CA1340733C - Regulatory region for heterologous gene expression in yeast - Google Patents

Regulatory region for heterologous gene expression in yeast

Info

Publication number
CA1340733C
CA1340733C CA000494002A CA494002A CA1340733C CA 1340733 C CA1340733 C CA 1340733C CA 000494002 A CA000494002 A CA 000494002A CA 494002 A CA494002 A CA 494002A CA 1340733 C CA1340733 C CA 1340733C
Authority
CA
Canada
Prior art keywords
dna
gly
ser
dna fragment
pro
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA000494002A
Other languages
English (en)
French (fr)
Inventor
David W. Stroman
Paul F. Brust
Steven B. Ellis
Thomas R. Gingeras
Michael M. Harpold
Juerg F. Tschopp
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Research Corp Technologies Inc
Original Assignee
Research Corp Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27099454&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA1340733(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US06/666,391 external-priority patent/US4808537A/en
Application filed by Research Corp Technologies Inc filed Critical Research Corp Technologies Inc
Application granted granted Critical
Publication of CA1340733C publication Critical patent/CA1340733C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
CA000494002A 1984-10-30 1985-10-28 Regulatory region for heterologous gene expression in yeast Expired - Lifetime CA1340733C (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US666,391 1984-10-30
US06/666,391 US4808537A (en) 1984-10-30 1984-10-30 Methanol inducible genes obtained from pichia and methods of use
US06/780,102 US4855231A (en) 1984-10-30 1985-09-25 Regulatory region for heterologous gene expression in yeast
US780,102 1985-09-25

Publications (1)

Publication Number Publication Date
CA1340733C true CA1340733C (en) 1999-09-14

Family

ID=27099454

Family Applications (1)

Application Number Title Priority Date Filing Date
CA000494002A Expired - Lifetime CA1340733C (en) 1984-10-30 1985-10-28 Regulatory region for heterologous gene expression in yeast

Country Status (23)

Country Link
US (1) US4855231A (enExample)
EP (2) EP0483115A3 (enExample)
JP (5) JP2502508B2 (enExample)
KR (1) KR930001117B1 (enExample)
CN (1) CN1011243B (enExample)
AR (1) AR242988A1 (enExample)
AT (1) ATE83263T1 (enExample)
AU (1) AU572002B2 (enExample)
CA (1) CA1340733C (enExample)
DD (1) DD254211A5 (enExample)
DE (1) DE3586889T2 (enExample)
DK (1) DK496385A (enExample)
ES (1) ES8609458A1 (enExample)
FI (1) FI94427C (enExample)
GR (1) GR852611B (enExample)
HU (1) HU205627B (enExample)
IE (1) IE930804L (enExample)
IL (1) IL76765A (enExample)
NO (1) NO178076C (enExample)
NZ (1) NZ213842A (enExample)
PL (1) PL158965B1 (enExample)
PT (1) PT81399B (enExample)
YU (1) YU46695B (enExample)

Families Citing this family (117)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5741672A (en) * 1984-07-27 1998-04-21 Unilever Patent Holdings B.V. Expression and production of polypeptides using the promoters of the hansenula polymorpha MOX and DAS genes
US4879231A (en) * 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US4882279A (en) * 1985-10-25 1989-11-21 Phillips Petroleum Company Site selective genomic modification of yeast of the genus pichia
US4818700A (en) * 1985-10-25 1989-04-04 Phillips Petroleum Company Pichia pastoris argininosuccinate lyase gene and uses thereof
US5032516A (en) * 1985-10-25 1991-07-16 Phillips Petroleum Company Pichia pastoris alcohol oxidase II regulatory region
US4895800A (en) * 1985-11-26 1990-01-23 Phillips Petroleum Company Yeast production of hepatitis B surface antigen
IL81817A0 (en) * 1986-03-14 1987-10-20 Phillips Petroleum Co Methanol and glucose responsive yeast regulatory regions
ZA872534B (en) * 1986-05-08 1987-11-25 Phillips Petroleum Co Yeast production of streptokinase
IL82935A0 (en) * 1986-08-12 1987-12-20 Phillips Petroleum Co Secretion of heterologous proteins from yeast
US5002876A (en) * 1986-09-22 1991-03-26 Phillips Petroleum Company Yeast production of human tumor necrosis factor
DE3789866T2 (de) * 1987-07-17 1994-09-22 Rhein Biotech Gesellschaft Fuer Neue Biotechnologische Prozesse Und Produkte Mbh, 40595 Duesseldorf DNA-Moleküle, die für FMDH-Kontrollabschnitte und Strukturgene für ein Protein mit FMDH-Aktivität kodieren, sowie deren Anwendung.
ATE248925T1 (de) * 1988-01-05 2003-09-15 Roche Diagnostics Gmbh Verfahren zur herstellung von proteinen oder proteinhaltigen genprodukten
FI891695A7 (fi) * 1988-04-11 1989-10-12 Phillips Petroleum Co Lohen kasvuhormoonin ilmentäminen sukuun Pichia kuuluvassa metylotroofisessa hiivassa
IL90021A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Process for the production of interferon
IL89991A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of hepatitis b pres2 protein in methylotrophic yeasts
IL89989A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of human interleukin-2 in methylotrophic yeasts
IL89992A0 (en) 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of human serum albumin in methylotrophic yeasts
IL89993A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of the hiv 24kda gag protein in methylotrophic yeasts
ATE111524T1 (de) * 1988-12-20 1994-09-15 Rhein Biotech Ges Fuer Biotech Mutanter stamm von einem methylotrophen organismus und verfahren zur herstellung eines proteins in methylotrophen organismus.
AU5196290A (en) * 1989-02-13 1990-09-05 Salk Institute Biotechnology/Industrial Associates, Inc., The Production of superoxide dismutase in pichia pastoris yeast cells
GB8924883D0 (en) * 1989-11-03 1989-12-20 Shell Int Research Expression vector and polypeptide synthesis
US5264554A (en) * 1990-01-19 1993-11-23 The Blood Center Of Southeastern Wisconsin, Inc. Platelet cell adhesion molecule and variants thereof
US6440681B1 (en) 1990-04-03 2002-08-27 Merck & Co., Inc. Methods for identifying agonists and antagonists for human neuronal nicotinic acetylcholine receptors
US5369028A (en) * 1990-04-03 1994-11-29 The Salk Institute Biotechnology/Industrial Associates, Inc. DNA and mRNA encoding human neuronal nicotinic acetylcholine receptor compositions and cells transformed with same
US5258302A (en) * 1990-07-03 1993-11-02 The Salk Institute Biotechnology/Industrial Associates, Inc. DNA for expression of aprotinin in methylotrophic yeast cells
CA2090969C (en) * 1990-09-04 2001-08-21 Russell Arthur Brierley Production of insulin-like growth factor-1 in methylotrophic yeast cells
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
US5268273A (en) * 1990-12-14 1993-12-07 Phillips Petroleum Company Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same
WO1992013951A1 (en) * 1991-02-04 1992-08-20 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of human serum albumin in methylotrophic yeast cells
AU661844B2 (en) * 1991-04-01 1995-08-10 Merck Sharp & Dohme Corp. Genes which influence (pichia) proteolytic activity, and uses therefor
CA2058820C (en) * 1991-04-25 2003-07-15 Kotikanyad Sreekrishna Expression cassettes and vectors for the secretion of human serum albumin in pichia pastoris cells
US5330901A (en) * 1991-04-26 1994-07-19 Research Corporation Technologies, Inc. Expression of human serum albumin in Pichia pastoris
US5834262A (en) * 1992-01-06 1998-11-10 E. I. Du Pont De Nemours And Company Oxidation of glycolic acid to glyoxylic acid using a microbial cell transformant as catalyst
ATE141330T1 (de) * 1992-02-28 1996-08-15 Suntory Ltd Neuer vektor, einen von methanol und/oder glyzerin induzierbaren promotor enthaltend
US5708141A (en) * 1992-05-11 1998-01-13 Corvas International, Inc. Neutrophil inhibitors
EP0690921B1 (en) * 1993-03-03 1997-05-28 E.I. Du Pont De Nemours And Company Production of glycolate oxidase in methylotrophic yeast
JP3628691B2 (ja) * 1993-03-08 2005-03-16 メルク エンド カンパニー インコーポレーテッド ヒト神経細胞性ニコチン性アセチルコリン受容体組成物およびそれらの使用方法
WO1994021802A1 (en) * 1993-03-25 1994-09-29 Biosource Genetics Corporation PICHIA PASTORIS ALCOHOL OXIDASE ZZA1 and $i(ZZA2)
JPH08509607A (ja) 1993-04-20 1996-10-15 ザ ソールク インスチチュート バイオテクノロジー／インダストリアル アソシエイツ，インコーポレイテッド ヒトｎ−メチル−ｄ−アスパラギン酸受容体サブユニット、これをコードする核酸およびその用途
US5912122A (en) 1993-06-04 1999-06-15 Sibia Neurosciences, Inc. Nucleic acids encoding and method for detecting nucleic acid encoding human metabotropic glutamate receptor subtype mGluR6
US6001581A (en) 1993-06-04 1999-12-14 Sibia Neurosciences, Inc. Cation-based bioassay using human metabotropic glutamate receptors
US5521297A (en) 1993-06-04 1996-05-28 Salk Institute Biotechnology/Industrial Associates Nucleic acids encoding human metabotropic glutamate receptors
US6495343B1 (en) 1993-06-18 2002-12-17 The Salk Institute For Biological Studies Cloning and recombinant production of CRF receptor(s)
US6638905B2 (en) 1993-06-18 2003-10-28 The Salk Institute For Biological Studies Cloning and recombinant production of CFR receptor(s)
GB2287941B (en) * 1993-11-08 1998-01-28 Salk Inst Biotech Ind Human alpha2 neuronal nicotinic acetylcholine receptor compositions and methods employing same
US5453161A (en) * 1993-11-12 1995-09-26 Enichem S.P.A. Polyamic acid to polyimide conversion by microwave heating
CA2202351A1 (en) * 1994-10-18 1996-04-25 George Phillip Vlasuk Nematode-extracted serine protease inhibitors and anticoagulant proteins
US5872098A (en) * 1995-06-05 1999-02-16 Corvas International, Inc. Nematode-extracted anticoagulant protein
US6090916A (en) * 1994-10-18 2000-07-18 Corvas International, Inc. Nematode-extracted serine protease inhibitors and anticoagulant proteins
US5866543A (en) * 1995-06-05 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5945275A (en) * 1994-10-18 1999-08-31 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5863894A (en) * 1995-06-05 1999-01-26 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5866542A (en) * 1994-10-18 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
US6143557A (en) * 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US6720140B1 (en) * 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US6485967B1 (en) 1995-06-07 2002-11-26 Merck & Co., Inc. Human neuronal nicotinic acetylcholine receptor α6 and β3 nucleic acid
WO1997017451A2 (en) * 1995-11-09 1997-05-15 Zymogenetics, Inc. Production of gad65 in methylotrophic yeast
MX9605082A (es) 1996-10-24 1998-04-30 Univ Autonoma De Nuevo Leon Levaduras metilotroficas modificadas geneticamente para la produccion y secrecion de hormona de crecimiento humano.
EP1659173A1 (en) 1997-10-01 2006-05-24 DSMIP Assets B.V. Protein production process
US7351578B2 (en) * 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
DE69836092T2 (de) * 1997-10-24 2007-05-10 Invitrogen Corp., Carlsbad Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen
CN101125873A (zh) * 1997-10-24 2008-02-20 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
EP1094081B1 (en) * 1998-07-06 2005-09-21 Tosoh Corporation Fused protein with direct bond of il-6-receptor to il-6
JP4524816B2 (ja) * 1998-07-06 2010-08-18 東ソー株式会社 Ｉｌ−６レセプター・ｉｌ−６直結融合蛋白質
WO2000012687A1 (en) * 1998-08-28 2000-03-09 Invitrogen Corporation System for the rapid manipulation of nucleic acid sequences
US6780615B1 (en) 1998-12-31 2004-08-24 Genway Biotech Inc. Production of recombinant monellin using methylotrophic yeast expression system
US6720174B1 (en) 1999-01-28 2004-04-13 Novozymes A/S Phytases
DE60042969D1 (de) * 1999-03-02 2009-10-29 Life Technologies Corp Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren
US7504253B2 (en) * 1999-06-11 2009-03-17 The Burnham Institute For Medical Research Nucleic acid encoding proteins involved in protein degradation, products and methods related thereof
US6440414B1 (en) * 1999-10-01 2002-08-27 Amgen Inc. Pharmaceutical compositions of fibrinolytic agent
US6261820B1 (en) * 1999-10-01 2001-07-17 Amgen Inc. Fibronolytically active polypeptide
AU1591701A (en) 1999-11-12 2001-06-06 Fibrogen, Inc. Recombinant gelatins
ATE391789T1 (de) 1999-12-10 2008-04-15 Invitrogen Corp Verwendung einer vielzahl von rekombinationsstellen mit einzigartiger spezifität beim rekombinatorischen klonen
US7033776B2 (en) * 1999-12-17 2006-04-25 Amgen Inc. Method for treatment of indwelling catheter occlusion using fibrinolytic metalloproteinases
WO2001077351A1 (en) * 2000-04-07 2001-10-18 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Vectors and methods for dual protein expression in pichia pastoris and escherichia coli
US7244560B2 (en) * 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7598055B2 (en) * 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
US7029872B2 (en) * 2000-06-28 2006-04-18 Glycofi, Inc Methods for producing modified glycoproteins
US7863020B2 (en) 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
US7625756B2 (en) 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
US8697394B2 (en) * 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
US7449308B2 (en) 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
EP2028275A3 (en) 2000-06-30 2009-05-06 VIB vzw Protein glycosylation modification in pichia pastoris
US7009045B2 (en) * 2000-07-14 2006-03-07 Archer-Daniels-Midland Company Transformation systems for flavinogenic yeast
US7198924B2 (en) * 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
WO2002081650A2 (en) * 2001-04-05 2002-10-17 The Board Of Regents Of The University Of Nebraska Alcohol oxidase 1 regulatory nucleotide sequences for heterologous gene expression in yeast
CA2448505A1 (en) * 2001-05-21 2002-11-28 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
US6645739B2 (en) 2001-07-26 2003-11-11 Phoenix Pharmacologies, Inc. Yeast expression systems, methods of producing polypeptides in yeast, and compositions relating to same
CA2482485A1 (en) * 2002-04-15 2003-10-30 Merck & Co., Inc. Matrix analysis of gene expression in cells (magec)
US7252933B2 (en) 2002-06-26 2007-08-07 Flanders Interuniversity Institute For Biotechnology Protein glycosylation modification in methylotrophic yeast
US20040204953A1 (en) * 2002-07-17 2004-10-14 Lincoln Muir Subscription based systems, methods and components for providing genomic and proteomic products and services
US7118901B2 (en) * 2002-12-18 2006-10-10 Roche Diagnostics Operations, Inc. Recombinant bovine pancreatic desoxyribonuclease I with high specific activity
ATE387494T1 (de) * 2002-12-20 2008-03-15 Hoffmann La Roche Hitzelabile desoxyribonuklease i-varianten
US7332299B2 (en) 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
EP1460425A1 (en) * 2003-03-17 2004-09-22 Boehringer Mannheim Gmbh Deglycosylated enzymes for conjugates
DK1673457T3 (da) 2003-08-25 2011-09-05 Funzyme Biotechnologies Sa Nye svampeproteiner og nukleinsyrer, der koder for disse
US8293503B2 (en) 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
DE602004027538D1 (de) 2003-12-01 2010-07-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
ES2337684T3 (es) * 2003-12-05 2010-04-28 F. Hoffmann-La Roche Ag Carboxipeptidasa b recombinante y su purificacion.
ATE517919T1 (de) * 2005-09-14 2011-08-15 Sanofi Aventis Deutschland Spaltung von insulinvorstufen durch eine trypsinvariante
AT504588B1 (de) * 2006-11-16 2008-08-15 Univ Graz Tech Nukleinsäure-promotor
BRPI0910286A2 (pt) * 2008-03-03 2015-09-29 Abbott Lab métodos para transformação de levedura
WO2010068904A2 (en) 2008-12-12 2010-06-17 E. I. Du Pont De Nemours And Company Process for making linear dicarboxylic acids from renewable resources
EP2258855A1 (en) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expression sequences
EP2515932A2 (en) 2009-12-21 2012-10-31 Centre Hospitalier Universitaire Vaudois (CHUV) Synergic action of a prolyl protease and tripeptidyl proteases
CN110408644A (zh) 2011-05-20 2019-11-05 奥尔德生物控股有限责任公司 多亚基蛋白质例如抗体在转化的微生物例如巴斯德毕赤酵母中的高纯度产生
SG2014011878A (en) 2011-08-19 2014-04-28 Alderbio Holdings Llc Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as a antibodies in transformed microbes such as pichia pastoris
KR102128957B1 (ko) 2011-10-07 2020-07-02 론자 리미티드 조절가능한 프로모터
SG11201507557UA (en) 2013-03-15 2015-10-29 Lonza Ag Constitutive promoter
US9150870B2 (en) 2013-03-15 2015-10-06 Lonza Ltd. Constitutive promoter
TWI636136B (zh) 2013-03-15 2018-09-21 艾爾德生物製藥股份有限公司 在酵母菌及其它轉型細胞中多肽高產量表現用之溫度變動技術
WO2014145744A1 (en) 2013-03-15 2014-09-18 Alder Biopharmaceuticals, Inc. Antibody purification and purity monitoring
TWI657094B (zh) 2013-03-21 2019-04-21 全民科學與工業研究機構 三螺旋蛋白質之純化
WO2014179260A2 (en) 2013-04-30 2014-11-06 Donald Danforth Plant Science Center Antifungal plant proteins, peptides, and methods of use
TWI738631B (zh) 2013-09-09 2021-09-11 德商贏創運營有限公司 經修飾之細菌類膠原蛋白蛋白質
WO2016120764A1 (en) 2015-01-26 2016-08-04 Centre Hospitalier Universitaire Vaudois (Chuv) Aspergillus oryzae prolyl endopeptidases and use thereof in degradation of polypeptides
WO2021126129A1 (en) * 2019-12-19 2021-06-24 Atatürk Üni̇versi̇tesi̇ Bi̇li̇msel Araştirma Projeleri̇ Bi̇ri̇mi̇ A method for recombinant protein production

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK368882A (da) * 1981-08-25 1983-02-26 Alan John Kingsman Expessions vektorer
IE54592B1 (en) * 1982-03-08 1989-12-06 Genentech Inc Anumal interferons, processes involved in their production, compositions containing them, dna sequences coding therefor and espression vehicles containing such sequences and cells transformed thereby
EP0096910B1 (en) * 1982-05-19 1989-04-19 Unilever N.V. Yeast of the genus kluyveromyces modified for the expression of preprothaumatin or its various allelic and modified forms or their maturation forms
WO1984001153A1 (en) * 1982-09-15 1984-03-29 Collaborative Res Inc Production of interferon in yeast by use of invertase promoter
EP0423890A3 (en) * 1984-07-27 1991-07-03 Unilever Nv Use of oxidoreductases in bleaching and/or detergent compositions and their preparation by microorganisms engineered by recombinant dna technology
US4885242A (en) * 1984-10-30 1989-12-05 Phillips Petroleum Company Genes from pichia histidine pathway and uses thereof
US4837148A (en) * 1984-10-30 1989-06-06 Phillips Petroleum Company Autonomous replication sequences for yeast strains of the genus pichia
US4879231A (en) * 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia

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JP2552808B2 (ja) 1996-11-13
YU46695B (sh) 1994-04-05
IL76765A (en) 1991-06-30
PT81399B (pt) 1987-11-11
ES548307A0 (es) 1986-09-01
IE930804L (en) 1986-04-30
AU4875285A (en) 1986-06-12
DK496385A (da) 1986-05-01
EP0483115A2 (en) 1992-04-29
JP2502508B2 (ja) 1996-05-29
JPS61173781A (ja) 1986-08-05
PL256012A1 (en) 1986-09-23
ATE83263T1 (de) 1992-12-15
HUT38678A (en) 1986-06-30
DK496385D0 (da) 1985-10-29
EP0483115A3 (en) 1992-05-06
NO178076C (no) 1996-01-17
FI854142L (fi) 1986-05-01
KR870003197A (ko) 1987-04-15
NO178076B (no) 1995-10-09
AU572002B2 (en) 1988-04-28
NZ213842A (en) 1991-11-26
AR242988A1 (es) 1993-06-30
PL158965B1 (pl) 1992-10-30
ES8609458A1 (es) 1986-09-01
JPH06233686A (ja) 1994-08-23
YU169585A (en) 1991-04-30
DE3586889T2 (de) 1993-04-15
CN85109718A (zh) 1986-08-20
JPH08196275A (ja) 1996-08-06
KR930001117B1 (ko) 1993-02-18
FI94427B (fi) 1995-05-31
JPH0698789A (ja) 1994-04-12
US4855231A (en) 1989-08-08
HU205627B (en) 1992-05-28
EP0183071A3 (en) 1987-09-23
JPH08173161A (ja) 1996-07-09
EP0183071B1 (en) 1992-12-09
NO854312L (no) 1986-05-02
JP2552807B2 (ja) 1996-11-13
PT81399A (en) 1985-11-01
EP0183071A2 (en) 1986-06-04
FI854142A0 (fi) 1985-10-23
DD254211A5 (de) 1988-02-17
GR852611B (enExample) 1986-03-04
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