CA2456140C - Methodes de detection d'adn provenant de differents individus - Google Patents

Methodes de detection d'adn provenant de differents individus Download PDF

Info

Publication number
CA2456140C
CA2456140C CA2456140A CA2456140A CA2456140C CA 2456140 C CA2456140 C CA 2456140C CA 2456140 A CA2456140 A CA 2456140A CA 2456140 A CA2456140 A CA 2456140A CA 2456140 C CA2456140 C CA 2456140C
Authority
CA
Canada
Prior art keywords
dna
reagent
dna species
biological sample
methylation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2456140A
Other languages
English (en)
Other versions
CA2456140A1 (fr
Inventor
Yuk Ming Dennis Lo
Lit Man Poon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chinese University of Hong Kong CUHK
Original Assignee
Chinese University of Hong Kong CUHK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chinese University of Hong Kong CUHK filed Critical Chinese University of Hong Kong CUHK
Priority to CA2887218A priority Critical patent/CA2887218C/fr
Publication of CA2456140A1 publication Critical patent/CA2456140A1/fr
Application granted granted Critical
Publication of CA2456140C publication Critical patent/CA2456140C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)

Abstract

Dans un premier aspect, la présente invention concerne des méthodes de différenciation de types d'ADN provenant de différents individus dans un prélèvement biologique. Ces méthodes peuvent être utilisées pour différencier ou détecter l'ADN foetal dans un prélèvement maternel ou pour différencier l'ADN d'un donneur d'organe de celui d'un receveur d'organe. Dans des modes de réalisation préférés, on différencie ces types d'ADN en observant des différences épigénétiques dans ces types d'ADN telles que des différences en matière de méthylation de l'ADN. Dans un deuxième aspect, la présente invention concerne des méthodes de détection d'anomalies génétiques chez un foetus par détection de l'ADN foetal dans un prélèvement biologique provenant d'une mère. Dans un troisième aspect, ladite invention concerne des méthodes permettant de différencier des types d'ADN provenant d'un donneur d'organe de ceux provenant d'un receveur d'organe. Dans un quatrième aspect, cette invention concerne des trousses permettant de différencier des types d'ADN provenant de différents individus dans un prélèvement biologique.
CA2456140A 2001-08-31 2002-08-30 Methodes de detection d'adn provenant de differents individus Expired - Lifetime CA2456140C (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2887218A CA2887218C (fr) 2001-08-31 2002-08-30 Systeme d'amplification d'une espece d'adn foetal

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/944,951 2001-08-31
US09/944,951 US6927028B2 (en) 2001-08-31 2001-08-31 Non-invasive methods for detecting non-host DNA in a host using epigenetic differences between the host and non-host DNA
PCT/GB2002/003941 WO2003020974A2 (fr) 2001-08-31 2002-08-30 Methodes de detection d'adn provenant de differents individus

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA2887218A Division CA2887218C (fr) 2001-08-31 2002-08-30 Systeme d'amplification d'une espece d'adn foetal

Publications (2)

Publication Number Publication Date
CA2456140A1 CA2456140A1 (fr) 2003-03-13
CA2456140C true CA2456140C (fr) 2015-06-23

Family

ID=25482355

Family Applications (4)

Application Number Title Priority Date Filing Date
CA3012120A Expired - Lifetime CA3012120C (fr) 2001-08-31 2002-08-30 Systeme d'amplification d'especes d'adn foetales
CA2456140A Expired - Lifetime CA2456140C (fr) 2001-08-31 2002-08-30 Methodes de detection d'adn provenant de differents individus
CA2973165A Expired - Lifetime CA2973165C (fr) 2001-08-31 2002-08-30 Systeme d'amplification d'especes d'adn foetales
CA2887218A Expired - Lifetime CA2887218C (fr) 2001-08-31 2002-08-30 Systeme d'amplification d'une espece d'adn foetal

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CA3012120A Expired - Lifetime CA3012120C (fr) 2001-08-31 2002-08-30 Systeme d'amplification d'especes d'adn foetales

Family Applications After (2)

Application Number Title Priority Date Filing Date
CA2973165A Expired - Lifetime CA2973165C (fr) 2001-08-31 2002-08-30 Systeme d'amplification d'especes d'adn foetales
CA2887218A Expired - Lifetime CA2887218C (fr) 2001-08-31 2002-08-30 Systeme d'amplification d'une espece d'adn foetal

Country Status (13)

Country Link
US (7) US6927028B2 (fr)
EP (1) EP1421211B1 (fr)
JP (1) JP2005501566A (fr)
CN (1) CN100352939C (fr)
AT (1) ATE353977T1 (fr)
AU (1) AU2002324135A1 (fr)
CA (4) CA3012120C (fr)
DE (1) DE60218182T2 (fr)
DK (1) DK1421211T3 (fr)
ES (1) ES2280562T3 (fr)
HK (1) HK1076840A1 (fr)
PT (1) PT1421211E (fr)
WO (1) WO2003020974A2 (fr)

Families Citing this family (189)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6927028B2 (en) * 2001-08-31 2005-08-09 Chinese University Of Hong Kong Non-invasive methods for detecting non-host DNA in a host using epigenetic differences between the host and non-host DNA
EP1468104A4 (fr) * 2002-01-18 2006-02-01 Genzyme Corp Procedes de detection d'adn foetal et de quantification d'alleles
WO2003074740A1 (fr) * 2002-03-01 2003-09-12 Ravgen, Inc. Analyse rapide de variations dans un genome
US6977162B2 (en) * 2002-03-01 2005-12-20 Ravgen, Inc. Rapid analysis of variations in a genome
DK1342794T3 (da) * 2002-03-05 2006-04-24 Epigenomics Ag Fremgangsmåde og anordning til at bestemme vævsspecificitet af fritflydende DNA i legemsvæsker
US7442506B2 (en) * 2002-05-08 2008-10-28 Ravgen, Inc. Methods for detection of genetic disorders
US7727720B2 (en) * 2002-05-08 2010-06-01 Ravgen, Inc. Methods for detection of genetic disorders
US20070178478A1 (en) * 2002-05-08 2007-08-02 Dhallan Ravinder S Methods for detection of genetic disorders
WO2004078999A1 (fr) * 2003-03-05 2004-09-16 Genetic Technologies Limited Identification d'adn foetal et de marqueurs de cellule foetale dans du plasma ou du serum maternel
AU2004274724B2 (en) * 2003-09-22 2009-09-10 Trisogen Biotechnology Limited Partnership Methods and kits useful for detecting an alteration in a locus copy number
US20050282213A1 (en) * 2003-09-22 2005-12-22 Trisogen Biotechnology Limited Partnership Methods and kits useful for detecting an alteration in a locus copy number
WO2005035725A2 (fr) * 2003-10-08 2005-04-21 The Trustees Of Boston University Methodes de diagnostic prenatal d'anomalies chromosomiques
US8529744B2 (en) 2004-02-02 2013-09-10 Boreal Genomics Corp. Enrichment of nucleic acid targets
CA2552262C (fr) 2004-02-02 2016-04-19 The University Of British Columbia Scodaphorese, procedes et appareil utilises pour deplacer et concentrer des particules
US10337054B2 (en) 2004-02-02 2019-07-02 Quantum-Si Incorporated Enrichment of nucleic acid targets
US7709194B2 (en) 2004-06-04 2010-05-04 The Chinese University Of Hong Kong Marker for prenatal diagnosis and monitoring
JP5133238B2 (ja) 2005-04-15 2013-01-30 エピゲノミックス アクチェンゲゼルシャフト 遠隔サンプル由来のｄｎａ断片を提供する方法
US20090317798A1 (en) * 2005-06-02 2009-12-24 Heid Christian A Analysis using microfluidic partitioning devices
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US11111543B2 (en) * 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
PL3002338T3 (pl) * 2006-02-02 2019-12-31 The Board Of Trustees Of The Leland Stanford Junior University Nieinwazyjne badania przesiewowe płodu poprzez analizę cyfrową
EP1994164A4 (fr) * 2006-03-06 2010-07-21 Univ Columbia Amplification spécifique de séquences d'adn foetal à partir d'une source maternelle foetale, mélangée
US7901884B2 (en) * 2006-05-03 2011-03-08 The Chinese University Of Hong Kong Markers for prenatal diagnosis and monitoring
US7754428B2 (en) * 2006-05-03 2010-07-13 The Chinese University Of Hong Kong Fetal methylation markers
US8679741B2 (en) 2006-05-31 2014-03-25 Sequenom, Inc. Methods and compositions for the extraction and amplification of nucleic acid from a sample
US20080050739A1 (en) * 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
WO2007147074A2 (fr) * 2006-06-14 2007-12-21 Living Microsystems, Inc. Utilisation de génotypage snp fortement parallèle pour diagnostic fœtal
US8137912B2 (en) * 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
US8372584B2 (en) 2006-06-14 2013-02-12 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
TWI335354B (en) 2006-09-27 2011-01-01 Univ Hong Kong Chinese Methods for the detection of the degree of the methylation of a target dna and kits
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
JP5211790B2 (ja) * 2007-03-26 2013-06-12 住友化学株式会社 Ｄｎａメチル化測定方法
ITTO20070307A1 (it) * 2007-05-04 2008-11-05 Silicon Biosystems Spa Metodo e dispositivo per la diagnosi prenatale non-invasiva
ES2570828T3 (es) 2007-06-08 2016-05-20 Epigenomics Ag Método de análisis de metilación
EP3770275A1 (fr) 2007-07-23 2021-01-27 The Chinese University of Hong Kong Détermination d'une aneuploïdie fétale
US20100112590A1 (en) 2007-07-23 2010-05-06 The Chinese University Of Hong Kong Diagnosing Fetal Chromosomal Aneuploidy Using Genomic Sequencing With Enrichment
AU2013202160B2 (en) * 2007-07-23 2015-07-23 The Chinese University Of Hong Kong Determining percentage of fetal dna in maternal sample
US9404150B2 (en) 2007-08-29 2016-08-02 Sequenom, Inc. Methods and compositions for universal size-specific PCR
BR122018069446B8 (pt) 2008-01-18 2021-07-27 Harvard College método in vitro para detectar a presença de um célula de câncer em um indivíduo
EP2238434A4 (fr) 2008-02-01 2011-06-22 Univ British Columbia Procédés et appareil pour l'introduction et la récupération de particules
CA2752838A1 (fr) * 2008-02-18 2009-08-27 Genetic Technologies Limited Procedes de traitement et/ou d'enrichissement de cellules
AU2009223671B2 (en) * 2008-03-11 2014-11-27 Sequenom, Inc. Nucleic acid-based tests for prenatal gender determination
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
ES2599967T3 (es) 2008-09-16 2017-02-06 Sequenom, Inc. Procedimientos y composiciones para el enriquecimiento basado en metilación de ácido nucleico fetal de una muestra materna útiles para diagnósticos prenatales no invasivos
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
LT2562268T (lt) 2008-09-20 2017-04-25 The Board Of Trustees Of The Leland Stanford Junior University Neinvazinis fetalinės aneuploidijos diagnozavimas sekvenavimu
WO2010107946A2 (fr) * 2009-03-18 2010-09-23 Sequenom, Inc. Utilisation d'endonucléases thermostables pour générer des molécules rapporteuses
EP3514244B1 (fr) 2009-04-03 2021-07-07 Sequenom, Inc. Procédés de préparation d'acides nucléiques
WO2010121294A1 (fr) 2009-04-21 2010-10-28 Genetic Technologies Limited Procédés d'obtention de matériel génétique foetal
WO2010121381A1 (fr) 2009-04-21 2010-10-28 The University Of British Columbia Système et procédés pour la détection de particules
CA2767616A1 (fr) 2009-07-09 2011-01-13 The Scripps Research Institute Profils d'expression genique associes a une nephropathie chronique de l'allogreffe
HUE061110T2 (hu) 2009-11-05 2023-05-28 Univ Hong Kong Chinese Magzati genomelemzés anyai biológiai mintából
US8620593B2 (en) 2009-11-06 2013-12-31 The Chinese University Of Hong Kong Size-based genomic analysis
DK2496720T3 (da) 2009-11-06 2020-09-28 Univ Leland Stanford Junior Ikke-invasiv diagnose af transplantatafstødning i organ-transplanterede patienter
EP2504448B1 (fr) * 2009-11-25 2016-10-19 Bio-Rad Laboratories, Inc. Procédés et compositions destinés à détecter un matériel génétique
WO2011087760A2 (fr) 2009-12-22 2011-07-21 Sequenom, Inc. Procédés et kits pour identifier une aneuploïdie
US10388403B2 (en) 2010-01-19 2019-08-20 Verinata Health, Inc. Analyzing copy number variation in the detection of cancer
ES2704701T3 (es) 2010-01-19 2019-03-19 Verinata Health Inc Nuevo protocolo de preparación de bibliotecas de secuenciación
US9323888B2 (en) 2010-01-19 2016-04-26 Verinata Health, Inc. Detecting and classifying copy number variation
EP2526415B1 (fr) 2010-01-19 2017-05-03 Verinata Health, Inc Procédés de détection définis par des partitions
US9260745B2 (en) 2010-01-19 2016-02-16 Verinata Health, Inc. Detecting and classifying copy number variation
US20120010085A1 (en) 2010-01-19 2012-01-12 Rava Richard P Methods for determining fraction of fetal nucleic acids in maternal samples
AU2011207544A1 (en) * 2010-01-19 2012-09-06 Verinata Health, Inc. Identification of polymorphic sequences in mixtures of genomic DNA by whole genome sequencing
US20120100548A1 (en) 2010-10-26 2012-04-26 Verinata Health, Inc. Method for determining copy number variations
US20110312503A1 (en) 2010-01-23 2011-12-22 Artemis Health, Inc. Methods of fetal abnormality detection
US8774488B2 (en) 2010-03-11 2014-07-08 Cellscape Corporation Method and device for identification of nucleated red blood cells from a maternal blood sample
EP2854057B1 (fr) 2010-05-18 2018-03-07 Natera, Inc. Procédés pour une classification de ploïdie prénatale non invasive
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
KR20130041961A (ko) 2010-07-23 2013-04-25 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 체액에서 질환 또는 상태의 특징을 검출하는 방법
WO2012012717A1 (fr) 2010-07-23 2012-01-26 President And Fellows Of Harvard College Méthodes de dépistage de maladies ou d'affections prénatales ou liées à la grossesse
AU2011280997A1 (en) 2010-07-23 2013-02-28 President And Fellows Of Harvard College Methods of detecting autoimmune or immune-related diseases or conditions
KR20130041962A (ko) 2010-07-23 2013-04-25 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 식작용 세포를 사용하여 질환 또는 상태를 검출하는 방법
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US20130040375A1 (en) 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US10167508B2 (en) 2010-08-06 2019-01-01 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US11031095B2 (en) 2010-08-06 2021-06-08 Ariosa Diagnostics, Inc. Assay systems for determination of fetal copy number variation
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
US9267174B2 (en) 2010-10-26 2016-02-23 Stanford University Method of simultaneously screening for multiple genotypes and/or mutations
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
US8756020B2 (en) 2011-01-25 2014-06-17 Ariosa Diagnostics, Inc. Enhanced risk probabilities using biomolecule estimations
JP6153874B2 (ja) 2011-02-09 2017-06-28 ナテラ， インコーポレイテッド 非侵襲的出生前倍数性呼び出しのための方法
EP3940084A1 (fr) 2011-02-09 2022-01-19 Bio-Rad Laboratories, Inc. Analyse d'acides nucléiques
TWI611186B (zh) 2011-02-24 2018-01-11 香港中文大學 多重妊娠之分子檢驗
DK3567124T3 (da) * 2011-04-12 2022-03-07 Verinata Health Inc Opløsning af genomfraktioner ved anvendelse af polymorfisme-optællinger
US9411937B2 (en) 2011-04-15 2016-08-09 Verinata Health, Inc. Detecting and classifying copy number variation
CA2834218C (fr) 2011-04-29 2021-02-16 Sequenom, Inc. Quantification d'une minorite d'especes d'acide nucleique utilisant des oligonucleotides inhibiteurs
CA2742327A1 (fr) 2011-05-20 2012-11-20 The University Of British Columiba Systemes et methodes pour amelioration de la scoda (separation, concentration, detection de molecules selon la difference d'affinite de liaison)
US20140235474A1 (en) 2011-06-24 2014-08-21 Sequenom, Inc. Methods and processes for non invasive assessment of a genetic variation
US8712697B2 (en) 2011-09-07 2014-04-29 Ariosa Diagnostics, Inc. Determination of copy number variations using binomial probability calculations
US10424394B2 (en) 2011-10-06 2019-09-24 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10196681B2 (en) 2011-10-06 2019-02-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9984198B2 (en) 2011-10-06 2018-05-29 Sequenom, Inc. Reducing sequence read count error in assessment of complex genetic variations
US20140242588A1 (en) 2011-10-06 2014-08-28 Sequenom, Inc Methods and processes for non-invasive assessment of genetic variations
JP6073902B2 (ja) 2011-10-06 2017-02-01 セクエノム， インコーポレイテッド 遺伝的変異の非侵襲的評価のための方法およびプロセス
US9367663B2 (en) 2011-10-06 2016-06-14 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CA2851537C (fr) 2011-10-11 2020-12-29 Sequenom, Inc. Methodes et procedes d'evaluation non invasive de variations genetiques
US8688388B2 (en) 2011-10-11 2014-04-01 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
WO2013104994A2 (fr) 2012-01-13 2013-07-18 The University Of British Columbia Appareil à bras multiples et procédés pour la séparation de particules
EP2805280B1 (fr) 2012-01-20 2022-10-05 Sequenom, Inc. Processus de diagnostic qui tiennent compte des conditions d'expérimentation
ES2930180T3 (es) 2012-03-02 2022-12-07 Sequenom Inc Métodos para enriquecer ácido nucleico canceroso a partir de una muestra biológica
EP3573066B1 (fr) 2012-03-13 2023-09-27 The Chinese University Of Hong Kong Procédés d'analyse de données de séquençage massivement parallèles pour diagnostic prénatal non invasif
US9512477B2 (en) 2012-05-04 2016-12-06 Boreal Genomics Inc. Biomarker anaylsis using scodaphoresis
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
US10289800B2 (en) 2012-05-21 2019-05-14 Ariosa Diagnostics, Inc. Processes for calculating phased fetal genomic sequences
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
JP2015519900A (ja) 2012-05-21 2015-07-16 フリューダイム・コーポレイション 粒子集団の単粒子解析方法及び単粒子単離方法
DK3663409T3 (da) 2012-05-21 2021-12-13 Sequenom Inc Fremgangsmåder og processer til ikke-invasiv bedømmelse af genetiske variationer
US10497461B2 (en) 2012-06-22 2019-12-03 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CA2878979C (fr) 2012-07-13 2021-09-14 Sequenom, Inc. Procedes et compositions pour enrichissement base sur la methylation en acide nucleique foetal dans un echantillon maternel, utiles pour les diagnostics prenatals non invasifs
CN104583421A (zh) 2012-07-19 2015-04-29 阿瑞奥萨诊断公司 遗传变体的基于多重的顺序连接的检测
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
LT3354747T (lt) 2012-09-20 2021-04-12 The Chinese University Of Hong Kong Neinvazinis naviko metilomos nustatymas iš plazmos
US10706957B2 (en) 2012-09-20 2020-07-07 The Chinese University Of Hong Kong Non-invasive determination of methylome of tumor from plasma
US9732390B2 (en) 2012-09-20 2017-08-15 The Chinese University Of Hong Kong Non-invasive determination of methylome of fetus or tumor from plasma
US10482994B2 (en) 2012-10-04 2019-11-19 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CA3120521A1 (fr) 2012-10-04 2014-04-10 Sequenom, Inc. Procedes et processus d'evaluation non invasive de variations genetiques
US20130309666A1 (en) 2013-01-25 2013-11-21 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP2965086A4 (fr) 2013-03-09 2017-02-08 Harry Stylli Procédés de détection du cancer de la prostate
EP4202441A3 (fr) 2013-03-09 2023-07-26 Immunis.AI, Inc. Profil d'expression génétique dans les macrophages pour le diagnostic du cancer
US20130189684A1 (en) 2013-03-12 2013-07-25 Sequenom, Inc. Quantification of cell-specific nucleic acid markers
WO2014168711A1 (fr) 2013-03-13 2014-10-16 Sequenom, Inc. Amorces pour analyse de la méthylation de l'adn
WO2014144713A2 (fr) 2013-03-15 2014-09-18 Immumetrix, Inc. Méthode de séquençage du répertoire immunitaire
US9340835B2 (en) 2013-03-15 2016-05-17 Boreal Genomics Corp. Method for separating homoduplexed and heteroduplexed nucleic acids
JP2016518811A (ja) 2013-03-15 2016-06-30 ザ チャイニーズ ユニバーシティ オブ ホンコン 多胎妊娠における胎児ゲノムの決定
HUE061261T2 (hu) 2013-04-03 2023-05-28 Sequenom Inc Eljárások és folyamatok genetikai variánsok nem invazív értékelésére
JP6561046B2 (ja) 2013-05-24 2019-08-14 セクエノム， インコーポレイテッド 遺伝子の変動の非侵襲性評価のための方法および処理
SI3011051T1 (sl) 2013-06-21 2019-05-31 Sequenom, Inc. Postopek za neinvazivno oceno genetskih variacij
ES2685893T3 (es) * 2013-07-30 2018-10-15 Bgi Genomics Co., Limited Método para determinar la composición de ácidos nucleicos de una mezcla de ácidos nucleicos
IL289974B (en) 2013-10-04 2022-09-01 Sequenom Inc Methods and processes for non-invasive evaluation of genetic variations
JP6680680B2 (ja) 2013-10-07 2020-04-15 セクエノム， インコーポレイテッド 染色体変化の非侵襲性評価のための方法およびプロセス
EP3736344A1 (fr) 2014-03-13 2020-11-11 Sequenom, Inc. Méthodes et procédés d'évaluation non invasive de variations génétiques
CN106460070B (zh) 2014-04-21 2021-10-08 纳特拉公司 检测染色体片段中的突变和倍性
EP2942400A1 (fr) 2014-05-09 2015-11-11 Lifecodexx AG Détection multiplexe d'ADN qui provient d'un type cellulaire spécifique
WO2015169947A1 (fr) 2014-05-09 2015-11-12 Lifecodexx Ag Détection de l'adn provenant d'un type spécifique de cellule et méthodes associées
US11104951B2 (en) 2014-05-22 2021-08-31 The Scripps Research Institute Molecular signatures for distinguishing liver transplant rejections or injuries
US10443100B2 (en) 2014-05-22 2019-10-15 The Scripps Research Institute Gene expression profiles associated with sub-clinical kidney transplant rejection
WO2015183872A1 (fr) 2014-05-30 2015-12-03 Sequenom, Inc. Déterminations de représentation de chromosomes
US20170211143A1 (en) 2014-07-25 2017-07-27 University Of Washington Methods of determining tissues and/or cell types giving rise to cell-free dna, and methods of identifying a disease or disorder using same
EP3175000B1 (fr) 2014-07-30 2020-07-29 Sequenom, Inc. Méthodes et procédés d'évaluation non invasive de variations génétiques
WO2016040843A1 (fr) 2014-09-11 2016-03-17 Harry Stylli Procédés pour détecter le cancer de la prostate
EP3204512B1 (fr) 2014-10-10 2020-05-06 Sequenom, Inc. Méthodes pour la partitionnement des séquences génomiques
US11242559B2 (en) 2015-01-13 2022-02-08 The Chinese University Of Hong Kong Method of nuclear DNA and mitochondrial DNA analysis
US10364467B2 (en) 2015-01-13 2019-07-30 The Chinese University Of Hong Kong Using size and number aberrations in plasma DNA for detecting cancer
EP4428863A2 (fr) 2015-05-11 2024-09-11 Natera, Inc. Procédés et compositions pour déterminer la ploïdie
US11130986B2 (en) 2015-05-20 2021-09-28 Quantum-Si Incorporated Method for isolating target nucleic acid using heteroduplex binding proteins
WO2016189388A1 (fr) 2015-05-22 2016-12-01 Nipd Genetics Ltd Analyse parallèle multiplexée de régions génomiques ciblées pour des tests prénataux non invasifs
DK3168309T3 (da) 2015-11-10 2020-06-22 Eurofins Lifecodexx Gmbh Detektion af føtale kromosomale aneuploidier under anvendelse af dna-regioner med forskellig metylering mellem fosteret og det gravide hunkøn
WO2017205826A1 (fr) 2016-05-27 2017-11-30 Sequenom, Inc. Méthodes de détection de variations génétiques
JP7048105B2 (ja) 2016-07-15 2022-04-05 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 核酸ライブラリを生成する方法
WO2018022906A1 (fr) 2016-07-27 2018-02-01 Sequenom, Inc. Procédés d'évaluation non invasive de l'instabilité génomique
US11200963B2 (en) 2016-07-27 2021-12-14 Sequenom, Inc. Genetic copy number alteration classifications
CA3037366A1 (fr) 2016-09-29 2018-04-05 Myriad Women's Health, Inc. Depistage prenatal non invasif utilisant une optimisation de profondeur iterative dynamique
US11485996B2 (en) 2016-10-04 2022-11-01 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
CA3049457C (fr) 2017-01-20 2023-05-16 Sequenom, Inc. Procedes d'evaluation non invasive de variations du nombre de copies
US11929145B2 (en) 2017-01-20 2024-03-12 Sequenom, Inc Methods for non-invasive assessment of genetic alterations
CA3049455C (fr) 2017-01-20 2023-06-13 Sequenom, Inc. Fabrication et utilisation d'adaptateur de sequencage
JP7237003B2 (ja) 2017-01-24 2023-03-10 セクエノム， インコーポレイテッド 遺伝子片の評価のための方法およびプロセス
EP3998350A1 (fr) 2017-03-17 2022-05-18 Sequenom, Inc. Procédés et techniques d'évaluation d'un mosaïcisme génétique
US12084720B2 (en) 2017-12-14 2024-09-10 Natera, Inc. Assessing graft suitability for transplantation
US11584929B2 (en) 2018-01-12 2023-02-21 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
CA3090426A1 (fr) 2018-04-14 2019-10-17 Natera, Inc. Procedes de detection et de surveillance du cancer au moyen d'une detection personnalisee d'adn tumoral circulant
JP7537748B2 (ja) 2018-06-06 2024-08-21 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 核酸ライブラリを生成する方法ならびにそれを実施するための組成物およびキット
US11525159B2 (en) 2018-07-03 2022-12-13 Natera, Inc. Methods for detection of donor-derived cell-free DNA
SG11202110468YA (en) 2019-04-05 2021-10-28 Claret Bioscience Llc Methods and compositions for analyzing nucleic acid
CN110734966B (zh) * 2019-09-19 2021-06-25 广州医科大学附属肿瘤医院 一种用于检测sav1基因启动子区甲基化位点的检测体系及试剂盒
WO2021072037A1 (fr) 2019-10-09 2021-04-15 Claret Bioscience, Llc Procédés et compositions d'analyse d'acide nucléique
KR20220101108A (ko) 2019-10-29 2022-07-19 퀀텀-에스아이 인코포레이티드 유체의 연동 펌핑 및 연관 방법, 시스템, 및 디바이스
EP4052259A1 (fr) 2019-10-31 2022-09-07 Sequenom, Inc. Application d'un rapport du mosaïcisme dans des gestations multif?tales et évaluation de risque personnalisé
CN116096914A (zh) 2020-06-24 2023-05-09 克拉雷特生物科学有限责任公司 用于分析核酸的方法和组合物
WO2022076574A1 (fr) 2020-10-08 2022-04-14 Claret Bioscience, Llc Procédés et compositions d'analyse d'acide nucléique
CA3209074A1 (fr) * 2021-03-09 2022-09-15 Andrew Kennedy Preparation de bibliotheque genomique et dosages epigenetiques cibles utilisant des ribonucleoproteines cas-grna
WO2024186778A1 (fr) 2023-03-03 2024-09-12 Laboratory Corporation Of America Holdings Procédés et systèmes de criblage d'adncf positif sur des variations génétiques à l'aide d'un rapport de mosaïcisme

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6017704A (en) * 1996-06-03 2000-01-25 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
GB9704444D0 (en) 1997-03-04 1997-04-23 Isis Innovation Non-invasive prenatal diagnosis
US6251594B1 (en) * 1997-06-09 2001-06-26 Usc/Norris Comprehensive Cancer Ctr. Cancer diagnostic method based upon DNA methylation differences
DE19754482A1 (de) * 1997-11-27 1999-07-01 Epigenomics Gmbh Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke
US6331393B1 (en) * 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
JP2004505612A (ja) * 2000-03-31 2004-02-26 ユニバーシティ・オブ・サザン・カリフォルニア 食道腺ガンに関する後成的配列
US6927028B2 (en) * 2001-08-31 2005-08-09 Chinese University Of Hong Kong Non-invasive methods for detecting non-host DNA in a host using epigenetic differences between the host and non-host DNA
US7709194B2 (en) * 2004-06-04 2010-05-04 The Chinese University Of Hong Kong Marker for prenatal diagnosis and monitoring
US7901884B2 (en) * 2006-05-03 2011-03-08 The Chinese University Of Hong Kong Markers for prenatal diagnosis and monitoring

Also Published As

Publication number Publication date
CA3012120C (fr) 2021-08-10
US20150232949A1 (en) 2015-08-20
CA2887218C (fr) 2017-08-22
CA3012120A1 (fr) 2003-03-13
PT1421211E (pt) 2007-03-30
HK1076840A1 (en) 2006-01-27
CN1665936A (zh) 2005-09-07
DE60218182T2 (de) 2008-02-07
US6927028B2 (en) 2005-08-09
DK1421211T3 (da) 2007-05-29
CA2973165C (fr) 2018-08-28
EP1421211A2 (fr) 2004-05-26
ATE353977T1 (de) 2007-03-15
US8431343B2 (en) 2013-04-30
US20090170102A1 (en) 2009-07-02
ES2280562T3 (es) 2007-09-16
CA2456140A1 (fr) 2003-03-13
CN100352939C (zh) 2007-12-05
DE60218182D1 (de) 2007-03-29
US20050282185A1 (en) 2005-12-22
US20030044388A1 (en) 2003-03-06
CA2887218A1 (fr) 2003-03-13
US20200208225A1 (en) 2020-07-02
WO2003020974A2 (fr) 2003-03-13
US8168382B2 (en) 2012-05-01
AU2002324135A1 (en) 2003-03-18
US20130337443A1 (en) 2013-12-19
US8962280B2 (en) 2015-02-24
WO2003020974A3 (fr) 2003-09-18
EP1421211B1 (fr) 2007-02-14
US20120190029A1 (en) 2012-07-26
JP2005501566A (ja) 2005-01-20
CA2973165A1 (fr) 2003-03-13

Similar Documents

Publication Publication Date Title
US20200208225A1 (en) Systems for detecting dna orginating from different individuals
JP6634105B2 (ja) 非侵襲性の出生前診断のために有用な、母体サンプル由来の胎児核酸のメチル化に基づく濃縮のためのプロセスおよび組成物
JP6513622B2 (ja) 非侵襲的出生前診断に有用な母体試料由来の胎児核酸のメチル化に基づく富化のためのプロセスおよび組成物
US9719140B2 (en) Direct molecular diagnosis of fetal aneuploidy
US8394582B2 (en) Identification of fetal DNA and fetal cell markers in maternal plasma or serum
US20160258018A1 (en) Marker for prenatal diagnosis and monitoring
JP6302048B2 (ja) 次世代システムを用いるｈｌａ遺伝子アンプリコンのディープシーケンシングにより混合物を定量解析することによる固形臓器移植片拒絶の非侵襲的早期検出
GB2488358A (en) Enrichment of foetal DNA in maternal plasma
Tungwiwat et al. Non-invasive fetal sex determination using a conventional nested PCR analysis of fetal DNA in maternal plasma
EP2821503B1 (fr) Procédé de détection de l'allèle hla-a*31:01
JP2016516449A (ja) Ｈｌａマーカーを使用する母体血液中の胎児ｄｎａ分率の決定方法
KR101546364B1 (ko) 태아 특이적 후성학적 메틸화 마커 검출용 조성물 및 검출방법
KR101696707B1 (ko) 태아 특이적 후성학적 메틸화 마커 검출용 조성물 및 검출방법
KR101546366B1 (ko) 태아 특이적 후성학적 메틸화 마커 검출용 조성물 및 검출방법
van den Oever Noninvasive prenatal detection of genetic defects.
US20120100537A1 (en) Method of Prenatal Molecular Diagnosis of Down Syndrome and Other Trisomic Disorders

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20220830