CA2742474C - Method for the production of adenoviral vectors - Google Patents
Method for the production of adenoviral vectors Download PDFInfo
- Publication number
- CA2742474C CA2742474C CA2742474A CA2742474A CA2742474C CA 2742474 C CA2742474 C CA 2742474C CA 2742474 A CA2742474 A CA 2742474A CA 2742474 A CA2742474 A CA 2742474A CA 2742474 C CA2742474 C CA 2742474C
- Authority
- CA
- Canada
- Prior art keywords
- cells
- cell
- rad35
- adenovirus
- bioreactor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 title claims abstract description 95
- 238000004519 manufacturing process Methods 0.000 title claims description 55
- 239000013598 vector Substances 0.000 title description 46
- 208000015181 infectious disease Diseases 0.000 claims abstract description 103
- 241000701161 unidentified adenovirus Species 0.000 claims abstract description 97
- 230000010412 perfusion Effects 0.000 claims abstract description 82
- 241000700605 Viruses Species 0.000 claims description 66
- 239000002245 particle Substances 0.000 claims description 48
- 238000003306 harvesting Methods 0.000 claims description 25
- 238000012258 culturing Methods 0.000 claims description 22
- 230000002458 infectious effect Effects 0.000 claims description 10
- 239000000725 suspension Substances 0.000 claims description 10
- 108020004707 nucleic acids Proteins 0.000 claims description 8
- 102000039446 nucleic acids Human genes 0.000 claims description 8
- 150000007523 nucleic acids Chemical class 0.000 claims description 8
- 241000701124 Human adenovirus 35 Species 0.000 claims description 6
- 239000008194 pharmaceutical composition Substances 0.000 claims description 6
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 4
- 210000004027 cell Anatomy 0.000 description 399
- 230000008569 process Effects 0.000 description 42
- 238000004128 high performance liquid chromatography Methods 0.000 description 23
- 239000002609 medium Substances 0.000 description 22
- 238000000746 purification Methods 0.000 description 21
- 238000011534 incubation Methods 0.000 description 20
- 229960005486 vaccine Drugs 0.000 description 20
- 230000000644 propagated effect Effects 0.000 description 17
- 239000001963 growth medium Substances 0.000 description 15
- 238000005571 anion exchange chromatography Methods 0.000 description 14
- 238000004458 analytical method Methods 0.000 description 13
- 241001135569 Human adenovirus 5 Species 0.000 description 12
- 238000005119 centrifugation Methods 0.000 description 12
- 239000012510 hollow fiber Substances 0.000 description 12
- 108010034546 Serratia marcescens nuclease Proteins 0.000 description 11
- 230000002950 deficient Effects 0.000 description 11
- 239000012737 fresh medium Substances 0.000 description 10
- 238000000926 separation method Methods 0.000 description 10
- 101100113692 Caenorhabditis elegans clk-2 gene Proteins 0.000 description 9
- 239000003599 detergent Substances 0.000 description 9
- 238000011161 development Methods 0.000 description 9
- 230000018109 developmental process Effects 0.000 description 9
- 230000035899 viability Effects 0.000 description 9
- 229930006000 Sucrose Natural products 0.000 description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 8
- 229920004890 Triton X-100 Polymers 0.000 description 8
- 239000013504 Triton X-100 Substances 0.000 description 8
- 238000013019 agitation Methods 0.000 description 8
- 238000004113 cell culture Methods 0.000 description 8
- 239000000546 pharmaceutical excipient Substances 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 239000004017 serum-free culture medium Substances 0.000 description 8
- 239000005720 sucrose Substances 0.000 description 8
- 230000008901 benefit Effects 0.000 description 7
- 239000000872 buffer Substances 0.000 description 7
- 239000006285 cell suspension Substances 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- 239000008188 pellet Substances 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 7
- 101710163270 Nuclease Proteins 0.000 description 6
- 238000005349 anion exchange Methods 0.000 description 6
- 230000006037 cell lysis Effects 0.000 description 6
- 230000009089 cytolysis Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 108700039887 Essential Genes Proteins 0.000 description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 5
- 238000010923 batch production Methods 0.000 description 5
- 239000012528 membrane Substances 0.000 description 5
- 229910052760 oxygen Inorganic materials 0.000 description 5
- 239000001301 oxygen Substances 0.000 description 5
- 238000013341 scale-up Methods 0.000 description 5
- 210000004881 tumor cell Anatomy 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 241000282412 Homo Species 0.000 description 4
- 230000006727 cell loss Effects 0.000 description 4
- 238000012217 deletion Methods 0.000 description 4
- 230000037430 deletion Effects 0.000 description 4
- 238000011143 downstream manufacturing Methods 0.000 description 4
- 239000003937 drug carrier Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000007717 exclusion Effects 0.000 description 4
- 238000009472 formulation Methods 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 230000028993 immune response Effects 0.000 description 4
- 238000012809 post-inoculation Methods 0.000 description 4
- 230000036515 potency Effects 0.000 description 4
- 230000002028 premature Effects 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 239000012679 serum free medium Substances 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 108700019146 Transgenes Proteins 0.000 description 3
- 239000002671 adjuvant Substances 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 239000013592 cell lysate Substances 0.000 description 3
- 238000011210 chromatographic step Methods 0.000 description 3
- 238000004587 chromatography analysis Methods 0.000 description 3
- 238000001415 gene therapy Methods 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000006166 lysate Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000002207 metabolite Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 238000005457 optimization Methods 0.000 description 3
- 230000010076 replication Effects 0.000 description 3
- 238000001542 size-exclusion chromatography Methods 0.000 description 3
- 238000004114 suspension culture Methods 0.000 description 3
- 238000000108 ultra-filtration Methods 0.000 description 3
- 230000003612 virological effect Effects 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 2
- 108010074328 Interferon-gamma Proteins 0.000 description 2
- 108700026244 Open Reading Frames Proteins 0.000 description 2
- 238000001190 Q-PCR Methods 0.000 description 2
- 238000004115 adherent culture Methods 0.000 description 2
- 210000004102 animal cell Anatomy 0.000 description 2
- 238000010364 biochemical engineering Methods 0.000 description 2
- 229960000074 biopharmaceutical Drugs 0.000 description 2
- 229940112112 capex Drugs 0.000 description 2
- 239000006143 cell culture medium Substances 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 238000005352 clarification Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000011026 diafiltration Methods 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- FEBLZLNTKCEFIT-VSXGLTOVSA-N fluocinolone acetonide Chemical compound C1([C@@H](F)C2)=CC(=O)C=C[C@]1(C)[C@]1(F)[C@@H]2[C@@H]2C[C@H]3OC(C)(C)O[C@@]3(C(=O)CO)[C@@]2(C)C[C@@H]1O FEBLZLNTKCEFIT-VSXGLTOVSA-N 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 201000004792 malaria Diseases 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000000174 oncolytic effect Effects 0.000 description 2
- 238000013386 optimize process Methods 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- 239000008174 sterile solution Substances 0.000 description 2
- 201000008827 tuberculosis Diseases 0.000 description 2
- 241000701242 Adenoviridae Species 0.000 description 1
- 208000010370 Adenoviridae Infections Diseases 0.000 description 1
- 206010060931 Adenovirus infection Diseases 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108091093088 Amplicon Proteins 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 241000557626 Corvus corax Species 0.000 description 1
- 238000011238 DNA vaccination Methods 0.000 description 1
- 108010053770 Deoxyribonucleases Proteins 0.000 description 1
- 102000016911 Deoxyribonucleases Human genes 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 101150066002 GFP gene Proteins 0.000 description 1
- 208000028523 Hereditary Complement Deficiency disease Diseases 0.000 description 1
- 102100021244 Integral membrane protein GPR180 Human genes 0.000 description 1
- 102100037850 Interferon gamma Human genes 0.000 description 1
- 102000008070 Interferon-gamma Human genes 0.000 description 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
- 208000002720 Malnutrition Diseases 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 241000223960 Plasmodium falciparum Species 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- 108091006629 SLC13A2 Proteins 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 102400000368 Surface protein Human genes 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 208000011589 adenoviridae infectious disease Diseases 0.000 description 1
- 108700010877 adenoviridae proteins Proteins 0.000 description 1
- 229940021704 adenovirus vaccine Drugs 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- ILRRQNADMUWWFW-UHFFFAOYSA-K aluminium phosphate Chemical compound O1[Al]2OP1(=O)O2 ILRRQNADMUWWFW-UHFFFAOYSA-K 0.000 description 1
- 229940001007 aluminium phosphate Drugs 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 210000001776 amniocyte Anatomy 0.000 description 1
- 239000003011 anion exchange membrane Substances 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 230000003466 anti-cipated effect Effects 0.000 description 1
- 230000005875 antibody response Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 238000011072 cell harvest Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000011097 chromatography purification Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000000432 density-gradient centrifugation Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 108010067396 dornase alfa Proteins 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 238000012395 formulation development Methods 0.000 description 1
- 229940044627 gamma-interferon Drugs 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 230000005745 host immune response Effects 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 229910052588 hydroxylapatite Inorganic materials 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 238000012007 large scale cell culture Methods 0.000 description 1
- 239000006193 liquid solution Substances 0.000 description 1
- 239000006194 liquid suspension Substances 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 230000002101 lytic effect Effects 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 239000012092 media component Substances 0.000 description 1
- 238000011140 membrane chromatography Methods 0.000 description 1
- 238000009285 membrane fouling Methods 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 235000021232 nutrient availability Nutrition 0.000 description 1
- 235000018343 nutrient deficiency Nutrition 0.000 description 1
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 229940124531 pharmaceutical excipient Drugs 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 230000000135 prohibitive effect Effects 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 229940107568 pulmozyme Drugs 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 210000001525 retina Anatomy 0.000 description 1
- 239000013017 sartobind Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000011146 sterile filtration Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000002305 strong-anion-exchange chromatography Methods 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 229960002109 tuberculosis vaccine Drugs 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 230000029812 viral genome replication Effects 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10341—Use of virus, viral particle or viral elements as a vector
- C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10351—Methods of production or purification of viral material
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Sustainable Development (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US19812208P | 2008-11-03 | 2008-11-03 | |
| EP08168181 | 2008-11-03 | ||
| EP08168181.9 | 2008-11-03 | ||
| US61/198,122 | 2008-11-03 | ||
| PCT/EP2009/064265 WO2010060719A1 (en) | 2008-11-03 | 2009-10-29 | Method for the production of adenoviral vectors |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2742474A1 CA2742474A1 (en) | 2010-06-03 |
| CA2742474C true CA2742474C (en) | 2016-05-31 |
Family
ID=40344776
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2742474A Active CA2742474C (en) | 2008-11-03 | 2009-10-29 | Method for the production of adenoviral vectors |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US10041049B2 (enExample) |
| EP (1) | EP2350268B1 (enExample) |
| JP (1) | JP5770633B2 (enExample) |
| KR (1) | KR101504392B1 (enExample) |
| CN (1) | CN102203242B (enExample) |
| AU (1) | AU2009319254B2 (enExample) |
| BR (1) | BRPI0921651A2 (enExample) |
| CA (1) | CA2742474C (enExample) |
| DK (1) | DK2350268T3 (enExample) |
| EA (1) | EA019928B1 (enExample) |
| ES (1) | ES2532015T3 (enExample) |
| MX (1) | MX2011004292A (enExample) |
| NZ (1) | NZ593235A (enExample) |
| PL (1) | PL2350268T3 (enExample) |
| WO (1) | WO2010060719A1 (enExample) |
Families Citing this family (55)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2532015T3 (es) | 2008-11-03 | 2015-03-23 | Crucell Holland B.V. | Método para la producción de vectores adenovíricos |
| MX2012004221A (es) | 2009-10-15 | 2012-06-08 | Crucell Holland Bv | Proceso para purificacion de adenovirus de cultivos de alta densidad celular. |
| WO2011045378A1 (en) | 2009-10-15 | 2011-04-21 | Crucell Holland B.V. | Method for the purification of adenovirus particles |
| ES2578514T3 (es) * | 2010-02-15 | 2016-07-27 | Crucell Holland B.V. | Método para la producción de vectores adenovíricos |
| CN103118702A (zh) | 2010-09-20 | 2013-05-22 | 克鲁塞尔荷兰公司 | 活动性结核病的治疗性接种 |
| AU2011310838B2 (en) | 2010-09-27 | 2015-11-05 | Crucell Holland B.V. | Heterologous prime boost vaccination regimen against malaria |
| PL2673072T3 (pl) | 2011-02-10 | 2015-04-30 | Crucell Holland Bv | System pneumatycznej zmiennociśnieniowej membranowej separacji komórek |
| EP2780034A1 (en) | 2011-11-14 | 2014-09-24 | Crucell Holland B.V. | Heterologous prime-boost immunization using measles virus-based vaccines |
| US8932607B2 (en) | 2012-03-12 | 2015-01-13 | Crucell Holland B.V. | Batches of recombinant adenovirus with altered terminal ends |
| ES2582504T3 (es) | 2012-03-12 | 2016-09-13 | Crucell Holland B.V. | Lotes de adenovirus recombinantes con extremos alterados |
| US9125870B2 (en) | 2012-03-22 | 2015-09-08 | Crucell Holland B.V. | Vaccine against RSV |
| MY169331A (en) | 2012-03-22 | 2019-03-21 | Janssen Vaccines & Prevention Bv | Vaccine against rsv |
| TR201902513T4 (tr) | 2013-04-25 | 2019-03-21 | Janssen Vaccines & Prevention Bv | Stabilize edilmiş çözünebilir prefüzyon RSV F polipeptitleri. |
| KR102313153B1 (ko) | 2013-06-17 | 2021-10-15 | 얀센 백신스 앤드 프리벤션 비.브이. | 안정화된 가용성 예비융합 rsv f 폴리펩타이드 |
| WO2015028546A1 (en) * | 2013-08-30 | 2015-03-05 | Glaxosmithkline Biologicals S.A. | Large scale production of viruses in cell culture |
| WO2015148704A1 (en) | 2014-03-25 | 2015-10-01 | Terumo Bct, Inc. | Passive replacement of media |
| JP6325751B2 (ja) | 2014-11-04 | 2018-05-16 | ヤンセン ファッシンズ アンド プリベンション ベーフェーJanssen Vaccines & Prevention B.V. | 治療用hpv16ワクチン |
| WO2017004592A1 (en) | 2015-07-02 | 2017-01-05 | Terumo Bct, Inc. | Cell growth with mechanical stimuli |
| WO2017005844A1 (en) | 2015-07-07 | 2017-01-12 | Janssen Vaccines & Prevention B.V. | Vaccine against rsv |
| US10457708B2 (en) | 2015-07-07 | 2019-10-29 | Janssen Vaccines & Prevention B.V. | Stabilized soluble pre-fusion RSV F polypeptides |
| US9663766B2 (en) | 2015-07-24 | 2017-05-30 | Bio-Rad Laboratories, Inc. | Methods for purifying adenovirus vectors |
| JP6470872B2 (ja) | 2015-08-20 | 2019-02-13 | ヤンセン ファッシンズ アンド プリベンション ベーフェーJanssen Vaccines & Prevention B.V. | 治療用hpv18ワクチン |
| KR102401247B1 (ko) | 2016-04-05 | 2022-05-25 | 얀센 백신스 앤드 프리벤션 비.브이. | Rsv에 대한 백신 |
| MY190320A (en) | 2016-04-05 | 2022-04-13 | Janssen Vaccines & Prevention Bv | Stabilized soluble pre-fusion rsv f proteins |
| WO2017180724A1 (en) * | 2016-04-12 | 2017-10-19 | Artemis Biosystems, Inc. | Perfusion filtration systems |
| SG11201808809PA (en) | 2016-05-02 | 2018-11-29 | Janssen Vaccine & Prevention B V | Therapeutic hpv vaccine combinations |
| WO2017205667A1 (en) | 2016-05-25 | 2017-11-30 | Terumo Bct, Inc. | Cell expansion |
| CA3025441A1 (en) | 2016-05-30 | 2017-12-07 | Janssen Vaccines & Prevention B.V. | Stabilized pre-fusion rsv f proteins |
| US11104874B2 (en) | 2016-06-07 | 2021-08-31 | Terumo Bct, Inc. | Coating a bioreactor |
| US11685883B2 (en) | 2016-06-07 | 2023-06-27 | Terumo Bct, Inc. | Methods and systems for coating a cell growth surface |
| JP7229151B2 (ja) | 2016-07-14 | 2023-02-27 | ヤンセン ファッシンズ アンド プリベンション ベーフェー | Hpvワクチン |
| US11629332B2 (en) | 2017-03-31 | 2023-04-18 | Terumo Bct, Inc. | Cell expansion |
| JP2020519663A (ja) | 2017-05-17 | 2020-07-02 | ヤンセン ファッシンズ アンド プリベンション ベーフェーJanssen Vaccines & Prevention B.V. | Rsv感染に対する防御免疫を誘導するための方法及び組成物 |
| KR20200053518A (ko) | 2017-09-15 | 2020-05-18 | 얀센 백신스 앤드 프리벤션 비.브이. | Rsv에 대한 면역의 안전한 유도를 위한 방법 |
| EA202091074A1 (ru) | 2017-10-31 | 2020-07-22 | Янссен Вэксинс Энд Превеншн Б.В. | Аденовирус и его применения |
| EP3704138A1 (en) | 2017-10-31 | 2020-09-09 | Janssen Vaccines & Prevention B.V. | Adenovirus and uses thereof |
| KR20200083510A (ko) | 2017-10-31 | 2020-07-08 | 얀센 백신스 앤드 프리벤션 비.브이. | 아데노바이러스 및 이의 용도 |
| MX421606B (es) | 2017-10-31 | 2025-03-14 | Janssen Vaccines & Prevention Bv | Vectores adenovirales y usos de estos |
| WO2019118426A1 (en) * | 2017-12-11 | 2019-06-20 | Amgen Inc. | Continuous manufacturing process for bispecific antibody products |
| CN110317832B (zh) | 2018-03-28 | 2022-07-05 | 西比曼生物科技(香港)有限公司 | Gmp级规模化制备重组慢病毒载体的纯化制剂的方法 |
| CN110317791A (zh) | 2018-03-29 | 2019-10-11 | 西比曼生物科技(香港)有限公司 | Gmp级无血清悬浮细胞大规模生产慢病毒的方法 |
| EP3784276A4 (en) | 2018-04-24 | 2022-03-16 | Merck Sharp & Dohme Corp. | SCALABLE CHROMATOGRAPHY PROCEDURE FOR PURIFICATION OF HUMAN CYTOMEGALOVIRUS |
| JOP20210106A1 (ar) | 2018-11-13 | 2023-01-30 | Janssen Vaccines And Prevention B V | بروتينات rsv f سابقة الاندماج مستقرة |
| AU2020300517A1 (en) * | 2019-07-01 | 2022-02-03 | Ology Bioservices, Inc. | Method for cultivation of adherent cells in a multiparallel bioreactor |
| BR112022014808A2 (pt) | 2020-01-31 | 2022-09-20 | Beth Israel Deaconess Medical Ct Inc | Composições e métodos para vacinas para prevenir e tratar infecção pelo coronavírus-sars-cov-2 |
| GB202013940D0 (en) | 2020-09-04 | 2020-10-21 | Synpromics Ltd | Regulatory nucleic acid sequences |
| IL301955A (en) | 2020-10-07 | 2023-06-01 | Asklepios Biopharmaceutical Inc | Therapeutic delivery of an adeno-related protein-related protein (FKRP) virus for the treatment of dystroglycanopathy disorders, including limb girdle 21 |
| WO2022207839A2 (en) | 2021-04-01 | 2022-10-06 | Janssen Vaccines & Prevention B.V. | Stabilized pre-fusion piv3 f proteins |
| WO2023020939A1 (en) | 2021-08-17 | 2023-02-23 | Janssen Pharmaceuticals, Inc. | Sars-cov-2 vaccines |
| WO2023111725A1 (en) | 2021-12-14 | 2023-06-22 | Janssen Pharmaceuticals, Inc. | Sars-cov-2 vaccines |
| WO2023110618A1 (en) | 2021-12-16 | 2023-06-22 | Janssen Vaccines & Prevention B.V. | Stabilized pre-fusion hmpv fusion proteins |
| EP4590690A1 (en) | 2022-09-23 | 2025-07-30 | Janssen Vaccines & Prevention B.V. | Pre-fusion human piv1 f proteins |
| WO2024061753A1 (en) | 2022-09-23 | 2024-03-28 | Janssen Vaccines & Prevention B.V. | Stabilized trimeric class i fusion proteins |
| JP2025533104A (ja) | 2022-10-06 | 2025-10-03 | エムエスディー インターナショナル ビジネス ゲーエムベーハー | 安定化融合前piv3 fタンパク質 |
| KR20250162763A (ko) | 2023-03-15 | 2025-11-19 | 도레이 카부시키가이샤 | 필터 유닛, 정제 장치 및 정제액의 제조 방법 |
Family Cites Families (73)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04241381A (ja) | 1991-01-16 | 1992-08-28 | Brother Ind Ltd | 電子学習機 |
| FR2705686B1 (fr) | 1993-05-28 | 1995-08-18 | Transgene Sa | Nouveaux adénovirus défectifs et lignées de complémentation correspondantes. |
| US5851806A (en) | 1994-06-10 | 1998-12-22 | Genvec, Inc. | Complementary adenoviral systems and cell lines |
| JP3816518B2 (ja) | 1994-06-10 | 2006-08-30 | ジェンベク、インコーポレイティッド | 相補的なアデノウイルスベクター系と細胞系 |
| US5965541A (en) | 1995-11-28 | 1999-10-12 | Genvec, Inc. | Vectors and methods for gene transfer to cells |
| US5846782A (en) | 1995-11-28 | 1998-12-08 | Genvec, Inc. | Targeting adenovirus with use of constrained peptide motifs |
| US5559099A (en) | 1994-09-08 | 1996-09-24 | Genvec, Inc. | Penton base protein and methods of using same |
| US5770442A (en) | 1995-02-21 | 1998-06-23 | Cornell Research Foundation, Inc. | Chimeric adenoviral fiber protein and methods of using same |
| US5837520A (en) | 1995-03-07 | 1998-11-17 | Canji, Inc. | Method of purification of viral vectors |
| DE69638058D1 (de) | 1995-06-15 | 2009-11-26 | Crucell Holland Bv | Verpackungssysteme für humane rekombinante Adenoviren zur Gentherapie |
| CA2230655C (en) | 1995-08-30 | 2008-06-17 | Genzyme Corporation | Chromatographic purification of adenovirus and aav |
| US5837511A (en) | 1995-10-02 | 1998-11-17 | Cornell Research Foundation, Inc. | Non-group C adenoviral vectors |
| CA2177085C (en) | 1996-04-26 | 2007-08-14 | National Research Council Of Canada | Adenovirus e1-complementing cell lines |
| US6485958B2 (en) | 1996-07-01 | 2002-11-26 | Gencell Sa | Method for producing recombinant adenovirus |
| FR2751343B1 (fr) | 1996-07-16 | 1998-12-18 | Transgene Sa | Procede de conservation de virus recombinants infectieux, suspension aqueuse virale et utilisation comme medicament |
| US7732129B1 (en) * | 1998-12-01 | 2010-06-08 | Crucell Holland B.V. | Method for the production and purification of adenoviral vectors |
| EP0968284B1 (en) | 1996-11-20 | 2006-12-13 | Introgen Therapeutics, Inc. | An improved method for the production and purification of adenoviral vectors |
| US6261823B1 (en) | 1996-12-13 | 2001-07-17 | Schering Corporation | Methods for purifying viruses |
| JP2000509614A (ja) | 1997-03-04 | 2000-08-02 | バクスター インターナショナル インコーポレイテッド | アデノウイルスe1−相補性細胞系 |
| US6020191A (en) | 1997-04-14 | 2000-02-01 | Genzyme Corporation | Adenoviral vectors capable of facilitating increased persistence of transgene expression |
| US6210683B1 (en) | 1997-09-05 | 2001-04-03 | Merck & Co., Inc. | Stabilizers containing recombinant human serum albumin for live virus vaccines |
| JP4358434B2 (ja) | 1998-02-17 | 2009-11-04 | シェーリング コーポレイション | ウイルスを含む組成物、およびウイルス調製物を濃縮するための方法 |
| US5981225A (en) | 1998-04-16 | 1999-11-09 | Baylor College Of Medicine | Gene transfer vector, recombinant adenovirus particles containing the same, method for producing the same and method of use of the same |
| EA200001096A1 (ru) | 1998-04-22 | 2001-04-23 | Джинвек, Инк. | Способ обогащения раствора аденовируса, способ точного подсчета числа аденовирусных частиц в образце раствора (варианты) и способ очистки аденовируса из клеток, инфицированных аденовирусом |
| US6113913A (en) | 1998-06-26 | 2000-09-05 | Genvec, Inc. | Recombinant adenovirus |
| US20040043489A1 (en) | 1998-07-08 | 2004-03-04 | Menzo Havenga | Gene delivery vectors provided with a tissue tropism for dendritic cells and methods of use |
| US20030017138A1 (en) | 1998-07-08 | 2003-01-23 | Menzo Havenga | Chimeric adenoviruses |
| EP1133316B1 (en) | 1998-11-16 | 2009-01-21 | Introgen Therapeutics, Inc. | Formulation of adenovirus for gene therapy |
| NZ512504A (en) | 1998-12-31 | 2004-01-30 | Aventis Pharma Sa | Method for separating viral particles |
| WO2000050573A1 (fr) | 1999-02-22 | 2000-08-31 | Transgene S.A. | Procede d'obtention d'une preparation virale purifiee |
| ATE519855T1 (de) | 1999-05-17 | 2011-08-15 | Crucell Holland Bv | Rekombinantes adenovirus des serotyps ad11 |
| US6913922B1 (en) | 1999-05-18 | 2005-07-05 | Crucell Holland B.V. | Serotype of adenovirus and uses thereof |
| US20050232900A1 (en) | 1999-05-18 | 2005-10-20 | Crucell Holland B.V. | Serotype of adenovirus and uses thereof |
| US6492169B1 (en) | 1999-05-18 | 2002-12-10 | Crucell Holland, B.V. | Complementing cell lines |
| DE19955558C2 (de) | 1999-11-18 | 2003-03-20 | Stefan Kochanek | Permanente Amniozyten-Zelllinie, ihre Herstellung und Verwendung zur Herstellung von Gentransfervektoren |
| US6544424B1 (en) | 1999-12-03 | 2003-04-08 | Refined Technology Company | Fluid filtration system |
| WO2001066137A1 (en) | 2000-03-07 | 2001-09-13 | Merck & Co., Inc. | Adenovirus formulations |
| DE60138403D1 (de) | 2000-09-26 | 2009-05-28 | Crucell Holland Bv | Adenovirale vektoren für die übertragung von genen in zellen der skelettmuskulatur oder myoblasten |
| US20020064860A1 (en) | 2000-11-29 | 2002-05-30 | Schering Corporation | Method for purifying adenoviruses |
| EP1453537A1 (en) | 2001-12-12 | 2004-09-08 | FH Faulding & Co. Limited | Composition for viral preservation |
| WO2003062400A2 (en) | 2002-01-24 | 2003-07-31 | The Scripps Research Institute | Fiber shaft modifications for efficient targeting |
| US20030180936A1 (en) | 2002-03-15 | 2003-09-25 | Memarzadeh Bahram Eric | Method for the purification, production and formulation of oncolytic adenoviruses |
| PL208588B1 (pl) | 2002-04-25 | 2011-05-31 | Crucell Holland Bv | Rekombinowane adenowirusy, wyizolowane kwasy nukleinowe, komórki pakujące oraz sposoby zwiększania stabilności i/lub pojemności upakowania rekombinowanego adenowirusa |
| NZ534866A (en) | 2002-04-25 | 2007-04-27 | Crucell Holland B | Means and methods for the production of adenovirus vectors |
| EP1783212B1 (en) | 2002-05-14 | 2011-01-05 | Merck Sharp & Dohme Corp. | Methods of adenovirus purification |
| US20080153083A1 (en) | 2003-10-23 | 2008-06-26 | Crucell Holland B.V. | Settings for recombinant adenoviral-based vaccines |
| AU2003288273A1 (en) | 2002-10-23 | 2004-05-13 | Crucell Holland B.V. | New settings for recombinant adenoviral-based vaccines |
| AU2003298361B2 (en) | 2002-12-17 | 2009-05-14 | Crucell Holland B.V. | Recombinant viral-based malaria vaccines |
| CA2519680A1 (en) | 2003-03-28 | 2004-11-18 | The Scripps Research Institute | Adenovirus particles with enhanced infectivity of dendritic cells and particles with decreased infectivity of hepatocytes |
| CN1863918B (zh) | 2003-10-02 | 2011-03-30 | 克鲁塞尔荷兰公司 | 用于重组腺病毒的包装细胞 |
| CA2555412C (en) | 2004-02-23 | 2013-06-25 | Crucell Holland B.V. | Virus purification methods |
| KR101050585B1 (ko) * | 2004-03-05 | 2011-07-19 | 디에스엠 아이피 어셋츠 비.브이. | 연속식 살포 및 교호 접선 흐름에 의한 세포 배양 방법 |
| ES2612212T5 (es) | 2004-06-04 | 2020-06-25 | Global Life Sciences Solutions Usa Llc | Sistemas y métodos de biorreactor desechable |
| AU2005293568B2 (en) | 2004-10-13 | 2010-10-28 | Beth Israel Deaconess Medical Center Inc. | Improved adenoviral vectors and uses thereof |
| AU2005293572B2 (en) | 2004-10-14 | 2011-08-04 | Crucell Holland B.V. | Malaria prime/boost vaccines |
| CN101090974B (zh) | 2004-11-16 | 2011-05-11 | 克鲁塞尔荷兰公司 | 包含重组病毒载体的多价疫苗 |
| AU2006212885A1 (en) | 2005-02-11 | 2006-08-17 | Merck & Co., Inc. | Adenovirus serotype 26 vectors, nucleic acid and viruses produced thereby |
| ATE412737T1 (de) | 2005-04-11 | 2008-11-15 | Crucell Holland Bv | Virusreinigung mit ultrafiltration |
| JP2008541730A (ja) | 2005-05-23 | 2008-11-27 | ヴァクシン インコーポレイテッド | 高力価かつ複製コンピテントアデノウイルス不含である組換えアデノウイルスベクターの迅速な作成法 |
| US20100115874A1 (en) | 2005-07-22 | 2010-05-13 | Pergo (Europe) Ab | Flooring system including a dry glue |
| KR101345936B1 (ko) | 2005-08-15 | 2013-12-31 | 박신 인크. | 비복제성 벡터화된 백신 투여에 의한 조류 면역화 |
| WO2007104792A2 (en) | 2006-03-16 | 2007-09-20 | Crucell Holland B.V. | Recombinant adenoviruses based on serotype 26 and 48, and use thereof |
| US20090110695A1 (en) | 2006-03-27 | 2009-04-30 | Menzo Jans Emko Havenga | Compositions Comprising a Recombinant Adenovirus and an Adjuvant |
| SG173363A1 (en) | 2006-07-18 | 2011-08-29 | Glaxosmithkline Biolog Sa | Vaccines for malaria |
| EA021391B1 (ru) | 2007-03-02 | 2015-06-30 | Глаксосмитклайн Байолоджикалс С.А. | Способ индукции иммунного ответа, вакцинная композиция, ее применение и набор |
| US9109193B2 (en) | 2007-07-30 | 2015-08-18 | Ge Healthcare Bio-Sciences Corp. | Continuous perfusion bioreactor system |
| EP2209925A4 (en) | 2007-11-14 | 2017-11-22 | Chun-Chieh Chang | Method and devices for producing air sensitive electrode materials for lithium ion battery applications |
| WO2009117134A2 (en) | 2008-03-21 | 2009-09-24 | National Institutes Of Health | Aerosolized genetic vaccines and methods of use |
| ES2532015T3 (es) | 2008-11-03 | 2015-03-23 | Crucell Holland B.V. | Método para la producción de vectores adenovíricos |
| EP2454364B1 (en) | 2009-07-16 | 2014-04-23 | Crucell Holland B.V. | Production of polio virus at high titers for vaccine production |
| MX2012004221A (es) | 2009-10-15 | 2012-06-08 | Crucell Holland Bv | Proceso para purificacion de adenovirus de cultivos de alta densidad celular. |
| US20120315696A1 (en) | 2010-02-15 | 2012-12-13 | Alfred Luitjens | METHOD FOR THE PRODUCTION OF Ad26 ADENOVIRAL VECTORS |
| ES2578514T3 (es) | 2010-02-15 | 2016-07-27 | Crucell Holland B.V. | Método para la producción de vectores adenovíricos |
-
2009
- 2009-10-29 ES ES09744140.6T patent/ES2532015T3/es active Active
- 2009-10-29 DK DK09744140.6T patent/DK2350268T3/en active
- 2009-10-29 EP EP09744140.6A patent/EP2350268B1/en active Active
- 2009-10-29 EA EA201170638A patent/EA019928B1/ru not_active IP Right Cessation
- 2009-10-29 AU AU2009319254A patent/AU2009319254B2/en not_active Ceased
- 2009-10-29 US US13/126,978 patent/US10041049B2/en active Active
- 2009-10-29 MX MX2011004292A patent/MX2011004292A/es active IP Right Grant
- 2009-10-29 PL PL09744140T patent/PL2350268T3/pl unknown
- 2009-10-29 JP JP2011533718A patent/JP5770633B2/ja not_active Expired - Fee Related
- 2009-10-29 CN CN2009801438951A patent/CN102203242B/zh not_active Expired - Fee Related
- 2009-10-29 WO PCT/EP2009/064265 patent/WO2010060719A1/en not_active Ceased
- 2009-10-29 BR BRPI0921651-0A patent/BRPI0921651A2/pt not_active Application Discontinuation
- 2009-10-29 KR KR1020117010793A patent/KR101504392B1/ko not_active Expired - Fee Related
- 2009-10-29 CA CA2742474A patent/CA2742474C/en active Active
- 2009-10-29 NZ NZ593235A patent/NZ593235A/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| US10041049B2 (en) | 2018-08-07 |
| NZ593235A (en) | 2013-02-22 |
| WO2010060719A1 (en) | 2010-06-03 |
| MX2011004292A (es) | 2011-05-31 |
| CN102203242A (zh) | 2011-09-28 |
| JP5770633B2 (ja) | 2015-08-26 |
| EA201170638A1 (ru) | 2011-12-30 |
| JP2012507270A (ja) | 2012-03-29 |
| BRPI0921651A2 (pt) | 2015-08-18 |
| KR101504392B1 (ko) | 2015-03-19 |
| DK2350268T3 (en) | 2015-03-23 |
| CA2742474A1 (en) | 2010-06-03 |
| CN102203242B (zh) | 2013-06-12 |
| EA019928B1 (ru) | 2014-07-30 |
| PL2350268T3 (pl) | 2015-05-29 |
| HK1160664A1 (en) | 2012-08-10 |
| US20110207202A1 (en) | 2011-08-25 |
| AU2009319254A1 (en) | 2010-06-03 |
| KR20110093793A (ko) | 2011-08-18 |
| ES2532015T3 (es) | 2015-03-23 |
| EP2350268A1 (en) | 2011-08-03 |
| EP2350268B1 (en) | 2014-12-24 |
| AU2009319254B2 (en) | 2015-04-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2742474C (en) | Method for the production of adenoviral vectors | |
| CA2786835C (en) | Method for the production of ad26 adenoviral vectors | |
| US20180080010A1 (en) | Method for the production of ad26 adenoviral vectors | |
| US8124106B2 (en) | Virus purification methods | |
| US8574595B2 (en) | Virus purification using ultrafiltration | |
| HK1160664B (en) | Method for the production of adenoviral vectors | |
| HK1180008B (en) | Method for the production of ad26 adenoviral vectors |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20140610 |