CA1082577A - Homogenous specific binding assay method and composition and device for use therein - Google Patents
Homogenous specific binding assay method and composition and device for use thereinInfo
- Publication number
- CA1082577A CA1082577A CA249,745A CA249745A CA1082577A CA 1082577 A CA1082577 A CA 1082577A CA 249745 A CA249745 A CA 249745A CA 1082577 A CA1082577 A CA 1082577A
- Authority
- CA
- Canada
- Prior art keywords
- reaction
- ligand
- specific binding
- reactant
- labeling substance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
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- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- XMHIUKTWLZUKEX-UHFFFAOYSA-N hexacosanoic acid Chemical compound CCCCCCCCCCCCCCCCCCCCCCCCCC(O)=O XMHIUKTWLZUKEX-UHFFFAOYSA-N 0.000 description 1
- FUZZWVXGSFPDMH-UHFFFAOYSA-N hexanoic acid Chemical compound CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 description 1
- VWQWXZAWFPZJDA-CGVGKPPMSA-N hydrocortisone succinate Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)COC(=O)CCC(O)=O)[C@@H]4[C@@H]3CCC2=C1 VWQWXZAWFPZJDA-CGVGKPPMSA-N 0.000 description 1
- 229960004337 hydroquinone Drugs 0.000 description 1
- 239000005457 ice water Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000002329 infrared spectrum Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 229940029329 intrinsic factor Drugs 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- ODBLHEXUDAPZAU-UHFFFAOYSA-N isocitric acid Chemical compound OC(=O)C(O)C(C(O)=O)CC(O)=O ODBLHEXUDAPZAU-UHFFFAOYSA-N 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 229940049920 malate Drugs 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- GBMDVOWEEQVZKZ-UHFFFAOYSA-N methanol;hydrate Chemical compound O.OC GBMDVOWEEQVZKZ-UHFFFAOYSA-N 0.000 description 1
- NQMRYBIKMRVZLB-UHFFFAOYSA-N methylamine hydrochloride Chemical compound [Cl-].[NH3+]C NQMRYBIKMRVZLB-UHFFFAOYSA-N 0.000 description 1
- WZVXLJYENHHFPD-UHFFFAOYSA-N methylaminomethanol;hydrochloride Chemical compound Cl.CNCO WZVXLJYENHHFPD-UHFFFAOYSA-N 0.000 description 1
- 125000001570 methylene group Chemical group [H]C([H])([*:1])[*:2] 0.000 description 1
- 125000000325 methylidene group Chemical class [H]C([H])=* 0.000 description 1
- 230000003228 microsomal effect Effects 0.000 description 1
- 150000004682 monohydrates Chemical class 0.000 description 1
- 229960005181 morphine Drugs 0.000 description 1
- XNLFFGIJJKYYIM-UHFFFAOYSA-N morpholin-4-yl propane-1-sulfonate Chemical compound CCCS(=O)(=O)ON1CCOCC1 XNLFFGIJJKYYIM-UHFFFAOYSA-N 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 229940101270 nicotinamide adenine dinucleotide (nad) Drugs 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- KHPXUQMNIQBQEV-UHFFFAOYSA-N oxaloacetic acid Chemical compound OC(=O)CC(=O)C(O)=O KHPXUQMNIQBQEV-UHFFFAOYSA-N 0.000 description 1
- 230000033116 oxidation-reduction process Effects 0.000 description 1
- YPZRWBKMTBYPTK-UHFFFAOYSA-N oxidized gamma-L-glutamyl-L-cysteinylglycine Natural products OC(=O)C(N)CCC(=O)NC(C(=O)NCC(O)=O)CSSCC(C(=O)NCC(O)=O)NC(=O)CCC(N)C(O)=O YPZRWBKMTBYPTK-UHFFFAOYSA-N 0.000 description 1
- 239000001301 oxygen Chemical group 0.000 description 1
- 229940094443 oxytocics prostaglandins Drugs 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 229930029653 phosphoenolpyruvate Natural products 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Chemical group 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- FYRHIOVKTDQVFC-UHFFFAOYSA-M potassium phthalimide Chemical compound [K+].C1=CC=C2C(=O)[N-]C(=O)C2=C1 FYRHIOVKTDQVFC-UHFFFAOYSA-M 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 150000003180 prostaglandins Chemical class 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 150000003222 pyridines Chemical class 0.000 description 1
- 238000012113 quantitative test Methods 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 238000001953 recrystallisation Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 229940043267 rhodamine b Drugs 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- DUIOPKIIICUYRZ-UHFFFAOYSA-N semicarbazide Chemical compound NNC(N)=O DUIOPKIIICUYRZ-UHFFFAOYSA-N 0.000 description 1
- 150000007659 semicarbazones Chemical class 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- BHZOKUMUHVTPBX-UHFFFAOYSA-M sodium acetic acid acetate Chemical compound [Na+].CC(O)=O.CC([O-])=O BHZOKUMUHVTPBX-UHFFFAOYSA-M 0.000 description 1
- JVBXVOWTABLYPX-UHFFFAOYSA-L sodium dithionite Chemical compound [Na+].[Na+].[O-]S(=O)S([O-])=O JVBXVOWTABLYPX-UHFFFAOYSA-L 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000011973 solid acid Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 1
- 229940014800 succinic anhydride Drugs 0.000 description 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Chemical group 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 150000003568 thioethers Chemical class 0.000 description 1
- 229940034208 thyroxine Drugs 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- DQWPFSLDHJDLRL-UHFFFAOYSA-N triethyl phosphate Chemical compound CCOP(=O)(OCC)OCC DQWPFSLDHJDLRL-UHFFFAOYSA-N 0.000 description 1
- ZMANZCXQSJIPKH-UHFFFAOYSA-O triethylammonium ion Chemical compound CC[NH+](CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-O 0.000 description 1
- 229940035722 triiodothyronine Drugs 0.000 description 1
- 125000002264 triphosphate group Chemical group [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 235000019163 vitamin B12 Nutrition 0.000 description 1
- 239000011715 vitamin B12 Substances 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
- C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
- C07D209/44—Iso-indoles; Hydrogenated iso-indoles
- C07D209/48—Iso-indoles; Hydrogenated iso-indoles with oxygen atoms in positions 1 and 3, e.g. phthalimide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D237/00—Heterocyclic compounds containing 1,2-diazine or hydrogenated 1,2-diazine rings
- C07D237/26—Heterocyclic compounds containing 1,2-diazine or hydrogenated 1,2-diazine rings condensed with carbocyclic rings or ring systems
- C07D237/30—Phthalazines
- C07D237/32—Phthalazines with oxygen atoms directly attached to carbon atoms of the nitrogen-containing ring
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
- C07D493/02—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains two hetero rings
- C07D493/10—Spiro-condensed systems
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D495/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms
- C07D495/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms in which the condensed system contains two hetero rings
- C07D495/04—Ortho-condensed systems
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US57200875A | 1975-04-28 | 1975-04-28 | |
| US572,008 | 1975-04-28 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1082577A true CA1082577A (en) | 1980-07-29 |
Family
ID=24285954
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA249,745A Expired CA1082577A (en) | 1975-04-28 | 1976-04-07 | Homogenous specific binding assay method and composition and device for use therein |
| CA249,744A Expired CA1078712A (en) | 1975-04-28 | 1976-04-07 | Heterogenous specific binding assay method and means for use therein |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA249,744A Expired CA1078712A (en) | 1975-04-28 | 1976-04-07 | Heterogenous specific binding assay method and means for use therein |
Country Status (23)
| Country | Link |
|---|---|
| JP (6) | JPS6052378B2 (en, 2012) |
| AT (1) | AT357685B (en, 2012) |
| AU (2) | AU509182B2 (en, 2012) |
| BE (1) | BE841179A (en, 2012) |
| BR (1) | BR7602561A (en, 2012) |
| CA (2) | CA1082577A (en, 2012) |
| CH (1) | CH635438A5 (en, 2012) |
| DD (1) | DD125231A5 (en, 2012) |
| DE (3) | DE2618511C3 (en, 2012) |
| DK (3) | DK149969C (en, 2012) |
| ES (1) | ES447378A1 (en, 2012) |
| FI (1) | FI68324C (en, 2012) |
| FR (1) | FR2332533A1 (en, 2012) |
| GB (2) | GB1552607A (en, 2012) |
| HU (1) | HU179542B (en, 2012) |
| IE (1) | IE42544B1 (en, 2012) |
| IL (1) | IL49354A (en, 2012) |
| IN (1) | IN142734B (en, 2012) |
| IT (1) | IT1064132B (en, 2012) |
| LU (1) | LU74834A1 (en, 2012) |
| NL (1) | NL181281C (en, 2012) |
| SE (3) | SE440280B (en, 2012) |
| ZA (1) | ZA762030B (en, 2012) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7732153B2 (en) | 2006-05-09 | 2010-06-08 | Beckman Coulter, Inc. | Nonseparation assay methods |
| US7799534B2 (en) | 2006-05-09 | 2010-09-21 | Beckman Coulter, Inc. | Nonseparation assay methods |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL51668A (en) | 1977-03-16 | 1981-12-31 | Israel State | Analytical method for the quantitative determination of immunogens and antibodies and a kit therefor |
| SE7811630L (sv) * | 1977-11-17 | 1979-05-18 | Welsh Nat School Med | Metod for uppteckt eller mengdbestemning av substanser med anvendning av merkningsteknik |
| USRE34394E (en) * | 1978-01-23 | 1993-09-28 | Baxter Diagnostics Inc. | Method and composition for double receptor, specific binding assays |
| AU531777B2 (en) * | 1978-04-05 | 1983-09-08 | Syva Co. | Label/solid conjugate immunoassay system |
| US4233402A (en) * | 1978-04-05 | 1980-11-11 | Syva Company | Reagents and method employing channeling |
| FR2422956A1 (fr) * | 1978-04-13 | 1979-11-09 | Pasteur Institut | Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede |
| US5605800A (en) * | 1978-04-13 | 1997-02-25 | Institut Pasteur | Method of detecting and characterizing a nucleic acid or a sequence of the latter, and enzymatic reactant for the application of this method |
| DE2924249C2 (de) * | 1978-06-22 | 1989-04-27 | Miles, Inc., Elkhart, Ind., Us | Spezifische bindungs-untersuchungsmethode zur bestimmung eines liganden in einem fluessigen medium und reagentien zur durchfuehrung dieser methode |
| IL57571A (en) * | 1978-06-22 | 1983-03-31 | Miles Lab | Flavin adenine dinucleotide-labeled conjugates and their use in a homogeneous immunoassay method for determining haptens |
| US4334069A (en) * | 1978-07-24 | 1982-06-08 | Miles Laboratories, Inc. | Chemiluminescent phthalhydrazide-labeled hapten conjugates |
| US4225485A (en) * | 1978-07-24 | 1980-09-30 | Miles Laboratories, Inc. | Chemiluminescent naphthalene-1,2-dicarboxylic acid hydrazide-labeled polypeptides and proteins |
| US4228237A (en) * | 1978-09-21 | 1980-10-14 | Calbiochem-Behring Corp. | Methods for the detection and determination of ligands |
| US4288538A (en) | 1979-01-31 | 1981-09-08 | Baxter Travenol Laboratories, Inc. | Test method and composition therefor |
| US4273866A (en) * | 1979-02-05 | 1981-06-16 | Abbott Laboratories | Ligand analog-irreversible enzyme inhibitor conjugates and methods for use |
| GB2059421A (en) * | 1979-10-03 | 1981-04-23 | Self C H | Assay method and reagents therefor |
| US4446231A (en) * | 1979-10-03 | 1984-05-01 | Self Colin H | Immunoassay using an amplified cyclic detection system |
| US4259232A (en) * | 1979-10-23 | 1981-03-31 | Miles Laboratories, Inc. | Flavin adenine dinucleotide-labeled protein and polypeptide conjugates |
| AT367796B (de) * | 1979-10-29 | 1982-07-26 | List Hans | Verfahren zur bestimmung einer stoffkonzentration in einer loesung |
| WO1981001414A1 (en) * | 1979-11-19 | 1981-05-28 | Charles Hospital Dev | An improved method of non homogenous enzyme immunoassay |
| US4299916A (en) * | 1979-12-26 | 1981-11-10 | Syva Company | Preferential signal production on a surface in immunoassays |
| DK256581A (da) * | 1980-06-13 | 1981-12-14 | Nat Res Dev | Bindingsanalyse |
| EP0049606A1 (en) * | 1980-10-02 | 1982-04-14 | Colin Henry Self | Detection method, use and diagnostic aid |
| CA1183450A (en) * | 1980-10-30 | 1985-03-05 | Alfred C. Greenquist | Homogeneous specific binding assay device and method |
| US4404278A (en) * | 1980-11-24 | 1983-09-13 | Syva Company | Methods and compositions for assaying for the production of 1,4-dihydropyridyl |
| US4378458A (en) * | 1981-03-30 | 1983-03-29 | Baker Instruments Corporation | Novel chromogenic and/or fluorogenic substrates for monitoring catalytic or enzymatic activity |
| US4366243A (en) | 1981-04-17 | 1982-12-28 | Miles Laboratories, Inc. | Stabilization of glucose oxidase apoenzyme |
| CA1219824A (en) * | 1981-04-17 | 1987-03-31 | David C. Ward | Modified nucleotides and methods of preparing and using same |
| ATE48140T1 (de) * | 1981-04-17 | 1989-12-15 | Univ Yale | Modifizierte nukleotide und verfahren zu ihrer herstellung und anwendung. |
| US4711955A (en) * | 1981-04-17 | 1987-12-08 | Yale University | Modified nucleotides and methods of preparing and using same |
| JPS5818167A (ja) * | 1981-07-24 | 1983-02-02 | Fuji Photo Film Co Ltd | 分析フイルム及びこれを用いる分析方法 |
| JPS5877843U (ja) * | 1981-11-20 | 1983-05-26 | 株式会社三協精機製作所 | テ−プレコ−ダ |
| GB2112779B (en) * | 1981-12-11 | 1986-10-15 | Welsh Nat School Med | Aryl acridinium esters as luminescent labelling materials |
| FR2519004B1 (fr) * | 1981-12-29 | 1985-09-27 | Pasteur Institut | Acide adenosine 5'-triphosphorique modifie et procede de dosage de substances biologiques susceptibles de fixer des produits de degradation de l'acide adenosine 5'-triphosphorique |
| FR2519005B1 (fr) * | 1981-12-29 | 1985-10-25 | Pasteur Institut | Fragments d'adn marques a l'une au moins de leurs extremites par des ribonucleotides modifies reconnaissables par des molecules affines et procede pour realiser une analyse de tels fragments d'adn |
| US4709016A (en) * | 1982-02-01 | 1987-11-24 | Northeastern University | Molecular analytical release tags and their use in chemical analysis |
| US5650270A (en) * | 1982-02-01 | 1997-07-22 | Northeastern University | Molecular analytical release tags and their use in chemical analysis |
| IL67289A (en) * | 1982-03-18 | 1985-12-31 | Miles Lab | Homogeneous immunoassay with labeled monoclonal anti-analyte |
| CA1223831A (en) * | 1982-06-23 | 1987-07-07 | Dean Engelhardt | Modified nucleotides, methods of preparing and utilizing and compositions containing the same |
| US4495281A (en) * | 1982-10-21 | 1985-01-22 | Miles Laboratories, Inc. | Tricyclic antidepressant drug immunogens, antibodies, labeled conjugates, and related derivatives |
| US4994373A (en) | 1983-01-27 | 1991-02-19 | Enzo Biochem, Inc. | Method and structures employing chemically-labelled polynucleotide probes |
| US4828979A (en) * | 1984-11-08 | 1989-05-09 | Life Technologies, Inc. | Nucleotide analogs for nucleic acid labeling and detection |
| EP0201211A1 (en) * | 1985-04-10 | 1986-11-12 | Whittaker Corporation | Method and compositions for visual solid phase immunoassays based on luminescent microspheric particles |
| JPS61271457A (ja) * | 1985-05-28 | 1986-12-01 | Olympus Optical Co Ltd | 免疫学的分析方法 |
| JPH06100599B2 (ja) * | 1985-06-27 | 1994-12-12 | 東亜医用電子株式会社 | 体液成分の測定方法 |
| JPS6321559A (ja) * | 1986-07-16 | 1988-01-29 | Matsushita Electric Ind Co Ltd | ハプテンの酵素免疫測定法 |
| DE3844954C2 (de) * | 1988-02-20 | 1998-07-16 | Hoechst Ag | Spezielle chemilumineszierende Acridinderivate und ihre Verwendung in Lumineszenzimmunoassays |
| US6002007A (en) * | 1988-02-20 | 1999-12-14 | Dade Behring Marburg Gmbh | Special chemiluminescent acridine derivatives and the use thereof in luminescence immunoassays |
| JP2525678B2 (ja) * | 1989-10-03 | 1996-08-21 | 富士写真フイルム株式会社 | 免疫測定装置 |
| US6596490B2 (en) | 2000-07-14 | 2003-07-22 | Applied Gene Technologies, Inc. | Nucleic acid hairpin probes and uses thereof |
| US6380377B1 (en) | 2000-07-14 | 2002-04-30 | Applied Gene Technologies, Inc. | Nucleic acid hairpin probes and uses thereof |
| CN100494399C (zh) | 2003-06-30 | 2009-06-03 | 清华大学 | 一种基于dna芯片的基因分型方法及其应用 |
| JP6214449B2 (ja) * | 2014-03-31 | 2017-10-18 | シスメックス株式会社 | キナーゼ活性の測定方法 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL154600B (nl) * | 1971-02-10 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen. |
| US3817837A (en) * | 1971-05-14 | 1974-06-18 | Syva Corp | Enzyme amplification assay |
| US3880934A (en) * | 1972-02-10 | 1975-04-29 | Syntex Inc | Nitrophenyloxy-butanediols |
| JPS5147347A (ja) * | 1974-10-21 | 1976-04-22 | Nippon Electric Co | Johoshorisochi |
| JPS5147348A (ja) * | 1974-10-21 | 1976-04-22 | Nippon Electric Co | Johoshorisochi |
-
1975
- 1975-09-30 IN IN1874/CAL/75A patent/IN142734B/en unknown
-
1976
- 1976-04-02 IE IE696/76A patent/IE42544B1/en unknown
- 1976-04-05 ZA ZA762030A patent/ZA762030B/xx unknown
- 1976-04-05 IL IL49354A patent/IL49354A/xx unknown
- 1976-04-07 CA CA249,745A patent/CA1082577A/en not_active Expired
- 1976-04-07 CA CA249,744A patent/CA1078712A/en not_active Expired
- 1976-04-21 CH CH497976A patent/CH635438A5/de not_active IP Right Cessation
- 1976-04-23 GB GB16610/76A patent/GB1552607A/en not_active Expired
- 1976-04-23 GB GB16609/76A patent/GB1548741A/en not_active Expired
- 1976-04-26 NL NLAANVRAGE7604420,A patent/NL181281C/xx not_active IP Right Cessation
- 1976-04-26 IT IT49195/76A patent/IT1064132B/it active
- 1976-04-26 LU LU74834A patent/LU74834A1/xx unknown
- 1976-04-26 FI FI761148A patent/FI68324C/fi not_active IP Right Cessation
- 1976-04-27 JP JP51047348A patent/JPS6052378B2/ja not_active Expired
- 1976-04-27 FR FR7612441A patent/FR2332533A1/fr active Granted
- 1976-04-27 DE DE2618511A patent/DE2618511C3/de not_active Expired
- 1976-04-27 AT AT309376A patent/AT357685B/de not_active IP Right Cessation
- 1976-04-27 AU AU13352/76A patent/AU509182B2/en not_active Expired
- 1976-04-27 JP JP51047347A patent/JPS604939B2/ja not_active Expired
- 1976-04-27 BE BE166499A patent/BE841179A/xx not_active IP Right Cessation
- 1976-04-27 AU AU13353/76A patent/AU502726B2/en not_active Expired
- 1976-04-27 BR BR2561/76A patent/BR7602561A/pt unknown
- 1976-04-27 HU HU76MI600A patent/HU179542B/hu unknown
- 1976-04-27 DK DK189176A patent/DK149969C/da not_active IP Right Cessation
- 1976-04-27 DE DE2660548A patent/DE2660548C2/de not_active Expired
- 1976-04-27 ES ES447378A patent/ES447378A1/es not_active Expired
- 1976-04-27 DD DD192528A patent/DD125231A5/xx unknown
- 1976-04-27 DE DE2618419A patent/DE2618419C2/de not_active Expired
- 1976-04-27 SE SE7604841A patent/SE440280B/xx not_active IP Right Cessation
-
1980
- 1980-10-21 SE SE8007396A patent/SE447510B/sv not_active IP Right Cessation
- 1980-10-21 SE SE8007397A patent/SE451895B/sv not_active IP Right Cessation
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1981
- 1981-12-23 DK DK576281A patent/DK150808C/da not_active IP Right Cessation
- 1981-12-23 DK DK576181A patent/DK158366C/da not_active IP Right Cessation
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1983
- 1983-07-07 JP JP58122463A patent/JPS5942453A/ja active Granted
- 1983-07-07 JP JP58122465A patent/JPS5951353A/ja active Granted
- 1983-07-07 JP JP58122466A patent/JPS5951354A/ja active Granted
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7732153B2 (en) | 2006-05-09 | 2010-06-08 | Beckman Coulter, Inc. | Nonseparation assay methods |
| US7799534B2 (en) | 2006-05-09 | 2010-09-21 | Beckman Coulter, Inc. | Nonseparation assay methods |
| US8377647B2 (en) | 2006-05-09 | 2013-02-19 | Beckman Coulter, Inc. | Nonseparation assay methods |
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