JP5913372B2 - 植物および植物細胞におけるα−ガラクトシダーゼの発現のための核酸発現構築物 - Google Patents
植物および植物細胞におけるα−ガラクトシダーゼの発現のための核酸発現構築物 Download PDFInfo
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Description
ヒトα−ガラクトシダーゼタンパク質(EC3.2.1−22 GenBank:X05790)をコードするcDNAが、GENEART AG(Regensburg、ドイツ)により最適化され、合成された。コドンの使用を、リーダーペプチド(小胞体標的シグナルペプチド)を除いて、タバコ(Nicotiana tabacum)遺伝子のコドンの偏りに適応させた。最適化プロセスの間、以下のシス作用性の配列モチーフは回避した:内部TATA−ボックス、カイ部位(chi−site)およびリボゾーム進入部位、ATに富むまたはGCに富む配列ストレッチ、RNA不安定性エレメント(「キラーモチーフ」)、反復配列およびRNAの二次構造、スプライスドナー(陰性(cryptic))およびアクセプター部位、分枝点。さらに、GC含有量の非常に高い(>80%)または非常に低い(<30%)領域も回避されている。
ベンサミアナタバコ中の一過性の発現システム
成熟した植物全体における、タンパク質の、迅速な、高いレベルの一過性の発現を可能にする、植物ウイルスベクターの使用を、この場合には、トランスジェニック植物の代替物として選んだ。
ベンサミアナタバコ植物を、顆粒状の緩徐放出性肥料(Scott Marysville、OH)を補充した、市販のミックス土壌(Givaat Ada、IL)中、長日(16時間の明所/8時間の暗所)の光の形態下、24℃〜25℃で発芽および成長させた。
ベンサミアナタバコ葉の試料を、浸潤の5日後に収集し、SDS−PAGEのためのLaemmli緩衝液、または活性アッセイ用緩衝液(20mMクエン酸、30mMリン酸ナトリウム、0.1%BSAおよび0.67%エタノール、pH4.6)中に抽出し、植物が発現したタンパク質の触媒活性をアッセイした。
ヒトα−ガラクトシダーゼタンパク質を、植物抽出物から、二段階の硫酸アンモニウム示差沈殿(「塩析」:第1段階:0.57M;第2段階:2.27M)、続いて、疎水性相互作用クロマトグラフィー(Phenyl650M樹脂)およびカチオン交換クロマトグラフィーにより精製した。
外来遺伝子を植物細胞ゲノム内に導入するために、アグロバクテリウム媒介型形質転換が広く使用されている。この技法は、プラスミドDNAセグメント、すなわち、転移DNA(T−DNA)を宿主細胞ゲノム内に移入することによって、植物細胞を形質転換する、アグロバクテリウムの天然の能力に基づく。このアプローチを使用すると、外来遺伝子およびその調節エレメントからなるT−DNA分子が、植物ゲノム内に無作為に導入される。組込みの部位および遺伝子挿入体のコピー数は制御されず、したがって、形質転換プロセスの結果、導入遺伝子の発現の種々のレベルを有する細胞から構成されるトランスジェニック細胞の「プール」が生じる。続いて、トランスジェニックな「プール」を使用して、クローンの単離を行う。クローンの単離の結果、多くの単一細胞株が確立され、次いで、それらから、外来遺伝子の最も高い発現レベルを有するクローンを選択する。
ヒトα−ガラクトシダーゼタンパク質を発現する形質転換BY2細胞100mgを、20mM EDTA、2mM DTTおよび2mM PMSFを含有する20mMリン酸緩衝液0.5M、pH=7.2、200ul中で5分間ホモジナイズした。ホモジネートを遠心分離し、組換えヒトα−ガラクトシダーゼタンパク質を含有する上清を収集した[未精製の抽出物]。未精製状態におけるα−ガラクトシダーゼ触媒活性を、PNP−Gアッセイにより決定した。
α−ガラクトシダーゼのガラクトシドヒドロラーゼ活性を、p−ニトロフェニルアルファ−D−ガラクトピラノシド(pNP−アルファ−D−Gal、GBB1290、IRIS Biotech、ドイツ)を加水分解基質として使用して決定した。アッセイ用緩衝液は、20mMクエン酸、30mMリン酸ナトリウム、0.1%BSAおよび0.67%エタノールをpH4.6で含有した。試料(50μl)を、150μlのアッセイ用緩衝液と共にインキュベートした。基質(pNP−アルファ−D−Gal)を添加して(30μL)、8mMの最終濃度のp−NP−アルファ−D Galを得た。反応混合物を、37℃で90分間インキュベートした。90分後に、反応を止め、p−ニトロフェノラート発色団の発生を可能にするために、各ウエルに、100μlの炭酸ナトリウム(2M)を添加することによって、反応をクエンチした。405nmにおいて測定した吸光度から、結果を計算した。
異なるα−ガラクトシダーゼ構築物間での比較を行ったところ、リンゴペクチナーゼ小胞体標的化シグナルペプチド(配列番号10によりコードされるリンゴペクチナーゼER標的化シグナルペプチド)を用いて発現させたα−ガラクトシダーゼ配列(配列番号8もしくは配列番号11によりコードされるα−ガラクトシダーゼ−リンゴペクチナーゼERシグナルペプチド−タンパク質)、またはエンドキチナーゼB小胞体標的化シグナルペプチド(配列番号15によりコードされるエンドキチナーゼシグナルタンパク質、配列番号14)を用いて発現させたα−ガラクトシダーゼタンパク質と比較した場合、ABPI小胞体標的化シグナルペプチド(配列番号4)の、ヒト組換えα−ガラクトシダーゼポリペプチドへの付加により、一過性発現型のベンサミアナタバコ植物における組換えタンパク質の発現が改善される(図2、レーン2)ことが解明された。触媒活性のアッセイ(上記を参照されたい)により、リンゴペクチナーゼ小胞体標的化シグナルペプチドを用いて発現させたα−ガラクトシダーゼ配列、またはエンドキチナーゼB小胞体標的化シグナルペプチド(配列番号15によりコードされるエンドキチナーゼシグナルタンパク質、配列番号14)を用いて発現させたα−ガラクトシダーゼタンパク質と比較した場合の、図2に認められるタンパク質の増加は、葉1kg当たりの酵素活性が同時に増加すること(図3、カラムB)に反映されることも確認された。
植物が発現したヒト組換えα−ガラクトシダーゼの薬物動態に近づくために、組換え酵素の調製物を、シミュレートしたin−vivoにおける条件に曝し、触媒活性をモニターした。
組換え植物抽出物または植物抽出物から精製したα−ガラクトシダーゼを、ヒト血漿に(例えば、10μlの抽出物または精製したα−ガラクトシダーゼをく190μlのヒト血漿に)添加して、1μg/mlの最終濃度を得、37℃でインキュベートし、続いて、試料中のα−ガラクトシダーゼ触媒活性を、設定した間隔で1時間にわたり測定することによって、血漿中の酵素活性の安定性を評価した。
Claims (22)
- ヒトα−ガラクトシダーゼタンパク質をコードする核酸配列を含む核酸発現構築物であって、
前記ヒトα−ガラクトシダーゼタンパク質は、N末端においてシロイヌナズナABPI小胞体標的化シグナルペプチドに翻訳時に融合され、
前記ヒトα−ガラクトシダーゼタンパク質は、C末端において小胞体保持シグナルペプチドに翻訳時に融合される、核酸発現構築物。 - 前記核酸配列が、配列番号22を含む、請求項1に記載の核酸発現構築物。
- 前記シロイヌナズナABPI小胞体標的化シグナルペプチドが、配列番号4に記載されたものである、請求項1または2に記載の核酸発現構築物。
- 前記シロイヌナズナABPI小胞体標的化シグナルペプチドをコードする核酸配列が配列番号5に記載されたものである、請求項1〜3のいずれか一項に記載の核酸発現構築物。
- 前記小胞体保持シグナルペプチドが、配列番号6に記載されたものである、請求項1〜4のいずれか一項に記載の核酸発現構築物。
- 前記小胞体保持ペプチドをコードする核酸配列が配列番号19に記載されたものである、請求項1〜5のいずれか一項に記載の核酸発現構築物。
- 前記核酸配列が、配列番号2に記載される配列を有する、請求項1〜6のいずれか一項に記載の核酸発現構築物。
- 前記ヒトα−ガラクトシダーゼタンパク質が、配列番号1に記載されるアミノ酸配列を有する、請求項1〜7のいずれか一項に記載の核酸発現構築物。
- 請求項1〜8のいずれか一項に記載の核酸発現構築物を含む、単離細胞。
- 前記細胞が、植物細胞である、請求項9に記載の細胞。
- 前記植物細胞が、タバコ細胞である、請求項10に記載の細胞。
- 前記タバコ細胞が、BY−2細胞である、請求項11に記載の細胞。
- 組換えヒトα−ガラクトシダーゼタンパク質を製造する方法であって、
請求項9〜12のいずれか一項に記載の細胞を提供するステップと、
前記細胞を成長させて、前記組換えヒトα−ガラクトシダーゼタンパク質を産生するステップと、
前記細胞から、前記組換えヒトα−ガラクトシダーゼタンパク質を単離するステップと
を含む方法。 - N末端グリシン残基を有するヒトα−ガラクトシダーゼタンパク質であって、C末端において小胞体保持シグナルペプチドに翻訳時に融合されたヒトα−ガラクトシダーゼタンパク質。
- 配列番号16に記載されるアミノ酸配列を有する、請求項14に記載のヒトα−ガラクトシダーゼタンパク質。
- 活性成分としての請求項14または15に記載のヒトα−ガラクトシダーゼタンパク質、および薬学的に許容できる担体を含む医薬組成物。
- 前記ヒトα−ガラクトシダーゼタンパク質が、3つが露出したマンノース残基である9つのマンノース残基を含むグリカン構造を有する、請求項16に記載の医薬組成物。
- 活性成分として請求項14もしくは15または両項に記載のヒトα−ガラクトシダーゼタンパク質の集団を含む医薬組成物であって、前記集団の少なくとも0.5%が、3つが露出したマンノース残基である9つのマンノース残基を含むグリカン構造を有する、医薬組成物。
- 活性成分としての請求項14もしくは15または両項に記載のヒトα−ガラクトシダーゼタンパク質の集団、および薬学的に許容できる担体を含む医薬組成物であって、ヒトα−ガラクトシダーゼタンパク質の前記集団の優勢なグリカン構造が、マンノース4−β−(1,2)キシロース(M4X)、マンノース3−β−(1,2)キシロース−α−(1,3)フコース[Fc(3)M3X]、およびマンノース8(M8)である、医薬組成物。
- 活性成分としての請求項9〜12のいずれか一項に記載の細胞、および薬学的に許容できる担体を含む、医薬組成物。
- その治療を必要とする対象においてファブリー病を治療するための薬剤を製造するための、請求項14または15に記載のヒトα−ガラクトシダーゼタンパク質の使用。
- その治療を必要とする対象においてファブリー病を治療するための薬剤を製造するための、請求項9〜12のいずれか一項に記載の細胞の使用。
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EP3272861B1 (en) | 2019-12-18 |
EP3272861A1 (en) | 2018-01-24 |
KR20140015330A (ko) | 2014-02-06 |
CN103443270B (zh) | 2017-06-06 |
SI3272861T1 (sl) | 2020-06-30 |
CN103443270A (zh) | 2013-12-11 |
BR112013018516A2 (pt) | 2019-05-21 |
EP2665814A1 (en) | 2013-11-27 |
PT3272861T (pt) | 2020-03-26 |
EP2665814B1 (en) | 2017-05-17 |
US20130295065A1 (en) | 2013-11-07 |
JP2014506450A (ja) | 2014-03-17 |
BR112013018516B1 (pt) | 2023-11-07 |
ES2774190T3 (es) | 2020-07-17 |
WO2012098537A8 (en) | 2013-08-08 |
PL3272861T3 (pl) | 2020-07-13 |
CA2824791A1 (en) | 2012-07-26 |
WO2012098537A1 (en) | 2012-07-26 |
AU2011356137A1 (en) | 2013-08-15 |
CY1122794T1 (el) | 2021-05-05 |
IL227552B (en) | 2018-05-31 |
DK3272861T3 (da) | 2020-03-23 |
KR101883803B1 (ko) | 2018-07-31 |
LT3272861T (lt) | 2020-03-25 |
RS60129B1 (sr) | 2020-05-29 |
DK2665814T3 (en) | 2017-09-11 |
IL227552A0 (en) | 2013-09-30 |
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