CA2377693C - Methods for producing l-amino acids - Google Patents
Methods for producing l-amino acids Download PDFInfo
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- CA2377693C CA2377693C CA2377693A CA2377693A CA2377693C CA 2377693 C CA2377693 C CA 2377693C CA 2377693 A CA2377693 A CA 2377693A CA 2377693 A CA2377693 A CA 2377693A CA 2377693 C CA2377693 C CA 2377693C
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- Prior art keywords
- corynebacterium glutamicum
- altered
- cell
- pgi
- gene
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000007858 starting material Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 125000001424 substituent group Chemical group 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 1
- 230000000153 supplemental effect Effects 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 229960004659 ticarcillin Drugs 0.000 description 1
- OHKOGUYZJXTSFX-KZFFXBSXSA-N ticarcillin Chemical compound C=1([C@@H](C(O)=O)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)C=CSC=1 OHKOGUYZJXTSFX-KZFFXBSXSA-N 0.000 description 1
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- JTSDBFGMPLKDCD-XVFHVFLVSA-N tilmicosin Chemical compound O([C@@H]1[C@@H](C)[C@H](O)CC(=O)O[C@@H]([C@H](/C=C(\C)/C=C/C(=O)[C@H](C)C[C@@H]1CCN1C[C@H](C)C[C@H](C)C1)CO[C@H]1[C@@H]([C@H](OC)[C@H](O)[C@@H](C)O1)OC)CC)[C@@H]1O[C@H](C)[C@@H](O)[C@H](N(C)C)[C@H]1O JTSDBFGMPLKDCD-XVFHVFLVSA-N 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
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- 230000009466 transformation Effects 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 150000003627 tricarboxylic acid derivatives Chemical class 0.000 description 1
- IEDVJHCEMCRBQM-UHFFFAOYSA-N trimethoprim Chemical compound COC1=C(OC)C(OC)=CC(CC=2C(=NC(N)=NC=2)N)=C1 IEDVJHCEMCRBQM-UHFFFAOYSA-N 0.000 description 1
- 229960001082 trimethoprim Drugs 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 1
- 229910052721 tungsten Inorganic materials 0.000 description 1
- 239000010937 tungsten Substances 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 230000003827 upregulation Effects 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- ZJSAHDQVNRJQGU-UHFFFAOYSA-N urea;nitrate Chemical compound NC(N)=O.[O-][N+]([O-])=O ZJSAHDQVNRJQGU-UHFFFAOYSA-N 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
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- 239000011715 vitamin B12 Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 108010027345 wheylin-1 peptide Proteins 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
- C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US14521799P | 1999-07-23 | 1999-07-23 | |
| US60/145,217 | 1999-07-23 | ||
| US15001799P | 1999-08-20 | 1999-08-20 | |
| US60/150,017 | 1999-08-20 | ||
| PCT/US2000/019914 WO2001007626A2 (en) | 1999-07-23 | 2000-07-21 | Methods for producing l-amino acids by increasing cellular nadph |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2377693A1 CA2377693A1 (en) | 2001-02-01 |
| CA2377693C true CA2377693C (en) | 2016-11-08 |
Family
ID=26842765
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2377693A Expired - Fee Related CA2377693C (en) | 1999-07-23 | 2000-07-21 | Methods for producing l-amino acids |
Country Status (16)
| Country | Link |
|---|---|
| US (2) | US6465238B1 (enExample) |
| EP (1) | EP1208205B1 (enExample) |
| JP (2) | JP4841093B2 (enExample) |
| KR (1) | KR100575403B1 (enExample) |
| CN (1) | CN1372597A (enExample) |
| AT (1) | ATE340857T1 (enExample) |
| AU (1) | AU6362200A (enExample) |
| BR (1) | BR0012712B1 (enExample) |
| CA (1) | CA2377693C (enExample) |
| DE (1) | DE60030988T2 (enExample) |
| DK (1) | DK1208205T3 (enExample) |
| ES (1) | ES2272303T3 (enExample) |
| MX (1) | MXPA02000729A (enExample) |
| NZ (1) | NZ517295A (enExample) |
| PT (1) | PT1208205E (enExample) |
| WO (1) | WO2001007626A2 (enExample) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PT1208205E (pt) * | 1999-07-23 | 2006-12-29 | Archer Daniels Midland Co | Métodos para produzir l-aminoácidos por aumento do nadph celular |
| US6830903B1 (en) | 1999-07-23 | 2004-12-14 | Archer-Daniels-Midland Company | Methods for producing L-amino acids using a corynebacterium glutamicum with a disrupted pgi gene |
| US6586214B1 (en) * | 1999-09-15 | 2003-07-01 | Degussa Ag | Method for increasing the metabolic flux through the pentose phosphate cycle in coryneform bacteria by regulation of the phosphoglucose isomerase (pgi gene) |
| KR100826815B1 (ko) * | 2000-03-20 | 2008-05-02 | 에보니크 데구사 게엠베하 | ｇｎｄ 유전자의 증폭을 이용한 Ｌ-아미노산의 발효적생산 방법 |
| DE10112992A1 (de) * | 2001-03-17 | 2002-09-26 | Degussa | Verfahren zur fermentativen Herstellung von L-Aminosäuren unter Verwendung coryneformer Bakterien |
| US7160711B2 (en) * | 2001-08-06 | 2007-01-09 | Degussa Ag | Coryneform bacteria which produce chemical compounds I |
| DE10220574A1 (de) * | 2002-05-08 | 2003-11-20 | Degussa | Verfahren zur Herstellung von Aminosäuren mit coryneformen Bakterien unter Verwendung von Phosphoglucose-Isomerasen aus coryneformen Bakterien |
| MXPA06006759A (es) | 2003-12-18 | 2006-09-04 | Basf Ag | Metodos para la preparacion de lisina por fermentacion de corynebacterium glutamicum. |
| WO2005059154A2 (en) * | 2003-12-18 | 2005-06-30 | Basf Aktiengesellschaft | Methods for the preparation of a fine chemical by fermentation |
| CA2546847A1 (en) * | 2003-12-18 | 2005-12-22 | Basf Aktiengesellschaft | Methods for the preparation of a fine chemical by fermentation |
| RU2288264C2 (ru) * | 2004-02-12 | 2006-11-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) | Бактерия, принадлежащая к роду escherichia - продуцент l-треонина и способ получения l-треонина |
| US20050191684A1 (en) * | 2004-02-25 | 2005-09-01 | Zimenkov Danila V. | Method for producing L-amino acids |
| RU2004105179A (ru) * | 2004-02-25 | 2005-08-10 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) | 6-фосфоглюконолактоназа из escherichia coli, фрагмент днк, бактерия, принадлежащая к роду escherichia, -продуцент l-аминокислоты, и способ получения l-аминокислоты |
| JP4670811B2 (ja) * | 2004-02-25 | 2011-04-13 | 味の素株式会社 | Ｌ−アミノ酸の生産方法 |
| EP1724344B1 (en) * | 2004-02-27 | 2011-02-23 | Kyowa Hakko Bio Co., Ltd. | Process for producing amino acid |
| KR100563226B1 (ko) * | 2004-08-10 | 2006-03-21 | 한국생산기술연구원 | 연료전지용 분리판 |
| RU2004124226A (ru) * | 2004-08-10 | 2006-01-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) | Использование фосфокетолазы для продукции полезных метаболитов |
| KR101357015B1 (ko) * | 2004-12-22 | 2014-02-03 | 더 미시간 바이오테크노로지 인스티튜트 | 유기산의 증가된 생성을 위한 재조합 미생물 |
| DE102005013676A1 (de) | 2005-03-24 | 2006-09-28 | Degussa Ag | Allele des zwf-Gens aus coryneformen Bakterien |
| DE102005023829A1 (de) | 2005-05-24 | 2006-11-30 | Degussa Ag | Allele des opcA-Gens aus coryneformen Bakterien |
| DE102005048818A1 (de) | 2005-10-10 | 2007-04-12 | Degussa Ag | Mikrobiologische Herstellung von 3-Hydroxypropionsäure |
| KR100873168B1 (ko) * | 2007-03-07 | 2008-12-10 | 전북대학교산학협력단 | ｌｏｎ 유전자 및／또는 ｃｐｘＲ 유전자가 결실된살모넬라 변이균주 및 이를 함유하는 살모넬라 생백신 |
| US7785861B2 (en) | 2007-11-10 | 2010-08-31 | Joule Unlimited, Inc. | Hyperphotosynthetic organisms |
| EP2553107A2 (en) * | 2010-03-31 | 2013-02-06 | E.I. Du Pont De Nemours And Company | Pentose phosphate pathway upregulation to increase production of non-native products of interest in transgenic microorganisms |
| US9181568B2 (en) | 2012-04-23 | 2015-11-10 | Exxonmobil Research And Engineering Company | Cell systems and methods for improving fatty acid synthesis by expression of dehydrogenases |
| KR101429814B1 (ko) * | 2012-10-05 | 2014-08-12 | 상지대학교산학협력단 | Ｇｄｈ 활성 조절을 통해 ｌ-쓰레오닌 생산능이 향상된 코리네박테리움 속 미생물 및 이를 이용한 ｌ-쓰레오닌 생산 방법 |
| US10689671B2 (en) | 2014-05-12 | 2020-06-23 | Metabolic Explorer | Microorganism and method for the production of 1.2-propanediol based on NADPH dependent acetol reductase and improved NADPH supply |
| KR102384227B1 (ko) | 2015-05-18 | 2022-04-07 | 삼성전자주식회사 | Nadph 생성이 증가된 유전적으로 조작된 효모 세포, 효모 세포 중 nadph 수준을 증가시키는 방법, 상기 효모 세포를 제조하는 방법 및 상기 효모 세포를 이용하여 락테이트를 생산하는 방법 |
| CN112725253B (zh) * | 2020-12-30 | 2023-01-06 | 宁夏伊品生物科技股份有限公司 | 一种改造基因bbd29_14900的重组菌株及其构建方法与应用 |
| CN113930465A (zh) * | 2021-10-21 | 2022-01-14 | 呼伦贝尔东北阜丰生物科技有限公司 | 一种苏氨酸发酵代谢调控工艺 |
| CN113774096A (zh) * | 2021-10-21 | 2021-12-10 | 呼伦贝尔东北阜丰生物科技有限公司 | 一种苏氨酸生产提取工艺的优化方法 |
| CN114181288B (zh) * | 2022-02-17 | 2022-05-03 | 北京中科伊品生物科技有限公司 | 制备l-缬氨酸的方法及其所用的基因与该基因编码的蛋白质 |
Family Cites Families (11)
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|---|---|---|---|---|
| JPS594988B2 (ja) * | 1976-02-03 | 1984-02-02 | 日石三菱株式会社 | グリコ−スイソメラ−ゼの製法 |
| EP0672161B1 (en) | 1992-11-05 | 1999-09-22 | Xyrofin Oy | Recombinant method and host for manufacture of xylitol |
| DE69326559T2 (de) | 1992-11-05 | 2000-02-10 | Xyrofin Oy, Espoo | Rekombinante methode und wirt zur herstellung von xylitol |
| US5830716A (en) | 1993-10-28 | 1998-11-03 | Ajinomoto Co., Inc. | Increased amounts of substances by modifying a microorganism to increase production of NADPH from NADH |
| EP0780477B1 (en) | 1994-08-19 | 2003-04-09 | Ajinomoto Co., Inc. | Process for producing l-lysine and l-glutamic acid by fermentation |
| JPH09224662A (ja) * | 1996-02-23 | 1997-09-02 | Mitsubishi Chem Corp | ６−ホスホグルコン酸デヒドロゲナーゼおよびそれをコードするｄｎａ |
| JPH09224661A (ja) * | 1996-02-23 | 1997-09-02 | Mitsubishi Chem Corp | グルコース−６−リン酸デヒドロゲナーゼおよびそれをコードするｄｎａ |
| ID25613A (id) * | 1997-07-18 | 2000-10-19 | Ajinomoto Kk | Metode untuk menghasilkan purin nukleosida melalui fermentasi |
| FR2772788B1 (fr) | 1997-12-19 | 2000-11-17 | Roquette Freres | Procede pour accroitre la production de certains polyols par une souche osmotolerante |
| PT1208205E (pt) * | 1999-07-23 | 2006-12-29 | Archer Daniels Midland Co | Métodos para produzir l-aminoácidos por aumento do nadph celular |
| US6586214B1 (en) * | 1999-09-15 | 2003-07-01 | Degussa Ag | Method for increasing the metabolic flux through the pentose phosphate cycle in coryneform bacteria by regulation of the phosphoglucose isomerase (pgi gene) |
-
2000
- 2000-07-21 PT PT00950529T patent/PT1208205E/pt unknown
- 2000-07-21 BR BRPI0012712-4A patent/BR0012712B1/pt not_active IP Right Cessation
- 2000-07-21 DE DE60030988T patent/DE60030988T2/de not_active Expired - Lifetime
- 2000-07-21 NZ NZ517295A patent/NZ517295A/en not_active IP Right Cessation
- 2000-07-21 KR KR1020027000925A patent/KR100575403B1/ko not_active Expired - Lifetime
- 2000-07-21 CA CA2377693A patent/CA2377693C/en not_active Expired - Fee Related
- 2000-07-21 JP JP2001512892A patent/JP4841093B2/ja not_active Expired - Lifetime
- 2000-07-21 WO PCT/US2000/019914 patent/WO2001007626A2/en not_active Ceased
- 2000-07-21 US US09/621,451 patent/US6465238B1/en not_active Expired - Lifetime
- 2000-07-21 AU AU63622/00A patent/AU6362200A/en not_active Abandoned
- 2000-07-21 CN CN00810748A patent/CN1372597A/zh active Pending
- 2000-07-21 EP EP00950529A patent/EP1208205B1/en not_active Expired - Lifetime
- 2000-07-21 DK DK00950529T patent/DK1208205T3/da active
- 2000-07-21 MX MXPA02000729A patent/MXPA02000729A/es active IP Right Grant
- 2000-07-21 AT AT00950529T patent/ATE340857T1/de not_active IP Right Cessation
- 2000-07-21 ES ES00950529T patent/ES2272303T3/es not_active Expired - Lifetime
-
2002
- 2002-08-20 US US10/223,355 patent/US6680190B2/en not_active Expired - Lifetime
-
2010
- 2010-12-01 JP JP2010268851A patent/JP2011041582A/ja not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| JP2003521888A (ja) | 2003-07-22 |
| DK1208205T3 (da) | 2007-02-05 |
| JP4841093B2 (ja) | 2011-12-21 |
| ATE340857T1 (de) | 2006-10-15 |
| WO2001007626A2 (en) | 2001-02-01 |
| EP1208205B1 (en) | 2006-09-27 |
| WO2001007626A3 (en) | 2001-05-31 |
| AU6362200A (en) | 2001-02-13 |
| JP2011041582A (ja) | 2011-03-03 |
| KR100575403B1 (ko) | 2006-05-03 |
| DE60030988T2 (de) | 2007-01-04 |
| BR0012712B1 (pt) | 2011-12-13 |
| DE60030988D1 (de) | 2006-11-09 |
| US6465238B1 (en) | 2002-10-15 |
| ES2272303T3 (es) | 2007-05-01 |
| KR20020048928A (ko) | 2002-06-24 |
| NZ517295A (en) | 2004-04-30 |
| BR0012712A (pt) | 2002-04-09 |
| CA2377693A1 (en) | 2001-02-01 |
| EP1208205A2 (en) | 2002-05-29 |
| US20030017557A1 (en) | 2003-01-23 |
| CN1372597A (zh) | 2002-10-02 |
| MXPA02000729A (es) | 2002-08-20 |
| US6680190B2 (en) | 2004-01-20 |
| PT1208205E (pt) | 2006-12-29 |
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