MY153891A - A method for recovering an intracellular polyhydroxyalkanoate (pha) - Google Patents

A method for recovering an intracellular polyhydroxyalkanoate (pha)

Info

Publication number
MY153891A
MY153891A MYPI2010000891A MYPI2010000891A MY153891A MY 153891 A MY153891 A MY 153891A MY PI2010000891 A MYPI2010000891 A MY PI2010000891A MY PI2010000891 A MYPI2010000891 A MY PI2010000891A MY 153891 A MY153891 A MY 153891A
Authority
MY
Malaysia
Prior art keywords
pha
pellet
freeze
distilled water
drying
Prior art date
Application number
MYPI2010000891A
Inventor
Mohd Ali Prof Dr Hassan
Siti Nor Syairah Binti Anis
Phang Lai Yee
Mitra Mohammadi
Nor Asma Abd Razak
Chong Mei Ling
Yoshihito Prof Shirai
Wan Md Zin Wan Prof Yunus
Assoc Prof Dr Suraini Abd Aziz
Amirul Al-Ashraf Dr Abdullah
Hasfalina Bt Che Dr Man
Original Assignee
Univ Putra Malaysia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Putra Malaysia filed Critical Univ Putra Malaysia
Priority to MYPI2010000891A priority Critical patent/MY153891A/en
Priority to PCT/MY2010/000333 priority patent/WO2011108916A2/en
Publication of MY153891A publication Critical patent/MY153891A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G63/00—Macromolecular compounds obtained by reactions forming a carboxylic ester link in the main chain of the macromolecule
    • C08G63/88—Post-polymerisation treatment
    • C08G63/89—Recovery of the polymer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

THE PRESENT INVENTION RELATES TO A METHOD TO RECOVER AN INTRACELLULAR POLYHYDROXYALKANOATES (PHA). THE METHOD NCIUDES THE STEPS OF (A) SEPARATING CULTURE MEDIUM CONTAINING PHA-RICH CELLS USING CENTRIFUGATION, (B) RINSING PELLEL OBTAINED FROM STEP (A) WITH DISTILLED WATER. (C) FREEZE- DRYING THE PELLET OBTAINED IN STEP (B), (D) ADDING DISTILLED WATER TO THE FREEZE-DRIED SAMPLE, (E) SEPARATING NON-PHA CELLULAR MATERIAL (NPCM) FROM PHA GRANULES, (F) WASHING THE PELLET CONTAINING PHA FROM STEP (E) BY DISTILLED WATER AND CENTRIFUGING FOR PRODUCING FINAL PELLET, AND (G) FREEZE-DRYING THE PELLET PRODUCED FROM STEP (F).
MYPI2010000891A 2010-03-01 2010-03-01 A method for recovering an intracellular polyhydroxyalkanoate (pha) MY153891A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
MYPI2010000891A MY153891A (en) 2010-03-01 2010-03-01 A method for recovering an intracellular polyhydroxyalkanoate (pha)
PCT/MY2010/000333 WO2011108916A2 (en) 2010-03-01 2010-12-30 A method for recovering an intracellular polyhydroxyalkanoate (pha)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MYPI2010000891A MY153891A (en) 2010-03-01 2010-03-01 A method for recovering an intracellular polyhydroxyalkanoate (pha)

Publications (1)

Publication Number Publication Date
MY153891A true MY153891A (en) 2015-04-15

Family

ID=44542763

Family Applications (1)

Application Number Title Priority Date Filing Date
MYPI2010000891A MY153891A (en) 2010-03-01 2010-03-01 A method for recovering an intracellular polyhydroxyalkanoate (pha)

Country Status (2)

Country Link
MY (1) MY153891A (en)
WO (1) WO2011108916A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107429274A (en) 2014-11-20 2017-12-01 全循环生物塑料有限公司 Polyhydroxy alkanoate copolymer is produced from debirs

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10013514C2 (en) * 2000-03-14 2002-06-27 Ufz Leipzighalle Gmbh Process for the production of polyhydroxyalkanoates (PHA) or their copolymers
EP1409706A2 (en) * 2000-09-13 2004-04-21 Biomatera Inc. Process for production of polyhydroxyalkanoate
WO2004033700A1 (en) * 2002-09-30 2004-04-22 Kaneka Corporation Method of coagulating poly-3-hydroxyalkanoic acid
TW200508393A (en) * 2003-01-20 2005-03-01 Kaneka Corp Method of collecting highly pure polyhydroxyalkanoate from microbial cells
JP4777778B2 (en) * 2003-12-19 2011-09-21 寧波天安生物材料有限公司 Method for directly separating, extracting and purifying poly-β-hydroxyalkanoates (PHAs) from bacterial fermentation broth
US7435567B2 (en) * 2004-03-04 2008-10-14 Kaneka Corporation Method for degradation of nucleic acids and use thereof
MY157646A (en) * 2008-12-18 2016-07-15 Univ Putra Malaysia A method of extracting and purifying polyhydroxyalkanoate bioplastic

Also Published As

Publication number Publication date
WO2011108916A2 (en) 2011-09-09
WO2011108916A3 (en) 2011-11-24

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