BRPI0714257A8 - "métodos para a cultura de células tronco embriónarias (es) de aves, para modificar geneticamente as células tronco embrionárias, para a obtenção de um pintinho quimérico, para a obtenção de um progênie do pintinho, para a analise do polimorfismo genético, meio de cultura para células tronco embrionárias de aves e meio de cultura". - Google Patents

"métodos para a cultura de células tronco embriónarias (es) de aves, para modificar geneticamente as células tronco embrionárias, para a obtenção de um pintinho quimérico, para a obtenção de um progênie do pintinho, para a analise do polimorfismo genético, meio de cultura para células tronco embrionárias de aves e meio de cultura".

Info

Publication number
BRPI0714257A8
BRPI0714257A8 BRPI0714257A BRPI0714257A BRPI0714257A8 BR PI0714257 A8 BRPI0714257 A8 BR PI0714257A8 BR PI0714257 A BRPI0714257 A BR PI0714257A BR PI0714257 A BRPI0714257 A BR PI0714257A BR PI0714257 A8 BRPI0714257 A8 BR PI0714257A8
Authority
BR
Brazil
Prior art keywords
embryonic stem
stem cells
cells
obtaining
culture
Prior art date
Application number
BRPI0714257A
Other languages
English (en)
Inventor
Valarche Isabelle
Batard Luc
Mehtali Majid
Original Assignee
Valneva
Vivalis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Valneva, Vivalis filed Critical Valneva
Publication of BRPI0714257A2 publication Critical patent/BRPI0714257A2/pt
Publication of BRPI0714257A8 publication Critical patent/BRPI0714257A8/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/30—Bird
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/13—Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2306—Interleukin-6 (IL-6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

MÉTODOS PARA A CULTURA DE CÉLULAS TRONCO EMBRIONÁRIAS (ES) DE AVES, PARA MODIFICAR GENETICAMENTE AS CÉLULAS TRONCO EMBRIONÁRIAS, PARA A OBTENÇÃO DE UM PINTINHO QUIMÉRICO, PARA A OBTENÇÃO DE UMA PROGÊNIE DO PINTINHO QUIMÉRICO, PARA A ANÁLISE DO POLIMORFISMO GENÉTICO, MEIO DE CULTURA PARA CÉLULAS TRONCO EMBRIONÁRIAS DE AVES E MEIO DE CULTURA. A presente invenção se refere a um método para a cultura de células embrionárias (ES) de aves, que compreende as etapas de: (a) suspender as células ES originadas do disco da blastoderme de ovo(s) de aves fertilizados, não incubados, em um meio de cultura basal suplementado com: o fator de crescimento semelhante à insulina 1 (IGF-1) e o fator neurotrófico ciliar (CNTF); e soro animal; e opcionalmente, pelo menos um fator do crescimento selecionado a partir do grupo que consiste em interleucina 6 (IL-6), receptor de interleucina-6 (IL-6R), fator de célula tronco (SCF), fator de crescimento de fibroblasto (FGF), fator inibitório de leucemia (LIF), interleucina 11 (IL-11 ), oncostatina e cardiotrofina; (b) inocular a suspensão de células ES obtidas na etapa (a) em uma camada de células alimentadoras (feeder cells) e ainda realizar a cultura de células ES por pelo menos entre 2 a 10 passagens; (c) opcionalmente, remover pelo menos um fator de crescimento selecionados a partir de SCF, FGF, IL-6, IL-6R, LIF, oncostatina e cadiotrofina e IL-11 do meio de cultura; (d) realizar ainda a cultura das células ES no meio da etapa (c) em uma camada de células alimentadoras.
BRPI0714257A 2006-08-09 2007-08-09 "métodos para a cultura de células tronco embriónarias (es) de aves, para modificar geneticamente as células tronco embrionárias, para a obtenção de um pintinho quimérico, para a obtenção de um progênie do pintinho, para a analise do polimorfismo genético, meio de cultura para células tronco embrionárias de aves e meio de cultura". BRPI0714257A8 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US83637806P 2006-08-09 2006-08-09
PCT/EP2007/058263 WO2008017704A1 (en) 2006-08-09 2007-08-09 Method of production of transgenic avian using embryonic stem cells

Publications (2)

Publication Number Publication Date
BRPI0714257A2 BRPI0714257A2 (pt) 2013-12-03
BRPI0714257A8 true BRPI0714257A8 (pt) 2017-05-09

Family

ID=38752394

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0714257A BRPI0714257A8 (pt) 2006-08-09 2007-08-09 "métodos para a cultura de células tronco embriónarias (es) de aves, para modificar geneticamente as células tronco embrionárias, para a obtenção de um pintinho quimérico, para a obtenção de um progênie do pintinho, para a analise do polimorfismo genético, meio de cultura para células tronco embrionárias de aves e meio de cultura".

Country Status (12)

Country Link
US (2) US8962311B2 (pt)
EP (2) EP2054507A1 (pt)
JP (1) JP5405302B2 (pt)
KR (1) KR101567095B1 (pt)
CN (1) CN101506354B (pt)
AU (1) AU2007283564B2 (pt)
BR (1) BRPI0714257A8 (pt)
CA (1) CA2660035A1 (pt)
MX (1) MX2009001477A (pt)
NZ (1) NZ574678A (pt)
RU (1) RU2473688C2 (pt)
WO (1) WO2008017704A1 (pt)

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WO2011156723A1 (en) 2010-06-11 2011-12-15 Regeneron Pharmaceuticals, Inc. Production of fertile xy female animals from xy es cells
EP2883951B1 (en) * 2012-06-15 2018-01-17 Kyokuto Pharmaceutical Industrial Co. Ltd Stem cell preservation medium, stem cell preservation method, and stem cell preservation system
US20140255447A1 (en) * 2013-03-05 2014-09-11 Biomune Company Production of avian embryo cells
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CN108004197B (zh) * 2017-12-14 2021-06-08 浙江海洋大学 一种乌贼胚胎细胞的原代培养方法及专有试剂
CN108782458B (zh) * 2018-07-02 2022-02-11 江苏威特凯鸽业有限公司 一种改良泰深鸽的培育方法
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CN114350599B (zh) * 2022-01-14 2024-01-19 内蒙古大学 一种增强牛胚胎干细胞的多能性和代谢活力的培养体系和培养方法
CN115088679A (zh) * 2022-07-20 2022-09-23 扬州大学 一种利用性腺细胞构建嵌合体鸡保种的方法
CN116210647A (zh) * 2023-02-03 2023-06-06 威奥福生物科技(宁波)有限公司 一种使用原始生殖细胞干细胞系扩繁白羽肉鸡的方法

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Also Published As

Publication number Publication date
EP2500417A1 (en) 2012-09-19
CN101506354B (zh) 2014-05-07
NZ574678A (en) 2012-03-30
US20150225695A1 (en) 2015-08-13
MX2009001477A (es) 2009-02-18
JP5405302B2 (ja) 2014-02-05
CN101506354A (zh) 2009-08-12
AU2007283564B2 (en) 2013-07-25
WO2008017704A1 (en) 2008-02-14
EP2054507A1 (en) 2009-05-06
US20100235937A1 (en) 2010-09-16
JP2010500015A (ja) 2010-01-07
AU2007283564A1 (en) 2008-02-14
BRPI0714257A2 (pt) 2013-12-03
RU2473688C2 (ru) 2013-01-27
US8962311B2 (en) 2015-02-24
RU2009107854A (ru) 2010-09-27
CA2660035A1 (en) 2008-02-14
KR101567095B1 (ko) 2015-11-06
KR20090038464A (ko) 2009-04-20

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