BRPI0410520B8 - produção de proteínas de fusão de glucocerebrosidase (gcd) glicosilada em cultura de plantas - Google Patents

produção de proteínas de fusão de glucocerebrosidase (gcd) glicosilada em cultura de plantas

Info

Publication number
BRPI0410520B8
BRPI0410520B8 BRPI0410520A BRPI0410520A BRPI0410520B8 BR PI0410520 B8 BRPI0410520 B8 BR PI0410520B8 BR PI0410520 A BRPI0410520 A BR PI0410520A BR PI0410520 A BRPI0410520 A BR PI0410520A BR PI0410520 B8 BRPI0410520 B8 BR PI0410520B8
Authority
BR
Brazil
Prior art keywords
production
specifically
gcd
glucocerebrosidase
proteins
Prior art date
Application number
BRPI0410520A
Other languages
English (en)
Inventor
Lewkowicz Ayala
Bartfeld Daniel
Baum Gideon
Hashmueli Sharon
Shaaltiel Yoseph
Original Assignee
Protalix Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Protalix Ltd filed Critical Protalix Ltd
Publication of BRPI0410520A publication Critical patent/BRPI0410520A/pt
Publication of BRPI0410520B1 publication Critical patent/BRPI0410520B1/pt
Publication of BRPI0410520B8 publication Critical patent/BRPI0410520B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/02—Drugs for disorders of the nervous system for peripheral neuropathies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
    • A61P31/22—Antivirals for DNA viruses for herpes viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01045—Glucosylceramidase (3.2.1.45), i.e. beta-glucocerebrosidase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Communicable Diseases (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Diabetes (AREA)
  • Oncology (AREA)
  • Obesity (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

produção de proteínas de alta manose em cultura de plantas, especificamente proteínas com alta glicosilação de manose, ao mesmo tempo em que objetiva tais proteínas com sinal de re e/ou passando pelo golgi, a invenção se refere ainda aos vetores e métodos de expressão e produção de enzimas lisossômicas de alta manose enzimaticamente ativas usando raiz de planta transgênica, especificamente células de cenouras; mais especificamente, a invenção se refere a células hospedeiras, especificamente células de cenouras suspensas transgênicas, vetores e métodos para expressão de alta produção e produção de glicocerebrosidase (gcd) de alta manose biologicamente ativa; a invenção fornece ainda composições e métodos para tratamento de doenças de armazenamento lisossômico.
BRPI0410520A 2003-04-27 2004-02-24 produção de proteínas de fusão de glucocerebrosidase (gcd) glicosilada em cultura de plantas BRPI0410520B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IL155.588 2003-04-27
IL15558803A IL155588A0 (en) 2003-04-27 2003-04-27 Methods for expression of enzymatically active recombinant lysosomal enzymes in transgenic plant root cells and vectors used thereby
PCT/IL2004/000181 WO2004096978A2 (en) 2003-04-27 2004-02-24 Production of high mannose proteins in plant culture

Publications (3)

Publication Number Publication Date
BRPI0410520A BRPI0410520A (pt) 2006-06-20
BRPI0410520B1 BRPI0410520B1 (pt) 2021-02-23
BRPI0410520B8 true BRPI0410520B8 (pt) 2021-05-25

Family

ID=32697072

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0410520A BRPI0410520B8 (pt) 2003-04-27 2004-02-24 produção de proteínas de fusão de glucocerebrosidase (gcd) glicosilada em cultura de plantas

Country Status (21)

Country Link
US (1) US20060204487A1 (pt)
EP (6) EP2322636B1 (pt)
JP (2) JP4993693B2 (pt)
KR (3) KR20110089193A (pt)
CN (2) CN1875111B (pt)
AU (1) AU2004234635B2 (pt)
BR (1) BRPI0410520B8 (pt)
CA (1) CA2523539C (pt)
CY (1) CY1114893T1 (pt)
DK (1) DK2333083T3 (pt)
ES (2) ES2446965T3 (pt)
HK (1) HK1088041A1 (pt)
IL (3) IL155588A0 (pt)
MX (1) MXPA05011507A (pt)
PT (1) PT2333083E (pt)
RU (2) RU2385928C3 (pt)
SG (2) SG2013021217A (pt)
SI (1) SI2333083T1 (pt)
UA (1) UA89944C2 (pt)
WO (1) WO2004096978A2 (pt)
ZA (1) ZA200508680B (pt)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050032211A1 (en) * 1996-09-26 2005-02-10 Metabogal Ltd. Cell/tissue culturing device, system and method
US20030100945A1 (en) 2001-11-23 2003-05-29 Mindguard Ltd. Implantable intraluminal device and method of using same in treating aneurysms
US7951557B2 (en) * 2003-04-27 2011-05-31 Protalix Ltd. Human lysosomal proteins from plant cell culture
WO2006094267A2 (en) * 2005-03-03 2006-09-08 Indiana University Research And Technology Corporation Permethylation of oligosaccharides
AU2006271181B2 (en) * 2005-07-18 2012-07-12 Protalix Ltd. Mucosal or enteral administration of biologically active macromolecules
EP2077855B1 (en) 2006-10-31 2012-02-01 Yildiz, Dr. med., Yildiz Therapeutic use of gba2
BRPI0810343B1 (pt) 2007-05-07 2021-03-30 Protalix Ltd Dispositivo descartável para o cultivo e colheita de tecidos vegetais e/ou células, método de cultivo e colheita de um tecido vegetal e/ou células vegetais em um volume maior do que 400 litros e sistema de cultivo da célula vegetal
ES2564685T3 (es) * 2007-07-03 2016-03-28 Idemitsu Kosan Co., Ltd. Vacuna contra la enfermedad de edema porcino
BRPI0815324A2 (pt) * 2007-08-20 2015-07-14 Protalix Ltd "conjugado protêico que contém sacarídeos, processo de separação do conjugado protêico que contém sacarídeos, composição farmacêutica do conjugado protêico que contém sacarídeos, uso do conjugado protêico que contém sacarídeos e composto do conjugado protêico que contém sacarídeos"
ITUD20080055A1 (it) * 2008-03-13 2009-09-14 Transactiva S R L Procedimento per la produzione di una proteina umana in pianta, in particolare un enzima lisosomiale umano ricombinante in endosperma di cereali
JP5305284B2 (ja) * 2008-03-26 2013-10-02 独立行政法人産業技術総合研究所 糖化合物の質量分析法
EP3009145A1 (en) 2009-03-30 2016-04-20 Mount Sinai School of Medicine of New York University Influenza virus vaccines and uses thereof
CN105126086B (zh) 2009-07-28 2019-05-14 夏尔人类遗传性治疗公司 用于治疗戈谢病的组合物和方法
EP2470199B1 (en) 2009-08-28 2019-05-22 Icahn School of Medicine at Mount Sinai Dose escalation enzyme replacement therapy for treating acid sphingomyelinase deficiency
US9194011B2 (en) 2009-11-17 2015-11-24 Protalix Ltd. Stabilized alpha-galactosidase and uses thereof
US20120230974A1 (en) 2009-11-17 2012-09-13 Protalix Ltd Alkaline alpha galactosidase for the treatment of fabry disease
WO2011107991A1 (en) 2010-03-02 2011-09-09 Protalix Ltd. Glucocerebrosidase multimers and uses thereof
EP3248615A1 (en) 2010-03-30 2017-11-29 Mount Sinai School of Medicine of New York University Influenza virus vaccines and uses thereof
WO2012001641A1 (en) * 2010-06-30 2012-01-05 Actelion Pharmaceuticals Ltd Stable pharmaceutical compositions
WO2012050175A1 (ja) 2010-10-15 2012-04-19 日本ケミカルリサーチ株式会社 糖鎖の非還元末端がマンノース残基である糖蛋白質の製造方法
LT3272861T (lt) * 2011-01-20 2020-03-25 Protalix Ltd. Alfa-galaktozidazės kompozicijos
KR101158673B1 (ko) * 2011-06-24 2012-07-03 주식회사 지씨바이오 재조합 인간 이듀로네이트-2-설파타아제를 포함하는 조성물, 제제 및 이의 제조방법
US20160145589A1 (en) 2011-06-24 2016-05-26 Green Cross Corporation Composition and formulation comprising recombinant human iduronate-2-sulfatase and preparation method thereof
IN2014CN02114A (pt) 2011-09-20 2015-05-29 Sinai School Medicine
EP2814838A1 (en) 2012-02-19 2014-12-24 Protalix Ltd. Oral unit dosage forms and uses of same for the treatment of gaucher disease
KR20140138850A (ko) 2012-03-02 2014-12-04 샤이어 휴먼 지네틱 테라피즈 인크. Ｉｉｉ형 고셔병을 치료하기 위한 조성물 및 방법
EP4154907A1 (en) 2012-12-18 2023-03-29 Icahn School of Medicine at Mount Sinai Influenza virus vaccines and uses thereof
JP6062074B2 (ja) * 2013-02-28 2017-01-18 ウンファ コーポレイション 組換え植物細胞、これの製造方法及びこれを利用した目的タンパク質の生産方法
WO2014136113A1 (en) 2013-03-06 2014-09-12 Protalix Ltd. Chimeric polypeptides, polynucleotides encoding same, cells expressing same and methods of producing same
US9908930B2 (en) 2013-03-14 2018-03-06 Icahn School Of Medicine At Mount Sinai Antibodies against influenza virus hemagglutinin and uses thereof
EP2789686A1 (en) * 2013-04-11 2014-10-15 Greenovation Biotech GmbH Expression of phosphorylated glycoproteins in plants
WO2015118547A1 (en) 2014-02-10 2015-08-13 Protalix Ltd. Method of maintaining disease stability in a subject having gaucher's disease
EP3912635A1 (en) * 2014-07-11 2021-11-24 Biostrategies LC Materials and methods for treating disorders associated with sulfatase enzymes
US10000551B2 (en) 2014-09-11 2018-06-19 Protalix Ltd. Chimeric polypeptides, polynucleotides encoding same, cells expressing same and methods of producing same
EA038931B9 (ru) * 2014-11-20 2022-02-18 Йиссум Рисерч Дивелопмент Компани Оф Зе Хебрю Юниверсити Оф Иерусалим Лтд. Композиции и способы получения полипептидов с модифицированным профилем гликозилирования в клетках растений
WO2016118937A1 (en) 2015-01-23 2016-07-28 Icahn School Of Medicine At Mount Sinai Influenza virus vaccination regimens
CN107429257B (zh) * 2015-03-17 2022-02-15 艾丽法有限责任公司 糖基化溶酶体蛋白、生产方法和用途
EP3454964B1 (en) * 2016-05-13 2021-06-23 Agilent Technologies, Inc. Automated alignment, calibration and standardization of electrophoresis data
CN109641041A (zh) 2016-06-15 2019-04-16 西奈山伊坎医学院 流感病毒血细胞凝集素蛋白及其用途
CA3058652A1 (en) 2017-04-07 2018-10-11 Icahn School Of Medicine At Mount Sinai Anti-influenza b virus neuraminidase antibodies and uses thereof
RU2668158C1 (ru) * 2017-06-28 2018-09-26 Общество с ограниченной ответственностью "Международный Биотехнологический Центр "Генериум" Способ очистки имиглюцеразы

Family Cites Families (76)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US467993A (en) * 1892-02-02 Alfred jorgensen
US2147271A (en) * 1937-07-16 1939-02-14 Schwarz Lab Inc Pure yeast culture apparatus
US2341259A (en) * 1941-01-22 1944-02-08 Lummus Co Inoculating method and apparatus
US2836434A (en) * 1952-08-21 1958-05-27 Separator Ab Arrangement for sterile interconnection and sealing of apparatuses in a plant for instance for cultivation of bacteria
US3201327A (en) * 1962-08-21 1965-08-17 Sun Oil Co Fermentation apparatus and process
SE347015B (pt) * 1966-10-06 1972-07-24 Sanraku Ocean Co
US3468520A (en) * 1967-05-12 1969-09-23 American Cyanamid Co Combination agitation and transfer unit for suspended cells
US3504185A (en) * 1968-05-09 1970-03-31 Univ Syracuse Res Corp Apparatus for measuring and controlling cell population density in a liquid medium
GB1284077A (en) * 1968-10-18 1972-08-02 British Petroleum Co Process and apparatus for the cultivation of micro-organisms
US3540700A (en) * 1969-01-24 1970-11-17 New Brunswick Scientific Co Rotary processing apparatus
DE2059931C3 (de) * 1970-12-05 1973-10-18 Knapsack Ag, 5033 Huerth-Knapsack Verfahren zum Verhindern von Schaumausbrüchen aus geschlossenen Reaktionsräumen
US3950227A (en) * 1970-12-21 1976-04-13 St. John's University Batch method of establishing and maintaining a controlled aerobic environment for a microbial culture
US3793154A (en) * 1970-12-21 1974-02-19 Univ St Johns Apparatus for gaseous environmental control of batch cultures of micro-organisms
US4179339A (en) * 1977-03-02 1979-12-18 Olympus Optical Company, Ltd. Liquid feeder for automatic culture apparatus
US4228243A (en) * 1978-07-13 1980-10-14 Toray Industries, Inc. Cell culture propagation apparatus
US4328317A (en) * 1980-03-31 1982-05-04 Solargizer International, Inc. Continuous chemical conversion or fermentation apparatus
DE3133314A1 (de) * 1981-08-22 1983-03-10 Hoechst Ag, 6000 Frankfurt "vorrichtung zum dispergieren einer zweiten phase in einer ersten phase"
US4519984A (en) * 1982-01-18 1985-05-28 Phillips Petroleum Company Apparatus for carrying out sparged reaction
CH663422A5 (de) * 1982-05-27 1987-12-15 Chemap Ag Verfahren und fermenter zur zuechtung von gewebezellen.
US4708938A (en) * 1984-04-30 1987-11-24 Hickinbotham Winemakers Pty. Ltd. Alcoholic fermentation
US4668632A (en) * 1985-02-06 1987-05-26 Vxr, Inc. Sparger and apparatus for and method of growing cells
US5372945A (en) * 1985-06-06 1994-12-13 Alchas; Paul G. Device and method for collecting and processing fat tissue and procuring microvessel endothelial cells to produce endothelial cell product
CN85100412B (zh) * 1985-06-25 1988-02-10 山东省食品发酵工业研究所 用嗜热放线菌生产固定化葡萄糖异构酶的方法及其用于高果糖糖浆的生产方法
US4713345A (en) * 1985-07-29 1987-12-15 Grain Processing Corporation Fermentation methods and apparatus
EP0224962A1 (en) * 1985-11-25 1987-06-10 De Staat Der Nederlanden Vertegenwoordigd Door De Minister Van Welzijn, Volksgezondheid En Cultuur Apparatus for the continuous cultivation of microorganisms in a culture liquid
US5166072A (en) * 1986-06-26 1992-11-24 Bayer Aktiengesellschaft Apparatus for the cultivation of immobilized micro-organisms
US4717668A (en) * 1986-12-12 1988-01-05 Baxter Travenol Laboratories, Inc. Plastic roller bottle for suspension cultures
US5081036A (en) * 1987-01-23 1992-01-14 Hoffmann-La Roche Inc. Method and apparatus for cell culture
US4908315A (en) * 1987-03-04 1990-03-13 Agristar, Inc. Integument and method for micropropagation and tissue culturing
KR970001000B1 (ko) * 1987-11-28 1997-01-25 닛본 신야쿠 가부시기가이샤 아미노디옥시만니톨의 제법
US6846968B1 (en) * 1988-02-26 2005-01-25 Large Scale Biology Corporation Production of lysosomal enzymes in plants by transient expression
US4931401A (en) * 1988-09-01 1990-06-05 La Societe De Recherche Snc Inc. Bioreactor
US5073491A (en) * 1988-12-23 1991-12-17 Hoffman-La Roche Inc. Immobilization of cells in alginate beads containing cavities for growth of cells in airlift bioreactors
US5549892A (en) * 1988-12-23 1996-08-27 Genzyme Corporation Enhanced in vivo uptake of glucocerebrosidase
US5100801A (en) * 1989-01-26 1992-03-31 Biocontrol Systems, Inc. Device for sequential microbial enrichment in a single apparatus
US5188946A (en) * 1989-01-26 1993-02-23 Biocontrol Systems Incorporated Process and device for sequential microbial enrichment in a single apparatus
JP2514102B2 (ja) * 1990-06-13 1996-07-10 ユリカ株式会社 ユリ科アロエ属に属する植物の真空凍結乾燥体を利用した錠剤製品及びその製造方法
ATE290087T1 (de) * 1990-06-15 2005-03-15 Syngenta Participations Ag Neue signalsequenzen
EP0471947A1 (en) * 1990-06-29 1992-02-26 Sekisui Chemical Co., Ltd. Culture bag
US5240598A (en) * 1990-09-18 1993-08-31 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Microbubble generator for the transfer of oxygen to microbial inocula and microbubble generator immobilized cell reactor
US5401650A (en) * 1990-10-24 1995-03-28 The Mount Sinai School Of Medicine Of The City University Of New York Cloning and expression of biologically active α-galactosidase A
US5367110A (en) 1990-11-13 1994-11-22 Yeda Research And Development Co. Ltd. Transgenic plants overproducing threonine and lysine
AT394576B (de) * 1991-01-16 1992-05-11 Vogelbusch Gmbh Reaktor zur durchfuehrung biologischer reaktionen mittels biokatalysatoren
US5612188A (en) * 1991-11-25 1997-03-18 Cornell Research Foundation, Inc. Automated, multicompartmental cell culture system
US5267791A (en) * 1991-12-13 1993-12-07 Corning Incorporated Suspended cell culture stirring vessel closure and apparatus
JPH05219834A (ja) * 1992-02-14 1993-08-31 Fumiko Kobayashi ディスポーサブル培養袋及び培養方法
IL102189A (en) * 1992-06-12 1995-07-31 Univ Ben Gurion Device for growing microorganisms
US5409833A (en) * 1993-07-01 1995-04-25 Baxter International Inc. Microvessel cell isolation apparatus
US5342781A (en) * 1993-07-15 1994-08-30 Su Wei Wen W External-loop perfusion air-lift bioreactor
NZ297142A (en) * 1994-10-24 2001-09-28 Texas A & M Univ Sys Oral immunisation and oral vaccines using edible transgenic plants comprising immunogenic agents such as E coli and cholera toxin antigens
DE69637875D1 (de) * 1995-09-14 2009-04-30 Virginia Tech Intell Prop Produktion von lysosomalen enzymen in pflanzlichen expressionssystemen
CA2246242A1 (en) * 1996-02-08 1997-08-14 Institut Fur Pflanzengenetik Und Kulturpflanzenforschung Cassettes for the expression of storable proteins in plants
US6458574B1 (en) * 1996-09-12 2002-10-01 Transkaryotic Therapies, Inc. Treatment of a α-galactosidase a deficiency
US6083725A (en) * 1996-09-13 2000-07-04 Transkaryotic Therapies, Inc. Tranfected human cells expressing human α-galactosidase A protein
IL119310A (en) 1996-09-26 1999-07-14 Metabogal Ltd Cell/tissue culturing device and method
US20050032211A1 (en) * 1996-09-26 2005-02-10 Metabogal Ltd. Cell/tissue culturing device, system and method
US6190913B1 (en) * 1997-08-12 2001-02-20 Vijay Singh Method for culturing cells using wave-induced agitation
US6096546A (en) * 1998-01-30 2000-08-01 Board Of Trustees, Rutgers, The State University Of New Jersey Methods for recovering polypeptides from plants and portions thereof
WO2000011953A1 (en) * 1998-09-01 2000-03-09 Penn State Research Foundation Method and apparatus for aseptic growth or processing of biomass
CA2344269A1 (en) * 1998-10-07 2000-04-13 Syngenta Participations Ag Therapeutically active proteins in plants
US6432698B1 (en) * 1999-01-06 2002-08-13 Rutgers, The State University Disposable bioreactor for culturing microorganisms and cells
JP2002000248A (ja) * 2000-06-23 2002-01-08 Daiichi Kagaku Kk 凍結法及び凍結乾燥法
CA2417415C (en) * 2000-07-31 2012-10-09 Biolex, Inc. Expression of biologically active polypeptides in duckweed
US7138262B1 (en) * 2000-08-18 2006-11-21 Shire Human Genetic Therapies, Inc. High mannose proteins and methods of making high mannose proteins
CA2429210A1 (en) * 2000-11-15 2002-05-23 Jason C. Goodrick Expression system for recombinant proteins
WO2002081695A2 (en) * 2001-04-06 2002-10-17 Syngenta Participations Ag Genes that are modulated by posttranscriptional gene silencing
JP2004529645A (ja) * 2001-04-18 2004-09-30 フラームス・インテルウニフェルシタイル・インステイチュート・フォール・ビオテヒノロヒー・ヴェーゼットウェー 生物学的細胞での２次代謝物の生産促進のための膜トランスポーターポンプをコードする遺伝子の使用
US7629309B2 (en) * 2002-05-29 2009-12-08 Zystor Therapeutics, Inc. Targeted therapeutic proteins
ES2224792B1 (es) * 2002-06-28 2007-02-16 Era Plantech, S.L. Produccion de peptidos y proteinas por acumulacion de cuerpos proteicos derivados de reticulos endoplasmico en plantas.
US7951557B2 (en) * 2003-04-27 2011-05-31 Protalix Ltd. Human lysosomal proteins from plant cell culture
EP1682576A1 (en) * 2003-11-06 2006-07-26 Compugen Ltd. Variants of human glycoprotein hormone alpha chain: compositions and uses thereof
DE602005007423D1 (de) * 2004-06-02 2008-07-24 Millipore Corp Einwegreaktor
KR101021203B1 (ko) * 2004-06-04 2011-03-11 크셀렉스, 인크. 1회용 바이오리액터 장치 및 방법
CA2583691A1 (en) * 2004-10-13 2006-04-20 Protalix Ltd. System and method for production of antibodies in plant cell culture
US20080132743A1 (en) * 2006-12-01 2008-06-05 Albemarle Corporation Production of high purity decabromodiphenylalkanes
BRPI0810343B1 (pt) * 2007-05-07 2021-03-30 Protalix Ltd Dispositivo descartável para o cultivo e colheita de tecidos vegetais e/ou células, método de cultivo e colheita de um tecido vegetal e/ou células vegetais em um volume maior do que 400 litros e sistema de cultivo da célula vegetal

Also Published As

Publication number Publication date
EP2322636B1 (en) 2015-04-08
EP2333083A1 (en) 2011-06-15
ZA200508680B (en) 2008-10-29
RU2385928C9 (ru) 2021-07-19
US20060204487A1 (en) 2006-09-14
RU2535342C2 (ru) 2014-12-10
WO2004096978A2 (en) 2004-11-11
PT2333083E (pt) 2014-02-26
RU2009148012A (ru) 2011-06-27
IL171561A (en) 2014-09-30
UA89944C2 (ru) 2010-03-25
AU2004234635B2 (en) 2010-05-13
CN1875111B (zh) 2011-06-29
EP2322636A1 (en) 2011-05-18
CA2523539C (en) 2017-06-06
EP1618177B1 (en) 2012-06-06
EP2330205B1 (en) 2015-01-21
SG2013021217A (en) 2015-08-28
IL155588A0 (en) 2003-11-23
JP2011184445A (ja) 2011-09-22
RU2385928C3 (ru) 2021-12-27
BRPI0410520B1 (pt) 2021-02-23
BRPI0410520A (pt) 2006-06-20
JP2006524506A (ja) 2006-11-02
ES2446965T3 (es) 2014-03-11
CY1114893T1 (el) 2016-12-14
RU2385928C2 (ru) 2010-04-10
EP2330205A1 (en) 2011-06-08
CA2523539A1 (en) 2004-11-11
JP4993693B2 (ja) 2012-08-08
EP2343378A1 (en) 2011-07-13
EP2330204A1 (en) 2011-06-08
KR20120093964A (ko) 2012-08-23
IL182888A0 (en) 2007-08-19
RU2005136874A (ru) 2006-06-10
SI2333083T1 (sl) 2014-03-31
KR20060018826A (ko) 2006-03-02
DK2333083T3 (da) 2014-01-27
EP1618177A2 (en) 2006-01-25
CN1875111A (zh) 2006-12-06
SG2007172737A (en) 2014-09-26
ES2391057T3 (es) 2012-11-21
KR101332372B1 (ko) 2013-11-27
EP2333083B1 (en) 2014-01-01
CN102240397A (zh) 2011-11-16
AU2004234635A1 (en) 2004-11-11
KR20110089193A (ko) 2011-08-04
MXPA05011507A (es) 2006-07-03
EP2343378B1 (en) 2014-09-10
IL182888A (en) 2014-08-31
WO2004096978A3 (en) 2006-03-30
EP1618177A4 (en) 2007-07-04
HK1088041A1 (en) 2006-10-27

Similar Documents

Publication Publication Date Title
BRPI0410520B8 (pt) produção de proteínas de fusão de glucocerebrosidase (gcd) glicosilada em cultura de plantas
MX2009011751A (es) Produccion de proteinas de alto contenido de manosa en cultivo de plantas.
Pauly et al. Biosynthesis of the plant cell wall matrix polysaccharide xyloglucan
Majda et al. The role of auxin in cell wall expansion
Atmodjo et al. Galacturonosyltransferase (GAUT) 1 and GAUT7 are the core of a plant cell wall pectin biosynthetic homogalacturonan: galacturonosyltransferase complex
Stern Devising a pathway for hyaluronan catabolism: are we there yet?
BR0111979A (pt) Composição para degradar um oligossacarìdeo, método para degradar um oligossacarìdeo, célula hospedeira, métodos para realçar a degradação de um oligossacarìdeo, para fabricar uma célula hospedeira recombinante, para expressar uma endoglicanase em uma célula hospedeira, e para produzir etanol a partir de uma fonte de oligossacarìdeo, vetor, método para degradar um oligossacarìdeo, e, extrato de enzima
Sista Kameshwar et al. Understanding the structural and functional properties of carbohydrate esterases with a special focus on hemicellulose deacetylating acetyl xylan esterases
BR9915026A (pt) Móleculas de ácido nucléico codificando uma enzima ramificadora de bactérias do gênero neisseria bem como métodos para a produção de alfa-1,4 glicanos ramificados para alfa-1,6
Watanabe et al. Sialylation of N-glycans on the recombinant proteins expressed by a baculovirus-insect cell system under β-N-acetylglucosaminidase inhibition
Urbanowicz et al. A tomato endo-β-1, 4-glucanase, SlCel9C1, represents a distinct subclass with a new family of carbohydrate binding modules (CBM49)
MXPA04006357A (es) Metodos para disenar estructuras de carbohidrato del tipo de mamifero.
BR0010989A (pt) Plantas e células de plantas geneticamente modificadas com uma atividade aumentada de uma proteìna de amilossacarase e uma enzima de ramificação
ES2246227T8 (es) Moléculas de ácido nucleico que codifican alternansacarasa
WO2006107990A3 (en) Improving protein n-glycosylation of eukaryotic cells using dolichol-linked oligosaccharide synthesis pathway, other n-gylosylation-increasing methods, and engineered hosts expressing products with increased n-glycosylation
AR050299A1 (es) Plantas con una enzima fosforilante del almidon con una actividad aumentada en plástidos.
BR0316380A (pt) Produção de proteìna e óleo aumentada em plantas pelo rompimento da trilha de fenilpropanóide
BR0011499A (pt) Moléculas de ácido nucléico oriundas de plantas e células de,planta transgênicas de trigo e o seu uso para a produção de amido modificado
ATE455860T1 (de) Expression von klasse ii mannosidase und klasse iii mannosidase in zellen niederer eukaryoten
WO2000005381A3 (en) Expression of enzymes involved in cellulose modification
BR0112643A (pt) Tioesterases de acil coenzima a
CN101085345A (zh) 高甘露糖蛋白质和产生高甘露糖蛋白质的方法
BR0009471A (pt) Composições e processos para modificação de transcrição de gene
BR0215851A (pt) molécula de dna codificando quitinase de algodão expressa preferencialmente em fibras durante deposição da parede celular secundária e o promotor correspondente
Kaku et al. Action of xyloglucan hydrolase within the native cell wall architecture and its effect on cell wall extensibility in azuki bean epicotyls

Legal Events

Date Code Title Description
B15K Others concerning applications: alteration of classification

Free format text: ALTERADA DA INT. CL. C12P 21/06, C12N 9/00, C12N 9/14, C12N 1/12, C12N 1/20, C12N 5/00, C12N 15/00, C07H 21/04, A01H 11/00.

Ipc: C12N 15/82 (2006.01), C12P 21/02 (2006.01), C12N 1

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B07E Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B07C Technical examination (opinion): republication [chapter 7.3 patent gazette]

Free format text: REPUBLIQUE-SE (7.3)

B09B Patent application refused [chapter 9.2 patent gazette]
B12B Appeal against refusal [chapter 12.2 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 23/02/2021, OBSERVADAS AS CONDICOES LEGAIS.

B16C Correction of notification of the grant [chapter 16.3 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 24/02/2004 OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF