CA2396795A1 - Fluorescent quenching detection reagents and methods - Google Patents

Fluorescent quenching detection reagents and methods Download PDF

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Publication number
CA2396795A1
CA2396795A1 CA002396795A CA2396795A CA2396795A1 CA 2396795 A1 CA2396795 A1 CA 2396795A1 CA 002396795 A CA002396795 A CA 002396795A CA 2396795 A CA2396795 A CA 2396795A CA 2396795 A1 CA2396795 A1 CA 2396795A1
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CA
Canada
Prior art keywords
moiety
linker
odn
accordance
fluorophore
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002396795A
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English (en)
French (fr)
Inventor
Michael W. Reed
Eugeny Alexander Lukhtanov
Alexander A. Gall
Robert O. Dempcy
Yevgeniy S. Belousov
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanogen Inc
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Individual
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Filing date
Publication date
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Publication of CA2396795A1 publication Critical patent/CA2396795A1/en
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D519/00—Heterocyclic compounds containing more than one system of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring system not provided for in groups C07D453/00 or C07D455/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C245/00—Compounds containing chains of at least two nitrogen atoms with at least one nitrogen-to-nitrogen multiple bond
    • C07C245/02—Azo compounds, i.e. compounds having the free valencies of —N=N— groups attached to different atoms, e.g. diazohydroxides
    • C07C245/06—Azo compounds, i.e. compounds having the free valencies of —N=N— groups attached to different atoms, e.g. diazohydroxides with nitrogen atoms of azo groups bound to carbon atoms of six-membered aromatic rings
    • C07C245/08—Azo compounds, i.e. compounds having the free valencies of —N=N— groups attached to different atoms, e.g. diazohydroxides with nitrogen atoms of azo groups bound to carbon atoms of six-membered aromatic rings with the two nitrogen atoms of azo groups bound to carbon atoms of six-membered aromatic rings, e.g. azobenzene
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
    • C07F9/02—Phosphorus compounds
    • C07F9/06—Phosphorus compounds without P—C bonds
    • C07F9/22—Amides of acids of phosphorus
    • C07F9/24—Esteramides
    • C07F9/2404—Esteramides the ester moiety containing a substituent or a structure which is considered as characteristic
    • C07F9/2408—Esteramides the ester moiety containing a substituent or a structure which is considered as characteristic of hydroxyalkyl compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/553—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having one nitrogen atom as the only ring hetero atom
    • C07F9/572—Five-membered rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/655—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms
    • C07F9/6552—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms the oxygen atom being part of a six-membered ring
    • C07F9/65522—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms the oxygen atom being part of a six-membered ring condensed with carbocyclic rings or carbocyclic ring systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/6561—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing systems of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring or ring system, with or without other non-condensed hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B29/00—Monoazo dyes prepared by diazotising and coupling
    • C09B29/06—Monoazo dyes prepared by diazotising and coupling from coupling components containing amino as the only directing group
    • C09B29/08—Amino benzenes
    • C09B29/0805—Amino benzenes free of acid groups
    • C09B29/0807—Amino benzenes free of acid groups characterised by the amino group
    • C09B29/0809—Amino benzenes free of acid groups characterised by the amino group substituted amino group
    • C09B29/0811—Amino benzenes free of acid groups characterised by the amino group substituted amino group further substituted alkylamino, alkenylamino, alkynylamino, cycloalkylamino aralkylamino or arylamino
    • C09B29/0813—Amino benzenes free of acid groups characterised by the amino group substituted amino group further substituted alkylamino, alkenylamino, alkynylamino, cycloalkylamino aralkylamino or arylamino substituted by OH, O-C(=X)-R, O-C(=X)-X-R, O-R (X being O,S,NR; R being hydrocarbonyl)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
CA002396795A 1999-12-08 2000-12-08 Fluorescent quenching detection reagents and methods Abandoned CA2396795A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/457,616 US6727356B1 (en) 1999-12-08 1999-12-08 Fluorescent quenching detection reagents and methods
US09/457,616 1999-12-08
PCT/US2000/033333 WO2001042505A2 (en) 1999-12-08 2000-12-08 Fluorescent quenching detection reagents and methods

Publications (1)

Publication Number Publication Date
CA2396795A1 true CA2396795A1 (en) 2001-06-14

Family

ID=23817445

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002396795A Abandoned CA2396795A1 (en) 1999-12-08 2000-12-08 Fluorescent quenching detection reagents and methods

Country Status (8)

Country Link
US (5) US6727356B1 (US06727356-20040427-C00006.png)
EP (1) EP1235938B1 (US06727356-20040427-C00006.png)
JP (2) JP5019688B2 (US06727356-20040427-C00006.png)
AT (1) ATE544771T1 (US06727356-20040427-C00006.png)
AU (1) AU782204B2 (US06727356-20040427-C00006.png)
CA (1) CA2396795A1 (US06727356-20040427-C00006.png)
ES (1) ES2381861T3 (US06727356-20040427-C00006.png)
WO (1) WO2001042505A2 (US06727356-20040427-C00006.png)

Families Citing this family (152)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7715989B2 (en) * 1998-04-03 2010-05-11 Elitech Holding B.V. Systems and methods for predicting oligonucleotide melting temperature (TmS)
US20040081959A9 (en) * 1999-12-08 2004-04-29 Epoch Biosciences, Inc. Fluorescent quenching detection reagents and methods
US6727356B1 (en) * 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
WO2001064958A2 (en) 2000-03-01 2001-09-07 Epoch Bioscienecs, Inc. Modified oligonucleotides for mismatch discrimination
EP1944310A3 (en) 2000-03-01 2008-08-06 Epoch Biosciences, Inc. Modified oligonucleotides for mismatch discrimination
US7019129B1 (en) * 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
DE60140804D1 (de) * 2000-06-27 2010-01-28 Nat Inst Of Advanced Ind Scien Neue nukleinsäuresonden und verfahren zum testen von nukleinsäuren unter deren verwendung
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
ATE518913T1 (de) * 2000-09-06 2011-08-15 Evotec Ag Verfahren zur gezielten markierung mittels affinitätsmarkierter detektionsmarker
US7267945B2 (en) * 2001-03-26 2007-09-11 Applera Corporation Methods of determining the presence of polynucleotides employing amplification
US20030082547A1 (en) 2001-08-27 2003-05-01 Ewing Gregory J. Non-fluorescent quencher compounds and biomolecular assays
WO2003043402A2 (en) * 2001-10-19 2003-05-30 Proligo Llc Nucleic acid probes and methods to detect and/or quantify nucleic acid analytes
JP2005516300A (ja) * 2002-01-25 2005-06-02 アプレラ コーポレイション 製品およびサービスに対する注文を発注し、受理し、および充足する方法
US20040072195A1 (en) * 2002-01-25 2004-04-15 Hunkapiller Michael W. Single-tube, ready-to-use assay kits, and methods using same
US20090239233A1 (en) * 2002-01-25 2009-09-24 Applera Corporation Single-tube, ready-to-use assay kits, and methods using same
US6818420B2 (en) 2002-02-27 2004-11-16 Biosource International, Inc. Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same
EP1485056B1 (en) * 2002-02-27 2011-04-06 Life Technologies Corporation Methods of using fet labeled oligonucleotides that include a'5' exonuclease resistant quencher domain and compositions for practicing the same
WO2003078450A2 (en) * 2002-03-11 2003-09-25 Epoch Biosciences, Inc. Negatively charged minor groove binders
US7176002B2 (en) * 2002-05-16 2007-02-13 Applera Corporation Universal-tagged oligonucleotide primers and methods of use
US7109316B2 (en) * 2002-08-23 2006-09-19 Ce Healthcare Bio-Sciences Corp. Oligonucleotide tagged nucleoside triphosphates (OTNTPs) for genetic analysis
AU2003275018B2 (en) * 2002-09-20 2009-10-01 Integrated Dna Technologies, Inc. Anthraquinone quencher dyes, their methods of preparation and use
EP1405920A3 (en) * 2002-10-02 2004-04-14 Roche Diagnostics GmbH Improved FRET process
CN100439515C (zh) * 2003-03-03 2008-12-03 清华大学 一种核酸分析芯片实验室系统与应用
CN1280428C (zh) * 2003-05-19 2006-10-18 清华大学 一种基于微小颗粒的生物芯片系统及其应用
US20070269803A1 (en) * 2003-06-26 2007-11-22 Khalil Arar Fluorogenic Nucleic Acid Probes Including Lna for Methods to Detect and/or Quantify Nucleic Acid Analytes
ES2529256T3 (es) * 2003-10-28 2015-02-18 Epoch Biosciences, Inc. Sondas fluorescentes para la detección de ADN mediante hibridación con una sensibilidad mejorada y baja señal de fondo
US20050112671A1 (en) * 2003-11-07 2005-05-26 U.S. Genomics, Inc. FRET efficiency methods
WO2005049849A2 (en) 2003-11-14 2005-06-02 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use
EP1697534B1 (en) 2003-12-01 2010-06-02 Life Technologies Corporation Nucleic acid molecules containing recombination sites and methods of using the same
WO2005118877A2 (en) 2004-06-02 2005-12-15 Vicus Bioscience, Llc Producing, cataloging and classifying sequence tags
US7635598B2 (en) 2004-07-08 2009-12-22 Biosearch Technologies, Inc. Inducible fluorescence assay
US7319022B1 (en) * 2004-08-13 2008-01-15 Epoch Biosciences, Inc. Amplification methods
US8183052B2 (en) * 2004-08-19 2012-05-22 Blood Cell Storage, Inc. Methods and apparatus for sterility testing
US8497134B2 (en) 2004-08-19 2013-07-30 Blood Cell Storage, Inc. Fluorescent detector systems for the detection of chemical perturbations in sterile storage devices
EP1627926A1 (en) * 2004-08-19 2006-02-22 Roche Diagnostics GmbH A method to reduce false positive results
JP5017113B2 (ja) 2004-08-19 2012-09-05 ブラッド セル ストレージ， インコーポレイテッド 蛍光ｐＨ検出システムおよび関連する方法
US8586718B2 (en) * 2004-09-14 2013-11-19 Applied Biosystems, Llc Multi-chromophoric quencher constructs for use in high sensitivity energy transfer probes
US7879986B2 (en) * 2005-02-04 2011-02-01 Berry & Associates, Inc. Dark quenchers, probes and other conjugates incorporating the same, and their use
AU2006223384B2 (en) * 2005-03-09 2012-08-02 Cepheid Polar dyes
CA2607530C (en) 2005-05-02 2014-07-08 Basf Aktiengesellschaft New labelling strategies for the sensitive detection of analytes
KR101335218B1 (ko) 2005-05-02 2013-12-12 바스프 에스이 분석물의 감응성 검출을 위한 신규한 표지화 전략
WO2006122002A2 (en) * 2005-05-09 2006-11-16 Panomics, Inc. Multiplex capture of nucleic acids
US8632970B2 (en) * 2005-05-09 2014-01-21 Affymetrix, Inc. Multiplex capture of nucleic acids
US7803541B2 (en) 2005-05-12 2010-09-28 Panomics, Inc. Multiplex branched-chain DNA assays
JP2008545659A (ja) 2005-05-20 2008-12-18 インテグレイテッド ディーエヌエイ テクノロジーズ インコーポレイテッド オリゴヌクレオチドを標識する化合物及び方法
GB0510979D0 (en) * 2005-05-28 2005-07-06 Kbiosciences Ltd Detection system for PCR assay
DK2500439T4 (da) * 2005-06-20 2017-11-13 Advanced Cell Diagnostics Inc Kits og produkter til detektering af nukleinsyrer i individuelle celler og til identifikation af sjældne celler fra store heterogene cellepopulationer
DE102005029811B4 (de) * 2005-06-27 2009-03-12 Siemens Ag Oligonukleotidanordnungen, Verfahren zu deren Einsatz und deren Verwendung
WO2007044427A2 (en) * 2005-10-05 2007-04-19 Panomics, Inc. Detection of nucleic acids from whole blood
EP1962833A4 (en) * 2005-11-15 2012-02-01 Gen Hospital Corp Light-Activatable Antimicrobial Agent
US7345303B2 (en) * 2005-12-22 2008-03-18 Xerox Corporation Organic thin-film transistors
WO2007106907A2 (en) * 2006-03-15 2007-09-20 Integrated Dna Technologies, Inc. Nucleic acid monomers with 2'-chemical moieties
WO2008002920A2 (en) * 2006-06-26 2008-01-03 Epoch Biosciences, Inc. Methods for generating target nucleic acid sequences
PT2089343E (pt) 2006-10-31 2011-09-06 Baseclick Gmbh Química click para a produção de moléculas repórter
US9938641B2 (en) 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
US7960139B2 (en) 2007-03-23 2011-06-14 Academia Sinica Alkynyl sugar analogs for the labeling and visualization of glycoconjugates in cells
JP5480130B2 (ja) * 2007-05-18 2014-04-23 サーモ フィッシャー サイエンティフィック バイオサイエンシィズ インク． 新規発色性シリル保護基およびオリゴヌクレオチド化学合成における新規発色性シリル保護基の利用
US8084589B2 (en) * 2007-08-31 2011-12-27 University Of Massachusetts Phosphoramidite nucleoside analogs
JP4557014B2 (ja) * 2008-02-14 2010-10-06 ソニー株式会社 核酸検出用蛍光標識オリゴヌクレオチド、及び該核酸検出用蛍光標識オリゴヌクレオチドを用いた二本鎖形成に関わる情報を得る方法
EP2268608A4 (en) * 2008-04-01 2012-01-11 Biosearch Technologies Inc DARK NUCLEIC ACID STABILIZED FLUOROPHORE DEACTIVATOR PROBES
EP2285381B1 (en) * 2008-05-05 2015-12-16 The General Hospital Corporation Photoactivatable beta-lactamase substrates and analytical methods of using same
US8097412B2 (en) * 2008-07-12 2012-01-17 Biodiagnostics, Inc. DNA-based test for detection of annual and intermediate ryegrass
DK2318832T3 (da) 2008-07-15 2014-01-20 Academia Sinica Glycan-arrays på PTFE-lignende aluminiumcoatede objektglas og relaterede fremgangsmåder
US9657338B2 (en) 2008-10-01 2017-05-23 Koninklijke Philips N.V. Method for immobilizing nucleic acids on a support
JP2012504412A (ja) * 2008-10-01 2012-02-23 コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ 支持体上の核酸の検査及び品質管理方法
US9534255B2 (en) 2008-12-17 2017-01-03 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
JP5805064B2 (ja) 2009-03-27 2015-11-04 ライフ テクノロジーズ コーポレーション 対立遺伝子変種を検出するための方法、組成物、およびキット
SG10201408401RA (en) 2009-09-16 2015-01-29 Genentech Inc Coiled coil and/or tether containing protein complexes and uses thereof
US11377485B2 (en) 2009-12-02 2022-07-05 Academia Sinica Methods for modifying human antibodies by glycan engineering
US10087236B2 (en) 2009-12-02 2018-10-02 Academia Sinica Methods for modifying human antibodies by glycan engineering
US20110151457A1 (en) 2009-12-22 2011-06-23 Elitech Holding B.V. Hypertheromostable endonuclease iv substrate probe
US9506057B2 (en) 2010-03-26 2016-11-29 Integrated Dna Technologies, Inc. Modifications for antisense compounds
AU2011230496B2 (en) 2010-03-26 2015-09-17 Integrated Dna Technologies, Inc. Methods for enhancing nucleic acid hybridization
AR080793A1 (es) 2010-03-26 2012-05-09 Roche Glycart Ag Anticuerpos biespecificos
US10338069B2 (en) 2010-04-12 2019-07-02 Academia Sinica Glycan arrays for high throughput screening of viruses
WO2011163120A1 (en) 2010-06-21 2011-12-29 Life Technologies Corporation Compositions, methods and kits for nucleic acid synthesis and amplification
US9410194B2 (en) 2010-06-21 2016-08-09 Life Technologies Corporation Compositions, kits and methods for synthesis and/or detection of nucleic acids
JP6053684B2 (ja) 2010-09-07 2016-12-27 インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド アンチセンス化合物の修飾
EP2630260B1 (en) 2010-10-21 2015-11-18 Advanced Cell Diagnostics, Inc. An ultra sensitive method for in situ detection of nucleic acids
KR20120042100A (ko) * 2010-10-22 2012-05-03 주식회사 씨젠 이중표지 고정화 프로브를 이용한 고상에서의 타겟 핵산서열 검출
EP2655413B1 (en) 2010-12-23 2019-01-16 F.Hoffmann-La Roche Ag Polypeptide-polynucleotide-complex and its use in targeted effector moiety delivery
ES2605493T3 (es) 2010-12-23 2017-03-14 F. Hoffmann-La Roche Ag Detección de un polipéptido modificado post-traduccionalmente mediante un agente ligante bivalente
US8969003B2 (en) 2011-03-23 2015-03-03 Elitech Holding B.V. Functionalized 3-alkynyl pyrazolopyrimidine analogues as universal bases and methods of use
WO2012129547A1 (en) 2011-03-23 2012-09-27 Elitech Holding B.V. Functionalized 3-alkynyl pyrazolopyrimidine analogues as universal bases and methods of use
ES2537189T3 (es) 2011-05-24 2015-06-03 Elitech Holding B.V. Detección de estafilococos áureos resistentes a la meticilina
US9040307B2 (en) 2011-05-27 2015-05-26 Blood Cell Storage, Inc. Fluorescent pH detector system and related methods
EP2744912B1 (en) 2011-08-18 2016-11-02 Nestec S.A. Compositions and methods for detecting allelic variants
CN104105711B (zh) 2012-02-10 2018-11-30 弗·哈夫曼-拉罗切有限公司 单链抗体及其他异多聚体
CN104379763B (zh) 2012-03-22 2017-04-19 Lgc基因组学有限公司 使用包含硫代磷酸酯基的寡核苷酸的聚合酶链式反应检测系统
US10130714B2 (en) 2012-04-14 2018-11-20 Academia Sinica Enhanced anti-influenza agents conjugated with anti-inflammatory activity
US20160073634A1 (en) 2012-06-22 2016-03-17 The General Hospital Corporation Beta-lactamase targeted photosensitizer for pesticide and pest detection
CN104411718B (zh) 2012-06-27 2018-04-24 弗·哈夫曼-拉罗切有限公司 用于制备包含特异性结合靶的至少一种结合实体的抗体Fc区缀合物的方法及其用途
MX354862B (es) 2012-06-27 2018-03-23 Hoffmann La Roche Método para la producción de entidades dirigidas altamente selectivas hechas a la medida y biespecíficas que contienen dos entidades de unión diferentes.
WO2014031498A1 (en) 2012-08-18 2014-02-27 Academia Sinica Cell-permeable probes for identification and imaging of sialidases
GB2515990B (en) 2013-03-06 2016-05-04 Lgc Genomics Ltd Polymerase chain reaction detection system
WO2014164479A1 (en) 2013-03-11 2014-10-09 Elitech Holding B.V. Methods for true isothermal strand displacement amplification
US10975423B2 (en) 2013-03-11 2021-04-13 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
WO2014164874A2 (en) 2013-03-13 2014-10-09 Becton, Dickinson And Company Methods and compositions for modulation of amplification efficiency
WO2014159063A1 (en) 2013-03-14 2014-10-02 Elitech Holding B.V. Functionalized 3-alkynyl pyrazolopyrimidine analogues as universal bases and methods of use
WO2014171899A1 (en) * 2013-04-19 2014-10-23 Agency For Science, Technology And Research Tunable fluorescence using cleavable linkers
WO2014186147A2 (en) 2013-05-13 2014-11-20 Elitech Holding B.V. Droplet digital pcr with short minor groove probes
WO2014210397A1 (en) 2013-06-26 2014-12-31 Academia Sinica Rm2 antigens and use thereof
EP3013347B1 (en) 2013-06-27 2019-12-11 Academia Sinica Glycan conjugates and use thereof
JP6486368B2 (ja) 2013-09-06 2019-03-20 アカデミア シニカＡｃａｄｅｍｉａ Ｓｉｎｉｃａ 改変されたグリコシル基を含む糖脂質を用いたヒトｉＮＫＴ細胞の活性化
US10150818B2 (en) 2014-01-16 2018-12-11 Academia Sinica Compositions and methods for treatment and detection of cancers
CA2937123A1 (en) 2014-01-16 2015-07-23 Academia Sinica Compositions and methods for treatment and detection of cancers
WO2015148915A1 (en) 2014-03-27 2015-10-01 Academia Sinica Reactive labelling compounds and uses thereof
EP2942401A1 (en) 2014-05-09 2015-11-11 Lifecodexx AG Detection of DNA that originates from a specific cell-type
EP2942400A1 (en) 2014-05-09 2015-11-11 Lifecodexx AG Multiplex detection of DNA that originates from a specific cell-type
RU2016147914A (ru) 2014-05-09 2018-06-18 Лайфкодекс Аг Обнаружение днк, которая происходит из специфического клеточного типа, и относящиеся к нему способы
JP2017518989A (ja) 2014-05-27 2017-07-13 アカデミア シニカＡｃａｄｅｍｉａ Ｓｉｎｉｃａ 抗ｃｄ２０糖操作抗体群およびその使用
US10005847B2 (en) 2014-05-27 2018-06-26 Academia Sinica Anti-HER2 glycoantibodies and uses thereof
US10118969B2 (en) 2014-05-27 2018-11-06 Academia Sinica Compositions and methods relating to universal glycoforms for enhanced antibody efficacy
JP6894239B2 (ja) 2014-05-27 2021-06-30 アカデミア シニカＡｃａｄｅｍｉａ Ｓｉｎｉｃａ 増強された抗体の有効性のための普遍的グリコフォームに関する組成物および方法
US11332523B2 (en) 2014-05-28 2022-05-17 Academia Sinica Anti-TNF-alpha glycoantibodies and uses thereof
WO2016040369A2 (en) 2014-09-08 2016-03-17 Academia Sinica HUMAN iNKT CELL ACTIVATION USING GLYCOLIPIDS
JP6721590B2 (ja) 2014-12-03 2020-07-15 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 多重特異性抗体
US9988670B2 (en) 2014-12-12 2018-06-05 Elitechgroup B.V. Methods and compositions for detecting antibiotic resistant bacteria
US10266903B2 (en) 2014-12-12 2019-04-23 Elitechgroup, Inc. Methods and compositions for detecting antibiotic resistant bacteria
US9975965B2 (en) 2015-01-16 2018-05-22 Academia Sinica Compositions and methods for treatment and detection of cancers
US10495645B2 (en) 2015-01-16 2019-12-03 Academia Sinica Cancer markers and methods of use thereof
DK3247710T3 (en) * 2015-01-20 2022-11-14 Biosearch Tech Inc Coumarin-based compounds and related methods
US10342858B2 (en) 2015-01-24 2019-07-09 Academia Sinica Glycan conjugates and methods of use thereof
US9957393B2 (en) 2015-03-30 2018-05-01 Enzo Biochem, Inc. Monoazo dyes with cyclic amine as fluorescence quenchers
WO2017066211A1 (en) 2015-10-12 2017-04-20 Advanced Cell Diagnostics, Inc. In situ detection of nucleotide variants in high noise samples, and compositions and methods related thereto
HUE050491T2 (hu) 2015-11-10 2020-12-28 Eurofins Lifecodexx Gmbh Magzati kromoszomális aneuploidiák kimutatása olyan DNS régiókat alkalmazva, amelyek különbözõképpen vannak metilezve a magzat és a terhes nõstény között
WO2017123647A1 (en) 2016-01-15 2017-07-20 Quantapore, Inc. Optically-based nanopore analysis with reduced background
WO2017156192A1 (en) 2016-03-08 2017-09-14 Academia Sinica Methods for modular synthesis of n-glycans and arrays thereof
AU2017248219B2 (en) 2016-04-06 2023-09-07 Life Technologies Corporation Compositions, methods, and kits for synthesis and detection of nucleic acids
WO2017200790A1 (en) 2016-05-20 2017-11-23 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Real-time reverse transcriptase-polymerase chain reaction assay with modified probe for the diagnosis of rabies viruses and other lyssaviruses
US10337051B2 (en) 2016-06-16 2019-07-02 The Regents Of The University Of California Methods and compositions for detecting a target RNA
WO2018013999A1 (en) 2016-07-15 2018-01-18 Am Chemicals Llc Non-nucleosidic solid supports and phosphoramidite building blocks for oligonucleotide synthesis
CN109804087A (zh) 2016-08-19 2019-05-24 昆塔波尔公司 使用猝灭剂的基于光学的纳米孔测序
US10538592B2 (en) 2016-08-22 2020-01-21 Cho Pharma, Inc. Antibodies, binding fragments, and methods of use
JP6730525B2 (ja) 2016-11-21 2020-07-29 ナノストリング テクノロジーズ，インコーポレイティド 化学組成物とそれを利用する方法
WO2018162986A2 (en) 2017-03-09 2018-09-13 Elitechgroup B.V. Nitrodiarylethenes as fluorescence quenchers for nucleic acid probes
ES2909351T3 (es) 2017-08-17 2022-05-06 Elitechgroup Inc Quencheres estabilizadores de fluorescencia dúplex para sondas de ácidos nucleicos
GB201713251D0 (en) 2017-08-18 2017-10-04 Lgc Genomics Ltd Methods and kits for detection of nucleic acid molecules
CN112533998B (zh) 2018-05-07 2023-06-20 塞弗德公司 磺酰罗丹明亚磷酰胺染料
US11549139B2 (en) 2018-05-14 2023-01-10 Nanostring Technologies, Inc. Chemical compositions and methods of using same
US11155713B2 (en) 2018-05-29 2021-10-26 Elitechgroup, Inc. Carborhodamine compounds and methods of preparation thereof
EP3830301A1 (en) 2018-08-01 2021-06-09 Mammoth Biosciences, Inc. Programmable nuclease compositions and methods of use thereof
US20210324461A1 (en) 2018-08-16 2021-10-21 Life Technologies Corporation Reagents, mixtures, kits and methods for amplification of nucleic acids
EP3931313A2 (en) 2019-01-04 2022-01-05 Mammoth Biosciences, Inc. Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection
EP3935156A4 (en) 2019-03-07 2022-12-28 The Regents of The University of California CRISPR-CAS EFFECTOR POLYPEPTIDES AND METHODS OF USE
WO2021080629A1 (en) 2019-10-23 2021-04-29 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
WO2021240443A1 (en) 2020-05-27 2021-12-02 King Abdullah University Of Science And Technology Iscan: an rt-lamp-coupled crispr-cas module for rapid, sensitive detection of sars-cov-2
CA3185976A1 (en) 2020-07-16 2022-01-20 Kevin P. Lund Fluorescent dyes
EP4185596A1 (en) 2020-07-23 2023-05-31 Life Technologies Corporation Energy transfer dye conjugates for use in biological assays
EP4185858A2 (en) 2020-07-23 2023-05-31 Life Technologies Corporation Compositions, systems and methods for biological analysis using dyes
WO2023004400A1 (en) 2021-07-21 2023-01-26 Life Technologies Corporation Dibenzoxanthene quenchers, uses, and methods of preparation
WO2023023673A2 (en) 2021-08-20 2023-02-23 Kasa Bio, L.L.C. Compositions and methods for multiplex detection of mirna and other polynucelotides

Family Cites Families (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US952202A (en) * 1909-05-22 1910-03-15 Heinrich Klingenfeld Combined garment support and form.
US2264303A (en) 1940-03-27 1941-12-02 Eastman Kodak Co Azo compounds and material colored therewith
US3407189A (en) 1956-07-25 1968-10-22 Sandoz Ltd Water-insoluble monoazo dyestuffs
GB1394368A (en) 1972-12-14 1975-05-14 Ici Ltd Disperse azo dyestuffs
US3970617A (en) * 1974-03-07 1976-07-20 E. I. Du Pont De Nemours And Company Navy-blue nitrophenylazonaphthylamino dyes
GB1533121A (en) 1975-08-04 1978-11-22 Ici Ltd Disperse nitro disazo dyestuffs
US4358535A (en) 1980-12-08 1982-11-09 Board Of Regents Of The University Of Washington Specific DNA probes in diagnostic microbiology
US4711955A (en) 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
CA1190838A (en) 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
US4570542A (en) * 1982-05-17 1986-02-18 Weld Tooling Corporation Ribbon rail systems
DE3526565A1 (de) 1985-07-25 1987-02-05 Boehringer Mannheim Gmbh Resorufin-derivate, verfahren zu deren herstellung sowie deren verwendung in fluoreszenzimmunoassays
FR2588495B1 (fr) * 1985-10-16 1987-12-11 Aerospatiale Ensemble de douille de fixation pour unite d'usinage et unite d'usinage adaptee pour cooperer avec ladite douille
US4868105A (en) 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US5202231A (en) 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
US6270961B1 (en) 1987-04-01 2001-08-07 Hyseq, Inc. Methods and apparatus for DNA sequencing and DNA identification
US5585481A (en) 1987-09-21 1996-12-17 Gen-Probe Incorporated Linking reagents for nucleotide probes
US5124246A (en) 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5824796A (en) 1988-09-28 1998-10-20 Epoch Pharmaceuticals, Inc. Cross-linking oligonucleotides
US4850763A (en) * 1988-10-03 1989-07-25 The Boeing Company Tool track for use on aircraft
US5272259A (en) * 1988-12-23 1993-12-21 Bayer Aktiengesellschaft Stilbene dyestuffs and light-polarizing films or sheets containing stilbene
EP0472648A4 (en) 1989-05-18 1992-09-16 Microprobe Corporation Crosslinking oligonucleotides
DE3916595A1 (de) 1989-05-22 1990-11-29 Boehringer Mannheim Gmbh Verfahren zur nichtradioaktiven messung der nucleinsaeuresynthese in eukaryontischen zellen
US5451463A (en) 1989-08-28 1995-09-19 Clontech Laboratories, Inc. Non-nucleoside 1,3-diol reagents for labeling synthetic oligonucleotides
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5512667A (en) 1990-08-28 1996-04-30 Reed; Michael W. Trifunctional intermediates for preparing 3'-tailed oligonucleotides
DE69132905T2 (de) 1990-12-06 2002-08-01 Affymetrix Inc N D Ges D Staat Detektion von Nukleinsäuresequenzen
JPH04279556A (ja) * 1991-03-07 1992-10-05 Nippon Oil & Fats Co Ltd フルオロアルキル基含有アゾ化合物及びその製造方法
EP0528512A1 (en) * 1991-08-20 1993-02-24 Pioneer Electronic Corporation Recording medium
JPH0578300A (ja) * 1991-09-19 1993-03-30 Nippon Oil & Fats Co Ltd フルオロアルキル基含有アゾ化合物及びその製造方法
ES2159282T3 (es) 1991-11-07 2001-10-01 Nanotronics Inc Hibridacion de polinucleotidos conjugados con cromoforas y fluoroforos con el fin de generar sistemas de transferencia de energia de donador a donador.
TW393513B (en) 1991-11-26 2000-06-11 Isis Pharmaceuticals Inc Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines
US5502177A (en) * 1993-09-17 1996-03-26 Gilead Sciences, Inc. Pyrimidine derivatives for labeled binding partners
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5395187A (en) * 1993-11-22 1995-03-07 Mcdonnell Douglas Corporation Method and apparatus for attaching a drill motor to a drill plate with a clamping device having an expandable collet
JPH07179773A (ja) * 1993-12-01 1995-07-18 Dainichiseika Color & Chem Mfg Co Ltd アゾ化合物
US5556752A (en) 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA
US5482411A (en) * 1994-12-22 1996-01-09 Mcdonnell Douglas Corporation Method and apparatus for securely clamping a drill motor to a drill plate
US5801155A (en) 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
US6312894B1 (en) * 1995-04-03 2001-11-06 Epoch Pharmaceuticals, Inc. Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
US20010018514A1 (en) 1998-07-31 2001-08-30 Mcgall Glenn H. Nucleic acid labeling compounds
GB9602265D0 (en) 1996-02-05 1996-04-03 Amersham Int Plc Benzophenoxazine dyes
AU713667B2 (en) 1996-04-12 1999-12-09 Phri Properties, Inc. Detection probes, kits and assays
US6036409A (en) * 1997-01-23 2000-03-14 The Boeing Company Blind hole self-collet countersink
US5846726A (en) 1997-05-13 1998-12-08 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
AU9220498A (en) 1997-09-04 1999-03-22 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of usethereof
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
US6028183A (en) 1997-11-07 2000-02-22 Gilead Sciences, Inc. Pyrimidine derivatives and oligonucleotides containing same
US6007992A (en) 1997-11-10 1999-12-28 Gilead Sciences, Inc. Pyrimidine derivatives for labeled binding partners
US6210084B1 (en) * 1997-11-26 2001-04-03 The Boeing Company Pressure foot assembly for clamping a joint
ATE469982T1 (de) 1998-02-04 2010-06-15 Life Technologies Corp Bestimmung des genotyps eines amplifikationsproduktes an mehreren allelen stellen
US6220099B1 (en) * 1998-02-17 2001-04-24 Ce Nuclear Power Llc Apparatus and method for performing non-destructive inspections of large area aircraft structures
US6073328A (en) * 1998-02-28 2000-06-13 Hendricks; James Carpet installation tool
US5952202A (en) 1998-03-26 1999-09-14 The Perkin Elmer Corporation Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification
US6117986A (en) 1998-06-10 2000-09-12 Intergen Company, L.P. Pyrimidines linked to a quencher
US6240322B1 (en) * 1998-11-04 2001-05-29 Cardiac Pacemakers, Inc. System and apparatus having low profile collapsible tines
ATE233795T1 (de) 1999-04-23 2003-03-15 Molecular Probes Inc Xanthenfarbstoffe und ihre anwendung als lumineszenzlöschende verbindungen
US6151241A (en) 1999-05-19 2000-11-21 Symetrix Corporation Ferroelectric memory with disturb protection
US6114518A (en) 1999-09-30 2000-09-05 Becton, Dickinson And Company Synthesis and use of labelled phosphoramidite compositions
US6467385B1 (en) * 1999-12-03 2002-10-22 The Boeing Company Panel trimming system
US6727356B1 (en) * 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
US6465644B1 (en) 2000-05-02 2002-10-15 Applera Corporation Sulfonated [8,9] benzophenoxazine dyes and the use of their labelled conjugates
US6430796B1 (en) * 2000-05-03 2002-08-13 The Boeing Company Apparatus for performing automated manufacturing operations on panel-shaped workpieces
US6323337B1 (en) 2000-05-12 2001-11-27 Molecular Probes, Inc. Quenching oligonucleotides
US6550129B1 (en) * 2000-09-25 2003-04-22 The Boeing Company Portable computer numeric controlled manufacturing machines and associated methods
US6448407B1 (en) 2000-11-01 2002-09-10 Pe Corporation (Ny) Atropisomers of asymmetric xanthene fluorescent dyes and methods of DNA sequencing and fragment analysis

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