BRPI0406622A - Processo de coleta de poliidroxialcanoato altamente puro de células microbianas - Google Patents

Processo de coleta de poliidroxialcanoato altamente puro de células microbianas

Info

Publication number
BRPI0406622A
BRPI0406622A BR0406622-7A BRPI0406622A BRPI0406622A BR PI0406622 A BRPI0406622 A BR PI0406622A BR PI0406622 A BRPI0406622 A BR PI0406622A BR PI0406622 A BRPI0406622 A BR PI0406622A
Authority
BR
Brazil
Prior art keywords
pha
highly pure
collection process
microbial cells
collection
Prior art date
Application number
BR0406622-7A
Other languages
English (en)
Inventor
Yoshifumi Yanagita
Noriko Sanseiso Ogawa
Yasuyoshi Ueda
Fumio Osakada
Keiji Matsumoto
Original Assignee
Kaneka Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kaneka Corp filed Critical Kaneka Corp
Publication of BRPI0406622A publication Critical patent/BRPI0406622A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids

Landscapes

  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Polyesters Or Polycarbonates (AREA)

Abstract

"PROCESSO DE COLETA DE POLIIDROXIALCANOATO ALTAMENTE PURO DE CéLULAS MICROBIANAS". Pretende-se apresentar um processo de separação e purificação de PHA, pelo qual constituintes celulares diferentes de partículas de PHA possam ser eficientemente removidos de células microbianas contendo PHA e, dessa forma, PHA altamente puro possa ser obtido em alto rendimento, sem causar qualquer diminuição séria do peso molecular, e um processo de obtenção de agregados de grãos de PHA. A saber, um processo de coleta de PHA que compreende a submissão de uma suspensão aquosa de células microbianas contendo PHA a um tratamento de ruptura mecânica a baixa temperatura e adição de um álcali para, dessa forma, romper eficientemente as células microbianas e, após a coleta do PHA, tratamento com uma enzima e/ou um surfatante. Além disso, a tamanho de partícula do PHA pode ser aumentado por suspensão do PHA em um solvente hidrofílico e/ou água e agitação da suspensão a uma temperatura abaixo de seu ponto de ebulição para, dessa forma, induzir a agregação.
BR0406622-7A 2003-01-20 2004-01-20 Processo de coleta de poliidroxialcanoato altamente puro de células microbianas BRPI0406622A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2003011099 2003-01-20
PCT/JP2004/000416 WO2004065608A1 (ja) 2003-01-20 2004-01-20 微生物菌体からの高純度ポリヒドロキシアルカノエートの回収方法

Publications (1)

Publication Number Publication Date
BRPI0406622A true BRPI0406622A (pt) 2005-12-06

Family

ID=32767270

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0406622-7A BRPI0406622A (pt) 2003-01-20 2004-01-20 Processo de coleta de poliidroxialcanoato altamente puro de células microbianas

Country Status (10)

Country Link
US (1) US7393668B2 (pt)
EP (1) EP1609868B1 (pt)
JP (1) JPWO2004065608A1 (pt)
CN (1) CN100572544C (pt)
BR (1) BRPI0406622A (pt)
CA (1) CA2512031A1 (pt)
PL (1) PL376468A1 (pt)
RU (1) RU2005121140A (pt)
TW (1) TW200508393A (pt)
WO (1) WO2004065608A1 (pt)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008193940A (ja) * 2007-02-13 2008-08-28 Honda Motor Co Ltd ポリヒドロキシ酪酸精製方法
PT103714B (pt) * 2007-04-11 2020-07-28 73100 - Setenta E Três Mil E Cem, Lda. Processo para a obtenção de um polímero à base de galactose
BRPI0913675A2 (pt) * 2008-07-02 2015-11-24 Basf Se método para isolar poli-hidroxialcanoatos das células de produção
LT2304040T (lt) * 2008-07-04 2017-11-27 Scientist Of Fortune S.A. Alkenų gamyba, fermentiniu būdu dekarboksilinant 3-hidroksialkano rūgštis
JP5651017B2 (ja) * 2008-12-09 2015-01-07 株式会社カネカ ポリ−３−ヒドロキシアルカン酸の生産方法
CN102224251B (zh) * 2008-12-09 2014-06-11 株式会社钟化 聚-3-羟基烷酸的凝集体的制备方法
JP5608096B2 (ja) * 2008-12-09 2014-10-15 株式会社カネカ ポリ−３−ヒドロキシアルカン酸及びその製造方法
MY157646A (en) * 2008-12-18 2016-07-15 Univ Putra Malaysia A method of extracting and purifying polyhydroxyalkanoate bioplastic
FR2951460B1 (fr) * 2009-10-16 2011-12-30 Valagro Carbone Renouvelable Poitou Charentes Procede de fabrication de polyhydroxyalcanoate
MY153891A (en) * 2010-03-01 2015-04-15 Univ Putra Malaysia A method for recovering an intracellular polyhydroxyalkanoate (pha)
WO2013016566A1 (en) * 2011-07-26 2013-01-31 Micromidas Inc. Methods of extracting polyhydroxyalkanoates from pha-containing bacterial cells
US9359283B2 (en) 2012-05-31 2016-06-07 Micromidas, Inc. Polyhydroxyalkanoate derivatives, preparation and uses thereof
US9290612B2 (en) 2013-03-13 2016-03-22 Tepha, Inc. Compositions and devices of poly-4-hydroxybutyrate
ITMI20131276A1 (it) 2013-07-30 2015-01-31 Bio On S R L Processo per recuperare e purificare poliidrossialcanoati da una coltura cellulare
EP3065789B1 (en) 2013-11-05 2018-10-24 Tepha, Inc. Compositions and devices of poly-4-hydroxybutyrate
JP6952031B2 (ja) * 2016-06-23 2021-10-20 株式会社カネカ ポリヒドロキシアルカン酸の製造方法
WO2018030499A1 (ja) 2016-08-10 2018-02-15 Ｓｐｉｂｅｒ株式会社 不溶性組換えタンパク質凝集体の製造方法
JP6993980B2 (ja) 2016-10-13 2022-02-04 株式会社カネカ ポリヒドロキシアルカン酸の製造方法
WO2018186278A1 (ja) * 2017-04-05 2018-10-11 株式会社カネカ ポリヒドロキシアルカノエート粒子及びその水分散液
WO2021059762A1 (ja) * 2019-09-25 2021-04-01 株式会社カネカ ポリヒドロキシアルカン酸の製造方法
CN111333822B (zh) * 2020-04-29 2022-04-08 中粮营养健康研究院有限公司 氨水结合超声提取聚羟基脂肪酸酯的方法和系统
CN111393625B (zh) * 2020-06-03 2020-09-01 中粮营养健康研究院有限公司 溶菌酶结合sds下利用超声提取聚羟基脂肪酸酯的方法和系统
TWI736413B (zh) * 2020-09-08 2021-08-11 行政院原子能委員會核能研究所 微生物萃取物之製備方法
US20240010789A1 (en) * 2020-11-24 2024-01-11 Kaneka Corporation Poly(3-hydroxyalkanoate) production method
CN112813112B (zh) * 2021-01-07 2022-11-15 上海碧州环保能源科技有限公司 一种以产pha为导向的非甲烷化工艺
WO2023278240A1 (en) * 2021-06-28 2023-01-05 Phb Industrial S.A. Methods to produce therapeutic formulations comprising hydroxybutirate and hydroxyvalerate, therapeutic formulations and uses thereof
JP2024531978A (ja) * 2021-08-23 2024-09-03 ダニマー・アイピーシーオー・エルエルシー バイオマスからｐｈａを回収するための方法
IT202200013483A1 (it) 2022-06-27 2023-12-27 Versalis Spa Procedimento per il recupero e la purificazione di poliidrossialcanoati da un brodo di fermentazione.
CN115322342B (zh) * 2022-08-12 2023-05-26 哈尔滨工业大学 序贯式同步回收活性污泥中聚羟基脂肪酸酯和海藻酸盐的方法
CN115807044B (zh) * 2022-11-09 2023-10-13 华南理工大学 一种高效提取并纯化高纯度聚羟基脂肪酸酯的方法
CN115786411B (zh) * 2023-01-09 2023-06-23 北京微构工场生物技术有限公司 聚羟基脂肪酸酯的提取方法
CN118307814A (zh) * 2024-05-15 2024-07-09 淮北矿业绿色化工新材料研究院有限公司 一种聚羟基脂肪酸颗粒的制备方法

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA80803B (en) 1979-02-21 1981-06-24 Ici Ltd Extraction process
EP0046017B1 (en) * 1980-08-13 1983-11-09 Imperial Chemical Industries Plc Extraction of poly(beta-hydroxy butyric acid)
US4358583A (en) * 1980-08-13 1982-11-09 Imperial Chemical Industries Plc Extraction of poly(β-hydroxy butyric acid)
US4477654A (en) * 1981-07-07 1984-10-16 Imperial Chemical Industries Plc 3-Hydroxybutyrate polymers
JPS63198991A (ja) 1987-02-13 1988-08-17 Mitsubishi Rayon Co Ltd ポリ−β−ヒドロキシ酪酸の分離精製法
AT390068B (de) 1988-07-07 1990-03-12 Danubia Petrochemie Extraktionsmittel fuer poly-d(-)-3-hydroxybuttersaeure
US5334520A (en) 1990-05-25 1994-08-02 Center For Innovative Technology Production of poly-beta-hydroxybutyrate in transformed escherichia coli
JPH0461638A (ja) 1990-06-27 1992-02-27 Ube Ind Ltd 光記録媒体
AT395319B (de) * 1990-10-05 1992-11-25 Danubia Petrochem Polymere Verfahren zur gewinnung eines polyhydroxyalkanoates aus dem zellmaterial eines mikroorganismus und polyhydroxyalkanoatflocken
WO1992022659A1 (en) 1991-06-11 1992-12-23 Kanebo, Ltd. Process for producing polyhydroxy organic acid ester
JPH05336982A (ja) 1992-06-11 1993-12-21 Kanebo Ltd ポリヒドロキシ有機酸エステルの分離・精製法
GB9215791D0 (en) * 1992-07-24 1992-09-09 Ici Plc Increasing the particle size of polymers
GB9222561D0 (en) 1992-10-27 1992-12-09 Ici Plc Macromolecular degradation
JPH0731487A (ja) 1993-07-14 1995-02-03 Asahi Chem Ind Co Ltd バイオポリエステル含有菌体からのバイオポリエステルの分離方法
JPH0731489A (ja) * 1993-07-15 1995-02-03 Asahi Chem Ind Co Ltd バイオポリエステル含有微生物からのバイオポリエステルの分離方法
JPH0731488A (ja) 1993-07-15 1995-02-03 Asahi Chem Ind Co Ltd バイオポリエステル含有微生物からのバイオポリエステル分離法
JP4126511B2 (ja) 1998-03-20 2008-07-30 三菱瓦斯化学株式会社 微生物菌体からのポリ−３−ヒドロキシ酪酸の回収方法
JP2000046094A (ja) 1998-07-30 2000-02-15 Kayaba Ind Co Ltd 油圧緩衝器
JP3930667B2 (ja) * 1999-08-10 2007-06-13 株式会社カネカ ポリ−３−ヒドロキシアルカン酸の分離精製方法
JP3930668B2 (ja) 1999-08-20 2007-06-13 株式会社カネカ ポリ−３−ヒドロキシアルカン酸の抽出方法
DE10013514C2 (de) * 2000-03-14 2002-06-27 Ufz Leipzighalle Gmbh Verfahren zur Gewinnung von Polyhydroxyalkanoaten (PHA) oder deren Copolymeren
JP4643799B2 (ja) * 2000-06-01 2011-03-02 株式会社カネカ ポリエステルの製造方法
US6808907B2 (en) * 2001-03-27 2004-10-26 Canon Kabushiki Kaisha Method and apparatus for producing polyhydroxyalkanoate
WO2003091444A1 (fr) * 2002-04-26 2003-11-06 Kaneka Corporation Procede de separation d'acide poly-3-hydroxyalcanoique

Also Published As

Publication number Publication date
CN1738904A (zh) 2006-02-22
CN100572544C (zh) 2009-12-23
RU2005121140A (ru) 2006-01-20
EP1609868B1 (en) 2015-11-11
WO2004065608A1 (ja) 2004-08-05
TW200508393A (en) 2005-03-01
US7393668B2 (en) 2008-07-01
EP1609868A4 (en) 2011-05-25
CA2512031A1 (en) 2004-08-05
PL376468A1 (en) 2005-12-27
EP1609868A1 (en) 2005-12-28
US20060084161A1 (en) 2006-04-20
JPWO2004065608A1 (ja) 2006-05-18

Similar Documents

Publication Publication Date Title
BRPI0406622A (pt) Processo de coleta de poliidroxialcanoato altamente puro de células microbianas
BR112012008850B1 (pt) processo para produção de materiais poliméricos biodegradáveis
Kosugi et al. Natural rubber with nanomatrix of non-rubber components observed by focused ion beam-scanning electron microscopy
CN106483228B (zh) 定量检测污泥中含硫氨基酸的方法
WO2016113933A1 (ja) セルロースナノ構造体及びその製造方法
CN105273831B (zh) 一种含油微藻的破壁及表面活性剂辅助提取油脂的方法
Rinanti et al. Harvesting of freshwater microalgae biomass by Scenedesmus sp. as bioflocculant
WO2018033814A1 (en) A process for the extraction of phycocyanin
Wang et al. Characteristics and distribution research on extracellular polymer substance extracted from sewage sludge
WO2018209416A1 (pt) Método de flotação de minerais utilizando biorreagente extraído de bactérias gram positivas
CN103773817B (zh) 一种利用茶皂素促进剩余污泥厌氧发酵产酸的方法
CN103675164B (zh) 分离检测环境中雌激素类内分泌干扰物的方法
CN100345861C (zh) 堆肥微生物总dna的提取与纯化方法
CN106414716B (zh) 伽马辐照稳定的葡聚糖溶液及使用方法
BR0314749A (pt) Método de coagulação do ácido poli-3-hidroxialcanóico
CN104211149A (zh) 一种用于纺织污水处理过程中的复合絮凝剂
JP2018145216A (ja) セルロースオリゴマーから成る三次元構造体の酵素合成
CN113941305B (zh) 一种适用于厌氧细菌的胞外聚合物提取方法
CN1053195C (zh) 从鸡血中提取鸡核酸及核蛋白的方法
CN102250887A (zh) 从关节软骨中提取rna的方法
JP2009195179A (ja) ポリヒドロキシアルカン酸抽出方法
US20090004727A1 (en) Method for the separation of microorganisms adhered to a solid sample by means of a phosphate-based damper and sonication of the sample
CN1544627A (zh) 生物大分子组分相分离法
Ribeiro et al. Sorting of spermatozoa with flow cytometry
Gruber et al. Improved yield of plasma membrane from mammalian cells through modifications of the two-phase polymer isolation procedure

Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 4A,5A,6A E 7A ANUIDADE(S).

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2112 DE 28/06/2011.