CA1341532C - Heterologous polypeptides expressed in filamentous fungi, processes for making same, and vectors for making same - Google Patents
Heterologous polypeptides expressed in filamentous fungi, processes for making same, and vectors for making same Download PDFInfo
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- CA1341532C CA1341532C CA000516993A CA516993A CA1341532C CA 1341532 C CA1341532 C CA 1341532C CA 000516993 A CA000516993 A CA 000516993A CA 516993 A CA516993 A CA 516993A CA 1341532 C CA1341532 C CA 1341532C
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- 229910052708 sodium Inorganic materials 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 235000010344 sodium nitrate Nutrition 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 229960002385 streptomycin sulfate Drugs 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
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- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 108091008023 transcriptional regulators Proteins 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6478—Aspartic endopeptidases (3.4.23)
- C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
- Peptides Or Proteins (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US77137485A | 1985-08-29 | 1985-08-29 | |
| US771,374 | 1985-08-29 | ||
| US88222486A | 1986-07-07 | 1986-07-07 | |
| US882,224 | 1986-07-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1341532C true CA1341532C (en) | 2007-06-19 |
Family
ID=27118452
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000516993A Expired - Fee Related CA1341532C (en) | 1985-08-29 | 1986-08-28 | Heterologous polypeptides expressed in filamentous fungi, processes for making same, and vectors for making same |
Country Status (10)
| Country | Link |
|---|---|
| EP (2) | EP0215594B2 (xx) |
| JP (1) | JPH08280388A (xx) |
| AT (1) | ATE117020T1 (xx) |
| AU (1) | AU607398B2 (xx) |
| CA (1) | CA1341532C (xx) |
| DE (1) | DE3650202T3 (xx) |
| DK (1) | DK175460B1 (xx) |
| HK (1) | HK1003579A1 (xx) |
| IE (2) | IE65794B1 (xx) |
| NZ (1) | NZ217373A (xx) |
Families Citing this family (127)
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|---|---|---|---|---|
| US4885249A (en) * | 1984-12-05 | 1989-12-05 | Allelix, Inc. | Aspergillus niger transformation system |
| GB8508800D0 (en) * | 1985-04-04 | 1985-05-09 | Glaxo Group Ltd | Microbiological process |
| US5198345A (en) * | 1985-04-15 | 1993-03-30 | Gist-Brocades N.V. | Vectors in use in filamentous fungi |
| DE3650783T2 (de) * | 1985-04-15 | 2004-07-15 | Dsm Ip Assets B.V. | Verwendung des Glucoamylasepromotors aus Apergillus |
| US5766912A (en) * | 1986-03-17 | 1998-06-16 | Novo Nordisk A/S | Humicola lipase produced in aspergillus |
| DK122686D0 (da) * | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| US5536661A (en) * | 1987-03-10 | 1996-07-16 | Novo Nordisk A/S | Process for the production of protein products in aspergillus |
| US5780292A (en) * | 1987-04-29 | 1998-07-14 | Alko Group Ltd. | Production of phytate degrading enzymes in trichoderma |
| GB8610600D0 (en) * | 1986-04-30 | 1986-06-04 | Novo Industri As | Transformation of trichoderma |
| US5610034A (en) * | 1987-04-29 | 1997-03-11 | Alko Group Ltd. | Immunoglobulin production by trichoderma |
| ATE125865T1 (de) * | 1987-08-28 | 1995-08-15 | Novo Nordisk As | Rekombinante humicola-lipase und verfahren zur herstellung von rekombinanten humicola-lipasen. |
| WO1989001969A1 (en) * | 1987-09-04 | 1989-03-09 | Novo-Nordisk A/S | Process for the production of protein products in aspergillus and promoters for use in aspergillus |
| CA1333777C (en) * | 1988-07-01 | 1995-01-03 | Randy M. Berka | Aspartic proteinase deficient filamentous fungi |
| EP0429628B1 (en) * | 1989-06-16 | 1998-09-02 | Genencor International, Inc. | Dna sequences, vectors, and fusion polypeptides to increase secretion of desired polypeptides from filamentous fungi |
| FR2649120B1 (fr) * | 1989-06-30 | 1994-01-28 | Cayla | Nouvelle souche et ses mutants de champignons filamenteux, procede de production de proteines recombinantes a l'aide de ladite souche et souches et proteines obtenues selon ce procede |
| WO1991013971A1 (en) * | 1990-03-13 | 1991-09-19 | Hawaii Biotechnology Group, Inc. | Neurospora expression system |
| WO1992001797A1 (en) * | 1990-07-16 | 1992-02-06 | Oy Alko Ab | IMMUNOGLOBULIN PRODUCTION BY $i(TRICHODERMA) |
| WO1992016634A1 (en) * | 1991-03-22 | 1992-10-01 | Novo Nordisk A/S | A process for producing heme proteins |
| ATE123524T1 (de) * | 1991-03-22 | 1995-06-15 | Novo Nordisk As | Herstellungsverfahren für chymosin. |
| DK36592D0 (da) * | 1992-03-20 | 1992-03-20 | Novo Nordisk As | Fremgangsmaade til fremstilling af proteiner |
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| ES2198410T3 (es) * | 1993-07-23 | 2004-02-01 | Dsm Ip Assets B.V. | Cepas recombinantes de hongos filamentosos libres de gen marcador de seleccion: un metodo para obtener dichas cepas y su utilizacion. |
| US6300114B1 (en) | 1994-07-29 | 2001-10-09 | Rohm Enzyme Finland Oy | Sequences of xylanase and xylanase expression vectors |
| US5935836A (en) * | 1994-07-29 | 1999-08-10 | Rohm Enzyme Finland Oy | Actinomadura xylanase sequences and methods of use |
| US7816129B2 (en) | 1994-07-29 | 2010-10-19 | Ab Enzymes Gmbh | Production and secretion of proteins of bacterial origin in filamentous fungi |
| US5700686A (en) * | 1995-06-06 | 1997-12-23 | Iogen Corporation | Protease-treated and purified cellulase compositions and methods for reducing backstaining during enzymatic stonewashing |
| US7883872B2 (en) | 1996-10-10 | 2011-02-08 | Dyadic International (Usa), Inc. | Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose |
| US6265204B1 (en) | 1997-01-17 | 2001-07-24 | Genencor International, Inc. | DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi |
| DE69922978T2 (de) * | 1998-10-06 | 2005-12-08 | Emalfarb, Mark Aaron, Jupiter | Transformationsystem in filamentösen fungiziden chrysosporium-wirtszellen |
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| WO2001020007A1 (en) * | 1999-09-13 | 2001-03-22 | Concordia University | A multifunctional system for the efficient manipulation of protein expression in filamentous fungi and method using same |
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| CN116135979A (zh) * | 2021-11-16 | 2023-05-19 | 中国科学院天津工业生物技术研究所 | 一种基于流式细胞术的免涂板操作的菌株工程改造的方法及应用 |
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Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4775622A (en) * | 1982-03-08 | 1988-10-04 | Genentech, Inc. | Expression, processing and secretion of heterologous protein by yeast |
| NO840200L (no) * | 1983-01-28 | 1984-07-30 | Cefus Corp | Glukoamylase cdna. |
| DK601984D0 (da) * | 1984-12-14 | 1984-12-14 | Hansens Lab | Fremgangsmaade til fremstilling af genprodukter |
| DE3650783T2 (de) * | 1985-04-15 | 2004-07-15 | Dsm Ip Assets B.V. | Verwendung des Glucoamylasepromotors aus Apergillus |
| DK122686D0 (da) * | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| FI872102A (fi) * | 1986-06-06 | 1987-12-07 | Panlabs Inc | Expressionssystem hos filamentala fungi. |
-
1986
- 1986-08-27 AU AU62008/86A patent/AU607398B2/en not_active Expired
- 1986-08-27 DE DE3650202T patent/DE3650202T3/de not_active Expired - Lifetime
- 1986-08-27 EP EP86306624A patent/EP0215594B2/en not_active Expired - Lifetime
- 1986-08-27 AT AT86306624T patent/ATE117020T1/de not_active IP Right Cessation
- 1986-08-27 NZ NZ217373A patent/NZ217373A/xx unknown
- 1986-08-27 EP EP94201751A patent/EP0625577A1/en not_active Withdrawn
- 1986-08-28 IE IE231086A patent/IE65794B1/en not_active IP Right Cessation
- 1986-08-28 IE IE950656A patent/IE950656L/xx unknown
- 1986-08-28 CA CA000516993A patent/CA1341532C/en not_active Expired - Fee Related
- 1986-08-29 DK DK414486A patent/DK175460B1/da not_active IP Right Cessation
-
1996
- 1996-02-27 JP JP8040000A patent/JPH08280388A/ja active Pending
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1998
- 1998-03-31 HK HK98102696A patent/HK1003579A1/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| ATE117020T1 (de) | 1995-01-15 |
| JPH08280388A (ja) | 1996-10-29 |
| IE862310L (en) | 1987-02-28 |
| HK1003579A1 (en) | 1998-10-30 |
| DK175460B1 (da) | 2004-11-01 |
| EP0215594B2 (en) | 2003-10-15 |
| NZ217373A (en) | 1990-10-26 |
| EP0215594B1 (en) | 1995-01-11 |
| DE3650202D1 (de) | 1995-02-23 |
| IE950656L (en) | 1987-02-28 |
| DE3650202T2 (de) | 1995-06-01 |
| EP0625577A1 (en) | 1994-11-23 |
| AU6200886A (en) | 1987-03-05 |
| EP0215594A3 (en) | 1988-08-31 |
| IE65794B1 (en) | 1995-11-15 |
| DK414486D0 (da) | 1986-08-29 |
| DE3650202T3 (de) | 2004-06-03 |
| AU607398B2 (en) | 1991-03-07 |
| DK414486A (da) | 1987-03-01 |
| EP0215594A2 (en) | 1987-03-25 |
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