BR112017004191A2 - dispositivo microfluídico, e, método para purificar um analito biológico ou químico. - Google Patents

dispositivo microfluídico, e, método para purificar um analito biológico ou químico.

Info

Publication number
BR112017004191A2
BR112017004191A2 BR112017004191A BR112017004191A BR112017004191A2 BR 112017004191 A2 BR112017004191 A2 BR 112017004191A2 BR 112017004191 A BR112017004191 A BR 112017004191A BR 112017004191 A BR112017004191 A BR 112017004191A BR 112017004191 A2 BR112017004191 A2 BR 112017004191A2
Authority
BR
Brazil
Prior art keywords
chamber
orifice
filter
microfluidic device
flow path
Prior art date
Application number
BR112017004191A
Other languages
English (en)
Other versions
BR112017004191B1 (pt
Inventor
Benitez Porras Francesc
Pareja Gómez Josep
Original Assignee
Stat Diagnostica & Innovation Sl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stat Diagnostica & Innovation Sl filed Critical Stat Diagnostica & Innovation Sl
Publication of BR112017004191A2 publication Critical patent/BR112017004191A2/pt
Publication of BR112017004191B1 publication Critical patent/BR112017004191B1/pt

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0621—Control of the sequence of chambers filled or emptied
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0631—Purification arrangements, e.g. solid phase extraction [SPE]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B01L2200/146—Employing pressure sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0663—Whole sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/14—Means for pressure control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0622—Valves, specific forms thereof distribution valves, valves having multiple inlets and/or outlets, e.g. metering valves, multi-way valves

Abstract

um dispositivo microfluídico é descrito tendo uma câmara encerrada contendo um filtro para purificar analitos biológicos ou químicos a partir de uma amostra biológica complexa, dita câmara alojando uma pluralidade de orifícios em adição ao dito filtro, como se segue: um primeiro orifício permitindo comunicação de gás da câmara com um gerador de vácuo, por um primeiro trajeto de fluxo; um segundo orifício permitindo comunicação de líquido da câmara com um ou mais reservatórios, por um segundo trajeto de fluxo; um terceiro orifício permitindo comunicação de gás e de líquido da câmara com ambos um ou mais recipientes receptores e um gerador de vácuo, por um terceiro trajeto de fluxo; e um filtro localizado entre o terceiro orifício e ambos o primeiro e segundo orifício, para que um fluido entrando na câmara através do primeiro e/ou segundo orifício e saindo da câmara através do terceiro orifício flui através do filtro. a invenção também se refere a um método usando o dispositivo microfluídico.
BR112017004191-0A 2014-09-03 2015-09-02 Dispositivo microfluídico, e, método para purificar um analito biológico ou químico BR112017004191B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP14183411.9A EP2992958A1 (en) 2014-09-03 2014-09-03 Nucleic acid purification cartridge
EP14183411.9 2014-09-03
PCT/EP2015/070045 WO2016034620A1 (en) 2014-09-03 2015-09-02 Nucleic acid purification cartridge

Publications (2)

Publication Number Publication Date
BR112017004191A2 true BR112017004191A2 (pt) 2017-12-12
BR112017004191B1 BR112017004191B1 (pt) 2022-07-05

Family

ID=51518547

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017004191-0A BR112017004191B1 (pt) 2014-09-03 2015-09-02 Dispositivo microfluídico, e, método para purificar um analito biológico ou químico

Country Status (12)

Country Link
US (1) US10793849B2 (pt)
EP (2) EP2992958A1 (pt)
JP (1) JP6800144B2 (pt)
KR (1) KR102458490B1 (pt)
CN (1) CN106999928B (pt)
AU (1) AU2015310942B2 (pt)
BR (1) BR112017004191B1 (pt)
CA (1) CA2959954C (pt)
ES (1) ES2873362T3 (pt)
RU (1) RU2722075C2 (pt)
WO (1) WO2016034620A1 (pt)
ZA (1) ZA201702211B (pt)

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AU2012236099A1 (en) 2011-03-31 2013-10-03 Moderna Therapeutics, Inc. Delivery and formulation of engineered nucleic acids
WO2014152031A1 (en) 2013-03-15 2014-09-25 Moderna Therapeutics, Inc. Ribonucleic acid purification
EP2983804A4 (en) 2013-03-15 2017-03-01 Moderna Therapeutics, Inc. Ion exchange purification of mrna
US10138507B2 (en) 2013-03-15 2018-11-27 Modernatx, Inc. Manufacturing methods for production of RNA transcripts
US10077439B2 (en) 2013-03-15 2018-09-18 Modernatx, Inc. Removal of DNA fragments in mRNA production process
WO2015051169A2 (en) 2013-10-02 2015-04-09 Moderna Therapeutics, Inc. Polynucleotide molecules and uses thereof
US10286086B2 (en) 2014-06-19 2019-05-14 Modernatx, Inc. Alternative nucleic acid molecules and uses thereof
AU2015289656A1 (en) 2014-07-16 2017-02-16 Modernatx, Inc. Circular polynucleotides
WO2017049286A1 (en) 2015-09-17 2017-03-23 Moderna Therapeutics, Inc. Polynucleotides containing a morpholino linker
US20200318098A1 (en) * 2016-06-24 2020-10-08 Modernatx, Inc. Methods and apparatus for filtration
GB201703383D0 (en) 2017-03-02 2017-04-19 Gargle Tech Ltd Testing for particulates
WO2020049569A2 (en) 2018-09-05 2020-03-12 Hero Scientific Ltd. Testing for particulates
KR102065649B1 (ko) * 2017-12-28 2020-01-13 에스디 바이오센서 주식회사 핵산 추출용 카트리지의 피스톤
GB201819417D0 (en) * 2018-11-29 2019-01-16 Quantumdx Group Ltd Vacuum-assisted drying of filters in microfluidic systems
EP4021639A1 (en) * 2019-08-30 2022-07-06 Boehringer Ingelheim Vetmedica GmbH Filter instrument, kit and method for pretreating a sample
CN111545256B (zh) * 2020-03-31 2022-05-13 深圳市刚竹医疗科技有限公司 离心微流控芯片
CA3202405A1 (en) 2021-01-06 2022-07-14 Zvi Feldman Filtration sampling devices
CN112899132B (zh) * 2021-01-13 2021-10-15 上海金鑫生物科技有限公司 一种用于核酸提取与纯化的硅胶过滤吸头

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FR2657543B1 (fr) * 1990-01-26 1992-12-18 Biocom Sa Dispositif modulaire pour le recueil, l'incubation, la filtration d'echantillons multiples.
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Also Published As

Publication number Publication date
AU2015310942B2 (en) 2021-02-04
JP6800144B2 (ja) 2020-12-16
JP2017532016A (ja) 2017-11-02
WO2016034620A1 (en) 2016-03-10
KR102458490B1 (ko) 2022-10-26
ZA201702211B (en) 2018-08-29
EP2992958A1 (en) 2016-03-09
BR112017004191B1 (pt) 2022-07-05
CN106999928B (zh) 2021-05-18
ES2873362T3 (es) 2021-11-03
EP3188838B1 (en) 2021-04-14
KR20170045339A (ko) 2017-04-26
RU2017110274A (ru) 2018-10-03
EP3188838A1 (en) 2017-07-12
RU2017110274A3 (pt) 2019-02-22
CA2959954C (en) 2022-07-19
US20170283792A1 (en) 2017-10-05
AU2015310942A1 (en) 2017-04-20
CA2959954A1 (en) 2016-03-10
CN106999928A (zh) 2017-08-01
US10793849B2 (en) 2020-10-06
RU2722075C2 (ru) 2020-05-26

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Legal Events

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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 02/09/2015, OBSERVADAS AS CONDICOES LEGAIS