Santa Clara Bauteil eines mikrofluidischen Ventils
WO2006085948A2 (en) * 2004-07-01 2006-08-17 Cornell Research Foundation, Inc. Real-time pcr detection of microorganisms using an integrated microfluidics platform
WO2006018044A1 (en) 2004-08-18 2006-02-23 Agilent Technologies, Inc. Microfluidic assembly with coupled microfluidic devices
US7745207B2 (en) * 2006-02-03 2010-06-29 IntegenX, Inc. Microfluidic devices
US20060062531A1 (en) 2004-09-17 2006-03-23 Stephen Turner Fabrication of optical confinements
CA2587085C (en) * 2004-10-27 2017-08-08 Cepheid Closed-system multi-stage nucleic acid amplification reactions
FI20055043A0 (fi) 2005-01-31 2005-01-31 Wallac Oy Sovitelma mikrotiterkaivojen kuvausjärjestelmässä
US7507575B2 (en) * 2005-04-01 2009-03-24 3M Innovative Properties Company Multiplex fluorescence detection device having removable optical modules
EP1907571B1 (en) 2005-06-15 2017-04-26 Complete Genomics Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
EP1945793A4 (en) * 2005-08-11 2015-11-25 Eksigent Technologies Llc METHOD FOR MEASURING BIOCHEMICAL REACTIONS
JP5058483B2 (ja) * 2005-09-14 2012-10-24 オリンパス株式会社 生体試料の長期間ないし連続的検出方法
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
JP4742242B2 (ja) * 2005-09-30 2011-08-10 カシオ計算機株式会社 撮像装置及びそのプログラム
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
US7329860B2 (en) 2005-11-23 2008-02-12 Illumina, Inc. Confocal imaging methods and apparatus
US20080009420A1 (en) 2006-03-17 2008-01-10 Schroth Gary P Isothermal methods for creating clonal single molecule arrays
EP2018622B1 (en) 2006-03-31 2018-04-25 Illumina, Inc. Systems for sequence by synthesis analysis
US8900828B2 (en) * 2006-05-01 2014-12-02 Cepheid Methods and apparatus for sequential amplification reactions
US8137626B2 (en) * 2006-05-19 2012-03-20 California Institute Of Technology Fluorescence detector, filter device and related methods
US7641860B2 (en) * 2006-06-01 2010-01-05 Nanotek, Llc Modular and reconfigurable multi-stage microreactor cartridge apparatus
US7586674B2 (en) * 2006-10-17 2009-09-08 Hnu-Photonics Compuscope
AU2007309504B2 (en) 2006-10-23 2012-09-13 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US20110001036A1 (en) * 2006-10-24 2011-01-06 Koninklijke Philips Electronics N.V. system for imaging an object
US7813013B2 (en) 2006-11-21 2010-10-12 Illumina, Inc. Hexagonal site line scanning method and system
US9322063B2 (en) 2007-01-26 2016-04-26 Illumina, Inc. Efficient biomolecule recycling method and system
CA2676570C (en) * 2007-01-26 2016-05-03 Illumina, Inc. Nucleic acid sequencing system and method
US8298763B2 (en) * 2007-03-02 2012-10-30 Lawrence Livermore National Security, Llc Automated high-throughput flow-through real-time diagnostic system
US8039817B2 (en) 2008-05-05 2011-10-18 Illumina, Inc. Compensator for multiple surface imaging
DE102008002509A1 (de) * 2008-06-18 2009-12-31 INSTITUT FüR MIKROTECHNIK MAINZ GMBH Stopped-Flow-Chip
WO2010056945A2 (en) * 2008-11-13 2010-05-20 Shalon Ventures, Inc. Methods and systems for tissue processing and imaging
EP2359289A4 (en) 2008-11-26 2015-11-11 Illumina Inc METHODS AND SYSTEMS FOR ANALYZING SEQUENCING DATA
US20100157086A1 (en) * 2008-12-15 2010-06-24 Illumina, Inc Dynamic autofocus method and system for assay imager
US8673627B2 (en) * 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
US8304704B2 (en) * 2009-07-27 2012-11-06 Sensovation Ag Method and apparatus for autofocus using a light source pattern and means for masking the light source pattern
US20110124111A1 (en) * 2009-08-31 2011-05-26 Life Technologies Corporation Low-volume sequencing system and method of use
BR112012013074B1 (pt) 2009-12-01 2018-09-18 Oxford Nanopore Technologies Limited instrumento de análise e módulo para realizar análise bioquímica, e, método para operar um instrumento de análise para realizar análise bioquímica
CA3028780C (en) * 2009-12-07 2022-05-31 Meso Scale Technologies, Llc Assay cartridges and methods of using the same
CA2809581C (en) * 2010-08-27 2016-11-15 The Board Of Trustees Of The Leland Stanford Junior University Microscopy imaging device with advanced imaging properties
CN103370617B (zh) 2010-10-01 2015-11-25 牛津纳米孔技术有限公司 生物化学分析设备和旋转阀
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
BR122021002358B8 (pt) * 2012-04-03 2022-03-03 Illumina Inc Sistema fluídico

Also Published As

Publication number Publication date
US20230137970A1 (en) 2023-05-04
KR20150000885A (ko) 2015-01-05
BR122021002358B1 (pt) 2021-10-26
US20130260372A1 (en) 2013-10-03
IL268205B (en) 2020-03-31
KR20210080618A (ko) 2021-06-30
US20140329694A1 (en) 2014-11-06
IL273034B (en) 2021-12-01
AU2019200400B2 (en) 2020-10-22
KR102118211B1 (ko) 2020-06-02
IL234864A0 (en) 2014-12-31
US10549281B2 (en) 2020-02-04
US20170246635A1 (en) 2017-08-31
JP2015514218A (ja) 2015-05-18
AU2013243998A1 (en) 2014-10-09
MX337140B (es) 2016-02-12
KR102271225B1 (ko) 2021-06-29
CA2867665A1 (en) 2013-10-10
BR112014024789B1 (pt) 2021-05-25
US11565267B2 (en) 2023-01-31
EP2834622A1 (en) 2015-02-11
CN204832037U (zh) 2015-12-02
BR122021002358B8 (pt) 2022-03-03
IL234864B (en) 2019-08-29
AU2016222420B2 (en) 2019-01-03
HK1201582A1 (en) 2015-09-04
US20200139375A1 (en) 2020-05-07
IL268205A (en) 2019-08-29
IN2014DN07992A (OSRAM) 2015-05-01
US9650669B2 (en) 2017-05-16
AU2019200400A1 (en) 2019-02-07
AU2021200408B2 (en) 2023-04-06
EP4219012A1 (en) 2023-08-02
AU2021200408A1 (en) 2021-03-18
MX2014011165A (es) 2015-03-19
AU2013243998B2 (en) 2016-07-28
ES2949570T3 (es) 2023-09-29
EP2834622B1 (en) 2023-04-12
IL273034A (en) 2020-04-30
JP6159391B2 (ja) 2017-07-05
AU2016222420A1 (en) 2016-09-22
KR20200064162A (ko) 2020-06-05
WO2013151622A1 (en) 2013-10-10
CA3138752A1 (en) 2013-10-10
CA3138752C (en) 2024-02-06
US9193996B2 (en) 2015-11-24

Similar Documents

Publication Publication Date Title
CA2867665C (en) Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing
AU2022200683A8 (en) Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
WO2009102688A3 (en) Flow cells and manifolds having an electroosmotic pump
CA2886462C (en) Microfluidic lal-reactive substances testing method and apparatus
MX370560B (es) Sistema de fluidos para distribución de reactivo a una celda de flujo.
CN203899622U (zh) 一种微流控芯片
GB2556703A (en) Systems and methods
CA2931582C (en) Microfluidic cartridge for molecular diagnosis, docking station using such a microfluidic cartridge, and process for analyzing a biological sample
TR201900687T4 (tr) Sivi numune alicisi, parça ki̇ti̇ ve bi̇rleşti̇rmeye yöneli̇k yöntem
WO2013134742A3 (en) Micro-tube particles for microfluidic assays and methods of manufacture
SA518400535B1 (ar) خرطوشة خلية تدفق ذات قوس إحكام طافي
MX2016000506A (es) Metodos y sistemas de transferencia de fluidos con regulacion de presion.
WO2016128843A3 (en) Disposable cartridge for sample fluid analysis
EP2474360A3 (en) Microfluidic device and analyte detection method using the same
CA2867414C (en) A test cartridge with integrated transfer module
EA201500369A1 (ru) Ячеистая трубка
WO2010130762A9 (en) Apparatus for and method of automated processing of biological samples
PH12018500959B1 (en) Apparatus and system for biofluid sample dispensing and/or assay
SG170703A1 (en) Microfluidic system for detecting a biological entity in a sample
JP2013545451A5 (OSRAM)
WO2010075568A3 (en) Biological analysis systems, devices and methods
MX384530B (es) Dispositivos, sistemas, metodos y equipos para recibir un hisopo.
BR112017004191A2 (pt) dispositivo microfluídico, e, método para purificar um analito biológico ou químico.
EP4566715A3 (en) Systems and methods for handling microfluidic droplets
WO2012122458A3 (en) A cartridge based system and method for detecting an analyte in a sample

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20180129